Miranda D. Grounds, Jessica R. Terrill, Hannah G. Radley‐Crabb, Terry Robertson, J. M. Papadimitriou, Simone Spuler, Tea Shavlakadze,
... in humans and myopathy in A/Jdys−/− and BLAJ mice, but the pathomechanism of the myopathy is ... muscles. Similar results were seen for dysferlin-deficient BLAJ mice. These novel observations of conspicuous intermyofibrillar lipid ... in humans and myopathy in A/Jdys−/− and BLAJ mice, but the pathomechanism of the myopathy is ... muscles. Similar results were seen for dysferlin-deficient BLAJ mice. These novel observations of conspicuous intermyofibrillar lipid ... A/J (A/Jdys−/−) mice and more recently BLAJ mice (B6.A-Dysf[prmd]/GeneJ, whereby the ...
Tópico(s): Nuclear Structure and Function
2014 - Elsevier BV | American Journal Of Pathology
Erin M. Lloyd, Hongyang Xu, Robyn M. Murphy, Miranda D. Grounds, Gavin J. Pinniger,
... longus; EDL) muscles using male dysferlin-deficient (dysf-/-) BLAJ mice aged 10 months, compared with wild type ( ... was a striking increase in muscle mass of BLAJ soleus (+25%) (p<0.001), with no strain ... no strain differences. Ex vivo measures showed the BLAJ soleus had faster twitch contraction (-21%) and relaxation (- ... post fatigue recovery (ps<0.05); whereas the BLAJ EDL had a slower relaxation time (+11%) and ... production (+25%) (ps 0.05); however, for the BLAJ EDL, there was an increased proportion of type ...
Tópico(s): Cardiomyopathy and Myosin Studies
2019 - Public Library of Science | PLoS ONE
Vanessa Haynes, Stacey N. Keenan, Jackie Bayliss, Erin M. Lloyd, Peter J. Meikle, Miranda D. Grounds, Matthew J. Watt,
... muscle lipidome using young adult dysferlin-deficient male BLAJ mice and age-matched C57Bl/6J WT mice. BLAJ mice had increased lean mass and reduced fat ... Skeletal muscle fatty acid metabolism was remodeled in BLAJ mice, characterized by a partitioning of fatty acids ... lipid classes examined in the skeletal muscle of BLAJ mice, including sphingolipids, phospholipids, cholesterol, and most glycerolipids ...
Tópico(s): Muscle metabolism and nutrition
2019 - Elsevier BV | Journal of Lipid Research
Florina Cudur, Maria Iliescu, Maria COMÅžA, Daniela Popescu, Cristian Cristea,
... at Reasearch Station for Viticulture and Enology (SCDVV)Blaj and aimed the pretability of varieties for quality ... wasdetermined in conditions of the varieties studied in Blaj wine center (2011 and 2012) on the two ... fertility parameters(pH, total nitrogen, phosphorus and potassium). Blaj vineyard has a long tradition, especially in thedirection ...
Tópico(s): Botanical Studies and Applications
2014 - AcademicPres | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture
A. Luis, Gabriela Fernández, Guisselle Arias-Bravo, Mario Castillo‐Ruiz, Rosalba Escamilla, María C. Brañes, Juan C. Sáez,
... recently demonstrated that myofibers of skeletal muscles from blAJ mice (an animal model of dysferlinopathies) express three ... functional hemichannels (HCs) in the sarcolemma. In symptomatic blAJ mice, we now show that eight-week treatment ... CK activity in serum. Myofibers of boldine-treated blAJ mice also showed strong dysferlin-like immunoreactivity. These ...
Tópico(s): Adipose Tissue and Metabolism
2020 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Morium Begam, Alyssa F. Collier, Amber L. Mueller, Renuka Roche, Sujay Galen, Joseph A. Roche,
B6.A-Dysfprmd/GeneJ (BLAJ) mice model human limb-girdle muscular dystrophy 2B (LGMD2B), which is linked to mutations in the dysferlin (DYSF) gene. We ... DTZ), reduces contraction-induced skeletal muscle damage, in BLAJ mice. We randomly assigned mice (N = 12; 3– ...
Tópico(s): Genetic Neurodegenerative Diseases
2018 - Wiley | Physiological Reports
Norifumi Urao, Rita E. Mirza, Ahlke Heydemann, Jesús Garcı́a, Timothy J. Koh,
... macrophage activity and disease progression in dysferlin deficient BlaJ mice, focusing on the early disease process. In 3 month-old BlaJ mice, muscle TSP-1 levels exhibited strong positive ... muscle degeneration, particularly early in disease progression in BlaJ mice. These data indicate that TSP-1 may ...
Tópico(s): Protease and Inhibitor Mechanisms
2016 - Elsevier BV | Neuromuscular Disorders
Elena S. Koltun, Meghan A. Rice, W. Clay Gustafson, David Wilds, Jingjing Jiang, Bianca J. Lee, Zhengping Wang, Stephanie Chang, Mike Flagella, Yunming Mu, Nuntana Dinglasan, Nicole Nasholm, James Evans, Yingyun Wang, Kyle J. Seamon, Yang Liu, Cristina Blaj, John E. Knox, Rebecca Freilich, Elsa Quintana, Jim Cregg, Alun Bermingham, Adrian L. Gill, Jacqueline AM Smith, Mallika Singh,
... Evans, Yingyun Wang, Kyle Seamon, Yang Liu, Cristina Blaj, John Knox, Rebecca Freilich, Elsa Quintana, Jim Cregg, ...
Tópico(s): Ubiquitin and proteasome pathways
2022 - American Association for Cancer Research | Cancer Research
Elena S. Koltun, Jim Cregg, Meghan A. Rice, Dan Whalen, Rebecca Freilich, Jingjing Jiang, Richard L. Hansen, Alun Bermingham, John E. Knox, Jay Dinglasan, Kyle J. Seamon, Cristina Blaj, Stephanie Chang, Yang Liu, Jun Huang, Kang-Jye Chou, Laura McDowell, Bianca J. Lee, David Wildes, Zhengping Wang, Mallika Singh, Adrian L. Gill, Jacqueline A.M. Smith,
... John E. Knox, Jay Dinglasan, Kyle Seamon, Cristina Blaj, Stephanie S. Chang, Yang Liu, Jun Huang, Kang- ...
Tópico(s): Biochemical and Molecular Research
2021 - American Association for Cancer Research | Cancer Research
Norifumi Urao, Rita E. Mirza, Thomas F. Corbiere, Zsuzsanna Hollander, Christoph H. Borchers, Timothy J. Koh,
... correlated with disease progression. We then crossed dysferlinopathic BlaJ mice with TSP-1 knockout mice and assessed ... an accessory role in modulating Mp activity in BlaJ mice in a gender, age and muscle-dependent ...
Tópico(s): Body Contouring and Surgery
2017 - Oxford University Press | Human Molecular Genetics
Christine Woischke, Cristina Blaj, Eva Marina Schmidt, Sebastian Lamprecht, Jutta Engel, Heiko Hermeking, Thomas Kirchner, David Horst,
// Christine Woischke 1, * , Cristina Blaj 1, * , Eva Marina Schmidt 1, * , Sebastian Lamprecht 1 , Jutta Engel 2 , Heiko Hermeking 1, 3, 4 , Thomas Kirchner 1, 3, ...
Tópico(s): Cancer-related Molecular Pathways
2016 - Impact Journals LLC | Oncotarget
Mirella Meregalli, Claire Navarro, Clementina Sitzia, Andrea Farini, Erica Montani, Nicolas Wein, P. Razini, Cyriaque Beley, Letizia Cassinelli, D. Parolini, M. Belicchi, Dario Parazzoli, Luis Garcı́a, Yvan Torrente,
... to express dysferlin after intramuscular transplantation into scid/blAJ dysferlin null mice. We thus extended these experiments ... engineered blood-derived CD133+ stem cells into scid/blAJ mice resulted in sufficient dysferlin expression to correct ...
Tópico(s): Virus-based gene therapy research
2013 - Wiley | FEBS Journal
Erin M. Lloyd, Gavin J. Pinniger, Miranda D. Grounds, Robyn M. Murphy,
... contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for ... wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice. Additionally, we compared the Ca2+ activation ... twitch myofibers from 3-month-old WT and BLAJ male mice. Differences were observed for some Ca2+ ... and glucose/glycogen metabolism-related protein levels between BLAJ soleus and EDL muscles (compared with WT) that ... the previously reported differences in function in these BLAJ muscles. Dysferlin deficiency did not impact glycogen content ...
Tópico(s): Adipose Tissue and Metabolism
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Marie Ménard, Christopher W.K. Chow, Kevin Chen, Cristina Blaj, Nataliya Tovbis Shifrin, Haley Courtney, Nicole Nasholm, Anna Pham, Alice Kumamoto, Swetha Ganesh, James Evans, Lindsey Lawrence, Brian Vonmelchert, Amy C. Kwok-Parkhill, Jingjing Jiang, John E. Knox, Jacqueline A.M. Smith, Elsa Quintana,
... Marie J. Menard, Christopher Chow, Kevin Chen, Cristina Blaj, Nataliya Tovbis Shifrin, Haley Courtney, Nicole M. Nasholm, ...
Tópico(s): Ubiquitin and proteasome pathways
2023 - American Association for Cancer Research | Cancer Research
N Hazumi, A Fuse, Keiji Matsuda, Terutaka Hashizume, M Sanada,
... derived from beta-lactamase-deficient P. aeruginosa PAO4089 (blaJ blaP). Complementation of the blaJ and blaP mutations conferred greater resistance to biapenem, ...
Tópico(s): Infections and bacterial resistance
1995 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Ioan Sabin Sopa, Dan Alexandru Szabo,
... failure of the CSM Volleyball team Volei Alba Blaj in the Final Four CEV Champions League for ...
Tópico(s): Sports Analytics and Performance
2019 - Mirton Publishing House, Timisoara | Timisoara Physical Education and Rehabilitation Journal
Maria Iliescu, Daniela Popescu, M. Comşa,
... theagricultural techniques applied. The study conducted at RSVO Blaj aimed to identify thedegree of maturation of rootstock ...
Tópico(s): Banana Cultivation and Research
2012 - AcademicPres | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture
Mihnea Miron, Mihaela Blaj, Irina Ristescu, G Iosep, Andrei-Nicolae Avădanei, Diana Gabriela Iosep, Radu Crișan-Dabija, A Ciocan, Mihaela Perţea, Carmen Manciuc, Ștefana Luca, Cristina Grigorescu, Mihaela Cătălina Luca,
Hospital-acquired pneumonia (HAP) and its subtype, ventilator-associated pneumonia (VAP), remain two significant causes of morbidity and mortality worldwide, despite the better understanding of pathophysiological mechanisms, etiology, risk factors, preventive methods (bundle of care principles) and supportive care. Prior detection of the risk factors combined with a clear clinical judgement based on clinical scores and dosage of different inflammatory biomarkers (procalcitonin, soluble triggering receptor ...
Tópico(s): Intensive Care Unit Cognitive Disorders
2024 - Multidisciplinary Digital Publishing Institute | Microorganisms
Matthew Holderfield, Bianca J. Lee, Jingjing Jiang, Aidan C.A. Tomlinson, Kyle J. Seamon, Alessia Mira, Enrico Patrucco, Grace Goodhart, Julien Dilly, Yevgeniy Gindin, Nuntana Dinglasan, Yingyun Wang, Lo Lai, Shurui Cai, Lingyan Jiang, Nicole Nasholm, Nataliya Tovbis Shifrin, Cristina Blaj, Harshit Shah, James Evans, Nilufar Montazer, Oliver Lai, Jue Shi, Ethan Ahler, Elsa Quintana, Stephanie Chang, Anthony Salvador, Abby Marquez, Jim Cregg, Yang Liu, Anthony N. Milin, Anqi Chen, Tamar Bar Ziv, Dylan Parsons, John E. Knox, Jennifer E. Klomp, Jennifer A. Roth, Matthew G. Rees, Melissa M. Ronan, Antonio Cuevas-Navarro, Feng Hu, Piro Lito, David Santamarı́a, Andrew J. Aguirre, Andrew M. Waters, Channing J. Der, Chiara Ambrogio, Zhengping Wang, Adrian L. Gill, Elena S. Koltun, Jacqueline A.M. Smith, David Wildes, Mallika Singh,
Abstract RAS oncogenes (collectively NRAS , HRAS and especially KRAS ) are among the most frequently mutated genes in cancer, with common driver mutations occurring at codons 12, 13 and 61 1 . Small molecule inhibitors of the KRAS(G12C) oncoprotein have demonstrated clinical efficacy in patients with multiple cancer types and have led to regulatory approvals for the treatment of non-small cell lung cancer 2,3 . Nevertheless, KRAS G12C mutations account for only around 15% of KRAS -mutated cancers 4,5 , and ...
Tópico(s): Melanoma and MAPK Pathways
2024 - Nature Portfolio | Nature
Jingjing Jiang, Lingyan Jiang, Benjamin J. Maldonato, Yingyun Wang, Matthew Holderfield, Ida Aronchik, Ian P. Winters, Zeena Salman, Cristina Blaj, Marie Ménard, Jens Brodbeck, Zhe Chen, Xing Wei, Michael Rosen, Yevgeniy Gindin, Bianca J. Lee, James W. Evans, Stephanie Chang, Zhican Wang, Kyle J. Seamon, Dylan Parsons, James Cregg, Abby Marquez, Aidan C.A. Tomlinson, Jason K. Yano, John E. Knox, Elsa Quintana, Andrew J. Aguirre, Kathryn C. Arbour, Abby Reed, W. Clay Gustafson, Adrian L. Gill, Elena S. Koltun, David Wildes, Jacqueline A.M. Smith, Zhengping Wang, Mallika Singh,
Abstract RAS-driven cancers comprise up to 30% of human cancers. RMC-6236 is a RAS(ON) multi-selective noncovalent inhibitor of the active, GTP-bound state of both mutant and wild-type variants of canonical RAS isoforms with broad therapeutic potential for the aforementioned unmet medical need. RMC-6236 exhibited potent anticancer activity across RAS-addicted cell lines, particularly those harboring mutations at codon 12 of KRAS. Notably, oral administration of RMC-6236 was tolerated in vivo and ...
Tópico(s): Cancer Genomics and Diagnostics
2024 - American Association for Cancer Research | Cancer Discovery
Lars D. Engstrom, Ruth Aranda, Laura Waters, Krystal Moya, Vickie Bowcut, Laura Vegar, David Trinh, Allan Hebbert, Christopher R. Smith, Svitlana Kulyk, J. David Lawson, Leo He, Laura D. Hover, Julio Fernandez-Banet, Jill Hallin, Darin Vanderpool, David M. Briere, Alice Blaj, Matthew A. Marx, Jordi Rodón, Michael Offin, Kathryn C. Arbour, Melissa L. Johnson, David J. Kwiatkowski, Pasi A. Jänne, Candace L. Haddox, Kyriakos P. Papadopoulos, Jason T. Henry, Konstantinos Leventakos, James G. Christensen, Ronald Shazer, Peter Olson,
Abstract Previous studies implicated protein arginine methyltransferase 5 (PRMT5) as a synthetic lethal target for MTAP-deleted (MTAP del) cancers; however, the pharmacologic characterization of small-molecule inhibitors that recapitulate the synthetic lethal phenotype has not been described. MRTX1719 selectively inhibited PRMT5 in the presence of MTA, which is elevated in MTAP del cancers, and inhibited PRMT5-dependent activity and cell viability with >70-fold selectivity in HCT116 MTAP del compared ...
Tópico(s): Epigenetics and DNA Methylation
2023 - American Association for Cancer Research | Cancer Discovery
Karina-Alexandra Cojocaru, Ionuț Luchian, Ancuța Goriuc, Lucian-Mihai Antoci, Cristian-Gabriel Ciobanu, Roxana Popescu, Cristiana-Elena Vlad, Mihaela Blaj, Liliana Foia,
Mitochondria are subcellular organelles involved in essential cellular functions, including cytosolic calcium regulation, cell apoptosis, and reactive oxygen species production. They are the site of important biochemical pathways, including the tricarboxylic acid cycle, parts of the ureagenesis cycle, or haem synthesis. Mitochondria are responsible for the majority of cellular ATP production through OXPHOS. Mitochondrial dysfunction has been associated with metabolic pathologies such as diabetes, ...
Tópico(s): Cancer, Hypoxia, and Metabolism
2023 - Multidisciplinary Digital Publishing Institute | Antioxidants
Natalie K. Wolf, Cristina Blaj, Lora K. Picton, Gail Snyder, Li Zhang, Christopher J. Nicolai, Chudi Ndubaku, Sarah M. McWhirter, K. Christopher García, David H. Raulet,
Cyclic dinucleotides (CDN) and Toll-like receptor (TLR) ligands mobilize antitumor responses by natural killer (NK) cells and T cells, potentially serving as complementary therapies to immune checkpoint therapy. In the clinic thus far, however, CDN therapy targeting stimulator of interferon genes (STING) protein has yielded mixed results, perhaps because it initiates responses potently but does not provide signals to sustain activation and proliferation of activated cytotoxic lymphocytes. To improve ...
Tópico(s): Viral Infections and Vectors
2022 - National Academy of Sciences | Proceedings of the National Academy of Sciences
A Vasilescu, E Târcoveanu, Cristian Lupaşcu, Mihaela Blaj, Corina Lupașcu-Ursulescu, C Bradea,
Abdominopelvic actinomycosis is a rare chronic or subacute bacterial infection caused by Actinomyces israelii, a Gram-positive anaerobic bacterium that normally colonizes the digestive and genital tracts, clinically presented as an inflammatory mass or abscess formation.We reviewed the medical records of the patients from our clinic with abdominopelvic actinomycosis who underwent surgery between 2002 and 2022. In this period, 28 cases (9 men and 19 women) were treated. The mean age was 43.36 years ...
Tópico(s): Sinusitis and nasal conditions
2022 - Multidisciplinary Digital Publishing Institute | Life
Bryan K Dunn, Michael Blaj, Jennifer Stahl, James Speicher, Carlos Anciano, Suzanne Hudson, Emily A. Kragel, Mark Bowling,
Background: Electromagnetic navigational bronchoscopy (ENB) has been shown to have variable diagnostic accuracy for the assessment of peripheral pulmonary nodules. This may be because of discrepancies between the preplanned computed tomography of chest target lesion location versus actual target location (computed tomography-to-body divergence), and the lack of a continuous navigational image. The ILLUMISITE (Medtronic, Minneapolis, MN) is a newly developed ENB platform that utilizes tomosynthesis, ...
Tópico(s): Atomic and Subatomic Physics Research
2022 - Lippincott Williams & Wilkins | Journal of Bronchology & Interventional Pulmonology
Veronica Sanda Chedea, Ana–Maria Drăgulinescu, Liliana Lucia TOMOIAGĂ, Cristina Bălăceanu, Maria Iliescu,
... is a red grape cultivar homologated at SCDVV Blaj, which also homologated 7 cultivars and 11 clones. ...
Tópico(s): Fermentation and Sensory Analysis
2021 - Multidisciplinary Digital Publishing Institute | Sustainability
Diana-Andreea Blaj, Mihaela Bălan, Brînduşa Alina Petre, Valeria Harabagiu, Cristian Peptu,
This paper aims to expand the range of matrix assisted laser desorption mass spectrometry analysis (MALDI MS) as a tool to establish the reaction kinetics for a particular case study: the synthesis of β-cyclodextrin-oligolactide (CDLA) derivatives through solution ring opening oligomerization (ROO) of d,l-lactide (LA) initiated by β-cyclodextrin (β-CD). Specifically, MALDI MS was used to estimate the molecular weight averages minute changes during the synthesis for a better understanding of the chemical ...
Tópico(s): Protein purification and stability
2021 - Elsevier BV | Polymer
Tim B. van Driel, S. Nelson, Rebecca Armenta, G. Blaj, Stephen Boo, Sébastien Boutet, D. Doering, A. Dragone, Philip Hart, G. Haller, C. Kenney, Maciej Kwaitowski, L. Manger, Mark E. McKelvey, Kaz Nakahara, M. Oriunno, Takahiro Sato, Matt Weaver,
The ePix10ka2M (ePix10k) is a new large area detector specifically developed for X-ray free-electron laser (XFEL) applications. The hybrid pixel detector was developed at SLAC to provide a hard X-ray area detector with a high dynamic range, running at the 120 Hz repetition rate of the Linac Coherent Light Source (LCLS). The ePix10k consists of 16 modules, each with 352 × 384 pixels of 100 µm × 100 µm distributed on four ASICs, resulting in a 2.16 megapixel detector, with a 16.5 cm × 16.5 cm active area and ∼ ...
Tópico(s): Advanced Electron Microscopy Techniques and Applications
2020 - Wiley | Journal of Synchrotron Radiation
Liliana Lucia TOMOIAGĂ, Veronica Sanda Chedea,
... Eutypa dieback and Crown gall) on the SCDVV Blaj homologated grapevine varieties, ‘Astra’, ‘Amurg’, ‘Brumariu’, ‘Blasius’ ‘Radames’, ‘ ...
Tópico(s): Yeasts and Rust Fungi Studies
2020 - AcademicPres | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture
Noemi Andor, Billy T. Lau, Claudia Catalanotti, Anuja Sathe, Matthew Kubit, Jiamin Chen, Cristina Blaj, Athena M. Cherry, Charles D. Bangs, Susan M. Grimes, Carlos J. Suarez, Hanlee P. Ji,
Abstract Cancer cell lines are not homogeneous nor are they static in their genetic state and biological properties. Genetic, transcriptional and phenotypic diversity within cell lines contributes to the lack of experimental reproducibility frequently observed in tissue-culture-based studies. While cancer cell line heterogeneity has been generally recognized, there are no studies which quantify the number of clones that coexist within cell lines and their distinguishing characteristics. We used ...
Tópico(s): Cancer Cells and Metastasis
2020 - Oxford University Press | NAR Genomics and Bioinformatics