Produção Nacional
Revisado por pares
Nildimar Alves Honório, Rita Maria Ribeiro Nogueira, Cláudia Torres Codeço, Marília Sá Carvalho, Oswaldo Gonçalves Cruz, Mônica de Avelar Figueirêdo Mafra Magalhães, Josélio Maria Galvão de Araújo, Eliane Saraiva Machado de Araújo, Marcelo Quintela Gomes, Luciane Silva Pinheiro, Célio da Silva Pinel, Ricardo Lourenço‐de‐Oliveira,
Background Rio de Janeiro, Brazil, experienced a severe dengue fever epidemic in 2008. This was the worst epidemic ever, characterized by a sharp increase in case-fatality rate, mainly among younger individuals. A combination of factors, such as climate, mosquito abundance, buildup of the susceptible population, or viral evolution, could explain the severity of this epidemic. The main objective of this study is to model the spatial patterns of dengue seroprevalence in three neighborhoods with different ...
Tópico(s): COVID-19 epidemiological studies
2009 - Public Library of Science | PLoS neglected tropical diseases
Produção Nacional
Revisado por pares
Régis Resende Paulinelli, Ruffo Freitas‐Júnior, Clécio Ênio Murta de Lucena, Marise Amaral Rebouças Moreira, Vardeli Alves de Moraes, Júlio R. M. Bernardes-Junior, Célio da Silva Rocha Vidal, Alessandro Naldi Ruiz, Miliana Tostes Lucato, Nayara Gomes Silveira da Costa, Danilo Augusto Teixeira,
Abstract: To create an individualized predictive tool for the risk of malignancy in solid breast masses, based on echographic and clinical characteristics. Research Ethics Committee approval and informed consent were obtained. This multi-center study included 1,403 solid breast masses prospectively. Each ultrasound feature was analyzed and compared with the definitive diagnosis. The ultrasound results, women's ages and family histories of breast cancer were included in a multivariate logistic regression ...
Tópico(s): AI in cancer detection
2011 - Wiley | The Breast Journal
Produção Nacional
Revisado por pares
Régis Resende Paulinelli, Ruffo Freitas‐Júnior, Marise Amaral Rebouças Moreira, Vardeli Alves de Moraes, Júlio R. M. Bernardes-Junior, Célio da Silva Rocha Vidal, Alessandro Naldi Ruiz, Miliana Tostes Lucato,
Objective The purpose of this study was to assess the risk of malignancy for each type of sonographic feature in solid breast nodules. Methods The study included 304 patients from the Department of Gynecology and Obstetrics of the Federal University of Goiás who had solid breast nodules. A medical trainee, working under the supervision of a preceptor, obtained the sonographic images of the breast, and the features were recorded in a questionnaire. Each sonographic feature was analyzed and compared ...
Tópico(s): Cancer and Skin Lesions
2005 - Wiley | Journal of Ultrasound in Medicine
Produção Nacional
Revisado por pares
Daniel Cardoso Portela Câmara, Célio da Silva Pinel, Gláucio Pereira Rocha, Cláudia Torres Codeço, Nildimar Alves Honório,
In Brazil and several countries in the Americas, where dengue, chikungunya and Zika are cocirculating, there is a need to understand how different mosquito species relate to landscape and humans. Mosquito ecology and distribution, especially at finer spatial scales, are key factors to study since the relationship of mosquito communities to their habitats might have important consequences in the risk of disease transmission to humans. The aim of this study was to evaluate the diversity of resident ...
Tópico(s): Viral Infections and Vectors
2020 - Elsevier BV | Acta Tropica
Produção Nacional
Revisado por pares
Rodrigo A. da Silva, Célio Júnior da Costa Fernandes, Geórgia da Silva Feltran, Anderson Moreira Gomes, Amanda Fantini de Camargo Andrade, Denise Carleto Andia, Maikel P. Peppelenbosch, Willian Fernando Zambuzzi,
Abstract Whereas endothelial responses to shear stress are well‐characterized, the cell physiological effects of shear stress in smooth muscle cells (SMCs) remain largely obscure. As SMCs are directly challenged by shear stress after endothelial denuding injury following procedures such as angioplasty or endarterectomy, characterization of these responses represents an important scientific question. Hence we decided to contrast cytoskeletal reorganization, epigenetic reprogramming, signaling transduction, ...
Tópico(s): Signaling Pathways in Disease
2018 - Wiley | Journal of Cellular Physiology
Produção Nacional
Revisado por pares
Rodrigo Barros Rocha, A. R. Ramalho, Alexsandro Lara Teixeira, Bruno Galvêas Laviola, Francisco Célio Gomes da Silva, Júlio Sancho Linhares Teixeira Militão,
O objetivo deste trabalho foi estimar os parâmetros genéticos de características de qualidade de grãos de pinhão-manso (Jatropha curcas) e quantificar o progresso genético da seleção. O teor de óleo dos grãos e os pesos de grãos, amêndoas e casca de 120 plantas, selecionadas ao acaso em plantio comercial, foram avaliados aos 36 e 48 meses de cultivo. O progresso genético foi quantificado por meio da avaliação dos ganhos diretos e da utilização de índices de seleção. O efeito da interação genótipos ...
Tópico(s): Agricultural and Food Sciences
2012 - Embrapa Informação Tecnológica | Pesquisa Agropecuária Brasileira
Produção Nacional
Revisado por pares
Erielma Borges de Queiroz, Franciele Cossetim da Silva, Célio Borella, Márcio da Silva Araújo, Edson Hirose, Flávio Gonçalves de Jesus,
The importance of Spodoptera cosmioides (Lepidoptera: Noctuidae) in soybean crops has been growing, mainly with the cultivation of transgenic Bt plants. The objective of this study was to evaluate antixenosis to S. cosmioides in soybean plants with different leaf colors and trichome densities. Antioxenosis was evaluated by non-preference for feeding and oviposition assays. The study was performed in no-choice and free-choice tests using the genotypes Anta 82, BMX Desafio, BMX Potência, BRS 397, BRS ...
Tópico(s): Legume Nitrogen Fixing Symbiosis
2020 - Springer Science+Business Media | Phytoparasitica
Produção Nacional
Revisado por pares
Iracilda Zeppone Carlos, Diana Bridon da Graça Sgarbi, Jayme Angluster, Celuta S. Alviano, Célio Lopes Silva,
Tópico(s): Natural product bioactivities and synthesis
1992 - Springer Science+Business Media | Mycopathologia
Produção Nacional
Revisado por pares
Ariane Marumoto, Renato Milani, Rodrigo A. da Silva, Célio Júnior da Costa Fernandes, José Mauro Granjeiro, Carmen V. Ferreira, Maikel P. Peppelenbosch, Willian Fernando Zambuzzi,
The reciprocal and adaptive interactions between cells and substrates governing morphological transitions in the osteoblast compartment remain largely obscure. Here we show that osteoblast cultured in basement membrane matrix (Matrigel™) exhibits significant morphological changes after ten days of culture, and we decided to exploit this situation to investigate the molecular mechanisms responsible for guiding osteoblast morphological transitions. As almost all aspects of cellular physiology are under ...
Tópico(s): MicroRNA in disease regulation
2017 - Elsevier BV | Bone
Produção Nacional
Augusto Santana Nascimento, Luisa L. Peres, Alessandra V.S. Fari, Renato Milani, Rodrigo A. da Silva, Célio Júnior da Costa Fernandes, Maikel P. Peppelenbosch, Carmen Veríssima Ferreira‐Halder, Willian Fernando Zambuzzi,
Breast cancer is responsible for 25% of cancer cases and 15% of cancer death among women. Treatment is usually prolonged and hampered by the development of chemoresistance. The molecular mechanisms maintaining the chemoresistant phenotype remains, however, largely obscure. As kinase signaling in general is highly drugable, identification of kinases essential for maintaining chemoresistance could prove therapeutically useful. Hence we compared cellular kinase activity in chemotherapy resistant MCF7Res ...
Tópico(s): Bioinformatics and Genomic Networks
2017 - Impact Journals LLC | Oncotarget
Produção Nacional
Revisado por pares
Débora Zanoni do Prado, Samara Louzada Oliveira, Clarissa Hamaio Okino‐Delgado, Susann Auer, Jutta Ludwig‐Müller, Magali Ribeiro da Silva, Célio Júnior da Costa Fernandes, Caio Antônio Carbonari, William Fernando Zambuzzi, Luciana Francisco Fleuri,
In recent years, forest breeding programs have increased Eucalyptus production for commercial purposes; however, high-performing Eucalyptus clones have had problems with propagation, especially when rooting. Nevertheless, studies have shown that inoculation of microorganisms producing indole-3-acetic acid (IAA) is an especially productive procedure to help these clones breed. In this study, we evaluated the production of IAA and analogues in 16 microbial strains. For the first time, a high IAA production ...
Tópico(s): Mycorrhizal Fungi and Plant Interactions
2019 - Elsevier BV | Journal of Cleaner Production
Produção Nacional
Revisado por pares
Iracilda Zeppone Carlos, Monica Magalh�es Costa Zini, Diana Bridon da Gra�a Sgarbi, J Angluster, Celuta S. Alviano, Célio Lopes Silva,
Tópico(s): Yeasts and Rust Fungi Studies
1994 - Springer Science+Business Media | Mycopathologia
Produção Nacional
Revisado por pares
Daniel Cardoso Portela Câmara, Cláudia Torres Codeço, Tania Ayllón, Aline Araújo Nobre, Renata Campos Azevedo, Davis Ferreira, Célio da Silva Pinel, Gláucio Pereira Rocha, Nildimar Alves Honório,
Using collection methods for Aedes adults as surveillance tools provides reliable indices and arbovirus detection possibilities. This study compared the effectiveness of different methods for collecting Ae. aegypti and Ae. albopictus and detecting arboviruses circulating in field-caught female specimens. Collection sites were defined in urban, peri-urban, and rural landscapes in two Brazilian cities. Collections were performed using Adultraps (ADT), BG-Sentinel (BGS), CDC-like traps (CDC), and indoor ( ...
Tópico(s): Viral Infections and Vectors
2022 - Multidisciplinary Digital Publishing Institute | Tropical Medicine and Infectious Disease
Produção Nacional
Revisado por pares
Eduardo D. C. Gonçalves, Vânia Luiza Deperon Bonato, Denise Morais da Fonseca, Edson García Soares, Izaíra Tincani Brandão, Ana Paula M Soares, Célio Lopes Silva,
Vaccines are considered by many to be one of the most successful medical interventions against infectious diseases. But many significant obstacles remain, such as optimizing DNA vaccines for use in humans or large animals. The amount of doses, route and easiness of administration are also important points to consider in the design of new DNA vaccines. Heterologous prime-boost regimens probably represent the best hope for an improved DNA vaccine strategy. In this study, we have shown that heterologous ...
Tópico(s): Immune Response and Inflammation
2007 - BioMed Central | Genetic Vaccines and Therapy
Produção Nacional
Revisado por pares
Lúcia Maria Carareto Alves, Flor�ncio Figueiredo, Sebasti�o L�zaro Brand�o Filho, I. Tincani, Célio Lopes Silva,
Tópico(s): Women's cancer prevention and management
1987 - Springer Science+Business Media | Mycopathologia
Produção Nacional
Revisado por pares
Rubens R. Santos, Alexandrina Sartori, Deison Soares de Lima, Patricia Souza, Arlete Aparecida Martins Coelho‐Castelo, Vânia Luiza Deperon Bonato, Célio Lopes Silva,
Our group previously demonstrated that a DNA plasmid encoding the mycobacterial 65-kDa heat shock protein (DNA-HSP65) displayed prophylactic and therapeutic effect in a mice model for tuberculosis. This protection was attributed to induction of a strong cellular immunity against HSP65. As specific immunity to HSP60 family has been detected in arthritis, multiple sclerosis and diabetes, the vaccination procedure with DNA-HSP65 could induce a cross-reactive immune response that could trigger or worsen ...
Tópico(s): Immunotherapy and Immune Responses
2009 - Springer Science+Business Media | Journal of Immune Based Therapies and Vaccines
Produção Nacional
Revisado por pares
K M Lima, S. A. Santos, R. R. Santos, Izaíra Tincani Brandão, J. M. Rodrigues, Célio Lopes Silva,
A DNA vaccine codifying the mycobacterial hsp65 can prevent infection with Mycobacterium tuberculosis in a prophylactic setting and also therapeutically reduce the number of bacteria in infected mice. The protective mechanism is thought to be related to Th1-mediated events that result in bacterial killing. To determine the best method of hsp65 introduction for vaccination efficacy against tuberculosis (TB), we evaluated the immunogenicity and protection of DNA–hsp65 administered by gene gun bombardment ...
Tópico(s): RNA and protein synthesis mechanisms
2003 - Elsevier BV | Vaccine
Produção Nacional
Revisado por pares
Douglas B. Lowrie, Ricardo E. Tascon, M. Joseph Colston, Célio Lopes Silva,
Expression of the gene for a single mycobacterial antigen (Mycobacterium leprae hsp65) in adult Balb/c mice resulted in substantial cell-mediated protection against challenge with M. tuberculosis. CD4 and CD8 T cells cloned from spleens of such immunized mice passively transferred protection to non-immunized mice, and CD8 cells selectively lysed macrophages infected with M. tuberculosis. Three modes of expressing the gene have been tested: (1) expression from a retroviral vector (pZIPNeoSV) in implanted ...
Tópico(s): Mycobacterium research and diagnosis
1994 - Elsevier BV | Vaccine
Produção Nacional
Revisado por pares
Pedro Michaluart, Kald Abdallah, Fernanda Lima, Rodney B.W. Smith, Raquel Ajub Moysés, Verônica Coelho, Gabriel D. Victora, A Socorro-Silva, E C Volsi, Carlos R. Zárate-Bladés, Alberto R. Ferraz, Alexandre Barreto, Maria Cristina Chammas, Renan Augusto Gomes, Eloísa Maria Mello Santiago Gebrim, Lica Arakawa‐Sugueno, Kristianne Porta Santos Fernandes, Paulo A. Lotufo, Maria Regina Alves Cardoso, Jorge Kalil, Célio Lopes Silva,
Considering that mycobacterial heat-shock protein 65 (hsp65) gene transfer can elicit a profound antitumoral effect, this study aimed to establish the safety, maximum-tolerated dose (MTD) and preliminary efficacy of DNA-hsp65 immunotherapy in patients with advanced head and neck squamous cell carcinoma (HNSCC). For this purpose, 21 patients with unresectable and recurrent HNSCC were studied. Each patient received three ultrasound-guided injections at 21-day intervals of: 150, 600 or 400 μg of DNA-hsp65. ...
Tópico(s): Bacteriophages and microbial interactions
2008 - Springer Nature | Cancer Gene Therapy
Produção Nacional
Revisado por pares
F. Figueiredo, Lúcia Maria Carareto Alves, Célio Lopes Silva,
Tumour necrosis factor (TNF) was detected in serum from mice challenged with Paracoccidioides brasiliensis. The serum TNF levels of mice challenged with an avirulent strain were significantly higher than those of mice challenged with a virulent strain, and the same was observed for the TNF levels of mice challenged with a cell wall fraction (F1) from the two fungal strains. Fraction F1 consisted of chitin and beta-glucan; but although the chitin contents were similar for the two strains, the avirulent ...
Tópico(s): Antifungal resistance and susceptibility
1993 - Oxford University Press | Clinical & Experimental Immunology
Produção Nacional
Revisado por pares
R.R. Santos-Junior, Alexandrina Sartori, Marcelo De Franco, Orlando Garcia Ribeiro Filho, Arlete Aparecida Martins Coelho‐Castelo, Vânia Luiza Deperon Bonato, Wafa Hanna Koury Cabrera, Olga M. Ibañez, Célio Lopes Silva,
We described a prophylactic and therapeutic effect of a DNA vaccine encoding the Mycobacterium leprae 65-kDa heat shock protein (DNA-hsp65) in experimental murine tuberculosis. However, high homology of the vaccine to the corresponding mammalian hsp60, together with the CpG motifs in the plasmidial vector, could trigger or exacerbate an autoimmune disease. In the present study, we evaluate the potential of DNA-hsp65 vaccination to induce or modulate arthritis in mice genetically selected for acute ...
Tópico(s): Immune Response and Inflammation
2005 - Mary Ann Liebert, Inc. | Human Gene Therapy
Produção Nacional
Revisado por pares
Karla M. Lima, S. A. Santos, J. M. Rodrigues, Célio Lopes Silva,
The use of protein or DNA in vaccination process rather than living or attenuated microorganism, aims at the increase of the vaccines safety. However, in these cases, the use of adjuvant is frequently required to improve their immunogenicity. In this study, we show the importance of the adjuvant in a vaccine formulation. Vaccines for tuberculosis provide an instructive example, based on the mycobacterial 65 kDa heat shock protein (hsp65). The same antigen can elicit completely different patterns ...
Tópico(s): Escherichia coli research studies
2004 - Elsevier BV | Vaccine
Produção Nacional
Revisado por pares
Vânia Luiza Deperon Bonato, E. D. C. Gonçalves, Edson García Soares, R. R. Santos Júnior, Alexandrina Sartori, Arlete Aparecida Martins Coelho‐Castelo, Célio Lopes Silva,
A DNA vaccine based on the heat-shock protein 65 Mycobacterium leprae gene (pHSP65) presented a prophylactic and therapeutic effect in an experimental model of tuberculosis. In this paper, we addressed the question of which protective mechanisms are activated in Mycobacterium tuberculosis-infected mice after immune therapy with pHSP65. We evaluated activation of the cellular immune response in the lungs of infected mice 30 days after infection (initiation of immune therapy) and in those of uninfected ...
Tópico(s): Immune Response and Inflammation
2004 - Wiley | Immunology
Produção Nacional
Revisado por pares
Douglas B. Lowrie, Célio Lopes Silva,
Our studies in mice show that DNA vaccines, initially designed to prevent infection, can have a dramatic therapeutic action too. In heavily infected mice, simply by giving DNA vaccination, the immune response can be caused to switch from one that is relatively inefficient and gives bacterial stasis to one that kills the bacteria, and persistent bacteria can be eliminated. Adoptive transfer of protection with T cell clones and in vitro tests of clone function indicate that the effects are probably ...
Tópico(s): Pneumocystis jirovecii pneumonia detection and treatment
2000 - Elsevier BV | Vaccine
Produção Nacional
Revisado por pares
Douglas B. Lowrie, Ricardo E. Tascon, Celio L. Silva,
Recent findings in mice have changed our perception of how protective immunity works in tuberculosis and hold promise for the rapid development of new vaccines. For example, we now know: (1) that a single mycobacterial protein antigen can be sufficient to generate powerful protective immunity, provided that it is presented to the immune system in the right way; (2) that the expression of protection depends on cytotoxic antigen-specific T cells; (3) that the identity of the antigen may be less important ...
Tópico(s): Immunotherapy and Immune Responses
1995 - Karger Publishers | International Archives of Allergy and Immunology
Produção Nacional
Revisado por pares
Valéria Marçal Felix de Lima, Vânia Luiza Deperon Bonato, Karla M. Lima, S. A. Santos, Rubens R. Santos, Eduardo D. C. Gonçalves, Lúcia Helena Faccioli, Izaíra Tincani Brandão, José M. Rodrigues-Junior, Célio Lopes Silva,
ABSTRACT Mice treated with viable Mycobacterium tuberculosis with no glycolipid trehalose dimycolate (TDM) on the outer cell wall (delipidated M. tuberculosis ) by intraperitoneal or intratracheal inoculation presented an intense recruitment of polymorphonuclear cells into the peritoneal cavity and an acute inflammatory reaction in the lungs, respectively. In addition, lung lesions were resolved around the 32nd day after intratracheal inoculation. TDM-loaded biodegradable poly- dl -lactide-coglycolide ...
Tópico(s): Inhalation and Respiratory Drug Delivery
2001 - American Society for Microbiology | Infection and Immunity
Produção Nacional
Revisado por pares
Karla M. Lima, Vânia Luiza Deperon Bonato, Lúcia Helena Faccioli, Izaíra Tincani Brandão, S. A. Santos, Arlete Aparecida Martins Coelho‐Castelo, Sylvia Cardoso Leão, Célio Lopes Silva,
The way to deliver antigens and cellular requirements for long-lasting protection against tuberculosis are not known. Immunizations with mycobacterial 65 kDa heat shock protein (hsp65) expressed from J774-hsp65 cells (antigen-presenting cells that endogenously produce hsp65 antigen) or from plasmid DNA, or with the protein entrapped in cationic liposomes, can each give protective immunity similar to that obtained from live Bacillus Calmette Guérin (BCG), whereas injecting the protein in Freund's incomplete ...
Tópico(s): vaccines and immunoinformatics approaches
2001 - Elsevier BV | Vaccine
Produção Nacional
Revisado por pares
Over the last few years, some of our experiments in which mycobacterial heat-shock protein (HSP) antigens were presented to the immune system as if they were viral antigens have had a significant impact on our understanding of protective immunity against tuberculosis. They have also markedly enhanced the prospects for new vaccines. We now know that the mycobacterial HSP65 antigen can confer protection equal to that from live BCG vaccine in mice.
Tópico(s): Escherichia coli research studies
1999 - Elsevier BV | Microbes and Infection
Produção Nacional
Revisado por pares
Douglas B. Lowrie, Ricardo E. Tascon, Vânia Luiza Deperon Bonato, Valéria Marçal Felix de Lima, Lúcia Helena Faccioli, Evangelos Stavropoulos, M. Joseph Colston, R. Glyn Hewinson, Karin Moelling, Célio Lopes Silva,
Tópico(s): Immune responses and vaccinations
1999 - Nature Portfolio | Nature
Produção Nacional
Revisado por pares
Carlos R. Zárate-Bladés, Vânia Luiza Deperon Bonato, Eduardo L. V. Silveira, Marina Oliveira e Paula, Cristina M. Junta, Paula Sandrin‐Garcia, Ana Lúcia Fachin, Stephano S. Mello, Renato S. Cardoso, Fabio Cícero de Sá Galetti, Arlete Aparecida Martins Coelho‐Castelo, Simone G. Ramos, Eduardo Antônio Donadi, Elza Tiemi Sakamoto‐Hojo, Geraldo Aleixo Silva Passos, Célio Lopes Silva,
The continued increase in tuberculosis (TB) rates and the appearance of extremely resistant Mycobacterium tuberculosis strains (XDR-TB) worldwide are some of the great problems of public health. In this context, DNA immunotherapy has been proposed as an effective alternative that could circumvent the limitations of conventional drugs. Nonetheless, the molecular events underlying these therapeutic effects are poorly understood.We characterized the transcriptional signature of lungs from mice infected ...
Tópico(s): interferon and immune responses
2008 - Wiley | The Journal of Gene Medicine