Bermúdez de la Torre y Solier, Pedro José,
In: Coleccion de folletos. Tom. XXXIII, Literatura y variedades. Cuarta parte, 1630 á 1744. M.O. Lima, 1848 (Latin American pamphlets from the Yale University ...
0000 - Gale Group | Sabin
Yongpeng Guo, Yanan Wang, Yanrong Liu, Qiugang Ma, Cheng Ji, Lihong Zhao,
... present study showed that the induction of spore CotA laccase production of Bacillus licheniformis by ions Mn2+ ... ZEN-degrading ability of the strain. The spore CotA laccase heterologously expressed in Escherichia coli was capable ... as redox mediators could enhance ZEN degradation by CotA laccase. The human breast cancer MCF-7 cell proliferation assay showed that CotA laccase and CotA laccase-mediator systems eliminated the estrogenicity of ZEN. Immobilized CotA laccase onto chitosan microspheres exhibited higher thermal stability ...
Tópico(s): Plant tissue culture and regeneration
2022 - Elsevier BV | LWT
Jonathan Northcroft, Carlos Alba, John Dugdale, Hala Jaber, Nicolas Balfour, Led Zeppelin, Matthew Wall, Barbara Hall, Joan Bakewell, Dan O'Neill, Nigel Botherway, John Smith, Cerys Atkins, June Garfield, E H Davies, Tracey Cox, Heten Vandevelde, N J, Jim Munro, Peter Whittle, Paula Radcliffe, Helen Davies, Sally Brock, Nicholas Rufford, Rita Konig, Rob Hughes, Peter Colons, David Moseley, Indigo Thomas, John Peter, Mark Hodgkinson, Olga Lockley, Professor Gideon Garter, Emma Moore, Amanda Ursell, Abigail Falkner, Andrew Longmore, Lesley White, Jane Mulkerrins, Christopher Somerville, J D, Susan d'Arcy, Gail Robinson, Gareth Walsh, Frank Whitford, Dawna Walter, Ferdinand Mount, Kathryn Cooper, John Elliot, Lucy Hughes-Hallett, David Smith, Pamela Anderson, Tim Richards, Maynard Gilchrist, Peter Sharkey, Rory Godson, J M Coetzee, John Curtis, Neil Wormald, E P, Andrew Sullivan, Clive Davis, Lynne Truss, C D, Jeremy, Tony Allen-Mills, Simon Wilde, D C, Martin James, Anthony Sattin, Robert Winnett, T E, Irwin Stelzer, Peter Wilson, David Dougill, Rachel Dobson, Peter Cook, High Canning, Phil Baty, Ann Corkett, H C, Cally Law, David Bond, Jonathon Carr-Brown, Alasdair Reid, Margarette Driscoll, Geraldine Hackett Education Correspondent, Nils Pratley, Jeremy Clarkson, David Cairns, Peter Olive, Edward Porter, Stewart Lee, Louise Taylor, Graham Dufill, S L, Victoria Segal, David Taylor, Patricia Nicol, Helen Kenny, Eben Black, John O'Donnell, R Plumb, Liz Edwards, Nelson Mandela, George Hart, Ruperrt Steiner, Adam Nathan, Stelios Haji-Loannou, Shelley von Strunckel, Jeremy Guscott, Anthony philiphs, Micheal Morpurgo, Jill Douglas, Richard Johnson, Peter Parker, Christopher Silvester, Stuart Wavell, Roger Eglin, Tom Conran, Dominique Coughlin, Glenn Gale, Vincent Crump, Hugh McIlvanney, Chris Woodhead, Jane Herald, Stan Labovitch, Lucinda Kemeny, Jasper Gerard, Paul Driver, Michael Woodhead, Garth Pearce, Ann McFerran, Maeve Sheehan, Edith Bowman, Richard Brooks, Ian Hawkey, Christopher Morgan, David Leppard, Denise Ryan, Paul Durman, Mark Ludlow, Adam Jolly, Alicia Wyllie, Mark Edwards, Simon Louvish, Louis de Bernierres, Mick Dennis, Susan Clark, Matthew Sheahan, Wendy Pott, W S Graff-Baker, Lucy Wakefield, Hugo Barnacle, Charlie Hedley, Michael Palin, Derek Wilcox, Victoria O'Brien, Marion Bull, Raymond Keene, Peter Martin, Kale Rew, Chris Robinson, Nils pratley, Gareth Huw Davies, Douglas Alexander, David Cracknell, Anthony ffrench-Constant, Roger Waters, Cosmo Landesman, Marie Colvin, Diana Wright, Peter Lampl, S B, Stephen Jones, Dominic O'Connell, S G, Louise Armitstead, Nick Cain, Henry Sterman, Graham Senior, Natalie Graham, C A Ryder, Bev Osborne, Caroline Donald, M J, Jessica Brinton, David Walsh, Jeremy Paxman, Steve Fitzgerald, John Stern, Jack Grimston, Colin McDowell, Lois Rogers Medical Correspondent, Stephen Armstrong, Sean Thomas, Ron Clarke, Elizabeth Jane Howard, Jackle Cooper, Colin McDowell's, Michael Sheridan Far East Correspondent, Sheherazade Goldsmith, Richard Woods, Roland White, Stephen magill, Robert White, Eddie Hawkins, Maurice Chittenden, Dave Stewart, Donna Tartt, Andrew Mueller, Jason Bell, Richard Brooks Arts Editor, Matthew Brace, Judith O'Reilly, Mark Franchetti, Richard Rae, Frank Fitzgibbon, Ray Hutton, Andy Warhol, Bridan Doogan, Andy Warnol, Ria Higgins, Jonathan Leake, Alexandra Dawe, M E, Leah Hardy, Tony Allen-Mills Independence, Michael Sheridan, Marathon, Lesley Cunliffe, Bikini Line, John Harlow, John Mortumer, Godfrey Smith, Barry Collins, Dipesh Gadher, Andrew Frankal, John Waples, Hunter Davies, Haruki Murakami, Ros Dodd, Matthew Campbell, Brian Glanville, Robert Steiner, Louise Johncox, John Carey, Martin Wroe, Tom Robbins, Mary Braid, John humphrys, Johan Schronen, jane mulkerrins, Jason Mellor, Lewis Stuart, Stephen Pettitt, Jonathan Calvert, John Mitchell, Justin Sparks, Sally Kinnes, Veronica Grookcock, Naomi Caine, Nigel Powell, Alan Thomas, David Wickers, Gill Wright, Caroline Turriff, Brian Doogan, John Aizlewood, Minette Marrin, Jeremy Lazell, Marcelle Katz, Mark Hodson, Graham Norwood, Chris Moore, Joe Lovejoy, Jonathan Futrell, Rupert Steiner, David Budworth, Hugh Pearman, Stephen Hird, Paul Stephen Lubicz, Dan Cairns, Christopher Bray, David Smith Economics Editor, Ben Rooney, Vanya Kewley, Niall Quinn, Anthony Howard, Sarah Baxter, Chris Feetenby, Matthew Goodman, Lord Macdonald, Steve Grant, Simon Howard, Danny Danziger, Jason Dawe, Lisa Verrico, Dominic Rushe, Barry Bolton,
... Items Multiple Display Advertising Items Debarnes Costa Blanca Cota del Sol Viva Estates Multiple Display Advertising Items ...
2002 - Gale Group | Sunday Times HA GDA
Silu Sheng, Edgardo T. Farinas,
... the reaction solution and reused. The laboratory evolved CotA laccase variant T480A-CotA was used to oxidize the following phenolic substrates: (+)- ... acid. The kinetic parameters were determined and T480A-CotA had a greater Vmax/Km than wt-CotA for all substrates. The Vmax/Km for T480A-CotA was 4.1, 5.6, and 1.4-fold greater than wt-CotA for (+)-catechin, (−)-epicatechin, and sinapic acid, respectively. The activity of wt-CotA and T480A-CotA was measured at different concentrations from 0–70% ... ethanol, methanol, and acetonitrile). The Vmax for T480A-CotA was observed to be greater than the wt- ...
Tópico(s): Enzyme Catalysis and Immobilization
2021 - Multidisciplinary Digital Publishing Institute | Catalysts
FROM OUR RACING CORRESPONDENT, FROM OUR NEWMARKET CORRESPONDENT, FROM OUR GOLF CORRESPONDENT, FROM OUR SPECIAL CORRESPONDENT, FROM OUR BOWLS CORRESPONDENT, FROM OUR MOTORING CORRESPONDENT, BASIL HOLMES, CAVAN, CREWE, CECIL HARMSWORTH, ESHER, JANET TREVELYAN, COTA., H. S. ALEXANDER., J. GILBERT WIBLIN, E. JEX-BLAKE., MAUD VON OSSIETZKY, ALBION BANERJI., ELENA C. DAVIES, RAYMOND SAVAGE., FROM OUR CORRESPONDENT, By CALLISTHENES, FROM OUR OWN CORRESPONDENT, FROM A CORRESPONDENT, FROM OUR CHESS CORRESPONDENT, From a Correspondent, CHARLES E. HOBHOUSE., B. E. GWYER, R. B. MOWAT., SHANE LESLIE, GILBERT JACKSON, GEORGE PAISH., From Our Labour Correspondent, VYVYAN ADAMS., FROM OUR MILITARY CORRESPONDENT, Our Naval Correspondent, BY E. T. MAC DERMOT, By Sir Robert Horne, K.C., M.P., By Rollo Appleyard, By Naomi Royde-Smith, By A. C. W. Lowe,
Births. Marriages. Deaths. Classified Advertising: Services To-Morrow, Transfer Books, Domestic Situations Vacant, Connoisseurs' Guide, Opera And Ballet, ...
1935 - Gale Group | TDA
Silu Sheng, Han Jia, Sidney W. Topiol, Edgardo T. Farinas,
... chaperones are present to assist in protein folding. CotA was optimized as a whole-cell biocatalyst immobilized ... stability using spore display. The maximum activity of CotA is between pH 4 and 5 for the ... was 24.8 times greater compared with wt-CotA. In a previous investigation, a CotA library was screened for organic solvent resistance and a T480A mutant was found. Consequently, T480A/E498G-CotA was constructed and the t1/2 was 62.1 times greater than wt-CotA. Finally, E498G-CotA and T480A/E498G-CotA yielded ...
Tópico(s): Transgenic Plants and Applications
2017 - Springer Science+Business Media | Journal of Microbiology and Biotechnology
Søren Brander, Jørn Dalgaard Mikkelsen, Kasper P. Kepp,
... the cloning, expression, and characterization of the bacterial cotA from Bacillus clausii, a supposed alkalophilic ortholog of cotA from B. subtilis. Both laccases were expressed in ... pH were similar, the activity of B. clausii cotA was shifted upwards by ∼0.5–2 pH ... of catalytic turnover: The kcat of B. clausii cotA was 1 s−1 at pH 6 and ... 1 at pH 8 for of B. subtilis cotA. Overall, kcat/KM was 10-fold higher for B. subtilis cotA at pHopt. While both proteins were heat activated, activation increased with pH and was larger in cotA from B. clausii. NaCl inhibited activity at acidic ...
Tópico(s): Analytical chemistry methods development
2014 - Public Library of Science | PLoS ONE
Jianmei Su, Peng Bao, Tenglong Bai, Lin Deng, Hui Wu, Fan Liu, Jin He,
... Mn(II) oxidases. Meanwhile, the endospore coat protein CotA from Bacillus species has been confirmed as a typical MCO. To study the relationship between CotA and the Mn(II) oxidation, the cotA gene from a highly active Mn(II)-oxidizing ... overexpressed in Escherichia coli strain M15. The purified CotA contained approximately four copper atoms per molecule and ... oxidases. Importantly, apart from the laccase activities, the CotA also displayed substantial Mn(II)-oxidase activities both ... II)-oxidizing activity. The specific activity of purified CotA towards Mn(II) was 0.27 U/mg. ...
Tópico(s): Chromium effects and bioremediation
2013 - Public Library of Science | PLoS ONE
Francisco J. Enguita, Lı́gia O. Martins, Adriano O. Henriques, M.A. Carrondo,
... thicker striated electrodense outer coat. The 65-kDa CotA protein is an abundant component of the outer coat layer. CotA is a highly thermostable laccase, assembly of which ... UV light. Here, we report the structure of CotA at 1.7-Å resolution, as determined by ... of a bacterial laccase. The overall fold of CotA comprises three cupredoxin-like domains and includes one ... Escherichia coli. However, the three cupredoxin domains in CotA are further linked by external interdomain loops, which ... multicopper oxidases containing four copper atoms suggest that CotA may accept the largest substrates of any known ...
Tópico(s): Biotin and Related Studies
2003 - Elsevier BV | Journal of Biological Chemistry
M. Sonoda, Ken Kitano, Akira Katoh, Hirokazu Katoh, Hiroshi Ohkawa, Teruo Ogawa,
cotA of Synechocystis sp. strain PCC6803 is a gene involved in light-induced proton extrusion (A. Katoh, ... 1996). There are two possible initiation codons in cotA, and either long (L-) or short (S-) cotA encoding a protein of 440 or 247 amino ... To determine the gene size, we inserted L-cotA and S-cotA into the genome of a cotA-less mutant (M29) to construct M29(L-cotA) and M29(S-cotA), respectively. M29(L-cotA) showed essentially the same net proton movement profile ...
Tópico(s): Protist diversity and phylogeny
1997 - American Society for Microbiology | Journal of Bacteriology
Yanrong Liu, Yongpeng Guo, Limeng Liu, Yu Tang, Yanan Wang, Qiugang Ma, Lihong Zhao,
... seriously threatens nutritional safety and common health. Bacterial CotA-laccases have great potential to degrade AFB1 without redox mediators. However, CotA-laccases are limited because of the low catalytic ... spore-bound nature. The AFB1 degradation ability of CotA-laccase from Bacillus licheniformis ANSB821 has been reported ... mutant was constructed to enhance the activity of CotA-laccase to degrade AFB1. After the site-directed ... kcat/Km) towards AFB1 than the wild-type CotA-laccase did. The degradation rate of AFB1 by ...
Tópico(s): Microbial Metabolism and Applications
2023 - Elsevier BV | Heliyon
Kai-Zhong Xu, Hui Ma, Yajing Wang, Yujie Cai, Xiangru Liao, Zheng-Bing Guan,
In this study, mutant CotA-laccase SF was successfully expressed in Escherichia coli by co-expression with phospholipase C. The optimized extracellular expression of CotA-laccase SF was 1257.22 U/L. Extracellularly expressed CotA-laccase SF exhibits enzymatic properties similar to intracellular CotA-laccase SF. CotA-laccase SF could decolorize malachite green (MG) under ... alkaline conditions. The Km and kcat values of CotA-laccase SF to MG were 39.6 mM ... transformed into 4-aminobenzophenone and 4-aminophenol by CotA-laccase. The toxicity experiment of garlic root tip ...
Tópico(s): melanin and skin pigmentation
2020 - Elsevier BV | Ecotoxicology and Environmental Safety
Lei Li, Tian Xie, Zhongchuan Liu, Hong Feng, Ganggang Wang,
CotA protein from Bacillus subtilis is of laccase activity. The solubility of recombinant CotA is low, which hinders its application. In this ... applied to increase the yield and activity of CotA protein. The results showed that the protein yield of CotA with his tag at C-terminal (CH6-CotA) was four times of that of NH6-CotA (His tag at N-terminal). Then, 23 single ... and three times higher than that of CH6-CotA, respectively. Finally, triple mutant F2071Y/V403T/P455S with ... and 42-folds compared to that of NH6-CotA in non-static culture.
Tópico(s): Microbial Metabolism and Applications
2017 - Oxford University Press | Protein Engineering Design and Selection
Yan‐Zhou Zhang, Xunhang Li, Zhikui Hao, Ruchun Xi, Yujie Cai, Xiangru Liao,
... should be exploited. In this study, H2O2-stable CotA and YjqC were isolated from the outer coat ... the thermal and alkali stability of catalytic activity, CotA also exhibited a much higher H2O2 tolerance than ... highly resistant to inhibition by H2O2 and heat. CotA could also catalyze the oxidation of SA and SNP. CotA had a much higher affinity for SA than B. subtilis CotA. CotA and YjqC rendered from B. altitudinis spores had ... regarded as a multi-enzyme biocatalyst composed of CotA and YjqC. The B. altitudinis spores were efficient ...
Tópico(s): Electrochemical sensors and biosensors
2016 - Public Library of Science | PLoS ONE
Sanam Islam Khan, Miray Şahinkaya, Dilşat Nigar Çolak, Numan Saleh Zada, Uğur Uzuner, Ali Osman Beldüz, Sabriye Çanakçı, Alam Khan, Samiullah Khan, Malik Badshah, Aamer Ali Shah,
... In the present study, spore coat A protein (CotA) encoding gene having laccase like activity from Bacillus altitudinis SL7 (CotA-SL7) was cloned and expressed in Escherichia coli. The purified CotA-SL7 was active at wide range of temperature ... and pH 5.0. The kinetic parameters of CotA-SL7 was determined with Km, Vmax, and kcat ... part in the oxidation of ABTS. The purified CotA-SL7 reduced lignin contents by 31 % and changes ... and opening of the benzene ring by purified CotA-SL7. Thus, high catalytic efficiency of CotA-SL7 ...
Tópico(s): Chromium effects and bioremediation
2023 - Elsevier BV | Enzyme and Microbial Technology
Jun Zeng, Qinghe Zhu, Yucheng Wu, Xiangui Lin,
... the oxidation of PAHs using another bacterial laccase CotA from Bacillus subtilis with a higher redox potential (525 mV) than CueO (440 mV). Recombinant CotA was overexpressed in E. coli and partially purified, ... CueO over a broad pH and temperature range. CotA exhibited moderate thermostability at high temperatures. CotA oxidized PAHs in the absence of exogenous copper. ... metal pollution can be avoided, another advantage of CotA over CueO. Moreover, this study also evaluated some ... mediators in crude cell extracts. Overall, the recombinant CotA oxidizes PAHs with high laccase activity and copper ...
Tópico(s): Pesticide and Herbicide Environmental Studies
2016 - Elsevier BV | Chemosphere
Sandeep Kumar, Kavish Kumar Jain, Kailash N. Bhardwaj, Subhojit Chakraborty, Ramesh Chander Kuhad,
... in a single host. Genes for bacterial laccase (CotA), pectate lyase (Pel) and endoxylanase (Xyl), which hold ... 1 vector containing two independent cloning sites (MCS). CotA and xyl genes were cloned in MCS1 and ... His tag and complete gene ORF) preceded by cotA open reading frame in the MCS1. IPTG induction ... of all three heterologous proteins of ~65 kDa (CotA), ~45 kDa (Pel) and ~25 kDa (Xyl), confirmed ... 16, ~720 and ~370 IU/ml activities of CotA, Pel and Xyl, respectively). Culture media optimization resulted ... 3 and 7 fold increased secretion of recombinant CotA, Pel and Xyl, respectively. Bioreactor level optimization of ...
Tópico(s): Biofuel production and bioconversion
2015 - Public Library of Science | PLoS ONE
Kathleen Sandman, Lee Kroos, Simon M. Cutting, Philip Youngman, Richard Losick,
The cotA (pig) gene of Bacillus subtilis encodes a 65,000 Mr protein that is a component of the ... undergoing sporulation. To study developmental regulation of the cotA gene, we identified its promoter and studied its ... analysis showed that induction and efficient transcription of cotA required DNA sequences extending no more than 55 ... 130 bp downstream) from the 5' terminus of cotA mRNA. Transcription from the cotA promoter was found to be switched on at ... the developmental stage (V) of spore coat formation. cotA transcription was also impaired in several stage V ...
Tópico(s): Enzyme Structure and Function
1988 - Elsevier BV | Journal of Molecular Biology
Yongpeng Guo, Xiaojuan Qin, Yu Tang, Qiugang Ma, Jianyun Zhang, Lihong Zhao,
In the present study, the CotA protein from Bacillus licheniformis ANSB821 was cloned and expressed in Escherichia coli. Apart from the laccase activities, we found that the recombinant CotA could effectively oxidize aflatoxin B1 in the absence ... Km, Kcat and Vmax values of the recombinant CotA towards aflatoxin B1 were 60.62 μM, 0. ... 10.08 μg min−1 mg−1, respectively. CotA-mediated aflatoxin B1 degradation products were purified and ... interaction played an important role in aflatoxin B1-CotA stability. These findings in the current study are ...
Tópico(s): Fungal and yeast genetics research
2020 - Elsevier BV | Food Chemistry
Juan Yang, Yaru Zhang, Shuo Wang, Shuna Li, Yiding Wang, Shuxiang Wang, LI Hong-ya,
... received increasing attention. Here, a recombinant bacterial laccase, CotA, from Bacillus amyloliquefaciens MN-13 was expressed in ... biocatalyst to degrade crystal violet (CV). The recombinant CotA remained stable at temperatures in the range 30– ... activity at pH 4.5–8.0. The CotA exhibited high activities for decolorization of CV and, after 72 h of incubation, CotA decolorized 70.98% of CV at pH 5. ... UV–visible spectra of CV solution treated by CotA, the full wavelength scan indicated that the chromophore ...
Tópico(s): Dye analysis and toxicity
2020 - Elsevier BV | Journal of Bioscience and Bioengineering
Jong-Hwa Park, Wooil Kim, Yong-Suk Lee, June-Hyung Kim,
In this study, we expressed cotA laccase from Bacillus subtilis on the surface of B. subtilis spores for efficient decolorization of synthetic dyes. The cotE, cotG, and ... anchoring motifs for efficient spore surface display of cotA laccase. Moreover, a His6 tag was inserted at the C-terminal end of cotA for the immunological detection of the expressed fusion protein. Appropriate expression of the CotE-CotA (74 kDa), CotG-CotA (76 kDa), and CotY-CotA (73 kDa) fusion proteins was confirmed by western ...
Tópico(s): Biotin and Related Studies
2019 - Springer Science+Business Media | Journal of Microbiology and Biotechnology
Alain R. Puente Santiago, Daily Rodríguez‐Padrón, Xuebo Quan, Mario J. Muñoz‐Batista, Lı́gia O. Martins, Sanny Verma, Rajender S. Varma, Zhou Jian, Rafael Luque,
... biosensors. In this work, ET rates of recombinant CotA laccases have been drastically improved by an immobilization ... on Sg-CN surfaces. In fact, the resulting CotA laccase/Sg-CN biomaterial displayed an ET rate ... all-atom molecular dynamics (AAMD) theoretical calculations proved CotA incorporation in a highly ordered array with an ... forward that takes into account the orientation of CotA laccase on the Sg-CN surface. Additionally, CotA laccase/Sg-CN biomaterial was tested as an ... emerging pollutants. In fact, the sensitivities of the CotA laccase/Sg-CN/ITO electrodes were 0.95 ...
Tópico(s): Advanced Nanomaterials in Catalysis
2018 - American Chemical Society | ACS Sustainable Chemistry & Engineering
Nadia A. Samak, Yeqiang Tan, Kunyan Sui, Tingting Xia, Kefeng Wang, Chen Guo, Chunzhao Liu,
... which have the ability to capture His-tagged CotA laccase was synthesized. Nα,Nα-Bis(carboxymethyl)-l- ... 49.9 and 145 mg/g-support, respectively. CotA laccase immobilized on MGO-NTA-Cu2+ nano-sheets ... sheets. The catalytic properties of MGO-NTA-Cu-CotA laccase were significantly improved in comparison with those of the free laccase. MGO-NTA-Cu-CotA laccase showed efficient decolorization for Congo Red (CR), ... C and pH 8. Furthermore, MGO-NTA-Cu-CotA laccase retained 89.4% of its initial activity ... MGO nano-sheets are promising support for efficient CotA laccase immobilization for environmental and industrial applications under ...
Tópico(s): Electrochemical sensors and biosensors
2017 - Elsevier BV | Molecular Catalysis
Han Jia, Frederick S Lee, Edgardo T. Farinas,
... The naturally occurring B. subtilis spore coat protein, CotA, was evolved for improved activity under conditions of high organic solvent concentrations. CotA is a laccase, which is a copper-containing oxidase enzyme. A CotA library was expressed on the spore coat, and ∼ ... 60% dimethyl sulfoxide (DMSO). A Thr480Ala variant (Thr480Ala-CotA) was identified that was 2.38-fold more active than the wild-type CotA. In addition, Thr480Ala-CotA was more active with different concentrations of DMSO ... be more active compared with the wild-type CotA in different concentrations of methanol, ethanol, and acetonitrile.
Tópico(s): bioluminescence and chemiluminescence research
2014 - American Chemical Society | ACS Combinatorial Science
G.Y. Park, Ning Hu, Xiaogang Wang, Ruxana T. Sadikot, Fiona E. Yull, Myungsoo Joo, R. Stokes Peebles, T.S. Blackwell, John W. Christman,
... transgenic mice, referred to as CycloOxygenase-2 TransActivated (COTA) mice, that overexpress a COX-2 transgene in ... 2 expression was increased in airway epithelium of COTA mice and whole lung tissue contained a three- ... F2α (PGF2α) compared to wild-type and untreated COTA mice. Interestingly, primary mouse tracheal epithelial cells from COTA mice produced only PGE2 by doxycycline-induced COX- ... interleukin (IL)-4 level in lung tissue from COTA mice compared to untreated COTA and wild-type mice. In addition, COTA mice ...
Tópico(s): Immune Response and Inflammation
2007 - Oxford University Press | Clinical & Experimental Immunology
Lı́gia O. Martins, Cláudio M. Soares, Manuela M. Pereira, Miguel Teixeira, Teresa Costa, George H. Jones, Adriano O. Henriques,
The Bacillus subtilis endospore coat protein CotA shows laccase activity. By using comparative modeling techniques, we were able to derive a model for CotA based on the known x-ray structures of ... oxidase and Cuprinus cereneus laccase. This model of CotA contains all the structural features of a laccase, ... and T3). Single amino acid substitutions in the CotA T1 copper center (H497A, or M502L) did not ... bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The CotA protein was purified to homogeneity from an overproducing ...
Tópico(s): Analytical chemistry methods development
2002 - Elsevier BV | Journal of Biological Chemistry
... found to display sequence homology with the cyanobacterial CotA product which is altered in mutants defective in ... have been shown to contain mutations within the cotA gene which displays significant sequence identity to ycf10 ( ... al., 1996a). More recently a role for the cotA product in light-induced proton extrusion has been ... cyanobacteria and plants. Cr, C.reinhardtii; S1 (slr1596) CotA, Synechocystis sp. PCC 6803 (Katoh et al., 1996a); ... 1990). The position of the initiator met of CotA (S1) proposed by Katoh et al. (1996a) is ...
Tópico(s): Protist diversity and phylogeny
1997 - Springer Nature | The EMBO Journal
C. C. Cheng, Anil Kumar Patel, Reeta Rani Singhania, Cheng-Hsian Tsai, Shen-Yi Chen, Chiu‐Wen Chen, Cheng‐Di Dong,
... MG removal. Study aims to increase laccase concentration, CotA-laccase from Bacillus subtilis was cloned and overexpressed in Escherichia coli. Optimal catalysis for purified CotA-laccase were at pH 5.0, 60 °C, ... 37.64 S-1 respectively. MG biodegradation by CotA-laccase in clam and tilapia pond wastewaters and ... fin-1 cells were determined. MG degradation by CotA-laccase was equally efficient, exhibiting upto 90–94% ... non-toxic to GF-1 cells. Thus, recombinant-CotA-laccase could be an environmentally-friendly enzyme for ...
Tópico(s): Environmental Toxicology and Ecotoxicology
2021 - Elsevier BV | Bioresource Technology
Kai-Zhong Xu, Haoran Wang, Yajing Wang, Jing Xia, Hui Ma, Yujie Cai, Xiangru Liao, Zheng-Bing Guan,
CotA-laccases are potential enzymes that are widely used in decolorization of dyes and degradation of toxic substances. In this study, a novel CotA-laccase gene from Bacillus pumilus W3 was applied ... catalytic efficiency (kcat/Km) than the wild-type CotA-laccase did. The optimal pH of S208G/F227A ... F227A was lower than that of wild-type CotA-laccase. This study showed that Gly208 and Ala227 ... it is possible to improve catalytic efficiency of CotA-laccase through site-directed mutagenesis.
Tópico(s): Microbial Metabolism and Applications
2019 - Elsevier BV | Journal of Bioscience and Bioengineering
Donna McNamara, Stuart L. Goldberg, Lisa Latts, Deena Graham, Stanley E. Waintraub, Andrew D. Norden, Cody Landstrom, Andrew L. Pecora, John G. Hervey, Eric Schultz, Ching-Kun Wang, Nicholas Jungbluth, Phillip M. Francis, Jane Snowdon,
... without reviewing the IBM Watson for Oncology with Cota RWE platform.The cognitive computing "recommended" option was ... were generated from 8% of cases. In the Cota observational database 69.3% of matched controls were ... recommended." Without guidance from Watson for Oncology (WfO)/Cota RWE, novice oncologists chose 75.5% recommended/for ... treatments which improved to 95.3% with WfO/Cota RWE. The novices were more likely than experts ... a non-recommended option (P < .01) without WfO/Cota RWE and changed decisions in 39% cases.Watson ...
Tópico(s): AI in cancer detection
2019 - Wiley | Cancer Medicine