Produção Nacional
Revisado por pares
Bruna Pizziolo Coura, Jean Nunes dos Santos, Felipe Paiva Fonseca, Vanessa Fátima Bernardes, Sibele Nascimento de Aquino, Jacks Jorge Júnior, Pablo Agustín Vargas, Mário José Romañach, Bruno Augusto Benevenuto de Andrade, Ricardo Santiago Gomez, Carolina Cavaliéri Gomes,
Adenoid ameloblastoma is a rare epithelial neoplasm, histologically characterized by the presence of ameloblastoma-like features, duct-like structures, epithelial whorls, and cribriform architecture. Dentinoid material is usually present. Some advocate adenoid ameloblastoma is an ameloblastoma variant. However, there are overlapping features not only with ameloblastoma, but also with adenomatoid odontogenic tumor. Most ameloblastomas are characterized by the presence of BRAF p.V600E mutations and ...
Tópico(s): dental development and anomalies
2021 - Wiley | Journal of Oral Pathology and Medicine
Produção Nacional
Revisado por pares
Bruna Pizziolo Coura, Vanessa Fátima Bernardes, Sílvia Ferreira de Sousa, Marina Gonçalves Diniz, Rennan Garcias Moreira, Bruno Augusto Benevenuto de Andrade, Mário José Romañach, Hélder Antônio Rebelo Pontes, Ricardo Santiago Gomez, Edward Odell, Carolina Cavaliéri Gomes,
The molecular pathogenesis of mixed odontogenic tumors has not been established, and understanding their genetic basis could refine their classification and help define molecular markers for diagnostic purposes. Potentially pathogenic mutations in the component tissues of 28 cases of mixed odontogenic tumors were assessed. Laser capture microdissected tissue from 10 ameloblastic fibromas (AF), 4 ameloblastic fibrodentinomas (AFD), 6 ameloblastic fibro-odontomas (AFO), 3 ameloblastic fibrosarcomas (AFS), ...
Tópico(s): dental development and anomalies
2020 - Elsevier BV | Journal of Molecular Diagnostics
Produção Nacional
Revisado por pares
Bruna Pizziolo Coura, Vanessa Fátima Bernardes, Sílvia Ferreira de Sousa, Josiane Alves França, Núbia Braga Pereira, Hélder Antônio Rebelo Pontes, Aline Carvalho Batista, Danyel Elias da Cruz Pérez, Ricardo Luiz Cavalcanti de Albuquerque Júnior, Lélia Batista de Souza, Manoela Domingues Martins, Marina Gonçalves Diniz, Ricardo Santiago Gomez, Carolina Cavaliéri Gomes,
Adenomatoid odontogenic tumor is a benign encapsulated epithelial odontogenic tumor that shows an indolent clinical behavior. We have reported in a few adenomatoid odontogenic tumors mutations in KRAS, which is a proto-oncogene frequently mutated in cancer such as lung, pancreas, and colorectal adenocarcinomas. We aimed to assess KRAS mutations in the hotspot codons 12, 13, and 61 in a large cohort of adenomatoid odontogenic tumors and to test the association of these mutations with clinical (age, ...
Tópico(s): Bone Tumor Diagnosis and Treatments
2019 - Elsevier BV | Modern Pathology
Produção Nacional
Revisado por pares
Núbia Braga Pereira, Victor Coutinho Bastos, Juliana Cristina de Souza, Marina Gonçalves Diniz, Jéssica Gardone Vitório, Gregory T. Kitten, Luciana de Oliveira Andrade, Gleide Fernandes de Avelar, Wagner Henriques Castro, Vanessa Fátima Bernardes, Adriana Abalen Martins Dias, Ricardo Santiago Gomez, Carolina Cavaliéri Gomes,
Tópico(s): Head and Neck Surgical Oncology
2019 - Springer Nature | Clinical Oral Investigations
António O. L. Évora, Carlos E. S. Bernardes, M. Fátima M. Piedade, António C. L. Conceição, Manuel E. Minas da Piedade,
Multicomponent crystals have received significant attention in recent years, given their considerable potential to develop new products or improve the properties of known ones. Many studies have examined the synthesis and structure of multicomponent (mostly binary) crystals. The thermodynamic aspects related to the driving force for their formation and stability are, however, still relatively unexplored. This work describes a structure–energetics study of binary crystals consisting of glycine (Gly) ...
Tópico(s): Crystal structures of chemical compounds
2019 - American Chemical Society | Crystal Growth & Design
Produção Nacional
Revisado por pares
Andréia Souza Gonçalves, Diego Antônio Costa Arantes, Vanessa Fátima Bernardes, Filipe Jaeger, Janine Mayra da Silva, Tarcı́lia Aparecida Silva, Maria Cássia Ferreira de Aguiar, Aline Carvalho Batista,
The goal of this study was to compare the salivary concentrations of IL-10, TGF-β1 and soluble HLA-G (sHLA-G) in patients with oral squamous cell carcinoma (OSCC) to those in healthy individuals (control group), and to correlate the expression of these mediators in saliva with that in the tumour microenvironment. Neoplastic tissue and saliva samples from patients with OSCC (n = 22) were analysed by immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) respectively. We detected high expression ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2014 - Elsevier BV | Human Immunology
Produção Nacional
Revisado por pares
Vanessa Fátima Bernardes, Frederico O. Gleber‐Netto, Sílvia Ferreira de Sousa, Rafael Malagoli Rocha, Maria Cássia Ferreira de Aguiar,
To compare the immunohistochemistry (IHC) expression of epidermal growth factor receptor (EGFR) in oral squamous cell carcinomas (OSCC) with the gene amplification evaluated by fluorescence in situ hybridization (FISH) and chromogenic in situ hybridization (CISH) and their association with clinicopathological parameters. Additionally, we tested the sensibility and specificity of CISH in comparison with FISH.Case series studyOral surgery and pathology department in a school of dentistry.52 patients with ...
Tópico(s): Head and Neck Cancer Studies
2013 - BMJ | BMJ Open
Produção Nacional
Revisado por pares
Kelma Campos, Carolina Cavaliéri Gomes, Jeane de Fátima Correia‐Silva, Lucyana Conceição Farias, Thiago Fonseca–Silva, Vanessa Fátima Bernardes, Cláudia Maria Pereira, Ricardo Santiago Gomez,
Interferon-γ plays an important role in the pathogenesis of periapical lesions, and the methylation of IFNG has been associated with transcriptional inactivation. The purpose of the present study was to investigate IFNG promoter methylation in association with gene transcription and protein levels in periapical granulomas and radicular cysts.Methylation-specific polymerase chain reaction was used to assess the DNA methylation pattern of the IFNG gene in 16 periapical granulomas and 13 radicular ...
Tópico(s): Pancreatic and Hepatic Oncology Research
2013 - Elsevier BV | Journal of Endodontics
Produção Nacional
Revisado por pares
DC. Ribeiro, FO. Gleber-Netto, SF. Sousa, Vanessa Fátima Bernardes, MHN. Guimaraes-Abreu, MCF. Aguiar,
Objectives: to investigate the immunoexpression of epidermal growth factor receptor (EGFR) in a sample of oral leukoplakias (OL) and to determine the receptor's association with dysplasia, tobacco consumption, lesion site, and proliferation rate.Although EGFR should be overexpressed in some oral leukoplakias, the factors that may interfere with this expression and the influence of this receptor on epithelial proliferation have yet to be investigated.Study Design: Samples of oral leukoplakias (48) and ...
Tópico(s): Cancer and Skin Lesions
2012 - Medicina Oral S.L. | Medicina oral, patología oral y cirugía bucal
Produção Nacional
Revisado por pares
Lucyana Conceição Farias, Carolina Cavaliéri Gomes, João Artur Ricieri Brito, Clarice Ferreira Galvão, Marina Gonçalves Diniz, Wagner Henriques Castro, Vanessa Fátima Bernardes, Luiz Marco, Ricardo Santiago Gomez,
Ameloblastoma is a locally aggressive benign neoplasm derived from odontogenic epithelium, with high recurrence rates. Alterations in the Sonic Hedgehog signaling pathway, including PTCH gene mutations, have been associated with the pathogenesis of some odontogenic tumors. The purpose of the present study was to assess loss of heterozygosity at the PTCH locus in ameloblastoma. Twelve ameloblastomas were included, and loss of heterozygosity was assessed by using 3 microsatellite markers D9S252, D9S127, ...
Tópico(s): dental development and anomalies
2012 - Elsevier BV | Human Pathology
Produção Nacional
Revisado por pares
Carolina Cavaliéri Gomes, Marina Gonçalves Diniz, Alessandra Pires Duarte, Vanessa Fátima Bernardes, Ricardo Santiago Gomez,
Odontogenic myxoma (OM) is a benign odontogenic neoplasm that tends to recur due to bone infiltration. This review focuses on the molecular aspects of the OM. The following topics are discussed: clonal nature, matrix metalloproteinases, apoptosis and cell proliferation, genetic alterations, and other markers. Translational studies are necessary to identify the prognostic markers of this lesion, and also, molecular biology studies may help to identify the etiologic factors and to develop more effective ...
Tópico(s): Cardiac tumors and thrombi
2011 - Elsevier BV | Oral Oncology
Produção Nacional
Revisado por pares
Fabrício TA Souza, Tálita PM Santos, Vanessa Fátima Bernardes, Antônio Lúcio Teixeira, Arthur Kümmer, Tarcı́lia Aparecida Silva, Mauro HNG Abreu,
Abstract Background Burning mouth syndrome is a chronic disorder that is characterized by a burning sensation and a normal clinical appearance of the oral mucosa. This condition often affects the health-related quality of life in patients. As such, the aim of this study was to compare the health-related quality of life of patients with BMS and healthy controls, using the validated Portuguese versions of the SF-36 and OHIP-49 questionnaires. Methods A calculated sample of Brazilian patients with ...
Tópico(s): Psoriasis: Treatment and Pathogenesis
2011 - BioMed Central | Health and Quality of Life Outcomes
Produção Nacional
Revisado por pares
Fabrício Rezende Amaral, Vanessa Fátima Bernardes, Alessandra Pires Duarte, Núbia Braga Pereira, A.C. Vasconcelos, Ricardo Santiago Gomez, Carolina Cavaliéri Gomes,
Central giant cell lesion (CGCL) and peripheral giant cell lesion (PGCL) of the jaws are characterized by multinucleated osteoclast-like giant cells in a background of mononuclear cells. While mononuclear cells retain proliferative activity in both lesions, giant cells are Ki-67 negative. This observation raised the theory that giant cells are formed by cytoplasmic fusion of mononuclear cells, and also that these lesions are of reactive nature. As the giant cells are not proliferating in CGCL and ...
Tópico(s): Cell death mechanisms and regulation
2011 - SAGE Publishing | Tumor Biology
Produção Nacional
Revisado por pares
Vanessa Fátima Bernardes, Frederico O. Gleber‐Netto, Sílvia Ferreira de Sousa, Tarcı́lia Aparecida Silva, Mauro Henrique Nogueira Guimarães de Abreu, Maria Cássia Ferreira de Aguiar,
The objective of this research was to investigate the salivary levels of epidermal growth factor (EGF) in patients with oral squamous cell carcinoma (OSCC) in comparison with clinically healthy individuals and to verify the immunoexpression of EGF in tumor samples. In addition, the relationship between salivary levels and tumoral EGF expression with clinicopathologic features was investigated. We carried out an investigation on EGF expression in lesion samples and in saliva of OSCC patients through ...
Tópico(s): Head and Neck Cancer Studies
2011 - Lippincott Williams & Wilkins | Applied immunohistochemistry & molecular morphology
Carlos E. S. Bernardes, M. Fátima M. Piedade, Manuel E. Minas da Piedade,
A new polymorph of 4′-hydroxyacetophenone (form I, monoclinic, P21/c, Z′ = 1) was isolated and characterized. The structural differences between this phase and the previously known one (form II, orthorhombic, P212121, Z′ = 2) were investigated by X-ray diffraction. The two polymorphs exhibit distinct packing features and, at the molecular level, they seem to differ by the relative conformations of the OH and C(O)CH3 groups. The stability domains of the two phases from 298.15 K to the fusion temperature ...
Tópico(s): Crystallization and Solubility Studies
2008 - American Chemical Society | Crystal Growth & Design
Produção Nacional
Revisado por pares
Sérgio de Melo Alves Júnior, Sérgio Vitorino Cardoso, Vanessa Fátima Bernardes, Vinícius de Carvalho Machado, Ricardo Alves Mesquita, María Auxiliadora Vieira do Carmo, Maria Cássia Ferreira de Aguiar,
Tópico(s): Head and Neck Anomalies
2006 - Elsevier BV | Oral Oncology
Produção Nacional
Revisado por pares
Vanessa Fátima Bernardes, Frederico O. Gleber‐Netto, Sílvia Ferreira de Sousa, Tarcı́lia Aparecida Silva, Maria Cássia Ferreira de Aguiar,
Abstract Background The erbB receptors and their ligands are involved in the pathogenesis and progression of oral squamous cell carcinoma (OSCC). Although EGFR and Her-2 are frequently overexpressed in OSCC, few studies evaluated these proteins in saliva and their association with the tumor, which may represent potential usefulness in a clinical setting. Methods The levels of EGFR, Her-2, and EGF were evaluated in saliva of 46 patients with OSCC before and after the surgical removal of the lesion, ...
Tópico(s): Neuroblastoma Research and Treatments
2010 - BioMed Central | Journal of Experimental & Clinical Cancer Research
Produção Nacional
Revisado por pares
Vanessa Fátima Bernardes, Júlio César Tanos de Lacerda, Maria Cássia Ferreira de Aguiar, Ricardo Santiago Gomez,
Odontogenic tumors composed of two or more distinct types of lesions are unusual. In this paper, a case of an odontogenic lesion characterized by simultaneous occurrence of areas of calcifying odontogenic cyst (COC) and orthokeratinized odontogenic cyst (OOC) is described. The lesion was asymptomatic and presented at the radiographic examination as a unilocular well-delimited radiolucency extending from left incisor to right premolar area in the mandible. To date, this is the first report of COC associated ...
Tópico(s): Vascular Malformations and Hemangiomas
2008 - Springer Science+Business Media | Head and Neck Pathology
Produção Nacional
Revisado por pares
Carolina Cavaliéri Gomes, Vanessa Fátima Bernardes, Marina Gonçalves Diniz, Luiz Marco, Ricardo Santiago Gomez,
Development of accurate therapeutic approaches to salivary gland neoplasms depends on better understanding of their molecular pathogenesis. Tumour growth is regulated by the balance between proliferation and apoptosis. Few studies have investigated apoptosis in salivary tumours relying almost exclusively on immunohistochemistry or TUNEL assay. Furthermore, there is no information regarding the mRNA expression profile of apoptotic genes in salivary tumors. Our objective was to investigate the quantitative ...
Tópico(s): Neuroblastoma Research and Treatments
2012 - BioMed Central | BMC Cancer
Produção Nacional
Revisado por pares
Abhinav Joseph, Joana S. Rodrigues Alves, Carlos E. S. Bernardes, M. Fátima M. Piedade, Manuel E. Minas da Piedade,
Different 5-hydroxynicotinic acid tautomers were selectively captured through solvate formation. The selectivity is lost once the memory of solvation is erased by removing the solvent from the crystal lattice.
Tópico(s): Chemical Synthesis and Analysis
2019 - Royal Society of Chemistry | CrystEngComm
Produção Nacional
Revisado por pares
Helena de Fátima Bernardes Millani, Marialuisa Valente,
El texto parte de nuevos conceptos y prácticas de la psiquiatría que atraviesan el contexto de la salud mental colectiva, considerando el desarrollo histórico que favorece el entendimiento para la construcción de nuevos maneras sociales y técnicas en el lidiar con la locura, la enfermedad mental y el sufrimiento humano. También destaca que la atención al enfermo mental pasó por varios procesos de transformación, cambiando de la institucionalización para los Servicios de Atención Psicosocial, realizando ...
Tópico(s): Youth, Drugs, and Violence
2008 - | SMAD Revista Eletrônica Saúde Mental Álcool e Drogas (Edição em Português)
Produção Nacional
Revisado por pares
Josiane Alves França, Sílvia Ferreira de Sousa, Rennan Garcias Moreira, Vanessa Fátima Bernardes, Letícia Martins Guimarães, Jean Nunes dos Santos, Marina Gonçalves Diniz, Ricardo Santiago Gomez, Carolina Cavaliéri Gomes,
Granular cell tumour (GCT) is a benign nerve sheath neoplasm of unknown molecular pathogenesis. Although a skeletal muscle cell origin was initially proposed for GCT, its neural origin, derived from Schwann cells, is supported by S-100 immunopositivity.1 There are few molecular studies on oral GCT.2 GCTs have been previously described in patients with LEOPARD and Noonan syndrome with PTPN11 gene mutations,3–6 as well as in a patient with PTEN hamartoma tumour syndrome with PTEN mutation.7 Therefore, ...
Tópico(s): Cancer Diagnosis and Treatment
2017 - BMJ | Journal of Clinical Pathology
Carlos E. S. Bernardes, M. Fátima M. Piedade, Manuel E. Minas da Piedade,
A new hydrate of 4′-hydroxyacetophenone (HAP), triclinic, space group P1̅, was isolated and characterized. Both the structure determination by single crystal X-ray diffraction at 150 K and the results of thermogravimetry analysis carried out in the temperature range 263−363 K indicated the H2O/HAP molar ratio to be 1.5. The water molecules line up in chains that reside in lattice channels (channel hydrate) and are sustained by OH···O hydrogen bonds. The chains also interact with the OH (donor) and ...
Tópico(s): Chemical and Physical Properties in Aqueous Solutions
2010 - American Chemical Society | Crystal Growth & Design
Produção Nacional
Revisado por pares
Filipe Nobre Chaves, Thâmara Manoela Marinho Bezerra, Débora Chaves Moraes, Sara Ferreira dos Santos Costa, Paulo Goberlanio Barros Silva, Ana Paula Negreiros Nunes Alves, Fábio Wildson Gurgel Costa, Vanessa Fátima Bernardes, Karuza Maria Alves Pereira,
Oral epithelial dysplasia (OED) is a risk factor for developing subsequent oral squamous cell carcinoma (OSCC). Loss of heterozygosity (LOH) profiles have been validated as risk predictors of malignant transformation of OED. It is still unclear if Phosphatase and tensin homologue deleted on chromosome 10 (PTEN) allelic loss also occurs in initial stage malignant lesions and if the allelic loss is involved as one of the mechanisms of oral carcinogenesis. Thus, this study objective investigate LOH of ...
Tópico(s): Oral and gingival health research
2019 - Elsevier BV | Experimental and Molecular Pathology
Produção Nacional
Revisado por pares
Josiane Alves França, Marina Gonçalves Diniz, Vanessa Fátima Bernardes, Raíssa Cristina Costa‐Silva, Renan P. Souza, Ricardo Santiago Gomez, Carolina Cavaliéri Gomes,
Background Cohesin complex is responsible for sister chromatid cohesion. STAG1/STAG2 is part of the complex, which is regulated by PDS5B . Alterations in these genes were described in tumors. PDS5B is a negative regulator of cell proliferation. We aimed to assess molecular alterations in these genes in oral squamous cell carcinoma (OSCC) and predict their expression by the expression of 84 cell cycle genes. In addition, we investigated whether pds5b protein expression impacted ki‐67 and p53 immunopositivity. ...
Tópico(s): Ubiquitin and proteasome pathways
2016 - Wiley | Journal of Oral Pathology and Medicine
Produção Nacional
Revisado por pares
Vanessa Fátima Bernardes, Marina Gonçalves Diniz, Jéssica Carolina Silva, Débora Chaves Moraes, Luiz Marco, Carolina Cavaliéri Gomes, Ricardo Santiago Gomez,
Chronic mucosal trauma is suggested as an additional etiologic risk factor for oral squamous cell carcinoma (OSCC), but there is a lack of experimental-molecular data. If chronic trauma of the oral mucosa is carcinogenic, it should be associated with early genetic alterations seen during typical progression of OSCC, like loss of heterozygosity (LOH). We investigated LOH in the key chromosomal arms 3p, 9p and 17p in inflammatory fibrous hyperplasia associated with removable dental prosthesis and also ...
Tópico(s): Oral and Maxillofacial Pathology
2019 - Wiley | Journal of Oral Pathology and Medicine
Produção Nacional
Revisado por pares
Lucyene Miguita, Juliana Cristina de Souza, Victor Coutinho Bastos, Núbia Braga Pereira, Raphaela Alvarenga Braga de Freitas, Letícia Martins Guimarães, Gleide Fernandes de Avelar, Luciana Oliveira Andrade, Walderez O. Dutra, Fábio Daumas Nunes, Wagner Henriques de Castro, Júlio César Tanos de Lacerda, Amanda Maria Sena Reis, Vanessa Fátima Bernardes, Marina Gonçalves Diniz, Ricardo Santiago Gomez, Carolina Cavaliéri Gomes,
Central giant cell granulomas (CGCG) of the jaws are osteolytic lesions that may behave aggressively and respond poorly to surgery. Microscopically, in addition to giant cells, there is a mononuclear cell population composed of macrophage/monocytic cells and spindle-shaped cells of mesenchymal origin. Seventy two percent of these tumours harbour mutually exclusive TRPV4, KRAS and FGFR1 mutations. We aimed to assess the mutational status of mononuclear and giant cells and the osteogenic potential ...
Tópico(s): Sarcoma Diagnosis and Treatment
2022 - Wiley | Journal of Oral Pathology and Medicine
Produção Nacional
Revisado por pares
Vanessa Fátima Bernardes, Edward Odell, Ricardo Santiago Gomez, Carolina Cavaliéri Gomes,
Chromosomal instability, leading to aneuploidy, is one of the hallmarks of human cancers. USP44 (ubiquitin specific peptidase 44) is an important molecule that plays a regulatory role in the mitotic checkpoint and USP44 loss causes chromosome mis-segregation, aneuploidy and tumorigenesis in vivo. In this study, it was investigated the immunoexpression of USP44 in 28 malignant salivary gland neoplasms and associated the results with DNA ploidy status assessed by image cytometry. USP44 protein was ...
Tópico(s): Salivary Gland Tumors Diagnosis and Treatment
2017 - Fundação Odontológica de Ribeirão Preto | Brazilian Dental Journal
Produção Nacional
Revisado por pares
Gefter Thiago Batista Corrêa, Vanessa Fátima Bernardes, Sílvia Ferreira de Sousa, Marina Gonçalves Diniz, José Maria Porcaro Salles, Renan P. Souza, Alfredo Maurício Batista de Paula, Ricardo Santiago Gomez, Carolina Cavaliéri Gomes,
Molecular mechanisms of lip squamous cell carcinoma (LSCC) and actinic cheilitis (AC) are unclear. We aimed at assessing loss of heterozygosity (LOH) and TP53 and BRAF V600E mutations in these lesions. Formalin-fixed paraffin-embedded (FFPE) samples of 17 LSCC and 16 AC were included, with additional 5 fresh LSCC genotyped for TP53 mutations. LOH was assessed by six polymorphic markers located at 9p22, 9q22, and 17p13 and correlated with cell proliferation (Ki-67) and P53 immunostaining. Direct sequencing ...
Tópico(s): Nonmelanoma Skin Cancer Studies
2015 - SAGE Publishing | Tumor Biology
Ricardo G. Simões, Carlos E. S. Bernardes, Abhinav Joseph, M. Fátima M. Piedade, Werner Kraus, Franziska Emmerling, Hermı́nio P. Diogo, Manuel E. Minas da Piedade,
Simvastatin is one of the most widely used active pharmaceutical ingredients for the treatment of hyperlipidemias. Because the compound is employed as a solid in drug formulations, particular attention should be given to the characterization of different polymorphs, their stability domains, and the nature of the phase transitions that relate them. In this work, the phase transitions delimiting the stability domains of three previously reported simvastatin forms were investigated from structural, ...
Tópico(s): Computational Drug Discovery Methods
2018 - American Chemical Society | Molecular Pharmaceutics