Alexandru Burcea, Gina‐Oana Popa, Iulia Elena Florescu, Marilena Maereanu, Andreea Dudu, Sergiu Emil Georgescu, Marieta Costache,
Nowadays, in sturgeon’s aquaculture, there is a necessity for sex identification at early stages in order to increase the efficiency of this commercial activity. The basis for a correct identification is studying the different factors that influence the gonad development. The research has been directed towards molecular methods that have been employed with various degrees of success in identifying genes with different expression patterns between male and female sturgeons during their development ...
Tópico(s): Animal Genetics and Reproduction
2018 - Hindawi Publishing Corporation | International Journal of Genomics
Iulia Elena Florescu, Alexandru Burcea, Gina Oana Popa, Andreea Dudu, Sergiu Emil Georgescu, Mihaela Balaș, Sorina Dinescu, Sorina Nicoleta Voicu, Iulia Grecu, L. Dediu, Anca Dinischiotu, Marieta Costache,
Acipenser stellatus represents a species of great economical interest due to its roe used for caviar production. Therefore, it has been intensively captured for decades and nowadays, this species is on the verge of extinction. As a consequence, Acipenser stellatus is intensively raised in fish farms. Aquaculture is focused on optimizing the feeding regime of juveniles. The aim of this study was to investigate if Acipenser stellatus can adapt to a starvation/refeeding regime by assessing the effects ...
Tópico(s): Environmental Toxicology and Ecotoxicology
2019 - Polish Biochemical Society | Acta Biochimica Polonica
Andreea Dudu, Sergiu Emil Georgescu, Patrick Berrebi, Marieta Costache,
Sturgeons are fish species with a complex biology. They are also characterized by complex aspects including polyploidization and easiness of hybridization. As with most of the Ponto-Caspian sturgeons, the populations of Acipenser ruthenus from the Danube have declined drastically during the last decades. This is the first report on mitochondrial point heteroplasmy in the cytochrome b gene of this species. The 1141 bp sequence of the cytb gene in wild sterlet sturgeon individuals from the Lower Danube ...
Tópico(s): Identification and Quantification in Food
2012 - Brazilian Society of Genetics | Genetics and Molecular Biology
Andreea Dudu, Sergiu Emil Georgescu, Marieta Costache,
Sturgeons (Order Acipenseriformes) represent an extremely valuable natural resource that is now facing depletion. In the current study we evaluate if the traditional classification in subspecies of Acipenser gueldenstaedtii, Acipenser stellatus and Huso huso, endemic to Ponto-Caspian region is sustained by molecular analysis and if these represent Evolutionary Significant Units (ESUs) that should be managed separately in conservation programs. To examine the classification of taxonomic entities we ...
Tópico(s): Fish Ecology and Management Studies
2014 - Brazilian Society of Genetics | Genetics and Molecular Biology
... United States. Fisheries 6, 17–22, 26–27. Dudu, A.; Suciu, R.; Paraschiv, M.; Georgescu, S.E.; Costache, M.; Berrebi, P., 2011: Nuclear ...
Tópico(s): Fish Ecology and Management Studies
2013 - Wiley | Journal of Applied Ichthyology
Iulia Elena Florescu, Sergiu Emil Georgescu, Andreea Dudu, Mihaela Balaș, Sorina Nicoleta Voicu, Iulia Grecu, L. Dediu, Anca Dinischiotu, Marieta Costache,
Acipenser stellatus is a critically endangered species due to the anthropic influence. It has been intensively captured for decades because of its high economic value, its roe being used in the caviar industry. Therefore, Acipenser stellatus is intensively raised in fish farms for both conservation and economical purposes. Aquaculture aims to optimize the feeding regime of juveniles in order to improve its profitability. The purpose of this study was to investigate if Acipenser stellatus can adapt ...
Tópico(s): Physiological and biochemical adaptations
2021 - Multidisciplinary Digital Publishing Institute | Animals
Andreea Dudu, Radu Suciu, Marian Paraschiv, Sergiu Emil Georgescu, Marieta Costache, Patrick Berrebi,
Acipenseriformes are composed of 25 sturgeon species and two paddlefish species distributed exclusively in the northern hemisphere. The Danube River and the Black Sea were originally inhabited by six sturgeon species but two are extinct and only four are still reproducing currently in the Lower Danube: Huso huso, Acipenser stellatus, A. gueldenstaedtii and A. ruthenus. Sturgeon species hybridize more easily than other fish and the determination of pure species or hybrid status is important for conservation ...
Tópico(s): Fish Ecology and Management Studies
2011 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Andreea Dudu, Elena Ghiţă, Marieta Costache, Sergiu Emil Georgescu,
The current study investigates the genetic diversity of Racka sheep from Romania and its relationship with other breeds having different geographic origin. Mitochondrial markers (D-loop and cyt b gene) were sequenced in 40 unrelated individuals. A number of 12 distinct haplotypes, with haplotypes diversity (Hd) of 0.897 and nucleotide diversity (Pi) of 0.00437 was highlighted. The results were comparable with the ones from other sheep breeds and revealed that the Racka population has a good genetic ...
Tópico(s): Genetic and phenotypic traits in livestock
2016 - Elsevier BV | Small Ruminant Research
Alexandru Burcea, Iulia Elena Florescu, Andreea Dudu, Sergiu Emil Georgescu, Marieta Costache,
Abstract Due to construction of the Iron Gates dams, the Lower Danube has suffered a decrease in sturgeon populations. The dams have decreased sturgeon habitat area, which in turn has caused an overlap of reproduction areas for all sturgeon species. The ease with which sturgeon species can create hybrid offsprings gave rise to an increase in the number of hybrid sturgeon species now found in the Lower Danube area. We propose a set of molecular methods for hybrid species using DNA markers represented ...
Tópico(s): Genetic diversity and population structure
2014 - De Gruyter Open | Transylvanian Review of Systematical and Ecological Research
Mihaela Dumitru, Dan Cristian Vodnar, Elemér Simon, Georgeta Ciurescu, Mihaela Hăbeanu, Ionuț Sorescu, Sergiu Emil Georgescu, Andreea Dudu,
A total of 15 strains of lactic acid bacteria (LAB) were isolated from the broiler chicken’s gastrointestinal tract. All isolates were phenotypical and genetically identified. Among these isolates, only six were biochemical (API 50 CHL and ABIS soft) and genetically (16S rRNA sequencing) confirmed as Lactobacillus acidophilus, Limosilactobacillus fermentum, Levilactobacillus brevis, and Ligilactobacillus salivarius. Probiotic properties, including tolerance to pH (pH 2.0 and 3.0), bile salts (0.3% oxgall), ...
Tópico(s): Meat and Animal Product Quality
2021 - Multidisciplinary Digital Publishing Institute | Applied Sciences
Sergiu Emil Georgescu, Maria Adina Manea, Andreea Dudu, Marieta Costache,
The existence of the Hucul horse on Romanian territory has been documented from the very distant past; today Hucul is a unique breed that is part of the FAO Program for the Preservation of Animal Genetic Resources. We compared Hucul with several primitive European and Asiatic breeds in order to elucidate the origin of these horses. We analyzed a 683-bp mitochondrial DNA (mtDNA) D-loop fragment in a population of Hucul horses and compared the polymorphic sites with sequences from other primitive breeds, ...
Tópico(s): Meat and Animal Product Quality
2011 - Research Foundation of Ribeirão Preto | Genetics and Molecular Research
Dan Cârțu, D Mărgăritescu, Sarmis Marian Săndulescu, Tudor Bratiloveanu, Sandu Râmboiu, Marius Bică, E Georgescu, Catalin Dudu, Ştefan Pătraşcu, Silviu Bordu, Alexandru Marian Gogănău, Valeriu Șurlin, D. Marinescu,
Introduction: The management of this type of trauma has evolved considerably, especially with the introduction of interventional radiology (angiography, splenic artery embolization - SAE) but also the concept of non-operative treatment (NOT), defined as a follow-up of injuries, without surgery. Material and Method: We have established a strategy for dealing with cases of abdominal trauma with injuries by non-operative treatment (NOT). We applied NOT to 13 patients in 50 (26%), of which 8 were patients ...
Tópico(s): Gallbladder and Bile Duct Disorders
2021 - | Chirurgia
Andreea Dudu, Gina‐Oana Popa, Elena Ghiţă, Rodica Ștefania Pelmuș, Cristina Lazăr, Marieta Costache, Sergiu Emil Georgescu,
The state of the local breeds of farm animals is increasingly precarious worldwide because of the aggressive introduction of breeds with improved economical traits. The preference of the breeders for local breeds is due to their higher adaptability to the particular climate and relief conditions of the mountain areas, to the high rate of assimilation of the feeds from these regions and to their increased resistance to diseases. This study analyzes the genetic variation of the main four local Romanian ...
Tópico(s): Agriculture and Biological Studies
2020 - Copernicus Publications | Archives animal breeding/Archiv für Tierzucht
Andreea Dudu, Sergiu Emil Georgescu, Anca Dinischiotu, Marieta Costache,
The unique characteristics of sturgeons, as well as the difficulties in sampling determine the scarce knowledge about the genetics of this group of fish. The studies regarding taxonomic and phylogenetic relationships are limited, this group of fishes showing a great morphological variability due to their capacity of generating fertile hybrids with species belonging to different genera in their natural environment or in aquaculture conditions. Mitochondrial DNA represents a valuable marker in studies ...
Tópico(s): Identification and Quantification in Food
2009 - University of Bucharest | ROMANIAN BIOTECHNOLOGICAL LETTERS
Andreea Dudu, Sergiu Emil Georgescu,
Sturgeons are facing critical endangerment due to overfishing, habitat destruction, pollution and climate change. Their roe, highly prized as caviar, has driven the overexploitation, severely depleting wild populations. In recent years sturgeon aquaculture has experienced significant growth, primarily aimed at providing high-quality caviar and secondarily meat. This sector generates significant quantities of by-products, which are mainly treated as waste, being mostly discarded, impacting the environment, ...
Tópico(s): Meat and Animal Product Quality
2024 - Multidisciplinary Digital Publishing Institute | Animals
Gina‐Oana Popa, Miad Khalaf, Andreea Dudu, Angela Curtean–Bănăduc, Doru Bănăduc, Sergiu Emil Georgescu, Marieta Costache,
ABSTRACT Among the species of salmonids that exist in Romania, the most common is the brown trout (Salmo trutta, morpha fario, Linnaeus, 1758), with a high commercial potential, being used for aquaculture or fishing. Unfortunately, its natural habitat is disrupted by human activities and in order to avoid local extinction of Salmo trutta fario repopulation strategies are being applied. The repopulation activities must be carefully designed and conducted taking into consideration that there is a high ...
Tópico(s): Fish Biology and Ecology Studies
2013 - De Gruyter Open | Transylvanian Review of Systematical and Ecological Research
M. I. Georgescu, Irina Gheorghe, Andreea Dudu, Ilda Czobor Barbu, Marieta Costache, Violeta Corina Cristea, Veronica Lazăr, Mariana Carmen Chifiriuc,
This is the first report of an OXA-72-producing Acinetobacter baumannii strain in Romania, isolated from chronic leg ulcer samples. Identification of the strain was performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Presence of carbapenem resistance genes was investigated by PCR and sequencing. Our data support the spread of the bla OXA-72 gene in Eastern Europe.
Tópico(s): Vibrio bacteria research studies
2016 - Elsevier BV | New Microbes and New Infections
Gina‐Oana Popa, Andreea Dudu, Doru Bănăduc, Angela Curtean–Bănăduc, Alexandru Burcea, Dorel Ureche, Ramona Nechifor, Sergiu Emil Georgescu, Marieta Costache,
The Carpathian Mountains are one of the most complex orogenetic areas of Europe, with unique fauna, including the brown trout ( Salmo trutta ). In this study we performed population genetic analysis of 12 different S. trutta populations using two types of molecular markers: nine microsatellites and mitochondrial D-loop sequences. The following working hypothesis was considered: the Romanian Carpathians and their surrounding lowlands can be key relief units based on which the S. trutta genetic diversity, ...
Tópico(s): Genetic and phenotypic traits in livestock
2019 - EDP Sciences | Aquatic Living Resources
Sergiu Emil Georgescu, Maria Adina Manea, Andreea Dudu, Marieta Costache,
The Mangalitsa pig, a swine breed belonging to the protected gene fund of original and primitive animal breeds of the FAO (Food and Agriculture Organization), has been known to inhabit Romanian territories since the 19th century. The aim of this study was to compare the Mangalitsa breed with several European and Asiatic swine breeds in order to emphasize its uniqueness and to elucidate its origin. For this purpose, we analyzed a 613 bp mitochondrial DNA D-loop fragment and 1140 bp of the cytochrome ...
Tópico(s): Molecular Biology Techniques and Applications
2012 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Iulia Elena Florescu, Alexandru Burcea, Gina Oana Popa, Andreea Dudu, Sergiu Emil Georgescu, Marieta Costache,
Acipenser stellatus (stellate sturgeon) represents a sturgeon species of great importance for the scientific community and fishermen that has been intensively captured for decades. Besides of overfishing and poaching, their reproduction sites have been destroyed and their natural habitats were affected by pollution. In consequence, wild populations of stellate sturgeon have decreased dramatically, this species being considered critically endangered. In order to conserve this species, efforts have ...
Tópico(s): Aquaculture Nutrition and Growth
2019 - Iranian Fisheries Research Organization | Iranian journal of fisheries science/Iranian journal of fisheries science
Gina‐Oana Popa, Andreea Dudu, Doru Bănăduc, Angela Curtean–Bănăduc, Teodora Barbălată, Alexandru Burcea, Iulia Elena Florescu, Sergiu Emil Georgescu, Marieta Costache,
DNA barcoding is a molecular technique frequently used either for biodiversity monitoring and fish conservation or in the identification of the species of origin for raw and processed food from restaurants or fish markets. The most important aspect of this technique is that it works for all stages of life and can be used to distinguish between closely related taxa. Also, the technique has been used to unmask attempts of frauds in fish markets where more desirable and expensive fish are sometimes ...
Tópico(s): Genetic diversity and population structure
2017 - EDP Sciences | Aquatic Living Resources
Sergiu Emil Georgescu, Oana Canareica, Andreea Dudu, Marieta Costache,
ABSTRACT Sturgeons such as Acipenser baerii and Acipenser gueldenstaedtii are the most common species farm raised worldwide in aquaculture, because of the dwindling natural sources of caviar and meat. Also, these species can easily participate in the formation of an intraspecific hybrid with a great potential for growth in aquaculture. Microsatellites are nuclear markers consisting of short repetitive sequence, dispersed across the entire genome with characteristics such as relatively small size ...
Tópico(s): Aquaculture Nutrition and Growth
2013 - De Gruyter Open | Transylvanian Review of Systematical and Ecological Research
Andreea Dudu, Maria Samu, Marilena Maereanu, Sergiu Emil Georgescu,
The sturgeons (order Acipenseriformes) are caviar producers and some of the most valuable fish species worldwide. Due to different reasons, wild populations are now at the brink of extinction. The high demand for caviar has led to the development of aquaculture for restocking and caviar production. Since the caviar from different species has different prices depending on the quality and attempts of commercial fraud based on species substitution have been found, correct species identification is more ...
Tópico(s): Genetic diversity and population structure
2022 - Multidisciplinary Digital Publishing Institute | Foods
Mihaela Dumitru, Nicoleta Aurelia Lefter, Mihaela Hăbeanu, Georgeta Ciurescu, Dan Cristian Vodnar, Elemér Simon, Ionuț Sorescu, Sergiu Emil Georgescu, Andreea Dudu,
Fourteen lactic acid bacteria (LAB) strains were isolated from the intestinal tract and feces of piglets. Among these isolates, only twelve were biochemically (API 50 CHL and ABIS online for bacterial identification) and genetically (16S rRNA sequencing) confirmed as Limosilactobacillus fermentum, Lactobacillus acidophilus, and Lactiplantibacillus plantarum. Experiments to evaluate the probiotic potential of the isolates including pH tolerance (pH 2.0 and 3.0), bile salts (0.3% ox gall) resistance, hemolysis ...
Tópico(s): Animal Nutrition and Physiology
2023 - Multidisciplinary Digital Publishing Institute | Agriculture
Alexandru Burcea, Gina‐Oana Popa, Iulia Elena Florescu, Andreea Dudu, Sergiu Emil Georgescu, Marieta Costache,
The aim of this study is to help the conservation effort of the Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833 population from Lower Danube, ensuring its sustainable development in regards to aquaculture and wild populations.We evaluated the genetic diversity of five aquaculture strains using molecular markers, determining the suitability of employing the individuals in ex situ conservation and in restocking programs.The mitochondrial cytochrome b gene analysis highlighted that some sequences ...
Tópico(s): Genetic and phenotypic traits in livestock
2017 - Hungarian Natural History Museum | Acta Zoologica Academiae Scientiarum Hungaricae
Dana Popa, ANDREEA DUDU, Sergiu Emil Georgescu, Alexandru Burcea, R. Popa, Livia Vidu,
Romanian buffalo (Bubalus bubalis, LINNAEUS, 1758) was an important resource for meat and milk, plus a number of traditions associated with this species.Currently in our country buffalo herd is continuously decreasing.In this context, conservation of genetic resources represents an extremely important action.Loss of genetic diversity is the main cause of the disappearance of populations or their entrance in the area of vulnerable or endangered risk status.The goal of this study is to evaluate the ...
Tópico(s): Genetic diversity and population structure
2020 - University of Bucharest | ROMANIAN BIOTECHNOLOGICAL LETTERS
Sergiu Emil Georgescu, Oana Canareica, Gina Oana Popa, Andreea Dudu, Marieta Costache,
Sturgeons, a class of fish with a particular scientific and commercial importance have been overexploited through overfishing and poaching, nowadays being at the brink of extinction. The developments of the molecular techniques allow the detection of intraspecific genetic differences and could ensure an increased ability to characterize the extent of genetic variation in sturgeon populations, both aquaculture and wildlife. In our study we optimized a protocol for microsatellites amplification in ...
Tópico(s): Genetic and phenotypic traits in livestock
2013 - Agroprint Timisoara | Lucrări ştiinţifice. Zootehnie şi biotehnologii
Alexandru Burcea, Gina‐Oana Popa, Iulia Elena Florescu, Sami Gharbia, Gisela Găină, Andreea Dudu, Marilena Maereanu, Anca Hermenean, Sergiu Emil Georgescu, Marieta Costache,
The scope of this study is to investigate the expression of dmrt1 , foxl2 , ar , star and sox9 genes in the context of the gonad development stage of 21 month‐old Best Beluga individuals. No significant difference was observed between males and females but the cyp17a1 gene showed higher expression in male than in female gonads. The results suggest that during sampling the females were in perinucleolar stage and the males in early spermatogenesis stage which led to specific patterns of expression for the ...
Tópico(s): Animal Genetics and Reproduction
2018 - Wiley | Journal of Fish Biology