Lars Johan Larsson, E. P. Frisch, Karolina Törneke, Tua Lindblom, Ingemar Björk,
The proteinase, brinase (Mr approximately 35000), from Aspergillus oryzae, which has been used in therapeutic attempts as a thrombolytic agent in ... cleavage of the thioester bonds. The reaction with brinase is accompanied by a similar conformational change of ... shown by gradient gel electrophoresis and spectroscopic analyses. Brinase thus binds to alpha 2M in a similar ... complex formed at saturation of alpha 2M with brinase, the enzyme retains considerable proteolytic activity against macromolecular ... substrate. This finding indicates that the trapping of brinase by alpha 2M is less efficient than that ...
Tópico(s): Protease and Inhibitor Mechanisms
1988 - Elsevier BV | Thrombosis Research
F Lund, Stig Ekeström, E. P. Frisch, Fritz Magaard,
... by a series of i.v. infusions of brinase, a proteolytic enzyme from Aspergillus oryzae. The brinase inhibitor capacity in plasma was determined by the azocollagen technique. Dosage of brinase was calculated to retain a rest-inhibitor capacity ... instillation prior to a series of i.v. brinase infusions. Thirteen patients showed clinical improvement after brinase treatment. The condition of two patients remained unchanged, ... complete disobliteration after a series of i.v. brinase infusions. Bleeding complications associated with brinase treatment were ...
Tópico(s): Renal Diseases and Glomerulopathies
1975 - SAGE Publishing | Angiology
E. P. Frisch, M. Blombäck, Stig Ekeström, Hans Kiessling, F Lund, Fritz Magaard,
In 17 patients a total of 148 brinase infusions were given. All patients but two had anticoagulant treatment (dicoumarol or heparin) during the brinase series. Dosage of brinase was based on determination of brinase inhibitor capacity in plasma (azocollagen technique) prior to infusion of the enzyme. Iv infusion of brinase lowered inhibitors in plasma. Good correlation was found between expected lowering and measured inhibitor values after brinase infusion. The stipulated safety margin for brinase inhibitor ...
Tópico(s): Hemostasis and retained surgical items
1975 - SAGE Publishing | Angiology
Ph. Vanhove, Maria Benedetta Donati, H. Claeys, Raymond Verhaeghe, J. Vermylen,
Summary Brinase added to human plasma in vitro caused a decrease in fibrinogen concentration, positive paracoagulation tests and formation ... in the plasma samples obtained after infusion of Brinase was similar to that of the in vitro ... of fibrinogen was partially consumed by infusion of Brinase, but the turnover of plasminogen remained unaffected. Purified ... generation of proteolytic activity. These findings confirm that Brinase induces a proteolytic degradation of fibrinogen in plasma ...
Tópico(s): Erythropoietin and Anemia Treatment
1979 - Thieme Medical Publishers (Germany) | Thrombosis and Haemostasis
D.E. Fitzgerald, E. P. Frisch, James C. Milliken,
... therapeutic trial using placebo or the thrombolytic enzyme brinase was carried out in a group of patients ... receiving six intravenous infusions of either saline or brinase over a period of 2 weeks. Ankle blood ... the 3-month pre-observation period. After six brinase infusions, recanalization of 17 out of 27 obstructed ... in the placebo-treated patients. The differences between brinase and placebo treatment were statistically significant.
Tópico(s): Enzyme Production and Characterization
1979 - Taylor & Francis | Scandinavian Journal of Thoracic and Cardiovascular Surgery
... of thrombin and tranexamic acid (AMCA). Treatment with brinase (Protease I from Aspergillus oryzae) resulted in strongly ... pathogenesis of this pulmonary condition. The effect of brinase on fibrin elimination was not due to activation ...
Tópico(s): Blood Coagulation and Thrombosis Mechanisms
1976 - Elsevier BV | Thrombosis Research
... polymerization were digested by plasmin, trypsin, chymotrypsin and Brinase®. The degradation products in the supernatants were examined ... disappear on extended degradation by trypsin, chymotrypsin and Brinase. Plasmin sensitive, only E-antigenic fragments with some ... E after extended degradation by trypsin, chymotrypsin and Brinase show electrophoretic heterogeneity in CAIE and spur formation ...
Tópico(s): Enzyme Production and Characterization
1974 - Elsevier BV | Thrombosis Research
Harald Eie, Egil Amundsen, Johs. S. Rö,
... a wide catheter in the inferior vena cava. Brinase® (Astra, Södertälje, Sweden), a pro-teolytic enzyme from ... detected in all dogs which received 3 mg Brinase per kg b.w. or more. The enzyme ... toxic dosage to the dogs. The administration of Brinase was stopped, when Normotest values dropped to 50% ...
Tópico(s): Protease and Inhibitor Mechanisms
1972 - Taylor & Francis | Scandinavian Journal of Thoracic and Cardiovascular Surgery
Geraldine Flynn, T. J. Hackett, L. McHale, A. P. McHale,
... and delivery vehicles for these agents. The enzyme brinase, a fibrinolytic enzyme produced by Aspergillus oryzae, was ...
Tópico(s): Erythrocyte Function and Pathophysiology
1994 - Elsevier BV | Journal of Photochemistry and Photobiology B Biology
... surgery. Instead, induction of fibrinolysis by streptokinase or Brinase is suggested, because it increases the activity of ... significant. The action of streptokinase-induced plasmin and Brinase on lymphocytes is described.
Tópico(s): Cancer Treatment and Pharmacology
1975 - Wiley | Cancer
Preeti Holland, O Browne, R. D. Thornes,
... malignant disease. The addition of the proteolytic agent brinase (protease 1 obtained from Aspergillus oryzae) to the ... in the test. Slow intravenous infusion of either brinase or streptokinase into patients with malignant disease is ...
Tópico(s): Mast cells and histamine
1975 - Springer Nature | British Journal of Cancer
Harvey J. Freedman, Walter H. E. Roschlau,
... to standardized systemic administration of the fibrinolytic enzyme Brinase. The extent of toxicity (hypocoagulable blood, hypotension, hemoconcentration) ...
Tópico(s): Pharmacogenetics and Drug Metabolism
1970 - NRC Research Press | Canadian Journal of Physiology and Pharmacology
The rationale for clinical investigation of brinase may be summarized as follows:'Immediate treatment results: complete recanalization-blood flow restored to preclotting rate and volume; partial recanalization blood-flow ...
Tópico(s): Biological Research and Disease Studies
1972 - BMJ | Journal of Clinical Pathology
Noriaki Adachi, Shin’ichi Shoji, Shinichi Nakagawa, Chang‐Sung Koh, Naoyuki Tsukada, Nobuo Yanagisawa,
... between the 2 groups. The proteolytic effect of brinase, an enzyme from Aspergillus oryzae, on amyloid tissue ... was also evaluated. Amyloid fibrils were degraded by brinase, while the tissue structure remained fairly intact.
Tópico(s): Coagulation, Bradykinin, Polyphosphates, and Angioedema
1987 - Elsevier BV | Journal of the Neurological Sciences
... the thrombolytic enzyme protease I from Aspergillus oryzae (brinase) on the amyloid protein AA was investigated. The ... structure fairly intact. The in vivo effect of brinase on protein SAA was demonstrated in rabbits by ...
Tópico(s): Proteins in Food Systems
1982 - Wiley | Scandinavian Journal of Immunology
Brinase infusions in man cause, without excessive lowering of the inhibitor capacity, a slowing of the fibrin ... two major fragments. Both fragments carry crosslinking sites. Brinase infusion also causes positive ethanol gelation. Evidence for ...
Tópico(s): Erythrocyte Function and Pathophysiology
1975 - Thieme Medical Publishers (Germany) | Thrombosis and Haemostasis
Two methods for the assay of brinase inhibitors are compared. Neither provided a reliable means of brinase dose prediction. Overdosage can, however, be avoided by preinfusion inhibitor assay.
Tópico(s): Protein Kinase Regulation and GTPase Signaling
1975 - Thieme Medical Publishers (Germany) | Thrombosis and Haemostasis
Carmen Luisa Arocha-Piñango, Jonás Perales, Zoila Carvajal,
... differ form the one described for plasmin, trypsin, brinase and ochrase. The most striking difference being the ...
Tópico(s): Insect Resistance and Genetics
1981 - Thieme Medical Publishers (Germany) | Thrombosis and Haemostasis
... Proteolytic enzyme acting directly on fibrin (plasmin and brinase); (2) Drugs capable of enhancing the release of ...
Tópico(s): Peptidase Inhibition and Analysis
1979 - Elsevier BV | Progress in Cardiovascular Diseases
R. Verhaeghe, M Verstraete, John A. Schetz, Ph. Vanhove, R Suy, J. Vermylen,
Tópico(s): Chronic Kidney Disease and Diabetes
1979 - Springer Science+Business Media | European Journal of Clinical Pharmacology
Honor Smyth, Declan Farrell, R. D. Thornes, Norma Hiney,
Tópico(s): Venomous Animal Envenomation and Studies
1975 - Springer Science+Business Media | Irish Journal of Medical Science (1971 -)
Carlton E. Blackwood, J. Moret, Stephen Keller, Joshua Fierer, Ines Mandl,
... Shortly after injection with proteolytic enzymes, i.e., Brinase and pancreatic elastase, the inhibitor levels decreased markedly, ...
Tópico(s): Chronic Obstructive Pulmonary Disease (COPD) Research
1974 - Elsevier BV | Journal of Laboratory and Clinical Medicine
... and all of the microbial proteases investigated, including brinase gave a linear relationship between the logarithm of ...
Tópico(s): Enzyme Production and Characterization
1974 - Thieme Medical Publishers (Germany) | Thrombosis and Haemostasis
Tópico(s): Neurological Disorders and Treatments
1974 - SAGE Publishing | Angiology
Tópico(s): Enzyme Production and Characterization
1972 - BMJ | Journal of Clinical Pathology
... of the inhibitor EACA, and the lytic agent Brinase are described.
Tópico(s): Venous Thromboembolism Diagnosis and Management
1972 - Thieme Medical Publishers (Germany) | Thrombosis and Haemostasis
Honor Smyth, Eleanor Flahavan, R. D. Thornes,
Abstract Landschütz ascites tumour cells treated in vitro with Protease I showed no changes in intracellular K and Na levels when the cells were analysed directly at the end of the 60‐min incubation period. If, however, the cells were washed twice in the saline medium before analysis significant losses of K, apparently in exchange for Na, were found to take place. Controls incubated in the absence of Protease I and similarly treated showed no such K loss. Viability tests, using lissamine green, ...
Tópico(s): Peptidase Inhibition and Analysis
1971 - Wiley | International Journal of Cancer
... Activation Complex.- V. Novel Approaches.- 1. Defibrotide and Brinase.- VI. Side Effects and Complications.- VII. Clinical Considerations.- ...
Tópico(s): Plant-based Medicinal Research
1992 - Springer Science+Business Media | Handbook of experimental pharmacology
Tópico(s): Microbial Metabolites in Food Biotechnology
1977 - Elsevier BV | Thrombosis Research
The influence of brinase on fibrinogen levels, ethanol gelation test (EGT), fibrin content of lungs and kidneys (125I-fibrinogen) and brinase inhibitor capacity was investigated in the rat. The ... were either treated with i.v. infusions of brinase (4 mg/kg), tranexamic acid (AMCA) + brinase or heparin + AMCA + brinase. A control group was given i.v. saline, a reference group AMCA + thrombin. Brinase caused a decrease in fibrinogen levels and an ... deposition of fibrin was not observed. Infusion of brinase into rats with inhibited fibrinolytic system (AMCA), caused ...
Tópico(s): Chronic Kidney Disease and Diabetes
1982 - Karger Publishers | Pathophysiology of Haemostasis and Thrombosis