P M Gamboa, Óscar Cáceres, I. Antépara, Rosa Sánchez‐Monge, Oussama Ahrazem, Gabriel Salcedo, Domingo Barber, M. Lombardero, M L Sánz,
Background: Peach allergy has two different patterns: central Europe with oral allergy syndrome (OAS) related to a primary sensitization to birch pollen Bet v 1 and profilins and southern Europe with mostly systemic symptoms, in many cases due to sensitization to lipid‐transfer proteins. Methods: Thirty peach‐allergic patients with positive skin and food challenge tests and 29 control subjects were included. Skin prick tests (SPT) with inhalant allergens, commercial peach and apple extracts and native ...
Tópico(s): Contact Dermatitis and Allergies
2007 - Wiley | Allergy
Ricardo Lima, Romualdo Salcedo, Domingos Barbosa,
A methodology to improve the efficiency of stochastic methods applied to the optimization of chemical processes with a large number of equality constraints is presented. The methodology is based on two steps: (a) the optimization of the simulation step, which involves the optimum choice of design variables and subsystems to be simultaneously solved; (b) the optimization of the nonlinear programming (NLP) problem using stochastic methods. For the first step a flexible tool (SIMOP) is used, whereby different ...
Tópico(s): Advanced Multi-Objective Optimization Algorithms
2005 - Elsevier BV | Chemical Engineering Science
M. Lombardero, F J García-Sellés, F. Polo, L. Jimeno, María José Argüeso Chamorro, Gloria García‐Casado, R. Sanchez-Monge, A. Dìaz‐Perales, Gabriel Salcedo, Domingo Barber,
Background Artemisia vulgaris is a widespread weed in the Mediterranean area and several allergens have been detected in its pollen. One of them, Art v 3, belongs to the lipid-transfer protein (LTP) family and its prevalence in Artemisia-sensitized patients or its relationship with other LTP allergens is not clear. Objective To assess the pattern of sensitization to an array of mugwort allergens in a Mediterranean population, and to study the cross-reactivity of Art v 3 with Pru p 3 and Par j 1, ...
Tópico(s): Food Allergy and Anaphylaxis Research
2004 - Wiley | Clinical & Experimental Allergy
B. Calabozo, Araceli Díaz‐Perales, Gabriel Salcedo, Domingo Barber, Florentino Polo,
The glycoprotein Pla l 1 is the major allergen from English plantain (Plantago lanceolata) pollen, which is a common cause of pollinosis in temperate areas. Three complete cDNAs for Pla l 1 isoforms were isolated by PCR using specific 3′ and 5′ primers. All three Pla l 1 cDNAs code for a 25-residue leader peptide and a 131-residue mature protein that contains two polymorphic positions, an N-glycosylation site at position 107 and six cysteine residues involved in three disulphide bridges. The allergen ...
Tópico(s): Mycotoxins in Agriculture and Food
2003 - Portland Press | Biochemical Journal
Oscar Duffort, Florentino Polo, M. Lombardero, Araceli Díaz‐Perales, Rosa Sánchez‐Monge, Gloria García‐Casado, Gabriel Salcedo, Domingo Barber,
Pru p 3 is a lipid transfer protein (LTP) that has been identified as the major peach (Prunus persica) allergen. However, little is known about the amount present in both raw and processed foodstuffs. Moreover, the in vivo release upon consumption of peach-containing foods remains unclear. We have developed a sensitive monoclonal antibody-based ELISA for Pru p 3. The method has been applied to measure the allergen levels in foodstuffs and the allergen release under different physiological conditions. ...
Tópico(s): Contact Dermatitis and Allergies
2002 - American Chemical Society | Journal of Agricultural and Food Chemistry
Produção Nacional
Revisado por pares
M. Elias-Miró, M. Mendes-Braz, Rubén Cereijo, Francesc Villarroya, Mónica B. Jiménez‐Castro, Jordi Gracia‐Sancho, Sergi Guixé‐Muntet, Marta Massip‐Salcedo, Joan Carles Domingo, Raquel Bermudo, Juan Rodés, Carmen Peralta,
This study examined whether the regulation of resistin and visfatin could reduce damage and improve regeneration in both steatotic and non-steatotic livers undergoing partial hepatectomy under ischemia-reperfusion, a procedure commonly applied in clinical practice to reduce bleeding.Resistin and visfatin were pharmacologically modulated in lean and obese animals undergoing partial hepatectomy under ischemia-reperfusion.No evident role for these adipocytokines was observed in non-steatotic livers. ...
Tópico(s): Liver Disease Diagnosis and Treatment
2013 - Elsevier BV | Journal of Hepatology
Gabriel Salcedo, Rosa Sánchez‐Monge, Domingo Barber, Araceli Díaz‐Perales,
Plant non-specific LTPs (lipid transfer proteins) form a protein family of basic polypeptides of 9 kDa ubiquitously distributed throughout the plant kingdom. The members of this family are located extracellularly, usually associated with plant cell walls, and possess a broad lipid-binding specificity closely related to their three-dimensional structure. The nsLTP fold is characterized by a compact domain composed of 4 α-helices, firmly held by a network of 4 conserved disulphide bridges. This fold ...
Tópico(s): Contact Dermatitis and Allergies
2007 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
Lydia Zapatero, M. Martínez, E. Díez-Alonso, Gabriel Salcedo, R. Sánchez‐Monge, Domingo Barber, M. Lombardero,
AllergyVolume 58, Issue 9 p. 956-956 Oral wheat flour anaphylaxis related to wheat α-amylase inhibitor subunits CM3 and CM16 Wheat α-amylase inhibitor subunits related to food allergy L. Zapatero, L. Zapatero R&D Department ALK-Abello, S.A. Miguel Fleta, 19 28037 Madrid Spain Tel: 34 9132 76100 Fax: 34 9132 76117 E-mail: [email protected]Search for more papers by this authorM. I. Martínez, M. I. Martínez R&D Department ALK-Abello, S.A. Miguel Fleta, 19 28037 Madrid Spain Tel: 34 9132 76100 Fax: 34 ...
Tópico(s): Food Allergy and Anaphylaxis Research
2003 - Wiley | Allergy
Santiago Pérez‐Tarazona, Santiago Rueda Esteban, Maria Luz García‐García, Maria Arroyas Sanchez, Inés de Mir Messa, Tatiana Acevedo Valarezo, Orlando Mesa Medina, Alicia Callejón Callejón, Elisa María Canino Calderín, Salome Albi Rodriguez, Roser Ayats, Antonio Salcedo Posadas, J. Colomer, X. Domingo Miró, Montserrat Berrocal Castañeda, Ana Villares Porto‐Dominguez,
Long-term respiratory consequences of bronchopulmonary dysplasia (BPD) in preterm infants born in the post-surfactant era ("new" BPD) remain partially unknown. The present study aimed to evaluate the respiratory outcomes of "new" BPD in adolescents who were born preterm.This multicenter, cross-sectional study included 286 adolescents born between 2003 and 2005 (mean age: 14.2 years); among them, 184 and 102 were born extremely preterm (EP; <28 weeks' gestation) and moderate-late preterm (32 to <37 weeks' ...
Tópico(s): Chronic Obstructive Pulmonary Disease (COPD) Research
2020 - Wiley | Pediatric Pulmonology
Margarida Fonseca Cardoso, Romualdo Salcedo, S. Feyo de Azevedo, Domingos Barbosa,
Tópico(s): Advanced Multi-Objective Optimization Algorithms
2000 - Elsevier BV | Chemical Engineering Science
Margarida Fonseca Cardoso, Romualdo Salcedo, S. Feyo de Azevedo, Domingos Barbosa,
An algorithm (M-SIMPSA) suitable for the optimization of mixed integer non-linear programming (MINLP) problems is presented. A recently proposed continuous non-linear solver (SIMPSA) is used to update the continuous parameters, and the Metropolis algorithm is used to update the complete solution vector of decision variables. The M-SIMPSA algorithm, which does not require feasible initial points or any problem decomposition, was tested with several functions published in the literature, and results ...
Tópico(s): Advanced Optimization Algorithms Research
1997 - Elsevier BV | Computers & Chemical Engineering
María Belén Martín-Sanz, Rosa María Salazar-de-la-Guerra, Juan Nicolás Cuenca‐Zaldívar, María Salcedo‐Pérez‐Juana, Cristina García‐Bravo, Domingo Palacios‐Ceña,
Person-centred care (PCC) has considerable effects on the clinical practice of health professionals. The purpose of this study was to describe the perspectives and perceived barriers and enablers of individuals with stroke regarding the PCC model in stroke rehabilitation.
Tópico(s): Mental Health and Psychiatry
2022 - Informa | Annals of Medicine
Leticia Tordesillas, Luis F. Pacios, Arancha Palacín, Santiago Quirce, Alicia Armentia, Domingo Barber, Gabriel Salcedo, Araceli Díaz‐Perales,
Peach non-specific lipid transfer protein (Pru p 3; nsLTP) has been characterized as the major food allergen in the adult Mediterranean population. Its wheat homologous protein, Tri a 14 has a relevant inhalant allergen in occupational baker's asthma. Different sensitization patterns to these allergens have been found in patients with this latter disorder. The objective of the present study was to characterize IgE epitopes of Tri a 14 and to compare them with those of Pru p 3 using three complementary ...
Tópico(s): Contact Dermatitis and Allergies
2009 - Elsevier BV | Molecular Immunology
Oussama Ahrazem, L. Jimeno, Gema López‐Torrejón, M. Herrero, J.L. Espada, Rosa Sánchez‐Monge, Oscar Duffort, Domingo Barber, Gabriel Salcedo,
Background The lipid transfer protein Pru p 3 has been identified as a major peach fruit allergen. However, the putative peach member of the Bet v 1 family, Pru p 1, has been neither identified nor characterized. Objectives To determine the distribution and solubility properties of the main peach allergens and to quantify Pru p 3 and Pru p 1 levels in peach and nectarine cultivars. Methods Peach peel and pulp were extracted using different buffers, and extracts were analyzed by means of sodium dodecyl ...
Tópico(s): Allergic Rhinitis and Sensitization
2007 - Elsevier BV | Annals of Allergy Asthma & Immunology
M L Sánz, M. C. García‐Avilés, Ana I. Tabar, Marta Anda, Blanca E. García, Domingo Barber, Gabriel Salcedo, Hans‐Peter Rihs, Monika Raulf,
There are no documented studies that describe natural rubber latex (NRL) sensitization in children with a history of surgical intervention but without any congenital malformation (urogenital anomalies, spina bifida, etc.), although some authors have studied NRL allergy in children without a history of surgical intervention. The aim of this work was to evaluate the sensitization profile to single NRL allergens in children without spina bifida and without repeated surgical interventions, by using different ...
Tópico(s): Food Allergy and Anaphylaxis Research
2006 - Wiley | Pediatric Allergy and Immunology
Gema López‐Torrejón, M. D. Ibáñez, Oussama Ahrazem, Rosa Sánchez‐Monge, J. Sastre, M. Lombardero, Domingo Barber, Gabriel Salcedo,
Orange is among the most widely consumed fruits, and among the plant food sources causing allergic reactions according to popular perception. However, its relevant allergenic components are virtually unknown. Profilin is a well-defined minor plant panallergen, showing prevalences around 30% in fruits and vegetables.Twenty-three orange-allergic patients were studied. Natural orange profilin, named Cit s 2, was purified by affinity chromatography and characterized by N-terminal amino acid sequencing, ...
Tópico(s): Contact Dermatitis and Allergies
2005 - Wiley | Allergy
Oussama Ahrazem, María Dolores Ibáñez, Gema López‐Torrejón, Rosa Sánchez‐Monge, J. Sastre, M. Lombardero, Domingo Barber, Gabriel Salcedo,
Lipid transfer proteins (LTPs) are relevant fruit allergens, recently proposed as model plant food allergens. No citrus fruit allergen has been characterized to date. We sought to identify and isolate citrus fruit LTPs and to explore their relevance in orange allergy.Twenty-seven patients, showing mainly oral allergy syndrome after orange ingestion, as well as positive prick responses and serum-specific IgE levels to orange, were selected. Natural orange and lemon LTPs, as well as a recombinant orange ...
Tópico(s): Contact Dermatitis and Allergies
2005 - Karger Publishers | International Archives of Allergy and Immunology
Oussama Ahrazem, María Dolores Ibáñez, Gema López‐Torrejón, Rosa Sánchez‐Monge, J. Sastre, M. Lombardero, Domingo Barber, Gabriel Salcedo,
Orange allergens are virtually unknown, in spite of the large consumption of this fruit. Germin-like proteins, together with vicilins and legumins, form the cupin superfamily of plant proteins, which includes many seed allergens.Twenty-nine patients with allergy to oranges were studied. A major IgE-binding protein from orange extracts was isolated by means of a two-step cation-exchange chromatographic protocol. The allergen was characterized by N-terminal amino acid sequencing and MALDI analysis, ...
Tópico(s): Contact Dermatitis and Allergies
2005 - Karger Publishers | International Archives of Allergy and Immunology
Ernesto Enrique, Oussama Ahrazem, Joan Bartra, M. Dolores Latorre, José Vicente Castelló, José Antonio de Mateo, Erika Montoya, Tamim Malek, Domingo Barber, Gabriel Salcedo,
To the Editor: In spite of the fact that rice is one of the most consumed cereals worldwide, few cases of true rice allergy have been reported. Two different routes of exposure leading to immediate hypersensitivity reactions have been documented: ingestion of the cooked cereal1Nakase M. Usui Y. Alvarez-Nakase A.M. Adachi T. Urisu A. Nakamura R. et al.Cereal allergens: rice-seed allergens with structural similarity to wheat and barley allergens.Allergy. 1998; 53: 55-57Crossref PubMed Scopus (26) Google ...
Tópico(s): Contact Dermatitis and Allergies
2005 - Elsevier BV | Journal of Allergy and Clinical Immunology
Blanca E. García, M. Lombardero, S. Echechipía, José M. Rivera, Araceli Díaz‐Perales, Rosa Sánchez‐Monge, Domingo Barber, Gabriel Salcedo, A. I. Tabar,
Summary Background Several lipid‐transfer proteins (LTPs) have been identified as important food allergens, especially in fruits of the Rosaceae family. The major peach ( Prunus persica ) allergen has been identified, sequenced and designated Pru p 3. Objective To present Pru p 3 as an aeroallergen able to induce occupational asthma. Methods A thorough investigation was performed in a fruit grower with occupational asthma. Skin prick–prick tests with peach leaves and prick tests with perennial respiratory ...
Tópico(s): Contact Dermatitis and Allergies
2004 - Wiley | Clinical & Experimental Allergy
Araceli Díaz‐Perales, M L Sánz, Gloria García‐Casado, Rosa Sánchez‐Monge, F J García-Sellés, M. Lombardero, Florentino Polo, P M Gamboa, Domingo Barber, Gabriel Salcedo,
Background: The peach lipid transfer protein Pru p 3 has been identified as a major allergen from this fruit. Homologous cross-reactive allergens have been found in several plant foods and pollens. Recombinant Pru p 3 has been recently produced in the yeast Pichia pastoris . Objective: We sought to evaluate the potential role of recombinant Pru p 3 as a novel tool for the diagnosis of fruit allergy. Methods: Circular dichroism analysis was used to compare the protein folding of natural Pru p 3 and ...
Tópico(s): Transgenic Plants and Applications
2003 - Elsevier BV | Journal of Allergy and Clinical Immunology
F J García-Sellés, Araceli Díaz‐Perales, Rosa Sánchez‐Monge, Manuel Alcántara Villar, M. Lombardero, Domingo Barber, Gabriel Salcedo, Montserrat Fernández‐Rivas,
<i>Background:</i> Lipid transfer proteins (LTPs) are major allergens of Rosaceae fruits in the Mediterranean area. IgE-cross-reactivity has been demonstrated in vitro among LTPs from peach, apple, chestnut and <i>Artemisia</i> pollen. The aim of this study was to evaluate the reactivity to LTPs from peach, apple, chestnut and <i>Artemisia</i> pollen by means of skin prick tests (SPTs). <i>Methods:</i> Forty-seven patients allergic to peach (peach group), 20 patients ...
Tópico(s): Allergic Rhinitis and Sensitization
2002 - Karger Publishers | International Archives of Allergy and Immunology
Araceli Díaz‐Perales, Gloria García‐Casado, Rosa Sánchez‐Monge, F J García-Sellés, Domingo Barber, Gabriel Salcedo,
Background Lipid‐transfer proteins (LTPs) have been identified as major allergens of Rosaceae fruits in populations living in areas virtually free of Fagales trees, such as several Mediterranean communities. Pru p 3 and Mal d 3, the allergens from peach and apple, respectively, have a main clinical relevance in these areas. Objetive To isolate and characterize cDNAs for Pru p 3 and Mal d 3,and to produce recombinant Pru p 3 in the yeast Pichia pastoris. Methods cDNAs for both allergens were isolated ...
Tópico(s): Contact Dermatitis and Allergies
2002 - Wiley | Clinical & Experimental Allergy
Araceli Díaz‐Perales, Ana I. Tabar, Rosa Sánchez‐Monge, Blanca E. García, Belén Gómez, Domingo Barber, Gabriel Salcedo,
Tópico(s): Contact Dermatitis and Allergies
2002 - Elsevier BV | Journal of Allergy and Clinical Immunology
Araceli Díaz‐Perales, M. Lombardero, Rosa Sánchez‐Monge, F J García-Sellés, María Pernas, Montserrat Fernández‐Rivas, Domingo Barber, Gabriel Salcedo,
Background Lipid‐transfer proteins (LTPs), but not Bet v 1 homologues, have been identified as major allergens of apple and peach in the Rosaceae fruit‐allergic population in the Mediterranean area. Many of these patients show cosensitization to mugwort pollen. LTPs have an ubiquitous distribution in tissues of many plant species, and have been proposed as a novel type of plant panallergens. Objective We sought to isolate LTPs from Artemisia pollen and from a plant food not belonging to the Rosaceae ...
Tópico(s): Contact Dermatitis and Allergies
2000 - Wiley | Clinical & Experimental Allergy
Rosa Sánchez‐Monge, M. Lombardero, F J García-Sellés, Domingo Barber, Gabriel Salcedo,
Abstract Background: Allergy to apple and Prunus fruits is frequently associated with birch pollinosis, with the principal cross-reacting allergens involved being members of the Bet v 1 family. However, a major 13-kd component, nonimmunologically related to Bet v 1, has been implicated as allergen in patients allergic to Prunoideae fruit but not to birch pollen. Objective: We sought to isolate and characterize the 13-kd allergen present in apple and peach. Methods: Sera from patients allergic to both fruits ...
Tópico(s): Allergic Rhinitis and Sensitization
1999 - Elsevier BV | Journal of Allergy and Clinical Immunology
Alicia Armentia, R. Sánchez‐Monge, Luis Gómez, Domingo Barber, Gabriel Salcedo,
Eleven purified members of the alpha-amylase/trypsin inhibitor family from wheat and barley that showed very different IgE-binding capacities when previously assayed in vitro, were used in double blind in vivo diagnostic tests to further evaluate their allergenic activity. These tests were carried out in 31 patients who showed allergic sensitization to wheat flour as verified by skin test, RAST and challenge test. The three members of the protein family with highest IgE binding in vitro (the glycosylated ...
Tópico(s): Food Allergy and Anaphylaxis Research
1993 - Wiley | Clinical & Experimental Allergy
A. M. Lázaro, Domingo Barber, Gabriel Salcedo, Enrique Méndez, Francisco Garcı́a-Olmedo,
The CM proteins are a group of major salt‐soluble endosperm proteins encoded by a disperse multigene family. The effects of high‐lysine mutations on the net accumulation in barley endosperm of three members of this group (CMa, CMb, and CMe) have been investigated. Genes CMa, CMb and CMe are located in chromosomes 1, 4, and 3 respectively. Protein CMe has been found to be identical with a previously described trypsin inhibitor. The three proteins have been quantified in the different genetic stocks ...
Tópico(s): Legume Nitrogen Fixing Symbiosis
1985 - Wiley | European Journal of Biochemistry
A. Palacín, Leticia Tordesillas, P. Gamboa, Rosa Sánchez‐Monge, Javier Cuesta‐Herranz, M L Sánz, Domingo Barber, Gabriel Salcedo, Araceli Díaz‐Perales,
Summary Background Peach is the most important fruit related to food allergy in the Mediterranean area. Pru p 3, its lipid transfer protein, has been described as the principal allergen responsible for cross‐reactivities with other foods and pollen and the severity of clinical symptoms. However, the involvement of other allergenic families cannot be ruled out. Thaumatin‐like proteins (TLPs) have been described as food allergen in several fruits, such as apple, cherry, kiwi and banana, and pollen. ...
Tópico(s): Contact Dermatitis and Allergies
2010 - Wiley | Clinical & Experimental Allergy
Ernesto Enrique, Tamim Malek, José Antonio de Mateo, JoséVicenté Castelló, M. Lombardero, Domingo Barber, Gabriel Salcedo,
Hypersensitivity to onion has been described as a cause of contact dermatitis, rhinoconjunctivitis, and asthma induced by handling of onions, but few publications in the literature report allergic reactions due to onion ingestion despite its wide use. 1 Asero R Mistrello G Roncarolo D Amato S A case of onion allergy. J Allergy Clin Immunol. 2001; 108: 309-310 PubMed Google Scholar Herein, we describe a case of a 19-year-old atopic woman who experienced urticaria after ingestion of raw onions. The ...
Tópico(s): Contact Dermatitis and Allergies
2007 - Elsevier BV | Annals of Allergy Asthma & Immunology