Julia Kazmierski, Kirstin Friedmann, Dylan Postmus, Jackson Emanuel, Cornelius Fischer, Jenny Jansen, Anja Richter, Laure Bosquillon de Jarcy, Christiane Schüler, Madlen Sohn, Sascha Sauer, Christian Drosten, Antoine‐Emmanuel Saliba, Leif Erik Sander, Marcel A. Müller, Daniela Niemeyer, Christine Goffinet,
... Search for more papers by this author Christian Drosten Christian Drosten Institute of Virology, Campus Charité Mitte, Charité – ... Search for more papers by this author Christian Drosten Christian Drosten Institute of Virology, Campus Charité Mitte, Charité – Universitätsmedizin ...
Tópico(s): Long-Term Effects of COVID-19
2022 - Springer Nature | Molecular Systems Biology
David P. Tchouassi, Marco Marklewitz, Edith Chepkorir, Florian Zirkel, Sheila B. Agha, Caroline Tigoi, Edith Koskei, Christian Drosten, Christian Borgemeister, Baldwyn Torto, Sandra Junglen, Rosemary Sang,
Abstract We describe a novel virus, designated Ntepes virus (NPV), isolated from sand flies in Kenya. NPV has the characteristic phlebovirus trisegmented genome architecture and is related to, but distinct from, Gabek Forest phlebovirus. Diverse cell cultures derived from wildlife, livestock, and humans were susceptible to NPV, with pronounced permissiveness in swine and rodent cells. NPV infection of newborn mice caused rapid and fatal illness. Permissiveness for NPV replication in sand fly cells, ...
Tópico(s): Vector-borne infectious diseases
2019 - Centers for Disease Control and Prevention | Emerging infectious diseases
Houriiyah Tegally, James Emmanuel San, Matthew Cotten, Monika Moir, Bryan Tegomoh, Gerald Mboowa, Darren P. Martin, Cheryl Baxter, Arnold W. Lambisia, Amadou Diallo, Daniel G. Amoako, Moussa Moïse Diagne, Abay Sisay, Abdel‐Rahman N. Zekri, Abdou Salam Guèye, Abdoul Karim Sangaré, Abdoul–Salam Ouédraogo, Abdourahmane Sow, Abdualmoniem O. Musa, Abdul Karim Sesay, Abe G. Abias, Adam Elzagheid, Adamou Lagaré, Adedotun-Sulaiman Kemi, Aden Elmi Abar, Adeniji A. Johnson, Adeola Fowotade, Oluwapelumi O. Adeyemi, Adrienne Amuri-Aziza, Agnes Juru, Ahmed Kandeil, Ahmed Mostafa, Ahmed Rebaï, Ahmed Sayed, Kazeem Akano, Aladje Baldé, Alan Christoffels, Alexander J. Trotter, Allan Campbell, Alpha Kabinet Keïta, A. Koné, Amal Bouzid, Amal Souissi, Ambrose Agweyu, Amel Naguib, Ana Victoria Gutiérrez, Anatole Nkeshimana, Andrew J. Page, Anges Yadouléton, Anika Vinzé, Anise N. Happi, Anissa Chouikha, Arash Iranzadeh, Arisha Maharaj, Armel L. Batchi‐Bouyou, Arshad Ismail, Augustina Angelina Sylverken, Augustine Goba, Femi Ayoade, Ayotunde E. Sijuwola, Baba M, Babatunde Lawal Salako, Bamidele Soji Oderinde, Bankole Bolajoko, Bassirou Diarra, Belinda L. Herring, Benjamin Tsofa, Bernard Lekana-Douki, Bernard Mvula, Berthe‐Marie Njanpop‐Lafourcade, Blessing T. Marondera, Bouh Abdi Khaireh, Bouréma Kouriba, Bright Adu, Brigitte Pool, Bronwyn McInnis, Cara E. Brook, Carolyn Williamson, Cassien Nduwimana, Catherine Anscombe, Catherine Pratt, Cathrine Scheepers, Chantal Akoua‐Koffi, Charles N. Agoti, Chastel M. Mapanguy, Cheikh Loucoubar, Chika Onwuamah, Chikwe Ihekweazu, Christian Malaka, Christophe N. Peyrefitte, Chukwa Grace, Chukwuma Ewean Omoruyi, Clotaire Donatien Rafaï, Collins M. Morang’a, Cyril Erameh, Daniel Lule Bugembe, Daniel J. Bridges, Daniel Mukadi‐Bamuleka, Daniel J. Park, David A. Rasmussen, David Baker, D. James Nokes, Deogratius Ssemwanga, Derek Tshiabuila, Dominic S. Y. Amuzu, Dominique Goedhals, Donald S. Grant, Donwilliams O. Omuoyo, Dorcas Maruapula, Dorcas Wanjohi, Ebenezer Foster-Nyarko, Eddy Kinganda-Lusamaki, Edgar Simulundu, Edidah M. Ong’era, Edith N. Ngabana, Edward Okoth Abworo, Edward Otieno, Edwin Shumba, Edwine Barasa, El Bara Ahmed, Elhadi Abdalla Ahmed, Emmanuel Lokilo, Enatha Mukantwari, Eromon Philomena, Essia Belarbi, Etienne Simon‐Lorière, Etilé A. Anoh, Eusébio Manuel, Fabian H. Leendertz, Fahn Taweh, Wasfi Farès, Fatma Abdelmoula, Faustinos Tatenda Takawira, Fawzi Derrar, Fehintola V. Ajogbasile, Florette K. Treurnicht, Onikepe Folarin, Francine Ntoumi, Francisca Muyembe, Frank Edgard Zongo Ragomzingba, Fred Athanasius Dratibi, Fred-Akintunwa Iyanu, Gabriel K. Mbunsu, Gaetän Thilliez, Gemma L. Kay, George O. Akpede, Gert U. van Zyl, Gordon A. Awandare, Grace Kpeli, Grit Schubert, Gugu Maphalala, Hafaliana C. Ranaivoson, Hannah E. Omunakwe, Harris Onywera, Haruka Abe, Héla Karray, Hellen Nansumba, Henda Triki, Herve Albéric Adje Kadjo, Hesham A. Elgahzaly, Hlanai Gumbo, Hota Mathieu, Hugo Kavunga‐Membo, Ibtihel Smeti, Idowu B. Olawoye, Ifedayo Adetifa, Ikponmwosa Odia, Ilhem Boutiba Ben Boubaker, Iluoreh Ahmed Muhammad, Isaac Ssewanyana, Isatta Wurie, Iyaloo Konstantinus, Jacqueline Wemboo Afiwa Halatoko, James Ayei, Janaki Sonoo, Jean-Claude Makangara-Cigolo, Jean‐Jacques Muyembe Tamfum, Jean‐Michel Héraud, Jeffrey G. Shaffer, Jennifer Giandhari, Jennifer Musyoki, Jerome Nkurunziza, Jessica N. Uwanibe, Jinal N. Bhiman, Jiro Yasuda, Joana Morais, Jocelyn Kiconco, John Demby Sandi, John Huddleston, John Kofi Odoom, John Mwita Morobe, John O. Gyapong, John Kayiwa, Johnson Okolie, Joicymara S. Xavier, Jones Gyamfi, Joseph Francis Wamala, Joseph Humphrey Kofi Bonney, Joseph Nyandwi, Josie Everatt, Joweria Nakaseegu, Joyce M. Ngoi, Joyce Namulondo, Judith U. Oguzie, Julia C. Andeko, Julius J. Lutwama, Juma J. H. Mogga, Justin O’Grady, Katherine J. Siddle, Kathleen Victoir, Kayode T. Adeyemi, Kefentse A. Tumedi, Kevin Sanders Da Rosa Carvalho, Khadija Said Mohammed, Koussay Dellagi, Kunda Musonda, Kwabena Obeng Duedu, Lamia Fki‐Berrajah, Lavanya Singh, Lenora Kepler, Leon Biscornet, Leonardo de Oliveira Martins, Lucious Chabuka, Luicer A. Ingasia, Lul Deng Ojok, Lul Lojok Deng, Lynette Isabella Ochola‐Oyier, Lynn Tyers, Madisa Mine, Magalutcheemee Ramuth, Maha Mastouri, Mahmoud ElHefnawi, Maïmouna Mbanne, M.I. Matsheka, Malebogo Kebabonye, Mamadou Diop, Mambu Momoh, Maria da Luz Lima Mendonça, Marietjie Venter, Mariétou F Paye, Martin Faye, Martin M. Nyaga, Mathabo Mareka, Damaris Matoke-Muhia, Maureen W. Mburu, Maxmillian Mpina, Michael Owusu, Michael R. Wiley, Mirabeau Youtchou Tatfeng, Mitoha Ondo’o Ayekaba, Mohamed Abouelhoda, Mohamed Amine Beloufa, Mohamed G. Seadawy, Mohamed K. Khalifa, Mooko Marethabile Matobo, Mouhamed Kane, Mounérou Salou, Mphaphi B. Mbulawa, Mulenga Mwenda, Mushal Allam, My V. T. Phan, Nabil Abid, Nadine Rujeni, Nadir Abuzaid, Nália Ismael, Nancy Elguindy, Ndeye Marième Top, Ndongo Dia, Nédio Mabunda, Marvin Hsiao, Nelson Boricó Silochi, Ngiambudulu M. Francisco, Ngonda Saasa, Nicholas Bbosa, Nickson Murunga, Nicksy Gumede, Nicole Wolter, Nikita Sitharam, Nnaemeka Ndodo, Nnennaya Anthony Ajayi, Noël Tordo, Nokuzola Mbhele, Norosoa Harline Razanajatovo, Nosamiefan Iguosadolo, Nwando Mba, Ojide C. Kingsley, Okogbenin Sylvanus, Femi Oladiji, Moses Olubusuyi Adewumi, Testimony Olumade, Olusola Ogunsanya, Oluwatosin Fakayode, Onwe Emeka Ogah, Ope-Ewe Oludayo, Ousmane Faye, Pamela Smith-Lawrence,
Investment in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing in Africa over the past year has led to a major increase in the number of sequences that have been generated and used to track the pandemic on the continent, a number that now exceeds 100,000 genomes. Our results show an increase in the number of African countries that are able to sequence domestically and highlight that local sequencing enables faster turnaround times and more-regular routine surveillance. Despite ...
American Association for the Advancement of Science
Anna Maria Eis‐Hübinger, Jan Felix Drexler, Ulrike Reber, Marcus Panning, Christian Drosten, Marijke Weber‐Schehl, Doris Hedges, Sally A. Baylis, W. Kurt Roth,
... Freiburg, GermanySearch for more papers by this authorChristian Drosten MD, Prof., Christian Drosten MD, Prof. Institute of VirologyUniversity Medical Centre ... Freiburg, GermanySearch for more papers by this authorChristian Drosten MD, Prof., Christian Drosten MD, Prof. Institute of VirologyUniversity Medical Centre BonnBonn, ...
Tópico(s): Blood groups and transfusion
2009 - Wiley | Transfusion
Saskia Trump, Soeren Lukassen, Markus S. Anker, Robert Lorenz Chua, Johannes Liebig, Loreen Thürmann, Victor M. Corman, Marco Binder, Jennifer Loske, Christina Klasa, Teresa G. Krieger, Bianca P. Hennig, Marey Messingschlager, Fabian Pott, Julia Kazmierski, Sven Twardziok, Jan Philipp Albrecht, Roland Eils, Sara Hadzibegovic, Alessia Lena, Bettina Heidecker, Thore Bürgel, Jakob Steinfeldt, Christine Goffinet, Florian Kurth, Martin Witzenrath, Maria Theresa Völker, Sarah Dorothea Müller, Uwe G. Liebert, Naveed Ishaque, Lars Kaderali, Leif Erik Sander, Christian Drosten, Sven Laudi, Roland Eils, Christian Conrad, Ulf Landmesser, Irina Lehmann,
In coronavirus disease 2019 (COVID-19), hypertension and cardiovascular diseases are major risk factors for critical disease progression. However, the underlying causes and the effects of the main anti-hypertensive therapies—angiotensin-converting enzyme inhibitors (ACEIs) and angiotensin receptor blockers (ARBs)—remain unclear. Combining clinical data (n = 144) and single-cell sequencing data of airway samples (n = 48) with in vitro experiments, we observed a distinct inflammatory predisposition of immune ...
Tópico(s): SARS-CoV-2 and COVID-19 Research
2020 - Nature Portfolio | Nature Biotechnology
Robert Lorenz Chua, Soeren Lukassen, Saskia Trump, Bianca P. Hennig, Daniel Wendisch, Fabian Pott, Olivia Debnath, Loreen Thürmann, Florian Kurth, Maria Theresa Völker, Julia Kazmierski, Bernd Timmermann, Sven Twardziok, Stefan Schneider, Felix Machleidt, Holger Müller-Redetzky, Melanie Maier, Alexander Krannich, Sein Schmidt, Felix Balzer, Johannes Liebig, Jennifer Loske, Norbert Suttorp, Roland Eils, Naveed Ishaque, Uwe G. Liebert, Christof von Kalle, Andreas C. Hocke, Martin Witzenrath, Christine Goffinet, Christian Drosten, Sven Laudi, Irina Lehmann, Christian Conrad, Leif Erik Sander, Roland Eils,
To investigate the immune response and mechanisms associated with severe coronavirus disease 2019 (COVID-19), we performed single-cell RNA sequencing on nasopharyngeal and bronchial samples from 19 clinically well-characterized patients with moderate or critical disease and from five healthy controls. We identified airway epithelial cell types and states vulnerable to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In patients with COVID-19, epithelial cells showed an average ...
Tópico(s): Respiratory viral infections research
2020 - Nature Portfolio | Nature Biotechnology
Laura Esposito, Jan Felix Drexler, Oliver Braganza, Elke Doberentz, Alexander Grote, Guido Widman, Christian Drosten, Anna M. Eis‐Hübinger, Susanne Schoch, Christian E. Elger, Albert J. Becker, Pitt Niehusmann,
Summary Objective Chronic inflammatory processes are important promotors of temporal lobe epilepsy (TLE) development. Based on human herpesvirus 6 (HHV‐6) DNA detection in brain tissue from patients with TLE, an association of persistent viral infection with TLE has been discussed. Individual studies reported increased HHV‐6 DNA in patients with clinical signs of previous inflammatory brain reaction, that is, febrile seizures or meningoencephalitis. However, detection rates vary considerably between ...
Tópico(s): Infectious Encephalopathies and Encephalitis
2014 - Wiley | Epilepsia
Anne Seltmann, Victor M. Corman, Andrea Rasche, Christian Drosten, Gábor Á. Czirják, Henry Bernard, Matthew J. Struebig, Christian C. Voigt,
Emerging infectious diseases (EIDs) are considered a major threat to global health. Most EIDs appear to result from increased contact between wildlife and humans, especially when humans encroach into formerly pristine habitats. Habitat deterioration may also negatively affect the physiology and health of wildlife species, which may eventually lead to a higher susceptibility to infectious agents and/or increased shedding of the pathogens causing EIDs. Bats are known to host viruses closely related ...
Tópico(s): Viral Infections and Vectors
2017 - Springer Science+Business Media | EcoHealth
Christian Gortázar, Leslie A. Reperant, Thijs Kuiken, José de la Fuente, Mariana Boadella, Beatriz Martínez‐López, Francisco Ruiz‐Fons, Agustín Estrada‐Peña, Christian Drosten, Graham F. Medley, Richard S. Ostfeld, A. Townsend Peterson, Kurt C. VerCauteren, Christian Menge, Marc Artois, Constance Schultsz, Richard Delahay, Jordi Serra‐Cobo, Robert Poulin, Frédéric Keck, Alonso Aguirre, Heikki Henttonen, Andrew P. Dobson, Susan Kutz, Juan Lubroth, Atle Mysterud,
This research was funded by EU FP7 grant ANTIGONE (#278976). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Tópico(s): Animal Disease Management and Epidemiology
2014 - Public Library of Science | PLoS Pathogens
Bengisu Akbil, Tim Meyer, Paula Stubbemann, Charlotte Thibeault, Olga Staudacher, Daniela Niemeyer, Jenny Jansen, Barbara Mühlemann, Jan M. Doehn, Christoph Tabeling, Christian Nußhag, Cédric Hirzel, David Sökler Sanchez, Alexandra Nieters, Achim Lother, Daniel Duerschmied, Nils Schallner, Jan Nikolaus Lieberum, Dietrich August, Siegbert Rieg, Valeria Falcone, Hartmut Hengel, Uwe Kölsch, Nadine Unterwalder, Ralf‐Harto Hübner, Terry C. Jones, Norbert Suttorp, Christian Drosten, Klaus Warnatz, Thibaud Spinetti, Joerg C. Schefold, Thomas Dörner, Leif Erik Sander, Victor M. Corman, Uta Merle, Florian Kurth, Horst von Bernuth, Christian Meisel, Christine Goffinet,
Six to 19% of critically ill COVID-19 patients display circulating auto-antibodies against type I interferons (IFN-AABs). Here, we establish a clinically applicable strategy for early identification of IFN-AAB-positive patients for potential subsequent clinical interventions.
Tópico(s): Long-Term Effects of COVID-19
2022 - Springer Science+Business Media | Journal of Clinical Immunology
Daniel Wendisch, Oliver Dietrich, Tommaso Mari, Saskia von Stillfried, Ignacio L. Ibarra, Mirja Mittermaier, Christin Mache, Robert Lorenz Chua, Rainer Knoll, Sara Timm, Sophia Brumhard, Tobias Krammer, Henrik Zauber, Anna Luisa Hiller, Anna Pascual Reguant, Ronja Mothes, Roman D. Bülow, Jessica Schulze, Alexander M. Leipold, Sonja Djudjaj, Florian Erhard, Robert Geffers, Fabian Pott, Julia Kazmierski, Josefine Radke, Panagiotis Pergantis, Kevin Baßler, Claudia Conrad, Anna C. Aschenbrenner, Birgit Sawitzki, Markus Landthaler, Emanuel Wyler, David Horst, Stefan Hippenstiel, Andreas C. Hocke, Frank L. Heppner, Alexander Uhrig, Carmen García, Felix Machleidt, Susanne Herold, Sefer Elezkurtaj, Charlotte Thibeault, Martin Witzenrath, Clément Cochain, Norbert Suttorp, Christian Drosten, Christine Goffinet, Florian Kurth, Joachim L. Schultze, Helena Radbruch, Matthias Ochs, Roland Eils, Holger Müller-Redetzky, Anja E. Hauser, Malte D. Luecken, Fabian J. Theis, Christian Conrad, Thorsten Wolff, Peter Boor, Matthias Selbach, Antoine‐Emmanuel Saliba, Leif Erik Sander,
COVID-19-induced "acute respiratory distress syndrome" (ARDS) is associated with prolonged respiratory failure and high mortality, but the mechanistic basis of lung injury remains incompletely understood. Here, we analyze pulmonary immune responses and lung pathology in two cohorts of patients with COVID-19 ARDS using functional single-cell genomics, immunohistology, and electron microscopy. We describe an accumulation of CD163-expressing monocyte-derived macrophages that acquired a profibrotic transcriptional ...
Tópico(s): COVID-19 Clinical Research Studies
2021 - Cell Press | Cell
Les C. Cwynar, Glen M. MacDonald,
... Thierno D. Dallo, Carine Ngoagouni, Jan Felix Drexler, Christian Drosten, Emmanuel R. Nakouné, Eric M. Leroy, Serge Morand, ...
Tópico(s): Forest ecology and management
1987 - University of Chicago Press | The American Naturalist
Matthias Niedrig, H. Zeller, I. Schuffenecker, Christian Drosten, Petra Emmerich, Leonid Rumer, Oliver Donoso-Mantke,
External quality assurance for serological detection of chikungunya virus infection was performed to assess the diagnostic quality of expert laboratories. Of 30 participants, only six correctly analysed all reference samples with their respective tests. Thirteen laboratories gave at least 85% correct results, and 11 laboratories 75% or less. IgM antibodies were detected less frequently than IgG antibodies (p <0.001). The study provides information on the quality of different serological tests and ...
Tópico(s): Malaria Research and Control
2009 - Elsevier BV | Clinical Microbiology and Infection
Marcus Panning, Rémi N. Charrel, Oliver Donoso Mantke, Olfert Landt, Matthias Niedrig, Christian Drosten,
A preformulated chikungunya virus real-time reverse transcription-PCR, quality-confirmed oligonucleotides, and noninfectious virus controls were distributed by the European Network for the Diagnosis of Imported Viral Diseases. An international proficiency study with 31 participants demonstrated that ad hoc implementation of molecular diagnostics was feasible and successful.
Tópico(s): Malaria Research and Control
2009 - Centers for Disease Control and Prevention | Emerging infectious diseases
Produção Nacional
Revisado por pares
Jan Felix Drexler, Klaus Grywna, Andreas Stöcker, Patrícia S. Vilas Boas de Almeida, Tereza Cristina Medrado Ribeiro, Monika Eschbach-Bludau, Nadine Petersen, Hugo da Costa Ribeiro, Christian Drosten,
Abstract Human parechoviruses (HPeVs) were detected by reverse transcription–PCR in 16.1% of 335 stool samples from children <6 years of age with enteritis in Salvador, Brazil. Whole genome sequencing of 1 sample showed a novel HPeV that has been designated as HPeV8.
Tópico(s): Animal Virus Infections Studies
2009 - Centers for Disease Control and Prevention | Emerging infectious diseases
Ilona Glowacka, Stephanie Bertram, Petra Herzog, Susanne Pfefferle, Imke Steffen, Marcus O. Muench, Graham Simmons, Heike Hofmann, Thomas Kuri, Friedemann Weber, Jutta Eichler, Christian Drosten, Stefan Pöhlmann,
The human coronaviruses (CoVs) severe acute respiratory syndrome (SARS)-CoV and NL63 employ angiotensin-converting enzyme 2 (ACE2) for cell entry. It was shown that recombinant SARS-CoV spike protein (SARS-S) downregulates ACE2 expression and thereby promotes lung injury. Whether NL63-S exerts a similar activity is yet unknown. We found that recombinant SARS-S bound to ACE2 and induced ACE2 shedding with higher efficiency than NL63-S. Shedding most likely accounted for the previously observed ACE2 downregulation ...
Tópico(s): Long-Term Effects of COVID-19
2009 - American Society for Microbiology | Journal of Virology
Stefanie Kramme, Le An, Khoa Nguyen, Le Van Trin, Egbert Tannich, Jan Rybniker, Bernhard Fleischer, Christian Drosten, Marcus Panning,
Scrub typhus, caused by the intracellular bacterium Orientia tsutsugamushi, is a major cause of febrile illness in the Asia/Pacific region. Here, we implemented a novel real-time PCR and determined the relation of DNA target gene concentration with serum cytokine levels. The limit of detection of the novel real-time PCR was 1,062 DNA copies per ml of EDTA whole blood. Specificity was excellent as determined on a panel of blood- and skin-borne bacteria, including Rickettsia spp. as well as healthy ...
Tópico(s): Viral Infections and Vectors
2009 - American Society for Microbiology | Journal of Clinical Microbiology
... Johannes Blumel Prof. Dr. Reinhard Burger Prof. Dr. Christian Drosten Dr. Albrecht Groner Prof. Dr. Lutz Gurtler Dr. ...
Tópico(s): Hepatitis C virus research
2009 - Karger Publishers | Transfusion Medicine and Hemotherapy
Jabbar S. Ahmed, Michèle Bouloy, Önder Ergönül, Anthony R. Fooks, Janusz T. Pawęska, Alì Mirazimi, Christian Drosten, R.J.M. Moormann, Noël Tordo, Zati Vatansever, Paolo Calistri, Agustín Estrada‐Peña, Alì Mirazimi, Hermann Unger, Hong Yin, Ulrike Seitzer,
Arboviruses are arthropod-borne viruses, which include West Nile fever virus (WNFV), a mosquito-borne virus, Rift Valley fever virus (RVFV), a mosquito-borne virus, and Crimean-Congo haemorrhagic fever virus (CCHFV), a tick-borne virus. These arthropod-borne viruses can cause disease in different domestic and wild animals and in humans, posing a threat to public health because of their epidemic and zoonotic potential. In recent decades, the geographical distribution of these diseases has expanded. ...
Tópico(s): Energy and Environment Impacts
2009 - European Centre for Disease Prevention and Control | Eurosurveillance
Dominic Wichmann, Marcus Panning, Thomas Quack, Stefanie Kramme, Gerd‐Dieter Burchard, Christoph G. Grevelding, Christian Drosten,
Introduction Schistosomiasis (bilharzia), one of the most relevant parasitoses of humans, is confirmed by microscopic detection of eggs in stool, urine, or organ biopsies. The sensitivity of these procedures is variable due to fluctuation of egg shedding. Serological tests on the other hand do not distinguish between active and past disease. In patients with acute disease (Katayama syndrome), both serology and direct detection may produce false negative results. To overcome these obstacles, we developed ...
Tópico(s): Global Maternal and Child Health
2009 - Public Library of Science | PLoS neglected tropical diseases
Susanne Pfefferle, Samuel Oppong, Jan Felix Drexler, Florian Gloza‐Rausch, Anne Ipsen, Antje Seebens, Marcel A. Müller, Augustina Annan, Peter Vallo, Yaw Adu‐Sarkodie, Thomas Kruppa, Christian Drosten,
Abstract We tested 12 bat species in Ghana for coronavirus (CoV) RNA. The virus prevalence in insectivorous bats (n = 123) was 9.76%. CoV was not detected in 212 fecal samples from Eidolon helvum fruit bats. Leaf-nosed bats pertaining to Hipposideros ruber by morphology had group 1 and group 2 CoVs. Virus concentrations were <45,000 copies/100 mg of bat feces. The diversified group 1 CoV shared a common ancestor with the human common cold virus hCoV-229E but not with hCoV-NL63, disputing hypotheses of ...
Tópico(s): COVID-19 epidemiological studies
2009 - Centers for Disease Control and Prevention | Emerging infectious diseases
Anke Edelmann, Ulrich Kalus, Anke Oltmann, Angela Stein, Anett Unbehaun, Christian Drosten, Detlev H. Krüger, Jörg Hofmann,
BACKGROUND: Human immunodeficiency virus Type 1 (HIV‐1) assays applying nucleic acid testing (NAT) rely on HIV‐1 sequence‐specific primers and probes. Their hybridization can be limited or abolished by genetic polymorphisms occurring in the target sequence. STUDY DESIGN AND METHODS: Blood donations are routinely tested for HIV‐1/2 antibodies and for HIV‐1 RNA in our blood transfusion unit. Recently, HIV‐1 RNA was undetectable with an established in‐house real‐time long terminal repeat (LTR) reverse ...
Tópico(s): HIV/AIDS Research and Interventions
2009 - Wiley | Transfusion
M. Kariuki Njenga, Janusz T. Pawęska, Rose Wanjala, Carol Y. Rao, Matthew Weiner, Victor Omballa, Elizabeth T. Luman, David Mutonga, Shanaaz Sharif, Marcus Panning, Christian Drosten, Daniel R. Feikin, Robert F. Breiman,
Approximately 8% of Rift Valley fever (RVF) cases develop severe disease, leading to hemorrhage, hepatitis, and/or encephalitis and resulting in up to 50% of deaths. A major obstacle in the management of RVF and other viral hemorrhagic fever cases in outbreaks that occur in rural settings is the inability to rapidly identify such cases, with poor prognosis early enough to allow for more-aggressive therapies. During an RVF outbreak in Kenya in 2006 to 2007, we evaluated whether quantitative real-time ...
Tópico(s): Fire effects on ecosystems
2009 - American Society for Microbiology | Journal of Clinical Microbiology
Roman Wölfel, Janusz T. Pawęska, Nadine Petersen, Antoinette A. Grobbelaar, Patricia A. Leman, Roger Hewson, Marie‐Claude Georges‐Courbot, Anna Papa, Volker Heiser, Marcus Panning, Stephan Günther, Christian Drosten,
Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral zoonosis which occurs throughout Africa, Eastern Europe, and Asia and results in an approximately 30% fatality rate. A reverse transcription-PCR assay including a competitive internal control was developed on the basis of the most up-to-date genome information. Biotinylated amplification products were hybridized to DNA macroarrays on the surfaces of polymer supports, and hybridization events were visualized by incubation with a streptavidin- ...
Tópico(s): Vector-Borne Animal Diseases
2009 - American Society for Microbiology | Journal of Clinical Microbiology
Daniel Voß, Susanne Pfefferle, Christian Drosten, Lea Stevermann, Elisabetta Traggiai, Antonio Lanzavecchia, Stephan Becker,
The glycosylated membrane protein M of the severe acute respiratory syndrome associated coronavirus (SARS-CoV) is the main structural component of the virion and mediates assembly and budding of viral particles. The membrane topology of SARS-CoV M and the functional significance of its N-glycosylation are not completely understood as is its interaction with the surface glycoprotein S. Using biochemical and immunofluorescence analyses we found that M consists of a short glycosylated N-terminal ectodomain, ...
Tópico(s): Viral gastroenteritis research and epidemiology
2009 - BioMed Central | Virology Journal
Jan Felix Drexler, Angelika Helmer, Heike Kirberg, Ulrike Reber, Marcus Panning, Marcel A. Müller, Katja Höfling, B. Matz, Christian Drosten, Anna Maria Eis‐Hübinger,
Influenza A pandemic (H1N1) 2009 virus RNA was detected by reverse transcription-PCR in 144 clinical samples from Bonn, Germany. A common rapid antigen-based test detected the virus in only 11.1% of these samples. The paramount feature of rapid test-positive samples was high virus concentration. Antigen-based rapid tests appear unsuitable for virologic diagnostics in the current pandemic.
Tópico(s): Viral gastroenteritis research and epidemiology
2009 - Centers for Disease Control and Prevention | Emerging infectious diseases
Marcus Panning, Markus Heß, Waldemar Fischer, Klaus Grywna, Martin Pfeffer, Christian Drosten,
A novel commercial Chikungunya virus real-time reverse transcription-PCR (RT-PCR) kit was evaluated on a comprehensive panel of original patient samples. The assay was 100% sensitive and specific in comparison to a published real-time RT-PCR. Viral loads from both assays were highly correlated. The kit proved to be suitable for routine use in patient care.
Tópico(s): Viral Infections and Vectors
2009 - American Society for Microbiology | Journal of Clinical Microbiology
Susanne Pfefferle, Verena Krähling, Vanessa Ditt, Klaus Grywna, Elke Mühlberger, Christian Drosten,
Abstract During the outbreak of SARS in 2002/3, a prototype virus was isolated from a patient in Frankfurt/Germany (strain Frankfurt-1). As opposed to all other SARS-Coronavirus strains, Frankfurt-1 has a 45-nucleotide deletion in the transmembrane domain of its ORF 7b protein. When over-expressed in HEK 293 cells, the full-length protein but not the variant with the deletion caused interferon beta induction and cleavage of procaspase 3. To study the role of ORF 7b in the context of virus replication, ...
Tópico(s): Viral gastroenteritis research and epidemiology
2009 - BioMed Central | Virology Journal
Marcus Panning, Markus Eickmann, Olfert Landt, Masyar Monazahian, Stephan Ölschläger, Sigrid Baumgarte, Udo Reischl, Jürgen J. Wenzel, Hans Helmut Niller, Stephan Günther, B. Hollmann, Daniela Huzly, Jan Felix Drexler, Angelika Helmer, Stephan Becker, B. Matz, Anna Maria Eis‐Hübinger, Christian Drosten,
Influenza A(H1N1)v virus was first identified in April 2009. A novel real-time RT-PCR for influenza A(H1N1)v virus was set up ad hoc and validated following industry-standard criteria. The lower limit of detection of the assay was 384 copies of viral RNA per ml of viral transport medium (95% confidence interval: 273-876 RNA copies/ml). Specificity was 100% as assessed on a panel of reference samples including seasonal human influenza A virus H1N1 and H3N2, highly pathogenic avian influenza A virus ...
Tópico(s): Virus-based gene therapy research
2009 - European Centre for Disease Prevention and Control | Eurosurveillance
Produção Nacional
Revisado por pares
Jan Felix Drexler, Bernd Kupfer, Nadine Petersen, Rejane Maria Tommasini Grotto, Silvia Maria Corvino Rodrigues, Klaus Grywna, Marcus Panning, Augustina Annan, Giovanni Faria Silva, Jill Douglas, Evelyn Siew-Chuan Koay, Heidi Smuts, Eduardo Martins Netto, Peter Simmonds, Maria Inês de Moura Campos Pardini, W. Kurt Roth, Christian Drosten,
Detection and quantification of hepatitis C virus (HCV) RNA is integral to diagnostic and therapeutic regimens. All molecular assays target the viral 5'-noncoding region (5'-NCR), and all show genotype-dependent variation of sensitivities and viral load results. Non-western HCV genotypes have been under-represented in evaluation studies. An alternative diagnostic target region within the HCV genome could facilitate a new generation of assays.In this study we determined by de novo sequencing that the ...
Tópico(s): Hepatitis B Virus Studies
2009 - Public Library of Science | PLoS Medicine