Humeyra Taskent‐Sezgin, Peter Marek, Rosanne Thomas, Daniel E. Goldberg, Juah Chung, Isaac S. Carrico, Daniel P. Raleigh,
p-Cyanophenylalanine is an extremely useful fluorescence probe of protein structure that can be recombinantly and chemically incorporated into proteins. The probe has been used to study protein folding, protein-membrane interactions, protein-peptide interactions, and amyloid formation; however, the factors that control its fluorescence are not fully understood. Hydrogen bonding to the cyano group is known to play a major role in modulating the fluorescence quantum yield, but the role of potential ...
Tópico(s): Advanced biosensing and bioanalysis techniques
2010 - American Chemical Society | Biochemistry
Humeyra Taskent‐Sezgin, Juah Chung, P. S. Banerjee, Sureshbabu Nagarajan, R. Brian Dyer, Isaac S. Carrico, Daniel P. Raleigh,
Highly sensitive: The azido analogue of methionine, azidohomoalanine (see picture), is shown to be a sensitive IR probe of protein structure, folding, and electrostatics, as demonstrated for ribosomal protein NTL9. It can be readily incorporated in to proteins, and the azido frequency is significantly blue-shifted in the thermally unfolded state. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. ...
Tópico(s): Photosynthetic Processes and Mechanisms
2010 - Wiley | Angewandte Chemie International Edition
Sureshbabu Nagarajan, Humeyra Taskent‐Sezgin, Dzmitry Parul, Isaac S. Carrico, Daniel P. Raleigh, R. Brian Dyer,
The time scale for ordering of the polypeptide backbone relative to the side chains is a critical issue in protein folding. The interplay between ordering of the backbone and ordering of the side chains is particularly important for the formation of β-sheet structures, as the polypeptide chain searches for the native stabilizing cross-strand interactions. We have studied these issues in the N-terminal domain of protein L9 (NTL9), a model protein with mixed α/β structure. We have developed a general ...
Tópico(s): Enzyme Structure and Function
2011 - American Chemical Society | Journal of the American Chemical Society
Renata Bordeira–Carriço, Daniel Ferreira, Denisa D. Mateus, Hugo Pinheiro, Ana Paula Pêgo, Manuel A. S. Santos, Carla Oliveíra,
Hereditary diffuse gastric cancer (HDGC) syndrome, although rare, is highly penetrant at an early age, and is severe and incurable because of ineffective screening tools and therapy. Approximately 45% of HDGC families carry germline CDH1/E-cadherin alterations, 20% of which are nonsense leading to premature protein truncation. Prophylactic gastrectomy is the only recommended approach for all asymptomatic CDH1 mutation carriers. Suppressor-tRNAs can replace premature stop codons (PTCs) with a cognate ...
Tópico(s): RNA and protein synthesis mechanisms
2014 - Springer Nature | European Journal of Human Genetics
Joana Rosado Coelho, João André Carriço, Daniel R. Knight, José Luis Martínez, Ian Morrissey, Marco R. Oggioni, Ana T. Freitas,
The rise of antibiotic resistance in pathogenic bacteria is a significant problem for the treatment of infectious diseases. Resistance is usually selected by the antibiotic itself; however, biocides might also co-select for resistance to antibiotics. Although resistance to biocides is poorly defined, different in vitro studies have shown that mutants presenting low susceptibility to biocides also have reduced susceptibility to antibiotics. However, studies with natural bacterial isolates are more ...
Tópico(s): Bacteriophages and microbial interactions
2013 - Public Library of Science | PLoS ONE
George R. Deeb, Ludmils Antonos, Samuel Tack, Caroline K. Carrico, Daniel M. Laskin, Janina Golob Deeb,
The use of cone-beam computed tomography (CBCT) for evaluation of patients for dental implants has gained considerable popularity. This retrospective cohort study was designed to determine whether using a clinical examination and a panoramic radiograph (Panorex) for implant selection and determining the need for bone grafting would be comparable to using CBCT in routine implant cases.Implant size and need for bone grafting were initially determined in 82 patients using a panoramic radiograph and clinical ...
Tópico(s): Endodontics and Root Canal Treatments
2016 - Elsevier BV | Journal of Oral and Maxillofacial Surgery
George R. Deeb, Bryan Wheeler, M. G. K. Jones, Caroline K. Carrico, Daniel M. Laskin, Janina Golob Deeb,
Purpose The more informed a patient is about a given procedure, the better the ultimate outcome. This study was designed to compare general public awareness and knowledge regarding oral implant treatment with those of patients presenting for such treatment and to determine the sources from which they may have obtained such information, as well as the accuracy of the information. Patients and Methods In this cross-sectional study, 2 groups of patients were asked to complete a questionnaire containing implant ...
Tópico(s): Dental Education, Practice, Research
2017 - Elsevier BV | Journal of Oral and Maxillofacial Surgery
Tiffany R. Glynn, Noelle A. Mendez, Deborah L. Jones, Sannisha K. Dale, Adam W. Carrico, Daniel J. Feaster, Allan Rodríguez, Steven A. Safren,
Individuals living with HIV report disproportionately high levels of trauma exposure and PTSD symptoms, both which have been associated with suboptimal ART adherence. Often conflated, the question arises as to which construct is driving subsequent HIV self-care behavior. Given the HIV disparities among Black and Hispanic/Latinx individuals, and that Miami is a geographic region with a high racial/ethnic minority make up and a unique socioeconomic environment, it is important to explore factors related ...
Tópico(s): Adolescent Sexual and Reproductive Health
2020 - Springer Science+Business Media | Journal of Behavioral Medicine
Janina Golob Deeb, Caroline K. Carrico, Daniel M. Laskin, Thomas E. Koertge,
Self‐assessments were introduced into a third‐year clinical periodontics course to enable dental students to self‐assess and receive faculty feedback prior to technical assessment. The aims of this study were to determine if there was an improvement in the pass rate and average grade on the technical assessments following implementation of self‐assessments and to analyze the accuracy of the student self‐assessments by comparing them with faculty assessments. Scores from 106 third‐year dental students ...
Tópico(s): Medical Education and Admissions
2019 - Wiley | Journal of Dental Education
Humeyra Taskent‐Sezgin, Juah Chung, P. S. Banerjee, Sureshbabu Nagarajan, R. Brian Dyer, Isaac S. Carrico, Daniel P. Raleigh,
Hoch empfindlich: Das Azido-Analogon von Methionin, Azidohomoalanin (siehe Bild), ist eine empfindliche IR-Sonde für die Analyse der Struktur, Faltung und Elektrostatik von Proteinen, wie am Beispiel des Proteins NTL9 demonstriert wird. Die Sonde lässt sich leicht in Proteine einbauen, und die Azido-Frequenz im thermisch entfalteten Zustand ist deutlichen blauverschoben.
Tópico(s): Protein Structure and Dynamics
2010 - Wiley | Angewandte Chemie
George R. Deeb, Graham H. Wilson, Caroline K. Carrico, Usman Zafar, Daniel M. Laskin, Janina Golob Deeb,
Tópico(s): Facial Trauma and Fracture Management
2016 - Elsevier BV | Journal of Oral and Maxillofacial Surgery
Janina Golob Deeb, Sompop Bencharit, Caroline K. Carrico, Marija Lukic, Daniel Hawkins, Ksenija Rener‐Sitar, George R. Deeb,
Abstract Introduction Recent computer‐guided dynamic navigation systems promise a novel training approach for implant surgery. This study aimed to examine learning progress in placement of dental implants among dental students using dynamic navigation on a simulation model. Materials and Methods Senior students with no implant placement experience were randomly assigned five implant placement attempts involving either three maxillary or four mandibular implants distributed in the anterior/posterior, ...
Tópico(s): Dental Radiography and Imaging
2019 - Wiley | European Journal Of Dental Education
Ivan Peran, Tracey A. Oudenhoven, Ann Marie Woys, Matthew D. Watson, Tianqi O. Zhang, Isaac S. Carrico, Martin T. Zanni, Daniel P. Raleigh,
A high-sensitivity metal-carbonyl-based IR probe is described that can be incorporated into proteins or other biomolecules in very high yield via Click chemistry. A two-step strategy is demonstrated. First, a methionine auxotroph is used to incorporate the unnatural amino acid azidohomoalanine at high levels. Second, a tricarbonyl (η(5)-cyclopentadienyl) rhenium(I) probe modified with an alkynyl linkage is coupled via the Click reaction. We demonstrate these steps using the C-terminal domain of the ...
Tópico(s): Photosynthetic Processes and Mechanisms
2014 - American Chemical Society | The Journal of Physical Chemistry B
George R. Deeb, Sarah Braun, Caroline K. Carrico, Patricia A. Kinser, Daniel M. Laskin, Janina Golob Deeb,
The aim of this study was to assess the relationship between burnout, depressive symptoms and suicidal ideation in dental and dental hygiene students and to evaluate the influence of gender, programme type and year of study.Third- and fourth-year dental (DS) and first- and second-year hygiene students (DHS) completed the Patient Health Questionnaire (PHQ-9) and an abbreviated Maslach Burnout Inventory online as measures of depressive symptoms/suicidality and burnout, respectively. The statistical analyses ...
Tópico(s): Stress and Burnout Research
2017 - Wiley | European Journal Of Dental Education
George R. Deeb, Dan Tran, Caroline K. Carrico, E. J. Block, Daniel M. Laskin, Janina Golob Deeb,
Purpose The tent screw pole technique is one of the methods available for practitioners to perform horizontal ridge augmentation to facilitate dental implant placement. The purpose of this study was to evaluate the efficacy of the tent screw pole technique for horizontal ridge augmentation and to compare the results with those of the tunnel technique and open ridge augmentation. Patients and Methods In this retrospective cohort study, 35 patients underwent horizontal ridge augmentation with the ...
Tópico(s): Dental Radiography and Imaging
2017 - Elsevier BV | Journal of Oral and Maxillofacial Surgery
Dean M. DeLuke, Vickas Agarwal, Trevor Holleman, Caroline K. Carrico, Daniel M. Laskin,
Purpose During the past 2 decades, there has been a marked decrease in the willingness of community-based oral and maxillofacial surgeons to participate in trauma call. Although many factors can influence the decision not to take trauma call, 1 primary disincentive is the perception that managing facial trauma might be profitable for the hospital, but not profitable for the surgeon. The purpose of this study was to compare the profitability of facial trauma management for the hospital and the surgeon ...
Tópico(s): Medical Malpractice and Liability Issues
2016 - Elsevier BV | Journal of Oral and Maxillofacial Surgery
Timothy L. Wiemken, Stephen Furmanek, Ruth Carrico, Paula Peyrani, Daniel F. Hoft, Alicia M. Fry, Julio A. Ramírez,
Abstract Background Influenza is associated with excess morbidity and mortality of individuals each year. Few therapies exist for treatment of influenza infection, and each require initiation as early as possible in the course of infection, making efficacy difficult to estimate in the hospitalized patient with lower respiratory tract infection. Using causal machine learning methods, we re-analyze data from a randomized trial of oseltamivir versus standard of care aimed at reducing clinical failure ...
Tópico(s): Hepatitis C virus research
2021 - BioMed Central | BMC Infectious Diseases
Dean M. DeLuke, Darriel Cannon, Caroline K. Carrico, Ben Byrne, Daniel M. Laskin,
Administration of safe and effective local anesthesia is a cornerstone of dental practice, but there is some discrepancy in recommendations for maximum doses, with the Council on Dental Therapeutics and American Academy of Pediatric Dentistry's guidelines differing from the guidelines of manufacturers and contemporary textbooks. The aim of this study was to determine the level of uniformity across U.S. dental schools in teaching maximal safe doses for commonly used local anesthetics. Faculty members ...
Tópico(s): Anesthesia and Pain Management
2018 - Wiley | Journal of Dental Education
Alyssa Franklin, Caroline K. Carrico, Daniel M. Laskin,
Previous studies have shown that there may be a bias among some male oral and maxillofacial surgeons regarding women practicing in the specialty. The purpose of this study was to determine if there is a similar bias in the general public.We performed a survey of 65 patients of various genders, educational levels, and ages asking them their surgeon gender preference for extraction of a tooth, removal of impacted third molars, and cosmetic surgery, as well as the reasons for their preference.Of respondents, ...
Tópico(s): Medical Education and Admissions
2016 - Elsevier BV | Journal of Oral and Maxillofacial Surgery
Janina Golob Deeb, Amy Reichert, Caroline K. Carrico, Daniel M. Laskin, George R. Deeb,
The purpose of this study was to investigate if the addition of biologic agents to a particulate bone graft enhances horizontal ridge augmentation outcomes in terms of bone dimensions, bone density, and successful implant placement.A retrospective chart review was done to assess the clinical and radiographic outcomes in 52 horizontal ridge augmentation sites in 43 patients. Information was gathered regarding surgical technique, type of graft material, biologic agents used (PRP or rhPDGF-BB), method ...
Tópico(s): dental development and anomalies
2020 - Wiley | Clinical and Experimental Dental Research
Bryan Wheeler, Caroline K. Carrico, Bhavna Shroff, Tegwyn H. Brickhouse, Daniel M. Laskin,
There is considerable controversy in the literature concerning the indications for frenectomy for treating a maxillary diastema and for timing of the procedure. The purpose of this study was to survey pediatric dentists, orthodontists, and oral and maxillofacial surgeons on their opinion of this matter to develop a consensus.An anonymous 7-item electronic questionnaire was sent to members of the American Academy of Pediatric Dentists, the American Association of Orthodontists, and the American Association ...
Tópico(s): Cleft Lip and Palate Research
2017 - Elsevier BV | Journal of Oral and Maxillofacial Surgery
Ivan Peran, Alex S. Holehouse, Isaac S. Carrico, Rohit V. Pappu, Osman Bilsel, Daniel P. Raleigh,
Significance The tools of structural biology afford high-resolution descriptions of folded states of proteins. However, an atomic-level description of unfolded states under folding conditions has remained elusive. Challenges arise from the pronounced conformational heterogeneity and the very low population of unfolded states under folding conditions. We have combined a series of time-resolved biophysical experiments with all-atom simulations and polymer theory to obtain a high-resolution description ...
Tópico(s): RNA and protein synthesis mechanisms
2019 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Brennen T. Olsen, Connor J. Sherwood, Caroline K. Carrico, James H. Priest, Daniel M. Laskin,
Purpose Experience has shown that in malpractice cases patients often claim certain information was not provided during the informed consent process. The purposes of this study were to determine how much of an informed consent video patients initially remember and how much is recalled when there is a hiatus from viewing the video to the surgery. Patients and Methods In this prospective cohort study, patients presenting for impacted third molar removal in an academic setting (n = 50) or a private office ( ...
Tópico(s): Social Media in Health Education
2017 - Elsevier BV | Journal of Oral and Maxillofacial Surgery
Produção Nacional
Maria Luíza M. Silva, Ilana Zalcberg, Maria Helena Ornellas de Souza, Felippe V. Simões, Kadma Carriço, Daniel Tabak, Paul C. Ribeiro, Eliana Abdelhay,
Tópico(s): T-cell and Retrovirus Studies
1996 - Elsevier BV | Cancer Genetics and Cytogenetics
Produção Nacional
Revisado por pares
Camila Santana Carriço, Fernanda T. Cruz, Maurício B. dos Santos, Daniel Souza de Oliveira, Heloise O. Pastore, Heloysa Martins Carvalho Andrade, Artur J.S. Mascarenhas,
The materials derived from MWW layered precursor (MCM-22, MCM-36 and ITQ-2) were synthesized with molar ratio SiO2/Al2O3 = 30, characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetry (TG), scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDX), textural analysis by N2 physisorption, temperature programmed desorption of ammonia (NH3-TPD) and diffuse reflectance infrared spectroscopy (DRIFTS) of adsorbed pyridine and evaluated in gas ...
Tópico(s): Mesoporous Materials and Catalysis
2015 - Elsevier BV | Journal of Catalysis
Humeyra Taskent‐Sezgin, Juah Chung, Vadim Patsalo, Shigeki J. Miyake-Stoner, Andrew M. Miller, Scott H. Brewer, Ryan A. Mehl, David F. Green, Daniel P. Raleigh, Isaac S. Carrico,
The use of noncoded amino acids as spectroscopic probes of protein folding and function is growing rapidly, in large part because of advances in the methodology for their incorporation. Recently p-cyanophenylalanine has been employed as a fluorescence and IR probe, as well as a FRET probe to study protein folding, protein−membrane interactions, protein−protein interactions and amyloid formation. The probe has been shown to be exquisitely sensitive to hydrogen bonding interactions involving the cyano ...
Tópico(s): Enzyme Structure and Function
2009 - American Chemical Society | Biochemistry
Daniel G. Maluf, Robert J. Carrico, J.D. Rosendale, Richard V. Perez, Sandy Feng,
The optimal balance between maximizing the number versus the outcome of transplantation utilizing kidneys from small (≤20 kg) pediatric donors remains unclear, complicated by the choice of single versus en bloc transplantation with their attendant technical risks. Using the Organ Procurement and Transplantation Network (OPTN) database, we examined kidney recovery and utilization patterns, and 1-year transplant outcomes by single kilogram weight strata. Between January 1, 2005 and June 30, 2010, 2352 ...
Tópico(s): Organ Donation and Transplantation
2013 - Elsevier BV | American Journal of Transplantation
Daniel M. Laskin, Caroline K. Carrico, Janelle M. Wood,
Abstract A common predictive measure of postoperative nausea and vomiting (PONV) is the Apfel score. Although tested in many different operations, it has not been tested extensively in oral and maxillofacial surgery (OMFS). This study was designed to determine whether it applied to OMFS and whether there were other factors in this population that would improve its accuracy. A retrospective chart review was carried out on a randomly selected group of patients who had OMFS during a 10-month period. In addition ...
Tópico(s): Anesthesia and Sedative Agents
2019 - Elsevier BV | International Journal of Oral and Maxillofacial Surgery
George R. Deeb, Amber E. Johnson, Mikhail Bondarew, Caroline K. Carrico, Daniel M. Laskin, Janina Golob Deeb,
The purpose of this study was to evaluate the effect of a three-hour hands-on workshop for medical students and residents on their pre- and postcourse knowledge and confidence in managing dental emergencies. A 1-hour lecture followed by four 20-minute "hands-on" skill stations on dental mannequins was administered to a group of 30 medical students and residents. Pre- and postworkshop questionnaire surveys were conducted. There was a significant increase in the percent of attendees who responded correctly ...
Tópico(s): Cardiac Arrest and Resuscitation
2016 - SAGE Publishing | Journal of Medical Education and Curricular Development
Soheil Rostami, Balraj Kang, Eser Tüfekçi, Spiro Stilianoudakis, Caroline K. Carrico, Daniel M. Laskin,
Purpose Studies have shown that an asymmetrical smile is a relatively common problem. Yet, many patients are unaware of having this condition. Because having an asymmetrical smile can affect the final esthetic result of orthodontic therapy or orthognathic surgery, such patients need to be aware of the problem. The purpose of this study was to determine what amount of smile asymmetry is clinically evident to orthodontists, oral and maxillofacial surgeons (OMSs), and the lay public. Materials and Methods ...
Tópico(s): Body Image and Dysmorphia Studies
2019 - Elsevier BV | Journal of Oral and Maxillofacial Surgery