Freda Passam, Lin Lin, Srila Gopal, Jack D. Stopa, Lola Bellido‐Martín, Mingdong Huang, Barbara C. Furie, Bruce Furie,
... formation. Another thiol isomerase, endoplasmic reticulum protein 5 (ERp5), is involved in platelet activation. We show here the involvement of ERp5 in thrombus formation using the mouse laser-injury ... thrombosis and a specific antibody raised against recombinant ERp5. Anti-ERp5 antibody inhibited ERp5-dependent platelet and endothelial cell disulfide reductase activity in vitro. ERp5 release at the thrombus site was detected after infusion of Alexa Fluor 488-labeled anti-ERp5 antibody at 0.05 μg/g body weight, ...
Tópico(s): Toxin Mechanisms and Immunotoxins
2015 - Elsevier BV | Blood
Peter A. Jordan, Joanne M. Stevens, Gary P. Hubbard, Natasha Barrett, Tanya Sage, Kalwant S. Authi, Jonathan M. Gibbins,
... protein disulfide isomerase (PDI), endoplasmic reticulum protein 5 (ERP5), and ERP57. Recent evidence supports an alternative role ... identified the presence of the thiol isomerase protein ERP5. Further study showed that ERP5 is resident mainly on platelet intracellular membranes, although ... a range of platelet agonists. Blocking cell-surface ERP5 using inhibitory antibodies leads to a decrease in ... disruption of integrin function, as we observed that ERP5 becomes physically associated with the integrin β3 subunit ...
Tópico(s): Blood properties and coagulation
2004 - Elsevier BV | Blood
Chunmei Yang, Cheng Qian, Weiwei Zheng, Guanglu Dong, Shan Zhang, Feihui Wang, Zhonghong Wei, Yuhua Xu, Aiyun Wang, Yang Zhao, Yin Lu,
... determine the binding between endoplasmic reticulum protein 5 (ERp5) and GRh2. We demonstrated that GRh2 exerted prominent ... GRh2 was capable of diminishing the expression of ERp5 and GRh2 directly bound to ERp5 in MDA-MB-231 cells as well as ... able to insert into the binding pocket of ERp5 directly. We firstly demonstrated that GRh2 played a ... NKG2D-MICA signaling axis via directly binding to ERp5, and may be further optimized to a therapeutic ...
Tópico(s): Pharmacological Effects of Natural Compounds
2023 - Elsevier BV | Phytomedicine
Angelina J. Lay, Alexander Dupuy, Lejla Hagimola, Jessica Tieng, Mark Larance, Yunwei Zhang, Jean Yang, Yvonne Kong, Joyce Chiu, E. E. D. Gray, Zihao Qin, Diana Schmidt, Jessica MacLean, Benjamin R. Hofma, Marc Ellis, Maggie L. Kalev‐Zylinska, Yair Argon, Shaun P. Jackson, Philip J. Hogg, Freda Passam,
... endoplasmic reticulum protein 57 (ERp57), ERp72, ERp46, and ERp5, are required for in vivo thrombus formation in ... Here, we aim to characterize the role of ERp5 (gene Pdia6) using platelet conditional knockout mice, platelet ... fl mice developed mild macrothrombocytopenia. Platelets deficient in ERp5 showed marked dysregulation of their ER, indicated by ... 78 kilodalton glucose-regulated protein (GRP78), and calreticulin. ERp5-deficient platelets showed an enhanced ER stress response ... factor 2A and inositol-requiring enzyme 1 (IRE1). ERp5 deficiency was associated with increased secretion of PDIs, ...
Tópico(s): Pancreatic function and diabetes
2022 - Elsevier BV | Blood Advances
Produção Nacional
Revisado por pares
Jean-Paul Smets-Solanes, Rogério Atem de Carvalho,
Our architecture, ERP5, offers several advantages for small and medium-size companies. All ERP5 tools are open source, so are free and ... a business can change to suit its processes. ERP5 incorporates, from scratch, advanced concepts such as object- ... a method to model and implement business processes. ERP5 is also a Web site where researchers can ...
Tópico(s): Business Process Modeling and Analysis
2003 - IEEE Computer Society | IT Professional
Kiranmai Gumireddy, Fangxian Sun, Andres J. Klein–Szanto, Jonathan M. Gibbins, Phyllis A. Gimotty, Aleister J. Saunders, Peter G. Schultz, Qihong Huang,
... the lung. The genes encoding the disulfide isomerase ERp5 and β-catenin were found to promote breast ... cancer cell signaling and tumor metastasis. Overexpression of ERp5 promotes both in vitro migration and invasion and ... ErbB2 and PI3K reverses the phenotypes induced by ERp5. Finally, ERp5 was shown to be up-regulated in human ...
Tópico(s): Wnt/β-catenin signaling in development and cancer
2007 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Ryogo Hirata, Coh-ichi Nihei, Akihiko Nakano,
... as reported previously. A second mutation in either Erp5 or Erp6 aggravated the erp1Δ phenotype, and the ... and form an additional αβγδ complex with Erp3, Erp5, and Emp24. This complex was mainly localized to ... as reported previously. A second mutation in either Erp5 or Erp6 aggravated the erp1Δ phenotype, and the ... and form an additional αβγδ complex with Erp3, Erp5, and Emp24. This complex was mainly localized to ...
Tópico(s): Peptidase Inhibition and Analysis
2013 - Elsevier BV | Journal of Biological Chemistry
Produção Nacional
Revisado por pares
Renato Simões Gaspar, Samira Abdalla da Silva, Jennifer Stapleton, João Lucas de Lima Fontelles, Hiran Reis Sousa, Vinicyus Teles Chagas, Shuruq E. Alsufyani, Andrés Trostchansky, Jonathan M. Gibbins, Antonio Marcus de Andrade Paes,
... despite inhibiting only protein disulphide isomerase (PDI) and ERp5 reductase activities (IC50~3.5 μM). Finally, molecular ... myricetin formed non-covalent bonds with PDI and ERp5. Conclusions: PESc and its most abundant flavonoid myricetin ... function. Additionally, myricetin is a novel inhibitor of ERp5 and PDI, unveiling a new therapeutic perspective for ...
Tópico(s): Calcium signaling and nucleotide metabolism
2020 - Frontiers Media | Frontiers in Pharmacology
Bruce Furie, Robert Flaumenhaft,
Protein disulfide isomerase (PDI), ERp5, and ERp57, among perhaps other thiol isomerases, are important for the initiation of thrombus formation. Using the laser injury thrombosis ... models in mice have demonstrated the importance of ERp5 and ERp57 in the initiation of thrombus formation. ... in this process and interact directly with PDI, ERp5, and ERp57. The mechanism by which thiol isomerases ...
Tópico(s): Toxin Mechanisms and Immunotoxins
2014 - Lippincott Williams & Wilkins | Circulation Research
Congrong Wang, David W. Essex,
... this reagent was recently found to inhibit ERp57, ERp5 and thioredoxin, and to inhibit both active sites ... family are also potential targets for antithrombotic therapy. ERp5 and ERp57, the closest homologue of PDI in ... M. A role for the thiol isomerase protein ERP5 in platelet function.Blood. 2005; 105: 1500-7Crossref ... Furie B. Both platelet‐ and endothelial cell‐derived ERp5 support thrombus formation in a laser‐induced mouse ...
Tópico(s): Advanced Fluorescence Microscopy Techniques
2017 - Elsevier BV | Journal of Thrombosis and Haemostasis
Maria Raffaella Zocchi, Silvia Catellani, Paolo Canevali, Sara Tavella, Anna Garuti, Barbara Villaggio, Annalisa Zunino, Marco Gobbi, Giulio Fraternali Orcioni, Annalisa Kunkl, Jean-Louis Ravetti, Silvia Boero, Alessandra Musso, Alessandro Poggi,
... of transcription and expression of the disulfide-isomerase ERp5 and of the disintegrin-metalloproteinase ADAM10, able to ...
Tópico(s): CAR-T cell therapy research
2011 - Elsevier BV | Blood
Peter A. Jordan, Jonathan M. Gibbins,
... thiol isomerases, which include protein disulfide isomerase (PDI), ERp5, ERp57, and ERp72. While these enzymes were reported ... of function blocking antibodies to either PDI or ERp5 causes inhibition of these functional responses. This review ...
Tópico(s): Erythrocyte Function and Pathophysiology
2006 - Mary Ann Liebert, Inc. | Antioxidants and Redox Signaling
Teresa M. Brophy, Barry S. Coller, Jasimuddin Ahamed,
... Willebrand factor, multimerin-1, protein disulfide isomerase (PDI), ERp5, ERp57, and ERp72 eluted specifically from the column. ... Willebrand factor, multimerin-1, protein disulfide isomerase (PDI), ERp5, ERp57, and ERp72 eluted specifically from the column. ...
Tópico(s): Blood properties and coagulation
2013 - Elsevier BV | Journal of Biological Chemistry
Christine N. Galinski, Jeffrey I. Zwicker, Daniel R. Kennedy,
... substrate. The inhibition of PDI related thiol isomerases ERp5 and ERp57 was also assessed. Results We observed ... wine also inhibited the PDI related thiol isomerases ERp5 and ERp57, albeit to a lesser degree than ...
Tópico(s): Biochemical effects in animals
2015 - Elsevier BV | Thrombosis Research
Freda Passam, Joyce Chiu, Lining Arnold Ju, Aster E. Pijning, Zeenat Jahan, Ronit Mor-Cohen, Adva Yeheskel, Katra Kolšek, Lena Thärichen, Camilo Aponte‐Santamaría, Frauke Gräter, Philip J. Hogg,
... receptor. Our studies of the secreted platelet oxidoreductase, ERp5, have revealed that it mediates release of fibrinogen ... βI-domain Cys177-Cys184 disulfide bond cleavable by ERp5. Fluid shear and force spectroscopy assays indicate that ...
Tópico(s): Venomous Animal Envenomation and Studies
2018 - eLife Sciences Publications Ltd | eLife
Yoshihiro Morimoto, Nami Yamashita, Tatsuaki Daimon, Haruka Hirose, Shizuka Yamano, Naoki Haratake, Satoshi Ishikawa, Atrayee Bhattacharya, Atsushi Fushimi, Rehan Ahmad, Hidekazu Takahashi, Olga Dashevsky, Constantine S. Mitsiades, Donald Küfe,
... was determined by ELISA. MUC1-C interactions with ERp5 and RAB27A were assessed by coimmunoprecipitation and direct ... MICA/B cleavage. Mechanistically, MUC1-C regulates the ERp5 thiol oxidoreductase that is necessary for MICA/B ...
Tópico(s): Immunotherapy and Immune Responses
2023 - BMJ | Journal for ImmunoTherapy of Cancer
Alexander Dupuy, Freda Passam,
Endoplasmic reticulum protein 5 (ERp5) is a member of the thiol isomerase family of enzymes, whose prototype member is protein disulphide isomerase (PDI). Thiol isomerases ... by chaperone activity. The disulphide redox activity of ERp5 can be measured with functional assays involving artificial ... Herein we describe step-by-step assays of ERp5 reductase, isomerization, and de-nitrosylation activity. Disulphide reductase ... These assays can measure the activity of purified ERp5 protein but can also be applied for the ...
Tópico(s): Hemoglobin structure and function
2019 - Springer Science+Business Media | Methods in molecular biology
... MICA), which is a ligand for NKG2D, and ERp5, a protein disulfide isomerase involved in MICA shedding. ...
Tópico(s): Immunotherapy and Immune Responses
2009 - Wiley | Annals of the New York Academy of Sciences
Xuanjun Wang, Ashley D. Lundgren, Pragya Singh, David R. Goodlett, Stephen R. Plymate, Jennifer D. Wu,
... is critical for the interaction of MIC with ERp5 to enable shedding. Mutations in this motif prevented ...
Tópico(s): T-cell and B-cell Immunology
2009 - Elsevier BV | Biochemical and Biophysical Research Communications
Lisa‐Marie Holbrook, Nicholas A. Watkins, A. D. Simmonds, Chris I. Jones, Willem H. Ouwehand, Jonathan M. Gibbins,
... disulphide isomerase (PDI) and endoplasmic reticulum protein 5 (ERp5) are released by resting and activated platelets. These ...
Tópico(s): Blood properties and coagulation
2009 - Wiley | British Journal of Haematology
Catia Fonseca, Robert J. Soiffer, Vincent T. Ho, Matthew W. Vanneman, Masahisa Jinushi, Jerome Ritz, Donna Neuberg, Richard M. Stone, D. J. Deangelo, Glenn Dranoff,
... setting of nonmyeloablative allogeneic bone marrow transplantation. Moreover, ERp5, a closely related disulfide isomerase involved in major ...
Tópico(s): Immune Cell Function and Interaction
2008 - Elsevier BV | Blood
Rogério Atem de Carvalho, Rafael Manhães Monnerat,
... the free and open source ERP systems, only ERP5 offers a comprehensive set of tools tailored to ...
Tópico(s): Software System Performance and Reliability
2008 - IEEE Computer Society | IT Professional
Masahisa Jinushi, Matthew W. Vanneman, Nikhil C. Munshi, Yu-Tzu Tai, Rao Prabhala, Jerome Ritz, Donna Neuberg, Kenneth C. Anderson, Daniel R. Carrasco, Glenn Dranoff,
... intermediate MICA levels and a high expression of ERp5, a protein disulfide isomerase linked to MICA shedding ( ...
Tópico(s): Multiple Myeloma Research and Treatments
2008 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Adam Glen, Caroline A. Evans, Chee Sian Gan, Simon S. Cross, Freddie C. Hamdy, Jonathan M. Gibbins, Jenny M. Lippitt, Colby L. Eaton, Josselin Noirel, Phillip C. Wright, Ishtiaq Rehman,
... qRT‐PCR). Immunostaining of prostate tissue microarrays for ERp5, one of the candidates identified, showed a significant ...
Tópico(s): Mass Spectrometry Techniques and Applications
2010 - Wiley | The Prostate
... caldesmon-1 and vimentin), endoplasmic reticulum proteins (ERp57, ERp5, and HSP47), and nuclear proteins (lamin C, heterogeneous ...
Tópico(s): Virus-based gene therapy research
2010 - Elsevier BV | Journal of Biological Chemistry
Lisa‐Marie Holbrook, Parvathy Sasikumar, R. G. STANLEY, A. D. Simmonds, Andrew B. Bicknell, Jonathan M. Gibbins,
... important roles for the thiol isomerases PDI and ERp5 in the regulation of normal platelet function.Recently, ...
Tópico(s): Erythrocyte Function and Pathophysiology
2011 - Elsevier BV | Journal of Thrombosis and Haemostasis
Tópico(s): Information Technology Governance and Strategy
2012 - Springer Science+Business Media | HMD Praxis der Wirtschaftsinformatik
Leticía Huergo-Zapico, Ana Pilar González-Rodríguez, Juan Contesti, Esther González, Alejandro López‐Soto, Azahara Fernández-Guizán, Andrea Acebes‐Huerta, Juan R. de los Toyos, Carlos López‐Larrea, Veronika Groh, Thomas A. Spies, Segundo González,
Tópico(s): Calcium signaling and nucleotide metabolism
2012 - Springer Science+Business Media | Cancer Immunology Immunotherapy
Gloria Esteso, Elisa Luzón, E. Sarmiento, Ruth Gómez-Caro, Alexander Steinle, Gillian Murphy, Javier Carbone, Mar Valés‐Gómez, Hugh T. Reyburn,
... be modulated by expression of the thiol isomerase ERp5. In this article, we describe that an increased ...
Tópico(s): T-cell and B-cell Immunology
2014 - American Association of Immunologists | The Journal of Immunology
... over 100-fold selectivity for PDI compared to ERp5, ERp57 and thioredoxin. Implications: The compounds identified in ...
Tópico(s): Cultural Competency in Health Care
2013 - Elsevier BV | American Journal of Pharmaceutical Education