
Marcos Andrade de Oliva, William Marcatti Amarú Maximiano, Larissa Moreira Spinola de Castro Raucci, Paulo Eliandro da Silva, Roger Rodrigo Fernandes, Pietro Ciancaglini, Márcio Mateus Beloti, Antonio Nanci, Adalberto Luiz Rosa, Paulo Tambasco de Oliveira,
Despite wide clinical application, the efficacy of platelet-rich plasma (PRP) for repairing bone defects and enhancing osseointegration of metal implants is still subject of debate. This study aimed to evaluate the effects of a well-defined PRP-like mixture containing platelet-derived growth factor-BB, transforming growth factor (TGF)-β1, TGF-β2, albumin, fibronectin, and thrombospondin [growth factors (GFs) + proteins] on the development of the osteogenic phenotype on titanium (Ti) in vitro. Human alveolar ...
Tópico(s): Bone Tissue Engineering Materials
2008 - SAGE Publishing | Journal of Histochemistry & Cytochemistry

Neyva Maria Lopes Romeiro, Rigoberto Gregório Sanabria Castro, Eliandro Rodrigues Cirilo, Paulo Laerte Natti,
The calibration of a model refers to the process by which one can estimate some parameters by comparisons with observed data. Due to the dynamical nature of the environment, variations between predicted and observed values occur. Thus, the environmental parameters may vary due to random temperature changes, time of discharge flow, time of the day, and other conditions. Such variations can be minimized by identifying and optimizing some parameters of the transport model, like the values of diffusion ...
Tópico(s): Dam Engineering and Safety
2011 - Elsevier BV | Ecological Modelling
Eliandro José Gutierres Figueira, Everton Cazzo, Paula Tuma, Carlos Rodrigues da Silva Filho, Lucieni de Oliveira Conterno,
dos princípios morais clássicos 2 .
Tópico(s): Legal Education and Practice Innovations
2004 - Brazilian Medical Association | Revista da Associação Médica Brasileira

Eliane Saori Otaguiri, Ana Elisa Belotto Morguette, Eliandro Reis Tavares, Pollyanna Myrella Capela dos Santos, Alexandre Tadachi Morey, J. Cardoso, Márcia Regina Eches Perugini, Lucy Megumi Yamauchi, Sueli Fumie Yamada‐Ogatta,
Streptococcus agalactiae or Group B Streptococci (GBS) have the ability to access various host sites, which reflects its adaptability to different environments during the course of infection. This adaptation is due to the expression of virulence factors that are involved with survival, invasion and bacterial persistence in the host. This study aimed to characterize GBS isolates from women of reproductive age seen at University Hospital of Londrina, according to capsular typing, genetic relatedness, ...
Tópico(s): Bacterial Identification and Susceptibility Testing
2013 - BioMed Central | BMC Microbiology
Eliandro Santos, Carina Marinho,
Resultados: As principais causas encontradas foram a sépsis, o choque sético e as doenças respiratórias e cardiovasculares.Obteve-se como principais intervenções de enfermagem a prevenção de choque, a regulação hemodinâmica e o controle ácido-básico e da infeção.Conclusão: O presente estudo fornece as ações de enfermagem de acordo com a Classificação das Intervenções de Enfermagem (Nursing Intervention Classification -NIC), para que o enfermeiro possa identificar precocemente e de forma sistematizada ...
Tópico(s): Public Health in Brazil
2013 - Health Sciences Research Unit: Nursing (UICISA: E) | Revista de Enfermagem Referência

Thomás da Silva Rocha, Eliandro Silva Nascimento, Adilson C. Silva, Henrique S. Oliveira, Eric M. Garcia, Luiz Carlos Alves de Oliveira, D. S. Monteiro, Mariandry Rodríguez, Márcio C. Pereira,
Ni(OH)2 loaded on Ni-doped δ-FeOOH photocatalysts were prepared by a simple and low-cost one-step precipitation method. The effect of Ni(OH)2 nanoparticles and Ni2+ doping on the photocatalytic hydrogen production rates by δ-FeOOH in aqueous suspension was investigated. The results showed that the photocatalytic H2-production activity of δ-FeOOH was significantly enhanced by doping with Ni2+ ions and by loading Ni(OH)2 on its surface. The maximum H2-production was obtained for the sample with 20 ...
Tópico(s): Copper-based nanomaterials and applications
2013 - Royal Society of Chemistry | RSC Advances

Alexandro Cézar Faleiro, Tomás Pellizzaro Pereira, Eliandro Espindula, Fábio Cristiano Angonesi Brod, Ana Carolina Maisonnave Arisi,
Tópico(s): Plant Pathogenic Bacteria Studies
2013 - Springer Science+Business Media | Symbiosis

Fernanda M. Souza, Kalina M. Brauko, Eliandro R. Gilbert, César C. Martins, Paulo da Cunha Lana, Mauricio Camargo Guerrero,
To better understand variation in sewage-impacted benthic macrofauna from subtropical tidal flats over time and space, we applied a five-factor linear model at a hierarchy of spatial (Condition - Contaminated or Non-Contaminated, Tidal Flat and Plot) and temporal scales (Season and Fortnight). The Contaminated site showed high levels of coprostanol and the presence of Paranais cf frici as markers or indicators of organic enrichment. Differences between Seasons were more pronounced for the faunal variation ...
Tópico(s): Marine and coastal plant biology
2016 - Elsevier BV | Marine Environmental Research

Jéssica Hellen Souza da Silva, Naomi Kato Simas, Celuta S. Alviano, Daniela Sales Alviano, José Aires Ventura, Eliandro J. de Lima, Sérgio Henrique Seabra, Ricardo Machado Kuster,
Ethanol extracts obtained from Schinus terebinthifolius Raddi fruits and leaves were active against Escherichia coli with MIC of 78 μg mL-1 for both extracts. Phytochemical analyses revealed a major presence of phenolic acids, tannins, fatty acids and acid triterpenes in the leaves and phenolic acids, fatty acids, acid triterpenes and biflavonoids in the fruits. Major compounds isolated from the plant, such as the acid triterpene schinol, the phenolic acid derivative ethyl gallate and the biflavonoids ...
Tópico(s): Medicinal Plants and Neuroprotection
2017 - Taylor & Francis | Natural Product Research

Eliane Saori Otaguiri, Ana Elisa Belotto Morguette, Alexandre Tadachi Morey, Eliandro Reis Tavares, Gilselena Kerbauy, Rosângela Stadnick Lauth de Almeida Torres, Mauricio Chaves Júnior, Maria Cristina Bronharo Tognim, Viviane Monteiro Góes, Marco Aurélio Krieger, Márcia Regina Eches Perugini, Lucy Megumi Yamauchi, Sueli Fumie Yamada‐Ogatta,
Streptococcus agalactiae or Group B Streptococcus (GBS) remains the leading cause of infections in newborns worldwilde. Prenatal GBS screening of pregnant women for vaginal-rectal colonization is recommended in many countries to manage appropriate intrapartum antimicrobial prophylaxis for those identified as carriers. In this study, a novel melting-curve based multiplex real-time PCR assay for the simultaneous detection of GBS and macrolide and lincosamide resistance markers was developed. The usefulness ...
Tópico(s): Infective Endocarditis Diagnosis and Management
2018 - BioMed Central | BMC Pregnancy and Childbirth

Felipe Crepaldi Duarte, Tiago Danelli, Eliandro Reis Tavares, Ana Elisa Belotto Morguette, Gilselena Kerbauy, Cíntia Magalhães Carvalho Grion, Lucy Megumi Yamauchi, Márcia Regina Eches Perugini, Sueli Fumie Yamada‐Ogatta,
mecA-positive oxacillin phenotypically susceptible Staphylococcus aureus (OS-MRSA) is increasingly reported worldwide. This bacterium poses a therapeutic threat, as it can be misidentified as an oxacillin-susceptible organism by phenotypic methods that are routinely used in the majority of clinical microbiology laboratories. Herein, we report the first case of fatal sepsis in a 43-year-old female patient caused by an OS-MRSA SCCmec type IVa/ST1/CC1 in a tertiary hospital in southern Brazil, which ...
Tópico(s): Antibiotic Resistance in Bacteria
2018 - Elsevier BV | Journal of Infection and Chemotherapy

César Armando Contreras Lancheros, Milena T. Pelegrino, Danielle Kian, Eliandro Reis Tavares, Priscila M. Hiraiwa, Samuel Goldenberg, Celso Vataru Nakamura, Lucy Megumi Yamauchi, Phileno Pinge‐Filho, Amedea B. Seabra, Sueli Fumie Yamada‐Ogatta,
Chagas' disease, caused by Trypanosoma cruzi, was described for the first time over a hundred years ago. Nonetheless, clinically available drugs still lack effective and selective properties. Nitric oxide (NO) produced by activated macrophages controls the progression of disease by killing the parasite.Here, chitosan nanoparticles (CS NPs) were synthesized and mercaptosuccinic acid (MSA), the NO donor precursor, was encapsulated into CS NPs, forming MSA-CS NPs, which had hydrodynamic size of 101.0± ...
Tópico(s): Studies on Chitinases and Chitosanases
2018 - Bentham Science Publishers | Current Pharmaceutical Design

Paulo Eliandro da Silva Júnior, Hawraz Ibrahim M. Amin, Alexander M. Nauth, Flávio da Silva Emery, Stefano Protti, Till Opatz,
Abstract Arylazo sulfones have been established as photoactive precursors in visible‐light photoreactions without any additional catalysts. The arylation of various (hetero) aromatic substrates and isonitriles was carried in a simple solar microcapillary reactor in three different geographical locations (Germany, Italy and Brazil). High conversion of the starting substrates as well as short reaction times were observed.
Tópico(s): Radical Photochemical Reactions
2018 - Wiley | ChemPhotoChem

Ana Elisa Belotto Morguette, Briani Gisele Bigotto, Renata de Lima Varella, Gabriella Maria Andriani, Laís Fernanda de Almeida Spoladori, Patrícia Moraes Lopes Pereira, Fábio Goulart de Andrade, César Armando Contreras Lancheros, Celso Vataru Nakamura, Nilton Syogo Arakawa, Marcos Luciano Bruschi, José Carlos Tomaz, Audrey Alesandra Stinghen Garcia Lonni, Gilselena Kerbauy, Eliandro Reis Tavares, Lucy Megumi Yamauchi, Sueli Fumie Yamada‐Ogatta,
Streptococcus agalactiae or Group B Streptococcus (GBS) remains a leading cause of neonatal infections worldwide; and the maternal vaginal-rectal colonization increases the risk of vertical transmission of GBS to neonates and development of infections. This study reports the in vitro antibacterial effect of the oleoresin from Copaifera officinalis Jacq. L. in natura (copaiba oil) and loaded into carbomer-hydrogel against planktonic and sessile cells of GBS. First, the naturally extracted copaiba oil ...
Tópico(s): Natural product bioactivities and synthesis
2019 - Frontiers Media | Frontiers in Microbiology

Eliandro Reis Tavares, Bárbara Gionco, Ana Elisa Belotto Morguette, Gabriella Maria Andriani, Alexandre Tadachi Morey, Anderson Oliveira do Carmo, Ulisses de Pádua Pereira, Galdino Andrade, Admilton Gonçalves de Oliveira, Phileno Pinge‐Filho, Celso Vataru Nakamura, Lucy Megumi Yamauchi, Sueli Fumie Yamada‐Ogatta,
In this study, we characterized Cryptococcus gattii biofilm formation in vitro. There was an increase in the density of metabolically active sessile cells up to 72 h of biofilm formation on polystyrene and glass surfaces. Scanning electron microscopy and confocal laser scanning microscopy analysis revealed that in the early stage of biofilm formation, yeast cells adhered to the abiotic surface as a monolayer. After 12 h, extracellular fibrils were observed projecting from C. gattii cells, connecting ...
Tópico(s): Antimicrobial Peptides and Activities
2019 - Nature Portfolio | Scientific Reports

Milena Menegazzo Miranda-Sapla, Fernanda Tomiotto‐Pellissier, João Paulo Assolini, Amanda Cristina Machado Carloto, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Eliandro Reis Tavares, Jean Henrique da Silva Rodrigues, Andréa Name Colado Simão, Lucy Megumi Yamauchi, Celso Vataru Nakamura, Waldiceu A. Verri, Idessânia Nazareth Costa, Ivete Conchon‐Costa, Wander Rogério Pavanelli,
Leishmania parasites infect macrophages causing a wide spectrum of human diseases encompassing from cutaneous to visceral forms. The drugs currently used in leishmaniasis treatment are highly toxic and associated with acquired resistance. Seeking novel therapeutic targets, we conducted a comprehensive in vitro study to investigate the action of trans-chalcone (TC) against Leishmania amazonensis promastigote and amastigote forms. TC is a common precursor of flavonoids, however, no extensive research ...
Tópico(s): Trypanosoma species research and implications
2019 - Elsevier BV | European Journal of Pharmacology

Tiago Danelli, Felipe Crepaldi Duarte, Thilara Alessandra de Oliveira, Raquel Soares da Silva, Daniela Frizon Alfieri, Guilherme Bartolomeu Gonçalves, Caio Ferreira de Oliveira, Eliandro Reis Tavares, Lucy Megumi Yamauchi, Márcia Regina Eches Perugini, Sueli Fumie Yamada‐Ogatta,
Background. Staphylococcus aureus can asymptomatically colonize the human anterior nares and skin, and nasal colonization by this bacterium represents a potential risk for development of invasive infections. The aim of this study was to determine the prevalence of S. aureus nasal carriage among healthcare workers and students attending a university hospital and to characterize the isolates phenotypically and molecularly. Methods. A cross-sectional study was performed with 324 volunteers. Cultures ...
Tópico(s): Streptococcal Infections and Treatments
2020 - Hindawi Publishing Corporation | Interdisciplinary Perspectives on Infectious Diseases

Alexandre Fernandes Santos, Pedro Dinis Gaspar, Aseel Hamandosh, Eliandro Barbosa de Aguiar, Antonio Carlos Guerra Filho, Heraldo José Lopes de Souza,
The spread of Coronavirus is causing in the society all around the world a considerable degree of fear, worry and concern and particularly among healthcare workers that are at increased risk for infection. This paper gathers the strategy/guidelines to reduce the contamination in Intensive care unit (ICU) and in all the hospital environment. The ASHRAE and REHVA guidelines applied the UV-C Lamps, Pressure control filtration, Restroom actions and Humidity control to reduce the coronavirus disease (Covid- ...
Tópico(s): Climate Change and Health Impacts
2020 - Instituto de Tecnologia do Paraná (Tecpar) | Brazilian Archives of Biology and Technology

Erika Kushikawa Saeki, Heloísa Moreira Martins, Larissa Ciappina de Camargo, Laís Anversa, Eliandro Reis Tavares, Sueli Fumie Yamada‐Ogatta, Lucy Megumi Yamauchi, Renata Katsuko Takayama Kobayashi, Gerson Nakazato,
The increase in multidrug-resistant microorganisms represents a global threat requiring the development novel strategies to fight bacterial infection. This study aimed to assess the effect of silver nanoparticles (bio-AgNPs) on bacterial growth, biofilm formation, production of virulence factors, and expression of genes related to the quorum-sensing (QS) system of P. aeruginosa PAO1 and PA14. Biofilm formation and virulence assays were performed with bio-AgNPs. RT-qPCR was carried out to determine ...
Tópico(s): Antimicrobial Peptides and Activities
2022 - Multidisciplinary Digital Publishing Institute | Microorganisms

Eliandro Reis Tavares, Caroline Souza Azevedo, Luciano Aparecido Panagio, Marsileni Pelisson, Phileno Pinge‐Filho, Emerson José Venâncio, Tânia Fraga Barros, Sueli Fumie Yamada‐Ogatta, Lucy Megumi Yamauchi,
In this work, two accurate and sensitive real-time polymerase chain reaction (PCR) assays to differentiate pathogenic Cryptococcus gattii sensu lato ( s.l. ) and C. neoformans sensu lato ( s.l. ) targeting the intergenic spacer 1 (IGS1) region from rDNA locus were developed. Specific primers were designed based on their IGS1 sequence analyses and the optimal real-time PCR assays showed that the dissociation curves generated two different melting peaks, at 82.8 and 84.2ºC for C. gattii s.l. and C. neoformans ...
Tópico(s): Antifungal resistance and susceptibility
2015 - Oxford University Press | Medical Mycology

Bárbara Gionco, Eliandro Reis Tavares, Admilton Gonçalves de Oliveira, Sueli Fumie Yamada‐Ogatta, Anderson Oliveira do Carmo, Ulisses de Pádua Pereira, Roberta Torres Chideroli, Ane Stéfano Simionato, Miguel Octavio Pérez Navarro, Andréas Lazaros Chryssafidis, Galdino Andrade,
The bacterial resistance for antibiotics is one of the most important problems in public health and only a small number of new products are in development. Antagonistic microorganisms from soil are a promising source of new candidate molecules. Products of secondary metabolism confer adaptive advantages for their producer, in the competition for nutrients in the microbial community. The biosynthesis process of compounds with antibiotic activity is the key to optimize their production and the transcriptomic ...
Tópico(s): Antibiotic Resistance in Bacteria
2017 - Frontiers Media | Frontiers in Chemistry

Rito Santo Pereira, Aparecida Donizette Malvezi, Maria Isabel Lovo‐Martins, Bruno Fernando Cruz Lucchetti, Jussevania Pereira Santos, Eliandro Reis Tavares, Waldiceu A. Verri, Eduardo José de Almeida Araújo, Lucy Megumi Yamauchi, Sueli Fumie Yamada‐Ogatta, Marli Cardoso Martins‐Pinge, Phileno Pinge‐Filho,
Chagas disease, caused by the protozoan Trypanosoma cruzi, is one of the main causes of death due to cardiomyopathy and heart failure in Latin American countries. The treatment of Chagas disease is directed at eliminating the parasite, decreasing the probability of cardiomyopathy and disrupting the disease transmission cycle. Benznidazole (BZ) and nifurtimox (Nfx) are recognized as effective drugs for the treatment of Chagas disease by the World Health Organization, but both have high toxicity and ...
Tópico(s): Research on Leishmaniasis Studies
2020 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy

Suellen Ribeiro Pardo, Paulo Laerte Natti, Neyva Maria Lopes Romeiro, Eliandro Rodrigues Cirilo,
This work is a contribution to a better understanding of the effect that domestic sewage discharges may cause in a water body, specifically at Igapó I Lake, in Londrina, Paraná State, Brazil. The simulation of the dynamics of pollutant concentrations throughout the water body was conducted by means of structured discretization of the geometry of Igapó I Lake, together with the finite differences and the finite elements methods. Firstly, the hydrodynamic flow (without the pollutants), modeled by Navier- ...
Tópico(s): Membrane Separation Technologies
2012 - Editora da Universidade Estadual de Maringá (Eduem) | Acta Scientiarum. Technology/Acta scientiarum. Technology

Júlia Baptista, Paula Pinheiro Sanches, Gustavo Manoel Teixeira, Alexandre Tadachi Morey, Eliandro Reis Tavares, Sueli Fumie Yamada‐Ogatta, Sérgio Paulo Dejato Rocha, Mariangela Hungría, Renan Augusto Ribeiro, María Isabel Balbi-Peña, Roberta Torres Chideroli, Ulisses de Pádua Pereira, Admilton Gonçalves de Oliveira,
Bacillus velezensis strain LABIM40 holds high potential for biological control of plant pathogens. Its complete genome contains one chromosome of 3,972,310 bp with 3,777 DNA coding sequences and displays 33 gene clusters potentially involved in the suppression of fungal pathogens.
Tópico(s): Genomics and Phylogenetic Studies
2018 - American Society for Microbiology | Genome Announcements

Paulo Eliandro da Silva Júnior, Natália Maria de Araujo, Flávio da Silva Emery,
α‐xiloidone is an interesting chromene that are structurally related to lapachones; in this work, we selectively synthesized α‐xiloidone from the reaction of lawsone 1 with 3,3‐chloro‐3‐methylbut‐1‐yne under copper/I 2 catalysis via propargyl Claisen rearrangement.
Tópico(s): Synthesis and Reactions of Organic Compounds
2014 - Wiley | Journal of Heterocyclic Chemistry

Amanda de Fáveri Pitz, Andrea Cristine Koishi, Eliandro Reis Tavares, Fábio Goulart de Andrade, Eduardo Alexandre Loth, Rinaldo Ferreira Gandra, Emerson José Venâncio,
Introduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. Methods We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and compared it with other PCR methods. Results The OTsn-PCR assay was positive for all clinical samples, and the detection limit was better or equivalent to the other nested or semi-nested PCR methods for P. brasiliensis detection. Conclusions The OTsn- ...
Tópico(s): Cytomegalovirus and herpesvirus research
2013 - Brazilian Society of Tropical Medicine | Revista da Sociedade Brasileira de Medicina Tropical

Eliandro Espindula, Edilena Reis Sperb, Evelise Bach, Luciane Maria Pereira Passaglia,
In Dual RNA-Seq experiments the simultaneous extraction of RNA and analysis of gene expression data from both interacting organisms could be a challenge. One alternative is separating the reads during in silico data analysis. There are two main mapping methods used: sequential and combined. Here we present a combined approach in which the libraries were aligned to a concatenated genome to sort the reads before mapping them to the respective annotated genomes. A comparison of this method with the ...
Tópico(s): Plant Disease Resistance and Genetics
2019 - Brazilian Society of Genetics | Genetics and Molecular Biology

Taylon Felipe Silva, Virgínia Márcia Concato, Fernanda Tomiotto‐Pellissier, Manoela Daiele Gonçalves, Bruna Taciane da Silva Bortoleti, Eliandro Reis Tavares, Lucy Megumi Yamauchi, Cíntia Magalhães Carvalho Grion, Andréa Name Colado Simão, Milena Menegazzo Miranda-Sapla, Idessânia Nazareth Costa, Wander Rogério Pavanelli, Ivete Conchon‐Costa,
Abstract CMV reactivation has been widely associated with bacterial sepsis and occurs in approximately 30% of these individuals, is associated with a longer ICU stay, prolongation of the need for mechanical ventilation, and over 80% increase in the mortality rate, being directly associated with severe organ dysfunction and hemodynamic imbalance. Thus, the aim of this study was to evaluate the role of CMV reactivation in sepsis progression. The overall occurrence of cytomegalovirus reactivation in ...
Tópico(s): Immune Response and Inflammation
2019 - Nature Portfolio | Scientific Reports

Fernanda M. Souza, Eliandro R. Gilbert, Mauricio Camargo Guerrero, Wagner W. Pieper,
Modeling the distribution patterns of the estuarine macrobenthic community has revealed itself as a difficult task due to spatio-temporal heterogeneity. This study uses ordinary kriging and Poisson modeling to generate distribution maps of the subtidal benthic macrofauna in the Trapandé Bay (southeastern Brazil). Samples were taken in duplicate from 36 locations distributed along nine transects perpendicular to the main estuarine axis in October 2006 and March 2007. One-hundred and seventy taxa belonging ...
Tópico(s): Marine Bivalve and Aquaculture Studies
2013 - Pensoft Publishers | Zoologia (Curitiba)

Ana Elisa Belotto Morguette, Guilherme Bartolomeu-Gonçalves, Gabriella Maria Andriani, Giovana Elika Silveira Bertoncini, Isabela Madeira de Castro, Laís Fernanda de Almeida Spoladori, Ariane Mayumi Saito Bertão, Eliandro Reis Tavares, Lucy Megumi Yamauchi, Sueli Fumie Yamada‐Ogatta,
Wounds of an acute or chronic etiology affect millions of people worldwide, with increasing prevalence every year. Microbial infections are one of the main causes that impair the wound healing process, and Staphylococcus aureus, a commensal member of the skin microbiota, is one of the main causative agents of wound infections. Crucially, a high proportion of these infections are caused by methicillin-resistant Staphylococcus aureus, which, in addition to β-lactams, has acquired resistance to almost ...
Tópico(s): Phytochemicals and Medicinal Plants
2023 - Multidisciplinary Digital Publishing Institute | Plants