Alexandra Binnie, Emanuel Fernandes, Hélder Almeida-Lousada, Ramon Andrade de Mello, Pedro Castelo‐Branco,
CRISPR gene-editing technology has the potential to transform the diagnosis and treatment of infectious diseases, but most clinicians are unaware of its broad applicability. Derived from an ancient microbial defence system, these so-called “molecular scissors” enable precise gene editing with a low error rate. However, CRISPR systems can also be targeted against pathogenic DNA or RNA sequences. This potential is being combined with innovative delivery systems to develop new therapeutic approaches ...
Tópico(s): Virus-based gene therapy research
2021 - Springer Science+Business Media | Infection
Produção Nacional
Revisado por pares
Adolfo Jatobá, Klayton Natan Moraes, Emanuel Fernandes Rodrigues, Leandro Marcolino Vieira, M.O. Pereira,
ABSTRACT: The aim of this research was to evaluate the changes in intestinal tract microbiota, hematological parameters and zootechnical indexes of yellowtail lambaris (Astyanax bimaculatus) fed with different probiotic supply frequencies (Lactobacillus spp.). Lambaris (1,200) were distributed in twelve boxes, divided in three treatments, 100% supply; 25% and 0% (control) of feeds supplemented with probiotic. After 87 days, fish fed with supplemented diet, regardless of frequency (100% and 25%), showed ...
Tópico(s): Aquatic life and conservation
2018 - UNIVERSIDADE FEDERAL DE SANTA MARIA | Ciência Rural
Emanuel Ferreira‐Fernandes, João Peça,
Social status is recognized as a major determinant of social behavior and health among animals; however, the neural circuits supporting the formation and navigation of social hierarchies remain under extensive research. Available evidence suggests the prefrontal cortex is a keystone in this circuit, but upstream and downstream candidates are progressively emerging. In this review, we compare and integrate findings from rodent and primate studies to create a model of the neural and cellular networks ...
Tópico(s): Neuroendocrine regulation and behavior
2022 - Frontiers Media | Frontiers in Cellular Neuroscience
Simone S. Silva, Luísa C. Rodrigues, Emanuel M. Fernandes, Flávia C. M. Lobo, Joana M. Gomes, Rui L. Reis,
Oleogels are becoming an attractive research field, since they have recently been shown to be feasible for the food and pharmaceutical sectors and provided some insights into the biomedical area. In this work, edible oleogels were tailored through the combination of ethylcellulose (EC), a gelling agent, with virgin coconut oil (VCO), vegetable oil derived from coconut. The influence of the different EC and VCO ratios on the structural, physical, and thermal properties of the oleogels was studied. ...
Tópico(s): Edible Oils Quality and Analysis
2022 - Multidisciplinary Digital Publishing Institute | Polymers
Flávia C. M. Lobo, Albina R. Franco, Emanuel M. Fernandes, Rui L. Reis,
Pathogenic microbes are a major source of health and environmental problems, mostly due to their easy proliferation on most surfaces. Currently, new classes of antimicrobial agents are under development to prevent microbial adhesion and biofilm formation. However, they are mostly from synthetic origin and present several disadvantages. The use of natural biopolymers such as cellulose, hemicellulose, and lignin, derived from lignocellulosic materials as antimicrobial agents has a promising potential. ...
Tópico(s): biodegradable polymer synthesis and properties
2021 - Multidisciplinary Digital Publishing Institute | Molecules
Produção Nacional
Eduardo Henrique Backes, Emanuel M. Fernandes, Gabriela S. Diogo, Catarina F. Marques, Tiago H. Silva, L. Costa, Fábio Roberto Passador, Rui L. Reis, Luiz Antônio Pessan,
In this study, polylactic acid (PLA) filled with hydroxyapatite (HA) or beta-tricalcium phosphate (TCP) in 5 wt% and 10 wt% of concentration were produced employing twin-screw extrusion followed by fused filament fabrication in two different architectures, varying the orientation of fibers of adjacent layers. The extruded 3D filaments presented suitable rheological and thermal properties to manufacture of 3D scaffolds envisaging bone tissue engineering. The produced scaffolds exhibited a high level ...
Tópico(s): biodegradable polymer synthesis and properties
2021 - Elsevier BV | Materials Science and Engineering C
Produção Nacional
Revisado por pares
Rafaela B. Salum, Pedro Walfir Martins e Souza Filho, Marc Simard, Carlos Alberto Silva, Marcus Emanuel Barroncas Fernandes, Michele Ferreira Cougo, Wilson do Nascimento, Kerrylee Rogers,
Tree height is a key parameter to accurately quantify above ground biomass (AGB) of trees. Approaches that integrate airborne light detection and ranging (LiDAR) with mapped extents of forests may improve estimation of mangrove heights by providing considerably more measurements of mangrove tree heights than can be achieved using field-based measurements alone. In this study, we present a validated method for quantifying mangrove AGB that was demonstrated for a mangrove forest at Guarás Island, Brazil. ...
Tópico(s): Aeolian processes and effects
2020 - Elsevier BV | Estuarine Coastal and Shelf Science
Produção Nacional
Revisado por pares
Paulo C. C. Virgulino-Júnior, Diego Novaes Carneiro, Wilson Rocha Nascimento, Michele Ferreira Cougo, Marcus Emanuel Barroncas Fernandes,
Reliable estimates of biomass and carbon storage are essential for the understanding of the environmental drivers and processes that regulate the productivity of scrub forests. The present study estimated total (above-ground, AGB + below-ground, BGB) biomass and carbon storage of a scrub forest dominated by Avicennia germinans (L.) L. based on the existing allometric models for the AGB, while novel models were developed to estimate the BGB. Data collection followed a destructive approach by using the " ...
Tópico(s): Oil Palm Production and Sustainability
2020 - Public Library of Science | PLoS ONE
Marta R. Casanova, Catarina Oliveira, Emanuel M. Fernandes, Rui L. Reis, Tiago H. Silva, Albino Martins, Nuno M. Neves,
An engineered biofunctional system comprises endogenous BMP-2 and VEGF bound in a parallel pattern. It successfully enabled obtaining the spatial osteogenic and angiogenic differentiation of human hBM-MSCs under basal culture conditions.
Tópico(s): Tissue Engineering and Regenerative Medicine
2020 - Royal Society of Chemistry | Biomaterials Science
María Fernández-Álvarez, F. Velasco, A. Bautista, Flávia C. M. Lobo, Emanuel M. Fernandes, Rui L. Reis,
Polyamide coatings are thermoplastics with great advantages such as a good corrosion protection of the base metal and wear resistance. Their application as powder coatings is an environmentally friendly option that is currently attracting growing interest. However, during their life service, they can sometimes be exposed to conditions that they are unable to stand. In this work, a polyamide 11 (PA11) powder was reinforced with different percentages of silica nanoparticles (1-3 wt. %). Powder mixtures ...
Tópico(s): biodegradable polymer synthesis and properties
2020 - Multidisciplinary Digital Publishing Institute | Polymers
Produção Nacional
Revisado por pares
Carlos Augusto França Schettini, E. Nils, A. S. Ogston, Vando José Costa Gómes, Robin McLachlan, Marcus Emanuel Barroncas Fernandes, Charles A. Nittrouer, Eliane Cristina Truccolo, Danilo Cesar Lima Gardunho,
Abstract The Amazon Macrotidal Mangrove Coast (AMMC) is a large (~7500 km 2 ) contiguous mangrove fringe eastwards from the Amazon River mouth. It encompasses dozens of interconnected bays intercalated with mangrove peninsulas. Mud accumulates on the mangrove flats, whereas the bed of the bays and channels is generally sandy. In this study we investigated the circulation, sediment transport and deposition in a central site at one of these mangrove peninsulas. The study was undertaken during the dry period, ...
Tópico(s): Geological formations and processes
2019 - Wiley | Earth Surface Processes and Landforms
Emanuel Ferreira‐Fernandes, Bárbara Pinto-Correia, Carolina Quintino, Miguel Remondes,
Memory-guided decisions depend on complex interactions between the hippocampus (HIPP) and medial mesocortical (MMC) regions, including the anterior cingulate (CG) and retrosplenial (RSC). The functional circuitry underlying these interactions is unclear. Using anatomy, electrophysiology, and optogenetics, we show that such circuitry is characterized by a functional-anatomical gradient. While the CG receives hippocampal excitatory projections originated in CA1 stratum pyramidale, the RSC additionally ...
Tópico(s): Neural dynamics and brain function
2019 - Cell Press | Cell Reports
Ricardo S. Matias, Susan Gregory, Filipe R. Ceia, Alexandra Baeta, José Seco, Miguel S. Rocha, Emanuel M. Fernandes, Rui L. Reis, Tiago H. Silva, Eduarda Pereira, Uwe Piatkowski, Jaime A. Ramos, José C. Xavier,
Sympatry can lead to higher competition under climate change and other environmental pressures, including in South Georgia, Antarctica, where the two most common octopod species, Adelieledone polymorpha and Pareledone turqueti, occur side by side. Since cephalopods are typically elusive animals, the ecology of both species is poorly known. As beaks of cephalopods are recurrently found in top predator's stomachs, we studied the feeding ecology of both octopods through the evaluation of niche overlapping ...
Tópico(s): Marine Bivalve and Aquaculture Studies
2019 - Elsevier BV | Marine Environmental Research
Produção Nacional
Revisado por pares
Sanae Nogueira Hayashi, Pedro Walfir Martins e Souza Filho, Wilson R. Nascimento, Marcus Emanuel Barroncas Fernandes,
Mangroves play an essential ecological role in the maintenance of the coastal zone and are extremely important for the socioeconomics of coastal communities. However, mangrove ecosystems are impacted by a range of anthropogenic pressures, and the loss of this habitat can be attributed primarily to the human occupation of the coastal zone. In the present study, we analyzed the spatial patterns of land use in the mangrove of the Brazilian Amazon coast, and evaluated the anthropogenic drivers of this ...
Tópico(s): Agricultural and Environmental Management
2019 - Public Library of Science | PLoS ONE
Albina R. Franco, Emanuel M. Fernandes, Márcia T. Rodrigues, Fernando Rodrigues, Manuela E. Gomes, Isabel B. Leonor, David L. Kaplan, Rui L. Reis,
Microbial infections from post-surgery or other medical-related procedure is a serious health problem. Nowadays, the research is focused on the development of new drug-free materials with antibacterial properties to prevent or minimize the risk of infections. Spider silk is known for its unique biomechanical properties allied with biocompatibility. Recombinant DNA technology allows to bioengineering spider silk with antimicrobial peptides (AMP). Thus, our goal was to bioengineered spider silk proteins ...
Tópico(s): Biochemical and Structural Characterization
2019 - Elsevier BV | Acta Biomaterialia
Gabriela S. Diogo, Estefânia L Senra, Rogério P. Pirraco, Raphaël F. Canadas, Emanuel M. Fernandes, J. Serra, Ricardo I. Pérez‐Martín, Cármen G. Sotelo, Alexandra P. Marques, P. González, Joana Moreira‐Silva, Tiago H. Silva, Rui L. Reis,
The high prevalence of bone defects has become a worldwide problem. Despite the significant amount of research on the subject, the available therapeutic solutions lack efficiency. Autografts, the most commonly used approaches to treat bone defects, have limitations such as donor site morbidity, pain and lack of donor site. Marine resources emerge as an attractive alternative to extract bioactive compounds for further use in bone tissue-engineering approaches. On one hand they can be isolated from ...
Tópico(s): Protein Hydrolysis and Bioactive Peptides
2018 - Multidisciplinary Digital Publishing Institute | Marine Drugs
Produção Nacional
Marcus Emanuel Barroncas Fernandes, Francisco Pereira de Oliveira, Indira A. L. Eyzaguirre,
Mangroves are enormously important for the survival of traditional communities found in coastal zones around the world. This is typical of many areas in Brazil, in particular on the Amazon coast, which encompasses the largest continuous tract of mangrove forest found anywhere in the world. A multidisciplinary approach was used to describe the patterns of appropriation and uses of the resources available in this ecosystem, through the investigation of the local ecological knowledge of the traditional ...
Tópico(s): Fish biology, ecology, and behavior
2018 - Springer International Publishing | Coastal research library
Produção Nacional
Revisado por pares
Clara Grilo, Michely R. Coimbra, Rafaela Cobuci Cerqueira, Priscilla Barbosa, Rubem A.P. Dornas, Larissa Oliveira Gonçalves, Fernanda Zimmermann Teixeira, Igor Pfeifer Coelho, Brenda R. Schmidt, Diana L. K. Pacheco, Gabriela Schuck, Isadora B. Esperando, Juan Andres Anza, Júlia Beduschi, Nicole R. Oliveira, Paula Fabiana Pinheiro, Alex Bager, Helio Secco, Marcello Guerreiro, Carine Firmino Carvalho-Roel, Aline Carneiro Veloso, Ana Elizabeth Iannini Custódio, Oswaldo Marçal, Giordano Ciocheti, Julia Camara Assis, Milton Cézar Ribeiro, Beatriz S. S. Francisco, Jorge José Cherem, Tatiane Campos Trigo, Márcia Maria de Assis Jardim, Ingridi Camboim Franceschi, Caroline Espinosa, Flávia Pereira Tirelli, Vlamir José Rocha, Margareth Lumy Sekiama, Gedimar Pereira Barbosa, Helen Regina da Silva Rossi, Tainah Cruz Moreira, Marcelo Cervini, Clarissa Rosa, Lucas Gonçalves da Silva, Cláudia Márcia Marily Ferreira, Augusto César, Janaína Casella, Sérgio Lucena Mendes, Juliana Zina, Deivson Fraga de Oliveira Bastos, Ricardo A. T. Souza, Paulo Afonso Hartmann, Ângela Camila Deffaci, Jéssica Mulinari, Siane C. Luzzi, Tiago Rezzadori, Cassiane Kolcenti, Tiago Xavier Reis, Vanessa S. C. Fonseca, Camilo F. Giorgi, Raissa Prior Migliorini, Carlos Benhur Kasper, Cecília Bueno, Marcela Sobanski, Ana Paula Fernanda Guimarães Pereira, Fernanda Atanaena Gonçalves de Andrade, Marcus Emanuel Barroncas Fernandes, Luiz Liberato Costa Corrêa, Adriana Nepomuceno, Áureo Banhos, Wellington Hannibal, Rogério Fonseca, Lizit Alencar da Costa, Emília Patrícia Medici, Aline Croce, Karin Werther, Juliana Paula de Oliveira, Julia M. Ribeiro, Mariele de Santi, Aline Eyko Kawanami, Lívia Perles, Caroline do Couto, Daniela S. Figueiró, Eduardo Eizirik, Antonio A. Correia, Fábio Mathias Corrêa, Diego Queirolo, André L. Quagliatto, Bruno H. Saranholi, Pedro Manoel Galetti, Karen Giselle Rodríguez-Castro, Vívian S. Braz, Frederico Gustavo Rodrigues França, Gerson Buss, Josias A. Rezini, Marília Bruzzi Lion, Carolina Carvalho Cheida, Ana Cristyna Reis Lacerda, Carlos Henrique de Freitas, Fernando Venâncio, Cristina Harumi Adania, Augusto Florisvaldo Batisteli, Carla Grasiele Zanin Hegel, José A. Mantovani, Flávio Henrique Guimarães Rodrigues, Tathiana Bagatini, Nelson H. A. Curi, Luciano Emmert, Renato Herdina Erdmann, Raoni Ribeiro Guedes Fonseca Costa, Agustín G. Martinelli, Clarice V. F. Santos, Andreas Kindel,
Abstract Mortality from collision with vehicles is the most visible impact of road traffic on wildlife. Mortality due to roads (hereafter road‐kill) can affect the dynamic of populations of many species and can, therefore, increase the risk of local decline or extinction. This is especially true in Brazil, where plans for road network upgrading and expansion overlaps biodiversity hotspot areas, which are of high importance for global conservation. Researchers, conservationists and road planners face ...
Tópico(s): Marine animal studies overview
2018 - Wiley | Ecology
Sara F. Vieira, Albina R. Franco, Emanuel M. Fernandes, Sara Amorim, Helena Ferreira, Ricardo A. Pires, Rui L. Reis, Albino Martins, Nuno M. Neves,
Fish sarcoplasmic proteins (FSP) constitute around 25–30% of the total fish muscle protein. As the FSP are water soluble, FSP were isolated from fresh cod (Gadus morhua) by centrifugation. By SDS-PAGE, it was possible to determine the composition of FSP extracts (FSP-E). The FSP-E undergo denaturation at 44.12 ± 2.34° C, as characterized by differential scanning calorimetry thermograms (DSC). The secondary structure of FSP-E is mainly composed by α-helix structure, as determined by circular dichroism. ...
Tópico(s): biodegradable polymer synthesis and properties
2018 - Elsevier BV | Colloids and Surfaces B Biointerfaces
João F M Ribeiro, Sara M. Oliveira, J.L. Alves, A. J. Pedro, Rui L. Reis, Emanuel M. Fernandes, João F. Mano,
Three-dimensional (3D) printed poly(ε-caprolactone) (PCL) based scaffolds have being proposed for different tissue engineering applications. This study addresses the design and fabrication of 3D PCL constructs with different struts alignments at 90°, 45° and 90° with offset. The morphology and the mechanical behavior under uniaxial compressive load were assessed at different strain percentages. The combination of a new compressionCT device and micro computed tomography (micro-CT) allowed understanding ...
Tópico(s): Electrospun Nanofibers in Biomedical Applications
2017 - IOP Publishing | Biofabrication
Produção Nacional
Revisado por pares
Fernanda Atanaena Gonçalves de Andrade, Murilo Novaes Gomes, Wilson Uieda, A. L Begot, Ofir de S. Ramos, Marcus Emanuel Barroncas Fernandes,
The common hematophagous bat, Desmodus rotundus, is one of the main wild reservoirs of rabies virus in several regions in Latin America. New production practices and changed land use have provided environmental features that have been very favorable for D. rotundus bat populations, making this species the main transmitter of rabies in the cycle that involves humans and herbivores. In the Amazon region, these features include a mosaic of environmental, social, and economic components, which together ...
Tópico(s): Yersinia bacterium, plague, ectoparasites research
2016 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Lanna Jamile Corrêa da Costa, Marcus Emanuel Barroncas Fernandes,
Background The occurrence of outbreaks of human rabies transmitted by Desmodus rotundus in Brazil in 2004 and 2005 reinforced the need for further research into this zoonosis. Studies of knowledge and practices related to the disease will help to define strategies for the avoidance of new cases, through the identification of gaps that may affect the preventive practices. Methodology/Principal findings A semi-structured questionnaire was applied to 681 residents of twelve communities of northeastern ...
Tópico(s): Viral Infections and Outbreaks Research
2016 - Public Library of Science | PLoS neglected tropical diseases
Ivo M. Aroso, Emanuel M. Fernandes, Ricardo A. Pires, João F. Mano, Rui L. Reis,
The thermo-oxidative stability of polypropylene (PP) in composites containing 15 wt.% of cork and the performance of selected cork extracts as stabilizing additives for PP was evaluated by Oxidation Induction Time (OIT) and by Oxidation Onset Temperature (OOT). The results showed that cork increases the OIT of PP in the composite and it was identified that the cork extractives fraction is responsible for such behavior. Selected cork extracts with high antioxidant capacity (determined by dpph radical ...
Tópico(s): Natural Fiber Reinforced Composites
2015 - Elsevier BV | Polymer Degradation and Stability
Daniela Couto, Deolinda Santinha, Tânia Melo, Emanuel Ferreira‐Fernandes, Romeu A. Videira, Ana Margarida Ferreira Campos, Margarida Fardilha, Pedro Domíngues, M. Rosário M. Domingues,
Galactosylceramide (GalCer) and lactosylceramide (LacCer) are structural and signaling lipids, playing important roles in signal transduction and cell adhesion. They are especially abundant in the nervous system and in important components of the myelin sheath. Although neurodegenerative disorders are associated with increased oxidative stress and lipid oxidation, the connection between oxidative stress and glycosphingolipid modification has been scarcely addressed. In this study, we aimed to characterize ...
Tópico(s): Erythrocyte Function and Pathophysiology
2015 - Elsevier BV | Chemistry and Physics of Lipids
Emanuel M. Fernandes, Vítor M. Correlo, João F. Mano, Rui L. Reis,
This work addresses to the preparation of biocomposites resulting from the combination of different biodegradable aliphatic polyesters with cork (30 wt.%). The lignocellulosic biomass with closed cellular structure was compounded with poly(L-lactic acid) (PLLA), polyhydroxybutyrate-co-hydroxyvalerate (PHBV), poly-ε-caprolactone (PCL) and starch-poly-ε-caprolactone (SPCL) blend using a twin-screw extruder prior to injection moulding into tensile samples. The physico-mechanical and thermal properties of ...
Tópico(s): Electrospun Nanofibers in Biomedical Applications
2015 - Elsevier BV | Materials & Design
Produção Nacional
Revisado por pares
Michele Ferreira Cougo, Pedro Walfir Martins e Souza Filho, Arnaldo Q. Silva, Marcus Emanuel Barroncas Fernandes, João Eduardo Guarnetti dos Santos, Maria Carolina de Abreu, Wilson R. Nascimento, Marc Simard,
The aim of this study is to understand the relationship between radar backscattering (σ°, β° and γ) of a multi-polarized Radarsat-2 C-band image with the structural attributes of regenerating mangrove vegetation located at the mouth of the Amazon River. CBH (circumference at breast height), height and species data were collected to characterize vegetation structure and above-ground biomass (AGB) at 17 plots with a total of 3090 measured individuals. Significant relationships between the linear σ° in ...
Tópico(s): Soil erosion and sediment transport
2015 - Multidisciplinary Digital Publishing Institute | Remote Sensing
Emanuel M. Fernandes, Vítor M. Correlo, João F. Mano, Rui L. Reis,
Cork–polymer composites are one of the most promising fields of cork technology to create materials for a sustainable development. In this work, cork particles and powder were compounded with polypropylene (PP) by twin-screw extrusion and further processed by injection or compression moulding. The obtained PP/cork injection moulded composites were used to investigate the tensile properties before and after water absorption (WA) tests. The addition of polypropylene–graft–maleic anhydride (PP–g–MA) and ...
Tópico(s): Nanocomposite Films for Food Packaging
2014 - Elsevier BV | Composites Part B Engineering
Ana S. Mestre, Ricardo A. Pires, Ivo M. Aroso, Emanuel M. Fernandes, Moisés L. Pinto, Rui L. Reis, Marta A. Andrade, João Pires, Susana P. Silva, Ana P. Carvalho,
Industrial pre-treated cork – granules of expanded corkboard, prepared from renewable biomass – was used for the first time as precursor for the preparation of eco-friendly activated carbons by chemical (K2CO3 and KOH) and physical (steam) activation. Samples with different textural (microporosity/micro + mesoporosity) and surface chemistry (acidic/basic) were obtained. In the best compromise between porosity development/preparation yield, apparent surface areas ⩾900 m2 g−1 were attained. Selected samples ...
Tópico(s): Analytical chemistry methods development
2014 - Elsevier BV | Chemical Engineering Journal
Emanuel M. Fernandes, Ivo M. Aroso, João F. Mano, J. A. Covas, Rui L. Reis,
High density polyethylene (HDPE) and cork powder were compounded in a co-rotating twin-screw extruder to obtain cork-polymer composites (CPC) with improved properties. Benzoyl peroxide (BPO) was used as initiator agent, and suberin or lignin isolated from cork enhanced filler-matrix bonding and promoted mechanical reinforcement with environmental benefits. The novel composites were characterised in terms of dimensional stability, evolution of morphology, thermal and mechanical properties and their ...
Tópico(s): Postharvest Quality and Shelf Life Management
2014 - Elsevier BV | Composites Part B Engineering
Produção Nacional
Revisado por pares
Emanuel Tássio Fernandes, Paulo Araquém Ramos Cairo, Adalberto Brito de Novaes,
Os prolongados períodos de seca no Sudoeste da Bahia podem causar prejuízos ao cultivo de eucalipto, que é a principal espécie florestal cultivada nessa região. Com o propósito de se conhecer o desempenho de alguns clones de eucalipto, face às restrições hídricas dessa região, realizou-se um experimento em casa de vegetação, no qual foram avaliadas respostas fisiológicas de quatro clones de eucalipto à baixa disponibilidade de água no solo. Utilizou-se delineamento inteiramente ao acaso, no qual ...
Tópico(s): Plant Water Relations and Carbon Dynamics
2014 - UNIVERSIDADE FEDERAL DE SANTA MARIA | Ciência Rural