Raed Sulaiman, Pradip De, Jennifer C. Aske, Xiaoqian Lin, Adam Dale, Ethan Vaselaar, Cheryl Ageton, Kris Gaster, Luis Rojas Espaillat, David Starks, Nandini Dey,
... such as circulating cancer-associated macrophage-like cells (CAMLs). We report for the first time the identification and in-depth morphologic characterization of CAMLs in patients with endometrial cancers. We isolated CAMLs by size-based filtration on lithographically fabricated membranes ... pathological parameters, 98% of patients were positive for CAMLs. Two size-based subtypes of CAMLs, 20 µm (giant) CAMLs, of distinctive polymorphic morphologies with mononuclear or fused ... in several morphological states were observed, including apoptotic CAMLs, CAML−WBC doublets, conjoined CAMLs, CAML−WBC clusters, ...
Tópico(s): Neuroblastoma Research and Treatments
2022 - Multidisciplinary Digital Publishing Institute | Cancers
Kirby P. Gardner, Massimo Cristofanilli, Saranya Chumsri, Rena Lapidus, Cha‐Mei Tang, Ashvathi Raghavakaimal, Daniel L. Adams,
... into the circulation. Cancer-associated macrophage-like cells (CAMLs) are pro-tumor polynucleated monocytic cells of hematopoietic ... between Beta-2 adrenergic receptor (B2AR) signaling in CAMLs and CTCs by establishing expression profiles in a ... MDA-MB-231). We compared the model to CAMLs and CTCs found in patents. Although internalization events ... B2AR between the tumor cell lines and the CAMLs found in patients. High B2AR expression on patients’ CAMLs was correlated with significantly more CAMLs in the ...
Tópico(s): Neuropeptides and Animal Physiology
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
... basic protein/calcium signal‐modulating cyclophilin ligand (WRB/CAML) and Get1p/Get2p complexes, in vertebrates and yeast, ... While WRB appears to exist in all eukaryotes, CAML homologs were previously recognized only among chordates, raising the question as to how CAML's function is performed in other phyla. Furthermore, ... was recognized as the metazoan homolog of Get1, CAML and Get2, although functionally equivalent, were not considered to be homologous. CAML contains an N‐terminal basic, TRC40/Get3‐interacting, ...
Tópico(s): Galectins and Cancer Biology
2020 - Wiley | Traffic
Sha Sun, Malaiyalam Mariappan,
... membrane protein insertion complex composed of WRB and CAML proteins (22Vilardi F. Lorenz H. Dobberstein B. WRB ... Clancy A. Janshoff A. Schwappach B. WRB and CAML are necessary and sufficient to mediate tail-anchored ... a crucial role in fixing the topology of CAML (25Inglis A.J. Page K.R. Guna A. ... modes of orphan subunit recognition for the WRB/CAML complex.Cell Rep. 2020; 30 (32187542): 3691-3698. ... required for the correct integration of its partner CAML into the ER.Sci. Rep. 2019; 9 (31417168): ... 6) Google Scholar). In the absence of WRB, CAML exhibits two different topologies: one with both TMD2 ...
Tópico(s): RNA and protein synthesis mechanisms
2020 - Elsevier BV | Journal of Biological Chemistry
Francisco Fernández‐Avilés, Ricardo Sanz-Ruíz, Andreu M. Climent, Lina Badimón, Roberto Bolli, Dominique Charron, Valentı́n Fuster, Stefan Janssens, Jens Kastrup, Hyo-Soo Kim, Thomas F. Lüscher, John F. Martin, Philippe Menasché, Fausto J. Pinto, Robert D. Simari, Gregg W. Stone, André Terzic, James T. Willerson, Joseph C. Wu,
... Serviço de Cardiologia, Hospital Universitário de Santa Maria, CAML, Centro Cardiovascular da Universidade de Lisboa, Universidade de ... Serviço de Cardiologia, Hospital Universitário de Santa Maria, CAML, Centro Cardiovascular da Universidade de Lisboa, Universidade de ... Serviço de Cardiologia, Hospital Universitário de Santa Maria, CAML, Centro Cardiovascular da Universidade de Lisboa, Universidade de ... Serviço de Cardiologia, Hospital Universitário de Santa Maria, CAML, Centro Cardiovascular da Universidade de Lisboa, Universidade de ...
Tópico(s): Congenital heart defects research
2018 - Lippincott Williams & Wilkins | Circulation Research
Jennifer C. Shing, Lonn D. Lindquist, Nica Borgese, Richard J. Bram,
Calcium-modulating cyclophilin ligand (CAML) is an endoplasmic reticulum (ER) protein that functions, along with WRB and TRC40, to mediate tail-anchored (TA) protein insertion into the ER membrane. Physiologic roles for CAML include endocytic trafficking, intracellular calcium signaling, and the ... protein insertion. To identify a possible role for CAML in cancer cells, we generated Eμ-Myc transgenic ... that carry a tamoxifen-inducible deletion allele of Caml. In multiple B-cell lymphoma cell lines derived ...
Tópico(s): Pancreatic function and diabetes
2017 - Springer Nature | Cell Death Discovery
Daniel L. Adams, Diane K. Adams, Steven H. Lin, Massimo Cristofanilli, Raymond C. Bergan, Jeffrey R. Marks, Stuart S. Martin, Saranya Chumsri, Thai H. Ho, Rena G. Lapidus, Susan Tsai, Cha‐Mei Tang, R. Katherine Alpaugh,
11503 Background: Cancer Associated Macrophage-Like cells (CAMLs) are a recently described circulating stromal cell subtype commonly found in the peripheral blood of patients in all stages of solid malignancies and ... study was undertaken to evaluate the relationship of CAMLs and overall survival (OS) in 6 solid tumor ... blood was filtered by CellSieve microfiltration assay and CAMLs enumerated, as previously described. Patients were grouped by CAML number ( < 6 or ≥6) and by size ( < 49 ... ratios (HR) by censored univariate & multivariate analysis. Results: CAMLs were identified in 93% of samples, averaging 8. ...
Tópico(s): Pancreatic and Hepatic Oncology Research
2017 - Lippincott Williams & Wilkins | Journal of Clinical Oncology
Sara Colombo, Silvia Cardani, Annalisa Maroli, Adriana Vitiello, Paolo Soffientini, Arianna Crespi, Richard F. Bram, Roberta Benfante, Nica Borgese,
... protein), homologous to yeast Get1, in combination with CAML (calcium-modulating cyclophilin ligand), which is not homologous ... we investigated the role of endogenous WRB and CAML in tail-anchored protein insertion as well as ... extract depleted of TRC40-associated proteins or of CAML itself, whereas in vitro synthesized CAML and WRB together were sufficient to confer insertion competence to liposomes. CAML was found to be in ∼5-fold excess ... of the other one; in the case of CAML silencing, this effect was attributable to destabilization of ...
Tópico(s): Toxin Mechanisms and Immunotoxins
2016 - Elsevier BV | Journal of Biological Chemistry
Jianling Li, Rongjie Huang, Weiyong Liao, Zhaoni Chen, Shijun Zhang, Renbin Huang,
... library. We identified calcium modulating cyclophilin-binding ligand (CAML) as a novel interacting partner of DENV C protein. We report for the first time that CAML influenced DENV production. DENV production was significantly attenuated in CAML knock-down cells at 36 h post-infection. CAML did not influence DENV entry, genome uncoating, viral ... translation and virus secretion. Our study pinpointed that CAML influenced the process of apoptosis by altering mitochondrial ... from 36 h post-infection. Over-expression of CAML protected Huh7 cells from apoptosis and knock down ...
Tópico(s): Viral Infections and Outbreaks Research
2012 - Elsevier BV | Biochemical and Biophysical Research Communications
Mohammed Ali, J. Hammonds, Lingmei Ding, Paul Spearman,
... defined. We recently identified calcium-modulating cyclophilin ligand (CAML) as a Vpu-interacting protein that also restricts particle release. We hypothesized that CAML may act to enhance tetherin-mediated restriction of ... and responsiveness to Vpu, while levels of cellular CAML protein did not. Tetherin but not CAML was inducible by interferon in a wide variety of human cells. Stable depletion of human CAML in restrictive HeLa cells had no effect on ... cells permissive and Vpu-unresponsive. Tetherin but not CAML expression in permissive human cells rendered them restrictive ...
Tópico(s): Mosquito-borne diseases and control
2010 - Public Library of Science | PLoS ONE
Yu Liu, Liviu Malureanu, Karthik B. Jeganathan, David Tran, Lonn D. Lindquist, Jan M. van Deursen, Richard J. Bram,
AbstractCalcium modulating cyclophilin ligand (CAML) is a ubiquitously expressed cytoplasmic protein that is implicated in the EGFR and LCK signaling pathways and required for early ... To further define the critical biological functions of CAML at the cellular level, we generated CAML-deleted mouse embryonic fibroblasts (MEFs) using an in ... loxP mediated conditional knockout system. We found that CAML–/– MEFs have severely impaired proliferation and a strong ... of normal anaphases. The primary mitotic defect of CAML–/– MEFs is that duplicated chromosomes fail to segregate ...
Tópico(s): Signaling Pathways in Disease
2009 - Taylor & Francis | Cell Cycle
Emmanuel Chailloux, Christian Foisy,
Caml-Flight is a data-parallel extension for Caml Light. It is an intermediate, portable and mostly ... memory multiprocessors (DMM). Its current implementation modifies the Caml Light compiler to introduce new instructions for the ... define a kind of persistence to communicate values. Caml Light has now evolved to become Objective Caml, including an object extension and a native compiler. ... distribution, we propose a new implementation called Objective Caml Flight. To do so we use new libraries and tools included in Objective Caml such as multi-threading, persistence and parsers, i. ...
Tópico(s): Distributed systems and fault tolerance
2003 - World Scientific | Parallel Processing Letters
Irena Marjanović, Jelena Kostic, Bojana Stanić, Nadja Pejanovic, Bojana Lucic, Teodora Karan-Djurašević, Dragana Janić, Lidija Dokmanović, Srdja Janković, Nada Suvajdžić, Dragica Tomin, Ognjen Perišić, Goran Rakočević, Miloš A. Popović, Sonja Pavlović, Nataša Tos̆ić,
... data. We analyzed DNA samples from 20 childhood (cAML) and 20 adult AML (aAML) patients, using NGS ... 2981 × per amplicon. A total of 412 (207 cAML, 205 aAML) variants in the coding regions were detected; out of which, only 122 (62 cAML and 60 aAML) were potentially protein-changing. Our ... frequent single gene AML associated mutations differed in cAML and aAML patient cohorts: IDH1 (0 % cAML, 5 % aAML), IDH2 (0 % cAML, 10 % aAML), NPM1 (10 % cAML, 35 % aAML). Additionally, potentially protein-changing variants were ... associated proteins (JAK3, ABL1, GNAQ, and EGFR) in cAML, while among aAML, the prevalence is directed towards ...
Tópico(s): Epigenetics and DNA Methylation
2016 - SAGE Publishing | Tumor Biology
Christine Nitschke, Benedikt Markmann, Leonie Konczalla, Jolanthe Kropidlowski, Thais Pereira‐Veiga, Pasquale Scognamiglio, Martin Schönrock, Marianne Sinn, Marie Tölle, Jakob R. Izbicki, Klaus Pantel, Faik G. Uzunoğlu, Harriet Wikman,
Background: Circulating Cancer Associated Macrophage-like cells (CAMLs) have been described as novel liquid biopsy analytes and unfavorable prognostic markers in some tumor entities, with scarce data for Pancreatic Ductal Adenocarcinomas ( ... based cell enrichment approach (ParsortixTM) was used for CAML detection, followed by immunofluorescence staining using pan-keratin, CD14, and CD45 antibodies to differentiate between CAMLs, circulating tumor cells (CTCs), and leukocytes. Results: CAMLs were detectable at baseline in 36.1% of ...
Tópico(s): Renal and related cancers
2022 - Multidisciplinary Digital Publishing Institute | Biomedicines
Hugo J. F. Carvalho, Andrea Del Bondio, Francesca Maltecca, Sara Colombo, Nica Borgese,
Abstract Calcium-modulating cyclophilin ligand (CAML), together with Tryptophan rich basic protein (WRB, Get1 in yeast), constitutes the mammalian receptor for the Transmembrane Recognition Complex subunit of ... TA) proteins to the endoplasmic reticulum (ER) membrane. CAML has also been implicated in other cell-specific ... cell types. Notwithstanding the stoichiometric imbalance, WRB and CAML depend strictly on each other for expression. Here, we investigated the mechanism by which WRB impacts CAML levels. We demonstrate that CAML, generated in the ...
Tópico(s): Adenosine and Purinergic Signaling
2019 - Nature Portfolio | Scientific Reports
Fabio Vilardi, Milena Stephan, Anne Clancy, Andreas Janshoff, Blanche Schwappach,
... and the recently identified membrane receptors WRB and CAML. Here, we used yeast lacking the GET1 and GET2 genes and substituted them with WRB and CAML. This rescued the growth phenotypes of the GET receptor mutant. We demonstrate that WRB and CAML efficiently recruit Get3 to the ER membrane and ... results show that the membrane spanning segments of CAML are essential to create a functional receptor with ... the binding parameters of TRC40 to the WRB/CAML receptor. We conclude that together, WRB and CAML ...
Tópico(s): Fungal and yeast genetics research
2014 - Public Library of Science | PLoS ONE
Nicholas A. Zane, Justin H. Gundelach, Lonn D. Lindquist, Richard J. Bram,
Calcium-modulating cyclophilin ligand (CAML) is a ubiquitously expressed protein that is important during thymopoiesis. However, whether it serves a function in mature lymphocytes is unknown. In this article, we show that CAML is essential for survival of peripheral follicular (Fo) B cells. Conditional deletion of CAML in CD19-Cre transgenic mice caused a significant ... cell numbers and increased rates of homeostatic proliferation. CAML-deficient Fo cells showed increased cellular turnover and normal proliferative ability. Although CAML-deficient Fo cells responded to AgR stimulation and ...
Tópico(s): T-cell and B-cell Immunology
2012 - American Association of Immunologists | The Journal of Immunology
David Tran, Contessa E. Edgar, Karin L. Heckman, Shari L. Sutor, Catherine J. Huntoon, Jan van Deursen, David L. McKean, Richard J. Bram,
Calcium modulating cyclophilin ligand (CAML) is a ubiquitously expressed protein implicated in T cell signaling, although its mechanism and physiologic role in the immune system are unknown. We show here that CAML is essential for peripheral T cell development. Inactivation of CAML in mouse thymocytes lowered the numbers of double- ... positive and enhanced negative selection. We found that CAML interacts with p56Lck and appears to regulate subcellular ... resting and T cell receptor (TCR)-stimulated cells. CAML-deficient cells displayed enhanced p56lck and ZAP-70 ...
Tópico(s): Galectins and Cancer Biology
2005 - Cell Press | Immunity
Shaodong Guo, Marco López-Ilasaca, Victor J. Dzau,
... ATRAP as bait identified calcium-modulating cyclophilin ligand (CAML) as an ATRAP partner. Yeast two-hybrid and ... demonstrated that the N-terminal hydrophilic domain of CAML (amino acids (aa) 1-189) mediates a specific interaction between ATRAP and CAML. Our analysis also showed that aa 40-82 ... activity. Overexpression of ATRAP decreased Ang II- or CAML-induced NFAT transcriptional activation, whereas an ATRAP-interacting domain of CAML (aa 1-189) sensitized NFAT activation in response to Ang II. These results indicate that CAML is an important signal transducer for the actions ...
Tópico(s): Heat shock proteins research
2005 - Elsevier BV | Journal of Biological Chemistry
Stephen C. Tovey, Martin D. Bootman, Peter Lipp, Michael J. Berridge, Richard J. Bram,
The Ca2+-modulating cyclophilin ligand (CAML) protein causes stimulation of transcription factors via activation of a store-operated Ca2+ entry pathway. Since CAML is widely expressed in mammalian tissues, it may ... the present study, we investigated the consequence of CAML overexpression on Ca2+ signaling using rapid confocal imaging of Fluo3-loaded NIH3T3 fibroblasts. Control and CAML-expressing cells gave concentration-dependent responses to the Ca2+ mobilizing agonist ATP. CAML expression reduced the sensitivity of the cells so ...
Tópico(s): Neuroscience and Neural Engineering
2000 - Elsevier BV | Biochemical and Biophysical Research Communications
Michael P. Holloway, Richard J. Bram,
Ca-modulating cyclophilin ligand (CAML) was originally described as a cyclophilin B-binding protein whose overexpression in T cells causes a rise in intracellular calcium, ... As reported here, structure-function analysis of the CAML gene in Jurkat T cells indicates that two of CAML's putative membrane-spanning domains are necessary and ... propose that the hydrophobic C-terminal tail of CAML forms its effector domain, thus implicating the N- ... in intracellular calcium signaling. Ca-modulating cyclophilin ligand (CAML) was originally described as a cyclophilin B-binding ...
Tópico(s): Signaling Pathways in Disease
1996 - Elsevier BV | Journal of Biological Chemistry
Bradley R. Moore, Steven J. Parker, Matthew H. Pinkerton,
... to differentiate between the morphologically similar grenadiers Macrourus caml and M. whitsoni and validate species identifications by ... Otolith shape information was collected from 610 M. caml and 329 M. whitsoni otoliths from research collections, ... between the two species, with otoliths of M. caml being larger and more elongate than those of ... location on otolith shape was evident for M. caml. Otolith shape of M. whitsoni varied slightly among ... was 96.2%, with 96.8% of M. caml and 94.8% of M. whitsoni correctly classified. ...
Tópico(s): Identification and Quantification in Food
2022 - Elsevier BV | Fisheries Research
Daniel J. Gironda, Daniel L. Adams, Jianzhong He, Ting Xu, Hui Gao, Yawei Qiao, Ritsuko Komaki, James M. Reuben, Zhongxing Liao, Mariela Blum‐Murphy, Wayne L. Hofstetter, Cha‐Mei Tang, Steven H. Lin,
Abstract Background Cancer Associated Macrophage-Like cells (CAMLs) are polynucleated circulating stromal cells found in the bloodstream of numerous solid-tumor malignancies. Variations within CAML size have been associated with poorer progression free ... III EC patients (n = 32) receiving chemoradiotherapy (CRT). CAML sizes were sequentially monitored prior to CRT (BL), ~ ... the completion of CRT (T2). Results We found CAMLs in 88% (n = 28/32) of all patient ... PFS and OS was found in patients with CAMLs < 50 μm by the completion of CRT over ...
Tópico(s): Esophageal and GI Pathology
2020 - BioMed Central | Journal of Translational Medicine
Siaw-Li Chan, Lonn D. Lindquist, Michael J. Hansen, Megan A. Girtman, Larry R. Pease, Richard J. Bram,
Abstract Calcium-modulating cyclophilin ligand (CAML) is an endoplasmic reticulum resident protein that is widely expressed. Although it has been demonstrated to participate in the tail-anchored ... we show that mature, peripheral T cells require CAML for survival specifically following TCR-induced activation. In ... from spleen and lymph nodes of tamoxifen-inducible CAML knockout mice (tCAML−/−). Whereas CAML-deficient T cells were able to express the ... proliferated less and died. Cells did not require CAML for entry into the S phase of the ...
Tópico(s): Galectins and Cancer Biology
2015 - American Association of Immunologists | The Journal of Immunology
Yasunori Yamamoto, Toshiaki Sakisaka,
... membrane insertion. We identify calcium-modulating cyclophilin ligand (CAML) as a mammal-specific receptor for TRC40, an ... targeting newly synthesized TA proteins, and show that CAML mediates membrane insertion of TA proteins. We show that CAML binds to WRB, an evolutionarily conserved TRC40 receptor, through the transmembrane domains and that CAML and WRB synergistically insert TA proteins into the membrane. Mutagenesis of CAML demonstrates that binding of TRC40 to CAML is required to ensure synergistic membrane insertion. Thus, ...
Tópico(s): Galectins and Cancer Biology
2012 - Elsevier BV | Molecular Cell
David Tran, H. R. Russell, Shari L. Sutor, Jan van Deursen, Richard J. Bram,
Calcium-modulating cyclophilin ligand (CAML) is a ubiquitous protein that has been implicated in signaling from the cell surface receptor TACI in lymphocytes, although its ... its function in the mouse, we disrupted the CAML gene and found it to be required for early embryonic development, but not for cellular viability. CAML-deficient cells have severely impaired proliferative responses to ... receptor (EGFR) are normal in the absence of CAML, the recycling of internalized receptors to the plasma ... to its reduced surface accumulation. We demonstrate that CAML normally associates directly with the kinase domain of ...
Tópico(s): Metabolism, Diabetes, and Cancer
2003 - Elsevier BV | Developmental Cell
Michael P. Holloway, Richard J. Bram,
The calcium-modulating cyclophilin ligand (CAML) protein activates Ca2+ influx signaling when overexpressed in Jurkat T cells. Although CAML appears to directly participate in Ca2+-dependent signaling initiated by the transmembrane activator and CAML interactor cell surface receptor, its mechanism of action ... discontinuous sucrose gradients and indirect immunofluorescence indicate that CAML co-localizes with sarcoplasmic/endoplasmic reticulum calcium/ATPase- ... indicate that the hydrophilic NH2-terminal domain of CAML is directed toward the cytoplasm. Functionally, CAML overexpression ...
Tópico(s): Force Microscopy Techniques and Applications
1998 - Elsevier BV | Journal of Biological Chemistry
Thirze Hermans, Andrew J. Dougill, Stephen Whitfield, Caroline L. Peacock, Samuel Eze, Christian Thierfelder,
... 39% lower for CP) and infiltration (Lemu CAM/CAML 0.15 cms−1, CP 0.09 cms−1; Mwansambo CP/CAM 0.14 cms−1, CAML 0.18 cms−1). In the conventional practice, ... 0 mgkg −1, CAM 49.4 mgkg −1, CAML 51.7 mgkg −1), nitrate/nitrite values (Mwansambo ... 7 mgkg −1, CAM 171.9 mgkg −1, CAML 103.3 mgkg −1). This perception contributes to ... measurements under CA (Mwansambo CP 3225 kgha-1, CAML 5067 kgha-1, CAM 5160 kgha-1; Lemu CP 2886 kgha-1, CAM 2872 kgha-1, CAML 3454 kgha-1 ). The perceived carbon benefits of ...
Tópico(s): Sustainable Agricultural Systems Analysis
2021 - Elsevier BV | Journal of Environmental Management
Daniel L. Adams, Diane K. Adams, R. Katherine Alpaugh, Massimo Cristofanilli, Stuart S. Martin, Saranya Chumsri, Cha-Mei Tang, Jeffrey R. Marks,
... clinical use. Circulating cancer-associated macrophage-like cells (CAML) are specialized myeloid cells found in peripheral blood ... with the presence of solid malignancies. We measured CAMLs prospectively in peripheral blood to ascertain their prevalence, ... studies: 1) CellSieve microfilters were used to isolate CAMLs from blood samples of patients with known malignant ... core-needle biopsy to diagnose suspicious breast masses.CAMLs were found in 93% of known malignant patients ( ... In subjects undergoing core biopsy for initial diagnosis, CAMLs were found in 88% of subjects with invasive ...
Tópico(s): Cancer Genomics and Diagnostics
2016 - American Association for Cancer Research | Cancer Epidemiology Biomarkers & Prevention