Produção Nacional
Revisado por pares
Deborah Navit de Carvalho Cavalcante, Juliana Caroline Vivian Spósito, Bruno do Amaral Crispim, André Vieira do Nascimento, Alexéia Barufatti,
Nuclear abnormalities (micronuclei and meta-nuclear changes) have been used as biomarkers to identify cell damages. As children are more vulnerable to the adverse effects of pollution when compared to adults, assessing genetic damage caused by environmental influences is of great interest. As such, the objective was to determine metanuclear (karyolysis, pycnosis, karyorrhexis, binucleated cells, chromosome bridges and micronuclei) in cells from the oral mucosa of children associated with the school ...
Tópico(s): Effects and risks of endocrine disrupting chemicals
2017 - Taylor & Francis | Toxicology Mechanisms and Methods
Produção Nacional
Revisado por pares
Bruno do Amaral Crispim, Leonardo de Oliveira Seno, A. A. do Egito, Fernando Miranda de Vargas, Alexéia Barufatti,
Background:The aim of the present study was to assess the genetic diversity of Pantaneiro sheep, using microsatellite markers, in order to assist maintenance and management plans, enhance mating systems and reduce the inbreeding rate.A total of 127 animals were genotyped at eight microsatellite loci.They belonged to populations from the Experimental Farm of the Universidade Federal da Grande Dourados (UFGD) (Dourados/MS/Brazil) and Embrapa Pantanal (Corumbá/MS/Brazil).Results: The population of Pantaneiro ...
Tópico(s): Genetic Mapping and Diversity in Plants and Animals
2014 - Elsevier BV | Electronic Journal of Biotechnology
Produção Nacional
Revisado por pares
Jussara Oliveira Vaini, Bruno do Amaral Crispim, Danielly Beraldo dos Santos Silva, Celso Benites, Márcia Regina Russo, Alexéia Barufatti,
The presence of interspecific hybrid surubim (Pseudoplatystoma corruscans × Pseudoplatystoma reticulatum) in natural environments can put at risk the existence of the pure parental lineages. The aim of this study was to characterize the genetic variability of pure Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum individuals from rivers in the Paraná and Paraguay basins in Brazil. Seventy-six pure individuals of P. corruscans and 16 of P. reticulatum were evaluated with six microsatellite ...
Tópico(s): Fish Ecology and Management Studies
2016 - Springer Science+Business Media | Fisheries Science
Produção Nacional
Revisado por pares
Bruno do Amaral Crispim, Alexéia Barufatti, Leonardo de Oliveira Seno, A. A. do Egito, Fernando Miranda de Vargas, Mariana Rocha de Souza,
Sheep of the Pantaneiro breed and seven other breeds, raised in the State of Mato Grosso do Sul, Brazil, were genotyped using eight microsatellite loci.The aim of the present study was to determine the genetic variability, phylogenetic relationship, and patterns of gene introgression and miscegenation among the animals surveyed, to obtain information about the genetic structure of locally adapted sheep in Mato Grosso do Sul.A total of 195 animals were used for genetic analysis.The Pantaneiro breed ...
Tópico(s): Genetic Mapping and Diversity in Plants and Animals
2013 - Research Foundation of Ribeirão Preto | Genetics and Molecular Research
Produção Nacional
Revisado por pares
Luiza Flávia Veiga Francisco, Débora da Silva Baldivia, Bruno do Amaral Crispim, Syla Maria Farias Ferraz Klafke, Pamella Fukuda de Castilho, Lucilene Finoto Viana, Edson Lucas dos Santos, Kelly Mari Pires de Oliveira, Alexéia Barufatti,
The objective of this study was to use the same concentrations of aluminum (Al) and manganese (Mn) detected previously in groundwater above those permitted by Brazilian law and assess their cytotoxic and genotoxic effects in hamster ovary cell lines and their mutagenic effects through the Salmonella microsome assay. Chinese hamster ovary (CHO) and CHO-XRS5 cells were treated with different concentrations of Al and Mn (0.2 to 2.0 mg/L and 0.1 to 3.0 mg/L, respectively). The Ames test was used to analyze ...
Tópico(s): Heavy Metal Exposure and Toxicity
2021 - Multidisciplinary Digital Publishing Institute | Toxics
Produção Nacional
Revisado por pares
Luiza Flávia Veiga Francisco, Bruno do Amaral Crispim, Juliana Caroline Vivian Spósito, Júlio César Jut Solórzano, Nayara Halimy Maran, Fábio Kummrow, Valdir Aragão do Nascimento, Cassiana Carolina Montagner, Kelly Mari Pires de Oliveira, Alexéia Barufatti,
Tópico(s): Groundwater and Isotope Geochemistry
2019 - Springer Science+Business Media | Environmental Science and Pollution Research
Produção Nacional
Luiza Flavia Veiga Franscisco, Bruno do Amaral Crispim, Lucilene Finoto Viana, Hélina dos Santos Nascimento, Jorge Luiz Raposo, Alexéia Barufatti,
Metals are chemical contaminants that are not normally biodegradable and accumulate in living organisms. In addition, they may present toxic effects to plants and affect human health causing mutations and adn damage of exposed organisms. The objective of this study was to evaluate the cytotoxicity, genotoxicity and mutagenicity of the aluminum, manganese and lead using A.cepa test. Solutions in three concentrations with the metals and controls were prepared for the genetic analyzes. The seeds of ...
Tópico(s): Aluminum toxicity and tolerance in plants and animals
2018 - FUNDAÇÃO UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL | Orbital - The Electronic Journal of Chemistry
Produção Nacional
Revisado por pares
Bruno do Amaral Crispim, Jussara Oliveira Vaini, Alexéia Barufatti, Tatiane Zaratini Teixeira, Rosilda Mara Mussury, Leonardo de Oliveira Seno,
Tópico(s): Atmospheric chemistry and aerosols
2011 - Springer Science+Business Media | Environmental Science and Pollution Research
D.B.S. Silva, Bruno do Amaral Crispim, Ládia Eronildes Costa e Silva, Joyce Azambuja de Oliveira, F. Siqueira, Leonardo de Oliveira Seno, Alexéia Barufatti,
The aim of the study was to detect polymorphisms in the leptin gene and to determine the association of these polymorphisms with growth and carcass traits in Nellore cattle.The single nucleotide polymorphisms (SNPs) -1457 (AJ571671:g.-1457A>G)and A59V (AF536174.1:g.321C>T), as well as the microsatellite BM1500 (3.9 kb downstream), were genotyped.The measures of body weight and ultrasound examinations (rib eye area, back, and rump fat thickness) were performed in 3 different periods of animal management. ...
Tópico(s): Regulation of Appetite and Obesity
2014 - Research Foundation of Ribeirão Preto | Genetics and Molecular Research
Produção Nacional
Revisado por pares
Fábio Ederson Lopes Corrêa, Fabiana Gomes da Silva Dantas, Alexéia Barufatti, Bruno do Amaral Crispim, Kelly Mari Pires de Oliveira,
Introduction: The indiscriminate use of antimicrobials has selected for the emergence of resistant strains. Many mechanisms contribute to the spread of antimicrobial-resistant genes, and integrons play a key role in this process. The aim of this study was to describe the serotypes and resistance profiles, and to characterize the presence of integrons in Salmonella strains isolated from Dourados, Mato Grosso do Sul, Brazil. Methodology: Thirty-six isolates from different sources were used. To evaluate ...
Tópico(s): Fecal contamination and water quality
2014 - Open Learning on Enteric Pathogens | The Journal of Infection in Developing Countries
Produção Nacional
Revisado por pares
Bruno do Amaral Crispim, Juliana Caroline Vivian Spósito, Rosilda Mara Mussury, Leonardo de Oliveira Seno, Alexéia Barufatti,
Anatomical alterations in leaves and DNA damage in cells caused by the accumulation of atmospheric pollutants can be measured by epidermal leaf analyses and Tradescantia micronuclei assay with early pollen tetrad cells. The present study examined the feasibility of using somatic and germ cells of Tradescantia pallida for biomonitoring purposes in the city of Dourados, state of Mato Grosso do Sul (MS), Brazil. Stomatal, micronucleus and epidermal leaf analyses were performed, using standard methodologies, ...
Tópico(s): Plant responses to elevated CO2
2014 - Brazilian Academy of Sciences | Anais da Academia Brasileira de Ciências
Produção Nacional
Revisado por pares
Lucilene Finoto Viana, Yzél Rondon Súarez, Claúdia Andréa Lima Cardoso, Júlio César Jut Solórzano, Bruno do Amaral Crispim, Alexéia Barufatti, Sidnei Eduardo Lima‐Junior,
Tópico(s): Environmental Toxicology and Ecotoxicology
2017 - Springer Science+Business Media | Archives of Environmental Contamination and Toxicology
Produção Nacional
Revisado por pares
Juliana Caroline Vivian Spósito, Bruno do Amaral Crispim, Amanda Izadora Romãn, Rosilda Mara Mussury, Joelson Gonçalves Pereira, Leonardo de Oliveira Seno, Alexéia Barufatti,
In the present study, genotoxicity and mutagenicity were investigated in Tradescantia pallida exposed to vehicular traffic at different sites in a high-altitude tropical climate. During March, May, July, September, and November 2014, a comet assay and micronucleus bioassays were conducted on young inflorescences and leaves of T. pallida collected from twelve towns in the southern region of Mato Grosso do Sul with different amounts of vehicular traffic. Weather parameters (temperature, relative humidity ...
Tópico(s): Environmental Toxicology and Ecotoxicology
2017 - Elsevier BV | Chemosphere
Produção Nacional
Revisado por pares
Lucilene Finoto Viana, Yzél Rondon Súarez, Claúdia Andréa Lima Cardoso, Bruno do Amaral Crispim, Deborah Navit de Carvalho Cavalcante, Alexéia Barufatti, Sidnei Eduardo Lima‐Junior,
Tópico(s): Heavy metals in environment
2018 - Springer Science+Business Media | Archives of Environmental Contamination and Toxicology
Produção Nacional
Revisado por pares
Juliana Caroline Vivian Spósito, Luiza Flávia Veiga Francisco, Bruno do Amaral Crispim, Fabiana Gomes da Silva Dantas, Jéssica Pereira de Souza, Lucilene Finoto Viana, Júlio César Jut Solórzano, Kelly Mari Pires de Oliveira, Alexéia Barufatti,
Tópico(s): Environmental Toxicology and Ecotoxicology
2019 - Springer Science+Business Media | Archives of Environmental Contamination and Toxicology
Produção Nacional
Revisado por pares
José Darlan Alves da Silva, Cláudio Rodrigo Nogueira, Maria do Carmo Vieira, Silvia Cristina Heredia Vieira, Alexéia Barufatti, Bruno do Amaral Crispim, Luiza Flávia Veiga Francisco, Lucilene Finoto Viana, Claúdia Andréa Lima Cardoso,
ABSTRACT: The aqueous extract of Aristolochia triangularis leaves showed activity against Artemia salina larvae, with an LD50 of 370.6 µg/mL. In experiments with Allium cepa seeds, the extract caused a 51.26% reduction in the germination index, inhibited mean root growth, and was cytotoxic at concentrations of 668 and 2,000 µg/mL. Its antioxidant activity was additionally assessed in this research and the ferric reducing antioxidant power value was found to be 391.2 µM/g. Such health-beneficial ...
Tópico(s): Heavy Metals in Plants
2019 - UNIVERSIDADE FEDERAL DE SANTA MARIA | Ciência Rural
Produção Nacional
Revisado por pares
Silvana Lima dos Santos, Lucilene Finoto Viana, Felipe Mendes Merey, Bruno do Amaral Crispim, Júlio César Jut Solórzano, Alexéia Barufatti, Claúdia Andréa Lima Cardoso, Sidnei Eduardo Lima‐Junior,
Tópico(s): Heavy Metal Exposure and Toxicity
2020 - Elsevier BV | Chemosphere
Produção Nacional
Revisado por pares
Juliana Caroline Vivian Spósito, Bruno do Amaral Crispim, Rosilda Mara Mussury, Alexéia Barufatti,
To characterize the effect of vehicular traffic on air quality, the micronuclei of Tradescantia pallida tetrads were counted. Young inflorescences of T. pallida (Rose) D.R. Hunt var. purpurea were collected in 2010, 2011, and 2012, from three sites subjected to different intensities of vehicular traffic. The sites were located in the municipality of Dourados, in the State of Mato Grosso do Sul, Brazil. A standardized methodology was used to analyze the Tradescantia micronuclei, in order to evaluate ...
Tópico(s): Atmospheric chemistry and aerosols
2015 - Elsevier BV | Ecotoxicology and Environmental Safety
Produção Nacional
Revisado por pares
Adriana Araújo de Almeida-Apolonio, Bruno do Amaral Crispim, Alexéia Barufatti, Terezinha Inez Estivalet Svidzinski, Lais Gonçalvez Ortolani, Kelly Mari Pires de Oliveira,
In this study, the culture analysis of urine samples from patients hospitalized in the Central-West region of Brazil was performed, and the isolated microorganisms were phylogenetically identified as Trichosporon asahii. Maximum parsimony analysis of the IGS1 sequences revealed three novel genotypes that have not been described. The minimum inhibitory concentrations of the nine isolates identified were in the range of 0.06–1 μg/ml for amphotericin B, 0.25–4 μg/ml for fluconazole, and 0.03–0.06 μg/ ...
Tópico(s): Plant Pathogens and Fungal Diseases
2016 - Elsevier BV | Revista Argentina de Microbiología
Produção Nacional
Revisado por pares
Nayara Halimy Maran, Bruno do Amaral Crispim, Stephanie Ramirez Iahnn, Renata Pires de Araújo, Alexéia Barufatti, Kelly Mari Pires de Oliveira,
Use of groundwater from private wells in households has increased considerably, owing to a better cost/benefit ratio than that of water provided by local utilities for a fee. However, this water is usually untreated, which makes it a vehicle for diseases. Thus, monitoring this water is necessary to ensure its integrity and quality. We aimed to evaluate the physical, chemical, and microbiological parameters of untreated groundwater drawn from different types of wells, and the antimicrobial susceptibility ...
Tópico(s): Water Quality and Pollution Assessment
2016 - Multidisciplinary Digital Publishing Institute | International Journal of Environmental Research and Public Health
Produção Nacional
Revisado por pares
Fábio de Oliveira Roque, Angélica Guerra, Matthew Johnson, Carlos Roberto Padovani, Juliano José Corbi, Alan P. Covich, Donald P. Eaton, Walfrido Moraes Tomás, Francisco Valente‐Neto, Ana Cláudia Piovezan Borges, Alexandra De Pinho, Alexeia Barufatii, Bruno do Amaral Crispim, Rafael Dettogni Guariento, Maria Helena da Silva Andrade, Ary T. Rezende-Filho, Rodolfo Portela, Marcia Divina, Julio César Sampaio da Silva, Cássio Bernadino, Érica Fernanda Gonçalves Gomes de Sá, Pedro Cordeiro Estrela de Andrade Pinto, Arnaud Léonard Jean Desbiez, Isabel M.D. Rosa, Lisa Yon,
As a consequence of accelerated and excessive use of pesticides in tropical regions, wilderness areas are under threat; this includes the Pantanal wetlands in the Upper Paraguay River Basin (UPRB). Using a Land Cover Land Use Change (LCLUC) modelling approach, we estimated the expected pesticide load in the Pantanal and the surrounding highlands region for 2050 under three potential scenarios: i) business as usual (BAU), ii) acceleration of anthropogenic changes (ACC), and iii) use of buffer zones around ...
Tópico(s): Conservation, Biodiversity, and Resource Management
2021 - Elsevier BV | Agriculture Ecosystems & Environment
Produção Nacional
Revisado por pares
Lucilene Finoto Viana, Fábio Kummrow, Claúdia Andréa Lima Cardoso, Nathalya Alice de Lima, Júlio César Jut Solórzano, Bruno do Amaral Crispim, Alexéia Barufatti, Alexandro Cezar Florentino,
The Araguari River is one of the most important water body in the Brazilian state of Amapá. However, the quality of its waters has been deteriorating and, recently, high concentrations of metals were found in water samples collected from its lower section. Overall, we aimed to evaluate land use and cover around three sampling sites located in the Araguari River lower section and its contribution to water pollution by metals; correlate land use pattern with detected metal concentrations; assess acute ...
Tópico(s): Mercury impact and mitigation studies
2021 - Elsevier BV | Chemosphere
Produção Nacional
Bruno do Amaral Crispim, Danielly Beraldo dos Santos Silva, Alexandre Campos Banari, Leonardo de Oliveira Seno, Alexéia Barufatti,
As técnicas de biologia molecular que são utilizadas na discriminação alélica para caracterização individual e de raças de ovinos são ferramentas importantes nos programas de melhoramento genético e de conservação de recursos genéticos.A utilização de marcadores moleculares microssatélites possibilita a diferenciação alélica, que por sua vez permite inferir a variabilidade genética de populações amostrais.O estudo teve como objetivo testar a sensibilidade e a eficiência da eletroforese capilar fluorescente, ...
Tópico(s): Genetic and phenotypic traits in livestock
2012 - Selva Andina Research Society | Journal of the Selva Andina Research Society
Produção Nacional
Revisado por pares
Lucilene Finoto Viana, Yzél Rondon Súarez, Claúdia Andréa Lima Cardoso, Bruno do Amaral Crispim, Alexéia Barufatti, Sidnei Eduardo Lima‐Junior,
Tópico(s): Heavy Metal Exposure and Toxicity
2017 - Springer Science+Business Media | Environmental Science and Pollution Research
Produção Nacional
Revisado por pares
Hélina dos Santos Nascimento, Bruno do Amaral Crispim, Luiza Flávia Veiga Francisco, Felipe Mendes Merey, Fábio Kummrow, Lucilene Finoto Viana, L. A. K. A. Inoue, Alexéia Barufatti,
The use of non-plant and plant-derived anesthetics in aquaculture activities is a frequent and necessary practice to maintain fish welfare. Currently anesthetics authorized for use in aquaculture activities are limited, and the use of unauthorized products is common. However, to obtain authorization for use in aquaculture and ensure their safety, anesthetics should be evaluated for their genotoxic potential. Our objective was to evaluate the genotoxicity of three not approved anesthetics commonly ...
Tópico(s): Pharmaceutical and Antibiotic Environmental Impacts
2020 - Elsevier BV | Aquaculture Reports
Produção Nacional
Revisado por pares
Jéssica Pereira de Souza, Juliana Caroline Vivian Spósito, Bruno do Amaral Crispim, Fabiane Gomes da Silva, Kelly Mari Pires de Oliveira, Fábio Kummrow, Valdir Aragão do Nascimento, Cassiana Carolina Montagner, Lucilene Finoto Viana, Júlio César Jut Solórzano, Alexéia Barufatti,
Tópico(s): Pharmaceutical and Antibiotic Environmental Impacts
2019 - Springer Science+Business Media | Ecotoxicology
Produção Nacional
Revisado por pares
Joyce Azambuja de Oliveira, Camila Magalhães da Cunha, Bruno do Amaral Crispim, Leonardo de Oliveira Seno, Alexandre Rodrigo Mendes Fernandes, Guilherme de Paula Nogueira, Alexéia Barufatti,
Advances in DNA technology have created biotechnological tools that can be used in animal selection and new strategies for increasing herd productivity and quality. The objective of the present work was to associate the genotypes of leptin gene exon 2 polymorphisms with productive traits in Nellore cattle. Blood was collected from Nellore males and PCR-RFLP reactions were performed with the restriction enzymes Cla I and Kpn 2I. The gene frequencies resulting from digestion by Cla I were 0.60 and ...
Tópico(s): Regulation of Appetite and Obesity
2013 - Taylor & Francis | Animal Biotechnology
Produção Nacional
Revisado por pares
Lucilene Finoto Viana, Fábio Kummrow, Claúdia Andréa Lima Cardoso, Nathalya Alice de Lima, Bruno do Amaral Crispim, Alexéia Barufatti, Alexandro Cezar Florentino,
Tópico(s): Heavy Metal Exposure and Toxicity
2022 - Springer Science+Business Media | Environmental Science and Pollution Research
Produção Nacional
Revisado por pares
Lucilene Finoto Viana, Bruno do Amaral Crispim, Fábio Kummrow, Nathalya Alice de Lima, Mariana Amaral Dias, Cassiana Carolina Montagner, Ricardo Henrique Gentil Pereira, Adriana Lúcia de Escobar Chaves de Barros, Alexéia Barufatti,
The Aquidauana River is an important ecological corridor in the Pantanal biome. However, the growth of agricultural and urban areas along its banks has contributed to the deterioration of its water quality, consequently putting the aquatic biota at risk. Our objectives were to evaluate: 1) the composition of the landscape around six sampling sites located in the Aquidauana River middle section; and 2) the quality of its water by determining limnological parameters, concentrations of contaminants ...
Tópico(s): Aquatic Ecosystems and Phytoplankton Dynamics
2023 - Elsevier BV | Chemosphere
Produção Nacional
Revisado por pares
Lucilene Finoto Viana, Bruno do Amaral Crispim, Juliana Caroline Vivian Spósito, Milena Perez de Melo, Luiza Flávia Veiga Francisco, Valter Aragão do Nascimento, Alexéia Barufatti,
This study assessed the concentrations of metals in the Dourados and Brilhante Rivers, investigated the influence of landscape features surrounding these rivers on water quality, and evaluated the risks associated with metal contamination for aquatic biota and human health. Sampling was conducted at eighteen sites distributed along the rivers in June 2016. The concentrations of metals (Cu, Zn, Mn, Fe, Cr, Al and Co) in the water were analyzed by inductively coupled plasma optical emission spectroscopy. ...
Tópico(s): Heavy metals in environment
2021 - Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHi) | Ambiente e Agua - An Interdisciplinary Journal of Applied Science