Yingjun Zhao, Yunshu Wang, Jin Hu, Xian Zhang, Yun‐wu Zhang,
... affected by the subcellular localization of these factors. CUTA, the mammalian CutA divalent cation tolerance homolog (E. coli), has been ... Alzheimer disease pathology. However, the exact function of CUTA remains largely unclear. Here we show that human CUTA has several variants that differ in their N- ... Importantly, we demonstrate that the H component of CUTA interacts through its N terminus with the transmembrane ... Golgi network. Overexpression and RNA interference knockdown of CUTA can reduce and increase BACE1-mediated APP processing/ ...
Tópico(s): Prion Diseases and Protein Misfolding
2012 - Elsevier BV | Journal of Biological Chemistry
Ping Hou, Guiying Liu, Yingjun Zhao, Zhun Shi, Qiuyang Zheng, Guojun Bu, Huaxi Xu, Yun‐wu Zhang,
... Recently, we find that a copper-related protein, CutA divalent cation tolerance homolog of Escherichia coli (CUTA), interacts with the β-secretase β-site APP ... generation. Herein, we further found that overexpression of CUTA increases intracellular copper level, whereas copper treatments promote CUTA expression. We also confirmed that copper treatments promote ... promoted the increase of Aβ secretion induced by CUTA downregulation but had no effect on CUTA-β-site APP cleaving enzyme 1 interaction. On ...
Tópico(s): Prion Diseases and Protein Misfolding
2014 - Elsevier BV | Neurobiology of Aging
Khaled A. Selim, Lorena Tremiño, Clara Marco‐Marín, Vikram Alva, Javier Espinosa, Asunción Contreras, M.D. Hartmann, Karl Forchhammer, Vicente Rubio,
The PII‐like protein CutA is annotated as being involved in Cu 2+ tolerance, based on analysis of Escherichia coli mutants. However, the precise cellular function of CutA remains unclear. Our bioinformatic analysis reveals that CutA proteins are universally distributed across all domains of ... on sequence‐based clustering, we chose representative cyanobacterial CutA proteins for physiological, biochemical, and structural characterization and ... their involvement in heavy metal tolerance, by generating CutA mutants in filamentous Nostoc sp. and in unicellular ...
Tópico(s): Porphyrin Metabolism and Disorders
2020 - Wiley | FEBS Journal
Alexei Savchenko, T. Skarina, E. Evdokimova, James D. Watson, Roman A. Laskowski, C.H. Arrowsmith, A.M. Edwards, A. Joachimiak, Rong‐guang Zhang,
The structure of the CutA protein from Thermotoga maritima (tmCutA) was determined at 1.4 Å resolution using the Se-Met multiwavelength anomalous diffraction (MAD) ... including plants and mammals (COG1324, Fig. 1). The CutA Escherichia coli homolog—CutA1 (35% ID) is involved ... acetylcholinesterase.2 However, the biological function of the CutA proteins is yet to be determined. Multiple sequence alignment of CutA from Thermotoga maritima (Thema) against other periplasmic divalent ... none. The secondary structure of the T. maritima CutA is schematically shown in purple above the alignment. ...
Tópico(s): Drug Transport and Resistance Mechanisms
2003 - Wiley | Proteins Structure Function and Bioinformatics
Produção Nacional
Revisado por pares
Dong Liang, Nilson Nunes‐Tavares, Heidi Q. Xie, Stéphanie Carvalho, Suzanne Bon, Jean Massoulié,
The mammalian protein CutA was first discovered in a search for the membrane anchor of mammalian brain acetylcholinesterase (AChE). It was co-purified ... distinct from the real transmembrane anchor protein, PRiMA. CutA is a ubiquitous trimeric protein, homologous to the ... the expression and the subcellular localization of mouse CutA variants, starting at three in-frame ATG codons, in transfected COS cells. We show that CutA produces 20-kDa (H) and 15-kDa (L) ... cytosolic. We show that expression of the longer CutA variant reduces the level of AChE, that this ...
Tópico(s): Insect Resistance and Genetics
2008 - Elsevier BV | Journal of Biological Chemistry
Fuyu Ito, Kengo Usui, Daigo Kawahara, Atsushi Suenaga, Tei Maki, Satoru Kidoaki, Harukazu Suzuki, Makoto Taiji, Masayoshi Itoh, Yoshihide Hayashizaki, Takehisa Matsuda,
... fused to each end of the triangular trimeric CutA protein (CutA-TIP1), and a PDZ domain-recognizable peptide which ... a cell-adhesive RGD tripeptidyl sequence in the CutA loop region [CutA(RGD)-TIP1]. Spontaneous viscoelastic hydrogel formation occurred when either CutA-TIP1- or CutA(RGD)-TIP1-containing buffer solution and PDZ-peptide- ... presence or absence of RGD motif in the CutA-TIP1 molecule. The potential use of such a ...
Tópico(s): Cell Adhesion Molecules Research
2009 - Elsevier BV | Biomaterials
Yoshikazu Tanaka, Kouhei Tsumoto, Takeshi Nakanishi, Y. Yasutake, Naoki Sakai, Min Yao, Isao Tanaka, Izumi Kumagai,
CutA is a small protein that appears to be involved in the mechanism of divalent metal cation ... we report the crystal structure of Pyrococcus horikoshii CutA ( Pho CutA), with and without Cu 2+ , and its metal‐binding properties. Crystallographic analyses revealed that Pho CutA forms a stable trimeric structure with intertwined antiparallel ... The crystal structure of the Cu 2+ – Pho CutA complex shows that the Cu 2+ is located ... each trimer. In an in vitro experiment, Pho CutA bound to several heavy metals, most of which ...
Tópico(s): Enzyme Structure and Function
2003 - Wiley | FEBS Letters
Xiaodi Yu, Shiqiang Chai, Xiaojun Kuang, Congxue Su, Fengjuan Pan, Liang Fang, Qiang Su,
Ba 8 CuTa 6 O 24−δ ceramics possess exceptionally high microwave dielectric loss among the eight‐layer twinned hexagonal perovskite ... measurement demonstrates that the eight‐layer Ba 8 CuTa 6 O 24−δ ceramics show the electrical ... barrier layer capacitance (IBLC) effects on Ba 8 CuTa 6 O 24−δ ceramics. The heterogeneous electrical ... cooling. The existence of Cu + in Ba 8 CuTa 6 O 24−δ ceramic is confirmed by ... leaky insulating bulk property for the Ba 8 CuTa 6 O 24−δ ceramics is compared with ... high microwave dielectric loss of the Ba 8 CuTa 6 O 24−δ ceramics.
Tópico(s): Ferroelectric and Piezoelectric Materials
2013 - Wiley | Journal of the American Ceramic Society
Sheik‐Tao Fong, James Camakaris, Barry T. O. Lee,
Summary The cutA locus, presumably involved in copper tolerance in Escherichia coli , was characterized by a mutation leading to copper sensitivity. ... radioactive 64 Cu 6+ showed increased uptake by cutA copper‐sensitive mutant cells, and reduced uptake when the cutA mutation was complemented in trans. The locus was mapped using complementation of the cutA mutant to partial copper tolerance with wild‐type ... The 3.2 kb DNA region involved in cutA was sequenced and analysed, revealing three significant open ...
Tópico(s): Salmonella and Campylobacter epidemiology
1995 - Wiley | Molecular Microbiology
Chao Gu, Wang Fei, Ping Huang, T.J. Lu, Kewei Xu,
... are performed on sputtering deposited mono layered nanocrystalline CuTa and multilayered CuTa/Cu thin films. Nanoindentation hardness, microstructural features and ... which is closely related to the structure of CuTa layer. Further, by adding Cu layers, both strengthening and softening effects are observed in CuTa/Cu thin films with different Ta content but ... Based on experimental results, dominant deformation mechanisms of CuTa and CuTa/Cu thin films having various Ta content are ...
Tópico(s): Microstructure and mechanical properties
2015 - Elsevier BV | Materials Science and Engineering A
Chao Gu, Wang Fei, Ping Huang, K.W. Xu, T.J. Lu,
CuTa monolayers with different Ta contents (34 at% and 37 at%) and CuTa/Cu multilayers with varying nanoscale Cu layer thickness ( ... nanoparticles. Adding Cu layers into the two different CuTa monolayers led to CuTa-Cu interfaces with different microstructures. For CuTa34/Cu, ... on multilayer deformation, the residue indentation morphologies of CuTa/Cu were observed under scanning electronic microscope (SEM). ... and size effects were proposed and discussed for CuTa/Cu thin films possessing different interfaces and Cu ...
Tópico(s): Adhesion, Friction, and Surface Interactions
2016 - Elsevier BV | Materials Science and Engineering A
Amin Bahrami, C. Carrasco, Arturo Delgado Cardona, Teodor Huminiuc, Tomáš Polcar, Sandra E. Rodil,
... of as-deposited and 823.15 K annealed CuTa coatings were studied with the purpose to evaluate ... stability as a function of the Ta content. CuTa coatings with a Ta content from 0 to ... The XRD results show that a Ta-rich CuTa glass metal is formed in the coatings with ... formation of crystalline Cu islands in an amorphous CuTa matrix in both as-deposited and annealed coatings. ... 3D distribution of Cu nanocrystals within an amorphous CuTa matrix which might reduce the brittleness of the glassy CuTa matrix. A significant increase in hardness values from ...
Tópico(s): Metal and Thin Film Mechanics
2019 - Elsevier BV | Surface and Coatings Technology
Junrong Wang, Luying Qiao, Guo‐Qing Zhu, Qianqian Sun, Yulin Xie, Man Wang, Yaqi Xu, Chunxia Li,
... activation nanoneedle for pyroptosis induction, copper-tannic acid (CuTA), was synthesized and combined with the sonosensitizer Chlorin e6 (Ce6) to form a pyroptosis amplifier (CuTA-Ce6) for dual activation and amplification of pyroptosis ... SDT) further enhanced the cellular pyroptosis caused by CuTA, activating the body to develop a powerful anti-tumor immune response. Concretely, CuTA nanoneedles with quadruple mimetic enzyme activity could be ... effects. Considering the outstanding oxygen-producing capacity of CuTA and the distinctive advantages of US, the sonosensitizer ...
Tópico(s): Inflammasome and immune disorders
2024 - Elsevier BV | Journal of Controlled Release
Yingying Xu, Yifan Luo, Zhenzhen Weng, Haichang Xu, Wei Zhang, Qun Li, Huijie Liu, Lubing Liu, Yanmei Wang, Xuexia Liu, Lan Liao, Xiaolei Wang,
... tissues. Herein, we have proposed the TM/BHT/CuTA hydrogel system formed by the self-assembly of ... copper-based nanozyme (copper tannic acid coordination nanosheets, CuTA NSs) and the triglycerol monostearate/2,6-di- ... TM/BHT) hydrogel. The negatively charged TM/BHT/CuTA can retain at the inflammation sites with a ... periodontitis, realizing the on-demand release of the CuTA nanozyme. The released CuTA nanozyme has antibacterial and antiplaque properties. Meanwhile, as ... superoxide dismutase (SOD) and catalase (CAT). Further, the CuTA nanozyme can modulate the macrophage polarization from M1 ...
Tópico(s): Oral microbiology and periodontitis research
2023 - American Chemical Society | ACS Nano
Jia Li, Jingyu Li, Jiayu Wei, Xiaobo Zhu, Shihui Qiu, Haichao Zhao,
The copper tannic acid (CuTA) nanosheets with an excellent antibacterial activity were successfully prepared, which showed fine antibacterial and antifouling performance after hybridization with acrylic resin. The morphology and structure characterization of CuTA nanosheets were studied by transmission electron microscopy, scanning ... of 2 and 0.5 mg/mL of CuTA powder were close to 100% after 24 h. ... and element distribution of bacteria, after treating with CuTA powder, revealed that Cu2+ and TA destroyed their ...
Tópico(s): Polymer Surface Interaction Studies
2021 - American Chemical Society | ACS Applied Materials & Interfaces
Zhicheng Cao, Hongmei Wang, Jialin Chen, Yanan Zhang, Qingyun Mo, Po Zhang, Mingyue Wang, Haoyang Liu, Xueyang Bao, Yuzhi Sun, Wei Zhang, Qingqiang Yao,
... based hydrogel incorporated with metal-organic framework nanozymes (CuTA@SF) to provide a suitable microenvironment for enhanced OCD regeneration. The incorporation of CuTA nanozymes endowed the SF hydrogel with a uniform ... mesenchymal stem cells (MSCs) and chondrocytes showed that CuTA@SF hydrogel accelerated cell proliferation and enhanced cell ... inflammatory environment with the stimulation of IL-1β, CuTA@SF hydrogel still possessed the potential to promote ... matrix (ECM). The proteomics analysis further confirmed that CuTA@SF hydrogel promoted cell proliferation and ECM synthesis. ...
Tópico(s): Nanoplatforms for cancer theranostics
2022 - Elsevier BV | Bioactive Materials
Fabio Arnesano, Lucia Banci, Manuela Benvenuti, Ivano Bertini, V. Calderone, Stefano Mangani, Maria Silvia Viezzoli,
... in a gene locus of Escherichia coli called cutA involved in divalent metal tolerance (1Fong S.T. ... 1127-1137Crossref PubMed Scopus (98) Google Scholar). The cutA locus consists of two operons, one containing a ... proteins. Molecular genetics studies on the E. coli cutA locus showed that some mutations lead to copper ... by one of the three genes of the cutA locus, which is involved in tolerance to Cu2+ ... Supplemental Fig. 1. The second gene of the cutA locus encodes the transmembrane electron transporter CutA2. A ...
Tópico(s): Cellular Mechanics and Interactions
2003 - Elsevier BV | Journal of Biological Chemistry
Jingchun Yang, Qiang Li, Huirong Yang, Lichong Yan, Yang Liu, Long Yu,
... involved in pathological events of many diseases. Human CUTA isoform2 is a member of cation tolerance protein ( ... In this study, we examined the effect of CUTA isoform2 overexpression on copper toxicity. It was shown that overexpressed CUTA isoform2 sensitized HeLa cells to copper toxicity by ... on cell proliferation was also enhanced by overexpressed CUTA isoform2. So CUTA isoform2 was implicated to be involved in the ...
Tópico(s): Neurological diseases and metabolism
2008 - Polish Biochemical Society | Acta Biochimica Polonica
Ross Crowhurst, Stephanie J. Binnie, Joanna K. Bowen, B. T. Hawthorne, Kim M. Plummer, J. Rees‐George, Erik H. A. Rikkerink, Matthew D. Templeton,
... and 98% identity, respectively, to those of the cutA gene of the pea pathogen F. solani f. ... replacement vector was constructed and used to generate cutA - mutants that were detected with a polymerase chain reaction (PCR) assay. Seventy-one cutA - mutants were identified among the 416 transformants screened. ... of homologous integration was 14%. Disruption of the cutA locus in mutants was confirmed by RNA gel ... found to be affected by disruption of the cutA gene.
Tópico(s): Plant tissue culture and regeneration
1997 - American Phytopathological Society | Molecular Plant-Microbe Interactions
Margarett Shnorhavorian, Rachel Bittner, Jonathan L. Wright, Stephen M. Schwartz,
... maternal risk factors for congenital urinary tract anomalies (CUTA) are limited, with conflicting results for preexisting diabetes ... examine the association between maternal risk factors and CUTA in a population-based case-control study.A ... evaluate the association between maternal risk factors and CUTA. Cases were children with International Classification of Diseases, ... to evaluate the association between maternal factors and CUTA.A total of 4673 cases and 18,692 ... maternal factors are associated with the risk of CUTA, including diabetes mellitus and maternal renal disease. This ...
Tópico(s): Birth, Development, and Health
2011 - Elsevier BV | Urology
Kamil Kobyłecki, Krzysztof Kuchta, Andrzej Dziembowski, Krzysztof Ginalski, Rafał Tomecki,
... domain (SBD) with inserted NTase domain. Aspergillus nidulans CutA (AnCutA), synthesizes C/U-rich 3'-terminal extensions ... the 3'-RACE products for tails generated by CutA proteins in vitro in the presence of all ... after adding two cytidines only. Comparison of our CutA 3D model to selected noncanonical NTases of known ... NRM) within PAP/OAS1 SBD. We demonstrate that CutA specificity toward CTP can be partially changed to ...
Tópico(s): Ubiquitin and proteasome pathways
2017 - Cold Spring Harbor Laboratory Press | RNA
Shichao Lin, Yuan Cheng, He Zhang, Xiaoyu Wang, Yuye Zhang, Yuanjian Zhang, Leiying Miao, Xiaozhi Zhao, Hui Wei,
... challenge, herein, a novel copper tannic acid coordination (CuTA) nanozyme is reported as a highly active and thermostable ROS scavenger. The CuTA nanozyme exhibits intrinsic superoxide dismutase‐like activity, catalase‐ ... elimination capacity. These synergistic antioxidant abilities make the CuTA nanozyme a promising candidate for the improvement of ... model results show that commercial cigarettes loaded with CuTA nanozyme efficiently scavenge ROS in the cigarette smoke, ... minimize the resultant acute lung injury. The developed CuTA nanozyme offers an efficient ROS scavenger with multiple ...
Tópico(s): Nanoplatforms for cancer theranostics
2019 - Wiley | Small
Produção Nacional
Revisado por pares
João Batista Santos Garcia, Letácio Santos Garcia Ferro, Anamada Barros Carvalho, Rosyane Moura da Rocha, Livia Maria Lima de Souza,
... anticonvulsivante bem tolerado, porém frequentemente associado a reações cutâ neas graves, como, por exemplo, a síndrome de ... foi internada com urticária e exantema generalizados, erupções cutâ neas eritematosas, bolhosas e máculas purpúricas por todo ... aumento do número e do tamanho das lesões cutâ neas, além de rash eritematoso macular generalizado, áreas ... a óbito. A SSJ/NET é uma reação cutâ nea grave com potencial para morbidade e mortalidade ...
Tópico(s): Urticaria and Related Conditions
2010 - Elsevier BV | Brazilian Journal of Anesthesiology
J.A.L. van Kan, J.W. van t Klooster, C.A.M. Wagemakers, D. Dees, C.J.B. van der Vlugt-Bergmans,
... study the expression of the cutinase encoding gene cutA of B. cinerea was analyzed using a cutA promoter-GUS reporter gene fusion. Transformants containing the ... and during penetration into epidermal cells, indicating that cutA is expressed during the early stages of infection. ... disruption. Pathogenicity of two transformants lacking a functional cutA gene was studied on gerbera flowers and tomato ...
Tópico(s): Insect Resistance and Genetics
1997 - American Phytopathological Society | Molecular Plant-Microbe Interactions
A. Arunjegan, Rajaji Pavadai, S. Sivanesan, Panneerselvam Perumal,
... i.e., nano-monoclinic copper-tannic acid (NMc-CuTA)). An exploration of the sensing mechanism shows that LFGr-ssDNA and AO strongly adsorb on NMc-CuTA through π-π stacking and electrostatic interactions, and ... which was released from the surface of NMc-CuTA with strong AO fluorescence enhancement (Turn-ON). The ... Pb2+ and Epn, respectively. Hence, the reported NMc-CuTA-based sensing platform has potential applications for the ...
Tópico(s): Biosensors and Analytical Detection
2021 - Royal Society of Chemistry | RSC Advances
Shengye You, Yajing Xiang, Xiaoliang Qi, Ruiting Mao, Erya Cai, Yulong Lan, Hongyang Lu, Jianliang Shen, Hui Deng,
... a conveniently multifunctional hydrogel wound dressing (abbreviated as CuTA/SG) was constructed by incorporating copper/tannic acid ... oxidized sodium alginate, gelatin, and Ca2+. The resulting CuTA/SG hydrogel had excellent exudate absorption, adjustable photothermal antibacterial, and ROS scavenging features. Furthermore, CuTA/SG hydrogel was injectable that possessed excellent adaptability ... due to its appropriate physical cross-linking. Astonishingly, CuTA/SG hydrogel showed good hemostasis, antibacterial, anti-inflammation, ...
Tópico(s): Electrospun Nanofibers in Biomedical Applications
2022 - Elsevier BV | Materials Today Advances
Jahanghir Azizi, Siamak Javanbakht, Réza Mohammadi,
... the preparation of a copper tannic acid MOF (CuTA) and graphene quantum dot (GQD) nanocomposite, GQD/CuTA, by a simple, environmentally friendly, and inexpensive method ... and followed by they effectively anchor MOF nanoparticles (CuTA) by a green hydrothermal in situ synthesizing method. The constructed GQD/CuTA nanomaterials were characterized and validated using XRD, FT- ... antibacterial activity against E. coli bacteria for both CuTA and GQD/CuTA (0 CFU/mL) and S. aureus bacteria for CuTA and GQD/CuTA (300 and 40 CFU/mL) ...
Tópico(s): Nanocluster Synthesis and Applications
2023 - Elsevier BV | International Journal of Pharmaceutics
... of liquid $^{3}\mathrm{He}$ in contact with CuTA [copper tetrammine sulfate monohydrate, Cu${(\mathrm{N}{\mathrm{ ... temperature, ${T}_{\mathrm{N}}=0.43$ K, of CuTA. Data show that the electron-spin fluctuation in CuTA plays an important role for the relaxation mechanism ...
Tópico(s): NMR spectroscopy and applications
1976 - American Physical Society | Physical Review Letters
Marcella Zollino, Fiorella Gurrieri, Daniela Orteschi, Giuseppe Marangi, Vincenzo Leuzzi, Giovanni Neri,
... syndromic mental retardation, and of the flanking genes CuTA, a likely modulator of the processing and trafficking ... involved in HOX gene silencing. Mutations of both CuTA and hPHF1 were never reported as causative of ... interval. In addition, experimental evidence that SYNGAP1 and CuTA are involved in the secretory pathway in neurons, ...
Tópico(s): Hedgehog Signaling Pathway Studies
2010 - Springer Nature | European Journal of Human Genetics
Igor Y. Morozov, Meriel G. Jones, David G. Spiller, Daniel J. Rigden, Christoph Dattenböck, René Novotny, Joseph Strauss, Mark X. Caddick,
... deleted for Edc3, an enhancer of decapping, or CutA, which encodes a nucleotidyltransferase that triggers mRNA decapping ... to the poly(A) tail. As with Δ cutA , Δ edc3 led to reduced rates of transcript ...
Tópico(s): RNA and protein synthesis mechanisms
2010 - Wiley | Molecular Microbiology