... weeks could not produce significant delayed-type hypersensitivity (DTH) reactions to the specific erythrocyte used for feeding. Diminished DTH reactions persisted for at least 6 months after feeding was stopped. Although DTH was diminished in vivo, cells sensitized for DTH as well as suppressor cells could be detected ... mice. The presence of spleen cells sensitized for DTH was revealed by the transfer of spleen cells ... spleen cells from SRBC-fed to normal mice. DTH reactions were not suppressed by the transfer of ...
Tópico(s): Food Allergy and Anaphylaxis Research
1978 - American Association of Immunologists | The Journal of Immunology
Alexander Street
Gideon Strassmann, Zelig Eshhar, Edna Mozes,
Delayed-type hypersensitivity (DTH) responses served in this study as an experimental model for the analysis of genetic regulations of T-cell responses. ... F1 hybrids between high and low responders mediated DTH responses, which indicates that the gene(s) controlling the DTH responses is dominant. To analyze the genetic defect in DTH responses to (T,G)-A--L, we separated ... between the parental (nonresponder x responder) F1 recipients, DTH responses could have been manifested. (b) On the other hand, no DTH responses could be mediated by transferring educated cells ...
Tópico(s): Viral Infectious Diseases and Gene Expression in Insects
1980 - Rockefeller University Press | The Journal of Experimental Medicine
... induce a persistent state of delayed type hypersensitivity (DTH) to minor histocompatibility (H) antigens which can be ... the recipient for (1) the elicitation of the DTH‐reaction, (2) the induction of DTH‐related effector and memory T cells and (3) ... 2 complex with the recipient could elicit strong DTH‐reactions to minor H antigens, though somewhat less ... region coded antigens elicited relatively weak, though significant, DTH‐reactivity to the minor H antigens. Similar H‐ ... immune lymphocyte transfer assay. Optimal induction of primary DTH and DTH‐related T memory cells for minor ...
Tópico(s): Immunotherapy and Immune Responses
1980 - Wiley | International Journal of Immunogenetics
Theodorus van der Kwast, Johanna G. Olthof, H. de Ruiter, Robbert Benner,
Secondary delayed type hypersensitivity (DTH) to sheep red blood cells (SRBC) in mice is a long-lived memory phenomenon which is characterized by the accelerated reappearance of the state of DTH after a booster injection of the antigen. In this paper the nature of the DTH-related T memory cells accounting for secondary DTH was investigated. Parabiosis of primed and nonprimed mice ... 4 weeks resulted in an equally large secondary DTH responsiveness in both partners. This ability was maintained ... of the parabiosis. These results indicate that (a) DTH-related T memory cells are potentially circulating cells, ...
Tópico(s): Asthma and respiratory diseases
1979 - Elsevier BV | Cellular Immunology
Foo Y. Liew, Stuart Russell, Colin M. Brand,
... virus or viral subunits developed delayed‐type hypersensitivity (DTH) to influenza virus. The level of DTH was greatly enhanced when mice were injected intraperitoneally ... at 24 h after elicitation, had the classical DTH histology, and was transferable by immune cells but not by immune serum. DTH induced in cyclophosphamide‐pretreated mice persisted for at least 40 days after sensitization. DTH induced by deoxycholate‐treated subviral particles (DC particles) ... the influenza virus was type‐specific, i.e. DTH induced by DC particles or matrix protein of ...
Tópico(s): interferon and immune responses
1979 - Wiley | European Journal of Immunology
E. A. J. Wolters, Robbert Benner,
... associated with a specific antihost delayed-type hypersensitivity (DTH) in the lymphoid organs of these mice. In ... role of proliferation in the development of this DTH response was studied in relation to the cellular ... the irradiated recipients did not prevent the antihost DTH responsiveness in the spleen during the first 2 ... nodes at 3 days after reconstitution. Thereafter, the DTH responsiveness was greatly decreased by the mitomycin C ... per ml could completely prevent the development of DTH responsiveness in the recipient mice. Elimination of the ...
Tópico(s): T-cell and B-cell Immunology
1979 - Wolters Kluwer | Transplantation
Shinichi Tamura, Asato Kojima, Yasuyuki Egashira,
The memory of delayed-type hypersensitivity (DTH), manifested by the augmented responsiveness upon challenge with alum-absorbed ovalbumin (OA), was induced in mice primed 7 days, 21 days, or ... The memory cells involved in the augmentation of DTH responses were analyzed in the in vitro induction system of T cells which mediate DTH against OA. Spleen cells from the primed mice generated DTH-effector T cells (DTH-Te) in a significantly accelerated fashion, compared with ... when cultured with OA. The accelerated generation of DTH-Te in vitro was induced antigen specifically and ...
Tópico(s): Asthma and respiratory diseases
1981 - Wiley | Microbiology and Immunology
Shinichi Tamura, Yoko Nakanishi, Asato Kojima, Minoru Otokawa, Nobuyuki Uchida, Hiroko Sato, Yuji Sato,
... of pertussis toxin (PT) on delayed-type hypersensitivity (DTH) to ovalbumin (OA) and on the activity of suppressor T cells on the DTH (DTH-Ts). Mice immunized with alum-precipitated ovalbumin showed a transient DTH, which was determined as footpad swelling which disappeared ... Maximal footpad swelling was observed 24 hr after DTH elicitation. On the other hand, when mice received ... mouse) at the time of immunization, the transient DTH became an enhanced and persistent DTH, which persisted for at least 4 weeks. In ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
1983 - Elsevier BV | Cellular Immunology
Philip W. Askenase, Henk Van Loveren, Sandra Kraeuter‐Kops, Yacov Ron, Robin Meade, T.C. Theoharides, James J. Nordlund, H Scovern, M D Gerhson, W Ptak,
... involved in the elicitation of delayed-type hypersensitivity (DTH) in mice. Mast cells are thought to be required in DTH to release serotonin to open gaps between endothelial ... controls, were studied for their ability to express DTH. Both strains were shown to be deficient in ... cells at skin sites of preferential elicitation of DTH in normal mice, such as the ear or footpad. Defective DTH was found in both mast cell-deficient strains ... two different systems: 1) sheep erythrocyte-induced footpad DTH, and 2) picryl chloride-induced contact sensitivity ear ...
Tópico(s): Asthma and respiratory diseases
1983 - American Association of Immunologists | The Journal of Immunology
Geneviève Milon, G Marchal, Michel Séman, Paolo Truffa‐Bachi, Vladimir Zilberfarb,
... optimal for antibody response, no delayed-type hypersensitivity (DTH) reaction is detectable. In contrast, in mice receiving ... too small to induce B cell activation, a DTH reaction is elicitable shortly and transiently after immunization. Using a sensitive titration assay of DTH-mediating T lymphocytes, this reciprocal relationship between antibody production and DTH responses was reinvestigated. The absence of peripheral DTH reactivity in mice primed i.v. with a ... the absence of activation and clonal expansion of DTH-mediating cells or from induction of suppressive mechanisms ...
Tópico(s): Asthma and respiratory diseases
1983 - American Association of Immunologists | The Journal of Immunology
Shinichi Tamura, Yoko Nakanishi, Asato Kojima, Minoru Otokawa, Nobuyuki Uchida, Hiroko Sato, Yuji Sato,
... sensitized T-cell populations for delayed-type hypersensitivity (DTH) were examined in mice. DTH was induced by sensitizing mice with ovalbumin (OA) and elicited by injecting OA into the footpad. DTH could be conferred on naive recipient mice by ... mice were given PT at the time of DTH-elicitation, they did not express a high DTH reaction, with the maximum reaction 24 hr after ... just before intravenous injection of sensitized spleen cells, DTH was not conferred. In addition, when the sensitized ... with PT in vitro and then transferred intravenously, DTH was not conferred in recipient mice. However, DTH ...
Tópico(s): Asthma and respiratory diseases
1984 - Elsevier BV | Cellular Immunology
Herman Bril, Th. W. van den Akker, B.D. Molendijk-Lok, A.T.J. Bianchi, R. Benner,
... allogeneic spleen cells can induce delayed-type hypersensitivity (DTH) to both major and minor histocompatibility antigens. Intravenous ... spleen cells, however, induces a poor state of DTH. Furthermore, i.v. immunization with allogeneic spleen cells, ... capable of suppressing the host-vs-graft (HvG) DTH reactivity that normally arises after s.c. immunization. ... they can suppress the development of anti-host DTH effector T cells during graft-vs-host (GvH) reactions. These models for HvG and GvH DTH reactivity were used to study the influence of ...
Tópico(s): Cytomegalovirus and herpesvirus research
1984 - American Association of Immunologists | The Journal of Immunology
Yasuyuki Takai, Atsushi Kosugi, Takayuki Yoshioka, S Tomita, H Fujiwara, Takafumi Hamaoka,
... the in vivo development of delayed-type hypersensitivity (DTH) responses was investigated. C3H/HeN mice were inoculated ... later, these mice were tested for anti-TNP DTH responses either by challenging them directly with TNP- ... primed mice failed to generate significant anti-TNP DTH responses when s.c. immunization was provided by ... not unprimed mice, could generate augmented anti-TNP DTH responses when immunized with virus-self-TNP. Anti- ... mediated by Thy-1+, Lyt-1+2- cells. DTH effector cells enhanced by helper T cells were ...
Tópico(s): Immunotherapy and Immune Responses
1985 - American Association of Immunologists | The Journal of Immunology
Shinichi Tamura, Hiroshi Tanaka, Ritsuko Takayama, Hiroko Sato, Yuji Sato, Nobuyuki Uchida,
... PT) on the unresponsiveness of delayed-type hypersensitivity (DTH) to sheep red blood cells (SRBC) were examined in mice. DTH cannot be detected in mice injected iv with ... replaced by the development of high and persistent DTH responses with several days' lag after PT treatment. The restored DTH response was accompanied by the appearance of circulating DTH-mediating cells. In the non-PT-treated mice, DTH-mediating cells, which conferred DTH in syngeneic naive recipient mice by systemic transfer, ... function of splenic suppressor T cells (Ts) for DTH in mice injected iv with 109 SRBC was ...
Tópico(s): T-cell and B-cell Immunology
1985 - Elsevier BV | Cellular Immunology
... was conducted to evaluate the delayed-type hypersensitivity (DTH) test in diagnosing brucellosis in cattle, in particular ... milk ring test (MRT) were subjected to the DTH test. The cows were then slaughtered and the ... collected for bacteriologic examination. In 989 cows the DTH test, MRT and serologic tests were negative. When the DTH test results were compared with bacteriologic results, 12 ... CFT titres >1:200 tested negative in the DTH test while bacteriologic results were positive. The sensitivity of the DTH test (calculated on the remaining 81 cows) was ...
Tópico(s): Vector-Borne Animal Diseases
1990 - Elsevier BV | Veterinary Microbiology
W R Herzog, Isabelle Millet, Nicholas R. Ferreri, Rajani Ramabhadran, Jolanda Schreurs, Philip W. Askenase,
The elicitation of delayed-type hypersensitivity (DTH) reactions in mice is due to the sequential action of two different Ag-specific Thy-1+ cells. An early-acting DTH-initiating cell in the lymphoid organs produces a ... is functionally analogous to IgE antibody and initiates DTH by sensitizing the local tissue for release of ... chloride (PC1) or oxazolone (OX) contact sensitivity, this DTH-initiating factor is called PC1-F and OX- ... specific, but MHC-unrestricted. The phenotype of polyclonal DTH-initiating cells was recently shown to be unusual ...
Tópico(s): Fluorine in Organic Chemistry
1990 - American Association of Immunologists | The Journal of Immunology
Martyn A. French, Paul Cameron, G. Grimsley, L.A. Smyth, R.L. Dawkins,
... patients had persistent depression of delayed-type hypersensitivity (DTH) responses prior to commencing therapy. In 11 of ... 19 patients (58%) there was sustained improvement of DTH responses with the maximal effect occurring at approximately 3 months after therapy was started. DTH declined after 3 months but remained significantly higher ... who did not have a sustained increase in DTH responses had more severe disease than those that ... patients on zidovudine therapy, but varied independently of DTH responses. Epidermal Langerhans cell density was lower in ...
Tópico(s): Drug-Induced Adverse Reactions
1990 - Academic Press | Clinical Immunology and Immunopathology
C M Lawson, J. E. Grundy, G. R. Shellam,
Summary The delayed-type hypersensitivity (DTH) response in mice infected with murine cytomegalovirus (MCMV) was measured by ear swelling following a challenge with heat-treated MCMV. DTH was dose-dependent and could be detected as ... occurring between days 15 and 21 postinfection. The DTH response was found to be specific for MCMV ... A virus in MCMV-primed mice. The elicited DTH response was greater in mice primed with attenuated compared with virulent MCMV. The DTH response was shown to correlate positively with the ...
Tópico(s): Tryptophan and brain disorders
1987 - Microbiology Society | Journal of General Virology
W Ptak, Douglas R. Green, Patrick M. Flood,
The adoptive transfer of delayed-type hypersensitivity (DTH) into naive recipients requires the interaction of two functionally distinct Ly-1+ T cells: and I-J- cell effector cell for DTH which transfers antigen-specific DTH only into animals whose suppressive mechanisms have been ... and I-J+ cell which alone never transfers DTH but allows the transfer of DTH by the I-J- DTH effector cell into naive animals. We investigated the ... of the cell which "protects" the I-J- DTH effector cell from host suppressive mechanisms and allows ...
Tópico(s): Bacteriophages and microbial interactions
1986 - American Association of Immunologists | The Journal of Immunology
A T Bianchi, L.M. Hussaarts-Odijk, E. A. J. Wolters, Arthur J. Molendijk, R. Benner,
... Ts) cells that can suppress delayed type hypersensitivity (DTH) against histocompatibility (H) antigens can be isolated from ... investigated the suppression of the efferent phase of DTH to characterize the Ts cells involved, and to ... in a previous paper of this series. The DTH against third party alloantigens that were not used ... alloantigens in the challenge inoculum for eliciting the DTH reaction. Thus the ultimate suppressive effect by the ... that are active during the efferent phase of DTH is nonspecific. This non-specific suppression of DTH ...
Tópico(s): Immunotherapy and Immune Responses
1986 - American Association of Immunologists | The Journal of Immunology
Loran M. Killar, Toby K. Eisenstein,
... correlate immunity and expression of delayed-type hypersensitivity (DTH) in mice of the C3H lineage immunized with ... SL3235 on the development of protective immunity and DTH was examined. It was found that in hypersusceptible ... mice of these two strains never mounted significant DTH responses following immunization with the doses of strain ... colonized with strain SL3235 at the time of DTH elicitation. Further, it was found that the ability of responsive C3H/HeNCrlBR mice to display DTH was dependent on the immunizing dose of strain ...
Tópico(s): Escherichia coli research studies
1986 - American Society for Microbiology | Infection and Immunity
Richard C. Weiss, Nancy R. Cox,
Delayed-type hypersensitivity (DTH)-like reactions to feline infectious peritonitis (FIP) virus (FIPV) were induced in the skin of nine cats that were ... at the time of intradermal (ID) testing for DTH. Two other cats tested for DTH when acutely ill with clinical FIP did not have cutaneous DTH responses to FIPV. Gross skin reactions to FIPV ... or semi-firm, nonerythematous, slightly raised nodules. Microscopically, DTH-like reactions were observed in biopsies taken from ... 72. Five of nine cats (56%) with positive DTH skin responses had increased survival times after lethal ...
Tópico(s): Animal Virus Infections Studies
1989 - Elsevier BV | Veterinary Immunology and Immunopathology
W R Herzog, Nicholas R. Ferreri, W Ptak, Philip W. Askenase,
Abstract The elicitation of delayed-type hypersensitivity (DTH) requires an early-acting Thy-1+ cell that produces an Ag-specific, non-MHC-restricted factor that initiates DTH by sensitizing the local tissue for release of ... amine serotonin. We characterized the phenotype of this DTH-initiating cell by treating cells from sensitized mice ... into naive recipients that were challenged to elicit DTH. Our findings indicate that the early DTH-initiating cell is Thy-1+, Lyt-1+, CD4-, CD8- and CD3-, whereas the classical, late DTH effector T cell is Thy-1+, Lyt-1+, ...
Tópico(s): Immune Cell Function and Interaction
1989 - American Association of Immunologists | The Journal of Immunology
Jean‐Claude Ameisen, Robert C. Meade, P W Askenase,
... for 5-HT has been proposed in murine DTH and has been attributed to its 5-HT2R- ... hypothesis that the role of 5-HT in DTH is related to an interaction of 5-HT with DTH effector T cells. In vivo treatment of sensitized ... or ketanserin inhibited both their capacity to elicit DTH and the ability of their lymphoid cells to transfer DTH. In vitro treatment of lymphoid cells, or of ... washings, inhibited as strongly their ability to transfer DTH, both systemically or locally. Systemic and local co- ... the efferent, but not the afferent limb of DTH; and in the efferent T cell cascade, affected ...
Tópico(s): Synthesis and Reactions of Organic Compounds
1989 - American Association of Immunologists | The Journal of Immunology
W R Herzog, Robert D. Meade, A Pettinicchi, W Ptak, Philip W. Askenase,
The elicitation of delayed-type hypersensitivity (DTH) reactions in mice is caused by the sequential action of two different T cells. An early-acting, DTH-initiating T cell produces an Ag-specific T ... that is analogous to IgE antibody and initiates DTH by sensitizing the local tissues for release of ... specific, but MHC unrestricted. We, therefore, hypothesized that DTH-initiating T cells are primitive T cells with ... In order to characterize the origin of this DTH-initiating T cell and the conditions that are ...
Tópico(s): Immune Cell Function and Interaction
1989 - American Association of Immunologists | The Journal of Immunology
Robert L. Hendricks, Meiyi Tao, Joseph C. Glorioso,
... that extended to both the delayed type hypersensitivity (DTH) and CTL responses. A mutant strain lacking gC ( ... characterized by undetectable CTL activity but a normal DTH response. A mutant strain lacking gB (gB-) caused ... the CTL response and no reduction of the DTH response. Thus, whereas gB may be involved in ... be present in the AC to induce detectable DTH tolerance. The latter interpretation was strengthened by the ... and gB- (expressing normal gC) effectively suppressed the DTH response to wild-type HSV-1. A panel ...
Tópico(s): Immunotherapy and Immune Responses
1989 - American Association of Immunologists | The Journal of Immunology