Roza Wojcik, Kristian E. Swearingen, Jane A. Dickerson, Emily H. Turner, Lauren M. Ramsay, Norman J. Dovic̀hi,
3-(2-Furoyl)quinoline-2-carboxaldehyde (FQ), Chromeo P465, and Chromeo P503 are weakly fluorescent reagents that react with primary amines to produce fluorescent products. We studied the reaction of these ... was an order of magnitude faster with the Chromeo reagents than with FQ; however, the steady-state ... of two higher for FQ compared with the Chromeo reagents. The relative abundance of the products with ... contrast, the distribution of reaction products with the Chromeo reagents did not follow a binomial distribution for ...
Tópico(s): Click Chemistry and Applications
2008 - Elsevier BV | Journal of Chromatography A
Stephanie de Jong, Sergey N. Krylov,
... Here we suggest that protein labeling with new Chromeo dyes can help to overcome this obstacle. By labeling a number of proteins with Chromeo P503, we show that the labeling procedure enables ... mobility or their ability to bind DNA. Moreover, Chromeo P503 does not appear to label the amino- ... run buffers. Fluorescent labeling of protein targets with Chromeo dyes empowers selection of aptamers by KCE methods ...
Tópico(s): DNA and Nucleic Acid Chemistry
2011 - American Chemical Society | Analytical Chemistry
Emily H. Turner, Jane A. Dickerson, Lauren M. Ramsay, Kristian E. Swearingen, Roza Wojcik, Norman J. Dovic̀hi,
... spectroscopic and electrophoretic properties of proteins labeled with Chromeo P503 were investigated. Its photobleaching characteristics were determined by continually infusing Chromeo P503-labeled α-lactalbumin into a sheath-flow ... labeled protein. One-dimensional capillary electrophoresis analysis of Chromeo P503-labeled α-lactalbumin produced concentration detection limits ( ...
Tópico(s): Biosensors and Analytical Detection
2008 - Elsevier BV | Journal of Chromatography A
Matthew Geiger, Michael T. Bowser,
... and amino acids. Of the labeling reagents assessed, Chromeo P503 performed the best for nLC × μFFE separations of peptides. A nLC × μFFE analysis of a Chromeo P503-labeled BSA tryptic digest produced a 2D ... a 75 s separation window (76 peaks/min). Chromeo P503 and Alexa Fluor 488-labeled amino acids ...
Tópico(s): Mass Spectrometry Techniques and Applications
2016 - American Chemical Society | Analytical Chemistry
Sarah K. Anciaux, Michael T. Bowser,
... fluorescein. Surface adsorption was observed in separations of Chromeo P503 labeled myoglobin and cytochrome c but was ... s were observed for both proteins. For comparison, Chromeo P503 labeled myoglobin and cytochrome c adsorb strongly ... min. A 2D nLC × µFFE separation of a Chromeo P503 labeled tryptic digest of BSA was performed ...
Tópico(s): Microfluidic and Bio-sensing Technologies
2019 - Wiley | Electrophoresis
Lauren M. Ramsay, Jane A. Dickerson, Oluwatosin O. Dada, Norman J. Dovic̀hi,
... paper addresses these issues. For labeling, we employ Chromeo P540, which is a fluorogenic reagent that converts ... mass detection limits of 150 ± 15 zmol for Chromeo P540 labeled β-lactoglobulin. Concentration detection limits were ...
Tópico(s): Biosensors and Analytical Detection
2009 - American Chemical Society | Analytical Chemistry
Lauren M. Ramsay, Jane A. Dickerson, Norman J. Dovic̀hi,
... a post‐column sheath flow cuvette. We employed Chromeo P503 as a fluorogenic reagent to label proteins ... in a 40 min separation. Detection limits for Chromeo P503‐labeled β ‐lactoglobulin were 5 amol injected ...
Tópico(s): Mass Spectrometry Techniques and Applications
2009 - Wiley | Electrophoresis
Kristian E. Swearingen, Jane A. Dickerson, Emily H. Turner, Lauren M. Ramsay, Roza Wojcik, Norman J. Dovic̀hi,
The fluorogenic reagent Chromeo P465 is considered for the analysis of proteins by capillary electrophoresis with laser-induced fluorescence detection. The reagent was ... α-chymotrypsinogen A, and α-lactalbumin labeled with Chromeo P465; unfortunately, baseline resolution was not achieved with ...
Tópico(s): Mass Spectrometry Techniques and Applications
2008 - Elsevier BV | Journal of Chromatography A
Mei He, Hiroshi Kubo, Konosuke Morimoto, Naoya Fujino, Takaya Suzuki, Toru Takahasi, Mitsuhiro Yamada, Mutsuo Yamaya, T Maekawa, Yasuhiko Yamamoto, Hiroshi Yamamoto,
... nitro-2-1,3-benzoxadiazol-4-yl)) and Chromeo 546-labelled sRAGE. The distribution of phosphatidylserine and ... appeared on the cell surface, with the acceptor (Chromeo 546–sRAGE, red fluorescence) being photobleached. By contrast, ...
Tópico(s): Advanced Glycation End Products research
2011 - Springer Nature | EMBO Reports
Jane A. Dickerson, Lauren M. Ramsay, Oluwatosin O. Dada, Nathan Cermak, Norman J. Dovic̀hi,
... potential. Proteins are labeled with the fluorogenic reagent Chromeo P503, which preserves the isoelectric point of the ...
Tópico(s): Microfluidic and Bio-sensing Technologies
2010 - Wiley | Electrophoresis
Pamela N. Nge, Jayson V. Pagaduan, Ming Yu, Adam T. Woolley,
... subsequently labeled with both Alexa Fluor 488 and Chromeo P503, and unreacted dye was rinsed off the ...
Tópico(s): Microfluidic and Bio-sensing Technologies
2012 - Elsevier BV | Journal of Chromatography A
Matthew Geiger, Nicholas W. Frost, Michael T. Bowser,
... this as a source of band broadening. A Chromeo P503 labeled tryptic digest of BSA was used ...
Tópico(s): Protein purification and stability
2014 - American Chemical Society | Analytical Chemistry
James Hyde, Nebojsa Kezunovic, Francisco J. Urbano, Edgar García‐Rill,
... coinjections of a new long-stokes-shift dye, Chromeo 494 (CHR). Fluorescent calcium transients were blocked with ...
Tópico(s): Photoreceptor and optogenetics research
2013 - American Physiological Society | Journal of Applied Physiology
María del Mar Barrios-Romero, Agustín G. Crevillén, José Carlos Díez‐Masa,
... achieved for proteins derivatized with the fluorogenic reagent Chromeo P540. Finally, the method was applied to the ...
Tópico(s): Biosensors and Analytical Detection
2013 - Wiley | Journal of Separation Science
Matthew Geiger, Rachel K. Harstad, Michael T. Bowser,
... surface interactions was assessed using μFFE separations of Chromeo P503 labeled myoglobin and cytochrome c. Myoglobin and ...
Tópico(s): Innovative Microfluidic and Catalytic Techniques Innovation
2015 - American Chemical Society | Analytical Chemistry
Marjukka Elmeranta, Giuseppe Vicidomini, Martí Duocastella, Alberto Diaspro, Gustavo de Miguel,
... containing isopropyl thioxanthone (ITX) as the photoinitiator and Chromeo 488 carboxylic acid derivative as a fluorescent reporter, ...
Tópico(s): Nanofabrication and Lithography Techniques
2016 - Optica Publishing Group | Optical Materials Express
Manna Iwabuchi, M.M. Hetu, William G. Tong,
... isothiocyanate, QSY 35 acetic acid, succinimidyl ester, and Chromeo P503 were determined to be 1.4 × 10(- ...
Tópico(s): Parkinson's Disease Mechanisms and Treatments
2016 - Elsevier BV | Analytical Biochemistry
Agustín G. Crevillén, Mercedes de Frutos, José Carlos Díez‐Masa,
... off-chip fluorescently labeled with the fluorogenic reagent Chromeo P503. Then, several separation parameters in t-ITP- ...
Tópico(s): Microfluidic and Bio-sensing Technologies
2017 - Elsevier BV | Microchemical Journal
Ibolya Rutkai, Wesley R. Evans, Nikita Bess, Tomas Salter‐Cid, Siniša Čikić, Partha K. Chandra, Prasad V. G. Katakam, Ricardo Mostany, David W. Busija,
... addition, we observed an overlapping mito-Dendra2 and Chromeo mitochondrial staining of cultured brain microvascular endothelial cells. ...
Tópico(s): Neuroinflammation and Neurodegeneration Mechanisms
2020 - American Physical Society | AJP Heart and Circulatory Physiology
Zichuan Zhang, Jeehae Park, Hannah Barrett, Scott Dooley, Claire L. Davies, Marc F. J. M. Verhagen,
... for AAV capsid proteins using the pyrylium dye Chromeo™ P503, enabling the establishment of the first capillary ...
Tópico(s): RNA Interference and Gene Delivery
2020 - Mary Ann Liebert, Inc. | Human Gene Therapy
Raúl Garrido‐Medina, José Carlos Díez‐Masa, Mercedes de Frutos,
... IA, as covalent tags of thiol groups; and Chromeo™ P503, as a covalent tag of amino groups, ...
Tópico(s): Microfluidic and Capillary Electrophoresis Applications
2013 - Wiley | Electrophoresis
Elliot J. Rapp, Suresh Maximin, Douglas E. Green,
... falls short. Salon.com. http://www.salon.com/chromeo/article/ditch_the_10000_hour_rule_why_malcolm_ ...
Tópico(s): Clinical Reasoning and Diagnostic Skills
2014 - Radiological Society of North America | Radiographics
Cecilia C. Douma, Michael T. Bowser,
... increasing transit distance. Moderate adsorption was observed for Chromeo P503-labeled myoglobin and cytochrome c with temporal ...
Tópico(s): Innovative Microfluidic and Catalytic Techniques Innovation
2023 - American Chemical Society | Analytical Chemistry
Mohamed K. Al‐Essa, Ebaa Alzayadneh, Kamal Al-Hadidi,
... after 30-60 minutes incubation with trypsin by Chromeo P503 (Py 1 pyrylium compound) and CBQCA (3-( ... versus controls (which reflects proteolysis) when assayed by Chromeo P503 (Py 1 pyrylium compound) and CBQCA (3-( ...
Tópico(s): Ubiquitin and proteasome pathways
2021 - Bentham Science Publishers | Protein and Peptide Letters
... on a post‐column sheath flow cuvette employing Chromeo P503 as a fluorogenic reagent for protein labeling ...
2009 - Wiley | Electrophoresis
... Neuroscience Institute in Goettingen.Nuclear structures visualized with Chromeo 494 (green) a d At o 647N red ...
Tópico(s): Cell Image Analysis Techniques
2011 - Future Science Ltd | BioTechniques
Ibolya Rutkai, Wesley R. Evans, Nikita Bess, Tomas Salter‐Cid, Siniša Čikić, Prasad V. G. Katakam, Ricardo Mostany, David W. Busija,
... addition, we observed an overlapping mitoDendra2 FP and Chromeo mitochondrial staining of cultured brain microvascular endothelial cells. ...
Tópico(s): Neurological Disease Mechanisms and Treatments
2019 - Wiley | The FASEB Journal
María del Mar Barrios-Romero, Agustín G. Crevillén, Ángel Puerta, Mercedes de Frutos, José Carlos Díez‐Masa,
... forms of α1-acid glycoprotein (AGP) labeled with Chromeo P540, using SU-8 microchips and laser induced ...
Tópico(s): Microfluidic and Bio-sensing Technologies
2019 - Springer Science+Business Media | Methods in molecular biology
... SARS-CoV-2 nucleocapsid protein labeled with the Chromeo P540 tag using a 532 nm solid-state ...
Tópico(s): Advanced Biosensing Techniques and Applications
2024 - Institute of Physics | Meeting abstracts/Meeting abstracts (Electrochemical Society. CD-ROM)
James Suprapto, William G. Tong,
... labeled with fluorophore and chromophore labels. We utilized Chromeo P540 dye to label the peptides for wave- ...
Tópico(s): Spectroscopy Techniques in Biomedical and Chemical Research
2022 - RELX Group (Netherlands) | SSRN Electronic Journal