Rana Jahanban-Esfahlan, Khaled Seidi, Hassan Monhemi, Amir Daei Farshchi Adli, Babak Minofar, Payman Zare, Davoud Farajzadeh, Safar Farajnia, Ramezan Behzadi, Mehran Mesgari Abbasi, Nosratollah Zarghami, Tahereh Javaheri,
... novel bi-functional fusion protein consisting of truncated coagulase (tCoa) bearing an RGD motif on its C-terminus for cancer therapy. We demonstrated that free coagulase failed to elicit any significant thrombotic activity. Conversely, RGD delivery of coagulase retained coagulase activity and afforded favorable interaction of fusion proteins ... in vitro, and in vivo experiments. Although free coagulase elicited robust coagulase activity in vitro, only targeted coagulase (tCoa-RGD) was capable of producing extensive thrombosis, ...
Tópico(s): Platelet Disorders and Treatments
2017 - Nature Portfolio | Scientific Reports
... Vignaroli C, Varaldo PE (2000) Glycopeptide resistance in coagulase-negative staphylococci. Eur J Clin Microbiol Infect Dis ... diagnosis of catheter-related bloodstream infection caused by coagulase-negative staphylococci. J Med Microbiol Feb 56(Pt ... MA (1996) The positive predictive value of isolating coagulase-negative staphylococci from blood cultures. Clin Infect Dis ... and clinical significance of blood cultures positive for coagulase-negative staphylococcus. Infect Control 6(12): 479 Crossref, ... Liu Y, Luo EJ (2011) Antibiotic susceptibility of coagulase-negative staphylococci (CoNS): emergence of teicoplanin-non-susceptible ...
Tópico(s): Bacterial Identification and Susceptibility Testing
2015 - MA Healthcare | British Journal of Hospital Medicine
Stephen Donoghue, Dunja Ana Vekic, Michael C. Wehrhahn, Margot Whitfeld,
... lugdunensis has been reported as a highly virulent coagulase-negative staphylococcus (CoNS) species implicated in endocarditis and ... variable in a pure culture and resemble mixed coagulase negative staphylococci or mixed skin flora. Furthermore, while belonging to the coagulase-negative staphylococci family, a variable proportion of strains are positive for clumping factor (bound coagulase) and are sometimes detected on slide-based tests used for the detection of coagulase. This may lead to the misidentification of S. ...
Tópico(s): Mycobacterium research and diagnosis
2014 - Wiley | Australasian Journal of Dermatology
... the 21 instances, concordant microbial growth of predominantly coagulase-negative staphylococci from percutaneously drawn blood cultures developed ... al.Endoluminal colonization as a risk factor for coagulase-negative staphylococcal catheter-related bloodstream infections in haemodialysis ... surveillance cultures for prediction of septicaemia caused by coagulase-negative staphylococci in patients undergoing haemodialysis with central ... NR/every 7d/16% after catheter insertion for coagulase-negative staphylococci/NRNR/when suspected/NR/NR/NRFux ...
Tópico(s): Bacterial biofilms and quorum sensing
2014 - Elsevier BV | Kidney International
Oleksa Rewa, John Muscedere, Steve Reynolds, Xuran Jiang, Daren K. Heyland,
To examine the relationship between the isolation of coagulase-negative Staphylococcus in blood cultures and acute phase ... the remaining 292 patients, 31 (10.7%) grew coagulase-negative Staphylococcus, 59 (20.2%) grew known pathogenic ... CRP 164 mg/L), were similar between the coagulase-negative Staphylococcus and negative culture groups (IL-6 ... and CRP 41.0 mg/L). In the coagulase-negative Staphylococcus group, patients who died by day ... survivors, although the differences were not statistically significant.Coagulase-negative Staphylococcus isolated from blood cultures were associated ...
Tópico(s): Neonatal and Maternal Infections
2012 - Pulsus Group | Canadian Journal of Infectious Diseases and Medical Microbiology
Christine L. Cain, Daniel O. Morris, Kathleen O’Shea, Shelley C. Rankin,
... as S schleiferi . Isolates underwent slide and tube coagulase testing and antimicrobial susceptibility testing. A mecA PCR ... ears, and other sites, respectively; 110 (68%) were coagulase negative, and 51 (32%) were coagulase positive. Among the coagulase-negative and coagulase-positive isolates, 65% (71/110) and 39% (20/ ... representatives of both subspecies. Conclusions and Clinical Relevance —Coagulase-positive and coagulase-negative isolates were not genotypically distinct and may represent a single S schleiferi sp with variable coagulase production, rather than 2 biologically distinct subspecies. Further ...
Tópico(s): Antifungal resistance and susceptibility
2011 - American Veterinary Medical Association | American Journal of Veterinary Research
David Patrick Kateete, Cyrus N Kimani, Fred Ashaba Katabazi, Alfred Okeng, Moses Okee, Ann Nanteza, Moses Joloba, Florence Najjuka,
... limited settings. In many developing countries, the tube coagulase test is usually confirmatory for S. aureus and ... test for improving the efficiency of the tube coagulase test in resource limited settings. The efficiency of human and sheep plasma with tube coagulase tests was also evaluated. Methods One hundred and ... growth on Mannitol salt agar, deoxyribonuclease and tube coagulase tests. Of these, isolates that were positive for ... used to evaluate the performance of the tube coagulase test for identification of S. aureus , using PCR- ...
Tópico(s): Bacterial Identification and Susceptibility Testing
2010 - BioMed Central | Annals of Clinical Microbiology and Antimicrobials
Jaehoon Lee, Kwang‐Young Song, Ji‐Yeon Hyeon, In‐Gyun Hwang, Hyo‐Sun Kwak, Jeong‐A Han, Yun-Hee Chung, Kun‐Ho Seo,
... 자체 개발한 real-time PCR법의 검출도를 비교하였고 황색포도상구균 coagulase 확인시험과 real-time PCR법을 활용한 colony PCR 확인 ... 검출능력을 비교하였다. 배지법에서 획득한 의심 집락의 확인 동정은 coagulase 시험과 realtime PCR법을 활용한 colony PCR 확인시험을 병행하여 ... 가공식품인 우유나 소시지 등에서 황색포도상구균을 검출할 경우에는 배지법의 coagulase 확인시험과 colony PCR 확인시험과의 유의차가 없었으며 colony PCR ... 상대적으로 상재균수가 많은 비가공식품인 생 돼지고기나 야채 샘플에서는 coagulase 시험법을 사용한 황색포도상구균의 확인동정은 유효성이 매우 떨어졌으며 colony ... Real-time PCR법을 사용한 24시간 신속 검출법은 배지법의 coagulase 시험법의 양성 수보다 낮은 검출률을 보였으나 colony PCR ... 대체하여 24시간 이내에 신속하게 황색포도상구균을 검출할 수 있고 coagulase 확인 시험을 대체할 수 있는 방법으로 여겨진다. Staphylococcus ...
Tópico(s): Probiotics and Fermented Foods
2010 - Korean Society for Food Science of Animal Resources | Korean Journal for Food Science of Animal Resources
Produção Nacional
Revisado por pares
Jamaira Fereira Veras, Luiz Simeão do Carmo, Lawrence Tong, Jeffrey W. Shupp, Christiano Cummings, Deise Aparecida dos Santos, M.M.O.P. Cerqueira, Alvaro Cantini, Jacques R. Nicoli, Marti Jett,
... was to identify enterotoxin genes from isolates of coagulase-negative staphylococci and coagulase-positive staphylococci obtained from dairy products, responsible for ... From the pool of 152 staphylococcal isolates, 15 coagulase-negative and 15 coagulase-positive representatives were selected for this study. The 15 coagulase-negative isolates were tested for the presence of ... presence of toxin by immunoassay.Seven of the coagulase-negative isolates amplified the coa gene and were subsequently reclassified as coagulase-positive. Twenty-one of 30 selected isolates had ...
Tópico(s): Enterobacteriaceae and Cronobacter Research
2008 - Elsevier BV | International Journal of Infectious Diseases
Tobias Strunk, Peter Richmond, Karen Simmer, Andrew Currie, Ofer Levy, David Burgner,
Purpose of review Coagulase-negative staphylococci have emerged as the most common nosocomial pathogen in neonatal intensive care units worldwide. Our understanding of the interactions between coagulase-negative staphylococci and the immune system is incomplete, ... current knowledge on the human immune response to coagulase-negative staphylococci, with particular emphasis on the neonatal ... very limited data on innate immune responses to coagulase-negative staphylococci in neonates. Levels of serum proteins, ...
Tópico(s): Neutrophil, Myeloperoxidase and Oxidative Mechanisms
2007 - Lippincott Williams & Wilkins | Current Opinion in Infectious Diseases
Christopher N. Ta, Lingmin He, Eddy V. Nguyen, Herminia Miño de Kaspar,
... results in the selection of fluoroquinolone-resistant conjunctival coagulase-negative Staphylococcus. Design. Prospective randomized trial. Methods Patients ... 70 patients (69%) in the study group harbored coagulase-negative Staphylococcus. Among these coagulase-negative Staphylococcus, 12 out of 53 in the ... ofloxacin (p>0.9999). At T1, significantly fewer coagulase-negative Staphylococcus (p=0.0003) were isolated from the study group (18 coagulase-negative Staphylococcus), compared the control group (48 coagulase- ...
Tópico(s): Ocular Diseases and Behçet’s Syndrome
2006 - SAGE Publishing | European Journal of Ophthalmology
... S. Epidemiology of CAPD-associated peritonitis caused by coagulase-negative staphylococci: comparison of strains isolated from hands, ... S. Epidemiology of CAPD-associated peritonitis caused by coagulase-negative staphylococci: comparison of strains isolated from hands, ... of episodes in Canada. Among Gram-positive organisms, coagulase-negative Staphylococcus was the most common accounting for ... 0065751.200.0052Culture negative36215.930.0585116118.540.0805CoNS, coagulase negative staph. Open table in a new tab ...
Tópico(s): Health Systems, Economic Evaluations, Quality of Life
2006 - Elsevier BV | Kidney International
Shaukat Iqbal Malik, Haihong Peng, Mary Barton,
... penicillin G, oxacillin, tetracyclines and enrofloxacin and that coagulase-positive staphylococci from cats were much more resistant when compared to coagulase-negative species (Lilenbaum et al. 1998,1999). This ... et al. 1994). An increase in resistance in coagulase-negative staphylococci to cotrimoxazole, lincomycin, enrofloxacin or oxytetracycline ... is apparent therefore that it is not only coagulase-positive species from cats that have developed resistance mechanisms to various antibiotics but coagulase-negative species which constitute the normal bacteria flora ...
Tópico(s): Antimicrobial Peptides and Activities
2005 - Oxford University Press | Journal of Applied Microbiology
... high frequency, significant clinical impact, and cost of coagulase-negative staphylococcal infections, a great deal of effort ... investigation of the epidemiology, prevention, and treatment of coagulase-negative staphylococcal infection in the neonate and infant. ... patients appear to be high-risk groups for coagulase-negative staphylococcal infections. Coagulase-negative staphylococcus appears to be the major pathogen ... phase II-III clinical trials) for prevention of coagulase-negative staphylococcal infections in high-risk neonates. Resistance ...
Tópico(s): Ocular Infections and Treatments
2004 - Lippincott Williams & Wilkins | Current Opinion in Infectious Diseases
... a case of vertebral osteomyelitis due to the coagulase-negative staphylococcus, Staphylococcus lugdunensis. This is only the ... s lumbar spine inflammatory mass was drained, the coagulase-negative staphylococcus obtained was discarded as a likely contaminant. We discuss the situation when coagulase-negative staphylococci require further identification and look at ... a case of vertebral osteomyelitis due to the coagulase-negative staphylococcus, Staphylococcus lugdunensis. This is only the ... s lumbar spine inflammatory mass was drained, the coagulase-negative staphylococcus obtained was discarded as a likely ...
Tópico(s): Bacterial Identification and Susceptibility Testing
2003 - Elsevier BV | Clinical Microbiology and Infection
Rebecca Jones, Mary Anne Jackson, Catherine Ong, Gary K. Lofland,
... cases from the medical literature. Correctly identifying this coagulase-negative organism is critical because endocarditis is usually ... Urgent surgical intervention is necessary in most cases. Coagulase-negative staphylococci have generally been considered nonpathogenic commensals, ... Freney et al. 2 in 1988, is a coagulase-negative staphylococcus, which can be differentiated from other coagulase-negative species by its ability to produce ornithine ... three blood cultures obtained at our institution yielded coagulase-negative Staphylococcus subsequently identified as S. lugdunensis. The ...
Tópico(s): Streptococcal Infections and Treatments
2002 - Lippincott Williams & Wilkins | The Pediatric Infectious Disease Journal
Anna Marchese, Gian Carlo Schito,
... bacteriostatic activity against many important pathogens including MRSA, coagulase-negative staphylococci and VRE. Bactericidal activity was demonstrated ... 100592 and U-100766 against Staphylococcus aureus and coagulase negative Staphylococcus species.Antimicrob Agents Chemother. 1997; 41: ... 100592 and U-100766 against Staphylococcus aureus and coagulase negative Staphylococcus species.Antimicrob Agents Chemother. 1997; 41: ... against methicillin-resitant Staphylococcus aureus and teicoplanin-intermediate coagulase-negative Staphylococci [abstract 2994].in: Program and Abstracts ...
Tópico(s): Antibiotic Use and Resistance
2001 - Elsevier BV | Clinical Microbiology and Infection
Yasuhiro Okada, Nigel Klein, H. K. F. van Saene, Gary D. Webb, H Holzel, Agostino Pierro,
... TPN) in predisposing infants to infection caused by coagulase-negative staphylococci.Total parenteral nutrition is an important ... been associated with complications, particularly infection caused by coagulase-negative staphylococci. Recent data suggest that TPN may ... function; however, reports directly indicating impaired immunity against coagulase-negative staphylococci during TPN are limited.Study 1 ... used to measure the host bactericidal activity against coagulase-negative staphylococci. Bacterial killing and phagocytosis were measured ...
Tópico(s): Intestinal Malrotation and Obstruction Disorders
2000 - Lippincott Williams & Wilkins | Annals of Surgery
Martin Mempel, Tanja Schmidt, Stephan Weidinger, Christina Schnopp, Johannes Ring, Dietrich Abeck, Timothy J. Foster,
... of staphylococcal protein A (spa), clumping factor (clfA), coagulase (coa), and the fibronectin-binding proteins A and ... of staphylococcal protein A (spa), clumping factor (clfA), coagulase (coa), and the fibronectin-binding proteins A and ... Cambridge University Press, Cambridge1997Google Scholar). Among these are coagulase and clumping factor (Phonimdaeng et al., 1990Phonimdaeng P. ... P. Bramkley A.J. Foster T.J. The coagulase of Staphylococcus aureus 8325–4: sequence analysis and virulence of site-specific coagulase-deficient mutants.Mol Microbiol. 1990; 4: 393-404Crossref ...
Tópico(s): Nail Diseases and Treatments
1998 - Elsevier BV | Journal of Investigative Dermatology
Sherif B. Mossad, Janet Serkey, David L. Longworth, Delos M. Cosgrove, Steven M. Gordon,
Coagulase-negative staphylococci are commonly isolated from wounds of patients after median sternotomy; however, the epidemiology of ... all patients with sternal wound infections attributable to coagulase-negative staphylococci after 22,180 open heart procedures ... risk factors for sternal wound infections caused by coagulase-negative staphylococci, 17 patients with coagulase-negative staphylococcal sternal wound infections were compared with ... procedures), of which 100 (23%) were attributable to coagulase-negative staphylococci (4.5 per 1,000). Fifty- ...
Tópico(s): Orthopedic Infections and Treatments
1997 - Elsevier BV | The Annals of Thoracic Surgery
Benjamin R. Vowels, David S. Feingold, C Sloughfy, Arlene N. Foglia, Nellie Konnikov, Elsa Ordoukhanian, Prentice Starkey, William D. James,
... an increased density of cutaneous erythromycin-resistant (Emr) coagulase-negative staphylococci; however, it is unknown if this ... or if upon cessation of erythromycin use, Emr coagulase-negative staphylococci remain at an increased density compared ... 8 micrograms/ml) were used to identify Emr coagulase-negative staphylococci. The species of all Emr coagulase-negative staphylococci were determined, and an antibiogram for ... antibiotics was obtained. The baseline prevalence of Emr coagulase-negative staphylococci on the forehead and nose was ...
Tópico(s): Bacterial Identification and Susceptibility Testing
1996 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Kirstine Eastick, John P. Leeming, David M. Bennett, Michael Millar,
... the magnitude of, and interrelations between, reservoirs of coagulase negative staphylococci on infants' skin at various sites ( ... months of life. Sites with large numbers of coagulase negative staphylococci were identified by sampling 16 skin ... more detailed survey of numbers and types of coagulase negative staphylococci in stool and at six skin ... the first six months of life. Isolates of coagulase negative staphylococci were collected and characterised by speciation, ... are frequently sited, carried small unstable numbers of coagulase negative staphylococci, which were usually indistinguishable from coagulase ...
Tópico(s): Antimicrobial Resistance in Staphylococcus
1996 - BMJ | Archives of Disease in Childhood Fetal & Neonatal
Jerry R. Roberson, Lawrence K. Fox, Dale D. Hancock, John Gay, Thomas E. Besser,
Abstract Objective To determine prevalence and relevance of coagulase-positive Staphylococcus hyicus and S intermedius intramammary infections ( ... determine the ability of the 4-hour tube coagulase (TC) test to differentiate the coagulase-positive staphylococci (CPS). Design Prevalence of CPS was ... from aseptically collected composite milk samples was performed. Coagulase-positive isolates from 4 cow groups were tested ... 82.1% were S aureus , 17.7% were coagulase-positive S hyicus , and 0.2% were S ... all CPS IMI in LP herds, 34% were coagulase-positive S hyicus ; of all CPS IMI in ...
Tópico(s): Milk Quality and Mastitis in Dairy Cows
1996 - American Veterinary Medical Association | American Journal of Veterinary Research
Marcelo Carvalho Ramos, Michael Ing, E Kim, Mallory D. Witt, Arnold S. Bayer,
... and therapy of endocarditis caused by oxacillin-resistant, coagulase-negative staphylococci in patients with native or prosthetic ... of vancomycin-based oxacillin-resistant, beta-lactamase-producing coagulase-negative staphylococcal isolate (Staphylococcus haemolyticus SE220). Ampicillin-sulbactam ( ... dose regimen) in its prophylactic efficacy against the coagulase-negative staphylococcal strain (93 and 80%, respectively). The ... valve endocarditis in rabbits caused by this same coagulase-negative staphylococcal strain, animals received 7-day ampicillin- ...
Tópico(s): Bacterial Identification and Susceptibility Testing
1996 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Tae Tanaka, Katsuko Okuzumi, Aikichi Iwamoto, Keiichi Hiramatsu,
... of mecA gene, antibiotic susceptibility patterns, toxin production, coagulase isotypes, and ribotyping patterns. The mecA-carrying strains in 1982 were mostly producers of type-4 coagulase (21 out of 31;68%), but producers of other coagulase types (type-1,-2,-7) were also found. ... type in terms of production of type 2 coagulase (26 out of 27;96%), and high-level methicillin resistance (MIC50>-512). Clonal expansion of the coagulase type-2 strain was implicated under selective pressure ... of mecA gene, antibiotic susceptibility patterns, toxin production, coagulase isotypes, and ribotyping patterns. The mecA-carrying strains ...
Tópico(s): Bacterial Identification and Susceptibility Testing
1995 - Elsevier BV | Journal of Infection and Chemotherapy
David G. Armstrong, John Lanthier, Phillip Lelievre, Gary W. Edelson,
Awareness of the virulence of coagulase-negative Staphylococci, previously regarded as saprophytes with minimal pathogenicity, has steadily increased. Eighty-seven individual patients diagnosed with acute osteomyelitis, as ... known to have diabetes mellitus. The prevalence of coagulase negative Staphylococcus was 40% (35/87) in deep ... 35) of which were methicillin resistant. When the coagulase negative Staphylococcus group was assessed for prior long- ... that 54% (12/22) of the methicillin-resistant coagulase-negative Staphylococcal infected patients had received such treatment, ...
Tópico(s): Antimicrobial Resistance in Staphylococcus
1995 - Elsevier BV | The Journal of Foot & Ankle Surgery
Jerry R. Roberson, Lawrence K. Fox, D. D. Hancock, Catherine Gay, T.E. Besser,
Objectives were to determine the prevalence of coagulase-positive staphylococcal IMI in primiparous cows at first parturition, to contrast the differences in coagulase-positive staphylococcal IMI in primiparous cows at parturition in herds with high and low prevalences of coagulase-positive staphylococcal IMI in the lactating herd, and ... determine the percentage of primiparous cows having persistent coagulase-positive staphylococcal IMI. Milk samples were collected aseptically ... categories: high (> 10%) or low (< 5%) prevalence of coagulase-positive staphylococcal IMI. At the start, the mean ...
Tópico(s): Probiotics and Fermented Foods
1994 - Elsevier BV | Journal of Dairy Science
... Fukata, T. & Matsumoto, H. 1980 Zoological studies on Coagulase-negative Staphylococci in and round the udder. Bulletin ... S. & Hirsh, D.C. 1984 Species distribution of coagulase-positive staphylococci in animals. Journal of Clinical Microbiology ... Keyser, H. 1980 Prevalence of different species of coagulase-negative Staphylococci on teats and on milk samples ... K.H. & Adegoke, G.O. 1985 Identification of coagulase-negative Staphylococci from farm animals. Journal of Applied ... J.H. 1957 The distribution of toxins in coagulase-negative Staphylococci isolated from infections and carriers. Journal ...
Tópico(s): Bacterial biofilms and quorum sensing
1990 - Wiley | Journal of Applied Bacteriology
Prasart Phonimdaeng, Mary O’Reilly, Peter Nowlan, A.J. Bramley, Timothy J. Foster,
Summary The sequence of the coagulase gene ( coa ) from Staphylococcus aureus strain 8325‐4 is reported. The deduced amino acid sequence of the coagulase protein is compared with previously reported sequences of coagulases from strains 213 and BB. The secreted mature forms of coagulase proteins are composed of three distinct segments: (i) ... sequences are probably responsible for antigenic differences among coagulases of different serotype. The region of coagulase which binds to prothrombin and activates it to ...
Tópico(s): Biochemical and Structural Characterization
1990 - Wiley | Molecular Microbiology
Angela S. Rossney, L. English, C. T. Keane,
... Comparison was made with two methods of tube coagulase testing and five slide methods for detecting clumping factor (slide coagulase testing). Performances were assessed for two groups of ... and non-staphylococcal species group, the kits and coagulase methods performed as follows (the figures in parentheses ... efficiency values for the staphylococcal group alone): tube coagulase reference method 100% (100%), tube coagulase SJH method 99% (99%), Staphaurex 94% (97%), Staphylase 93% (96%), slide coagulase method No 4 93% (94%), slide coagulase method ...
Tópico(s): Cell Image Analysis Techniques
1990 - BMJ | Journal of Clinical Pathology