... las becerras hasta unos 82 cm2 en los cutrales; en las pezuñas traseras desde unos 42 cm2 ... 600—700 kg) y sobre todo en los cutrales entre los pezuños posteriores laterales y mediales — los ... lactación, así como en 15 erales y 12 cutrales. Al progresar la gestación, el centro de gravedad ... la superficie total de las pezuñas, en los cutrales 56,6%. Citing Literature Volume15, Issue9November 1968Pages 844- ...
Tópico(s): Animal Nutrition and Physiology
2010 - Wiley | Zentralblatt für Veterinärmedizin Reihe A
Francesco Cutrale, Enrico Gratton,
Abstract Driven by the biological sciences, there is an increased need for imaging modalities capable of live cell imaging with high spatial and temporal resolution. To achieve this goal in a comprehensive manner, three‐dimensional acquisitions are necessary. Ideal features of a modern microscope system should include high imaging speed, high contrast ratio, low photo‐bleaching and photo‐toxicity, good resolution in a 3D context, and mosaic acquisition for large samples. Given the importance of ...
Tópico(s): Near-Field Optical Microscopy
2012 - Wiley | Microscopy Research and Technique
Produção Nacional
Revisado por pares
E. Haypek, Luiza Helena Meller da Silva, Eduardo Augusto Caldas Batista, Daniel S. Marques, M. Ângela A. Meireles, António J. A. Meirelles,
... the orange essential oil using experimental data from CUTRALE (a Brazilian Industry of Concentrated Orange Juice). The ...
Tópico(s): Advanced Chemical Sensor Technologies
2000 - Springer Science+Business Media | Brazilian Journal of Chemical Engineering
Francesco Cutrale, Anya Salih, Enrico Gratton,
The phasor global analysis algorithm is common for fluorescence lifetime applications, but has only been recently proposed for spectral analysis. Here the phasor representation and fingerprinting is exploited in its second harmonic to determine the number and spectra of photo-activated states as well as their conversion dynamics. We follow the sequence of photo-activation of proteins over time by rapidly collecting multiple spectral images. The phasor representation of the cumulative images provides ...
Tópico(s): Photosynthetic Processes and Mechanisms
2013 - IOP Publishing | Methods and Applications in Fluorescence
Francesco Cutrale, Vikas Trivedi, Le A. Trinh, Chi‐Li Chiu, John M. Choi, Marcela S Artiga, Scott E. Fraser,
Tópico(s): Zebrafish Biomedical Research Applications
2017 - Nature Portfolio | Nature Methods
Verónica Hortigüela, Enara Larrañaga, Francesco Cutrale, Anna Seriola, María García-Díaz, Anna Lagunas, Jordi Andilla, Pablo Loza‐Álvarez, Josep Samitier, Samuel Ojosnegros, Elena Martínez,
Here we present a nanostructured surface able to produce multivalent interactions between surface-bound ephrinB1 ligands and membrane EphB2 receptors. We created ephrinB1 nanopatterns of regular size (<30 nm in diameter) by using self-assembled diblock copolymers. Next, we used a statistically enhanced version of the Number and Brightness technique, which can discriminate—with molecular sensitivity—the oligomeric states of diffusive species to quantitatively track the EphB2 receptor oligomerization ...
Tópico(s): Chemical Synthesis and Analysis
2017 - American Chemical Society | Nano Letters
Produção Nacional
Revisado por pares
Samuel Ojosnegros, Francesco Cutrale, Daniel Rodríguez, Jason Otterstrom, Chi Li Chiu, Verónica Hortigüela, Carolina Tarantino, Anna Seriola, Stephen Mieruszynski, Elena Martínez, Melike Lakadamyali, Ángel Raya, Scott E. Fraser,
Significance Cell communication is a precisely orchestrated mechanism in which cell receptors translate extracellular cues into intracellular signals. The Eph receptors act as a model guidance system steering cells to defined positions by their ligand ephrin. However, we still lack a mechanistic understanding of how membrane receptors can read a wide range of ligand concentrations and gradients and integrate them into coherent cellular responses. Here we reveal the evolution of Eph aggregation upon ...
Tópico(s): Hippo pathway signaling and YAP/TAZ
2017 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Pu Wang, Gianluca Turcatel, Cosimo Arnesano, David Warburton, Scott E. Fraser, Francesco Cutrale,
Hyperspectral endoscopic imaging has the potential to enhance clinical diagnostics and outcome.Most commercial endoscopes utilize imaging fiber bundles to transmit the collected signal from the patient to the medical operator.These bundles consist of several fiber cores surrounded by a cladding layer creating comb structure-like artifacts, which complicate further analysis, both spatially and spectrally.Here we present an optical fiber pattern removal algorithm which we applied to hyperspectral ...
Tópico(s): Ocean Waves and Remote Sensing
2018 - Optica Publishing Group | Biomedical Optics Express
Produção Nacional
Revisado por pares
Francesco Cutrale, Daniel Rodríguez, Verónica Hortigüela, Chi‐Li Chiu, Jason Otterstrom, Stephen Mieruszynski, Anna Seriola, Enara Larrañaga, Ángel Raya, Melike Lakadamyali, Scott E. Fraser, Elena Martínez, Samuel Ojosnegros,
Tópico(s): Biotin and Related Studies
2019 - Nature Portfolio | Nature Protocols
Wen Shi, Daniel E. S. Koo, Masahiro Kitano, Hsiao Ju Chiang, Le A. Trinh, Gianluca Turcatel, Benjamin Steventon, Cosimo Arnesano, David Warburton, Scott E. Fraser, Francesco Cutrale,
Abstract Hyperspectral fluorescence imaging is gaining popularity for it enables multiplexing of spatio-temporal dynamics across scales for molecules, cells and tissues with multiple fluorescent labels. This is made possible by adding the dimension of wavelength to the dataset. The resulting datasets are high in information density and often require lengthy analyses to separate the overlapping fluorescent spectra. Understanding and visualizing these large multi-dimensional datasets during acquisition ...
Tópico(s): Cell Image Analysis Techniques
2020 - Nature Portfolio | Nature Communications
Athanasios Litsios, Pooja Goswami, Hanna M. Terpstra, Carleton H. Coffin, Luc-Alban Vuillemenot, Mattia Rovetta, Ghada Ghazal, Paolo Guerra, Katarzyna Buczak, Alexander Schmidt, Sylvain Tollis, Mike Tyers, Catherine A. Royer, Andreas Milias‐Argeitis, Matthias Heinemann,
... Moutin et al., 2014; Bourges et al., 2017; Cutrale et al., 2019; Zamai et al., 2019). 2psN& ...
Tópico(s): RNA and protein synthesis mechanisms
2022 - American Society for Cell Biology | Molecular Biology of the Cell
Hsiao Ju Chiang, Daniel E. S. Koo, Masahiro Kitano, Sean Burkitt, Jay R. Unruh, Cristina Zavaleta, Le A. Trinh, Scott E. Fraser, Francesco Cutrale,
Abstract The expansion of fluorescence bioimaging toward more complex systems and geometries requires analytical tools capable of spanning widely varying timescales and length scales, cleanly separating multiple fluorescent labels and distinguishing these labels from background autofluorescence. Here we meet these challenging objectives for multispectral fluorescence microscopy, combining hyperspectral phasors and linear unmixing to create Hybrid Unmixing (HyU). HyU is efficient and robust, capable ...
Tópico(s): Single-cell and spatial transcriptomics
2023 - Nature Portfolio | Nature Methods
Francesco Cutrale, Scott E. Fraser, Le A. Trinh,
Embryonic development is highly complex and dynamic, requiring the coordination of numerous molecular and cellular events at precise times and places. Advances in imaging technology have made it possible to follow developmental processes at cellular, tissue, and organ levels over time as they take place in the intact embryo. Parallel innovations of in vivo probes permit imaging to report on molecular, physiological, and anatomical events of embryogenesis, but the resulting multidimensional data ...
Tópico(s): Single-cell and spatial transcriptomics
2019 - Annual Reviews | Annual Review of Biomedical Data Science
Chi‐Li Chiu, Katherin Patsch, Francesco Cutrale, Anjana Soundararajan, David B. Agus, Scott E. Fraser, Daniel Ruderman,
Abstract The androgen receptor (AR) pathway plays a central role in prostate cancer (PCa) growth and progression and is a validated therapeutic target. In response to ligand binding AR translocates to the nucleus, though the molecular mechanism is not well understood. We therefore developed multimodal Image Correlation Spectroscopy (mICS) to measure anisotropic molecular motion across a live cell. We applied mICS to AR translocation dynamics to reveal its multimodal motion. By integrating fluorescence ...
Tópico(s): Prostate Cancer Treatment and Research
2016 - Nature Portfolio | Scientific Reports
Pu Wang, Masahiro Kitano, Kevin Keomanee-Dizon, Thai V. Truong, Scott E. Fraser, Francesco Cutrale,
Hyperspectral fluorescence imaging improves multiplexed observations of biological samples by utilizing multiple color channels across the spectral range to compensate for spectral overlap between labels. Typically, spectral resolution comes at a cost of decreased detection efficiency, which both hampers imaging speed and increases photo-toxicity to the samples. Here, we present a high-speed, high-efficiency snapshot spectral acquisition method, based on optical compression of the fluorescence spectra ...
Tópico(s): Optical Coherence Tomography Applications
2023 - Elsevier BV | Cell Reports Methods
Albert Parra, Denitza Denkova, Xavier P. Burgos-Artizzu, Ester Aroca, Marc Casals, Amélie Luise Godeau, Miguel Ares, Anna Ferrer‐Vaquer, Ot Massafret, Irene Oliver‐Vila, Enric Mestres, Mònica Acacio, Nuno Costa-Borges, Elena Rebollo, Hsiao Ju Chiang, Scott E. Fraser, Francesco Cutrale, Anna Seriola, Samuel Ojosnegros,
Only 30% of embryos from in vitro fertilized oocytes successfully implant and develop to term, leading to repeated transfer cycles. To reduce time-to-pregnancy and stress for patients, there is a need for a diagnostic tool to better select embryos and oocytes based on their physiology. The current standard employs brightfield imaging, which provides limited physiological information. Here, we introduce METAPHOR: Metabolic Evaluation through Phasor-based Hyperspectral Imaging and Organelle Recognition. ...
Tópico(s): Molecular Biology Techniques and Applications
2024 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Sara Caviglia, Iris A. Unterweger, Akvilė Gasiūnaitė, Alexandre E. Vanoosthuyse, Francesco Cutrale, Le A. Trinh, Scott E. Fraser, Stephan C. F. Neuhauss, Elke A. Ober,
Visualizing cell shapes and interactions of differentiating cells is instrumental for understanding organ development and repair. Across species, strategies for stochastic multicolour labelling have greatly facilitated in vivo cell tracking and mapping neuronal connectivity. Yet integrating multi-fluorophore information into the context of developing zebrafish tissues is challenging given their cytoplasmic localization and spectral incompatibility with common fluorescent markers. Inspired by Drosophila ...
Tópico(s): Single-cell and spatial transcriptomics
2022 - The Company of Biologists | Development
Produção Nacional
Revisado por pares
Márcio Roberto Toledo, Ricardo Castillo,
... article is to understand how the juice tradings (Cutrale, Citrosuco, Citrovita, Coinbra/Dreyfuss and Cargill) make a ...
Tópico(s): Urban Development and Societal Issues
2008 - UNIVERSIDADE FEDERAL DE SANTA CATARINA | Geosul
Thai V. Truong, Vikas Trivedi, Le A. Trinh, Daniel B. Holland, Francesco Cutrale, John M. Choi, Scott E. Fraser,
The developing vertebrate heart is a highly dynamic organ that starts to function early on during embryonic development, even as it continues to undergo dramatic morphological changes and cellular differentiation. Fast and high resolution three-dimensional (3D) imaging is needed to document the intrinsic cellular dynamics of the beating heart, as a critical step in understanding its development. To meet the challenges of obtaining sub-cellular resolution imaging of a dynamic 100-micron length scale ...
Tópico(s): Advanced Electron Microscopy Techniques and Applications
2014 - Elsevier BV | Biophysical Journal
Shanshan Cai, D. John, Winn Hong, Scott E. Fraser, Francesco Cutrale,
Tópico(s): Infrared Thermography in Medicine
2025 - Cell Press | iScience
Photo-Activated Localization Microscopy (P.A.L.M.) as described by E. Betzig (2006) optically resolves selected subsets of photo-activatable fluorescent probes within cells at mean separations of less than 25 nanometers through serial photo-activation and subsequent photobleaching of numerous sparse subsets of photo-activated fluorescent protein molecules. The position information from all subsets is then assembled into a super-resolution image, in which individual fluorescent molecules are isolated ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2009 - Elsevier BV | Biophysical Journal
Francesco Cutrale, Enrico Gratton,
Analysis of the fluctuations in time and space of confocal images has the potential to provide information about molecular diffusion and molecular interactions directly in live cells. Fluctuation image analysis has been commonly done in the laser scanning microscope. In the slow regime, when the fluctuations are slower than the frame rate, the time correlation between the same pixel in different frames of an image stack provides all the information about diffusion and brightness. In the fast regime, ...
Tópico(s): nanoparticles nucleation surface interactions
2010 - Elsevier BV | Biophysical Journal
Francesco Cutrale, Enrico Gratton,
Current need in biomedical sciences requests live imaging with higher spatial and temporal resolution in the 3D environment. Furthermore, live sample acquisition increases the technical challenges required for 3D imaging. Ideal features of a system for this purpose include high imaging speed, high contrast ratio, low photo-bleaching and photo-toxicity, good resolution in a 3D context and mosaic acquisition for large samples. Our work presents the inclined illumination implementation of Single Plane ...
Tópico(s): Advanced Electron Microscopy Techniques and Applications
2012 - Elsevier BV | Biophysical Journal
Francesco Cutrale, Vikas Trivedi, Le A. Trinh, Thai V. Truong, Scott E. Fraser,
Hyperspectral imaging provides the potential for assessing biochemical interactions in the zebrafish embryo in a label-free manner that extends beyond conventional morphological and molecular phenotyping. It takes advantage of the intrinsic wavelengths emitted or reflected from a sample without the need for extrinsic staining methods. The specific spectral signature from a sample can arise from chemical interactions, molecular bonds and macro-structural arrangements. A challenge in hyperspectral ...
Tópico(s): Spectroscopy Techniques in Biomedical and Chemical Research
2014 - Elsevier BV | Biophysical Journal
Le A. Trinh, Francesco Cutrale, Scott E. Fraser, Jim P. Kilcrease, Eduardo Rosa‐Molinar,
In recent years, light microscopy technology has pushed the envelope to achieve higher temporal, spatial, and spectral resolution [1][2][3] .These advance techniques have enabled fluorescence microscopy to provide qualitative and quantitative readouts of cellular and molecular phenotypes in fixed and live samples and has transformed cell and molecular data 4, 5 .However, efforts to achieve higher resolution in optical microscopy and realize its full potential are hampered by the obstacle presented ...
Tópico(s): Force Microscopy Techniques and Applications
2017 - Oxford University Press | Microscopy and Microanalysis
RESUMEN: En el escrito, después de una introducción general para comprender la problemática del mundo islámico y aclarar algunas definiciones, se lleva a cabo una investigación sobre el fenómeno del terrorismo yihadista, empezando con un breve excursus sobre el debate en torno a la definición de terrorismo internacional. Posteriormente, se analiza la estrutura del Estado islámico y cómo ha evolucionado durante la ultima década, el concepto de radicalización y, considerando diferentes estudios y ...
Tópico(s): Terrorism, Counterterrorism, and Political Violence
2019 - | UNIVERSITAS Revista de Filosofía Derecho y Política
RESUMEN: La agresión terrorista ha supuesto, a nivel nacional y internacional, el problema de la creación de un sistema penal de reacción a tal fenómeno. Sin embargo, la necesidad de una contestación urgente ha tenido como consecuencia una incertidumbre general que lleva en sí misma el riesgo de la negación del Estado de derecho para adoptar la lógica del derecho penal del enemigo. Este escrito -a través el análisis de la teoría de Gunther Jakobs, teórico del derecho penal del enemigo- describe las ...
Tópico(s): Social Issues and Policies in Latin America
2019 - | UNIVERSITAS Revista de Filosofía Derecho y Política
Samuel Ojosnegros, Andrés Parra, Denitza Denkova, Xavier P. Paolo Burgos-Artizzu, I.T. Oliver, Scott E. Fraser, Hsin‐Ju Chiang, Francesco Cutrale, Nuno Costa-Borges, Enric Mestres, Mònica Acacio, Gloria Calderón, Elena Rebollo, Anna Veiga, Anna Seriola,
Abstract Study question To classify embryos according to their metabolic fingerprint with a robust, non-invasive methodology Summary answer Our Hyperspectral (HS) phasor analysis coupled to artificial intelligence simultaneously detects 6+ metabolites to profile and classify competent embryos and oocytes safely and consistently. What is known already Metabolism plays a key role in oocyte and embryo developmental competence. However, current methods to measure oocyte and embryo metabolism are either ...
Tópico(s): Animal Genetics and Reproduction
2023 - Oxford University Press | Human Reproduction
Rafael Tedim Terra, Alex Cardoso dos Santos, Vitor Cutrale,
Tópico(s): Industrial Technology and Control Systems
2021 - | SAE technical papers on CD-ROM/SAE technical paper series
Pu Wang, Masahiro Kitano, Kevin Keomanee-Dizon, Thai V. Truong, Scott E. Fraser, Francesco Cutrale,
Hyperspectral fluorescence imaging improves multiplexed observations of biological samples by utilizing multiple color channels across the spectral range to compensate for spectral overlap between labels. Typically, spectral resolution comes at a cost of decreased detection efficiency, which both hampers imaging speed and increases photo-toxicity to the samples. Here, we present a high-speed, high-efficiency snapshot spectral acquisition method, based on optical compression of the fluorescence spectra ...
Tópico(s): Nanoplatforms for cancer theranostics
2022 - RELX Group (Netherlands) | SSRN Electronic Journal