R. Belcher, D. F. Gibbons, T.S. West,
... of the two systems, in the presence of EDTA, at PH 4–5, are 0.12 and ... reducing powers, attempts were made to use ferrous-EDTA and cuprous-EDTA as reductimetric reagents. A variety of oxidants was ... Theoretical considerations suggest the existence of a cuprous-EDTA complex with a formation constant of ca. 1018. ... oxydation de ces deux systèmes, en présence d'EDTA, à un pH 4–5 sont respectivement 0. ... des essais ont été faits avec Fe+2-EDTA et Cu+-EDTA. En effet un grand nombre d'oxydants ont ... font supposer l'existence d'un complexe Cu+-EDTA avec une constante de formation d'environ 1018. ...
Tópico(s): Metal complexes synthesis and properties
1955 - Elsevier BV | Analytica Chimica Acta
Harry Foreman, T. T. Trujillo, O. S. Johnson, Camille Finnegan,
1. A comparison of Ca EDTA and Zr citrate for the therapy of acute Pu poisoning was made in which it was found that Ca EDTA brought forth greater Pu excretion (50% greater than ... Pu skeletal content than occurred in the Ca EDTA treated animals. Combined Ca EDTA and Zr citrate treatment produced the same results as Zr citrate alone. 2. Ca EDTA administered long after Pu was deposited in bone ... 3. A comparison of a regimen of Na EDTA and Ca EDTA with one using Ca EDTA alone in acute Pu exposures showed no advantage ...
Tópico(s): Radioactivity and Radon Measurements
1955 - SAGE Publishing | Experimental Biology and Medicine
A method for determining nickel using EDTA is described. A small excess of EDTA is added, the pH adjusted to 2.7–2.9 and the solution back-titrated ... determination of the complex-forming constant of Th-EDTA is described, based on the decrease in extinction of an Ni-EDTA solution to which Th+4 has been addd. ... du nickel au moyen de l'acide éthylèndiaminotetraacètitique (EDTA). Un léger excès de réactif est ajouté, le ... de la constante de formation du complexe Th EDTA, basée sur mit diminution de l'extinction. d' ...
Tópico(s): Analytical chemistry methods development
1956 - Elsevier BV | Analytica Chimica Acta
Shizuo WATANABE, W. W. Sleator,
The relaxing effects of EDTA on glycerol-treated muscle fibers were studied quantitatively in the presence of Mg++, Ca++, and Mn++ in various combinations. These ... were drawn: 1. All of the three substances EDTA, Mg++, and ATP must be present to produce ... well-washed fibers. 2. The effective concentration of EDTA depends upon the concentration of Mg++ present. 3. Enough Mg++ and EDTA remain on an EDTA-relaxed fiber after it has been washed up ... the place of Mg++, but Ca++ cannot. 5. EDTA relaxation can be reversed by both Ca++ and ...
Tópico(s): Pharmacological Effects and Assays
1957 - Elsevier BV | Archives of Biochemistry and Biophysics
... Aktin ändert sich nicht, wenn das aktin mit EDTA behandelt wird. 5. Kartoffelapyrase spaltet das gebundene ADP ... Phosphat. Beide Bruchstücke des ADP bleiben auch nach EDTA-Behandlung der ungelösten Proteinstrukturen fest gebunden. 6. Der ... Calciumgehalt der ungelösten Proteinstrukturen aus frischem Muskelwird durch EDTA-Waschung um 50% erniedrigt. Der Magnesiumgehalt wird durch EDTA-Behandling nicht verändert. 9. Der Calciumgehalt der ungelösten Proteinstrukturen aus totenstarrem Muskel wird durch EDTA-Behandlung nicht weiter erniedrigt. 10. Das Calcium ist ...
Tópico(s): Cardiomyopathy and Myosin Studies
1957 - Elsevier BV | Biochimica et Biophysica Acta
... the effect of a standardized infusion of calcium EDTA upon the excretion of lead in the urine ... urinary excretion of lead due to the calcium EDTA (the EDTA lead excretion) varied up to about 0·6 ... varying degrees attributable to childhood lead poisoning, the EDTA lead excretion was always above this figure, as ... disease attributable to causes other than lead, the EDTA lead excretion was comparable with that of the ... a significant correlation was also demonstrated between the EDTA lead excretion and both the plasma creatinine level ...
Tópico(s): Heavy Metal Exposure and Toxicity
1963 - Royal Australasian College of Physicians | Australasian Annals of Medicine
F Nelson, R. A. Day, Kurt A. Kraus,
... V, Cr, Mn, Co, Ni and Zn) in EDTA solutions were studied as a function of EDTA concentration, supporting electrolyte concentration (NH4Cl) and pH. Applicability ... illustrations of several separations are given. Adsorbability of EDTA by the chloride form of the exchanger was ... of loading. The selectivity of the exchanger for EDTA is sufficiently low to permit separations in EDTA solutions with the resin remaining substantially in the ... distribution coefficients of metals as a function of EDTA and chloride concentration, the charges of a number ...
Tópico(s): Environmental Chemistry and Analysis
1960 - Pergamon Press | Journal of Inorganic and Nuclear Chemistry
1. The effects of EDTA on the transmembrane potentials and mechanical events of the ventricular muscle of rabbits were studied under different ionic conditions.2. EDTA (5×10-3M) caused a relaxation and complete ... two stable states spontaneously and repeatedly.4. When EDTA was added to and NaCl was depleted from ... the duration rather shortened despite the action of EDTA. The lowering of spike height was continuous but ... into two components, spike and plateau.5. When EDTA was added and KCl was depleted from the ...
Tópico(s): Ion channel regulation and function
1964 - Physiological Society of Japan | The Japanese Journal of Physiology
Akira Hanaki, Morizo Ishidate,
Fe(III) complexes of EDTA and diethylenetriamine pentaacetic acid (DETAPAC) at low concentrations (between 1 and 100 μM) produced up to a 20-fold increase ... to half the levels seen under anaerobic conditions. EDTA alone was much less effective at stimulating indicine ... stimulating microsomal indicine N-oxide reduction. Fe(III)-EDTA stimulated indicine N-oxide reduction by purified NADPH- ... iron complexes will stimulate indicine N-oxide reduction. EDTA complexes of other transition metal ions do not ... oxide, appear to be formed during Fe(II)-EDTA-dependent reduction of indicine N-oxide under anaerobic ...
Tópico(s): Chemical Synthesis and Analysis
1962 - Elsevier BV | Biochimica et Biophysica Acta
P. Brett Hammond, Arthur L. Aronson, Walter C. Olson,
The mechanism whereby ethylenediaminetetraacetate (EDTA) acts to increase the excretion of lead was studied. All experiments were conducted on rats which received lead, 7 mg/kg, containing Pb210. The administration of EDTA resulted in marked removal of lead from the ... terminated by the end of the period of EDTA infusion. Depletion of the lead burden of soft tissues continued well past the termination of EDTA infusion. Excretion of mobilized lead occurred mainly during the period of EDTA infusion. EDTA had no effect on the ultrafilterability ...
Tópico(s): Heavy Metal Exposure and Toxicity
1967 - American Society for Pharmacology and Experimental Therapeutics | Journal of Pharmacology and Experimental Therapeutics
R. A. J. Conyers, Donald Birkett, F. C. Neale, Solomon Posen, Joan Brudenell-Woods,
The effects of EDTA on the human alkaline phosphatases (orthophosphoric monoester phosphohydrolase, EC 3.1.3.1) of bone, intestine and placenta, have ... means of automated methods employing controlled concentration gradients. EDTA has three effects of these phosphatases: (a) In the presence of excess substrate, low concentrations of EDTA (10−5-10−3 M) cause a loss ... activity which is the same irrespective of the EDTA concentration. (b) Above 10−3 M EDTA bone and intestinal phosphatases display an increasing loss ...
Tópico(s): Enzyme function and inhibition
1967 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
... between the therapeutic efficacy of sodium calciumedetate (Ca-EDTA) and penicillamine (PCA), administered intravenously and orally. The ... is discussed. With the dosages given, intravenous Ca-EDTA promoted the greatest output of lead in the ... δ-aminolaevulinic acid (ALA) in urine. Oral Ca-EDTA was found to be greatly inferior in both ... quantitatively great, whereas the oral absorption of Ca-EDTA was very slow and quantitatively small. The possible ... indications were found of resorption of the Ca-EDTA-lead complex but not of the PCA-lead ...
Tópico(s): Contact Dermatitis and Allergies
1967 - BMJ | Occupational and Environmental Medicine
D.L. Fuhrman, GEORGE W. LATIMER, Jacqueline Bishop,
Ethylenediaminetetra-acetic acid (EDTA) and nitrilotri-acetic acid (NTA) can be differentiated and determined by titration with metal ions to visual metallochromic dye end-points. EDTA can be determined without interference from NTA, either ... 70° using Tiron indicator. The total chelating power (EDTA + NTA) can be determined either by titrating with ... concentration at which NTA can be determined in EDTA by titration to the iron(III)-Tiron end- ... determined using the method of continuous variations. Äthylendiamintetraessigsäure (EDTA) und Nitrilotriessigsäure (NTA) konnen durch Titration mit Metallionen ...
Tópico(s): Analytical Chemistry and Sensors
1966 - Elsevier BV | Talanta
Ove A. Nedergaard, A Vagne, John A. Bevan,
... enhanced by the chelating agents, (ethylenedinitrilo) tetraacetic acid (EDTA), either as the disodium salt (Na 2 EDTA) or as the calcium disodium salt (CaNa 2 EDTA), and sodium pyrophosphate, but not by 2,29- ... The enhanced norepinephrine uptake seen with CaNa 2 EDTA was inhibited by 8-hythoxyquinoline but not by ... 29-bipyridine. In the absence of CaNa 2 EDTA, repeated replacement of the bath fluid with physiologic ... that seen in the presence of CaNa 2 EDTA. The removal of Ca ++ and Mg ++ either singly ... physiologic salt solution had no effect on the EDTA-enhanced norepinephrine uptake. However, in the absence of ...
Tópico(s): Cardiac electrophysiology and arrhythmias
1968 - American Society for Pharmacology and Experimental Therapeutics | Journal of Pharmacology and Experimental Therapeutics
Mutsuo Kodama, Chifumi Sasaki, Tomohiko Noda,
Abstract BT can displace EDTA-OH and GEDTA from their cobalt(II) and nickel(II) chelate as in the case of the substitution reaction of BT with cobalt(II)-EDTA chelate or nickel(II)-EDTA chelate. In this paper, kinetics of the substitution ... with cobalt(II)- and nickel(II)-chelates of EDTA-OH and of GEDTA was studied spectrophotometrically. All ... and first-order in metal(II)-chelate of EDTA-OH or GEDTA, and to proceed through two ... the substitution reaction of BT with cobalt(II)-EDTA chelate. Although EDTA-OH differs from EDTA by ...
Tópico(s): Inorganic and Organometallic Chemistry
1968 - Oxford University Press | Bulletin of the Chemical Society of Japan
... being used as masking agent. An excess of EDTA is added and the surplus EDTA is back-titrated with lead nitrate, with Xylenol ... Thiourea is then added to decompose the mercury-EDTA complex and the liberated EDTA is again back-titrated with lead nitrate. The ... comme agent dissimulant. On ajoute un excès d'EDTA et le surplus d'EDTA est titré en retour par le nitrate de ... de la thiourée pour décomposer le complexe mercure-EDTA et l'EDTA libéré est de nouveau titré en retour par ... bei dem Thioharnstoff als Maskierungsmittel verwendet wird. Überschüssige EDTA wird zugesetzt und der Überschuß mit Bleinintrat zurücktitriert, ...
Tópico(s): Electrochemical Analysis and Applications
1969 - Elsevier BV | Talanta
... 3 hours with 0.001 M ethylenediaminetetraacetic acid (EDTA) in phosphate buffer, pH 7. EDTA reduced infectivity more readily at pH 9 than ... pH 7. Magnesium ions reduced the effect of EDTA on virus infectivity. When 0.01 M MgSO4 ... present in the incubating solution, losses due to EDTA were markedly reduced. Preincubation of the virus with Mg2+ prevented infectivity losses from occurring during subsequent EDTA treatments. SBMV treated with EDTA at pH 9 sedimented as two distinct infectious ... incubated for 12 hours with 0.01 M EDTA, pH 7, and at 98 S if incubated ...
Tópico(s): Plant Disease Resistance and Genetics
1969 - Elsevier BV | Virology
Newell W. Johnson, D.F.G. Poole, John E. Tyler,
... prism centres and ethylene diamine tetra-acetic acid (EDTA), at neutral pH, preferentially removed prism peripheries. A ... by the observations that application of very dilute EDTA solutions resulted in the formation of narrow clefts ... to manifest some chelating activity, and etching with EDTA at acid pH, produced variable results with features typical of both acid and neutral EDTA attack. When the ionization of acids was inhibited ... permeability of prism junctions, which admits the large EDTA molecule and acid anions more readily than the ...
Tópico(s): Dental Erosion and Treatment
1971 - Elsevier BV | Archives of Oral Biology
... than the latter. When added to reaction mixtures, EDTA inhibited the activity of the enzyme very rapidly. ... of divalent ions or by the removal of EDTA by dialysis or gel filtration. Preincubation of the enzyme with EDTA or ATP caused an irreversible inactivation. The rate ... mentioned above. ATP was much less effective than EDTA in inactivating the enzyme. The inactivation caused by ... of exposure to ATP but the kinetics of EDTA inactivation were more complex. AMP and structurally related ... enzyme against the inhibiting effects of ATP and EDTA. There was a good correlation between the ability ...
Tópico(s): Hemoglobin structure and function
1971 - Elsevier BV | Archives of Biochemistry and Biophysics
1. Extracellular Ca and EDTA concentrations in arterial smooth muscle in Ca-free solutions with and without EDTA were estimated from (45)Ca and [(14)C]EDTA washout studies. Ca concentrations were calculated from the ... of diffusion of Ca in the tissue, while EDTA concentrations were calculated by analysing an exponential phase of [(14)C]EDTA washout and checked by separate determinations of coefficient of diffusion of EDTA in the tissue.2. Extracellular Ca concentrations after ... tissue stores of Ca were removed.3. Extracellular EDTA concentration at the centre of the tissue reached ...
Tópico(s): Cardiomyopathy and Myosin Studies
1972 - Wiley | The Journal of Physiology
... 3)H]thymidine into DNA was inhibited by EDTA and diethylenetriamine-NNN'N''N''-penta-acetate but ... of the chelators. 2. The inhibition caused by EDTA was most effectively reversed by Zn(2+) but ... 2+). Very little if any activation of the EDTA-inhibited system was obtained with Co(2+), Cu( ... Zn(2+)-activated lymphocytes in the presence of EDTA markedly increased thymidine incorporation. Addition of Cd(2+) ... occurred at high Zn(2+) concentrations. 4. If EDTA was added more than 15h after phytohaemagglutinin, the ...
Tópico(s): Seed and Plant Biochemistry
1972 - Portland Press | Biochemical Journal
... copper(II), based on decomposition of the copper-EDTA complex at pH 5–6. Among the various ... have been found most suitable. An excess of EDTA is added and the surplus EDTA is back-titrated with lead (or zinc) nitrate ... mixture is then added to decompose the copper-EDTA complex and the liberated EDTA is again back-titrated with lead (or zinc) ... untersucht. Sie beruht auf der Zersetzung des Kupfer-EDTA-Komplexes bei pH 5–6. Unter den verschiedenen ... 2, 2'-Dipyridyl) am besten. Ein Überschuß von EDTA wird zugegeben und die überschüssige EDTA mit Blei-( ...
Tópico(s): Analytical Chemistry and Sensors
1972 - Elsevier BV | Talanta
Gerald S. Yoneda, Robert A. Holwerda,
... blue copper protein stellacyanin (St(I) by Co(EDTA)-- has been performed. Observed rate constants approach a saturation limit with increasing [Co(EDTA)--] at pH 7, consistent with a mechanism involving ... Cu(I) to Co(III) electron transfer: Co(EDTA)-- + St(i Qp in equilibrium Co(EDTA)-- ---St(I) Co(EDTA)-- ---St(I) k2 leads to Co(EDTA)2-- ---St(II) (Qp = 149 M--1, k2 = ... 3+ as the oxidant. Linear kobsd VS. [Co(EDTA)--] plots are reported for the Co(EDTA)-- oxidation of cuprous stellacyanin at pH 10 (k = ... at pH 7 in the presence of excess EDTA (k = 21.2 M--1 sec--1; 25. ...
Tópico(s): Protein Interaction Studies and Fluorescence Analysis
1978 - Elsevier BV | Bioinorganic Chemistry
Issei Mabuchi, Takashi Shimizu, Yoriko Mabuchi,
... Tris · HCl (pH 7.8)-0.1 mm EDTA-0.5 mm dithiothreitol (KCl-EDTA), while with 1 mm Tris · HCl (pH 7.8)-0.1 mm EDTA-0.5 mm dithiothreitol (Tris-EDTA) around 90% of the activity was extracted. The main adenosine triphosphatase (ATPase) in the KCl-EDTA extract had a sedimentation coefficient of 20S and that in the Tris-EDTA extract had a sedimentation coefficient of 12S. The ... Recombination of the ATPase activity from the KCl-EDTA extract was almost complete while that from the Tris-EDTA extract was around 50%. Outer arms disappeared preferentially ...
Tópico(s): Polyamine Metabolism and Applications
1976 - Elsevier BV | Archives of Biochemistry and Biophysics
M.M. Jonah, E.A. Cerny, Y.E. Rahman,
Liposomes containing ethylenediaminetetraacetic acid (EDTA) were prepared with different surface properties by varying the liposomal lipid constituents. Positively charged liposomes were prepared with a mixture ... of phosphatidylcholine and cholesterol. Distributions of 14C-labeled EDTA were determined in mouse tissues from 5 min ... by the different liposomal lipid compositions. Uptake of EDTA by spleen and marrow was highest from negatively charged liposomes. Uptake of EDTA by lungs was highest from positively charged liposomes; ...
Tópico(s): Surfactants and Colloidal Systems
1975 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Biomembranes
J.W. Simmelink, V.K. Nygaard, David B. Scott,
Prepared human enamel surfaces were treated with either EDTA or dilute HCl and viewed in the SEM. ... are attacked in the same fashion by both EDTA and HCl: the core of the crystal is ... At the prism level, however, the effects of EDTA and acid differ strikingly. The attack of EDTA depended on the size of the spaces between ... at the periphery of the prism head. The EDTA created an arcuate cleft which widened with time ... maintains its gross structure. Although the effect of EDTA also fits into this system, it is limited ...
Tópico(s): Fluoride Effects and Removal
1974 - Elsevier BV | Archives of Oral Biology
H. E. Gilleland, J. D. Stinnett, R. G. Eagon,
... became resistant to the lytic effect of ethylenediametetraacetate (EDTA) when grown in a Mg 2+ -deficient medium. ... the cell wall associated with the shift to EDTA-resistance, a freeze-etch study was performed. Upon ... convex) layers. The concave cell wall layer of EDTA-sensitive cells grown in Mg 2+ -sufficient medium ... resting on an underlying smooth support layer. Upon EDTA treatment, approximately one-half of these spherical units ... in Mg 2+ -deficient medium were resistant to EDTA. The concave cell wall layer of EDTA-resistant ...
Tópico(s): Biopolymer Synthesis and Applications
1974 - American Society for Microbiology | Journal of Bacteriology
Louis R. Cantilena, Curtis D. Klaassen,
... 45 g which were given CaNa2-ethylenediaminetetraacetic acid (EDTA) (0.5 mmol/kg ip) or CaNa2EDTA plus ... 3.5 mg Cd/kg. Mice given either EDTA or EDTA + SA were protected from the lethal effects of ... survival was found between animals treated with either EDTA + SA or EDTA alone at any dosage of Cd. The tissue ... of mice also treated with various dosages of EDTA with or without a fixed dosage of salicylate (2 mmol/kg). EDTA, in dosages as low as 0.3 mmol/ ... when compared to that found in mice given EDTA alone.
Tópico(s): Immunotoxicology and immune responses
1980 - Elsevier BV | Toxicology and Applied Pharmacology
Clayton W. Naeve, Christiane M. Kolakofsky, Donald F. Summers,
... Renografin gradients in the presence or absence of EDTA. It was shown that vRNP and icRNP sediment ... in sucrose and Renografin in the absence of EDTA; however, icRNP sedimented more slowly in the presence of EDTA than did vRNP. Polyacrylamide gel electrophoresis of the proteins of vRNA and icRNP recovered from EDTA-containing gradients demonstrated that both RNP structures contained ... of both RNP structures, in the absence of EDTA, demonstrated that both exist as helical structures ∼20 by 700 nm. However, in the presence of EDTA the icRNP was completely uncoiled with a mean ...
Tópico(s): Virology and Viral Diseases
1980 - American Society for Microbiology | Journal of Virology
Birgitta Engström, Harald Norin, Mohammad Jawaid, Folke Ingman,
... with 109 Cd) alone or in combination with EDTA (ethylenediaminetetraacetic acid) in Cd: EDTA molar ratios of 1:0.4 or 1: ... kg b.wt.)or Cd in combination with EDTA was 60% and 0%, respectively. Furthermore a marked ... first 24 hours following exposure to Cd and EDTA (about 80% in the Cd + EDTA 1:4‐group and about 45% in the ... given alone or simultaneously with an excess of EDTA was bound similarly to different proteins in liver ... more of the Cd administered in the Cd + EDTA (1:0.4)‐solution was bound to high ... Cd‐exposed mice. However, cadmium given simultaneously with EDTA in the (1:4)‐ solution was not toxic. ...
Tópico(s): Pharmacological Effects and Toxicity Studies
1980 - Wiley | Acta Pharmacologica et Toxicologica