Matilde A. Rodrigues, Celina P. Leão, Eusébio P. Nunes, Sérgio Sousa, Pedro Arezes,
Organizations need to make decisions about risk acceptance, to decide about the need of risk-reducing measures. In this process, the personal judgments of occupational safety and health (OSH) practitioners have great importance. If on one hand, they have the technical knowledge about risk; on the other hand, the decisions can be dependent on their level of risk acceptance. This paper analyzes judgments of OSH practitioners about the level of risk acceptance, using the fuzzy logic approach. A questionnaire ...
Tópico(s): Multi-Criteria Decision Making
2016 - ASM International | ASCE-ASME Journal of Risk and Uncertainty in Engineering Systems Part B Mechanical Engineering
Nádia Eusébio, Raquel Castelo‐Branco, Diana Sousa, Marco Preto, Paul M. D’Agostino, Tobias A. M. Gulder, Pedro N. Leão,
Microginins are a large family of cyanobacterial lipopeptide protease inhibitors. A hybrid polyketide synthase/non-ribosomal peptide synthetase biosynthetic gene cluster (BGC) found in several microginin-producing strains─mic─was proposed to encode the production of microginins, based on bioinformatic analysis. Here, we explored a cyanobacterium, Microcystis aeruginosa LEGE 91341, which contains a mic BGC, to discover 12 new microginin variants. The new compounds contain uncommon amino acids, namely, ...
Tópico(s): Genomics and Phylogenetic Studies
2022 - American Chemical Society | ACS Synthetic Biology
Sofia Quental, Sandra Macedo‐Ribeiro, R M Albuquerque de Matos, Laura Vilarinho, Esmeralda Martins, Elisa Leão Teles, Esmeralda Rodrigues, Luísa Diogo, Paula Garcia, Filomena Eusébio, Ana Gaspar, Sílvia Sequeira, Fátima Furtado, Isabel Lança, António Amorim, Maria João Prata,
Maple syrup urine disease (MSUD) is an autosomal recessive disorder, caused by the defective function of the branched-chain alpha-ketoacid dehydrogenase complex (BCKD). BCKD is a mitochondrial complex, encoded by four nuclear genes (BCKDHA, BCKDHB, DBT and DLD), involved in the metabolism of branched-chain amino acids (BCAAs). Since the MSUD mutational spectrum has not been previously assessed in Portugal, in this study we present the molecular characterization of 30 MSUD Portuguese patients. Seventeen ...
Tópico(s): Blood disorders and treatments
2008 - Elsevier BV | Molecular Genetics and Metabolism
Nádia Eusébio, Adriana Rego, Nathaniel R. Glasser, Raquel Castelo‐Branco, Emily P. Balskus, Pedro N. Leão,
Abstract Background Halogenation is a recurring feature in natural products, especially those from marine organisms. The selectivity with which halogenating enzymes act on their substrates renders halogenases interesting targets for biocatalyst development. Recently, CylC – the first predicted dimetal-carboxylate halogenase to be characterized – was shown to regio- and stereoselectively install a chlorine atom onto an unactivated carbon center during cylindrocyclophane biosynthesis. Homologs of CylC ...
Tópico(s): Photosynthetic Processes and Mechanisms
2021 - BioMed Central | BMC Genomics
Sofia Quental, Laura Vilarinho, Esmeralda Martins, Elisa Leão Teles, Esmeralda Rodrigues, Luísa Diogo, P. de Diego Garciá, Filomena Eusébio, Ana Gaspar, Sílvia Sequeira, António Amorim, Maria João Prata,
Maple syrup urine disease is an autosomal recessive disorder of branched-chain amino acids metabolism with a worldwide frequency of 1/185,000 live newborns. In Portugal, the incidence of the disease has not been assessed. Based on the review of the cases diagnosed by tandem mass spectrometry an incidence of 1/86,800 live newborns was estimated in Portugal, indicating that the disease is more frequent in this country than reported in most populations.
Tópico(s): Neonatal Health and Biochemistry
2010 - Elsevier BV | Molecular Genetics and Metabolism