Victoria McKee, Frances Gibb, Legal Affairs Correspondent, Chris Petit, Patricia Davies, R. V. Milne-Day, Simon Barnes, Richard Thomson Banking Correspondent, Stephen Leather, Andrew Morgan, Gordon Allan, Pamela Stewart, William Jowett, Ronald Faux, Cliff Feltham, Sheridan Morley, Tom Lubbock, Michael Seely, Racing Correspondent, Nigel H. Harris, Sally Brompton, Walter J. Jeffrey, Philip Howard, John Bell, City Editor, Geoffrey Holroyde, George Robertson, Michael Binyon, Jeremy Kingston, Richard Evans, Media Editor, Carol Leonard, Harry Eyres, L. N. Agate, John Best, Bernard Levin, Henry Gee, John Watson, Mitchell Platts, Golf Correspondent, Harry Golombek, Sam Kiley Universities Reporter, Norman Hammond, Archaeology Correspondent, Ruth Gledhill, Peter Ball, John Spicer, Pearce Wright Science Editor, Rodney Cowton, Transport Correspondent, Robin Young, Rosemary Unsworth, J. P., Elaine Feinstein, Michael Clark, Shona Crawford Poole, Travel Editor, Peter Nichols, Andrew McEwen and Philip Webster, John Percival, Richard Thomson, Banking Correspondent, John Hurrell, John Hennessy, Charles Knevitt Architecture Correspondent, John Young, Agriculture Correspondent, Nicholas Serpell, Wolfgang M?nchau, Andrew McEwen and Andrew Buckoke, Harvey Elliott, Air Correspondent, Andrew Hislop, George Rae, John Woodcock, Neil Kelly, Stephen Johnson, Sydney Friskin, Nicholas Harling, Philip Webster, Chief Political Correspondent, Harvey Elliott, Kerry Gill and Martin Fletcher, (Michael Phillips), Brian Rynn, Kerry Gill, David Robinson, Peter Davalle, Dennis Shaw, Alan Lee, Cricket Correspondent, Rodney Cowton Transport Correspondent, Peter Dear and Jane Rackham, Paul Griffiths, Steve Acteson, Andrew McEwen, Diplomatic Correspondent, Alexandra Jackson, Peter Bryan, Roland Rudd, Beryl Dixon, Conrad Voss Bark, Christine Plant, Craig Seton, T. W. Stone, Richard Evans Media Editor, P. V. Jones, K. C. Sidwell, Colin Wallace, Barry Pickthall, Martin Searby, Ronald Butt, C. Glynne Jones, Jill Sherman and Emma Wilkins, Sonny Ramphal, R. V. Smith, Robert Lowe, David Brewerton, Christopher Thomas, John Goodbody, Kenneth Kirkwood, Graham Searjeant Financial Editor, Patrick O'Hanlon, Alan Franks, M. A. M. S. Leigh, Michael Chater, Jonathan Rendall, Alan Lee, Philip Webster and Jill Sherman, K. Haughton, Barry Wood, Michael Dynes, Donald Tebbit, Martin Waller, Michael Horsnell, Hugo Vickers, Jonathan Meades, John Young Agriculture Correspondent, Dr Thomas Stuttaford, Paul Monyard, Robert Fisk, James Spicer, Chris Than, Pearce Wright, Science Editor, Colin Campbell, Jan Raath, Philip Jacobson, Lewis Golden, Michael Harman, Michael Molyneux, Roy McKelvie,
... Heart Foundation Times Newspapers Ltd Welsh in a melo' mood Television Straying from the true Moscow Classical ... refusal Yachting: Disappointment for Australia and Rebuff for De Savary Javer loses her edge in confidence Tennis ...
1988 - Gale Group | TDA
Francisco J.P. Costa, Micaela Nave, Rita Lima‐Sousa, Cátia G. Alves, Bruna L. Melo, Ilídio J. Correia, Duarte de Melo‐Diogo,
Nano-sized materials have been widely explored in the biomedicine field, especially due to their ability to encapsulate drugs intended to be delivered to cancer cells. However, systemically administered nanomaterials face several barriers that can hinder their tumor-homing capacity. In this way, researchers are now focusing their efforts in developing technologies that can deliver the nanoparticles directly into the tumor tissue. Particularly, hydrogels assembled using Thiol-Maleimide Michael type ...
Tópico(s): Nanoplatforms for cancer theranostics
2023 - Elsevier BV | International Journal of Pharmaceutics
Produção Nacional
Revisado por pares
Hugo M. Lisboa, Matheus Augusto de Bittencourt Pasquali, Antonia Isabelly dos Anjos, Ana Maria Sarinho, Elói Duarte de Mélo, Rogério Andrade, Leonardo Batista, Janaina Lima, Yasmin Diniz, Amanda Barros,
Innovative and sustainable food preservation techniques are vital for enhancing food quality, safety, and reducing environmental impact. In this review, the methods aligned with sustainability goals are explored, focusing on their mechanisms, applications, and environmental benefits. It examines non-thermal technologies such as cold plasma, pulsed light technology, high-pressure processing (HPP), pulsed electric fields (PEFs), and ultraviolet (UV) radiation, which effectively inactivate microbes while ...
Tópico(s): Advanced Chemical Sensor Technologies
2024 - Multidisciplinary Digital Publishing Institute | Sustainability
Cátia G. Alves, Rita Lima‐Sousa, Bruna L. Melo, André F. Moreira, Ilídio J. Correia, Duarte de Melo‐Diogo,
The development of strategies capable of eliminating metastasized cancer cells and preventing tumor recurrence is an exciting and extremely important area of research. In this regard, therapeutic approaches that explore the synergies between nanomaterial-mediated phototherapies and immunostimulants/immune checkpoint inhibitors have been yielding remarkable results in pre-clinical cancer models. These nanomaterials can accumulate in tumors and trigger, after irradiation of the primary tumor with ...
Tópico(s): Cancer Research and Treatments
2022 - Multidisciplinary Digital Publishing Institute | Pharmaceutics
Ivo J. Sabino, Rita Lima‐Sousa, Cátia G. Alves, Bruna L. Melo, André F. Moreira, Ilídio J. Correia, Duarte de Melo‐Diogo,
Chemo-photothermal therapy (chemo-PTT) mediated by nanomaterials holds a great potential for cancer treatment. However, the tumor uptake of the systemically administered nanomaterials was recently found to be below 1%. To address this limitation, the development of injectable tridimensional polymeric matrices capable of delivering nanomaterials directly into the tumor site appears to be a promising approach. In this work, an injectable in situ forming ionotropically crosslinked chitosan-based hydrogel ...
Tópico(s): Nanoparticle-Based Drug Delivery
2021 - Elsevier BV | International Journal of Pharmaceutics
Rita Lima‐Sousa, Cátia G. Alves, Bruna L. Melo, André F. Moreira, António Mendonça, Ilídio J. Correia, Duarte de Melo‐Diogo,
Tópico(s): Nanoparticle-Based Drug Delivery
2021 - Elsevier BV | Materials Science and Engineering C
Rita Lima‐Sousa, Bruna L. Melo, Cátia G. Alves, André F. Moreira, António Mendonça, Ilídio J. Correia, Duarte de Melo‐Diogo,
Abstract The pursuit of effective treatments for metastatic cancer is still one of the most intensive areas of research in the biomedical field. In a not‐so‐distant past, the scientific community has witnessed the rise of immunotherapy based on immune checkpoint inhibitors (ICIs). This therapeutic modality intends to abolish immunosuppressive interactions, re‐establishing T cell responses against metastasized cancer cells. Despite the initial enthusiasm, the ICIs were later found to be associated ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2021 - Wiley | Advanced Functional Materials
Bruna L. Melo, Rita Lima‐Sousa, Cátia G. Alves, André F. Moreira, Ilídio J. Correia, Duarte de Melo‐Diogo,
Strategies combining nanomaterials’ chemotherapy and photothermal therapy hold an enormous potential for improving cancer treatment. Still, the translation of this modality has been hindered by the immunogenicity triggered by some of the polymers used for coating nanomaterials as well as by the nanostructures’ poor tumor uptake after systemic administration. To address this bottleneck, the formulation of injectable polymeric matrices capable of delivering/confining chemotherapeutics and nanomaterials ...
Tópico(s): Nanoplatforms for cancer theranostics
2022 - Elsevier BV | Biochemical Engineering Journal
Rita Lima‐Sousa, Cátia G. Alves, Bruna L. Melo, Francisco J.P. Costa, Micaela Nave, André F. Moreira, António Mendonça, Ilídio J. Correia, Duarte de Melo‐Diogo,
Injectable hydrogels intended for cancer combinatorial-photothermal therapy bring forward the possibility of attaining multifunctional systems for an improved and selective treatment for this disease.
Tópico(s): Extracellular vesicles in disease
2023 - Royal Society of Chemistry | Biomaterials Science
Andreia Granja, Rita Lima‐Sousa, Cátia G. Alves, Duarte de Melo‐Diogo, Cláudia Nunes, C. T. Sousa, Ilídio J. Correia, Salette Reis,
Photothermal therapy has emerged as a new promising strategy for the management of cancer, either alone or combined with other therapeutics, such as chemotherapy. The use of nanoparticles for multimodal therapy can improve treatment performance and reduce drug doses and associated side effects. Here we propose the development of a novel multifunctional nanosystem based on solid lipid nanoparticles co-loaded with gold nanorods and mitoxantrone and functionalized with folic acid for dual photothermal ...
Tópico(s): Extracellular vesicles in disease
2023 - Elsevier BV | Biomaterials Advances
Ariana S.C. Gonçalves, Carolina F. Rodrigues, Natanael Fernandes, Duarte de Melo‐Diogo, Paula Ferreira, André F. Moreira, Ilídio J. Correia,
Abstract Gold core silica shell (AuMSS) nanorods present excellent physicochemical properties that allow their application as photothermal and drug delivery agents. Herein, AuMSS nanorods were dual‐functionalized with Polyethylene glycol methyl ether (PEG‐CH 3 ) and Gelatin (GEL) to enhance both the colloidal stability and uptake by HeLa cancer cells. Additionally, the AuMSS nanorods were combined for the first time with IR780 (a heptamethine cyanine molecule) and its photothermal and photodynamic capacities ...
Tópico(s): Nanoparticle-Based Drug Delivery
2021 - Wiley | Biotechnology and Bioengineering
Mariana F.P. Graça, Duarte de Melo‐Diogo, Ilídio J. Correia, André F. Moreira,
Despite all the efforts that have been done up to now, the currently available wound dressings are still unable to fully re-establish all the structural and functional properties of the native skin. To overcome this situation, researchers from the tissue engineering area have been developing new wound dressings (hydrogels, films, sponges, membranes) aiming to mimic all the features of native skin. Among them, asymmetric membranes emerged as a promising solution since they reproduce both epidermal ...
Tópico(s): Silk-based biomaterials and applications
2021 - Multidisciplinary Digital Publishing Institute | Pharmaceutics
Andreia Granja, Rita Lima‐Sousa, Cátia G. Alves, Duarte de Melo‐Diogo, Marina Pinheiro, C. T. Sousa, Ilídio J. Correia, Salette Reis,
Breast cancer is the leading cause of cancer-related deaths among women worldwide. The conventional chemotherapeutic regimens used in the treatment of this disease often lead to severe side-effects and reduced efficacy. In this study, a novel drug delivery system for the chemotherapeutic drug mitoxantrone (Mito) was developed using solid lipid nanoparticles (SLN). The production of the SLN was carried out using an organic-solvent-free, low-cost method and optimized using a Box-Behnken design. SLN ...
Tópico(s): Nanoplatforms for cancer theranostics
2021 - Elsevier BV | International Journal of Pharmaceutics
Rita Lima‐Sousa, Duarte de Melo‐Diogo, Cátia G. Alves, Cátia S.D. Cabral, Sónia P. Miguel, António Mendonça, Ilídio J. Correia,
Functionalized graphene oxide (GO) and reduced GO (rGO) based nanomaterials hold a great potential for cancer photothermal therapy. However, their systemic administration has been associated with an accelerated blood clearance and/or with suboptimal tumor uptake. To address these limitations, the local delivery of GO/rGO to the tumor site by 3D matrices arises as a promising strategy. In this work, injectable chitosan-agarose in situ forming thermo-responsive hydrogels incorporating GO (thermogel-GO) ...
Tópico(s): Nanoparticle-Based Drug Delivery
2020 - Elsevier BV | Materials Science and Engineering C
Produção Nacional
Revisado por pares
Miguel M. Leitão, Cátia G. Alves, Duarte de Melo‐Diogo, Rita Lima‐Sousa, André F. Moreira, Ilídio J. Correia,
The application of Graphene Oxide (GO) in cancer photothermal therapy is hindered by its lack of colloidal stability in biologically relevant media and modest Near Infrared (NIR) absorption. In this regard, the colloidal stability of GO has been improved by functionalizing its surface with poly(ethylene glycol) (PEG), which may not be optimal due to the recent reports on PEG immunogenicity. On the other hand, the chemical reduction of GO using hydrazine hydrate has been applied to enhance its photothermal ...
Tópico(s): Nanoparticle-Based Drug Delivery
2020 - Royal Society of Chemistry | RSC Advances
Miguel M. Leitão, Duarte de Melo‐Diogo, Cátia G. Alves, Rita Lima‐Sousa, Ilídio J. Correia,
Abstract Developing technologies that allow the simultaneous diagnosis and treatment of cancer (theragnostic) has been the quest of numerous interdisciplinary research teams. In this context, nanomaterials incorporating prototypic near infrared (NIR)‐light responsive heptamethine cyanines have been showing very promising results for cancer theragnostic. The precisely engineered features of these nanomaterials endow them with the ability to achieve a high tumor accumulation, enabling a tumor's visualization ...
Tópico(s): Photodynamic Therapy Research Studies
2020 - Wiley | Advanced Healthcare Materials
Inês Mó, Ivo J. Sabino, Duarte de Melo‐Diogo, Rita Lima‐Sousa, Cátia G. Alves, Ilídio J. Correia,
The use of nanomedicines for cancer treatment holds a great potential due to their improved efficacy and safety. During the nanomedicine preclinical in vitro evaluation stage, these are mainly tested on cell culture monolayers. However, these 2D models are an unrealistic representation of the in vivo tumors, leading to an inaccurate screening of the candidate formulations. To address this problem, spheroids are emerging as an additional tool to validate the efficacy of new therapeutics due to the ...
Tópico(s): Nanoplatforms for cancer theranostics
2020 - Future Medicine | Nanomedicine
Cátia G. Alves, Duarte de Melo‐Diogo, Rita Lima‐Sousa, Ilídio J. Correia,
New insights about nanomaterials’ biodistribution revealed their ability to achieve tumor accumulation by taking advantage from the dynamic vents occurring in tumor’s vasculature. This paradigm-shift emphasizes the importance of extending nanomaterials’ blood circulation time to enhance their tumor uptake. The classic strategy to improve nanomaterials’ stability during circulation relies on their functionalization with poly(ethylene glycol). However, recent reports have been showing that PEGylated ...
Tópico(s): Photodynamic Therapy Research Studies
2020 - Elsevier BV | International Journal of Pharmaceutics
Cátia G. Alves, Duarte de Melo‐Diogo, Rita Lima‐Sousa, Elisabete C. Costa, Ilídio J. Correia,
IR780 is a near infrared (NIR) dye with a huge potential to be applied in cancer phototherapy and imaging. However, IR780 poor water solubility and acute cytotoxicity limit its direct use in cancer theragnostic. Herein, a novel Hyaluronic acid (HA)-based amphiphilic polymer was used, for the first time, in the preparation of polymeric nanoparticles (HPN) encapsulating IR780 aimed to be applied in breast cancer therapy. Furthermore, HPN co-encapsulating IR780 and Doxorubicin (DOX) were also produced ...
Tópico(s): Cancer, Hypoxia, and Metabolism
2019 - Elsevier BV | European Journal of Pharmaceutics and Biopharmaceutics
Cátia S.D. Cabral, Sónia P. Miguel, Duarte de Melo‐Diogo, P. Louro, Ilídio J. Correia,
The incorporation of reduced graphene oxide (rGO) nanomaterials into scaffolds structure can be explored to enhance the properties of these 3D matrices in bone regeneration applications. However, the weak water solubility and poor colloidal stability of rGO have hindered its incorporation in blends aimed to produce scaffolds by 3D printing. Furthermore, rGO is generally obtained by treating graphene oxide (GO) with hydrazine hydrate, which is a highly hazardous reducing agent. To overcome these problems, ...
Tópico(s): Nanoparticles: synthesis and applications
2019 - Elsevier BV | Carbon
Produção Nacional
Revisado por pares
Duarte de Melo‐Diogo, Rita Lima‐Sousa, Cátia G. Alves, Ilídio J. Correia,
Combining hyperthermia with other therapies holds a great potential for improving cancer treatment. In this approach, the increase in the body temperature can exert a therapeutic effect on cells and/or enhance the effectiveness of anticancer agents. However, the conventional methodologies available to induce hyperthermia cannot confine a high temperature increase to the tumor-site while maintaining healthy tissues unexposed and ensuring minimal invasiveness. To overcome these limitations, combination ...
Tópico(s): Carbon and Quantum Dots Applications
2019 - Royal Society of Chemistry | Biomaterials Science
Rita Lima‐Sousa, Duarte de Melo‐Diogo, Cátia G. Alves, Elisabete C. Costa, Paula Ferreira, P. Louro, Ilídio J. Correia,
Reduced graphene oxide (rGO) nanomaterials display promising properties for application in cancer photothermal therapy (PTT). rGO is usually obtained by treating graphene oxide (GO) with hydrazine hydrate. However, this reducing agent contributes for the low cytocompatibility exhibited by rGO. Furthermore, rGO has a low water stability and does not show selectivity towards cancer cells. Herein, rGO attained using an environmentally-friendly method was functionalized with a novel hyaluronic acid (HA)- ...
Tópico(s): Nanoparticle-Based Drug Delivery
2018 - Elsevier BV | Carbohydrate Polymers
Duarte de Melo‐Diogo, Elisabete C. Costa, Cátia G. Alves, Rita Lima‐Sousa, Paula Ferreira, P. Louro, Ilídio J. Correia,
PEGylated graphene oxide (GO) nanomaterials have been showing promising results in cancer therapy, due to their drug loading and photothermal capacities. However, the recent reports regarding the immunogenicity of poly(ethylene glycol) based coatings highlight the importance of investigating alternative materials to functionalize GO. Herein, GO derivatives were functionalized for the first time with an amphiphilic polymer based on poly(2-ethyl-2-oxazoline) and were co-loaded with doxorubicin (DOX) ...
Tópico(s): Nanoplatforms for cancer theranostics
2018 - Elsevier BV | European Journal of Pharmaceutics and Biopharmaceutics
João C. Boga, Sónia P. Miguel, Duarte de Melo‐Diogo, António Mendonça, P. Louro, Ilídio J. Correia,
The incidence of fractures and bone-related diseases like osteoporosis has been increasing due to aging of the world's population. Up to now, grafts and titanium implants have been the principal therapeutic approaches used for bone repair/regeneration. However, these types of treatment have several shortcomings, like limited availability, risk of donor-to-recipient infection and tissue morbidity. To overcome these handicaps, new 3D templates, capable of replicating the features of the native tissue, ...
Tópico(s): 3D Printing in Biomedical Research
2018 - Elsevier BV | Colloids and Surfaces B Biointerfaces
Duarte de Melo‐Diogo, Rita Lima‐Sousa, Cátia G. Alves, Elisabete C. Costa, P. Louro, Ilídio J. Correia,
Graphene family nanomaterials' (GFN) ability to interact with near-infrared light has propelled their application in cancer photothermal therapy. Furthermore, the graphitic lattice of GFN can adsorb different types of molecules, which has motivated their use in cancer drug delivery. However, the direct application of GFN in cancer therapy is severely hindered by their poor colloidal stability, sub-optimal safety, inefficient tumor uptake and non-selectivity towards cancer cells. To overcome these ...
Tópico(s): Carbon and Quantum Dots Applications
2018 - Elsevier BV | Colloids and Surfaces B Biointerfaces
Elisabete C. Costa, André F. Moreira, Duarte de Melo‐Diogo, Ilídio J. Correia,
Some fluorescence microscopy techniques, such as confocal laser scanning microscopy (CLSM), have a limited penetration depth. Consequently, the visualization and imaging of three-dimensional (3-D) cell cultures, such as spheroids, using these methods can be a significant challenge. Therefore, to improve the imaging of 3-D tissues, optical clearing methods have been optimized to render transparency to the opaque spheroids. The influence of the polyethylene glycol (PEG) molecular weight (MW) used in the ...
Tópico(s): Optical Coherence Tomography Applications
2018 - SPIE | Journal of Biomedical Optics
Elisabete C. Costa, André F. Moreira, Duarte de Melo‐Diogo, Ilídio J. Correia,
Spheroids are 3D in vitro platforms that fill the gap between the 2D cell cultures and animal models on the therapeutics development pipeline. Yet, the methods and equipment used in the in vitro assays are optimized for the analysis of cells cultured as monolayers. For instance, confocal laser scanning microscopy (CLSM) does not allow the observation of thick intact spheroids due to light penetration issues. To overcome this limitation, spheroids treatment with clearing agents started to be explored. ...
Tópico(s): Cell Image Analysis Techniques
2018 - Elsevier BV | Optics & Laser Technology
Ana S. Nunes, Elisabete C. Costa, Andreia S. Barros, Duarte de Melo‐Diogo, Ilídio J. Correia,
In vitro 3D cancer spheroids generally exhibit a drug resistance profile similar to that found in solid tumors. Due to this property, these models are an appealing for anticancer compounds screening. Nevertheless, the techniques and methods aimed for drug discovery are mostly standardized for cells cultured in 2D. The development of 2D cell culture models displaying a drug resistant profile is required to mimic the in vivo tumors, while the equipment, techniques, and methodologies established for ...
Tópico(s): Liver physiology and pathology
2018 - Wiley | Biotechnology Journal
Cátia G. Alves, Rita Lima‐Sousa, Duarte de Melo‐Diogo, P. Louro, Ilídio J. Correia,
IR780, a molecule with a strong optical absorption and emission in the near infrared (NIR) region, is receiving an increasing attention from researchers working in the area of cancer treatment and imaging. Upon irradiation with NIR light, IR780 can produce reactive oxygen species as well as increase the body temperature, thus being a promising agent for application in cancer photodynamic and photothermal therapy. However, IR780's poor water solubility, fast clearance, acute toxicity and low tumor ...
Tópico(s): Photodynamic Therapy Research Studies
2018 - Elsevier BV | International Journal of Pharmaceutics
Andreia S. Barros, Elisabete C. Costa, Ana S. Nunes, Duarte de Melo‐Diogo, Ilídio J. Correia,
The assessment of drug-combinations for pancreatic cancer treatment is usually performed in 2D cell cultures. In this study, the therapeutic effect and the synergistic potential of a particular drug-combination towards 2D and 3D cell cultures of pancreatic cancer were compared for the first time. Thus, the effect of Doxorubicin:Resveratrol (DOX:RES) combinations (at molar ratios ranging from 5:1 to 1:5) in the viability of PANC-1 cells cultured as 2D monolayers and as 3D spheroids was analyzed. The ...
Tópico(s): 3D Printing in Biomedical Research
2018 - Elsevier BV | International Journal of Pharmaceutics