Florence Noël, Daniel Prati, Mark van Kleunen, Andreas Gygax, Daniel Moser, Markus Fischer,
Tópico(s): Species Distribution and Climate Change
2010 - Elsevier BV | Biological Conservation
Pascal Gygax, Daniel Elmiger, Sandrine Zufferey, Alan Garnham, Sabine Sczesny, Lisa von Stockhausen, Friederike Braun, Jane Oakhill,
Psycholinguistic investigations of the way readers and speakers perceive gender have shown several biases associated with how gender is linguistically realized in language. Although such variations across languages offer interesting grounds for legitimate cross-linguistic comparisons, pertinent characteristics of grammatical systems - especially in terms of their gender asymmetries - have to be clearly identified. In this paper, we present a language index for researchers interested in the effect of ...
Tópico(s): Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
2019 - Frontiers Media | Frontiers in Psychology
Herbert Waldmann, Daniel Gygax, Mark D. Bednarski, W. Randall Shangraw, George M. Whitesides,
Tópico(s): Microbial Metabolites in Food Biotechnology
1986 - Elsevier BV | Carbohydrate Research
Daniel Gygax, Peter Spies, Tammo Winkler, Ulrike Pfaar,
β-D-glucuronides of 1 and 2 have been synthesized by a multi-enzyme system with in situ regeneration of uridine 5′-diphosphoglucuronic acid. Crude liver homogenate containing all enzymes involved in the multi-enzyme system was used for this stereoselective one-pot reaction.
Tópico(s): Glycogen Storage Diseases and Myoclonus
1991 - Elsevier BV | Tetrahedron
Udo Kragl, Daniel Gygax, Oreste Ghisalba, Christian Wandrey,
Die Verwendung von N ‐Acetylglucosamin‐2‐Epimerase aus Schweinenieren zusammen mit N ‐Acetylneuraminsäure‐Aldolase führt zu einem besonders einfachen und effektiven Verfahren zur Herstellung von N ‐Acetylneuraminsäure 4 aus N ‐Acetylglucosamin 1 und Brenztraubensäure 3 . Beide Enzyme, homogen gelöst im Enzym‐Membranreaktor, katalysieren im kontinuierlichen Prozeß die Synthese von N ‐Acetylneuraminsäure 4 (über 2 ) mit einer Raum‐Zeit‐Ausbeute von 109 g L −1 d −1 . magnified image
Tópico(s): Enzyme Structure and Function
1991 - Wiley | Angewandte Chemie
Udo Kragl, Daniel Gygax, Oreste Ghisalba, Christian Wandrey,
Abstract For corrigendum see DOI: 10.1002/anie.199117131
Tópico(s): Enzyme Production and Characterization
1991 - Wiley | Angewandte Chemie International Edition
Elke Schmidt, Oreste Ghisalba, Daniel Gygax, Gottfried Sedelmeier,
(R)-2-Hydroxy-4-phenylbutyric acid, an intermediate in the manufacture of inhibitors of angiotensin converting enzyme, can be produced continuously in an enzyme membrane reactor by enzymatic reduction of its corresponding alpha-keto acid. D-Lactate dehydrogenase (D-LDH) from Staphylococcus epidermidis was chosen as the most appropriate enzyme to carry out the NADH-dependent reduction. Formate dehydrogenase (FDH) was used for NADH regeneration. Detailed kinetic measurements and a mathematical model ...
Tópico(s): Metabolism and Genetic Disorders
1992 - Elsevier BV | Journal of Biotechnology
C. Pfister, M. Duval, J. Godbillon, G. Gosset, Daniel Gygax, F. Marfil, A. Sioufi, Bruce C. Winkler,
CGS 20 267 is a new potent and selective, nonsteroidal, oral aromatase inhibitor. For its determination in human plasma and urine, an enzyme immunoassay (EIA) and an HPLC method were developed. The EIA showed good precision and accuracy (intra- and interassay variation between 3.0 and 17.7%, recoveries between 81 and 106%) and a quantitation limit of 0.7 nmol/L. A strong cross reactivity of the antibodies with the hydroxy metabolite of CGS 20 267 (CGP 44 645) was observed. The HPLC method showed a ...
Tópico(s): Asthma and respiratory diseases
1994 - Elsevier BV | Journal of Pharmaceutical Sciences
Elizaveta Fasler‐Kan, Claudia Suenderhauf, Natasha S. Barteneva, Birk Poller, Daniel Gygax, Jörg Huwyler,
Brain microvascular endothelial cells are part of the blood-brain barrier and participate actively in immunological processes including cytokine-mediated inflammatory reactions. Using the human brain capillary endothelial cell line hCMEC/D3, activation of JAK/STAT signaling pathways were studied in response to stimulation by cytokines. The phenotype of hCMEC/D3 cells was confirmed by flow cytometry analysis of cell adhesion factors (cluster of differentiation molecules CD31 and CD34) and the von ...
Tópico(s): RNA regulation and disease
2010 - Elsevier BV | Brain Research
Lara Hasan, Thomas M. Jermann, Jakob M. Weber, L. Abrahamsson, Michel‐Angelo Sciotti, Michael Böttcher, W. Jöchle, Daniel Gygax, André Scholer,
Gamma-hydroxybutyric acid (GHB) has become one of the most dangerous illicit drugs of abuse today. It is used as a recreational and date rape drug because of its depressant effect on the central nervous system, which may cause euphoria, amnesia, respiratory arrest, and coma. There is an urgent need for a simple, easy-to-use assay for GHB determination in urine and blood. In this article, a rapid enzymatic assay adapted to clinical chemistry analyzers for the detection of GHB is presented.The described ...
Tópico(s): Tea Polyphenols and Effects
2011 - Lippincott Williams & Wilkins | Therapeutic Drug Monitoring
Vanessa Rullaud, Michael Siragusa, Alessandro Cumbo, Daniel Gygax, Patrick Shahgaldian,
An amphiphilic calix[4]arene derivative bearing four guanidino moieties at the upper rim and four dodecyl chains at the lower rim was shown to form stable solid lipid nanoparticles (SLNs) in water. The study of the interactions of these cationic SLNs with DNA revealed a sequence-dependent groove binding mechanism.
Tópico(s): Advanced biosensing and bioanalysis techniques
2012 - Royal Society of Chemistry | Chemical Communications
Regine Landmann, R. Obrist, H. Denz, Christian Ludwig, Heiner Frost, M. Wesp, Christine Rordorf, Harry Towbin, Daniel Gygax, L. Tarcsay, J.P. Obrecht,
Tópico(s): Immune Response and Inflammation
1993 - Springer Science+Business Media | Biotherapy
Brigitte Gay, Jean-Michel A. Cardot, Christian Schnell, Peter van Hoogevest, Daniel Gygax,
□ The comparative pharmacokinetics of free MTP-PE (muramyl tripeptide phosphatidyl ethanolamine) and MTP-PE entrapped in negatively charged multilamellar liposomes (liposomal MTP-PE) was evaluated in rats at a bolus intravenous (iv) dose of 0.2 mg/kg and in dogs at a bolus iv dose of 0.1 mg/kg. Additional studies were performed with the free form in rats (1.4 mg/kg, bolus iv) and dogs (1 mg/kg, bolus iv) and with the liposomal form in dogs (0.5 mg/kg, bolus iv). Plasma samples were obtained at various times ...
Tópico(s): Advanced biosensing and bioanalysis techniques
1993 - Elsevier BV | Journal of Pharmaceutical Sciences
Anissa Kempf, Bjoern Tews, Michael E. Arzt, Oliver Weinmann, Franz J. Obermair, Vincent Pernet, Marta Zagrebelsky, Andrea Delekate, Cristina Iobbi, Ajmal Zemmar, Zorica Ristic, Miriam Gullo, Peter Spies, Dana A. Dodd, Daniel Gygax, Martin Körte, Martin E. Schwab,
Nogo-A is a membrane protein of the central nervous system (CNS) restricting neurite growth and synaptic plasticity via two extracellular domains: Nogo-66 and Nogo-A-Δ20. Receptors transducing Nogo-A-Δ20 signaling remained elusive so far. Here we identify the G protein-coupled receptor (GPCR) sphingosine 1-phosphate receptor 2 (S1PR2) as a Nogo-A-Δ20-specific receptor. Nogo-A-Δ20 binds S1PR2 on sites distinct from the pocket of the sphingolipid sphingosine 1-phosphate (S1P) and signals via the G protein ...
Tópico(s): Neuroscience and Neuropharmacology Research
2014 - Public Library of Science | PLoS Biology
Florian Cymer, Marco Thomann, Harald Wegele, Cécile Avenal, Tilman Schlothauer, Daniel Gygax, Hermann Beck,
Oxidation of monoclonal therapeutic antibodies (mAbs) can affect binding to Fc-receptors and potentially influence pharmacokinetics or effector functions like e.g. antibody dependent cellular phagocytosis (ADCP). Recently, it has been demonstrated that binding to FcγRIIa (H131) is affected by methionine oxidation of the Fc-portion but it is currently unknown which methionine is responsible for decreased binding. We separated an oxidized IgG1 monoclonal antibody based on the oxidation state of methionine ...
Tópico(s): T-cell and B-cell Immunology
2017 - Elsevier BV | Biologicals
Iwan Zimmermann, Pascal Egloff, Cedric A. J. Hutter, Fabian Arnold, Peter Stohler, Nicolas Bocquet, Melanie N. Hug, Sylwia Huber, M. Sloan Siegrist, Lisa Hetemann, Jennifer Gera, Samira Gmür, Peter Spies, Daniel Gygax, Eric R. Geertsma, Roger Dawson, Markus A. Seeger,
Mechanistic and structural studies of membrane proteins require their stabilization in specific conformations. Single domain antibodies are potent reagents for this purpose, but their generation relies on immunizations, which impedes selections in the presence of ligands typically needed to populate defined conformational states. To overcome this key limitation, we developed an in vitro selection platform based on synthetic single domain antibodies named sybodies. To target the limited hydrophilic ...
Tópico(s): RNA and protein synthesis mechanisms
2018 - eLife Sciences Publications Ltd | eLife
Amy Racine-Poon, L Botta, Tse Wen Chang, Frances M. Davis, Daniel Gygax, R S Liou, Patricia Rohane, Theophil Staehelin, Augusta M. P. van Steijn, William O. Frank,
The efficacy, pharmacodynamics, and pharmacokinetics of CGP 51901, a recombinant monoclonal mouse—human chimeric anti-human immunoglobulin E (IgE) antibody were evaluated for 153 patients with seasonal allergic rhinitis treated with placebo or with 15, 30, or 60 mg CGP 51901 in six biweekly doses. Seasonal allergic rhinitis was chosen to validate the concept of anti-IgE therapy because the causal and temporal relation between allergen confrontation and IgE-mediated evocation of symptoms is firmly ...
Tópico(s): Inhalation and Respiratory Drug Delivery
1997 - Wiley | Clinical Pharmacology & Therapeutics
Jonathan Corne, Ratko Djukanović, Larry L. Thomas, Jill A. Warner, L Botta, BM Grandordy, Daniel Gygax, Christoph Heusser, Francesco Patalano, William H. Richardson, E. Kilchherr, T. Staehelin, Frances M. Davis, Wayne L. Gordon, Lee K. Sun, R S Liou, G Wang, Tse Wen Chang, S T Holgate,
CGP 51901 is a non-anaphylactogenic mouse/human chimeric anti-human IgE antibody that binds to free IgE and surface IgE of IgE-expressing B cells but not to IgE bound to high affinity IgE receptors (Fc epsilonR1) on mast cells and basophils or low affinity IgE receptors (Fc epsilonR2) on other cells. A phase 1 double-blind, placebo-controlled, single dose study with doses of 3, 10, 30, and 100 mg of CGP 51901 was conducted in 33 pollen-sensitive subjects who had raised levels of serum IgE and received ...
Tópico(s): Allergic Rhinitis and Sensitization
1997 - American Society for Clinical Investigation | Journal of Clinical Investigation
Robert H. Schneider, Mario Hammel, Eric G. Berger, Oreste Ghisalba, Jakob Nueesch, Daniel Gygax,
Tópico(s): Animal Genetics and Reproduction
1990 - Springer Science+Business Media | Glycoconjugate Journal
Daniel Gygax, Oreste Ghisalba, H.J. Treichler, J. Nüesch,
Tópico(s): Mycobacterium research and diagnosis
1990 - Springer Nature | The Journal of Antibiotics
Daniel Gygax, L Botta, Markus Ehrat, Peter Gräf, Gilbert Lefèvre, Peter Oroszlán, C. Pfister,
Summary For the evaluation and interpretation of pharmacokinetic data reliable quantitative determinations are a requirement that can only be met by well-characterized and fully validated analytical methods. To cope with these requirements a method is being established that is based on an integrated and automated fiber-optic biospecific interaction analysis system (FOBIA) for immunoassays. Performance characteristics of this system used in monitoring of recombinant hirudin (CGP 39 393) are presented. ...
Tópico(s): Biosimilars and Bioanalytical Methods
1996 - Lippincott Williams & Wilkins | Therapeutic Drug Monitoring
Thea Ryhner, Max M. Wittenbrink, Dagmar Nitzl, Simon L. Zeller, Daniel Gygax, Mustafa Eser,
In this case report a 10 year old Freiberger mare with a Mycobacterium avium subsp. avium infection is presented. This infection leads to a tuberculosis like disease with granulomatous alterations particularly of the intestines and lungs and is only sporadically reported in horses of Central Europe. Diarrhoea, mastitis and neck stiffness as well as dyspnoea and chronic cough are more specific symptoms of the infection, while weight loss, weakness and lethargy are nonspecific signs. As these clinical ...
Tópico(s): Infectious Diseases and Mycology
2009 - Society of Swiss Veterinarians | Schweizer Archiv für Tierheilkunde
A. Schenck, F. N. Gygax, Daniel Andreica, Y. O ̄nuki,
We report on measurements of the Knight shift K and spin–lattice relaxation rate λ of positive muons (μ+) implanted in cubic CeAg, a compound which is known to possess ferromagnetic ( K) and ferroquadrupolar (TQ = 15–16 K) order. The zero field (ZF) λ implies randomly and isotropically fluctuating Ce 4f moments above TQ which show a trend to a, perhaps critical, slowing down on approaching TQ. Below TQ the fluctuations are restricted to the now tetragonally distorted axis of CeAg. In a longitudinal field ...
Tópico(s): Inorganic Chemistry and Materials
2003 - IOP Publishing | Journal of Physics Condensed Matter
Daniel Gygax, Marbeth Christ, Oreste Ghisalba, Jakob NÃ ⁄ esch,
Tópico(s): Microbial Metabolites in Food Biotechnology
1982 - Oxford University Press | FEMS Microbiology Letters
Claudia Escher, Hanns Lochmüller, Dirk Fischer, Stephan Frank, Jens Reimann, Maggie C. Walter, Markus Ehrat, Markus A. Rüegg, Daniel Gygax,
The definite molecular diagnosis in patients with muscular dystrophies often requires the assessment of muscular expression of multiple proteins in small amounts of muscle tissue. The sample material obtained in muscle biopsies is limited and the measurement of multiple proteins is often restricted to conventional, non-quantitative assays, i.e. immunohistochemistry and immunoblotting. Here, we demonstrate that reverse protein arrays are a novel and excellent material-saving method for the measurement ...
Tópico(s): Virus-based gene therapy research
2010 - Elsevier BV | Neuromuscular Disorders
René Lattmann, Oreste Ghisalba, Daniel Gygax, Hans‐Peter Schär, Elke Schmidt,
A number of enzymes with reasonable to excellent selectivities for the conversion of prochiral 2-benzylglyceroldiacetate 1c into optically active monoacetate 2c could be identified in a screening of microbial esterases and lipases. For the synthesis of the S-enantiomer of 2c by hydrolysis, two enzymes (from Aspergillus fumigatus and from Mucor javanicus) were identified which give the product with an e.e.-value of 75%. The R-enantiomer can be obtained with a purified lipase/esterase from Pseudomonas ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
1990 - Taylor & Francis | Biocatalysis
Joël Zindel, Chantal Gygax, Peter Studer, Melanie Kauper, Daniel Candinas, Vanessa Banz, Lukas Brügger,
Defunctioning loop ileostomies (LI) are commonly used in colorectal surgery to reduce the potentially detrimental consequences of anastomotic leakages. However, stoma-related morbidity is high with up to 75% of patients having local complications. The aim of this study was to investigate the effect of a sustaining rod on the local complication rate.In this prospective, multi-center, randomized controlled trial, subjects were allocated to either a rod or a rod-less protocol (NCT00959738). The primary ...
Tópico(s): Anorectal Disease Treatments and Outcomes
2017 - Springer Science+Business Media | International Journal of Colorectal Disease
Elizaveta Fasler‐Kan, Natasha S. Barteneva, Sylvia Ketterer, Kerstin Wunderlich, Jörg Huwyler, Daniel Gygax, Josef Flammer, Peter Meyer,
Background: Retinal pigment epithelial cells constitute an important component of the blood–retinal barrier and play a pivotal role in the development of age-related macular degeneration (AMD). Understanding the underlying molecular mechanisms is a prerequisite for developing therapeutic strategies for the treatment of this disease. This study investigated cytokine-induced changes of the JAK–STAT (Janus tyrosine kinase–signal transducers and activators of transcription) signaling pathway in the human ...
Tópico(s): Systemic Lupus Erythematosus Research
2010 - Dove Medical Press | International Journal of Interferon Cytokine and Mediator Research
Daniel Vonwil, David Wendt, Simon Ströbel, Hans‐Joachim Wallny, Daniel Gygax, M. Heberer, Iván Martín,
In order to develop suitable bioreactor systems and processes for automated and standardized cell cultures involving the use of bioactive factors, we determined the stability of transforming growth factor beta 3 (TGFβ3) over storage time and under conditions typically used for mammalian cell culture. Using a reporter gene assay with firefly luciferase as readout, significant reduction of TGFβ3 bioactivity was detected to occur both in serum containing medium (SCM) and serum free medium (SFM). The residual ...
Tópico(s): Osteoarthritis Treatment and Mechanisms
2007 - Elsevier BV | Biochemical Engineering Journal
F.N. Gygax, Daniel Andreica, A. Schenck, Yoshichika Ōnuki,
The magnetic structure of GdCu2 has been studied by zero-field μSR spectroscopy on a single crystal between 5 K and TN=39.6K. We find four distinct local fields composed of two widely spaced and narrowly split doublets at the μ+ position, determined to be a 4e interstitial site. The temperature dependencies of these local fields are peculiar. The four components decrease following precisely various unexpected −T3/2 relations up to almost 23TN, then they drop faster to reach zero at TN. At least up ...
Tópico(s): Rare-earth and actinide compounds
2002 - Elsevier BV | Journal of Magnetism and Magnetic Materials