Ruben K. Dagda, Julie A. Zaucha, Brian E. Wadzinski, Stefan Strack,
... β-propeller (15Strack S. Ruediger R. Walter G. Dagda R.K. Barwacz C.A. Cribbs J.T. ... described previously (15Strack S. Ruediger R. Walter G. Dagda R.K. Barwacz C.A. Cribbs J.T. ... as described (15Strack S. Ruediger R. Walter G. Dagda R.K. Barwacz C.A. Cribbs J.T. ... Bγ subunit (15Strack S. Ruediger R. Walter G. Dagda R.K. Barwacz C.A. Cribbs J.T. ... C subunits (15Strack S. Ruediger R. Walter G. Dagda R.K. Barwacz C.A. Cribbs J.T. ...
Tópico(s): Bone Metabolism and Diseases
2003 - Elsevier BV | Journal of Biological Chemistry
Stefan Strack, J. Thomas Cribbs, Lisa L. Gomez,
... subcellular localization (7Strack S. Ruediger R. Walter G. Dagda R.K. Barwacz C.A. Cribbs J.T. ... proteosomal degradation (7Strack S. Ruediger R. Walter G. Dagda R.K. Barwacz C.A. Cribbs J.T. ... regulatory subunits (7Strack S. Ruediger R. Walter G. Dagda R.K. Barwacz C.A. Cribbs J.T. ...
Tópico(s): RNA and protein synthesis mechanisms
2004 - Elsevier BV | Journal of Biological Chemistry
Tania Das Banerjee, Raul Y. Dagda, Marisela Dagda, Charleen T. Chu, Monica Rice, Emmanuel Vazquez‐Mayorga, Ruben K. Dagda,
Mitochondrial Protein Kinase A (PKA) and PTEN-induced kinase 1 (PINK1), which is linked to Parkinson's disease, are two neuroprotective serine/threonine kinases that regulate dendrite remodeling and mitochondrial function. We have previously shown that PINK1 regulates dendrite morphology by enhancing PKA activity. Here, we show the molecular mechanisms by which PINK1 and PKA in the mitochondrion interact to regulate dendrite remodeling, mitochondrial morphology, content, and trafficking in dendrites. ...
Tópico(s): Parkinson's Disease Mechanisms and Treatments
2017 - Wiley | Journal of Neurochemistry
... G proteins (11Strack S. Ruediger R. Walter G. Dagda R.K. Barwacz C.A. Cribbs J.T. ... proteasome pathway (11Strack S. Ruediger R. Walter G. Dagda R.K. Barwacz C.A. Cribbs J.T. ...
Tópico(s): Nuclear Receptors and Signaling
2002 - Elsevier BV | Journal of Biological Chemistry
Ruben K. Dagda, Ronald A. Merrill, J. Thomas Cribbs, Yucui Chen, Johannes Hell, Yuriy M. Usachev, Stefan Strack,
... Google Scholar, 27Strack S. Ruediger R. Walter G. Dagda R.K. Barwacz C.A. Cribbs J.T. ...
Tópico(s): Genetic Neurodegenerative Diseases
2008 - Elsevier BV | Journal of Biological Chemistry
Anna-Kate Fowler, Aveline Hewetson, Rajiv Agrawal, Marisela Dagda, Raul Y. Dagda, Ruin Moaddel, Silvia Balbo, Mitesh Sanghvi, Yukun Chen, Ryan J. Hogue, Susan E. Bergeson, George I. Henderson, Inna I. Kruman,
The brain is one of the major targets of chronic alcohol abuse. Yet the fundamental mechanisms underlying alcohol-mediated brain damage remain unclear. The products of alcohol metabolism cause DNA damage, which in conditions of DNA repair dysfunction leads to genomic instability and neural death. We propose that one-carbon metabolism (OCM) impairment associated with long term chronic ethanol intake is a key factor in ethanol-induced neurotoxicity, because OCM provides cells with DNA precursors for ...
Tópico(s): DNA Repair Mechanisms
2012 - Elsevier BV | Journal of Biological Chemistry
Michael J. Van Kanegan, Deanna G. Adams, Brian E. Wadzinski, Stefan Strack,
... Google Scholar, 5Strack S. Ruediger R. Walter G. Dagda R.K. Barwacz C.A. Cribbs J.T. ... in cells (5Strack S. Ruediger R. Walter G. Dagda R.K. Barwacz C.A. Cribbs J.T. ...
Tópico(s): PI3K/AKT/mTOR signaling in cancer
2005 - Elsevier BV | Journal of Biological Chemistry
Ruben K. Dagda, Chris Barwacz, J. Thomas Cribbs, Stefan Strack,
... to mitochondria to promote apoptosis in PC12 cells (Dagda, R. K., Zaucha, J. A., Wadzinski, B. E., ...
Tópico(s): RNA and protein synthesis mechanisms
2005 - Elsevier BV | Journal of Biological Chemistry
Ruben K. Dagda, Irene Pien, Ruth Wang, Jianhui Zhu, Kent Z.Q. Wang, Jason Callio, Tania Das Banerjee, Raul Y. Dagda, Charleen T. Chu,
The subcellular compartmentalization of kinase activity allows for regulation of distinct cellular processes involved in cell differentiation or survival. The PTEN-induced kinase 1 (PINK1), which is linked to Parkinson's disease, is a neuroprotective kinase localized to cytosolic and mitochondrial compartments. While mitochondrial targeting of PINK1 is important for its activities regulating mitochondrial homeostasis, the physiological role of the cytosolic pool of PINK1 remains unknown. Here, we ...
Tópico(s): Retinal Development and Disorders
2013 - Wiley | Journal of Neurochemistry
Emmanuel Vazquez‐Mayorga, Ángel G. Díaz‐Sánchez, Ruben K. Dagda, Carlos Domínguez-Solís, Raul Y. Dagda, Cynthia K Coronado-Ramirez, Alejandro Martínez‐Martínez,
Mutations the in human DJ-1 (hDJ-1) gene are associated with early-onset autosomal recessive forms of Parkinson's disease (PD). hDJ-1/parkinsonism associated deglycase (PARK7) is a cytoprotective multi-functional protein that contains a conserved cysteine-protease domain. Given that cysteine-proteases can act on both amide and ester substrates, we surmised that hDJ-1 possessed cysteine-mediated esterase activity. To test this hypothesis, hDJ-1 was overexpressed, purified and tested for activity towards ...
Tópico(s): Free Radicals and Antioxidants
2016 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Wayne Hope, Alexei V. Tchesunov,
... COBB 1920 is synonymized with lonema COBB 1920. Dagda SOUTHERN 1914 and Diodontolaimus SOUTHERN 1914 are transferred to Camacolaiminae, although Dagda asymmetrica GERLACH 1953, D. phinneyi MURPHY 1964, and ...
Tópico(s): Nematode management and characterization studies
1999 - Wiley | Invertebrate Biology
Juliana L. Sacoman, Raul Y. Dagda, Amanda R. Burnham‐Marusich, Ruben K. Dagda, Patricia Berninsone,
O-Linked N-acetylglucosamine transferase (OGT) catalyzes O-GlcNAcylation of target proteins and regulates numerous biological processes. OGT is encoded by a single gene that yields nucleocytosolic and mitochondrial isoforms. To date, the role of the mitochondrial isoform of OGT (mOGT) remains largely unknown. Using high throughput proteomics, we identified 84 candidate mitochondrial glycoproteins, of which 44 are novel. Notably, two of the candidate glycoproteins identified (cytochrome oxidase 2 (COX2) ...
Tópico(s): Carbohydrate Chemistry and Synthesis
2017 - Elsevier BV | Journal of Biological Chemistry
Mariana Grigoruţă, Alejandro Martínez‐Martínez, Raul Y. Dagda, Ruben K. Dagda,
Psychological distress is a public health issue as it contributes to the development of human diseases including neuropathologies. Parkinson's disease (PD), a chronic, progressive neurodegenerative disorder, is caused by multiple factors including aging, mitochondrial dysfunction, and/or stressors. In PD, a substantial loss of substantia nigra (SN) neurons leads to rigid tremors, bradykinesia, and chronic fatigue. Several studies have reported that the hypothalamic-pituitary-adrenal (HPA) axis is altered ...
Tópico(s): Parkinson's Disease Mechanisms and Treatments
2019 - Springer Science+Business Media | Molecular Neurobiology
Smijin Soman, David Tingle, Raul Y. Dagda, Mariana Torres, Marisela Dagda, Ruben K. Dagda,
Mutations in PTEN-induced kinase 1 (PINK1) lead to early onset autosomal recessive Parkinson's disease in humans. In healthy neurons, full-length PINK1 (fPINK1) is post-translationally cleaved into different lower molecular weight forms, and cleaved PINK1 (cPINK1) gets shuttled to the cytosolic compartments to support extra-mitochondrial functions. While numerous studies have exemplified the role of mitochondrially localized PINK1 in modulating mitophagy in oxidatively stressed neurons, little is known ...
Tópico(s): Autophagy in Disease and Therapy
2021 - Wiley | Journal of Neuroscience Research
... Lujan, Christopher Kushmerick, Tania Das Banerjee, Ruben K. Dagda, and Robert Renden1 December 2016 | Journal of Neurophysiology, ...
Tópico(s): Connexins and lens biology
1963 - American Physiological Society | Journal of Neurophysiology
Helen E. Moritz, Donna Rosenberg,
... Isles Ireland/Scotland The Ages of the World Dagda the Good England/Scandinavia Beowulf England/France King ...
Tópico(s): Folklore, Mythology, and Literature Studies
1987 - Wiley | Modern Language Journal
Ruben K. Dagda, Charleen T. Chu,
Alterations in mitochondrial homeostasis have been implicated in the etiology of Parkinson disease (PD) as demonstrated by human tissue studies, cell culture and in vivo genetic and toxin models. Mutations in the genes encoding PTEN-induced kinase 1 (PINK1), Omi/HtrA2 and parkin contribute to rare forms of parkinsonian neurodegeneration. Recently, each of these proteins has been shown to play a normal role in regulating mitochondrial structure, function, fission-fusion dynamics, or turnover (autophagy ...
Tópico(s): Amyotrophic Lateral Sclerosis Research
2009 - Springer Science+Business Media | Journal of Bioenergetics and Biomembranes
Charleen T. Chu, Edward D. Plowey, Ruben K. Dagda, Robert W. Hickey, Salvatore J. Cherra, Robert Clark,
Recent advances indicate that maintaining a balanced level of autophagy is critically important for neuronal health and function. Pathologic dysregulation of macroautophagy has been implicated in synaptic dysfunction, cellular stress, and neuronal cell death. Autophagosomes and autolysosomes are induced in acute and chronic neurological disorders including stroke, brain trauma, neurotoxin injury, Parkinson's, Alzheimer's, Huntington's, motor neuron, prion, lysosomal storage, and other neurodegenerative ...
Tópico(s): Parkinson's Disease Mechanisms and Treatments
2009 - Academic Press | Methods in enzymology on CD-ROM/Methods in enzymology
Chwan‐Li Shen, James K. Yeh, Jay Cao, Owatha L. Tatum, Raul Y. Dagda, Jia-Sheng Wang,
The purpose of this study was to explore the bioavailability, efficacy and molecular mechanisms of green tea polyphenols (GTP) related to preventing bone loss in rats with chronic inflammation. A 2 [placebo vs. lipopolysaccharide (LPS)]×2 (no GTP vs. 0.5% GTP in drinking water) factorial design enabled the evaluation of effects of LPS administration, GTP levels, and LPS×GTP interaction. Urinary GTP components and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels were determined by high-pressure liquid chromatography ...
Tópico(s): Bone health and osteoporosis research
2009 - Elsevier BV | The Journal of Nutritional Biochemistry
Ruben K. Dagda, Jianhui Zhu, Charleen T. Chu,
Alterations in mitochondrial biology have long been implicated in neurotoxin, and more recently, genetic models of parkinsonian neurodegeneration. In particular, kinase regulation of mitochondrial dynamics and turnover are emerging as central mechanisms at the convergence of neurotoxin, environmental and genetic approaches to studying Parkinson's disease (PD). Kinases that localize to mitochondria during neuronal injury include mitogen activated protein kinases (MAPK) such as extracellular signal ...
Tópico(s): Neurological diseases and metabolism
2009 - Elsevier BV | Mitochondrion
Ruben K. Dagda, Salvatore J. Cherra, Scott Kulich, Anurag Tandon, David S. Park, Charleen T. Chu,
Mitochondrial dysregulation is strongly implicated in Parkinson disease. Mutations in PTEN-induced kinase 1 (PINK1) are associated with familial parkinsonism and neuropsychiatric disorders. Although overexpressed PINK1 is neuroprotective, less is known about neuronal responses to loss of PINK1 function. We found that stable knockdown of PINK1 induced mitochondrial fragmentation and autophagy in SH-SY5Y cells, which was reversed by the reintroduction of an RNA interference (RNAi)-resistant plasmid ...
Tópico(s): Lysosomal Storage Disorders Research
2009 - Elsevier BV | Journal of Biological Chemistry
Salvatore J. Cherra, Ruben K. Dagda, Anurag Tandon, Charleen T. Chu,
Macroautophagy (hereafter, autophagy) plays a critical role in maintaining cellular homeostasis by degrading protein aggregates and dysfunctional/damaged organelles. We recently reported that silencing the recessive familial Parkinson disease gene encoding PTEN-induced kinase 1 (PINK1) leads to neuronal cell death accompanied by mitochondrial dysfunction and Drp1-dependent fragmentation. In this model, mitochondrial fission and Beclin 1-dependent autophagy play protective roles, cooperating to sequester ...
Tópico(s): Parkinson's Disease Mechanisms and Treatments
2009 - Taylor & Francis | Autophagy
Stefan Strack, Ralf Ruediger, Gernot Walter, Ruben K. Dagda, Chris Barwacz, J. Thomas Cribbs,
Protein serine/threonine phosphatase (PP) 2A is a ubiquitous enzyme with pleiotropic functions. Trimeric PP2A consists of a structural A subunit, a catalytic C subunit, and a variable regulatory subunit. Variable subunits (B, B′, and B" families) dictate PP2A substrate specificity and subcellular localization. B-family subunits contain seven WD repeats predicted to fold into a β-propeller structure. We carried out mutagenesis of Bγ to identify domains important for association with A and C subunits ...
Tópico(s): Ubiquitin and proteasome pathways
2002 - Elsevier BV | Journal of Biological Chemistry
Ruben K. Dagda, Jianhui Zhu, Scott Kulich, Charleen T. Chu,
Degenerating neurons of Parkinson’s disease (PD) patient brains exhibit granules of phosphorylated extracellular signal-regulated protein kinase 1/2 (ERK1/2) that localize to autophagocytosed mitochondria. Here we show that 6-hydroxydopamine (6-OHDA) elicits activity-related localization of ERK1/2 in mitochondria of SH-SY5Y cells, and these events coincide with induction of autophagy and precede mitochondrial degradation. Transient transfection of wild-type (WT) ERK2 or constitutively active MAPK/ERK ...
Tópico(s): Endoplasmic Reticulum Stress and Disease
2008 - Taylor & Francis | Autophagy
Eng‐King Tan, Anthony H.V. Schapira,
... Hum Mol Genet 2010; 19: 4861–4870. 15 Dagda RK, Cherra SJ III, Kulich SM, Tandon A, ...
Tópico(s): Neurological diseases and metabolism
2010 - Wiley | European Journal of Neurology
Chwan‐Li Shen, J. K. Yeh, Christina Samathanam, Jay Cao, Barbara J. Stoecker, Raul Y. Dagda, Ming‐Chien Chyu, Dale M. Dunn, J.-S. Wang,
Tópico(s): Bone health and treatments
2010 - Springer Science+Business Media | Osteoporosis International
Chwan‐Li Shen, J. K. Yeh, Jay Cao, Owatha L. Tatum, Raul Y. Dagda, J.-S. Wang,
Studies suggest that green tea polyphenols (GTP) or alphacalcidol is promising agent for preventing bone loss. Findings that GTP supplementation plus alphacalcidol administration increased bone mass via a decrease of oxidative stress and inflammation suggest a significant role of GTP plus alphacalcidol in bone health of patients with chronic inflammation. Studies have suggested that green tea polyphenols (GTP) or alphacalcidol are promising dietary supplements for preventing bone loss in women. However, ...
Tópico(s): Bone health and treatments
2010 - Springer Science+Business Media | Osteoporosis International
Frits Kamp, Nicole Exner, A. Kathrin Lutz, Nora Wender, Jan Hegermann, Bettina Brunner, Brigitte Nuscher, Tim Bartels, Armin Giese, Klaus Beyer, Stefan Eimer, Konstanze F. Winklhofer, Christian Haass,
... Valente et al, 2004; Exner et al, 2007; Dagda et al, 2009; Lutz et al, 2009; Morais ...
Tópico(s): Alzheimer's disease research and treatments
2010 - Springer Nature | The EMBO Journal
Ruben K. Dagda, Tamanna Sultana, James Lyons‐Weiler,
The availability of different scoring schemes and filter settings of protein database search algorithms has greatly expanded the number of search methods for identifying candidate peptides from MS/MS spectra. We have previously shown that consensus-based methods that combine three search algorithms yield higher sensitivity and specificity compared to the use of a single search engine (individual method). We hypothesized that union of four search engines (Sequest, Mascot, X!Tandem and Phenyx) can further ...
Tópico(s): Identification and Quantification in Food
2010 - OMICS Publishing Group | Journal of Proteomics & Bioinformatics
Chwan‐Li Shen, James K. Yeh, Christina Samathanam, Jay Cao, Barbara J. Stoecker, Raul Y. Dagda, Ming‐Chien Chyu, Jia-Sheng Wang,
Tópico(s): Curcumin's Biomedical Applications
2010 - Elsevier BV | The Journal of Nutritional Biochemistry