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Artigo Revisado por pares

Maurizio Franzini, Xiaocong M. Ye, Marc Adler, Danielle L. Aubele, Albert W. Garofalo, Shawn Gauby, Erich Goldbach, Gary D. Probst, Kevin P. Quinn, Pam Santiago, Hing L. Sham, Danny Tam, Vy Anh Truong, Zhao Ren,

Leucine-rich repeat kinase 2 (LRRK2) has been implicated in the pathogenesis of Parkinson's disease (PD). Inhibition of LRRK2 kinase activity is a therapeutic approach that may lead to new treatments for PD. Herein we report the discovery of a series of [1,2,4]triazolo[4,3-b]pyridazines that are potent against both wild-type and mutant LRRK2 kinase activity in biochemical assays and show an unprecedented selectivity towards the G2019S mutant. A structural rational for the observed selectivity is proposed.

Tópico(s): Signaling Pathways in Disease

2013 - Elsevier BV | Bioorganic & Medicinal Chemistry Letters

Artigo Acesso aberto Revisado por pares

Ilaria Ferrarotti, Gian Andri Thun, Michele Zorzetto, Stefania Ottaviani, Medea Imboden, Christian Schindler, Arnold von Eckardstein, Lucia Rohrer, Thierry Rochat, Erich W. Russi, Nicole Probst‐Hensch, Maurizio Luisetti,

Rationale α1-Antitrypsin (AAT) deficiency is one of the commonest rare respiratory disorders worldwide. Diagnosis, assessment of risk for developing chronic obstructive pulmonary disease (COPD), and management of replacement therapy require the availability of precise and updated ranges for protein serum levels. Objective This paper aims to provide ranges of serum AAT according to the main genotype classes in the general population. Methods The authors correlated mean AAT serum levels with the main SERPINA1 ...

Tópico(s): Calpain Protease Function and Regulation

2012 - BMJ | Thorax

Artigo Revisado por pares

Joachim Endell,

... 20 p. 396-396 Buchbesprechungen Bauchemie-Fibel. Von Erich Probst, Goslar (Harz). 4. Auflage, Carl Marhold, Verlagsbuchhandlung Halle ( ...

Tópico(s): Diatoms and Algae Research

1949 - Wiley | Chemie Ingenieur Technik

Artigo Acesso aberto Revisado por pares

Dennis Binder, Christopher Probst, Alexander Grünberger, Fabienne Hilgers, Anita Loeschcke, Karl‐Erich Jaeger, Dietrich Kohlheyer, Thomas Drepper,

Recombinant protein production is mostly realized with large-scale cultivations and monitored at the level of the entire population. Detailed knowledge of cell-to-cell variations with respect to cellular growth and product formation is limited, even though phenotypic heterogeneity may distinctly hamper overall production yields, especially for toxic or difficult-to-express proteins. Unraveling phenotypic heterogeneity is thus a key aspect in understanding and optimizing recombinant protein production ...

Tópico(s): Bacterial Genetics and Biotechnology

2016 - Public Library of Science | PLoS ONE

Artigo Acesso aberto Revisado por pares

Dennis Binder, Alexander Grünberger, Anita Loeschcke, Christopher Probst, Claus Bier, Jörg Pietruszka, Wolfgang Wiechert, Dietrich Kohlheyer, Karl‐Erich Jaeger, Thomas Drepper,

An optogenetic tool was established allowing for precise, gradual and homogeneous light-triggering of lac -based gene expression in a non-invasive fashion.

Tópico(s): Light effects on plants

2014 - Oxford University Press | Integrative Biology

Artigo Revisado por pares

Bruno Märkl, Therese Gannon Kerwel, Erich Langer, Wolfram Müller, Andreas Probst, Hanno Spatz, H Arnholdt,

Benign lesions in the gastrointestinal tract characterized by an increase of elastic fibers in the submucosal and mucosal layer are termed elastoma, elastosis, elastofibroma or elastofibromatous change, and present mostly as polyps. Twenty-seven such cases are published in the English and French literature. Some lesions are similar to alterations which are well-known from elastofibroma dorsi of the scapular region. The morphology is highly suggestive of amyloid, but the results of Congo red staining ...

Tópico(s): Heterotopic Ossification and Related Conditions

2008 - Elsevier BV | Pathology - Research and Practice

Artigo Acesso aberto Revisado por pares

Gian Andri Thun, Ilaria Ferrarotti, Medea Imboden, Thierry Rochat, Margaret W. Gerbase, Florian Kronenberg, Pierre-Olivier Bridevaux, Elisabeth Zemp, Michele Zorzetto, Stefania Ottaviani, Erich W. Russi, Maurizio Luisetti, Nicole Probst‐Hensch,

Background Severe alpha1-antitrypsin (AAT) deficiency is a strong risk factor for COPD. But the impact of gene variants resulting in mild or intermediate AAT deficiency on the longitudinal course of respiratory health remains controversial. There is indication from experimental studies that pro-inflammatory agents like cigarette smoke can interact with these variants and thus increase the risk of adverse respiratory health effects. Therefore, we tested the effect of the presence of a protease inhibitor ( ...

Tópico(s): Nutrition and Health in Aging

2012 - Public Library of Science | PLoS ONE

Artigo Revisado por pares

Christoph Kopp, Sabine Steiner, Ute Priglinger, Guenter Christ, Peter Probst, Gerald Maurer, Erich Minar, Kurt Huber,

A different rate and timing of subacute stent thrombosis after percutaneous coronary intervention was reported with various peri-interventional antithrombotic regimens. Next to platelet activation, coagulation triggered by tissue factor (TF) may play a key role in this process. Thirty-one consecutive patients with stable and unstable angina undergoing coronary stenting were randomly assigned to adjunct oral anticoagulation/anti-platelet therapy (coumadin, dipyridamole, aspirin and heparin; n = 16) or ...

Tópico(s): Atrial Fibrillation Management and Outcomes

2003 - Lippincott Williams & Wilkins | Blood Coagulation & Fibrinolysis

Artigo Revisado por pares

Hinrich Siefken,

... of Inge Aicher-Scholl, Anneliese Knoop-Graf, Michael Probst and Erich Schmorell. Gerhard Schott and Lilo Fürst-Ramdohr supplied ...

Tópico(s): Agricultural Development and Policies

1994 - Wiley | German Life and Letters

Artigo Revisado por pares

Albert W. Garofalo, Marc Adler, Danielle L. Aubele, Elizabeth Brigham, David Chian, Maurizio Franzini, Erich Goldbach, Grace Kwong, Ruth Motter, Gary D. Probst, Kevin P. Quinn, Lany Ruslim, Hing L. Sham, Danny Tam, Pearl Tanaka, Anh P. Truong, Xiaocong M. Ye, Zhao Ren,

Mutations in leucine-rich repeat kinase 2 (LRRK2) are associated with familial Parkinson's disease (PD). The kinase activity of this complex protein is increased by pathogenic mutations. Inhibition of LRRK2 kinase activity has therefore emerged as a promising approach for the treatment of PD. Herein we report our findings on a series of 4-alkylamino-7-aryl-3-cyanoquinolines that exhibit kinase inhibitory activity against both wild type and G2019S mutant LRRK2. Activity was determined in both biochemical ...

Tópico(s): Banana Cultivation and Research

2013 - Elsevier BV | Bioorganic & Medicinal Chemistry Letters

Artigo Acesso aberto Revisado por pares

Ivan Curjuric, Medea Imboden, Rachel Nadif, Ashish Kumar, Christian Schindler, Margot Haun, Florian Kronenberg, Nino Künzli, Harish C. Phuleria, Dirkje S. Postma, Erich W. Russi, Thierry Rochat, Florence Démenais, Nicole Probst‐Hensch,

Oxidative stress related genes modify the effects of ambient air pollution or tobacco smoking on lung function decline. The impact of interactions might be substantial, but previous studies mostly focused on main effects of single genes.We studied the interaction of both exposures with a broad set of oxidative-stress related candidate genes and pathways on lung function decline and contrasted interactions between exposures.For 12679 single nucleotide polymorphisms (SNPs), change in forced expiratory ...

Tópico(s): Climate Change and Health Impacts

2012 - Public Library of Science | PLoS ONE

Artigo Acesso aberto Revisado por pares

Medea Imboden, Joel Schwartz, Christian Schindler, Ivan Curjuric, Wolfgang Berger, Sally L.J. Liu, Erich W. Russi, Ursula Ackermann‐Liebrich, Thierry Rochat, Nicole Probst‐Hensch,

Decreasing exposure to airborne particulates was previously associated with reduced age-related decline in lung function. However, whether the benefit from improved air quality depends on genetic background is not known. Recent evidence points to the involvement of the genes p53 and p21 and of the cell cycle control gene cyclin D1 (CCND1) in the response of bronchial cells to air pollution.We determined in 4,326 participants of the Swiss Cohort Study on Air Pollution and Lung and Heart Diseases in ...

Tópico(s): Energy and Environment Impacts

2009 - National Institute of Environmental Health Sciences | Environmental Health Perspectives

Artigo Revisado por pares

D. Branscheid, S. Krysa, Erich Stoelben, H. Bülzebruck, G. Probst, I. Vogt‐Moykopf,

Die Resektion von Lungenmetastasen ist Bestandteil eines erfolgreichen interdisziplinären onkologichen Therapiekonzeptes. Von 1973 bis 1989 wurden bei 549 Patienten 620 Thorakotomien durchgeführt. Bei 66 Patienten handelte es sich um Metastasen eines Nierenzellkarzinoms. Die 30-Tage-Letalität liegt für alle Patienten bei 2,7 %, für die Patienten mit Nierenzellkarzinommetastasen bei 4,5 %. Die 5-Jahres-Überlebenszeit beträgt 37 %, bei radikaler Resektion 41 %. Die beste Langzeitprognose zeigen Patienten mit ...

Tópico(s): Renal and related cancers

1992 - Thieme Medical Publishers (Germany) | Aktuelle Urologie

Artigo Acesso aberto Revisado por pares

Judith Lang, Patrick Bohn, Hilal Bhat, Holger Jastrow, Bernd Walkenfort, Feyza Cansiz, Julian Fink, Michael Bauer, Dominik Olszewski, Ana Ramos-Nascimento, Vikas Duhan, Sarah-Kim Friedrich, Katrin Anne Becker, Adalbert Krawczyk, Michael J. Edwards, Andreas Burchert, Magdalena Huber, Justa Friebus‐Kardash, Joachim R. Göthert, Cornelia Hardt, Hans Christian Probst, Fabian Schumacher, Karl Köhrer, Burkhard Kleuser, Eduard B. Babiychuk, Beate Sodeik, Jürgen Seibel, Urs F. Greber, Philipp A. Lang, Erich Gulbins, Karl S. Lang,

Abstract Macrophages have important protective functions during infection with herpes simplex virus type 1 (HSV-1). However, molecular mechanisms that restrict viral propagation and protect from severe disease are unclear. Here we show that macrophages take up HSV-1 via endocytosis and transport the virions into multivesicular bodies (MVBs). In MVBs, acid ceramidase (aCDase) converts ceramide into sphingosine and increases the formation of sphingosine-rich intraluminal vesicles (ILVs). Once HSV-1 particles ...

Tópico(s): Research on Leishmaniasis Studies

2020 - Nature Portfolio | Nature Communications

Artigo Acesso aberto Revisado por pares

Byron A. Pirola, L Bortotto, Sabrina Giglio, Erich Piovan, A Janes, Renzo Guerrini, Orsetta Zuffardi,

Agenesis of the corpus callosum (ACC) is a relatively common brain abnormality resulting from developmental defects either limited to the structures leading to the proper formation of the corpus callosum or involving the embryo forebrain more generally. ACC is genetically heterogeneous with autosomal dominant, autosomal recessive, and X linked inheritance and has also been reported in subjects with aneuploidies involving several chromosomes. Among them, distal 6q deletions have been consistently ...

Tópico(s): Prenatal Screening and Diagnostics

1998 - BMJ | Journal of Medical Genetics

Artigo Acesso aberto Revisado por pares

Gerrit Haeger, Johanna Probst, Karl‐Erich Jaeger, Johannes Bongaerts, Petra Siegert,

Amino acid‐based surfactants are valuable compounds for cosmetic formulations. The chemical synthesis of acyl amino acids is conventionally performed by the Schotten–Baumann reaction using fatty acyl chlorides, but aminoacylases have also been investigated for use in biocatalytic synthesis with free fatty acids. Aminoacylases and their properties are diverse; they belong to different peptidase families and show differences in substrate specificity and biocatalytic potential. Bacterial aminoacylases ...

Tópico(s): Enzyme Structure and Function

2023 - Wiley | FEBS Open Bio

Artigo Revisado por pares

Volker Gekeler, Stefan Weger, Erich Eichele, Hans Christian Probst,

The newly designed equipment for alternating field gel electrophoresis which permits the separation of very large DNA molecules and the simultaneous analysis of up to 35 samples is described. The field alternation is effected by intermittently rotating the submerged agarose gel by optitional angles. The time intervals between changes of position are controlled by a computer program driving a simple switching device which was designed to suit any technique using periodic switching or inversion of ...

Tópico(s): Protein purification and stability

1989 - Elsevier BV | Analytical Biochemistry

Artigo Revisado por pares

Deniz Bilecen, Erich Seifritz, E. W. Radü, N. Schmid, Stephan G. Wetzel, Rudolf Probst, Klaus Scheffler,

Article abstract Unilateral acoustic stimulation produces a functional MRI (fMRI)–blood-oxygenation-level-dependent (BOLD) response mainly in the contralateral auditory cortex. In unilateral deaf patients, the BOLD response is bilateral. We studied a subject with sudden hearing loss after cochlear nerve resection before and repeatedly after surgery. During normal bilateral hearing, contralateral cortical BOLD responses were found. Progressing compensatory reorganization with bilateral representation ...

Tópico(s): Vestibular and auditory disorders

2000 - Lippincott Williams & Wilkins | Neurology

Artigo Acesso aberto Revisado por pares

Medea Imboden, Sara H. Downs, Oliver Senn, Gábor Mátyás, O Brändli, Erich W. Russi, Christian Schindler, Ursula Ackermann‐Liebrich, Wolfgang Berger, Nicole Probst‐Hensch,

Abstract Background Understanding the environmental and genetic risk factors of accelerated lung function decline in the general population is a first step in a prevention strategy against the worldwide increasing respiratory pathology of chronic obstructive pulmonary disease (COPD). Deficiency in antioxidative and detoxifying Glutathione S-transferase (GST) gene has been associated with poorer lung function in children, smokers and patients with respiratory diseases. In the present study, we assessed ...

Tópico(s): Epigenetics and DNA Methylation

2007 - BioMed Central | Respiratory Research

Artigo Revisado por pares

Amar Mehta, Gian Andri Thun, Medea Imboden, Ilaria Ferrarotti, Dirk Keidel, Nino Künzli, Hans Kromhout, David Miedinger, Harish C. Phuleria, Thierry Rochat, Erich W. Russi, Christian Schindler, Joel Schwartz, Roel Vermeulen, Maurizio Luisetti, Nicole Probst‐Hensch,

Objectives We evaluated interactions between SERPINA1 PiMZ genotype, associated with intermediate α1-antitrysin deficiency, with outdoor particulate matter ≤10 µm (PM 10 ), and occupational exposure to vapours, dusts, gases and fumes (VGDF), and their effects on annual change in lung function. Methods Pre-bronchodilator spirometry was performed in 3739 adults of the Swiss Cohort Study on Air Pollution and Lung Disease in Adults (SAPALDIA) for whom SERPINA1 genotypes were available. At baseline in 1991, participants ...

Tópico(s): Asthma and respiratory diseases

2013 - BMJ | Occupational and Environmental Medicine

Artigo Acesso aberto Revisado por pares

Elena Tello-García, Edoardo Mandolini, Erich Tasser, Maraike Probst, Ursula Peintner, Ulrike Tappeiner, Georg Leitinger,

Drought-induced changes in plant traits are well-studied, e.g. building smaller and thicker leaves, reducing stomatal density or increasing root uptake. However, the extent to which these changes depend on plant-soil interactions remains unclear. Mutualistic soil microbes like arbuscular mycorrhizal fungi (AMF) and Rhizobium are known to enhance plant productivity, water and nutrient uptake and stress tolerance. Therefore, alterations in soil microbial loads are expected to affect not only plant ...

Tópico(s): Agronomic Practices and Intercropping Systems

2023 - Elsevier BV | Environmental and Experimental Botany

Artigo Acesso aberto Revisado por pares

Ivan Curjuric, Medea Imboden, Christian Schindler, Sara H. Downs, Martin Hersberger, S. L. J. Liu, Gábor Mátyás, Erich W. Russi, Joel Schwartz, Gian Andri Thun, Dirkje S. Postma, Thierry Rochat, Nicole Probst‐Hensch,

Reduced exposure to particulate matter with a 50% cut-off aerodynamic diameter of 10 μm (PM 10 ) attenuated age-related lung function decline in our cohort, particularly in the small airways. We hypothesised that polymorphisms in glutathione S -transferase ( GST ) and haem oxygenase-1 ( HMOX1 ) genes, important for oxidative stress defence, modify these beneficial effects. A population-based sample of 4,365 adults was followed up after 11 yrs, including questionnaire, spirometry and DNA blood sampling. PM 10 ...

Tópico(s): Climate Change and Health Impacts

2010 - European Respiratory Society | European Respiratory Journal

Artigo Acesso aberto Revisado por pares

I. Curjuric, M. Imboden, Christian Schindler, S. H. Downs, M. Hersberger, S. L. J. Liu, G. Matyas, Erich W. Russi, Joel Schwartz, Gian Andri Thun, Dirkje S. Postma, Thierry Rochat, Nicole Probst‐Hensch,

Reduced exposure to particulate matter with a 50% cut-off aerodynamic diameter of 10 μm (PM 10 ) attenuated age-related lung function decline in our cohort, particularly in the small airways. We hypothesised that polymorphisms in glutathione S -transferase ( GST ) and haem oxygenase-1 ( HMOX1 ) genes, important for oxidative stress defence, modify these beneficial effects. A population-based sample of 4,365 adults was followed up after 11 yrs, including questionnaire, spirometry and DNA blood sampling. PM 10 ...

Tópico(s): Renal and related cancers

2009 - European Respiratory Society | European Respiratory Journal

Artigo Revisado por pares

Simeon Bowers, Gary D. Probst, Anh P. Truong, Roy K. Hom, Andrei W. Konradi, Hing L. Sham, Albert W. Garofalo, Karina Wong, Erich Goldbach, Kevin P. Quinn, John‐Michael Sauer, William H. Wallace, Lan K. Nguyen, Susanna S. Hemphill, Michael P. Bova, Guriqbal S. Basi,

The structural modification of a series of [3.3.1] bicyclic sulfonamide based gamma-secretase inhibitors is described. Appropriate substitution on the bicyclic scaffold provides a significant increase in the metabolic stability of the compounds resulting in an improved in vivo metabolic profile.

Tópico(s): Protein Kinase Regulation and GTPase Signaling

2009 - Elsevier BV | Bioorganic & Medicinal Chemistry Letters

Artigo Revisado por pares

Amar Mehta, David Miedinger, Dirk Keidel, Robert Bettschart, Andreas J. Bircher, Pierre-Olivier Bridevaux, Ivan Curjuric, Hans Kromhout, Thierry Rochat, Thomas Rothe, Erich W. Russi, Tamara Schikowski, Christian Schindler, Joel Schwartz, Alexander Turk, Roel Vermeulen, Nicole Probst‐Hensch, Nino Künzli,

Rationale: There is limited evidence from population-based studies demonstrating incidence of spirometric-defined chronic obstructive pulmonary disease (COPD) in association with occupational exposures.Objectives: We evaluated the association between occupational exposures and incidence of COPD in the Swiss Cohort Study on Air Pollution and Lung and Heart Diseases in Adults (SAPALDIA).Measurements and Main Results: Prebronchodilator ratio of forced expiratory volume in 1 second over forced vital capacity ( ...

Tópico(s): Chronic Obstructive Pulmonary Disease (COPD) Research

2012 - American Thoracic Society | American Journal of Respiratory and Critical Care Medicine

Artigo Acesso aberto Revisado por pares

Pierre-Olivier Bridevaux, Nicole Probst‐Hensch, Christian Schindler, Ivan Curjuric, Denise Felber Dietrich, Otto Braendli, Martin Brutsche, Luc Burdet, Manuel Frey, Margaret W. Gerbase, Ursula Ackermann‐Liebrich, Marco Pons, Jean‐Marie Tschopp, Thierry Rochat, Erich W. Russi,

The aim of the present study was to measure age-specific prevalence of airflow obstruction in Switzerland in smokers and never-smokers using pulmonary function tests and respiratory symptoms from 6,126 subjects participating in the Swiss Cohort Study on Air Pollution and Lung Diseases in Adults. The lower limit of normal of the forced expiratory volume in 1 s/forced vital capacity ratio was used to define airflow obstruction. Severity of airflow obstruction was graded according to the recommendations ...

Tópico(s): Injury Epidemiology and Prevention

2010 - European Respiratory Society | European Respiratory Journal