María Eugenia Soria, Carlos García‐Crespo, Brenda Martínez‐González, Lucía Vázquez-Sirvent, Rebeca Lobo-Vega, Ana Isabel de Ávila, Isabel Gallego, Qian Chen, Damir García‐Cehic, Meritxell Llorens-Revull, Carlos Briones, Jordi Gómez, Cristina Ferrer‐Orta, Núria Verdaguer, Josep Gregori, Francisco Rodríguez‐Frías, Marı́a Buti, Juan Ignacio Esteban, Esteban Domingo, Josep Quer, Celia Perales,
Despite the high virological response rates achieved with current directly acting antiviral agents (DAAs) against hepatitis C virus (HCV), around 2% to 5% of treated patients do not achieve a sustained viral response. The identification of amino acid substitutions associated with treatment failure requires analytical designs, such as subtype-specific ultradeep sequencing (UDS) methods, for HCV characterization and patient management. Using this procedure, we have identified six highly represented amino ...
Tópico(s): HIV/AIDS drug development and treatment
2020 - American Society for Microbiology | Journal of Clinical Microbiology
Brenda Martínez‐González, María Eugenia Soria, Lucía Vázquez-Sirvent, Cristina Ferrer‐Orta, Rebeca Lobo-Vega, Pablo Mínguez, Lorena de la Fuente, Carlos Lloréns, Beatriz Soriano, Ricardo Ramos, Marta Cortón, Rosario López‐Rodríguez, Carlos García‐Crespo, Isabel Gallego, Ana Isabel de Ávila, Jordi Gómez, Luis Enjuanes, Llanos Salar‐Vidal, Jaime Esteban, R. Fernández‐Roblas, Ignacio Gadea, Carmen Ayuso, Javier Ruiz‐Hornillos, Núria Verdaguer, Esteban Domingo, Celia Perales,
Mutant spectra of RNA viruses are important to understand viral pathogenesis and response to selective pressures. There is a need to characterize the complexity of mutant spectra in coronaviruses sampled from infected patients. In particular, the possible relationship between SARS-CoV-2 mutant spectrum complexity and disease associations has not been established. In the present study, we report an ultradeep sequencing (UDS) analysis of the mutant spectrum of amplicons from the nsp12 (polymerase)- ...
Tópico(s): Bacteriophages and microbial interactions
2022 - American Society for Microbiology | Microbiology Spectrum
Brenda Martínez‐González, María Eugenia Soria, Lucía Vázquez-Sirvent, Cristina Ferrer‐Orta, Rebeca Lobo-Vega, Pablo Mínguez, Lorena de la Fuente, Carlos Lloréns, Beatriz Soriano, Ricardo Ramos, Marta Cortón, Rosario López‐Rodríguez, Carlos García‐Crespo, Pilar Somovilla, Antoni Durán‐Pastor, Isabel Gallego, Ana Isabel de Ávila, Soledad Delgado, Federico Morán, Cecilio López-Galı́ndez, Jordi Gómez, Luis Enjuanes, Llanos Salar‐Vidal, Mario Esteban‐Muñoz, Jaime Esteban, R. Fernández‐Roblas, Ignacio Gadea, Carmen Ayuso, Javier Ruiz‐Hornillos, Núria Verdaguer, Esteban Domingo, Celia Perales,
Populations of RNA viruses are composed of complex and dynamic mixtures of variant genomes that are termed mutant spectra or mutant clouds. This applies also to SARS-CoV-2, and mutations that are detected at low frequency in an infected individual can be dominant (represented in the consensus sequence) in subsequent variants of interest or variants of concern. Here we briefly review the main conclusions of our work on mutant spectrum characterization of hepatitis C virus (HCV) and SARS-CoV-2 at the ...
Tópico(s): Plant Virus Research Studies
2022 - Multidisciplinary Digital Publishing Institute | Pathogens