Yu Fujita, Ali Khateb, Yan Li, Roberto Tinoco, Tongwu Zhang, Haggai Bar‐Yoseph, Miguel A. Tam, Yehuda Chowers, Edmond Sabo, Shiran Gerassy-Vainberg, Elina Starosvetsky, Brian P. James, Kevin M. Brown, Shai S. Shen-Orr, Linda M. Bradley, Philippe A. Tessier, Ze’ev A. Ronai,
Inflammatory bowel disease (IBD) is prevalent, but the mechanisms underlying disease development remain elusive. We identify a role for the E3 ubiquitin ligase RNF5 in IBD. Intestinal epithelial cells (IECs) express a high level of RNF5, while the colon of Rnf5-/- mice exhibits activated dendritic cells and intrinsic inflammation. Rnf5-/- mice exhibit severe acute colitis following dextran sodium sulfate (DSS) treatment. S100A8 is identified as an RNF5 substrate, resulting in S100A8 ubiquitination ...
Tópico(s): Neonatal Respiratory Health Research
2018 - Cell Press | Cell Reports
Mark E. Fuller, Brian J. Mailloux, Pengfei Zhang, Sheryl H. Streger, James A. Hall, Simon Vainberg, Andrew J. Beavis, William P. Johnson, T. C. Onstott, Mary F. DeFlaun,
This research was undertaken to evaluate staining with the fluorescent compound CFDA/SE (5-(and-6-)-carboxyfluorescein diacetate, succinimidyl ester) coupled with multiple cell detection methodologies as a means to monitor bacterial transport during field-scale experiments. Stained cells of Comamonas sp. strain DA001 were injected into a shallow, aerobic aquifer in Oyster, VA, USA. Groundwater samples analyzed in the laboratory using epifluorescence microscopy, flow cytometry, ferrographic separation, ...
Tópico(s): Underwater Vehicles and Communication Systems
2001 - Oxford University Press | FEMS Microbiology Ecology
Mark E. Fuller, Brian J. Mailloux, Sheryl H. Streger, James A. Hall, Pengfei Zhang, William P. Kovacik, Simon Vainberg, William P. Johnson, T. C. Onstott, Mary F. DeFlaun,
ABSTRACT Two differentially labeled bacterial strains were monitored in near-real time during two field-scale bacterial transport experiments in a shallow aquifer in July 2000 and July 2001. Comamonas sp. strain DA001 and Acidovorax sp. strain OY-107 were grown and labeled with the vital fluorescent stain TAMRA/SE (5 [and -6]-carboxytetramethylrhodamine, succinimidyl ester) or CFDA/SE (5 [and -6]-carboxyfluorescein diacetate, succinimidyl ester). Fluorescently labeled cells and a conservative bromide ...
Tópico(s): Fecal contamination and water quality
2004 - American Society for Microbiology | Applied and Environmental Microbiology