Produção Nacional
Revisado por pares
Clóvis A. Silva, Norma T. Foss,
... Tumor Necrosis Factor in Leprosy Patients Get access Célio L. Silva, Célio L. Silva Department of Parasitology, Microbiology, and Immunology, School of ... Brazil Please address requests for reprints to Dr. Célio L. Silva, Departamento de Microbiologia, Faculdade de Medicina de Ribeirão ...
Tópico(s): Ocular Diseases and Behçet’s Syndrome
1989 - Oxford University Press | The Journal of Infectious Diseases
Célio Silva, Heinz Troxler, Silke Durka, Jaroslav Chládek, Johannes H. Wildhaber, Felix H. Sennhauser, Claus W. Heizmann, Alexander Mœller,
3-Nitrotyrosine (3-NT) is considered as a marker of oxidative stress, which occurs during inflammation. Since 3-NT levels in exhaled breath condensate (EBC) are very low, we applied a specific and sensitive gas chromatography-negative ion chemical ionization-mass spectrometry (GC-NICI-MS) method and high performance liquid chromatography (HPLC) with electrochemical detection for the analysis of free 3-NT in EBC. A total of 42 children (aged 5-17 years) were enrolled in this study, including children ...
Tópico(s): Cystic Fibrosis Research Advances
2006 - Elsevier BV | Nitric Oxide
Produção Nacional
Revisado por pares
Anamélia Lorenzetti Bocca, M F Silva, Célio Lopes Silva, Fernando Q. Cunha, F. Figueiredo,
C57B1/6 isogenic mice infected with Paracoccidioides brasiliensis strains showed a disruption in the expression of Ia antigen. Expression slowly decreased during the course of the infection with a slight variation dependent on the route of inoculation and the fungal strain used, but production of interferon-gamma and tumor necrosis factor-alpha were observed. Suppression of Ia antigen expression and depression of the immunoproliferative responses of spleen cells were strongly correlated with nitric ...
Tópico(s): Toxoplasma gondii Research Studies
1999 - American Society of Tropical Medicine and Hygiene | American Journal of Tropical Medicine and Hygiene
Produção Nacional
Revisado por pares
Eliane Esteves, Bruna Bizzarro, Francisco B. Costa, Alejandro Ramírez‐Hernández, Ana Paula Ferranti Peti, Allan Henrique Depieri Cataneo, Pryscilla Fanini Wowk, Rodolfo Pessato Timóteo, Marcelo B. Labruna, Pedro Ismael da Silva, Célio Lopes Silva, Lúcia Helena Faccioli, Andréa Cristina Fogaça, Carlos A. Sorgi, Anderson Sá‐Nunes,
Amblyomma sculptum is an important vector of Rickettsia rickettsii, causative agent of Rocky Mountain spotted fever and the most lethal tick-borne pathogen affecting humans. To feed on the vertebrate host's blood, A. sculptum secretes a salivary mixture, which may interact with skin resident dendritic cells (DCs) and modulate their function. The present work was aimed at depicting the A. sculptum saliva-host DC network and the biochemical nature of the immunomodulatory component(s) involved in this ...
Tópico(s): Mosquito-borne diseases and control
2019 - Frontiers Media | Frontiers in Immunology
Produção Nacional
Revisado por pares
Júlio CC Lorenzi, Ana Paula Fávaro Trombone, Carolina D. Rocha, Luciana Previato de Almeida, Ricardo L Lousada, Thiago Malardo, Isabela C Fontoura, R. A. M. Rossetti, Ana Flávia Gembre, Aristóbolo M. Silva, Célio Lopes Silva, Arlete Aparecida Martins Coelho‐Castelo,
Abstract Background mRNAs are highly versatile, non-toxic molecules that are easy to produce and store, which can allow transient protein expression in all cell types. The safety aspects of mRNA-based treatments in gene therapy make this molecule one of the most promising active components of therapeutic or prophylactic methods. The use of mRNA as strategy for the stimulation of the immune system has been used mainly in current strategies for the cancer treatment but until now no one tested this ...
Tópico(s): Immune Response and Inflammation
2010 - BioMed Central | BMC Biotechnology
Produção Nacional
Revisado por pares
Priscilla Aparecida Tártari Pereira, Claudia S. Bitencourt, Mouzarllem Barros Reis, Fabiani Gai Frantz, Carlos A. Sorgi, Camila Oliveira Silva Souza, Célio Lopes Silva, Luiz Gustavo Gardinassi, Lúcia Helena Faccioli,
Tópico(s): Glycosylation and Glycoproteins Research
2019 - Springer Science+Business Media | Inflammation Research
Produção Nacional
Revisado por pares
Nildimar Alves Honório, Rita Maria Ribeiro Nogueira, Cláudia Torres Codeço, Marília Sá Carvalho, Oswaldo Gonçalves Cruz, Mônica de Avelar Figueirêdo Mafra Magalhães, Josélio Maria Galvão de Araújo, Eliane Saraiva Machado de Araújo, Marcelo Quintela Gomes, Luciane Silva Pinheiro, Célio da Silva Pinel, Ricardo Lourenço‐de‐Oliveira,
Background Rio de Janeiro, Brazil, experienced a severe dengue fever epidemic in 2008. This was the worst epidemic ever, characterized by a sharp increase in case-fatality rate, mainly among younger individuals. A combination of factors, such as climate, mosquito abundance, buildup of the susceptible population, or viral evolution, could explain the severity of this epidemic. The main objective of this study is to model the spatial patterns of dengue seroprevalence in three neighborhoods with different ...
Tópico(s): COVID-19 epidemiological studies
2009 - Public Library of Science | PLoS neglected tropical diseases
Produção Nacional
Revisado por pares
Carlos R. Zárate-Bladés, Vânia Luiza Deperon Bonato, Eduardo L. V. Silveira, Marina Oliveira e Paula, Cristina M. Junta, Paula Sandrin‐Garcia, Ana Lúcia Fachin, Stephano S. Mello, Renato S. Cardoso, Fabio Cícero de Sá Galetti, Arlete Aparecida Martins Coelho‐Castelo, Simone G. Ramos, Eduardo Antônio Donadi, Elza Tiemi Sakamoto‐Hojo, Geraldo Aleixo Silva Passos, Célio Lopes Silva,
The continued increase in tuberculosis (TB) rates and the appearance of extremely resistant Mycobacterium tuberculosis strains (XDR-TB) worldwide are some of the great problems of public health. In this context, DNA immunotherapy has been proposed as an effective alternative that could circumvent the limitations of conventional drugs. Nonetheless, the molecular events underlying these therapeutic effects are poorly understood.We characterized the transcriptional signature of lungs from mice infected ...
Tópico(s): interferon and immune responses
2008 - Wiley | The Journal of Gene Medicine
Produção Nacional
Revisado por pares
Rogério Silva Rosada, Célio Lopes Silva, Maria Helena Andrade Santana, Clóvis R. Nakaie, Lucimara Gaziola de la Torre,
We report the effects of a synthetic peptide designed to act as a nuclear localization signal on the treatment of tuberculosis. The peptide contains 21 amino acid residues with the following specific domains: nuclear localization signal from SV 40T, cationic shuttle sequence, and cysteamide group at the C-terminus. The peptide was complexed with the plasmid DNAhsp65 and incorporated into cationic liposomes, forming a pseudo-ternary complex. The same cationic liposomes, composed of egg chicken l- ...
Tópico(s): Immunotherapy and Immune Responses
2011 - Elsevier BV | Journal of Colloid and Interface Science
Produção Nacional
Revisado por pares
Fabíola Cristina Ribeiro Zucchi, Adriana Pelegrini-da-Silva, Luciano Neder, Célio Lopes Silva, Ana Maria Crous Tsanaclis, Osvaldo Massaiti Takayanagui,
Central nervous system (CNS) tuberculosis (TB) is the most severe form of TB, characterized morphologically by brain granulomas and tuberculous meningitis (TBM). Experimental strategies for the study of the host–pathogen interaction through the analysis of granulomas and its intrinsic molecular mechanisms could provide new insights into the neuropathology of TB. To verify whether cerebellar mycobacterial infection induces the main features of the disease in human CNS and better understand the physiological ...
Tópico(s): Bacterial Infections and Vaccines
2012 - Elsevier BV | Journal of Neuroscience Methods
Produção Nacional
Revisado por pares
Diana Bridon da Graça Sgarbi, Antônio Jorge Ribeiro da Silva, Iracilda Zeppone Carlos, Célio Lopes Silva, Jayme Angluster, Celuta S. Alviano,
Tópico(s): Phytochemical compounds biological activities
1997 - Springer Science+Business Media | Mycopathologia
Produção Nacional
Revisado por pares
Roberto G. S. Berlinck, Eduardo Hajdu, Rosana Moreira da Rocha, Jaine Honorata Hortolan Luiz, Isara L. C. Hernández, Mirna Helena Regali Seleghim, Ana Cláudia Granato, Érika Virgínia Raphael de Almeida, Cecília Veronica Núñez, Guilherme Ramos da Silva Muricy, Solange Peixinho, Cláudia Pessoa, Manoel Odorico de Moraes, Bruno C. Cavalcanti, Gislene G. F. Nascimento, Otávio Henrique Thiemann, Márcio Luís Andrade e Silva, Ana Olı́via de Souza, Célio Lopes Silva, Paulo Roberto Regazi Minarini,
Brazil is blessed with a great biodiversity, which constitutes one of the most important sources of biologically active compounds, even if it has been largely underexplored. As is the case of the Amazon and Atlantic rainforests, the Brazilian marine fauna remains practically unexplored in the search for new biologically active natural products. Considering that marine organisms have been shown to be one of the most promising sources of new bioactive compounds for the treatment of different human ...
Tópico(s): Biotechnology and Related Fields
2004 - American Chemical Society | Journal of Natural Products
Produção Nacional
Revisado por pares
Mariane Cajubá de Britto Lira Nogueira, Marigilson P. Siqueira-Moura, Hercília M. L. Rolim-Santos, Fábio C. S. Galetti, Andreza Ribeiro Simioni, Noêmia Pereira da Silva Santos, Eryvaldo Sócrates Tabosa do Egito, Célio Lopes Silva, Antônio Cláudio Tedesco, Nereide Stela Santos-Magalhães,
The cellular uptake and antimycobacterial activity of usnic acid (UA) and usnic acid-loaded liposomes (UA-LIPOs) were assessed on J774 macrophages. The minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) of UA and UA-LIPO against Mycobacterium tuberculosis were determined. Concentrations required to inhibit 50% of cell proliferation (IC50) were 22.5 (±0.60) and 12.5 (±0.26) μg/ml, for UA and UA-LIPO, respectively. The MICs of UA and UA-LIPO were 6.5 and 5.8 μg/mL, respectively. ...
Tópico(s): Mycorrhizal Fungi and Plant Interactions
2009 - Taylor & Francis | Journal of Liposome Research
Produção Nacional
Revisado por pares
Camila Guerra Martinez, Daniel Zamith‐Miranda, Marcia Gracindo da Silva, Karla Consort Ribeiro, Izaíra Trincani Brandão, Célio Lopes Silva, Bruno L. Diaz, Maria Bellio, Pedro M. Persechini, Eleonora Kurtenbach,
Abstract Autoantibodies against the M 2 receptors (M 2 AChR) have been associated with Dilated Cardiomyopathy (DCM). In the heart, P2×7 receptors influence electrical conduction, coronary circulation and response to ischemia. They can also trigger pro-inflammatory responses and the development of neurological, cardiac and renal disorders. Here, P2×7 −/− mice displayed an increased heart rate and ST segment depression, but similar exercise performance when compared to wild type (WT) animals. After immunization ...
Tópico(s): Immune Cell Function and Interaction
2015 - Nature Portfolio | Scientific Reports
Produção Nacional
Revisado por pares
Patrícia A. Assis, Milena S. Espíndola, Francisco Wanderley Garcia Paula‐Silva, Wendy Martin Rios, Priscilla Aparecida Tártari Pereira, Sylvia Cardoso Leão, Célio Lopes Silva, Lúcia Helena Faccioli,
Phospholipases C (PLCs) are virulence factors found in several bacteria. In Mycobacterium tuberculosis (Mtb) they exhibit cytotoxic effects on macrophages, but the mechanisms involved in PLC-induced cell death are not fully understood. It has been reported that induction of cell necrosis by virulent Mtb is coordinated by subversion of PGE2, an essential factor in cell membrane protection. Using two Mtb clinical isolates carrying genetic variations in PLC genes, we show that the isolate 97-1505, which ...
Tópico(s): Pneumonia and Respiratory Infections
2014 - BioMed Central | BMC Microbiology
Produção Nacional
Revisado por pares
Jaine Honorata Hortolan Luiz, Mirna Helena Regali Seleghim, Christoph Timm, Achim Grube, Matthias Köck, Gislene G. F. Nascimento, Ana Claudia T. Martins, Elissa G. O. Silva, Ana Olı́via de Souza, Paulo Roberto Regazi Minarini, Fábio C. S. Galetti, Célio Lopes Silva, Eduardo Hajdu, Roberto G. S. Berlinck,
Cyclostellettamines A – F (1 – 6) isolated from the sponge Pachychalina sp. and cyclostellettamines G - I, K and L (7 – 11) obtained by synthesis were evaluated in bioassays of antimicrobial activity against susceptible and antibiotic-resistant Staphylococcus aureus, Pseudomonas aeruginosa and antibiotic-susceptible Escherichia coli and Candida albicans, as well as in antimycobacterial activity against Mycobacterium tuberculosis H37Rv bioassays. The results obtained indicated that cyclostellettamines display ...
Tópico(s): Microbial Natural Products and Biosynthesis
2006 - Multidisciplinary Digital Publishing Institute | Marine Drugs
Produção Nacional
Revisado por pares
Lucimara Gaziola de la Torre, Rogério Silva Rosada, Ana Paula Fávaro Trombone, Fabiani Gai Frantz, Arlete Aparecida Martins Coelho‐Castelo, Célio Lopes Silva, Maria Helena Andrade Santana,
We present a comparative study of the physico-chemical properties, in vitro cytotoxicity and in vivo antibody production of surface-complexed DNA in EPC/DOTAP/DOPE (50/25/25% molar) liposomes and DOTAP/DOPE (50/50% molar) lipoplexes. The study aims to correlate the biological behavior and structural properties of the lipid carriers. We used DNA-hsp65, whose naked action as a gene vaccine against tuberculosis has already been demonstrated. Additionally, surface-complexed DNA-hsp65 in EPC/DOTAP/DOPE ( ...
Tópico(s): Lipid Membrane Structure and Behavior
2009 - Elsevier BV | Colloids and Surfaces B Biointerfaces
Produção Nacional
Revisado por pares
Denise Morais da Fonseca, Rogério Silva Rosada, Marina Oliveira e Paula, Pryscilla Fanini Wowk, Luis H. Franco, Edson Garcia Soares, Célio Lopes Silva, Vânia Luiza Deperon Bonato,
Tópico(s): vaccines and immunoinformatics approaches
2010 - Elsevier BV | Tuberculosis
Produção Nacional
Revisado por pares
Fabiani Gai Frantz, Rogério Silva Rosada, Camila Peres‐Buzalaf, Franciele R. T. Perusso, Vanderlei Rodrigues, Simone G. Ramos, Steven L. Kunkel, Célio Lopes Silva, Lúcia Helena Faccioli,
Background Helminthiasis and tuberculosis (TB) coincide geographically and there is much interest in exploring how concurrent worm infections might alter immune responses against bacilli and might necessitate altered therapeutic approaches. A DNA vaccine that codifies heat shock protein Hsp65 from M. leprae (DNAhsp65) has been used in therapy during experimental tuberculosis. This study focused on the impact of the co-existence of worms and TB on the therapeutic effects of DNAhsp65. Methodology/Principal ...
Tópico(s): Tuberculosis Research and Epidemiology
2010 - Public Library of Science | PLoS neglected tropical diseases
Produção Nacional
Revisado por pares
Fabiani Gai Frantz, Rogério Silva Rosada, Walter Miguel Turato, Camila Matias Peres, Arlete Aparecida Martins Coelho‐Castelo, Simone G. Ramos, David M. Aronoff, Célio Lopes Silva, Lúcia Helena Faccioli,
Mycobacterium tuberculosis and helminth infections coincide geographically and are classically described as TH1 and TH2 pathologies. There is much interest in exploring how concurrent worm infections might alter immune responses to mycobacterial infection. To explore this issue, mice were infected with Toxocara canis and co-infected with M. tuberculosis . Mice infected with M. tuberculosis had high numbers of neutrophils and mononuclear cells within the alveolar spaces, with increased parenchymal ...
Tópico(s): Parasitic Infections and Diagnostics
2007 - American Society of Tropical Medicine and Hygiene | American Journal of Tropical Medicine and Hygiene
Produção Nacional
Revisado por pares
Gabriel D. Victora, A Socorro-Silva, E C Volsi, K. Abdallah, Fernanda Lima, Robert B. Smith, Raquel Ajub Moysés, Carlos R. Zárate-Bladés, Pedro Michaluart, Célio Lopes Silva, Jorge Kalil, Verônica Coelho,
DNA-hsp65, a DNA vaccine encoding the 65-kDa heat-shock protein of Mycobacterium leprae (Hsp65) is capable of inducing the reduction of established tumors in mouse models. We conducted a phase I clinical trial of DNA-hsp65 in patients with advanced head and neck carcinoma. In this article, we report on the vaccine's potential to induce immune responses to Hsp65 and to its human homologue, Hsp60, in these patients. Twenty-one patients with unresectable squamous cell carcinoma of the head and neck ...
Tópico(s): Yersinia bacterium, plague, ectoparasites research
2009 - Springer Nature | Cancer Gene Therapy
Produção Nacional
Revisado por pares
M. H. Kossuga, Simone Possedente de Lira, Andréa Mendes do Nascimento, M.T.P. Gambardella, Roberto G. S. Berlinck, Yohandra Reyes Torres, Gislene G. F. Nascimento, Eli F. Pimenta, Márcio Luís Andrade e Silva, Otávio Henrique Thiemann, Glaucius Oliva, André G. Tempone, Márcia de Souza Carvalho Melhem, Ana Olı́via de Souza, Fábio C. S. Galetti, Célio Lopes Silva, Bruno C. Cavalcanti, Cláudia Pessoa, Manoel Odorico de Moraes, Eduardo Hajdu, Solange Peixinho, Rosana Moreira da Rocha,
ASCIDIAN Didemnum ligulum AND FROM THE OCTOCORAL Carijoa riisei.The investigation of extracts from six species of marine invertebrates yielded one new and several known natural products.Isoptilocaulin from the sponge Monanchora aff.arbuscula displayed antimicrobial activity at 1.3 μg/mL against an oxacillin-resistant strain of Staphylococcus aureus.Five inactive known dibromotyrosine derivatives, 2 -6, were isolated from a new species of marine sponge, Aplysina sp.The sponges Petromica ciocalyptoides ...
Tópico(s): Marine Sponges and Natural Products
2007 - Brazilian Chemical Society | Química Nova
Produção Nacional
Revisado por pares
Mirna Helena Regali Seleghim, Simone Possedente de Lira, M. H. Kossuga, Tatiana Batista, Roberto G. S. Berlinck, Eduardo Hajdu, Guilherme Muricy, Rosana Moreira da Rocha, Gislene G. F. Nascimento, Márcio Luís Andrade e Silva, Eli F. Pimenta, Otávio Henrique Thiemann, Glaucius Oliva, Bruno C. Cavalcanti, Cláudia Pessoa, Manoel Odorico de Moraes, Fábio C. S. Galetti, Célio Lopes Silva, Ana Olı́via de Souza, Solange Peixinho,
Herein we present the results of a screening with 349 crude extracts of Brazilian marine sponges, ascidians, bryozoans and octocorals, against 16 strains of susceptible and antibiotic-resistant bacteria, one yeast (Candida albicans), Mycobacterium tuberculosis H37Rv, three cancer cell lines MCF-7 (breast), B16 (murine melanoma ) and HCT8 (colon), and Leishmania tarentolae adenine phosphoribosyl transferase (L-APRT) enzyme. Less than 15% of marine sponge crude extracts displayed antibacterial activity, ...
Tópico(s): Synthesis and Biological Activity
2007 - Springer Science+Business Media | Revista Brasileira de Farmacognosia
Produção Nacional
Revisado por pares
Luciana Previato de Almeida, A. P. F. Trombone, Júlio CC Lorenzi, Carolina D. Rocha, Thiago Malardo, Isabela C Fontoura, Ana Flávia Gembre, Ricardo LL Silva, Célio Lopes Silva, Ademílson Panunto-Castelo, Arlete Aparecida Martins Coelho‐Castelo,
Although B cells are important as antigen presenting cells (APC) during the immune response, their role in DNA vaccination models is unknown.In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tuberculosis challenge.In vitro and in vivo studies showed that B cells efficiently present antigens after naked plasmid pcDNA3 encoding M. leprae 65-kDa heat shock protein (pcDNA3-Hsp65) internalization and protect B knock-out ( ...
Tópico(s): Hepatitis B Virus Studies
2011 - BioMed Central | Genetic Vaccines and Therapy
Produção Nacional
Revisado por pares
Carlos A. Sorgi, Elyara Maria Soares, Rogério Silva Rosada, Claudia S. Bitencourt, Karina Furlani Zoccal, Priscilla Aparecida Tártari Pereira, Caroline Fontanari, Izaíra Tincani Brandão, Ana Paula Masson, Simone G. Ramos, Célio Lopes Silva, Fabiani Gai Frantz, Lúcia Helena Faccioli,
The functions of eicosanoids, a family of potent biologically active lipid mediators, are not restricted to inflammatory responses and they also act as mediators of the pathogenesis process. However, the role of eicosanoids in tuberculosis remains controversial. To investigate the specific role of LTB4 in Mycobacterium tuberculosis (Mtb) infection, we used 5-lipoxygenase-deficient (5-LO-/-) mice and WT (sv129) mice inoculated intranasally with LTB4 (encapsulated in PLGA microspheres). We showed that ...
Tópico(s): Pneumonia and Respiratory Infections
2019 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
Produção Nacional
Revisado por pares
Rogério Silva Rosada, Lucimara Gaziola de la Torre, Fabiani Gai Frantz, Ana Paula Fávaro Trombone, Carlos R. Zárate-Bladés, Denise Morais da Fonseca, Patricia Souza, Izaíra Tincani Brandão, Ana Paula Masson, Edson García Soares, Simone G. Ramos, Lúcia Helena Faccioli, Célio Lopes Silva, Maria HA Santana, Arlete Aparecida Martins Coelho‐Castelo,
Abstract Background The greatest challenges in vaccine development include optimization of DNA vaccines for use in humans, creation of effective single-dose vaccines, development of delivery systems that do not involve live viruses, and the identification of effective new adjuvants. Herein, we describe a novel, simple technique for efficiently vaccinating mice against tuberculosis (TB). Our technique consists of a single-dose, genetic vaccine formulation of DNA-hsp65 complexed with cationic liposomes ...
Tópico(s): Antimicrobial Peptides and Activities
2008 - BioMed Central | BMC Immunology
Produção Nacional
Revisado por pares
Patricia Souza, Carlos R. Zárate-Bladés, Juliana I. Hori, Simone G. Ramos, Deison Soares de Lima, Tatiana Schneider, Rogério Silva Rosada, Lucimara Gaziola de la Torre, Maria Helena Andrade Santana, Izaíra Tincani Brandão, Ana Paula Masson, Arlete Aparecida Martins Coelho‐Castelo, Vânia Luiza Deperon Bonato, Fábio C. S. Galetti, Eduardo Dantas Casillo Gonçalves, Domingos Alexandre Ciccone Botte, Jeanne BM Machado, Célio Lopes Silva,
Background: Tuberculosis is a major threat to human health. The high disease burden remains unaffected and the appearance of extremely drug-resistant strains in different parts of the world argues in favor of the urgent need for a new effective vaccine. One of the promising candidates is heat-shock protein 65 when used as a genetic vaccine (DNAhsp65). Nonetheless, there are substantial data indicating that BCG, the only available anti-TB vaccine for clinical use, provides other important beneficial ...
Tópico(s): Escherichia coli research studies
2008 - Taylor & Francis | Expert Opinion on Biological Therapy
Produção Nacional
Revisado por pares
Pedro Michaluart, Kald Abdallah, Fernanda Lima, Rodney B.W. Smith, Raquel Ajub Moysés, Verônica Coelho, Gabriel D. Victora, A Socorro-Silva, E C Volsi, Carlos R. Zárate-Bladés, Alberto R. Ferraz, Alexandre Barreto, Maria Cristina Chammas, Renan Augusto Gomes, Eloísa Maria Mello Santiago Gebrim, Lica Arakawa‐Sugueno, Kristianne Porta Santos Fernandes, Paulo A. Lotufo, Maria Regina Alves Cardoso, Jorge Kalil, Célio Lopes Silva,
Considering that mycobacterial heat-shock protein 65 (hsp65) gene transfer can elicit a profound antitumoral effect, this study aimed to establish the safety, maximum-tolerated dose (MTD) and preliminary efficacy of DNA-hsp65 immunotherapy in patients with advanced head and neck squamous cell carcinoma (HNSCC). For this purpose, 21 patients with unresectable and recurrent HNSCC were studied. Each patient received three ultrasound-guided injections at 21-day intervals of: 150, 600 or 400 μg of DNA-hsp65. ...
Tópico(s): Bacteriophages and microbial interactions
2008 - Springer Nature | Cancer Gene Therapy
Produção Nacional
Revisado por pares
Célio Lopes Silva, Fernanda Calheta Vieira Portaro, Vânia Luiza Deperon Bonato, Antônio Carlos Martins de Camargo, Emer S. Ferro,
The initial processing of antigens leading to major histocompatibility complex (MHC) class I antigenic peptides is carried out by the proteasome. However, how the final epitopes are generated and protected from degradation by cytosolic peptidases remains unknown. Coincidentally, peptides associated with the MHC class I molecules range from 8 to 13 amino acid residues, similarly to the optimum substrate size required for the cytosolic thimet oligopeptidase. Here we have investigated the putative intracellular ...
Tópico(s): vaccines and immunoinformatics approaches
1999 - Elsevier BV | Biochemical and Biophysical Research Communications
Produção Nacional
Revisado por pares
Célio Lopes Silva, Vânia Luiza Deperon Bonato, Valéria Marçal Felix de Lima, Lúcia Helena Faccioli, Sylvia Cardoso Leão,
The memory/activated T cells, which mediate the long‐lived host response against tuberculosis, in mice immunized with either bacillus Calmette–Guérin (BCG) or mycobacterium heat‐shock protein 65 (hsp 65) antigen expressed from plasmid DNA (DNA‐hsp 65), were characterized. Protection against Mycobacterium tuberculosis challenge by DNA‐hsp 65 vaccination was associated with the presence of lymph node T‐cell populations in which CD8 + /CD44 hi interferon‐γ (IFN‐γ)‐producing/cytotoxic cells were prominent ...
Tópico(s): Immune Response and Inflammation
1999 - Wiley | Immunology