Limpar
3.136 resultados

Acesso aberto

Tipo do recurso

Ano de criação

Produção nacional

Revisado por pares

Áreas

Idioma

Editores

Jornais Acesso aberto

James Landale, Giles Tremlett, Raymond Snoddy Media Editor, Pamela Couper, Richard Morrison, Arts Editor, Helen Johnstone, D. G. McGill, Alasdair Murray, Economics Correspondent, and Oliver August, Robert Cole, Fred Redwood, Julian Muscat, Tennis Correspondent, Adrian Lee, Morris of Castle Morris, Sarah Potter, Charles Lewington, Paul Hoggart, Mark Souster, Richard Hobson, Gavin Lumsden, Philip Howard, Hilary Finch, Don Carios, J. N. Lack, Jeremy Kingston, Joanna Bale, James Bone, Rachel Campbell-Johnston, Lucy Hawking, Alasdair Murray, Anne Ashworth and Clare Stewart, Oliver Holt Football Correspondent, Michael Evans, Defence Editor, Jeremy Whittle, Tom Walker, Christine Buckley Industrial Correspondent, Raymond Snoddy, Gerald Davies, Robert Sheehan, Bridge Correspondent, Joe Joseph, Roland Watson Political Correspondent, Ruth Gledhill, Religion Correspondent, Julian Lewis, Christopher Thomas and Michael Theodoulou, Stewart Tendler Crime Correspondent, John Hopkins, Golf Correspondent, Dominic Walsh, Ruth Gledhill, Matthew Barbour, Raymond Keene Chess Correspondent, John Street, Michael Nicholson, Richard Evans, Racing Correspondent, Dalya Alberge Arts Correspondent, Jon Wynne-Tyson, John Ward, Robin Young, Mark Inglefield, Brian Wilson, Jill Sherman Chief Political Correspondent, Chris Ruff, Rodney Milnes, Jenny MacArthur, Michael Clark, Andy Sibson, Philharmonia/Gardiner, John O'Leary, Marianne Curphey, Insurance Correspondent, Audrey Magee, David Hands, Martin Fletcher Chief Ireland Correspondent, Chris Barton, Deborah Collcutt, Victoria Fletcher, George Caulkin and Russell Kempson, Luke Clancy, Jasper Gerard, Valerie Elliott Whitehall Editor, Russell Kempson, Nigel Williamson, Simon Midgley, Meg Carter, Paul Durman, Chris Buckland, Manus Costello, Julia Neuberger, Chief Executive, Andy Lavender, Richard Brown, John Barnes, Alison Roberts, Arnaido Cohen, Maggie Brown's, Arthur Leathley Aviation Correspondent, Raymond Keene, Edward Martin, Christine Buckley, Tony Whelpton, Lewis Smith, Frances Gibb, Legal Correspondent, Stefano Hatfield, Francis Gilbert, Bronwen Maddox, Alan Pearey, Richard Owen, Damian Whitworth, Sidney Friskin, Erica Wagner, Roger Boyes, Sarah Cunningham, SparKlehorse, Oliver August, Ian Murray Medical Correspondent, Philip Webster, Political Editor, David J. Prescott, Hettie Judah, Dalya Alberge, Oliver Holt, Football Correspondent, A. E. C. W. Spencer, Michael Evans, Charles Bremner, and Philip Webster, Valerie Elliott, Whitehall Editor, Richard Duce, John Allison, Christopher Thomas, Giles Whittell, Nick Nuttall, Technology Correspondent, Christopher Walker, Chris Parker, Simon De Bruxelles, Brian MacArthur, Matthew Parris, Graham Lacey, George Caulkin, Michael Hornsby, John Shaw, Edward du Cann, Adam Wright, Caitlin Moran, Walter Gammie, Peter Riddell, Ben MacIntyre, Philip Webster, David Sinclair, Simon Jenkins, Mark Henderson, Martin Waller, Michael Horsnell, Gillian Harris, G. A. Hindes (teacher), Chris Ayres, Carl Mortished International Business Editor, Tim Southwell, Martin Lee, Frances Gibb Legal Correspondent, Richard Morrison, James Christopher, Valerie Grove, Alasdair Murray, Mr Ripley, Patience Wheatcroft, Alasdair Murray, Economics Correspondent, Christopher Irvine,

... power industry Buyer aware Well suited Cult figure COPI Employers taust rethink how to get best from ...

1998 - Gale Group | TDA

Artigo Acesso aberto Revisado por pares

Chisa Shinotsuka, Yusaku Yoshida, Kazumasa Kawamoto, Hiroyuki Takatsu, Kazuhisa Nakayama,

... of small GTPases, which regulate membrane association of COPI and adaptor protein (AP)-1 coat protein complexes. ... AP-1 complex but not that of the COPI complex. These observations indicate that BIG2 is implicated ... association of AP-1, but not that of COPI, through activating ARF. Furthermore, not only BIG2 but ... of small GTPases, which regulate membrane association of COPI and adaptor protein (AP)-1 coat protein complexes. ... AP-1 complex but not that of the COPI complex. These observations indicate that BIG2 is implicated ...

Tópico(s): Calcium signaling and nucleotide metabolism

2002 - Elsevier BV | Journal of Biological Chemistry

Outros Acesso aberto

Viscount Sidmouth,

... March to April. Letter. From Viscount Sidmouth. To Copi Disbrowe. New Street.

0000 - Gale Group | NCCO BritishPolitics

Artigo Acesso aberto Revisado por pares

Elena V. Gazina, Jason M. Mackenzie, Rebecca J. Gorrell, David A. Anderson,

... EV11) replication is strongly inhibited. Since BFA inhibits COPI-dependent steps in endoplasmic reticulum (ER)-Golgi transport, ... that different picornaviruses may have differential requirements for COPI in the formation of their RCs. Using immunofluorescence ... immunoelectron microscopy we examined the association of a COPI component, β-COP, with the RCs of EMCV, ... that there are differences in the involvement of COPI in the formation of the RCs of various ... after vesicle budding from the ER, prior to COPI association with membranes. ParV1 RCs are formed from ...

Tópico(s): CRISPR and Genetic Engineering

2002 - American Society for Microbiology | Journal of Virology

Artigo Acesso aberto Revisado por pares

Emmanuel G. Reynaud, Jeremy C. Simpson,

... and ζ) and the GTPase ARF1, together termed COPI. Although the COPI coat has been seemingly well characterized, C. Reinhard (Heidelberg, Germany) described two new subunits of the COPI complex: γ2 and ζ2. The γ2-COP subunit ... determined that these two subunits exist in the COPI coat either in a γ1ζ2 or in a ... γ2-COP subunit is enriched in Golgi-derived COPI vesicles, whereas the γ1-COP subunit remains associated ... into how cells manage to efficiently utilize the COPI coat to recycle material backwards while still maintaining ...

Tópico(s): Lipid Membrane Structure and Behavior

2002 - Springer Nature | EMBO Reports

Artigo Acesso aberto Revisado por pares

Holger M. Rohde, Fei Ying Cheong, Gerlinde Konrad, Karin Paiha, Peter Mayinger, Guido Boehmelt,

... the first time members of the coatomer I (COPI) complex as interaction partners of hSAC1. Mutation of a putative COPI interaction motif (KXKXX) at its C terminus abolishes interaction with COPI and causes accumulation of hSAC1 in the Golgi. ... in the Golgi, incapable of interacting with the COPI complex despite the presence of the KXKXX motif. ... hSAC1 provides a switch for accessibility of the COPI interaction motif. The Saccharomyces cerevisiae SAC1 gene encodes ... the first time members of the coatomer I (COPI) complex as interaction partners of hSAC1. Mutation of ...

Tópico(s): Ubiquitin and proteasome pathways

2003 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

Mickaël M. Cohen, Françoise Stutz, Catherine Dargemont,

... the endoplasmic reticulum. Further analysis indicate that the COPI subunit β′-COP represents another substrate of the ... the endoplasmic reticulum. Further analysis indicate that the COPI subunit β′-COP represents another substrate of the ... 20: 3869-3879Crossref PubMed Scopus (140) Google Scholar). COPI bovine subunits fused to the Gal4 activation domain ... ζ-COP) form the coat protein complex I (COPI) involved in retrograde transport within the Golgi apparatus ( ... been clearly shown that dilysine signals confer a COPI-mediated localization to the ER (13Letourneur F. Gaynor ...

Tópico(s): Ubiquitin and proteasome pathways

2003 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

Constanze Reinhard, Michael Schweikert, Felix Wieland, Walter Nickel,

Coat protein I (COPI)-coated transport vesicles mediate protein and lipid transport in the early secretory pathway. The basic machinery required for the ... has been elucidated based on the reconstitution of COPI-coated vesicle formation from chemically defined liposomes. In ... as p23 as a membrane-bound receptor for COPI coat proteins, were shown to be both necessary and sufficient to promote COPI-coated vesicle formation. Based on biochemical and ultrastructural ... is sufficient to initiate uncoating of liposome-derived COPI-coated vesicles. By contrast, ARF-GAP activity is ...

Tópico(s): RNA Interference and Gene Delivery

2003 - National Academy of Sciences | Proceedings of the National Academy of Sciences

Artigo Acesso aberto Revisado por pares

Jonathon L. Burman, Lyne Bourbonnière, Jacynthe Philie, Thomas Stroh, Selma Y. Dejgaard, John F. Presley, Peter S. McPherson,

... binding partners and identified components of coatomer I (COPI) coats. The interaction was confirmed in pull-down ... for a non-transmembrane domain protein, Scyl1 binds COPI coats using a C-terminal RKLD-COO(-) sequence, similar to the KKXX-COO(-) COPI-binding motif found in transmembrane endoplasmic reticulum (ER) ... intermediate compartment and the cis-Golgi, sites of COPI-mediated membrane budding. The localization and binding properties of Scyl1 strongly suggest a function in COPI transport, and inhibitory RNA-mediated knock down of ...

Tópico(s): Genetic Neurodegenerative Diseases

2008 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

Jia-Shu Yang, Helge Gad, Stella Y. Lee, Mironov Aa, Leiliang Zhang, Galina V. Beznoussenko, Carmen Valente, Gabriele Turacchio, Akua N. Bonsra, Guangwei Du, Gianluca Baldanzi, Andrea Graziani, Sylvain Bourgoin, Michael A. Frohman, Alberto Luini, Victor W. Hsu,

Two distinct steps drive COPI vesicle fission: bud-neck constriction, which is dependent on the protein BARS and COPI constituents, followed by bud-neck scission, which is ... for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known ... substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces ... This finding has allowed us to further delineate COPI vesicle fission into two sub-stages: 1) an ... bud-neck constriction, in which BARS and other COPI components are required, and 2) a later stage ...

Tópico(s): Calcium signaling and nucleotide metabolism

2008 - Nature Portfolio | Nature Cell Biology

Artigo Acesso aberto Revisado por pares

C. Randell Brown, Allison B. Wolfe, Dongying Cui, Hui-Ling Chiang,

... degradation. SEC28 encodes the ϵ-COP subunit of COPI (coat protein complex I) coatomer proteins. When SEC28 ... degradation. SEC28 encodes the ϵ-COP subunit of COPI (coat protein complex I) coatomer proteins. When SEC28 ... thoroughly studied transporters are coat protein complex I (COPI), 2The abbreviations used are: COPIcoat protein complex IERendoplasmic ... import and degradation. COPII, and clathrin-coated vesicles. COPI vesicles mediate the retrograde transport from the Golgi ... desorption ionization vacuole import and degradation. Characterization of COPI vesicles has revealed the presence of a protein ...

Tópico(s): Autophagy in Disease and Therapy

2008 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

Yoichi Noda, Koji Yoda,

... acquiring those of the later compartments in a COPI vesicle-dependent manner (2Losev E. Reinke C.A. ... certain maturation stage is carried out by the COPI vesicles after activation of the G-protein Arf1 ... exit and entry of selected components in the COPI vesicles. Therefore, the selective incorporation of the cargo proteins in the COPII and COPI vesicles should be very important for maintaining the ... 398) Google Scholar) or K(X)KXX for COPI selection (11Cosson P. Letourneur F. Science. 1994; 263: ... acquiring those of the later compartments in a COPI vesicle-dependent manner (2Losev E. Reinke C.A. ...

Tópico(s): Photosynthetic Processes and Mechanisms

2010 - Elsevier BV | Journal of Biological Chemistry

Carta Acesso aberto Revisado por pares

Frederick M. Hughson,

The assembly of COPI into a cage-like lattice sculpts membrane vesicles that transport cargo from the Golgi apparatus. Now, Lee and Goldberg, ... Google Scholar present X-ray crystal structures of COPI suggesting that these coats combine selected features of ... archetypal coats, clathrin and COPII. The assembly of COPI into a cage-like lattice sculpts membrane vesicles ... Google Scholar present X-ray crystal structures of COPI suggesting that these coats combine selected features of ... endoplasmic reticulum (ER). The third archetypal coat is COPI. ARF family G proteins recruit COPI to the ...

Tópico(s): Cellular transport and secretion

2010 - Cell Press | Cell

Artigo Acesso aberto Revisado por pares

Danming Tang, Kari Mar, Graham Warren, Yanzhuang Wang,

... of this protein. Vesiculation is mediated by the COPI budding machinery ARF1 and the coatomer complex. When ... of this protein. Vesiculation is mediated by the COPI budding machinery ARF1 and the coatomer complex. When ... mitotic kinases, and membrane vesiculation mediated by the COPI vesicle budding machinery. Post-mitotic Golgi reassembly also ... vertical rotor (NVT65, Beckman Instruments, 50,000 rpm). COPI-coated vesicles typically peaked at 1.2–1. ... vesicles. Six categories of proteins were analyzed: 1) COPI coat proteins ARF1 and β-COP; 2) Golgi ...

Tópico(s): Retinal Development and Disorders

2007 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

Jessica Rennolds, Cristy Tower-Gilchrist, Lois C. Musgrove, Lijuan Fan, Kevin M. Maloney, John P. Clancy, Kevin L. Kirk, Elizabeth Sztul, Estelle Cormet‐Boyaka,

... PM) is important. The coat protein complex I (COPI) has been implicated in the anterograde and retrograde ... the Golgi. Here, we investigated the role of COPI in CFTR trafficking. Blocking COPI recruitment to membranes by expressing an inactive form ... inhibits CFTR trafficking to the PM. Similarly, inhibiting COPI dissociation from membranes by expressing a constitutively active ... Golgi elements. To definitively explore the relationship between COPI and CFTR in epithelial cells, we depleted beta- ... activated chloride currents. To explore the mechanism of COPI action in CFTR traffic we tested whether CFTR ...

Tópico(s): Bacterial Genetics and Biotechnology

2007 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

Yi Xiang, Joachim Seemann, Blaine Bisel, Sukanya Punthambaker, Yanzhuang Wang,

... is a small GTPase that is required for COPI vesicle formation from the Golgi membranes. Treatment of ... the inactive mutant, converted the Golgi membranes into COPI vesicles. ARF1-depleted mitotic cytosol failed to fragment ... is a small GTPase that is required for COPI vesicle formation from the Golgi membranes. Treatment of ... the inactive mutant, converted the Golgi membranes into COPI vesicles. ARF1-depleted mitotic cytosol failed to fragment ... and recruits coatomer onto the membrane to form COPI vesicles. Inactivation of ARF1 in turn prevents recruitment ...

Tópico(s): Lipid Membrane Structure and Behavior

2007 - Elsevier BV | Journal of Biological Chemistry

Revisão Acesso aberto Revisado por pares

Jesse Hay,

... are the vesicle coat–protein complexes, such as COPI and COPII, which select cargo and catalyse the ... modulate processes, for example, coat-protein complex I (COPI)/COPII vesicle coating/uncoating. (D) Calcium-independent. Calcium ... permeant BAPTA-AM caused an apparent redistribution of COPI from Golgi membranes to the cytosol (Ahluwalia et al, 2001). Inspection of in vitro COPI-vesicle budding and binding assays revealed that BAPTA destabilized COPI binding to membranes, and could block vesicle budding ...

Tópico(s): Calcium signaling and nucleotide metabolism

2007 - Springer Nature | EMBO Reports

Artigo Acesso aberto Revisado por pares

Tae Hwa Jeon, Wonyong Choi, Hyunwoong Park,

... semiconductor electrodes were coupled with cobalt–phosphate complexes (CoPi) to enhance the photoelectrochemical (PEC) performance for water oxidation reaction. CoPi was deposited on a 550 nm-thick BiVO4 ... PD) methods for comparison of their effects. The CoPi on BiVO4 exhibited Co : P atomic ratios of ... and Co3+ co-existed in both samples. Optimized CoPi ED resulted in a CoPi overlayer of approximately 850 nm thick, which showed ... limited access of phosphate into BiVO4 across the CoPi layer. Optimized CoPi PD, however, had very thin ...

Tópico(s): Copper-based nanomaterials and applications

2011 - Royal Society of Chemistry | Physical Chemistry Chemical Physics

Artigo Acesso aberto Revisado por pares

Daishi Kitazawa, Masamitsu Yamaguchi, Hajime Mori, Yoshihiro Inoue,

The coatomer protein complex, COPI, mediates retrograde vesicle transport from the Golgi apparatus to the ER. Here, we investigated the meiotic phenotype of Drosophila melanogaster ... genes encoding membrane-trafficking-related factors. We identified COPI as an essential factor for male meiosis. In Drosophila male meiotic divisions, COPI is localized in the ER-Golgi intermediate compartment ... microtubules. At the end of each meiotic division, COPI-containing vesicles are equally partitioned between two daughter ...

Tópico(s): Microtubule and mitosis dynamics

2012 - The Company of Biologists | Journal of Cell Science

Artigo Acesso aberto Revisado por pares

Jia-Shu Yang, Stella Y. Lee, Stefania Spanò, Helge Gad, Leiliang Zhang, Zhongzhen Nie, Matteo Bonazzi, Daniela Corda, Alberto Luini, Victor W. Hsu,

... role for BARS at the fission step of COPI vesicle formation from Golgi membrane Jia-Shu Yang ... Info The core complex of Coat Protein I (COPI), known as coatomer, is sufficient to induce coated ... roles may be linked remains unknown. Moreover, whether COPI vesicle formation from Golgi membrane requires additional factors ... a critical role in the fission step of COPI vesicle formation from Golgi membrane. This role of ... not only identify a new factor needed for COPI vesicle formation from Golgi membrane but also reveal ...

Tópico(s): Erythrocyte Function and Pathophysiology

2005 - Springer Nature | The EMBO Journal

Artigo Acesso aberto Revisado por pares

David Sheff, Martin Lowe, Thomas E. Kreis, Ira Mellman,

The coat protomer complex I (COPI) family of coat proteins are involved in the assembly of membrane-associated coats thought to mediate vesicular transport between the endoplasmic ... investigated whether this heterogeneity in the sites of COPI action might be reflected in biochemical heterogeneity of one or more COPI subunits. A simplified method was devised to purify the cytosolic COPI precursor complex, coatomer, from rat liver cytosol. The ... but no other coatomer subunits, were serine-phosphorylated. COPI phosphorylation may regulate coatomer assembly, membrane recruitment, or ...

Tópico(s): Chemical Synthesis and Analysis

1996 - Elsevier BV | Journal of Biological Chemistry

Revisão Acesso aberto Revisado por pares

Hugh R.B. Pelham, James E. Rothman,

... J.E Bidirectional transport by distinct populations of COPI-coated vesicles.Cell. 1997; 90: 335-349Abstract Full ... PubMed Scopus (337) Google Scholar). They are named COPI vesicles because their budding is driven by the assembly of the COPI coat from cytoplasmically derived subunits (coatomers). The coatomers ... GTP-bound form) and then polymerize into a COPI coat, forming an approximately spherical template that shapes ... a sequential mode of action for COPII and COPI.Cell. 1997; 90: 1137-1148Abstract Full Text Full ...

Tópico(s): Pancreatic function and diabetes

2000 - Cell Press | Cell

Artigo Acesso aberto Revisado por pares

Marie Gomez, Suzie J. Scales, Thomas E. Kreis, Franck Perez,

... temperature-sensitive mutation in the Coat protein I (COPI) subunit ε-COP (Guo, Q., Vasile, E., and ... the nonpermissive temperature, neither the stability of the COPI complex nor its recruitment to donor Golgi membranes ... signal binding is not the major means of COPI recruitment. Because expression of the exogenous chimera of ... role in the cargo selection events mediated by COPI. It has previously been shown that transport of ... temperature-sensitive mutation in the Coat protein I (COPI) subunit ε-COP (Guo, Q., Vasile, E., and ...

Tópico(s): Endoplasmic Reticulum Stress and Disease

2000 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

David J. Hackam, Roberto J. Botelho, Carola Sjölin, Ori D. Rotstein, John M. Robinson, Alan D. Schreiber, Sergio Grinstein,

... processes mediating their release and insertion are unknown. COPI plays an essential role in the budding and ... vesicles in intracellular compartments. We therefore assessed whether COPI is also involved in phagosome formation. We used ... type IIA (FcγRIIA). In the presence of functional COPI, FcγRIIA-transfected ldlF cells effectively internalized opsonized particles. ... the restrictive temperature. Similar results were obtained impairing COPI function in macrophages using brefeldin A. Notably, loss of COPI function preceded complete inhibition of phagocytosis, suggesting that ...

Tópico(s): Monoclonal and Polyclonal Antibodies Research

2001 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

Manfred Boehm,

... or activation of other coat proteins such as COPI (Donaldson et al., 1992; Palmer et al., 1993; ... from the membrane, as has been shown for COPI (Aoe et al., 1997) and AP-3 (Ooi ... between yeast ARF1 and various subunits of the COPI complex could be increased by removal of the ... assays (Paris et al., 1997); it interacted with COPI in vitro and competed with full-length myristoylated ARF1 for COPI recruitment to membranes (Goldberg, 1999). Indeed, we found ... behaves as an inactive GDP-bound protein in COPI–ARF1 two-hybrid interaction studies (Eugster et al., ...

Tópico(s): Lipid Membrane Structure and Behavior

2001 - Springer Nature | The EMBO Journal

Artigo Acesso aberto Revisado por pares

William J. Belden, Charles Barlowe,

... the Emp24p and Erv25p tail sequences with the COPI and COPII coat proteins, binding experiments with immobilized ... of aromatic residues found in both tail sequences. COPI subunits also bound to these Emp24p and Erv25p ... tail sequence, which contains a dilysine motif, bound COPI more efficiently. These results suggest that both the ... from the ER. The Erv25p tail sequence binds COPI and is responsible for returning this complex to ... the Emp24p and Erv25p tail sequences with the COPI and COPII coat proteins, binding experiments with immobilized ...

Tópico(s): Genetics and Neurodevelopmental Disorders

2001 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

Effrosyni Papanikou, Kasey J. Day, Jotham R. Austin, Benjamin S. Glick,

COPI coated vesicles carry material between Golgi compartments, but the role of COPI in the secretory pathway has been ambiguous. Previous studies of thermosensitive yeast COPI mutants yielded the surprising conclusion that COPI was dispensable both for the secretion of certain ... method, which allows peripheral membrane proteins such as COPI to be sequestered rapidly by adding rapamycin. Video fluorescence microscopy revealed that COPI inactivation causes an early Golgi protein to remain ... Golgi proteins, explaining how secretion can persist when COPI has been inactivated. Our findings suggest that cisternal ...

Tópico(s): Endoplasmic Reticulum Stress and Disease

2015 - eLife Sciences Publications Ltd | eLife

Artigo Acesso aberto Revisado por pares

Saskia Schröter, Sabrina Beckmann, Hans Dieter Schmitt,

... 2016free access Transparent process ER arrival sites for COPI vesicles localize to hotspots of membrane trafficking Saskia ... favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Abstract COPI-coated vesicles mediate retrograde membrane traffic from the ... eukaryotic cells. However, it is still unknown whether COPI vesicles fuse everywhere or at specific sites with ... ER arrival sites (ERAS) by monitoring contact between COPI coat components and the ER-resident Dsl tethering ...

Tópico(s): Endoplasmic Reticulum Stress and Disease

2016 - Springer Nature | The EMBO Journal

Artigo Acesso aberto Revisado por pares

Midori Ishii, Yasuyuki Suda, Kazuo Kurokawa, Akihiko Nakano,

... this retrograde transport are coat protein complex I (COPI)-coated vesicles. We examined the COPI function in cisternal maturation directly by 4D observation ... transmembrane Golgi-resident proteins in living yeast cells. COPI temperature-sensitive mutants and induced degradation of COPI proteins were used to knockdown COPI function. For both methods, inactivation of COPI subunits Ret1 and Sec21 markedly impaired the transition ... cisternae within the cytoplasm was severely restricted when COPI subunits were depleted. Our results demonstrate the essential ...

Tópico(s): Fungal and yeast genetics research

2016 - The Company of Biologists | Journal of Cell Science

Artigo Acesso aberto Revisado por pares

Yimeng Ma, Andreas Kafizas, Stephanie R. Pendlebury, Florian Le Formal, James R. Durrant,

... BiVO 4 ), both with and without cobalt phosphate (CoPi) co‐catalyst. These results are correlated with spectroelectrochemical ... Co II oxidation to Co III in a CoPi/FTO (fluorine doped tin oxide) electrode during dark ... results are compared quantitatively with the degree of CoPi oxidation observed under conditions of photoinduced water oxidation on CoPi–BiVO 4 photoanodes. For the CoPi–BiVO 4 photoanodes studied herein, ≤5% of water oxidation proceeds from CoPi sites, making the BiVO 4 surface the predominant ...

Tópico(s): Electrocatalysts for Energy Conversion

2016 - Wiley | Advanced Functional Materials