
I.B Santos, Rogério Tortelly, Leonardo Pereira Quintella, Maria de Fátima Madeira, Luisa Helena Monteiro de Miranda, Fabiano Borges Figueiredo, Raquel de Vasconcellos Carvalhães de Oliveira, Tânia Maria Pacheco Schubach,
... this author on: Oxford Academic PubMed Google Scholar Fabiano Borges Figueiredo, Fabiano Borges Figueiredo eServiço de Dermatozoonoses, Instituto de Pesquisa Clínica ...
Tópico(s): T-cell and Retrovirus Studies
2015 - Oxford University Press | Transactions of the Royal Society of Tropical Medicine and Hygiene

Edvar Yuri Paheco Schubach, Fabiano Borges Figueiredo, Gustavo Adolfo Sierra Romero,
Visceral leishmaniasis is a major public health concern in Brazil and the domestic dog is the main source of infection. In this study, we aimed to evaluate the accuracy and reliability of a rapid chromatographic immunoassay based on a dual-path platform for the diagnosis of canine visceral leishmaniasis (CVL). Sampling consisted of 428 domestic dogs selected from two neighborhoods in the municipality of Fortaleza, Ceara state, Brazil. The reference standard was composed of three parasitological tests ...
Tópico(s): Parasites and Host Interactions
2014 - Oxford University Press | Transactions of the Royal Society of Tropical Medicine and Hygiene

Cíntia Xavier de Mello, Fabiano Borges Figueiredo, Artur Augusto Velho Mendes, Marina Carvalho Furtado, Luciana Freitas Campos de Miranda, Maria de Fátima Madeira,
Introduction In Brazil, visceral leishmaniasis (VL) has spread to various regions. This study reports canine cases of VL in Barra Mansa, where human VL cases were recently reported. Methods Using the human index case, a canine survey was performed by dual-path platform immunochromatography and enzyme-linked immunosorbent assay. Seropositive animals were euthanized. Cultures were collected to detect Leishmania parasites. Results Serological tests detected 141 canine VL cases, and Leishmania chagasi ...
Tópico(s): Research on Leishmaniasis Studies
2014 - Brazilian Society of Tropical Medicine | Revista da Sociedade Brasileira de Medicina Tropical

Arleana B.P.F. Almeida, Valéria Régia Franco Sousa, Naiani Domingos Gasparetto, Givago F.R. da Silva, Fabiano Borges Figueiredo, Valéria Dutra, Luciano Nakazato, Maria F. Madeira,
The accurate diagnosis of canine visceral leishmaniasis (CanL) is essential for visceral leishmaniasis control. To this end, DNA detection on different biological samples has been employed. In this study, we report the use of polymerase chain reaction (PCR) assay on samples such as buffy coat, bone marrow, intact skin and cutaneous ulcers fragments, and lymph node aspirate collected from 430 dogs to determine the suitable biological sample for use in CanL diagnosis. The PCR results were correlated ...
Tópico(s): Leptospirosis research and findings
2013 - Elsevier BV | Diagnostic Microbiology and Infectious Disease

Carla O Honse, Fabiano Borges Figueiredo, Nayro Xavier de Alencar, Maria de Fátima Madeira, Isabella DF Gremião, T. M. P. Schubach,
Disseminated intravascular coagulation (DIC) is an acquired disorder characterized by the activation of intravascular coagulation and excessive fibrin formation. It always occurs in association with other clinical conditions, including parasitic diseases. DIC has been described as a unusual complication in human and canine visceral leishmaniasis.DIC was found in a seven-year-old male mongrel dog naturally infected by Leishmania (Leishmania) chagasi. Haemostasis parameters demonstrated changes in primary ...
Tópico(s): Trypanosoma species research and implications
2013 - BioMed Central | BMC Veterinary Research

M.F. Madeira, Fabiano Borges Figueiredo, A.G.S. Pinto, Lílian Dias Nascimento, Marina Carvalho Furtado, Eliame Mouta‐Confort, Cíntia Cristiane de Paula, Alessandra Bogio, Marta C. Gomes, A.M.S. Bessa, Sônia Regina Lambert Passos,
The objective of this study was to evaluate intact skin of seroreactive dogs as a possible target for the parasitological confirmation of canine visceral leishmaniasis (CVL). For this purpose, 394 dogs identified in serological surveys carried out in the metropolitan region of Belo Horizonte were studied. Blood was collected from all animals for serology and a tissue sample was obtained from two sites for parasitological diagnosis. Skin obtained from the ear and scapular region was simultaneously ...
Tópico(s): Vector-borne infectious diseases
2009 - Elsevier BV | Research in Veterinary Science

M.F. Madeira, Maria Alice Pereira de Sousa, Juliana H. S. Barros, Fabiano Borges Figueiredo, Aline Fagundes, A Schubach, Cíntia Cristiane de Paula, B. N. S. Faissal, Tatiana S. Fonseca, H. K. Thoma, M. C. A. Marzochi,
SUMMARY An unknown Trypanosoma species was isolated from an axenic culture of intact skin from a domestic dog captured in Rio de Janeiro, Brazil, which was co-infected with Leishmania ( Viannia ) braziliensis . Giemsa-stained smears of cultures grown in different media revealed the presence of epimastigotes, trypomastigotes, spheromastigotes, transitional stages, and dividing forms (epimastigotes or spheromastigotes). The highest frequency of trypomastigotes was observed in RPMI (15·2%) and DMEM (9·2%) media ...
Tópico(s): Toxoplasma gondii Research Studies
2009 - Cambridge University Press | Parasitology

Luisa Helena Monteiro de Miranda, Leonardo Pereira Quintella, I.B Santos, Rodrigo Caldas Menezes, Fabiano Borges Figueiredo, Isabella Dib Ferreira Gremião, Thais Okamoto, Raquel de Vasconcellos Carvalhães de Oliveira, Sandro Antônio Pereira, Rogério Tortelly, T. M. P. Schubach,
Tópico(s): Infectious Diseases and Mycology
2009 - Springer Science+Business Media | Mycopathologia

Cíntia Cristiane de Paula, Fabiano Borges Figueiredo, Rodrigo Caldas Menezes, Eliame Mouta‐Confort, Alessandra Bogio, Maria de Fátima Madeira,
Leishmaniose visceral é uma zoonose de importância em Saúde Pública, onde os cães representam um dos maiores problemas. Este trabalho visa relatar o primeiro caso autóctone da leishmaniose visceral canina no município de Maricá, fornecendo elementos relacionados à distribuição geográfica de Leishmania (Leishmania) chagasi no Estado do Rio de Janeiro.
Tópico(s): Trypanosoma species research and implications
2009 - Brazilian Society of Tropical Medicine | Revista da Sociedade Brasileira de Medicina Tropical

Fabiano Borges Figueiredo, Isabel Cristina Fábregas Bonna, Lílian Dias Nascimento, Tatiana da Costa, Cibele Baptista, Tânia Maria Valente Pacheco, Maria Regina Reis Amendoeira, Maria de Fátima Madeira,
A partir de um caso canino de leishmaniose tegumentar americana na localidade de Santa Rita de Cássia, município de Barra Mansa, Rio de Janeiro, foi realizado um inquérito sorológico em 177 cães e 43 gatos. Na avaliação das amostras de soros caninos, 10% foram positivos na reação de imunofluorescência indireta e 10,7% no ensaio imunoenzimático. Entre as amostras de soros felinos testados, nenhum animal foi positivo na reação de imunofluorescência indireta e apenas um (2,4%) felino apresentou reação ...
Tópico(s): Trypanosoma species research and implications
2009 - Brazilian Society of Tropical Medicine | Revista da Sociedade Brasileira de Medicina Tropical

Fabiano Borges Figueiredo, M.F. Madeira, Rodrigo Caldas Menezes, R. S. Pacheco, M.Q. Pires, Marina Carvalho Furtado, A.G.S. Pinto, T. M. P. Schubach,
Of 146 dogs from a visceral leishmaniosis-endemic area that tested seronegative by indirect immunofluorescence (IIF) on blood samples collected on filter paper (IIFp), 51 (34.9%) and 10 (6.8%) tested positive by IIF on serum samples (IIFs) and enzyme immunoassay, respectively. Three samples (2.0%) tested positive by PCR. Leishmania chagasi was isolated from the skin of five (3.4%) dogs. Amastigote forms were identified in two of these five animals following histopathological and immunohistochemical examination. ...
Tópico(s): Insect and Pesticide Research
2009 - Elsevier BV | The Veterinary Journal

Cáthia Maria Barrientos Serra, Cristianni Antunes Leal, Fabiano Borges Figueiredo, T. M. P. Schubach, Rosemere Duarte, Cláudia Maria Antunes Uchôa, Roger Magno M. Silva, Maria de Fátima Madeira,
Descreve-se a ocorrência da leishmaniose tegumentar em cães da localidade de Morada das Águias (Serra da Tiririca), Maricá, Estado do Rio de Janeiro, Brasil. Foram avaliados 83 cães por meio de exames clínico, sorológico e parasitológico. Os soros de 11 (13,2%) animais foram reagentes à imunofluorescência indireta (IFI) e de 30 (36,1%) ao ensaio imunoenzimático (ELISA). Úlceras cutâneas e ou mucosas foram observadas em 18 (n = 83; 21,7%) dos animais. Leishmania foi isolada de 11 cães. Discute-se a ocorrência ...
Tópico(s): Research on Leishmaniasis Studies
2003 - Escola Nacional de Saúde Pública, Fundação Oswaldo Cruz | Cadernos de Saúde Pública

Andressa Guimarães de Souza Pinto, T. M. P. Schubach, Fabiano Borges Figueiredo, C. Baptista, Aline Fagundes, Juliana Helena da Silva Barros, Cíntia Cristiane de Paula, Helena Keiko Toma, M.F. Madeira,
SUMMARY The domestic dog's involvement with different members of the Trypanosomatidae family has been the focus of several studies due to this animal's close proximity to man. Recently this animal has been infected by a new Trypanosoma species ( T. caninum ), described in Rio de Janeiro and 19 similar isolates were later obtained. The objective of this study was to identify these isolates. All samples were isolated from intact skin cultures and analysed morphologically, by biochemical isoenzyme electrophoresis ...
Tópico(s): Parasitic Diseases Research and Treatment
2010 - Cambridge University Press | Parasitology

Sandro Antônio Pereira, Rodrigo Caldas Menezes, Isabella Dib Ferreira Gremião, Jéssica N. Silva, Carla de Oliveira Honse, Fabiano Borges Figueiredo, Denise T. da Silva, A. Kitada, Érica Guerino dos Reis, T. M. P. Schubach,
Sporotrichosis is caused by Sporothrix schenckii. The cat is the animal species most affected by this mycosis and plays an important role in the zoonotic transmission of this disease. The definitive diagnosis is made by isolation of the fungus in culture; however, cytopathological examination is frequently used in cats. Medical records from cats treated at Instituto de Pesquisa Clínica Evandro Chagas/Fiocruz, Rio de Janeiro, Brazil, between 2004 and 2006 were reviewed. Criteria for inclusion were ...
Tópico(s): Parasitic Infections and Diagnostics
2010 - SAGE Publishing | Journal of Feline Medicine and Surgery

Fabiano Borges Figueiredo, Maria F. Madeira, Lílian Dias Nascimento, Tuanne Rotti Abrantes, Eliame Mouta‐Confort, Sônia Regina Lambert Passos, Tânia Maria Pacheco Schubach,
The Brazilian Ministry of Health recommends the culling and euthanasia of dogs with a positive serological test for canine visceral leishmaniasis (CVL). In the Municipality of Rio de Janeiro, the technique used for the diagnosis of CVL is the indirect fluorescent antibody test (IFAT), using blood samples eluted on filter paper (eluate). A dog survey was conducted over a period of one year in the region of Carapiá, in order to evaluate the diagnosis of CVL in this region. All animals underwent clinical ...
Tópico(s): Trypanosoma species research and implications
2010 - UNIVERSIDADE DE SÃO PAULO | Revista do Instituto de Medicina Tropical de São Paulo

Fabiano Borges Figueiredo, Carlos José de Lima Barbosa Filho, Edvar Yuri Pacheco Schubach, Sandro Antônio Pereira, Lílian Dias Nascimento, Maria de Fátima Madeira,
O Brasil enfrenta uma expansão e urbanização da leishmaniose visceral americana com casos humanos e caninos em várias cidades de grande porte. O presente relato descreve um caso de leishmaniose visceral canina autóctone em uma área não endêmica no município de Rio de Janeiro.
Tópico(s): History of Medicine and Tropical Health
2010 - Brazilian Society of Tropical Medicine | Revista da Sociedade Brasileira de Medicina Tropical

Sandro Antônio Pereira, Sônia Regina Lambert Passos, J. N. Silva, Isabella Dib Ferreira Gremião, Fabiano Borges Figueiredo, José Liporage Teixeira, P. C. F. Monteiro, T. M. P. Schubach,
The effectiveness and safety of treatment with ketoconazole and itraconazole were compared in 773 sporotrichosis‐infected cats over a four‐year period (2002 to 2005). Five hundred and ninety‐eight cats received oral ketoconazole and 175 received oral itraconazole. Treatment was successful in 238 (30.8 per cent) cats, of which 171 (28.6 per cent) of 598 received 13.5 to 27.0 mg/kg/day ketoconazole and 67 (38.3 per cent) of 175 received 8.3 to 27.7 mg/kg/day itraconazole. Adverse effects were reported ...
Tópico(s): Infectious Diseases and Mycology
2010 - Wiley | Veterinary Record

Fabiano Borges Figueiredo, Isabella Dib Ferreira Gremião, Sandro Antônio Pereira, L.P. Fedulo, Rodrigo Caldas Menezes, D.A. Balthazar, T. M. P. Schubach, M.F. Madeira,
We report here the first known case of natural infection of a bush dog with Leishmania (Leishmania) chagasi in Brazil. The specimen was captured in the wild in the State of Mato Grosso and is currently being held in captivity at Fundação Jardim Zoológico, Rio de Janeiro, Brazil. The leishmaniasis was diagnosed by culture of promastigote forms in intact skin fragments and their characterization by isoenzyme electrophoresis. This report calls attention to the parasitological and etiological control ...
Tópico(s): Viral Infections and Vectors
2007 - Oxford University Press | Transactions of the Royal Society of Tropical Medicine and Hygiene

Luiz Rodrigo Paes Leme, T. M. P. Schubach, I.B Santos, Fabiano Borges Figueiredo, Sandro Antônio Pereira, Rodrigo S. Reis, Marcela Freire Vallim de Mello, Ana Maria Reis Ferreira, Leonardo Pereira Quintella, A Schubach,
Summary Twenty‐three cats with respiratory signs who had domiciliary contact with cats with sporotrichosis were studied. Sneezing was the predominant extracutaneous sign. Twelve cats had no skin lesions and 11 had ulcerated skin lesions. Mycological culture of material obtained from the nasal cavity, oral cavity, bronchoalveolar lavage (BAL) and skin lesions, when present, was performed for all cats. In the case of autopsy, lung fragments were cultured. Sporothrix schenckii was isolated from four ...
Tópico(s): Plant Pathogens and Fungal Diseases
2007 - Wiley | Mycoses

D.A. Silva, M.F. Madeira, Anna Carolina de Oliveira Resende Teixeira, Carina Margonari, Fabiano Borges Figueiredo,
In 2008, in the west zone of Rio de Janeiro municipality—Brazil, the leishmaniasis control program identified 155 dogs with titers ≥40 by Indirect ImmunoFluorescence (IIF) on blood collected onto filter paper. The objective of this study was to describe the laboratory test findings performed in dogs euthanized by the leishmaniasis program control of Rio de Janeiro municipality. Dogs were examined, subjected to euthanasia and collection of clinical specimens. Parasite isolation was obtained in 29 animals: ...
Tópico(s): Insect and Pesticide Research
2011 - Elsevier BV | Veterinary Parasitology

Luisa Helena Monteiro de Miranda, Leonardo Pereira Quintella, Rodrigo Caldas Menezes, Isabele Barbieri dos Santos, Raquel de Vasconcellos Carvalhães de Oliveira, Fabiano Borges Figueiredo, Leila M. Lopes‐Bezerra, T. M. P. Schubach,
The aim of this study was to apply immunohistochemistry (IHC) for the diagnosis of canine sporotrichosis and to compare this method with the Grocott's silver stain (GSS) and periodic acid Schiff (PAS) techniques. Eighty-seven dogs with sporotrichosis (group 1) and 35 with American tegumentary leishmaniosis (ATL) (group 2) were studied. The fungus was detected in group 1 by GSS, PAS and IHC. IHC was also applied to group 2 to evaluate the occurrence of cross-reactions. PAS, GSS and IHC detected yeast cells ...
Tópico(s): Nail Diseases and Treatments
2011 - Elsevier BV | The Veterinary Journal

Arleana do Bom Parto Ferreira de Almeida, Valéria Régia Franco Sousa, Eveline da Cruz Boa Sorte, Fabiano Borges Figueiredo, Daphine Ariadne Jesus de Paula, Maria Fernanda Aranega Pimentel, Valéria Dutra, Maria de Fátima Madeira,
In Brazil, although the domestic dog is a major target for the control actions for visceral leishmaniasis, knowledge gaps of the Leishmania species present in those animals still exist in many endemic areas. The objective of this study was the use of parasitological culture as a diagnosis tool and identification of species of Leishmania and other trypanosomatids in the canine population in the city of Cuiaba/Mato Grosso. Biological samples such as blood, intact skin fragments, cutaneous ulcers, ...
Tópico(s): Parasites and Host Interactions
2011 - Mary Ann Liebert, Inc. | Vector-Borne and Zoonotic Diseases

Maria de Fátima Madeira, Armando de Oliveira Schubach, T. M. P. Schubach, Sandro Antônio Pereira, Fabiano Borges Figueiredo, Cibele Baptista, Cristianni Antunes Leal, Cíntia X. Melo, Eliame Mouta Confort, M. C. A. Marzochi,
Tópico(s): Trypanosoma species research and implications
2006 - Elsevier BV | Veterinary Parasitology

Isabele Barbieri dos Santos, T. M. P. Schubach, Luiz Rodrigo Paes Leme, Thais Okamoto, Fabiano Borges Figueiredo, Sandro Antônio Pereira, Leonardo Pereira Quintella, Maria de Fátima Madeira, Flávia Gomes de Melo Coelho, Rosani dos Santos Reis, Armando de Oliveira Schubach,
Seventy-four dogs from the State of Rio de Janeiro with ulcerated cutaneous lesions were submitted to clinical, dermatological, parasitological, mycological, histopathological and cytopathological exams, a leishmanin skin test, an indirect immunofluorescence (IIF) test for leishmaniosis, and nonspecific laboratory tests such as blood count and serum biochemistry. Sporothrix schenckii was isolated from 41 dogs and Leishmania (Viannia) braziliensis was isolated from 33 animals. Most dogs with sporotrichosis ...
Tópico(s): T-cell and Retrovirus Studies
2006 - Elsevier BV | Veterinary Parasitology

T. M. P. Schubach, Armando O. Schubach, Thais Okamoto, Mônica B. L. Barros, Fabiano Borges Figueiredo, Tullia Cuzzi, Sandro Antônio Pereira, Isabele Barbieri dos Santos, Rodrigo de Almeida Paes, Luiz Rodrigo De Paes Leme, Bodo Wanke,
A sporotichosis epidemic involving forty-four dogs in the Metropolitan area of Rio de Janeiro is described. Solitary skin lesions were noted in 18 dogs (40.9%), 2–4 such lesions were observed in 17 animals (38.6%), and nine (20.5%) animals had five or more lesions. Twenty-five (56.8%) animals had single ulcerated skin lesions on the nose and nine (20.5%) showed nasal mucosal involvement (three of which also has a skin lesion). Respiratory symptoms were observed in 17 (38.6%) dogs and were found to be the ...
Tópico(s): Nail Diseases and Treatments
2006 - Oxford University Press | Medical Mycology

Isabel Cristina Fábregas Bonna, Fabiano Borges Figueiredo, Tatiana da Costa, Regiane Trigueiro Vicente, Charles André D. Santiago, José Leonardo Nicolau, Leandro Batista das Neves, Patrícia Riddell Millar, Leila Gatti Sobreiro, Maria Regina Reis Amendoeira,
A prevalencia da infeccao por Toxoplasma gondii em animais de regioes rurais e um importante indicador tanto do risco detransmissao para humanos como da presenca de oocistos do protozoario no ambiente. Com o objetivo de conhecer aprevalencia e analisar as principais variaveis de importância clinica-epidemiologica da infeccao por Toxoplasma gondii emsuinos e frangos no bairro de Santa Rita de Cassia, Barra Mansa, RJ, foi feito um estudo seccional soroepidemiologico. Dosproprietarios entrevistados, ...
Tópico(s): Rabies epidemiology and control
2006 - | Revista Brasileira de Ciência Veterinária

Rodrigo Caldas Menezes, Fabiano Borges Figueiredo, Annabel G. Wise, Maria F. Madeira, Raquel de Vasconcellos Carvalhães de Oliveira, T. M. P. Schubach, Matti Kiupel, Ingeborg M. Langohr,
ABSTRACT An accurate diagnosis of infection by Leishmania infantum in dogs is fundamental for the control of zoonotic visceral leishmaniasis (VL). Histopathology (HP) and immunohistochemistry (IHC) are frequently used for the histological diagnosis of L. infantum in dogs but have shown limited accuracy. To improve the sensitivity and specificity of the histological diagnosis of VL, we evaluated automated in situ hybridization (ISH) using a generic probe for Leishmania and a specific probe for L. infantum ...
Tópico(s): Dermatological diseases and infestations
2012 - American Society for Microbiology | Journal of Clinical Microbiology

Arleana do Bom Parto Ferreira de Almeida, Valéria Régia Franco Sousa, Felipe Augusto Constantino Seabra da Cruz, Magyda Arabia Araji Dahroug, Fabiano Borges Figueiredo, Maria de Fátima Madeira,
In Brazil, canine visceral leishmaniasis (CVL) is endemic and the number of cases in humans and dogs has increased in the Midwest region. A transversal study was carried out in endemic areas from Cuiabá, State of Mato Grosso, to assess data on seroprevalence and risk factors associated to canine infection. Four hundred and thirty (430) dogs were randomly evaluated through indirect fluorescence antibody test (IFAT) considering variables related to the animals, the environment and the knowledge by owners ...
Tópico(s): Leptospirosis research and findings
2012 - Colégio Brasileiro de Parasitologia Veterinaria | Revista Brasileira de Parasitologia Veterinária/Brazilian Journal of Veterinary Parasitology

Andreia Silva Alves, Eliame Mouta‐Confort, Fabiano Borges Figueiredo, Raquel de Vasconcellos Carvalhães de Oliveira, A Schubach, M.F. Madeira,
In order to evaluate if the presence of Trypanosoma caninum can lead to a confuse diagnosis of canine visceral leishmaniasis (CVL), we investigated the serological status of dogs infected by T. caninum and assessed the serological cross-reactivity with CVL. A set of 117 serum samples from dogs infected by T. caninum, Leishmania chagasi and not infected dogs (n = 39 in each group) was tested using commercial kits – indirect immunofluorescence (IFI-LVC), ELISA (EIE-LVC) and immunochromatographic test (DPP) – ...
Tópico(s): Eosinophilic Disorders and Syndromes
2012 - Elsevier BV | Research in Veterinary Science

Denise Amaro da Silva, Maria de Fátima Madeira, Tuanne Rotti Abrantes, Carlos José de Lima Barbosa Filho, Fabiano Borges Figueiredo,
An immunoenzymatic assay (ELISA), an indirect immunofluorescence antibody test (IFAT) with different antigens (ELISA-Leishmania chagasi, ELISA-L. major-like, IFAT-L. chagasi and IFAT-L. major-like), and an immunochromatographic test were assessed for the diagnosis of canine visceral leishmaniasis (CVL). Serum samples from 144 dogs from an endemic area for visceral leishmaniasis in the municipality of Rio de Janeiro were tested. The sensitivities of the serological tests were 93%, 100%, 73%, 60% and ...
Tópico(s): Virus-based gene therapy research
2012 - Elsevier BV | The Veterinary Journal