Natalya Seredkina, Svetlana N. Zykova, Ole Petter Rekvig,
... lupus nephritis coincided with an accumulation of activated caspase 3-positive cells in kidneys, in addition to ... lupus nephritis coincided with an accumulation of activated caspase 3-positive cells in kidneys, in addition to ... factor receptor superfamily, member 1a) toward activation of caspases through involvement of adaptor proteins Fas (TNFRSF6)-associated ... minutes with primary antibody to stain for activated caspase 3 (rabbit anti-mouse active caspase 3 antibody diluted 1:500, R&D Systems, Abinodon, UK), activated caspase 9 (rabbit anti-mouse activated caspase 9 antibody ...
Tópico(s): interferon and immune responses
2009 - Elsevier BV | American Journal Of Pathology
Jerry Shen, Ying Yin, Jietang Mai, Xinyu Xiong, Meghana Pansuria, Jingshan Liu, Erin Maley, Najam Us Saqib, Hong Wang, Xiaofeng Yang,
... sites have been considered to be similar between caspase-1 and caspase-9, which do not support their differential functions ... acid residues in the cleavage sites in 34 caspase-1 and 11 capase-9 experimentally identified substrates. ... made the following findings: first, we verified that caspase-1 and caspase-9 shared 100% aspartic acid in the P1 ... the structures in the cleavage sites of most caspase-1 substrates are different from that of caspase-9 substrates in the following three aspects, (a) ... the 91.4% cleavage sites of 35 new caspase-1 substrates. Conclusion Since most caspase-1 substrates ...
Tópico(s): interferon and immune responses
2009 - Elsevier BV | Atherosclerosis
Stephanie C. Eisenbarth, Richard A. Flavell,
... Cias1) forms an intracellular multimolecular complex with active caspase-1, called an inflammasome, creating a platform for ... In contrast, NLR-induced forms of apoptosis–pyroptosis (caspase-1 and ASC dimerization-dependent) and pyronecrosis (ASC-dependent but caspase-1-independent)–are inflammatory and share features with ... inhibitory repeat (BIR)-like domain/NLRB family; (3) caspase-recruitment domain (CARD)/NLRC family; and (4) pyrin ... containing a CARD) and the active form of caspase-1 (Martinon et al, 2002). Through the proteolytic ...
Tópico(s): Immune Cell Function and Interaction
2009 - Springer Nature | EMBO Molecular Medicine
Shizue Ohsawa, Shun Hamada, Hiroaki Asou, Keiuke Kuida, Yasuo Uchiyama, Hiroki Yoshida, Masayuki Miura,
Caspases are essential in multicellular organisms for inducing cell death during normal development and in the immune system. However, caspases can also trigger the degenerative process under certain ... Semaphorin 7A (Sema7A) as a novel substrate for caspase-9 that can be used to monitor caspase-9 activity in mice, and found nonapoptotic caspase-9 activation in the aged olfactory bulb (OB). Immunostaining of the OB for the caspase-9-cleaved form of Sema7A revealed abundant caspase-9-activated cells in 2-year-old (aged) ...
Tópico(s): Neurobiology and Insect Physiology Research
2009 - Society for Neuroscience | Journal of Neuroscience
Seungkirl Ahn, Ji‐Hee Kim, Makoto R. Hara, Xiu-Rong Ren, Robert J. Lefkowitz,
... for human BAD), Bcl-xL (1:1,000), caspase-3 (1:1,000), cleaved caspase-3 (Asp175) (1:1,000), phospho-ERK1/2 ( ... Text PDF PubMed Scopus (407) Google Scholar).Cleaved Caspase-3 and DNA Fragmentation Assays-VSMCs were serum- ... overnight, cell lysates were prepared. Pro- and cleaved caspase-3 were visualized, and the amounts of cleaved caspase-3 were quantified as described for immunoblotting in ... Text PDF PubMed Scopus (407) Google Scholar). For caspase-3 immunoblotting, VSMC lysates were prepared using the ... effects of AngII stimulation on the activation of caspase-3 (cleavage of procaspase-3) induced by apoptotic ...
Tópico(s): Adipose Tissue and Metabolism
2009 - Elsevier BV | Journal of Biological Chemistry
Wei Pan, Li S. da Graca, Yufang Shao, Qian Yin, Hao Wu, Xuejun Jiang,
... as an apoptotic enhancer that stimulates apoptosome-mediated caspase activation. In this study, we defined the structural requirement for its activity to stimulate caspase activation using a series of truncation mutants of ... yet an oncoprotein, is not able to stimulate caspase activation. A highly discrete region between these two proteins localizes to an essential caspase activation motif of PHAPI. Additionally, PHAPI is predominantly ... as an apoptotic enhancer that stimulates apoptosome-mediated caspase activation. In this study, we defined the structural ...
Tópico(s): Ubiquitin and proteasome pathways
2009 - Elsevier BV | Journal of Biological Chemistry
Xiaofang Wang, Ge Lin, Kristen Martins‐Taylor, Hui Zeng, Ren‐He Xu,
... observed here can be markedly prevented by the caspase inhibitor z-VAD-FMK. We further demonstrated that ... 2-interacting mediator of cell death and the caspase-ROCK1-myosin signaling. Anoikis is independent of pre- ... cells: preventing them from anoikis via inhibition of caspase activation. Apoptosis and proliferation are two dynamically and ... observed here can be markedly prevented by the caspase inhibitor z-VAD-FMK. We further demonstrated that ... 2-interacting mediator of cell death and the caspase-ROCK1-myosin signaling. Anoikis is independent of pre- ...
Tópico(s): CRISPR and Genetic Engineering
2009 - Elsevier BV | Journal of Biological Chemistry
Małgorzata Sztiller-Sikorska, Justyna Jakubowska, Michał Woźniak, Marta Stasiak, Małgorzata Czyż,
... was undertaken to explore the potential contribution of caspase-3 to pharmacologically-induced differentiation of K562 cells. ... of doxorubicin, hydroxyurea, cytosine arabinoside, cisplatin and haemin. Caspase-3 activation, mitochondrial membrane potential dissipation and viability ... with low concentrations of the tested drugs activated caspase-3 but did not trigger detectable apoptosis. Instead, elevated levels of haemoglobin-positive and glycophorin A/caspase-3-double-positive cells were observed, suggesting involvement ...
Tópico(s): PARP inhibition in cancer therapy
2009 - Wiley | British Journal of Pharmacology
H. Seda Vatansever, Kadriye Sorkun, S. İsmet Gürhan, F. Özdal-Kurt, Elgin Türköz, Ömür Gençay, Bekir Salih,
... apoptotic effect of propolis extracts (PE) on the caspase pathway in the human breast cell line MCF- ... method (terminal deoxynucleotidyltransferase-biotin nick end-labelling) and caspase activity was investigated by immunocytochemistry using antibodies directed against caspase 6, caspase 8 and caspase 9. The results showed that the PE 5 ... exposed to the other extracts. Immunpositivity for all caspases was detected after treatment with all the extracts and at all dilutions, with stronger immunoreactivity for caspase 6 than caspases 8 and 9. Caspase 6 ...
Tópico(s): Insect and Pesticide Research
2009 - Elsevier BV | Acta Histochemica
Dominique Talabot‐Ayer, Céline Lamacchia, Cem Gabay, Gaby Palmer,
... IL-33 was reported to be cleaved by caspase-1 to generate an 18-kDa fragment, which ... IL-33 receptor T1/ST2. However, the proposed caspase-1 cleavage site is poorly conserved between species. In addition, it is not clear whether caspase-1 cleavage of pro-IL-33 occurs in ... and bioactivity. In this study, we further investigated caspase-1 cleavage of mouse and human pro-IL- ... cell lysates, which was enhanced by incubation with caspase-1. However, in vitro assays of mouse and ... not a direct substrate for this enzyme. Consistently, caspase-1 activation in THP-1 cells induced cleavage ...
Tópico(s): Eosinophilic Esophagitis
2009 - Elsevier BV | Journal of Biological Chemistry
Xiaoyan Wu, Rongqing Guo, Peili Chen, Quan Wang, Patrick N. Cunningham,
... located in the endothelium. We showed previously that caspase inhibition protected mice against LPS-induced AKI and ... LPS-induced renal inflammation. Therefore we hypothesized that caspase activation amplifies TNF-induced inflammation in renal endothelial ... cultured renal ECs, TNF induced apoptosis through a caspase-8-dependent pathway. TNF caused translocation of the ... 1 and VCAM-1. However, the broad-spectrum caspase inhibitor Boc-d-fmk reduced NF-kB activation ... real-time PCR and flow cytometry. Broad-spectrum caspase inhibition markedly inhibited neutrophil adherence to the TNF- ...
Tópico(s): Inflammasome and immune disorders
2009 - American Physical Society | AJP Renal Physiology
Shihoko Okazaki, Fumihide Ogawa, Yohei Iwata, Toshihide Hara, Eiji Muroi, Kazuhiro Komura, Motoi Takenaka, Kazuhiro Shimizu, Minoru Hasegawa, Manabu Fujimoto, Shinichi Sato,
... the presence or levels of antibodies (Abs) against caspase-3 and their clinical relevance in systemic sclerosis (SSc). Anti-caspase-3 Ab was examined by enzyme-linked immunosorbent assay and immunoblotting. IgG anti-caspase-3 Ab levels in SSc patients were higher ... normal controls. SSc patients positive for IgG anti-caspase-3 Ab had significantly longer disease duration, more ... serum immunoglobulin and erythrocyte sedimentation rates. IgG anti-caspase-3 Ab levels correlated positively with serum IgG ... isoprostane. Immunoblotting analysis confirmed the presence of anti-caspase-3 Ab in sera from SSc patients. Caspase- ...
Tópico(s): Eosinophilic Disorders and Syndromes
2009 - Springer Science+Business Media | Rheumatology International
Dieter Demon, Petra Van Damme, Tom Vanden Berghe, Annelies Deceuninck, Joost Van Durme, Jelle Verspurten, Kenny Helsens, Francis Impens, Magdalena Wejda, Joost Schymkowitz, Frédéric Rousseau, Annemieke Madder, Joël Vandekerckhove, Wim Declercq, Kris Gevaert, Peter Vandenabeele,
Caspase-3 and -7 are considered functionally redundant proteases with similar proteolytic specificities. We performed a proteome- ... diagonal chromatography technology and identified 46 shared, three caspase-3-specific, and six caspase-7-specific cleavage sites. Further analysis of these ... the P5 position contributes to the discrimination between caspase-7 and -3 specificity. One of the caspase-7-specific substrates, the 40 S ribosomal protein ... derived P6–P5′ undecapeptide retained complete specificity for caspase-7. The corresponding P6–P1 hexapeptide still displayed ...
Tópico(s): Machine Learning in Bioinformatics
2009 - Elsevier BV | Molecular & Cellular Proteomics
Mohamed Lamkanfi, Thirumala‐Devi Kanneganti,
Caspase-7 was considered to be redundant with caspase-3 because these related cysteine proteases share an ... endogenous protein substrates in common. In addition, both caspases are proteolytically activated by the initiator caspase-8 and -9 during death receptor- and DNA- ...
Tópico(s): interferon and immune responses
2009 - Elsevier BV | The International Journal of Biochemistry & Cell Biology
Mohamed Lamkanfi, Lilian O. Moreira, Patrudu Makena, Diana C.J. Spierings, Kelli L. Boyd, Peter J. Murray, Douglas R. Green, Thirumala‐Devi Kanneganti,
... to the excessive mortality associated with these disorders. Caspase inhibitors confer protection from endotoxin-induced lymphocyte apoptosis ... improve survival, but it is not clear which caspases mediate lipopolysaccharide (LPS)-induced lymphocyte apoptosis and mortality. We report here that the apoptotic executioner caspase-7 was activated in the splenocytes of LPS-injected mice, suggesting a role for caspase-7 in lymphocyte apoptosis. Indeed, caspase-7-deficient mice were resistant to LPS-induced ...
Tópico(s): Cell death mechanisms and regulation
2009 - Elsevier BV | Blood
Jeejabai Radhakrishnan, Iyad M. Ayoub, Raúl J. Gazmuri,
... accompanied by the release of cytochrome c and caspase-3 activation. We now investigated the role of caspase-3 activation by examining whether such process prompts apoptotic DNA fragmentation, whether caspase-3 inhibition attenuates myocardial dysfunction, and whether myocardial ... hydrogen exchanger isoform-1 (NHE-1) inhibition involve caspase-3 inhibition using a rat model of ventricular ... cytosolic cytochrome c, decreases in pro- and cleaved caspase-9 fragments, increases in 17-kDa caspase-3 fragments, and increases in caspase-3 activity ...
Tópico(s): Cardiac Ischemia and Reperfusion
2009 - American Physical Society | AJP Heart and Circulatory Physiology
Yayoi Kamata, Aya Taniguchi, Mami Yamamoto, Junko Nomura, Kazuhíko Ishihara, Hidenari Takahara, Toshihiko Hibino, Atsushi Takeda,
... BH from those peptides. On the other hand, caspase 14 degraded deiminated filaggrin into a few peptides ... BH from those peptides. On the other hand, caspase 14 degraded deiminated filaggrin into a few peptides ... channel-activating serine protease 1/Prss 8, and caspase 14 in mice (26List K. Szabo R. Wertz ... of the deiminated filaggrin by calpain I or caspase 14. Based on these results, we proposed that ... I was purchased from Calbiochem (Darmstadt, Germany). Human caspase 14 was from BIOMOL International LP (Plymouth, PA). ...
Tópico(s): Allergic Rhinitis and Sensitization
2009 - Elsevier BV | Journal of Biological Chemistry
Katrin Breitbach, Guang Sun, Jens Köhler, Kristin Eske, Patimaporn Wongprompitak, Gladys Tan, Yi‐Chun Liu, Yunn‐Hwen Gan, Ivo Steinmetz,
... has been shown that B. pseudomallei can induce caspase-1-dependent cell death in macrophages. The aim ... study was to further elucidate the role of caspase-1 during B. pseudomallei infection. In vivo experiments with caspase-1 −/− mice revealed a high susceptibility to B. ... 24 h after infection compared to control animals. caspase-1 −/− bone marrow-derived macrophages (BMM) exhibited strong caspase-3 expression and reduced cell damage compared to ... apoptosis, whereas WT BMM showed signs of rapid caspase-1-dependent cell death. Moreover, we found that ...
Tópico(s): Burkholderia infections and melioidosis
2009 - American Society for Microbiology | Infection and Immunity
Irmgard Paris, Carolina Perez-Pastene, Eduardo Couve, Pablo Caviedes, Susan P. LeDoux, Juan Segura‐Aguilar,
... externalization with 5 μm cyclosporine A; lack of caspase-3 activation; formation of autophagic vacuoles containing mitochondria ... copper·dopamine complex induces mitochondrial autophagy followed by caspase-3-independent apoptotic cell death. However, a different ... externalization with 5 μm cyclosporine A; lack of caspase-3 activation; formation of autophagic vacuoles containing mitochondria ... copper·dopamine complex induces mitochondrial autophagy followed by caspase-3-independent apoptotic cell death. However, a different ...
Tópico(s): Alzheimer's disease research and treatments
2009 - Elsevier BV | Journal of Biological Chemistry
Lawrence JK Wee, Joo Chuan Tong, Tin Wee Tan, Shoba Ranganathan,
Abstract Background Caspases belong to a class of cysteine proteases which function as critical effectors in cellular processes such as apoptosis ... expected to be discovered, the elucidation of the caspase degradome will be an important milestone in the ... health and disease. Several computational methods for predicting caspase cleavage sites have been developed recently for identifying ... cleavage site prediction and these factors to predict caspase substrates. Sequences are first predicted for cleavage sites ...
Tópico(s): Machine Learning in Bioinformatics
2009 - BioMed Central | BMC Genomics
Zhong Jiang, Nicole M. Fletcher, Michael P. Diamond, Husam M. Abu‐Soud, Ghassan M. Saed,
... observed in adhesion fibroblasts is caused by lower caspase‐3 activity due to an increase in caspase‐3 S ‐nitrosylation. For this study, we have ... peroxynitrite and cell lysates were immunoprecipitated with anti‐caspase‐3 polyclonal antibody. The biotinylated proteins were detected using a nitrosylation detection kit. Caspase‐3 activity and apoptosis were measured by colorimetric and TUNEL assays, respectively. Our results showed that caspase‐3 S ‐nitrosylation is significantly higher in adhesion ... S ‐nitrosylation resulted in a 30% decrease in caspase‐3 activity in adhesion fibroblasts. Peroxynitrite treatment resulted ...
Tópico(s): Vitamin C and Antioxidants Research
2009 - Wiley | Wound Repair and Regeneration
Yoshinori Nishimoto, Daisuke Ito, Takuya Yagi, Yoshihiro Nihei, Yoshiko Tsunoda, Norihiro Suzuki,
... formation using TDP-43-expressing cells. Under apoptosis, caspase-3 generates two 35-kDa (p35f) and 25-kDa (p25f) fragments. However, in caspase-3(−/−) cells, novel caspase-3-independent isoforms of these two variants (p35iso ... formation using TDP-43-expressing cells. Under apoptosis, caspase-3 generates two 35-kDa (p35f) and 25-kDa (p25f) fragments. However, in caspase-3(−/−) cells, novel caspase-3-independent isoforms of these two variants (p35iso ... al. focused on proteolytic cleavage and demonstrated that caspase-3 can mediate cleavage of TDP-43 to ...
Tópico(s): Genetic Neurodegenerative Diseases
2009 - Elsevier BV | Journal of Biological Chemistry
Young Eun Choi, Michael Butterworth, Srinivas Malladi, Colin S. Duckett, Gerald M. Cohen, Shawn B. Bratton,
... apoptosis largely through direct inhibition of the initiator caspase-9 and the effector caspase-3 and -7. Two additional IAP family members, ... and cIAP2, were once thought to also inhibit caspases, but more recent studies have suggested otherwise. Here ... not significantly inhibit the proteolytic activities of effector caspases on fluorogenic or endogenous substrates. However, cIAP1 does bind to caspase-3 and -7 and does so, remarkably, at ... terminus of the large subunit of fully mature caspase-7, whereas cIAP1 bound to partially processed caspase- ...
Tópico(s): Cancer-related Molecular Pathways
2009 - Elsevier BV | Journal of Biological Chemistry
Marcello D’Amelio, Virve Cavallucci, Francesco Cecconi,
Caspases are a family of cysteinyl aspartate-specific proteases that are highly conserved in multicellular organisms and ... regulators of apoptosis. A member of this family, caspase-3, has been identified as a key mediator ... studies in snail, fly and rat suggest that caspase-3 also functions as a regulatory molecule in ... to providing an overview of the mechanism of caspase-3 activation, we review genetic and pharmacological studies of apoptotic and nonapoptotic functions of caspase-3 and discuss the regulatory mechanism of caspase- ...
Tópico(s): Autophagy in Disease and Therapy
2009 - Springer Nature | Cell Death and Differentiation
Teruyoshi Uetani, Hironao Nakayama, Hideki Okayama, Takafumi Okura, Jitsuo Higaki, Hirofumi Inoue, Shigeki Higashiyama,
... under hypoxic conditions. Furthermore, we observed an increased Caspase-3 activity, reactive oxygen species accumulation, and an ... the uc-proHB-EGF transfectants with inhibitors of Caspase-3, reactive oxygen species, and JNK, namely, Z- ... under hypoxic stress leads to cardiomyocyte apoptosis via Caspase-3- and JNK-dependent pathways. Heparin-binding epidermal ... under hypoxic conditions. Furthermore, we observed an increased Caspase-3 activity, reactive oxygen species accumulation, and an ... the uc-proHB-EGF transfectants with inhibitors of Caspase-3, reactive oxygen species, and JNK, namely, Z- ...
Tópico(s): Ubiquitin and proteasome pathways
2009 - Elsevier BV | Journal of Biological Chemistry
Saverio Tardito, Claudio Isella, Enzo Médico, Luciano Marchiò, Elena Bevilacqua, Maria Hatzoglou, Ovidio Bussolati, R. Franchi‐Gazzola,
... extensive endoplasmic reticulum vacuolization in the absence of caspase activation. A wide panel of human cancer cell ... extensive endoplasmic reticulum vacuolization in the absence of caspase activation. A wide panel of human cancer cell ... HT1080 human fibrosarcoma cells, the latter induces typical caspase-dependent apoptosis, while A0 inhibits caspase-3 activity and elicits a death process lacking ... A possible explanation is that A0, by inhibiting caspase-3 (18.Tardito S. Bussolati O. Gaccioli F. ... UV-visible spectra indicated the ligand-metal complexation. Caspase inhibitors (Sample Pack from R&D Systems) were ...
Tópico(s): RNA regulation and disease
2009 - Elsevier BV | Journal of Biological Chemistry
Bilal Amarneh, Krista A. Matthews, Robert B. Rawson,
... flies lacking dS2P are viable. The Drosophila effector caspase Drice cleaves dSREBP, and cleavage requires an Asp ... 386, in the cytoplasmic juxtamembrane stalk. The initiator caspase Dronc does not cleave dSREBP, but animals lacking ... They do not require Dcp1, although this effector caspase also can cleave dSREBP in vitro. Cleavage of ... flies lacking dS2P are viable. The Drosophila effector caspase Drice cleaves dSREBP, and cleavage requires an Asp ... 386, in the cytoplasmic juxtamembrane stalk. The initiator caspase Dronc does not cleave dSREBP, but animals lacking ...
Tópico(s): Invertebrate Immune Response Mechanisms
2009 - Elsevier BV | Journal of Biological Chemistry
Noelia Fradejas‐Villar, María Dolores Pastor, Miguel Burgos, Rudi Beyaert, Pedro Tranque, Soledad Calvo,
... aim in this work was to determine whether caspase‐11, able to activate apoptotic and proinflammatory pathways, ... death in ischemic conditions. According to our results, caspase‐11 is up‐regulated in primary astrocyte cultures ... thapsigargin and tunicamycin. Moreover, these same stimuli increased caspase‐11 mRNA levels and luciferase activity driven by a caspase‐11 promoter, indicating that caspase‐11 is regulated at the transcriptional level. Our ... the involvement of ER stress‐associated CHOP in caspase‐11 regulation, insofar as CHOP overexpression by means ...
Tópico(s): RNA regulation and disease
2009 - Wiley | Journal of Neuroscience Research
Sang Choel Lee, Seung Hyeok Han, Jin Ji Li, Sun Ha Lee, Dong-Sub Jung, Seung-Jae Kwak, Seung Hye Kim, Dong Ki Kim, Tae‐Hyun Yoo, Jin Hyun Kim, Se-Ho Chang, Dae Suk Han, Shin‐Wook Kang,
... ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated ... ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated ... 05) (Figure 4). Bax and active fragments of caspase-3 protein expression were significantly increased, whereas Bcl- ... 2 (P<0.01) and active fragments of caspase-3 protein expression in DM glomeruli (P<0. ... to Bax, Bcl-2, and active fragments of caspase-3 protein expression, apoptosis in glomeruli was assessed ...
Tópico(s): Dialysis and Renal Disease Management
2009 - Elsevier BV | Kidney International
Darren Finlay, Amy L. Howes, Kristiina Vuori,
Caspase-8 has a well-defined canonical role as an apical protease of the extrinsic apoptosis pathway. ... other nonapoptotic functions. We have previously shown that caspase-8 is required for efficient adhesion-induced activation ... Erk)-1/2 pathway. We now show that caspase-8 is also necessary for the efficient activation ... activation is independent of the proteolytic activity of caspase-8 and can be recapitulated using only the ... In addition, we identify specific residues within the caspase-8 "RXDLL motif" that are essential for Erk ...
Tópico(s): NF-κB Signaling Pathways
2009 - American Association for Cancer Research | Cancer Research