Catherine Moermans, Chloé Brion, Grégory Bock, Sophie Graff, Sara Gerday, Haleh Nekoee, Christophe Poulet, Noémie Bricmont, Monique Henket, Virginie Paulus, Françoise Guissard, Renaud Louis, Florence Schleich,
... blood eosinophil count < 300 cells/μL. Eosinophil peroxidase (EPX), IgE, IL-3, IL-4, IL-5, IL- ... addition, the sputum eotaxin-1, TSLP, IL-5, EPX, and IgE protein levels were higher at baseline ... sputum neutrophil percentage, eotaxin-1, IL-5, and EPX were potential predictors of remission.InterpretationSputum type 2 ... blood eosinophil count < 300 cells/μL. Eosinophil peroxidase (EPX), IgE, IL-3, IL-4, IL-5, IL- ... addition, the sputum eotaxin-1, TSLP, IL-5, EPX, and IgE protein levels were higher at baseline ... sputum neutrophil percentage, eotaxin-1, IL-5, and EPX were potential predictors of remission. Sputum type 2 ...
Tópico(s): Respiratory and Cough-Related Research
2023 - Elsevier BV | CHEST Journal
Shaina Hasan, Steve M. Taylor, Shipra Garg, Matthew Buras, Alfred D. Doyle, Cindy S. Bauer, Benjamin L. Wright, Shauna Schroeder,
... eosinophils. Our objective was to evaluate eosinophil peroxidase (EPX) immunohistochemistry (IHC) as a method for histologic diagnosis ... eosinophil counts were assessed by hematoxylin & eosin stains. EPX stains were assessed using a unique histopathologic scoring system. Slides were digitized and EPX+ staining area/mm 2 was quantified by image ... EG/EoD cases and 40 controls were analyzed. EPX scores and EPX/mm 2 levels were markedly elevated in EG/ ... Eosinophil density (eos/mm 2 ) correlated strongly with EPX scores and EPX/mm 2 levels in the ...
Tópico(s): Gastrointestinal disorders and treatments
2021 - SAGE Publishing | Pediatric and Developmental Pathology
Jorge Sánchez, Andrés Sánchez, Marlon Múnera, Elizabeth García, Juan‐Felipe Lopez, Margarita María Velásquez‐Lopera, Ricardo Cardona,
... chronic spontaneous urticaria (CSU) that release eosinophil peroxidase (EPX) and eosinophil cationic protein (ECP). Continuous exposure to ... presence of IgE autoantibodies and cross-reactivity against EPX, ECP and thyroid peroxidase (TPO). The potential cross- ... in silico tools. RESULTS The frequencies of anti-EPX IgE (28.8%) and anti-ECP IgE (26. ... group, there was a correlation between the anti-EPX IgE and anti-TPO IgE levels (r = 0. ... 001); TPO inhibited 42% of IgE binding to EPX, while EPX inhibited 59% of IgE binding to ...
Tópico(s): Asthma and respiratory diseases
2021 - | Allergy Asthma and Immunology Research
Iftikar A. Saleem, Payman Sahbah Ahmed, Mayyadah S. Abed,
... the bullet. Aramid fabric (Kevlar) reinforced epoxy (KEV/EPX), or Ultra-high molecular weight polyethylene (UHMWPE) reinforced epoxy (UPE/EPX) represent the intermediate composites, whereas, the back layer ... agreement. The result shows that a SiC+UPE/EPX+Al-alloy and a SiC+KEV/EPX+Al-alloy were able to stop the 9 ... It is within the permissible range. SiC+UPE/EPX+Al-alloy absorbed more energy compared to SiC + KEV/EPX + Al-alloy. The SiC+UPE/EPX+Al-alloy, the ceramic layer absorbed more energy than the UPE/EPX and Al-alloy, reaching 80.5% of the ...
Tópico(s): Structural Response to Dynamic Loads
2021 - Elsevier BV | Materials Today Proceedings
Benjamin L. Wright, Alfred D. Doyle, Kelly P. Shim, Rish K. Pai, Suzanne Barshow, Jennifer L. Horsley‐Silva, Huijun Luo, Matthew A. Rank, Elizabeth A. Jacobsen, David A. Katzka, Hirohito Kita, Evan S. Dellon,
... requires manual quantification of tissue eosinophils. Eosinophil peroxidase (EPX) is an eosinophil-specific, cytoplasmic granule protein released ... this study was to evaluate image analysis of EPX immunohistochemistry as an automated method for histologic diagnosis ... were de-stained and re-stained to detect EPX for direct comparison. Slides were digitized, and EPX staining area/mm2 was quantified using image analysis. Paired samples were compared for changes in EPX staining in treatment responders and non-responders. Thirty- ...
Tópico(s): Eosinophilic Disorders and Syndromes
2020 - Springer Science+Business Media | Digestive Diseases and Sciences
Devyani Lal, Benjamin L. Wright, Kelly P. Shim, Matthew A. Zarka, James J. Lee, Yu‐Hui Chang, Sergei I. Ochkur, Rohit Divekar, Alfred D. Doyle, Elizabeth A. Jacobsen, Hirohito Kita, Matthew A. Rank,
... identify inflammatory targets and personalize therapy. Eosinophil peroxidase (EPX) is a biomarker for eosinophilic inflammation in patients ... TH1 inflammation. In this study, we hypothesized that EPX, and the GATA3:T-bet (G:T) ratio, ... results were studied further with immunohistochemical staining for EPX, GATA3, and T-bet. These methods and scoring ... algorithm was used to quantify the number of EPX-positive pixels. A nuclear algorithm was used to ... 002). Immunohistochemistry results for GATA3, T-bet, and EPX are presented in Table I; no significant difference ...
Tópico(s): Asthma and respiratory diseases
2019 - Elsevier BV | Journal of Allergy and Clinical Immunology
N. M. Hettiarachchi, Rangika Thilan De Silva, M.M.M.G.P.G. Mantilaka, Pooria Pasbakhsh, K.M. Nalin de Silva, G.A.J. Amaratunga,
... atomization. Herein, we synthesized a healing agent, epoxy (EPX) encapsulated calcium carbonate (CC) microcapsules, which was used to prepare self-healing EPX composites as a protective coating for metals. The ... respectively. The FTIR and NMR spectra confirmed the EPX encapsulation and the unreactive nature of the loaded EPX with the inner walls of CC microcapsules. The synthesized CC microcapsules were further incorporated into an EPX matrix to prepare composite coatings with 10 (w/ ... and 50 (w/w%) capsule loadings. The prepared EPX composite coatings were scratched and observed using FE- ...
Tópico(s): Carbon dioxide utilization in catalysis
2019 - Royal Society of Chemistry | RSC Advances
Caroline M. Percopo, Julia O. Krumholz, Elizabeth R. Fischer, Laura S Kraemer, Michelle Ma, Karen Laky, Helene F. Rosenberg,
Eosinophil peroxidase (EPX) is a major constituent of the large cytoplasmic granules of both human and mouse eosinophilic leukocytes. Human EPX deficiency is a rare, autosomal-recessive disorder limited ... Our intent was to explore the impact of EPX gene deletion on eosinophil content, structure, and function. ... eosinophils peripherally and in the respiratory tracts of EPX-/- mice compared to wild-type controls; furthermore, both ... and the smaller population of Gr1hi eosinophils from EPX-/- mice displayed lower median fluorescence intensities (MFIs) for ...
Tópico(s): Pediatric health and respiratory diseases
2018 - Oxford University Press | Journal of Leukocyte Biology
Zhixiong He, Lan Yang, Wenzhu Yang, K. A. Beauchemin, Shaoxun Tang, Jun Huang, C.S. Zhou, Xuefeng Han, M. Wang, Jinhe Kang, N. E. Odongo, Zhiliang Tan,
... evaluate varying enzyme products with high xylanase activity (EPX), four of which were recombinant single xylanase activity ... dry matter (DM). There were no interactions between EPX and dose for the fermentation characteristics evaluated. Increasing EPX dose linearly increased gas production (GP) kinetic characters [ ... 24 h was observed with increasing dose of EPX, but at 48 h only NDF degradability of ... increased. There were differences in the effects of EPX on degradability of DM and NDF from RS ... and EPX1 having the lowest. In addition, increasing EPX dose linearly increased acetate proportion at 24 h ...
Tópico(s): Phytase and its Applications
2014 - Cambridge University Press | The Journal of Agricultural Science
Sigurdur Kristjánsson, David Wennergren, Björn Eriksson, Halldóra Thórarinsdóttir, Göran Wennergren,
... High levels of urinary eosinophil protein X (U-EPX) have been related to active disease in asthmatic ... RSV bronchiolitis leads to an increase in U-EPX levels and whether wheezing is more common in children with high U-EPX values.Seventeen infants requiring in-ward care for ... up at mean age 32.9 months, U-EPX levels were comparable in the RSV group. However, ... RSV group had significantly increased levels of U-EPX compared with inclusion (median 167.8; range 46. ... subjects that experienced wheezing had significantly higher U-EPX values both at inclusion and at age 32. ...
Tópico(s): Asthma and respiratory diseases
2005 - Elsevier BV | Respiratory Medicine
Urinary eosinophilic protein X (U-EPX) measurement is easy to perform in children. However, its use for prediction, diagnosis, and monitoring of asthma and atopy is unclear.We sought to investigate the relationship between U-EPX and clinical phenotypes suggestive of allergic diseases.U-EPX measurement (RIA), respiratory questionnaires, and skin testing were ... with wheezing or eczema had slightly increased U-EPX levels compared with nonatopic asymptomatic children. U-EPX levels (geometric mean EPX/creatinine ratio) were as ...
Tópico(s): Occupational exposure and asthma
2003 - Elsevier BV | Journal of Allergy and Clinical Immunology
Neri Pucci, Enrico Lombardi, Elio Novembre, Silvia Fariña, Roberto Bernardini, Elisabetta Rossi, Tania Favilli, A Vierucci,
... Eosinophil cationic protein (ECP) and eosinophil protein X (EPX) or eosinophil-derived neurotoxin (EDN) are released by ... in adults and young patients, and high urinary EPX (u-EPX) levels in asthmatic patients seem to reflect active disease. A relationship between AD severity and u-EPX concentration in young children has not been previously ... children was correlated with s-ECP and u-EPX levels. Methods: Fifty-four infants and children (mean ... PBECs) were determined. In forty-two children u-EPX was also measured. Seven age-matched control patients ...
Tópico(s): Allergic Rhinitis and Sensitization
2000 - Elsevier BV | Journal of Allergy and Clinical Immunology
Vincent Cottin, P Deviller, Frédérique Tardy, Jean-François Cordier,
Background: Eosinophil-derived neurotoxin/protein X (EDN/EPX), one of the cationic granule proteins released by polymorphonuclear eosinophils, can be detected in human urine. Objective: We sought to evaluate whether the urinary release of EDN/EPX was dependent on the blood eosinophil cell count, ... corticosteroid treatment. Methods: Daily urinary release of EDN/EPX was measured by radioimmunoassay in 28 patients with ... group 4). Results: The urinary excretion of EDN/EPX was significantly higher in patients from groups 1 ...
Tópico(s): Eosinophilic Esophagitis
1998 - Elsevier BV | Journal of Allergy and Clinical Immunology
Sami Remes, Matti Korppi, Kyllikki Remes, Kari Savolainen, Ilkka Mononen, Juha Pekkanen,
... eosinophil cationic protein (ECP) and eosinophil protein X (EPX) in diagnosing asthma in children, and to investigate ... prick tests (SPT), on these markers. ECP and EPX were determined in 36 children with asthma, in ... therapy had significantly higher concentrations of ECP and EPX (ECP: 27.5 μg/L, P < 0.001; EPX: 59.9 μg/L, P < 0.001) than the control children (ECP: 11.2 μg/L; EPX: 26.2 μg/L). In the 20 children ... ECP: 13.6 μg/L, P < 0.01; EPX: 47.2 μg/L, P < 0.001) differed ... in the 166 control children. Both ECP and EPX had a significant association with allergic disorders and ...
Tópico(s): Respiratory and Cough-Related Research
1998 - Wiley | Pediatric Pulmonology
Claus M. Reimert, U. Minuva, A. Kharazmi, Klaus Bendtzen,
Eosinophil protein X/eosinophil derived neurotoxin (EPX/EDN) is one of the cationic proteins found in the granules of the human eosinophilic granulocytes. EPX was purified from extracts of granules isolated from ... buffy coat cells of healthy donors. Polyclonal anti-EPX antibodies were subsequently raised in rabbits. The anti-EPX-antibodies raised in rabbits showed no reactivity with ... utilized the biotin/avidin amplification system and measured EPX over the range of 60–2000 pg/ml. ... recoveries of 25 and 50 pg of purified EPX added to diluted serum samples were 106 ± 16% ( ...
Tópico(s): Antimicrobial Peptides and Activities
1991 - Elsevier BV | Journal of Immunological Methods
Hiba Hamdi, Imen Graiet, Salwa Abid‐Essefi, Joël Eyer,
Epoxiconazole (EPX), a widely used fungicide for domestic, medical, and industrial applications, could cause neurodegenerative diseases. However, the underling ... rats. Our results revealed that oral exposure to EPX at these concentrations (8, 24, 40, 56 mg/ ... fragmentation as assessed by the Comet assay. Indeed, EPX administration impaired activities of antioxidant enzymes and inhibited ... stem cells, we demonstrated that the treatment by EPX reduced the viability of cells with an IC50 ... also provoked the reduction of cell proliferation, and EPX triggered arrest in G1/S phase. The neurosphere ...
Tópico(s): Aluminum toxicity and tolerance in plants and animals
2021 - Elsevier BV | Chemosphere
Hiba Hamdi, Salwa Abid‐Essefi, Joël Eyer,
Epoxiconazole (EPX) is a very effective fungicide of the triazole family. Given its wide spectrum of use, the increased ... on human health. Previous studies have found that EPX is cytotoxic to cells, although the exact mechanism ... of action. We demonstrated that the treatment by EPX reduces the viability of cells in a dose ... It also provokes the reduction of cell proliferation. EPX could trigger arrest in G1/S phase of ... of F98 cells in G2/M phase. Moreover, EPX induced cytoskeleton disruption as evidenced by immunocytochemical analysis. ...
Tópico(s): Environmental Toxicology and Ecotoxicology
2019 - Elsevier BV | Chemosphere
Michael Wagner, Klas Sjöberg, Lina Vigren, Martin Olesen, Cecilia Benoni, Ervin Tóth, Marie Carlson,
... CC, elevated fecal levels of eosinophil protein X (EPX) and eosinophil cationic protein (ECP) have been reported. We aimed to evaluate if F-EPX, F-ECP, FC, and F-MPO could predict ... referred to colonoscopy. We also evaluated serum (S) EPX and ECP in this regard.Of 67 included ... 63 (94%) underwent colonoscopy with biopsy sampling. Fecal EPX, F-ECP, FC, F-MPO, S-EPX, and S-ECP were analyzed.Diagnostic outcome: normal: ... CD: n = 2 (3%). Higher levels of F-EPX and F-ECP were found in CC compared ... was 92%. We found no differences in S-EPX and S-ECP between the groups.Elevated F- ...
Tópico(s): Celiac Disease Research and Management
2016 - Taylor & Francis | Scandinavian Journal of Gastroenterology
Hillary Klonoff‐Cohen, Mounika Polavarapu,
... and interpretation of urine eosinophil protein X (u‐EPX) in clinical practice. Currently, there are no clear‐ ... appraised the epidemiological evidence to determine diagnostic u‐EPX cut points for pediatric asthma, and (2) performed a meta‐analysis to provide u‐EPX estimates for diagnosing pediatric asthma. Methods: Research articles ... of age were included. Both serum and urine EPX were included. Twenty‐seven studies met the inclusion ... nine studies for the meta‐analysis. Details regarding EPX analyses, treatment efficacy, and outcomes were assessed. For ...
Tópico(s): Eosinophilic Esophagitis
2016 - Wiley | Immunity Inflammation and Disease
Ole D. Wolthers, Carsten Heuck,
... ECP), and serum and urine eosinophil protein X (EPX) in children. Five girls and two boys aged ... samples for assessment of serum ECP and serum EPX were collected every 2 h during the 24 ... ECP (F=3.2, p=0.002), serum EPX (F=3.1, p=0.002) and in urine EPX/creatinine (F=5.4, p=0.003) were ... l) (p=0.01). Peak levels of serum EPX were seen at 06.00 (43.7 [9. ... l) (p=0.01). Peak levels of urine EPX/creatinine occurred in urine collected from 24.00 ... g/mmol) (p=0.02). Serum ECP, serum EPX and urine EPX exhibit a circadian variation in ...
Tópico(s): Allergic Rhinitis and Sensitization
2003 - Wiley | Pediatric Allergy and Immunology
Wassim Khalil Kalaajieh, Rémi Hoilat,
... the urinary concentration of eosinophil protein X (U-EPX) may objectively predict the severity and activity of asthma in children.Concentrations of U-EPX in 80 non- atopic asthmatic children were compared ... determined according to a pre-existing score. U-EPX was measured by the specific radioimmunoassay technique (Pharmacia, ... saturation, peak expiratory rate and eosinophil count.U-EPX concentrations were significantly higher in all asthmatic children ... Two weeks after resolution of the exacerbation, U-EPX significantly decreased (66.5 9.3 microg/mmol ...
Tópico(s): Allergic Rhinitis and Sensitization
2002 - Elsevier BV | Allergologia et Immunopathologia
A. Labbé, Bruno Aublet‐Cuvelier, Lionel Jouaville, G. Beaugeon, Lilia Fiani, Isabelle Petit, Lemlih Ouchchane, M. Doly,
... analyze sequential changes in urinary eosinophil protein X (EPX) a biological marker of eosinophil activation in asthmatic ... serum eosinophil cationic protein (s‐ECP) and urinary EPX assays. All subjects attended the outpatient clinic every ... following inclusion in this investigation. At baseline, urinary EPX concentrations were higher in children with asthma and ... between the AC and CC groups in urinary EPX and s‐ECP levels. s‐ECP concentrations were ... but no significant difference was observed for urinary EPX. Concentrations of urinary EPX were significantly correlated with ...
Tópico(s): Occupational exposure and asthma
2001 - Wiley | Pediatric Pulmonology
... been suggested that urinary eosinophil protein X (U-EPX) can be used to monitor bronchial inflammation in ... To determine the clinical value of measuring U-EPX in children with asthma and to evaluate the influence of atopy and airway infections, U-EPX was measured in 170 children with asthma (mean ... 1-165 months), and in 64 controls. U-EPX was elevated in children with acute asthma (median ... 39). Atopic children had higher levels of U-EPX than non-atopics with acute asthma (median 155 ... asthma, children without atopy had levels of U-EPX similar to values of controls; levels were similar ...
Tópico(s): Pediatric health and respiratory diseases
2001 - Wiley | Pediatric Allergy and Immunology
C Severien, A. Artlich, S. Jonas, G Becher,
... total body cysteinyl leukotriene production. Eosinophil protein X (EPX) has been used to assess eosinophil activity and ... to look for differences in urinary LTE4 and EPX concentrations between children with stable atopic asthma and ... the relationship was evaluated between urinary LTE4 and EPX levels and lung function. LTE4 was also measured (enzyme immunoassay) together with EPX (radioimmunoassay) in urine and lung function tests were ... pg.mg(-1) creatinine; p=0.021). Urinary EPX was also significantly increased in asthmatic children compared ...
Tópico(s): Pediatric health and respiratory diseases
2000 - European Respiratory Society | European Respiratory Journal
WOJNAROWSKI, ROITHNER, Köller, HALMERBAUER, Gartner, Tauber, FRISCHER,
... eosinophil cationic protein (ECP) and eosinophil protein X (EPX) may be valuable markers of airway inflammation in ... of inflammatory markers. Objective We measured ECP and EPX in nasal lavage fluids (NALF) and urine samples ... visit and analysed for ECP (NALF only) and EPX. Results Mean observation time was 186.4 days ( ... 1 and MEF 50 and nasal ECP, nasal EPX and urinary EPX were found. However, at exacerbations an average increase ... 9.3 vs 50.3 μg/L) and EPX (nasal EPX 36.4 vs 141.7 μg/L, urinary EPX 46.4 vs 74.1 μg/mmol creatinine) ...
Tópico(s): Allergic Rhinitis and Sensitization
1999 - Wiley | Clinical & Experimental Allergy
Storm Van'S Gravesande, Mattes, Grüntjens, Kopp, Seydewitz, Moseler, Kuehr,
... It is suggested that urinary eosinophil protein X (EPX) is a noninvasive tool to monitor bronchial inflammation ... and activation of eosinophils might possibly influence urinary EPX excretion. Objective Measurements of urinary EPX (radioimmunoassay) were used to investigate circadian variation of ... before and after treatment with corticosteroids. Methods Urinary EPX excretion (μg/mmol creatinine) was measured in the ... nonatopic, nonasthmatic controls to investigate circadian variation. Additionally, EPX excretion in the afternoon was analysed in 21 ...
Tópico(s): Chronic Obstructive Pulmonary Disease (COPD) Research
1999 - Wiley | Clinical & Experimental Allergy
Claus M. Reimert, John H. Ouma, Mariam T. Mwanje, Philip Magak, Lars K. Poulsen, Birgitte J. Vennervald, N. Ø. Christensen, Arsalan Kharazmi, Klaus Bendtzen,
... eosinophil cationic protein (ECP) and eosinophil protein X (EPX) in urine from Schistosoma haematobium-infected Kenyan school ... pathological changes as detected by ultrasonography. ECP and EPX were determined by means of specific ELISA methods ... children compared to controls, whereas high amounts of EPX were found in urine supernatants from infected children ... However, the amounts of cell bound ECP and EPX were significantly raised in infected children. In pre- ... by the amount of cell bound ECP and EPX, or ECP in urine supernatants. No such correlations ...
Tópico(s): Parasites and Host Interactions
1993 - Elsevier BV | Acta Tropica
Jorge Sánchez, Andres Sánchez Biol, Marlon Múnera Biol, Elizabeth García, Juan‐Felipe Lopez,
... of IgE and IgG autoantibodies against Eosinophil peroxidase (EPX) and Eosinophil cationic protein (ECP).Three steps were ... The frequency of IgE and IgG autoantibodies against EPX and ECP was investigated among asthmatic and healthy ... healthy subjects had IgE and IgG autoantibodies against EPX and ECP. Anti-EPX IgE was significantly higher in asthmatic patients. Severe ... age had IgE and IgG autoantibodies against to EPX and ECP. IgE autoantibodies to EPX and ECP induced basophil activation in asthmatic patients. ...
Tópico(s): Immune Cell Function and Interaction
2023 - Elsevier BV | World Allergy Organization Journal
Sergei I. Ochkur, Alfred D. Doyle, Elizabeth A. Jacobsen, William E. LeSuer, Wen Li, Cheryl Protheroe, Katie R. Zellner, Dana Colbert, Hua-Hao Shen, C. G. Irvin, James J. Lee, Nancy A. Lee,
... basic protein-1 (MBP-1) and eosinophil peroxidase (EPX), results in a near collapse of eosinophilopoiesis. The ... immune responses and pathologies occurring in MBP-1−/−/EPX−/− mice after 1) ovalbumin sensitization/provocation in an ... allergen-challenge protocol, and 2) crossing MBP-1−/−/EPX−/− mice with a double-transgenic model of chronic ... abolished in the absence of MBP-1 and EPX (i.e., MBP-1−/−/EPX−/− mice). The expression of MBP-1 and EPX was also required for induced lung expression of ... events and lung dysfunction. In summary, MBP-1−/−/EPX−/− mice provide yet another definitive example of the ...
Tópico(s): Eosinophilic Disorders and Syndromes
2017 - Oxford University Press | Journal of Leukocyte Biology