Catarina Milheiriço, Hermı́nia de Lencastre, Alexander Tomasz,
Most methicillin-resistant Staphylococcus aureus (MRSA) strains are resistant to beta-lactam antibiotics due to the presence of the mecA gene, encoding an extra penicillin-binding protein (PBP2A) that has low affinity for virtually all beta-lactam antibiotics. Recently, a new resistance determinant-the mecC gene-was identified in S. aureus isolates recovered from humans and dairy cattle. Although having typically low MICs to beta-lactam antibiotics, MRSA strains with the mecC determinant are also ...
Tópico(s): Biochemical and Structural Characterization
2017 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Choon Kim, Catarina Milheiriço, Hermı́nia de Lencastre, Alexander Tomasz,
Studies with methicillin-resistant Staphylococcus aureus (MRSA) strain COL have shown that the optimal resistance phenotype requires not only mecA but also a large number of "auxiliary genes" identified by Tn551 mutagenesis. The majority of auxiliary mutants showed greatly increased levels of oxacillin resistance when grown in the presence of sub-MICs of mupirocin, suggesting that the mechanism of reduced resistance in the auxiliary mutants involved the interruption of a stringent stress response, ...
Tópico(s): Bacterial biofilms and quorum sensing
2017 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Catarina Coelho, Hermı́nia de Lencastre, Marta Aires-de-Sousa,
Tópico(s): Bacterial biofilms and quorum sensing
2017 - Springer Science+Business Media | European Journal of Clinical Microbiology & Infectious Diseases
Ana Tavares, Maria Miragaia, Joana Rolo, Catarina Coelho, Hermı́nia de Lencastre, Ca-Mrsa,
Tópico(s): Antibiotic Use and Resistance
2013 - Springer Science+Business Media | European Journal of Clinical Microbiology & Infectious Diseases
Choon Kim, Michael Mwangi, Marilyn Chung, Catarina Milheirço, Hermı́nia de Lencastre, Alexander Tomasz,
All methicillin resistant S. aureus (MRSA) strains carry an acquired genetic determinant--mecA or mecC--which encode for a low affinity penicillin binding protein -PBP2A or PBP2A'--that can continue the catalysis of peptidoglycan transpeptidation in the presence of high concentrations of beta-lactam antibiotics which would inhibit the native PBPs normally involved with the synthesis of staphylococcal cell wall peptidoglycan. In contrast to this common genetic and biochemical mechanism carried by all ...
Tópico(s): Streptococcal Infections and Treatments
2013 - Public Library of Science | PLoS ONE
Sofia Fernandes, Daniela Proença, Cátia Cantante, Filipa Antunes Silva, Clara Leandro, Sara Lourenço, Catarina Milheiriço, Hermı́nia de Lencastre, Patrícia Cavaco‐Silva, Madalena Pimentel, Carlos São‐José,
Due to their bacterial lytic action, bacteriophage endolysins have recently gained great attention as a potential alternative to antibiotics in the combat of Gram-positive pathogenic bacteria, particularly those displaying multidrug resistance. However, large-scale production and purification of endolysins is frequently impaired due to their low solubility. In addition, a large number of endolysins appear to exhibit reduced lytic efficacy when compared with their action during phage infection. Here, ...
Tópico(s): Antimicrobial Resistance in Staphylococcus
2012 - Mary Ann Liebert, Inc. | Microbial Drug Resistance
Duarte C. Oliveira, Catarina Milheiriço, Hermı́nia de Lencastre,
Previously we identified a SCCmec variant similar in size to type IV but with a new ccrAB allotype, allotype 4. We addressed the epidemiological relevance of this variant and found it among several strains belonging to the same clone. We propose to rename this structural variant SCCmec type VI.
Tópico(s): Plant-Microbe Interactions and Immunity
2006 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Pedro Arêde, Catarina Milheiriço, Hermı́nia de Lencastre, Duarte C. Oliveira,
Methicillin-resistant Staphylococcus aureus (MRSA) is an important human pathogen, which is cross-resistant to virtually all β-lactam antibiotics. MRSA strains are defined by the presence of mecA gene. The transcription of mecA can be regulated by a sensor-inducer (MecR1) and a repressor (MecI), involving a unique series of proteolytic steps. The induction of mecA by MecR1 has been described as very inefficient and, as such, it is believed that optimal expression of β-lactam resistance by MRSA requires ...
Tópico(s): Bacterial Genetics and Biotechnology
2012 - Public Library of Science | PLoS Pathogens
Duarte C. Oliveira, Catarina Milheiriço, Susana Vinga, Hermı́nia de Lencastre,
Objectives: To evaluate the allelic variability of ccrA and ccrB among methicillin-resistant Staphylococcus aureus (MRSA) strains belonging to different genetic lineages and carrying different SCCmec types.
Tópico(s): Biochemical and Structural Characterization
2006 - Oxford University Press | Journal of Antimicrobial Chemotherapy
... exemplificarão com a obra de uma poetisa esquecida: Catarina de Lencastre, pretexto adicional para rever os cânones da nossa ...
Tópico(s): History of Colonial Brazil
2012 - Association Portugaise d'Etudes Françaises | Carnets
María Pardos de la Gándara, Vítor Borges, Marilyn Chung, Catarina Milheiriço, João Paulo Gomes, Hermı́nia de Lencastre, Alexander Tomasz,
Methicillin-resistant Staphylococcus aureus (MRSA) strains carry either a mecA- or a mecC-mediated mechanism of resistance to beta-lactam antibiotics, and the phenotypic expression of resistance shows extensive strain-to-strain variation. In recent communications, we identified the genetic determinants associated with the stringent stress response that play a major role in the antibiotic resistant phenotype of the historically earliest "archaic" clone of MRSA and in the mecC-carrying MRSA strain LGA251. ...
Tópico(s): Bacterial Identification and Susceptibility Testing
2018 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Teresa Conceição, Catarina Coelho, I. Santos Silva, Hermı́nia de Lencastre, Marta Aires-de-Sousa,
The aim of the present study was to determine the prevalence and risk factors for methicillin-resistant Staphylococcus aureus (MRSA) nasal carriage among patients and healthcare workers in Angola (ANG), São Tomé and Príncipe (STP), Cape Verde (CV) and East Timor (ET), and to characterize the antimicrobial susceptibility, virulence content and population structure of all S. aureus. Despite the importance of MRSA as a major human pathogen, data from these former Portuguese colonies in Africa and Asia are ...
Tópico(s): Bacterial biofilms and quorum sensing
2015 - Elsevier BV | Clinical Microbiology and Infection
Catarina Milheiriço, Duarte C. Oliveira, Hermı́nia de Lencastre,
Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.
Tópico(s): Mycobacterium research and diagnosis
2007 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Catarina Milheiriço, Duarte C. Oliveira, Hermı́nia de Lencastre,
ObjectivesTo develop and validate a new multiplex PCR strategy for subtyping SCCmec type IV methicillin-resistant Staphylococcus aureus (MRSA) strains—SCCmec IV multiplex PCR.
Tópico(s): Antibiotic Resistance in Bacteria
2007 - Oxford University Press | Journal of Antimicrobial Chemotherapy
Catarina Milheiriço, Ana Portelinha, Ludwig Krippahl, Hermı́nia de Lencastre, Duarte C. Oliveira,
Abstract Background The β-lactamase ( bla ) locus, which confers resistance to penicillins only, may control the transcription of mecA , the central element of methicillin resistance, which is embedded in a polymorphic heterelogous chromosomal cassette (the SCC mec element). In order to assess the eventual correlation between bla allotypes and genetic lineages, SCC mec types and/or β-lactam resistance phenotypes, the allelic variation on the bla locus was evaluated in a representative collection of 54 ...
Tópico(s): Bacterial biofilms and quorum sensing
2011 - BioMed Central | BMC Microbiology
João André Carriço, Catarina Silva-Costa, José Melo‐Cristino, Francisco Pinto, Hermı́nia de Lencastre, Jonas S. Almeida, Mário Ramirez,
ABSTRACT The studies that correlate the results obtained by different typing methodologies rely solely on qualitative comparisons of the groups defined by each methodology. We propose a framework of measures for the quantitative assessment of correspondences between different typing methods as a first step to the global mapping of type equivalences. A collection of 325 macrolide-resistant Streptococcus pyogenes isolates associated with pharyngitis cases in Portugal was used to benchmark the proposed ...
Tópico(s): Infective Endocarditis Diagnosis and Management
2006 - American Society for Microbiology | Journal of Clinical Microbiology
Catarina Milheiriço, Duarte C. Oliveira, Hermı́nia de Lencastre,
Tópico(s): Bauxite Residue and Utilization
2007 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Duarte C. Oliveira, Marcelino Santos, Catarina Milheiriço, João André Carriço, Susana Vinga, Arlindo L. Oliveira, Hermı́nia de Lencastre,
Sir, In 2006, we published a manuscript in this journal describing the allelic variation in the ccrAB locus in a representative collection of methicillin-resistant Staphylococcus aureus (MRSA) based on DNA sequencing of internal fragments of both genes. This work confirmed the very close relationships among ccrAB alleles associated with SCCmec types I–IV and VI, which was found to be independent of the MRSA lineage, geographic origin or isolation period. Moreover, particularly for the ccrB gene, ...
Tópico(s): Antifungal resistance and susceptibility
2008 - Oxford University Press | Journal of Antimicrobial Chemotherapy
Diana Espadinha, Rita G. Sobral, Catarina Inês Mendes, Guillaume Méric, Samuel K. Sheppard, João André Carriço, Hermínia de Lencastre, Maria Miragaia,
Background: Staphylococcus epidermidis is a common skin commensal that has emerged as a pathogen in hospitals, mainly related to medical devices-associated infections. Noteworthy, infection rates by S. epidermidis have the tendency to rise steeply in next decades together with medical devices use and immunocompromized population growth. S. epidermidis population structure includes two major clonal lineages (A/C and B) that present contrasting pathogenic potentials. To address this distinction and ...
Tópico(s): Bacterial Identification and Susceptibility Testing
2019 - Frontiers Media | Frontiers in Microbiology
Choon Kim, Catarina Milheiriço, Susana Gardete, Mark A. Holmes, Matthew T. G. Holden, Hermı́nia de Lencastre, Alexander Tomasz,
Methicillin-resistant Staphylococcus aureus (MRSA) strains show strain-to-strain variation in resistance level, in genetic background, and also in the structure of the chromosomal cassette (SCCmec) that carries the resistance gene mecA. In contrast, strain-to-strain variation in the sequence of the mecA determinant was found to be much more limited among MRSA isolates examined so far. The first exception to this came with the recent identification of MRSA strain LGA251, which carries a new homolog ...
Tópico(s): Bacterial biofilms and quorum sensing
2012 - Elsevier BV | Journal of Biological Chemistry
Produção Nacional
Revisado por pares
Juliana Lamaro‐Cardoso, Hermı́nia de Lencastre, André Kipnis, Fabiana Cristina Pimenta, Luciana S. C. Oliveira, Renato Maurício de Oliveira, Simonne S. Nouer, Marta Aires-de-Sousa, Catarina Milheiriço, Ana Lúcia S. Sampaio de Andrade,
ABSTRACT Investigations regarding Staphylococcus aureus carriage among Brazilian children are scarce. We evaluated the determinants of S. aureus and methicillin-resistant S. aureus (MRSA) nasal carriage in infants attending day care centers (DCCs) and the molecular features of the MRSA strains. A total of 1,192 children aged 2 months to 5 years attending 62 DCCs were screened for S. aureus and MRSA nasal carriage. MRSA isolates were characterized by pulsed-field gel electrophoresis, multilocus sequence ...
Tópico(s): Streptococcal Infections and Treatments
2009 - American Society for Microbiology | Journal of Clinical Microbiology
Catarina Milheiriço, Alexander Tomasz, Hermı́nia de Lencastre,
The acquisition of the resistance determinant mecA by Staphylococcus aureus is of major clinical importance, since it confers a resistant phenotype to virtually the entire large family of structurally diverse β-lactam antibiotics. While the common resistance determinant mecA is essential, the optimal expression of the resistance phenotype also requires additional factors. Previous studies showed that the great majority of clinical isolates of methicillin-resistant S. aureus (MRSA) have a heterogeneous ...
Tópico(s): Microbial Natural Products and Biosynthesis
2022 - Multidisciplinary Digital Publishing Institute | Antibiotics
Sofia Félix, Sara Handem, Sónia Nunes, Ana Cristina Paulo, Catarina Candeias, Carina Valente, Alexandra S. Simões, Antonio Almeida Silva, Débora A. Tavares, António Brito‐Avô, Hermı́nia de Lencastre, Raquel Sá‐Leão,
In Portugal, the 13-valent pneumococcal conjugate vaccine (PCV13) was commercially available between 2010 and 2015, following a decade of private use of PCV7. We evaluated changes on serotype distribution and antimicrobial susceptibility of pneumococci carried by children living in two regions of Portugal (one urban and one rural). Three epidemiological periods were defined: pre-PCV13 (2009-2010), early-PCV13 (2011-2012), and late-PCV13 (2015-2016). Nasopharyngeal samples (n = 4,232) were obtained from ...
Tópico(s): Pneumocystis jirovecii pneumonia detection and treatment
2021 - Elsevier BV | Vaccine
Catarina Milheiriço, Oliveira Dc, de Lencastre H,
Tópico(s): Medical Device Sterilization and Disinfection
2007 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Ana Catarina Reis, Leonor Lencastre, Marina Prista Guerra, Eduardo Remor,
O presente estudo observou a existência de relações significativas entre sintomatologia psicopatológica (BSI), adesão ao tratamento (CEAT-VIH) e qualidade de vida (WHOQOL-Bref) em 125 pessoas HIV-positivo/AIDS em tratamento antirretroviral, atendidos em um hospital do Porto (Portugal). A sintomatologia psicopatológica correlacionou-se negativamente com adesão ao tratamento e qualidade de vida, e a adesão ao tratamento associou-se positivamente com a qualidade de vida. O modelo preditor de adesão ao ...
Tópico(s): Adolescent Sexual and Reproductive Health
2010 - Springer Science+Business Media | Psicologia Reflexão e Crítica
Correia Garção, Domingos dos Reis Quita, José Basílio da Gama, Antônio Dinis da Cruz e Silva, Cláudio Manuel da Costa, Inácio José de Alvarenga Peixoto, Manuel Inácio da Silva Alvarenga, Teresa de Melo Breyner, Nicolau Tolentino de Almeida, Catarina de Lencastre, Manuel Maria Barbosa du Bocage, Alberto Magalhães, Simões Dias, Guerra Junqueiro, Machado de Assis, José Branquinho,
Antologia de poemas (1759-1905).
Tópico(s): Literature, Culture, and Criticism
2021 - | Teresa
Catarina Gouveia, Alexandra Gavino, Ons Bouchami, Maria Miragaia, Luís Varandas, Hermı́nia de Lencastre, Maria João Brito,
Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emerging public health problem worldwide. Severe invasive infections have been described, mostly associated with the presence of Panton-Valentine leukocidin (PVL). In Portugal limited information exists regarding CA-MRSA infections. In this study we describe the case of a previously healthy 12-year-old female, sport athlete, who presented to the hospital with acetabulofemoral septic arthritis, myositis, fasciitis, acetabulum ...
Tópico(s): Infective Endocarditis Diagnosis and Management
2013 - Hindawi Publishing Corporation | Case Reports in Pediatrics
María Pardos de la Gándara, Vítor Borges, Marilyn Chung, Catarina Milheiriço, João Paulo Gomes, Hermı́nia de Lencastre, Alexander Tomasz,
Volume 62, no. 6, e00206-18, 2018, [https://doi.org/10.1128/AAC.00206-18][1]. The authors note that (i) the gene that was affected in this study, encoding the ribose-phosphate pyrophosphokinase, is associated with different gene designations ( prs or prsA ) in genome annotations of different
Tópico(s): Oral microbiology and periodontitis research
2018 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Antonio Almeida Silva, Ana Cristina Paulo, João Babo, João Borralho, Catarina Figueiredo, Bruno Gonçalves, João Lança, Mónica Louro, Hermes Morais, Joana Queiroz, Hermı́nia de Lencastre, Raquel Sá‐Leão,
Methicillin-resistant Staphylococcus aureus (MRSA) has long been known as a major cause of hospital-acquired (HA-MRSA) infections worldwide. For the past twenty years, an increasing number of studies have described its emergence in the community as well. In Portugal, a country with a high-prevalence of HA-MRSA, there are only limited data available on the epidemiology of MRSA in the community. We studied the prevalence of S . aureus and MRSA colonization among healthy adults in Portugal. Between February ...
Tópico(s): Infective Endocarditis Diagnosis and Management
2021 - Public Library of Science | PLoS ONE
Catarina Candeias, Sofia Félix, Sara Handem, Hermı́nia de Lencastre, Raquel Sá‐Leão,
In Portugal, the 13-valent pneumococcal conjugate vaccine (PCV13) was available for private use from 2010 to 2015 and it was introduced in the National Immunization Program in 2015. We have reported that private use of PCV13 led to extensive serotype replacement and an increase in antimicrobial susceptibility among pneumococci carried by healthy children. We investigated which clonal changes concurred with these observations. A total of 657 pneumococcal strains, representative of a collection of ...
Tópico(s): Pneumocystis jirovecii pneumonia detection and treatment
2023 - American Society for Microbiology | Microbiology Spectrum