Produção Nacional
Revisado por pares
José Britto‐Júnior, David Halen Araújo Pinheiro, Alberto Fernando Oliveira Justo, Guilherme M. Figueiredo Murari, Rafael Campos, Fernanda Viviane Mariano, Valéria Barbosa de Souza, André Almeida Schenka, Fabíola Z. Mónica, Edson Antunes, Gilberto De Nucci,
Abstract Electrical field stimulation (EFS) induces contractions of both snake aorta and human umbilical cord vessels (HUCV) which were dependent on the presence of the endothelium. This study aimed to establish the nature of the mediator(s) responsible for EFS‐induced contractions in HUCV. Rings with or without endothelium from human umbilical artery (HUA) or vein (HUV) were mounted in organ bath chambers containing oxygenated, heated Krebs‐Henseleit's solution. Basal release of dopamine (DA), noradrenaline, ...
Tópico(s): Cardiac electrophysiology and arrhythmias
2020 - Wiley | Pharmacology Research & Perspectives
Produção Nacional
Revisado por pares
José Britto‐Júnior, Natalícia de Jesus Antunes, Rafael Campos, Mauro Sucupira, Gustavo Duarte Mendes, Fernanda Fantin Fernandes, Manoel Odorico de Moraes, Maria Elisabete Amaral de Moraes, Gilberto De Nucci,
Abstract This study presented for the first time the development and validation of a sensitive method for quantification of dopamine, noradrenaline, and adrenaline in Krebs–Henseleit solution by LC–tandem mass spectrometry. Aliquots of 2.0 mL calibrators, quality controls, and samples of Krebs–Henseleit solution incubated with tortoise's aortic ring for 30 min were extracted by solid‐phase extraction. Catecholamine separation was achieved on a 100 × 4.6 mm LiChrospher RP‐8 column and the quantification ...
Tópico(s): Analytical Chemistry and Sensors
2020 - Wiley | Biomedical Chromatography
Produção Nacional
Revisado por pares
José Britto‐Júnior, Antonio Tiago Lima, Vivian Fuguhara, Fabíola Z. Mónica, Edson Antunes, Gilberto De Nucci,
6-Nitrodopamine (6-ND) is released from rat isolated atria being 100 times more potent than noradrenaline and adrenaline, and 10,000 times more potent than dopamine as a positive chronotropic agent. The present study aimed to investigate the interactions of 6-ND with the classical catecholamines, phosphodiesterase (PDE)-3 and PDE4, and the protein kinase A in rat isolated atria. Atrial incubation with 1 pM of dopamine, noradrenaline, or adrenaline had no effect on atrial frequency. Similar results ...
Tópico(s): Nitric Oxide and Endothelin Effects
2023 - Springer Science+Business Media | Naunyn-Schmiedeberg s Archives of Pharmacology
Produção Nacional
Revisado por pares
José Britto‐Júnior, Luiz Ximenes, André Ribeiro, Adriano Fregonesi, Rafael Campos, Luiz Ricardo de Almeida Kiguti, Fabíola Z. Mónica, Edson Antunes, Gilberto De Nucci,
6-nitrodopamine (6-ND) is released from human umbilical cord vessels and modulates vascular reactivity by acting as a dopamine antagonist. Here we investigate whether 6-ND is released by the rat isolated vas deferens and its effect on this tissue. Dopamine, noradrenaline, adrenaline and 6-ND levels were quantified in rat isolated vas deferens by LC-MS-MS. Electric-field stimulation (EFS) and concentration-response curves to 6-ND, noradrenaline, dopamine and adrenaline were performed in the absence ...
Tópico(s): Ion channel regulation and function
2021 - Elsevier BV | European Journal of Pharmacology
Produção Nacional
Revisado por pares
Rafael Campos, David Halen Araújo Pinheiro, José Britto‐Júnior, Heleson Alves de Castro, Gustavo Duarte Mendes, Manoel Odorico de Moraes, Maria Elisabete Amaral de Moraes, Rodrigo Álvaro Brandão Lopes‐Martins, Natalícia de Jesus Antunes, Gilberto De Nucci,
Tópico(s): Environmental Toxicology and Ecotoxicology
2021 - Elsevier BV | Journal of Chromatography B
Produção Nacional
Revisado por pares
José Britto‐Júnior, André Ribeiro, Luiz Ximenes, Antonio Tiago Lima, Felipe Fernandes Jacintho, Adriano Fregonesi, Fabíola Z. Mónica, Edson Antunes, Gilberto De Nucci,
Tópico(s): Receptor Mechanisms and Signaling
2021 - Elsevier BV | European Journal of Pharmacology
Produção Nacional
Revisado por pares
José Britto‐Júnior, Weverton Castro Coelho Silva, Guilherme Figueiredo Murari, Charles Elliot Serpellone Nash, Fabíola Z. Mónica, Edson Antunes, Gilberto De Nucci,
Human umbilical cord vessels (HUCV) release dopamine and nitric oxide (NO). This study aims to verify whether HUCV release nitrocatecholamines such as 6-nitrodopamine (6-ND). Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to identify 6-ND release from HUCV rings incubated in Krebs-Henseileit's solution. Vascular reactivity of HUCV rings was tested (with and without endothelium integrity) by suspension of the rings in an organ bath under isometric tension and application ...
Tópico(s): Aortic Disease and Treatment Approaches
2021 - Elsevier BV | Life Sciences
Produção Nacional
Revisado por pares
José Britto‐Júnior, Walter Pinto da Silva‐Filho, Amanda Consulin Amorim, Rafael Campos, Manoel Odorico de Moraes, Maria Elisabete Amaral de Moraes, Adriano Fregonesi, Fabíola Z. Mónica, Edson Antunes, Gilberto De Nucci,
Rat isolated vas deferens releases 6-nitrodopamine (6-ND), and the spasmogenic activity of this novel catecholamine is significantly reduced by tricyclic compounds such as amitriptyline, desipramine, and carbamazepine and by antagonists of the α1 -adrenergic receptors such as doxazosin, tamsulosin, and prazosin.To investigate the liberation of 6-ND by human epididymal vas deferens (HEVDs) and its pharmacological actions.The in vitro liberation of 6-ND, dopamine, noradrenaline, and adrenaline from human ...
Tópico(s): Migraine and Headache Studies
2022 - Wiley | Andrology
Produção Nacional
Revisado por pares
José Britto‐Júnior, Mariana G. de Oliveira, Carolina dos Reis Gati, Rafael Campos, Manoel Odorico de Moraes, Maria Elisabete Amaral de Moraes, Fabíola Z. Mónica, Edson Antunes, Gilberto De Nucci,
6-Nitrodopamine (6-ND) is released by rat vas deferens and exerts a potent contractile response that is antagonized by tricyclic antidepressants and α1-, β1- and β1/β2-adrenoceptor antagonists. The release of 6-ND, noradrenaline, adrenaline and dopamine from rat isolated right atria was assessed by tandem mass spectrometry. The effects of the catecholamines were evaluated in both rat isolated right atria and in anaesthetized rats. 6-ND was the major catecholamine released from the isolated atria ...
Tópico(s): Renin-Angiotensin System Studies
2022 - Elsevier BV | Life Sciences
Produção Nacional
Revisado por pares
Antonio Tiago Lima, Eric Xavier dos Santos, José Britto‐Júnior, Valéria Barbosa de Souza, André Almeida Schenka, Rafael Campos, Manoel Odorico de Moraes, Maria Elisabete Amaral de Moraes, Edson Antunes, Gilberto De Nucci,
6-Nitrodopamine (6-ND) is a novel catecholamine that is released from human umbilical cord vessels and Chelonoidis carbonaria aortic rings. The synthesis/release of 6-ND is inhibited by either pre-incubation of the vessels with the nitric oxide (NO) synthase inhibitor L-NAME or by mechanical removal of the endothelium. 6-ND causes powerful vasorelaxation, acting as a potent and selective dopamine D2-like receptor antagonist. Basal release of 6-ND from Panterophis guttatus endothelium intact and denuded ...
Tópico(s): Neuropeptides and Animal Physiology
2022 - Elsevier BV | Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology
Produção Nacional
Revisado por pares
Gilberto Quirino Júnior, José Britto‐Júnior, Tainah Babadopulos Magalhaes, Rafael Campos, Kudzai Nyamkondiwa, Kameron L. Klugh, Larryn W. Peterson, Angela Corvino, Rosa Sparaco, Francesco Frecentese, Giuseppe Caliendo, Gilberto De Nucci,
This study presents the validation of a sensitive method for the determination of 6-nitrodopa, 6-nitrodopamine, 6-nitroadrenaline and 6-cyanodopamine in Krebs-Henseleit solution by LC-MS/MS with ESI+ . HRMS was used to precisely characterize the structures of the fragment ions. The method was applied to investigate the catecholamine basal release from rabbit isolated atria and ventricles. The atria and ventricles were suspended separately in a 5 ml organ bath containing Krebs-Henseleit solution with ...
Tópico(s): Analytical Methods in Pharmaceuticals
2023 - Wiley | Biomedical Chromatography
Produção Nacional
Revisado por pares
José Britto‐Júnior, Antonio Tiago Lima, J.S. Santos-Xavier, Pedro Luiz Gonzalez, Fabíola Z. Mónica, Rafael Campos, Valéria Barbosa de Souza, André Almeida Schenka, Edson Antunes, Gilberto De Nucci,
6-Nitrodopamine is a novel catecholamine released by vascular tissues, heart, and vas deferens. The aim of this study was to investigate whether 6-nitrodopamine is released from the thoracic aorta and pulmonary artery rings of marmosets (Callithrix spp.) and to evaluate the relaxing and anti-contractile actions of this catecholamine. Release of 6-nitrodopamine, dopamine, noradrenaline, and adrenaline was assessed by liquid chromatography with tandem mass spectrometry (LC-MS/MS). The relaxations induced ...
Tópico(s): Ion channel regulation and function
2023 - Associação Brasileira de Divulgação Científica | Brazilian Journal of Medical and Biological Research
Produção Nacional
Revisado por pares
José Britto‐Júnior, Felipe Fernandes Jacintho, Guilherme M. Figueiredo Murari, Rafael Campos, Ronílson Agnaldo Moreno, Edson Antunes, Fabíola Z. Mónica, Gilberto De Nucci,
Electrical field stimulation (EFS) has been used for decades in classical pharmacological preparations in order to characterize the mediators released by neural endings involved in smooth muscle contraction or relaxation. Since most of the human umbilical cord has no innervation, EFS has never been used in this preparation. This study aimed to investigate the effect of EFS on vascular responsiveness from human umbilical cord. Segments of the human umbilical cord were obtained from normotensive parturients ...
Tópico(s): Cardiac electrophysiology and arrhythmias
2020 - Elsevier BV | Life Sciences
Produção Nacional
Revisado por pares
José Britto‐Júnior, Rafael Campos, Matheus Peixoto, Antonio Tiago Lima, Felipe Fernandes Jacintho, Fabíola Z. Mónica, Ronílson Agnaldo Moreno, Edson Antunes, Gilberto De Nucci,
Chelonoidis carbonaria aortic rings present endothelium-derived release of dopamine, noradrenaline, adrenaline and 6-nitrodopamine (6-ND). Here it was investigated whether 6-ND release is coupled to nitric oxide (NO) synthesis and its action on the vascular smooth muscle reactivity. Basal release of 6-ND from aortic rings in the absence and presence of the NO synthesis inhibitor L-NAME was quantified by LC-MS-MS. Aortic rings were suspended vertically between two metal hooks in 10-mL organ baths containing ...
Tópico(s): Zebrafish Biomedical Research Applications
2022 - Elsevier BV | Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology
Produção Nacional
Revisado por pares
José Britto‐Júnior, Gustavo L. Pereira do Prado, Silvana Chiavegatto, Fernando Q. Cunha, Manoel Odorico de Moraes, Maria Elisabete Amaral de Moraes, Fabíola Z. Mónica, Edson Antunes, Gilberto De Nucci,
6-nitrodopamine (6-ND) is released from rat isolated atria, where it acts as a potent positive chronotropic agent. The release of 6-ND from rat isolated atria and ventricles is significantly reduced when pre-incubated with l-NAME, and the release was not affected by tetrodotoxin pre-treatment, indicating that in the heart, the origin of 6-ND is not neurogenic. Since l-NAME inhibits all three isoforms of NO synthase, it was investigated the basal release of 6-ND from isolated atria and ventricles ...
Tópico(s): Neurotransmitter Receptor Influence on Behavior
2023 - Elsevier BV | Nitric Oxide
Produção Nacional
Revisado por pares
José Britto‐Júnior, Lincoln Medeiros-Teixeira, Antonio Tiago Lima, Letícia Costa Dassow, Rodrigo Álvaro Brandão Lopes‐Martins, Rafael Campos, Manoel Odorico de Moraes, Maria Elisabete Amaral de Moraes, Edson Antunes, Gilberto De Nucci,
Background: 6-nitrodopamine released from rat isolated atria exerts positive chronotropic action, being more potent than noradrenaline, adrenaline, and dopamine. Here, we determined whether 6-nitrodopamine is released from rat isolated ventricles (RIV) and modulates heart inotropism. Methods: Catecholamines released from RIV were quantified by LC-MS/MS and their effects on heart inotropism were evaluated by measuring left ventricular developed pressure (LVDP) in Langendorff’s preparation. Results: ...
Tópico(s): Cardiac Ischemia and Reperfusion
2023 - Multidisciplinary Digital Publishing Institute | Life
Produção Nacional
Revisado por pares
Rafael Campos, Felipe Fernandes Jacintho, José Britto‐Júnior, Fabíola Z. Mónica, Alberto Fernando Oliveira Justo, André Sampaio Pupo, Ronílson Agnaldo Moreno, Valéria Barbosa de Souza, André Almeida Schenka, Edson Antunes, Gilberto De Nucci,
The role of endothelium in the electrical-field stimulation (EFS)-induced contractions of Chelonoidis carbonaria aorta was investigated. Contractions were evaluated in the presence and absence of L-NAME (100 μM), tetrodotoxin (1 μM), phentolamine (10 and 100 μM), phenoxybenzamine (1 and 10 μM), prazosin (100 μM), idazoxan (100 μM), atropine (10 μM), D-tubocurarine (10 μM) or indomethacin (10 μM). EFS-induced contraction was also carried out in endothelium-denuded rings. EFS-induced contraction was investigated ...
Tópico(s): Ion channel regulation and function
2020 - Elsevier BV | Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology
Luiz Ximenes, José Britto‐Júnior, Walter Silva-Filho, Felipe Fernandes Jacintho, Milton Cuppoloni, Adriano Fregonesi, Gilberto De Nucci,
... AND MODULATES ITS CONTRACTILITY Luiz Eduardo Ximenes, José Britto-Júnior, Walter Silva-Filho, Felipe Jacintho, Milton Cuppoloni, Adriano ... More articles by this author , José Britto-JúniorJosé Britto-Júnior More articles by this author , Walter Silva-FilhoWalter ... Eduardo Ximenes More articles by this author José Britto-Júnior More articles by this author Walter Silva-Filho ...
Tópico(s): Tissue Engineering and Regenerative Medicine
2021 - Lippincott Williams & Wilkins | The Journal of Urology
Produção Nacional
Revisado por pares
José Britto‐Júnior, Antonio Tiago Lima, Rafael Campos, Alexandre Asturiano Gião, Gustavo Duarte Mendes, Luiza P. Ferreira-Novaes Silva, Adriano Fregonesi, André Sampaio Pupo, Edson Antunes, Gilberto De Nucci,
6-Nitrodopamine (6-ND) is a novel endogenous catecholamine that is released from the rat isolated vas deferens, and has been characterized as a major modulator of the contractility of rat isolated epididymal vas deferens (RIEVD). Drugs such as tricyclic antidepressants, α1 and β1β2 adrenoceptor blockers, act as selective antagonists of the 6-ND receptor in the RIEVD. In the rat isolated atria, 6-ND has a potent positive chronotropic action and causes remarkable potentiation of the positive chronotropic ...
Tópico(s): Renin-Angiotensin System Studies
2023 - Springer Science+Business Media | Naunyn-Schmiedeberg s Archives of Pharmacology
Produção Nacional
Revisado por pares
Antonio Tiago Lima, Amanda Consulin Amorim, José Britto‐Júnior, Raquel Rios Campitelli, Adriano Fregonesi, Fabíola Z. Mónica, Edson Antunes, Gilberto De Nucci,
6-Nitrodopamine (6-ND) is an endogenous modulator of the contractility in the rat isolated epididymal vas deferens (RIEVD) and considered to be the main peripheral mediator of the emission process. Use of selective and unselective β-adrenergic receptor antagonists has been associated with ejaculatory failure. Here, the effects of selective β1- and β1/β2-adrenergic receptor antagonists on RIEVD contractions induced by 6-ND, dopamine, noradrenaline, adrenaline, and electric-field stimulation (EFS) were ...
Tópico(s): Neuroendocrine regulation and behavior
2022 - Springer Science+Business Media | Naunyn-Schmiedeberg s Archives of Pharmacology
Produção Nacional
Revisado por pares
Édison de Britto Rangel Júnior, Helga Loos,
Pessoas com TDAH parecem ter funcionamento neurológico peculiar. Sua hiperatividade cerebral, acompanhada eventualmente de hiperatividade física, causa instabilidade na capacidade atencional, gerando dificuldades no ajustamento à sistemática escolar tradicional. Entretanto, mostram-se sensíveis e criativas, até geniais. Este estudo objetivou investigar as percepções de adolescentes e jovens adultos com diagnóstico de TDAH acerca do papel da escola em seu desenvolvimento psicossocial, principalmente ...
Tópico(s): Education Pedagogy and Practices
2011 - UNIVERSIDADE DE SÃO PAULO | Paidéia (Ribeirão Preto)
Produção Nacional
Revisado por pares
José Britto‐Júnior, Felipe Fernandes Jacintho, Rafael Campos, David Halen Araújo Pinheiro, Guilherme M. Figueiredo Murari, Valéria Barbosa de Souza, André Almeida Schenka, Fabíola Z. Mónica, Ronílson Agnaldo Moreno, Edson Antunes, Gilberto De Nucci,
The contractions of Chelonoidis carbonaria aortic rings induced by electrical field stimulation (EFS) are not inhibited by blockade of the voltage-gated sodium channels by tetrodotoxin but almost abolished by the α1/α2-adrenoceptor antagonist phentolamine. The objective of this study was to identify the mediator(s) responsible for the EFS-induced contractions of Chelonoidis carbonaria aortic rings. Each ring was suspended between two wire hooks and mounted in isolated 10 mL organ chambers filled ...
Tópico(s): Ion channel regulation and function
2020 - The Company of Biologists | Biology Open
Produção Nacional
Revisado por pares
Luis Frederico Gerbase de Oliveira, José Britto‐Júnior, Antonio Tiago Lima, Manoel Odorico de Moraes, Maria Elisabete Amaral de Moraes, Valéria Barbosa de Souza, André Almeida Schenka, Fabíola Z. Mónica, Gilberto De Nucci,
6-Nitrodopamine (6-ND) is a novel catecholamine that is released from human umbilical cord vessels, and it causes vascular relaxation by acting as a dopamine D2-receptor antagonist. Here it was investigated whether human peripheral vessels obtained from patients who have undergone surgery for leg amputation release 6-ND, and its action in these tissues. Popliteal artery and vein strips present basal release of 6-ND, as measured by liquid chromatography coupled to tandem mass spectrometry. The release ...
Tópico(s): Pain Mechanisms and Treatments
2023 - Elsevier BV | Life Sciences
... 1007/s10101-015-0170-0José Alexandre da Silva Júnior, Dalson Britto Figueiredo Filho, Marolas ou tsunamis?: o impacto das ...
Tópico(s): Political Influence and Corporate Strategies
1978 - University of Chicago Press | The Journal of Politics
Produção Nacional
Revisado por pares
Fabrício Eneas Diniz de Figueiredo, Laila Fernandes Lima, Ludmila Smith de Jesus Oliveira, Maria Amália Gonzaga Ribeiro, Marcos Britto Corrêa, Manoel Brito-Júnior, André Luis Faria‐e‐Silva,
Tópico(s): Dental Anxiety and Anesthesia Techniques
2019 - Springer Nature | Clinical Oral Investigations
Produção Nacional
Revisado por pares
José Britto‐Júnior, Edson Hiroshi Salgado Uramoto, Antonio Tiago Lima, Luiz Fernando Ribeiro, Valéria Barbosa de Souza, André Almeida Schenka, José Cássio de Almeida Magalhães, Edson Antunes, Adriano Fregonesi, Gilberto De Nucci,
To evaluate the basal release of 6-nitrodopamine (6-ND) from human isolated seminal vesicles (HISV) and to characterize its action and origin. Left HISV obtained from patients undergoing prostatectomy surgery was suspended in a 3-mL organ bath containing warmed (37 °C) and gassed (95%O2:5%CO2) Krebs-Henseleit's solution (KHS) with ascorbic acid. An aliquot of 2 mL of the supernatant was used to quantify catecholamines by LC-MS/MS. For functional studies, concentration-responses curves to catecholamines ...
Tópico(s): Pain Mechanisms and Treatments
2024 - Elsevier BV | Life Sciences
Produção Nacional
Revisado por pares
José Britto‐Júnior, Samuel Goulart Nacário Silva, Antonio Tiago Lima, Vivian Fuguhara, Larissa Bueno Andrade, Gustavo Duarte Mendes, Larryn W. Peterson, Silvana Chiavegatto, Edson Antunes, Gilberto De Nucci,
6-Nitrodopamine (6-ND) is released from rat and human vas deferens and is considered a major mediator of both tissues contractility. The contractions induced by 6-ND are selectively blocked by both tricyclic antidepressants and α1-adrenoceptor antagonists. Endothelial nitric oxide synthase (eNOS) is the major isoform responsible for 6-ND release in mouse isolated heart, however the origin of 6-ND in the vas deferens is unknown. Here it was investigated by LC-MS/MS the basal release of 6-ND from isolated ...
Tópico(s): Ion channel regulation and function
2023 - Elsevier BV | Nitric Oxide
... career researchers promote themselves alongside their papers. José Britto-Júnior is first author on ‘The basal release of ...
Tópico(s): Nitric Oxide and Endothelin Effects
2021 - The Company of Biologists | Biology Open
Produção Nacional
Revisado por pares
Guilherme de Marco Antonello, Ricardo Torres do Couto, Caroline Comis Giongo, Marcos Britto Corrêa, Otacílio Luiz Chagas Júnior, Carmen Helena Jacques Lemes,
Platelet-rich plasma (PRP) is rich in growth factors, target-specific polypeptides that play a role in cell proliferation and differentiation and can thus encourage wound repair. This study sought to assess the effects of PRP on new bone formation in a sample of 25 patients with clinical indications for extraction of all four impacted third molars with similar orientation, depth, and root morphology. Immediately after extraction, sockets on one side received PRP, whereas those on the other side (control) ...
Tópico(s): Dental Radiography and Imaging
2013 - Elsevier BV | Journal of Cranio-Maxillofacial Surgery
Produção Nacional
Revisado por pares
Laura Vargas-Valencia, Felipe Britto Azevedo Schneider, Arnaldo Leal‐Junior, Pablo Eduardo Caicedo Rodríguez, Wilson Sierra, L. E. Rodríguez-Cheu, Teodiano Bastos-Filho, Anselmo Frizera,
The knee flexion-extension angle is an important variable to be monitored in various clinical scenarios, for example, during physical rehabilitation assessment. The purpose of this work is to develop and validate a sensor fusion system based on a knee sleeve for monitoring of physical therapy. The system consists of merging data from two inertial measurement units (IMUs) and an intensity-variation based Polymer Optical Fiber (POF) curvature sensor using a quaternion-based Multiplicative Extended Kalman ...
Tópico(s): Inertial Sensor and Navigation
2020 - Institute of Electrical and Electronics Engineers | IEEE Journal of Biomedical and Health Informatics