Krista M. D. La Perle, F Silveria, David E. Anderson, Eric A.G. Blomme,
Disseminated eosinophilic myositis was diagnosed in an alpaca that had been imported to the USA from Peru 5 years earlier. The myositis was associated with macroscopically visible large sarcocysts that were characterized histologically by septate compartments containing bradyzoites, and ultraÍstructurally by cyst walls composed of anastomosing villous protrusions. Two hours before death, the alpaca aborted an 8-month-gestation fetus, but no lesions were found in the uterus, placenta or fetus. Additional ...
Tópico(s): Eosinophilic Disorders and Syndromes
1999 - Elsevier BV | Journal of Comparative Pathology
Jonathan T. Sockolosky, Eleonora Trotta, Giulia Parisi, Lora K. Picton, Leon Su, Alan Le, Akanksha Chhabra, Stephanie Silveria, Benson M. George, Chris King, Matthew Tiffany, Kevin M. Jude, Leah V. Sibener, David Baker, Judith A. Shizuru, Antoni Ribas, Jeffrey A. Bluestone, K. Christopher García,
Interleukin-2 (IL-2) is a cytokine required for effector T cell expansion, survival, and function, especially for engineered T cells in adoptive cell immunotherapy, but its pleiotropy leads to simultaneous stimulation and suppression of immune responses as well as systemic toxicity, limiting its therapeutic use. We engineered IL-2 cytokine-receptor orthogonal (ortho) pairs that interact with one another, transmitting native IL-2 signals, but do not interact with their natural cytokine and receptor ...
Tópico(s): Immunotherapy and Immune Responses
2018 - American Association for the Advancement of Science | Science
Xi He, Shuo Quan, Min Xu, Silveria Rodriguez, Shih Lin Goh, Jiajie Wei, Arthur Fridman, Kenneth A. Koeplinger, Steve S. Carroll, Jay A. Grobler, Amy S. Espeseth, David B. Olsen, Daria J. Hazuda, Dai Wang,
Significance The urgency of curbing the COVID-19 pandemic has motivated many investigators to pivot their research to understand the basic biology of SARS-CoV-2 and to search for new pharmaceutical compounds for potential COVID-19 treatment. However, most SARS-CoV-2 studies require biosafety level 3 facilities, which are in high demand, costly, and difficult to access. To overcome these limitations, we engineered a SARS-CoV-2 replicon, which is a modified virus subgenome capable of self-replicating ...
Tópico(s): Respiratory viral infections research
2021 - National Academy of Sciences | Proceedings of the National Academy of Sciences
David Wang, Jason Quiros, Kelly Mahuron, Chien-Chun Steven Pai, Valeria Ranzani, Arabella Young, Stephanie Silveria, Tory Harwin, Arbi Abnousian, Massimiliano Pagani, Michael D. Rosenblum, Frédéric Van Gool, Lawrence Fong, Jeffrey A. Bluestone, Michel DuPage,
Regulatory T cells (Tregs) are critical for maintaining immune homeostasis, but their presence in tumor tissues impairs anti-tumor immunity and portends poor prognoses in cancer patients. Here, we reveal a mechanism to selectively target and reprogram the function of tumor-infiltrating Tregs (TI-Tregs) by exploiting their dependency on the histone H3K27 methyltransferase enhancer of zeste homolog 2 (EZH2) in tumors. Disruption of EZH2 activity in Tregs, either pharmacologically or genetically, drove ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2018 - Cell Press | Cell Reports
Pieter Mestdagh, Nicole Hartmann, Lukas Baeriswyl, Ditte Andreasen, Nathalie Bernard, Caifu Chen, David L. Cheo, Petula D'Andrade, Mike DeMayo, Lucas Dennis, Stefaan Derveaux, Yun Feng, Stephanie Fulmer-Smentek, Bernhard Gerstmayer, Julia S. Gouffon, Chris Grimley, Eric Lader, Kathy Lee, Shujun Luo, Peter Mouritzen, Aishwarya Narayanan, Sunali Patel, Sabine Peiffer, Silvia Rüberg, Gary P. Schroth, Dave Schuster, Jonathan M. Shaffer, Elliot J Shelton, Scott Silveria, Umberto Ulmanella, Vamsi Veeramachaneni, Frank Staedtler, Thomas Peters, Toumy Guettouche, Linda Wong, Jo Vandesompele,
Tópico(s): Cancer-related molecular mechanisms research
2014 - Nature Portfolio | Nature Methods
Michel DuPage, David Wang, Jason Quiros, Kelly Mahuron, Chien-Chun Steven Pai, Valeria Ranzani, Arabella Young, Stephanie Silveria, Tory Harwin, Arbi Abnousian, Massimiliano Pagani, Michael Rosenblum, Frédéric Van Gool, Lawrence Fong, Jeffrey A. Bluestone,
Abstract Immunosuppressive regulatory T cells (Tregs) are critical for maintaining immune homeostasis, but their presence in tumor tissues impairs anti-tumor immunity and portends poor prognoses in cancer patients. Targeting Tregs may be a powerful means to unleash more potent immune responses against cancer, but targeting these cells is challenging because their generalized inactivation may incite severe autoimmune toxicities. To selectively target Tregs in tumors, we investigated the role of the ...
Tópico(s): T-cell and B-cell Immunology
2018 - American Association of Immunologists | The Journal of Immunology
Pieter Mestdagh, Nicole Hartmann, Lukas Baeriswyl, Ditte Andreasen, Nathalie Bernard, Caifu Chen, David L. Cheo, Petula D'Andrade, Mike DeMayo, Lucas Dennis, Stefaan Derveaux, Yun Feng, Stephanie Fulmer-Smentek, Bernhard Gerstmayer, Julia S. Gouffon, Chris Grimley, Eric Lader, Kathy Lee, Shujun Luo, Peter Mouritzen, Aishwarya Narayanan, Sunali Patel, Sabine Peiffer, Silvia Rüberg, Gary P. Schroth, Dave Schuster, Jonathan M. Shaffer, Elliot J Shelton, Scott Silveria, Umberto Ulmanella, Vamsi Veeramachaneni, Frank Staedtler, Thomas Peters, Toumy Guettouche, Jo Vandesompele,
Nat. Methods 11, 809–815 (2014); published online 29 June 2014; corrected after print 30 July 2014 In the version of this article initially published, the author Linda Wong was omitted from the author list. The error has been corrected in the HTML and PDF versions of the article.
Tópico(s): MicroRNA in disease regulation
2014 - Nature Portfolio | Nature Methods