Produção Nacional
Revisado por pares
Fabiane Lacerda Francisco, Alessandro Morais Saviano, Terezinha de Jesus Andreoli Pinto, Felipe Rebello Lourenço,
Microbiological assays have been used to evaluate antimicrobial activity since the discovery of the first antibiotics. Despite their limitations, microbiological assays are widely employed to determine antibiotic potency of pharmaceutical dosage forms, since they provide a measure of biological activity. The aim of this work is to develop, optimize and validate a rapid colorimetric microplate bioassay for the potency of neomycin in pharmaceutical drug products. Factorial and response surface methodologies ...
Tópico(s): Antibiotics Pharmacokinetics and Efficacy
2014 - Elsevier BV | Journal of Microbiological Methods
Produção Nacional
Revisado por pares
Fabiane Lacerda Francisco, Alessandro Morais Saviano, Terezinha de Jesus Andreoli Pinto, Felipe Rebello Lourenço,
Background: Microbiological assay is the gold-standard method for analyzing antibiotics, since it ensures the success of antimicrobial therapy. However, this method may be time-consuming and it remains almost unaltered since it was first developed. Here, we present the design space of a rapid microplate bioassay for bacitracin. Methods: Triphenyltetrazolium chloride was used in the bioassay to provide faster response. Factorial design and response surface methodologies were used to optimize microorganism, ...
Tópico(s): bioluminescence and chemiluminescence research
2016 - Bentham Science Publishers | Current Analytical Chemistry
Produção Nacional
Revisado por pares
Mary Toshie Kayano, Wilmar L. Cerón, Rita V. Andreoli, Rodrigo Paiva Souza, Álvaro Ávila-Díaz, Cristian Felipe Zuluaga, Leila M. V. Carvalho,
Previous studies have shown that the Atlantic Multidecadal Oscillation (AMO) and Pacific Decadal Oscillation (PDO) have combined effects on the precipitation (PRP) variability over South America. The combined impacts have been assessed considering four mean states as the averages of the variable anomalies during sub-periods overlapping time intervals of the PDO and AMO phases. Since these sub-periods include years under El Niño-Southern Oscillation (ENSO) extremes, the extent to which these years’ occurrence ...
Tópico(s): Plant Water Relations and Carbon Dynamics
2022 - Multidisciplinary Digital Publishing Institute | Atmosphere
Produção Nacional
Revisado por pares
Daniela Dal Molim Ghisleni, Rogério Takao Okamoto, Cleide Maria de Oliveira Amaral, Felipe Rebello Lourenço, Terezinha de Jesus Andreoli Pinto,
Caspofungin is an echinocandin antifungal used in the treatment of invasive fungal infections. Several methods have been reported for the quantitative analysis of echinocandins; however, there is no microbiological assay for determination of caspofungin potency in the presence of its degradation products. This study aimed to develop and validate a microbiological method for quantitative analysis of caspofungin in lyophilized powder, evaluate the stability, and determinate the degradation kinetics ...
Tópico(s): Antibiotics Pharmacokinetics and Efficacy
2014 - Oxford University Press | Journal of AOAC International
Produção Nacional
Revisado por pares
Felipe Rebello Lourenço, Terezinha de Jesus Andreoli Pinto,
The aim of this study was to determine the optimal experimental conditions to develop a methodology for microbiological assay of apramycin employing microplate and kinetic reading mode, and to validate the developed method, through evaluation of parameters of selectivity, linearity, linear range, limits of detection and quantification, accuracy and precision. The turbidimetric assay principle is simple: the test solution is added to a suspension of test microorganism in culture media, the mixture ...
Tópico(s): Biosensors and Analytical Detection
2011 - UNIVERSIDADE DE SÃO PAULO | Brazilian Journal of Pharmaceutical Sciences
Felipe Rebello Lourenço, Terezinha de Jesus Andreoli Pinto,
Gentamicin is a broad-spectrum antibiotic complex produced by actinomycetes belonging to Micromonospora genus and classified among aminoglycoside antibiotics, used in the treatment of serious infections derived from Gram-negative microorganisms. Alterations of their antimicrobial activity not shown in chemical assays can be evaluated through microbiological assays. The aim of this work was to compare 5 x 1, 2 x 2 and 3 x 1 experimental designs, evaluating validation parameters of specificity, linearity, ...
Tópico(s): Antifungal resistance and susceptibility
2009 - UNIVERSIDADE DE SÃO PAULO | Brazilian Journal of Pharmaceutical Sciences
Produção Nacional
Revisado por pares
Débora Cristina de Oliveira, Leandro Augusto Calixto, Isa Martins Fukuda, Alessandro Morais Saviano, Adir José Moreira, Yoshio Kawano, Ronaldo Domingues Mansano, Terezinha de Jesus Andreoli Pinto, Felipe Rebello Lourenço,
Tópico(s): Polymer Science and PVC
2018 - Springer Science+Business Media | Journal of Pharmaceutical Innovation
Felipe Rebello Lourenço, Terezinha de Jesus Andreoli Pinto,
SUMMARY. Pharmaceutical equivalence is an important step towards the confirmation of similarity and interchangeability among pharmaceutical products, particularly regarding those that will not be tested for bioequivalence. The aim of this paper is to compare traditional difference testing to two one-side equivalence tests in the assessment of pharmaceutical equivalence, by means of equivalence studies between similar, generic and reference products of acyclovir cream, atropine sulfate injection, ...
Tópico(s): Pharmaceutical Economics and Policy
2012 - College of Pharmacists of Buenos Aires Province | Latin American Journal of Pharmacy
Produção Nacional
Revisado por pares
Elisabete de Almeida Barbosa Antunes, Felipe Rebello Lourenço, Terezinha de Jesus Andreoli Pinto,
A high-performance liquid chromatographic method employing pre-column derivatization with o-phthalaldehyde (OPA) and 2-mercaptoacetic acid was developed for the determination of apramycin, an aminoglycoside antibiotic used in veterinary medicine, in the oral soluble powder form. The chromatographic separation was done by ion-pair HPLC using a C18 reversed-phase column, Synergy Hydro (150 mm x 4.6 mm x 4 µm) and mobile phase composed of 0.005 mol/L sodium octanosulfonate in a mixture of acetonitrile: ...
Tópico(s): Synthesis and Biological Evaluation
2011 - UNIVERSIDADE DE SÃO PAULO | Brazilian Journal of Pharmaceutical Sciences
Produção Nacional
Revisado por pares
Mariana Ferreira Leal, Leonardo Caires Santos, Adrielle Martins de Oliveira, Paulo Santoro Belangero, Eduardo Antônio de Figueiredo, Carina Cohen, Felipe de Seixas Alves, Wânia Hiromi Yanaguizawa, Carlos Vicente Andreoli, Alberto de Castro Pochini, Benno Ejnisman, Marı́lia de Arruda Cardoso Smith, Maria Teresa de Seixas Alves, Moisés Cohen,
Rotator cuff tear is a common orthopedic condition. Metalloproteinases (MMP) and their inhibitors (TIMP) seem to play a role in the development of joint injuries and in the failure of tissue healing. However, the mechanisms of regulation of gene expression in tendons are still unknown. Epigenetic mechanisms, such as DNA methylation and microRNAs regulation, are involved in the dynamic control of gene expression. Here, the mRNA expression and DNA methylation status of MMPs (MMP1, MMP2, MMP3, MMP9, MMP13, ...
Tópico(s): Pulmonary Hypertension Research and Treatments
2017 - Public Library of Science | PLoS ONE
Felipe Rebello Lourenço, Elisabete A. Barbosa, Terezinha de Jesus Andreoli Pinto,
Tópico(s): Synthesis and Biological Evaluation
2011 - College of Pharmacists of Buenos Aires Province | Latin American Journal of Pharmacy
Produção Nacional
Revisado por pares
Felipe Rebello Lourenço, Telma Mary Kaneko, Terezinha de Jesus Andreoli Pinto,
Abstract Methodology validation and measurement uncertainty estimation are fundamental to obtain reliable results. The microbiological methods are widely used to determine antibiotic assay, as they permit evaluation of the analyzed antibiotic activity. A microbiological assay of vancomycin was performed with adoption of experimental design 5 1 (interpolation in 5-point standard curve assay) with final concentrations from 6.4 to 15.6 g/mL (standards) and 10.0 g/mL (sample). Bacillus subtitlis (ATCC 6633) ...
Tópico(s): Antibiotics Pharmacokinetics and Efficacy
2007 - Oxford University Press | Journal of AOAC International
Produção Nacional
Revisado por pares
Túlia de Souza Botelho, Felipe Rebello Lourenço, Terezinha de Jesus Andreoli Pinto,
The aim of this work was to develop a microbiological assay of vancomycin employing microplates and kinetic reading mode, and to validate the developed method by assessing of specificity and selectivity, linearity, linear range, accuracy and precision parameters. Established conditions comprised a vancomycin standard-curve in concentrations from 0.6 to 1.0 μg mL-1, and soybean-casein broth inoculated with 5% of Bacillus subtilis (ATCC 6633) suspension. Satisfactory results were obtained after a 5 ...
Tópico(s): Probiotics and Fermented Foods
2013 - Bentham Science Publishers | Current Pharmaceutical Analysis
Produção Nacional
Revisado por pares
Felipe Rebello Lourenço, Túlia de Souza Botelho, Terezinha de Jesus Andreoli Pinto,
The limulus amebocyte lysate (LAL) test is the simplest and most widely used procedure for detection of endotoxin in parenteral drugs. The LAL test demands optimal pH, ionic strength, temperature, and time of incubation. Slight changes in these parameters may increase the frequency of false-positive responses and the estimated uncertainty of the LAL test. The aim of this paper is to evaluate how changes in the pH, temperature, and time of incubation affect the occurrence of false-positive responses ...
Tópico(s): Renal function and acid-base balance
2012 - Parenteral Drug Association | PDA Journal of Pharmaceutical Science and Technology
Daniela Dal Molim Ghisleni, Rogério Takao Okamoto, Felipe Rebello Lourenço, Terezinha de Jesus Andreoli Pinto,
This work aims to develop and validate a UPLC stability indicating method for quantitative analysis of Caspofungin, grounded in statistical analysis able to estimate measurement uncertainty, and determine drug degradation kinetics. The validation was performed as recommended in ICH guidelines. The chromatographic method was conducted applying the following conditions: 220 nm wavelength, 10 μL injection volume, 25 °C oven temperature, 0.6 mL/minute flow, and gradient elution involving mobile phases ...
Tópico(s): Phytochemistry and Biological Activities
2014 - Bentham Science Publishers | Current Pharmaceutical Analysis
Produção Nacional
Revisado por pares
Felipe Rebello Lourenço, Fabiane Lacerda Francisco, Márcia Regina Spuri Ferreira, Terezinha De Jesus Andreoli, Raimar Löbenberg, Nádia Araci Bou‐Chacra,
The use of preservatives must be optimized in order to ensure the efficacy of an antimicrobial system as well as the product safety. Despite the wide variety of preservatives, the synergistic or antagonistic effects of their combinations are not well established and it is still an issue in the development of pharmaceutical and cosmetic products. The purpose of this paper was to establish a space design using a simplex-centroid approach to achieve the lowest effective concentration of 3 preservatives ( ...
Tópico(s): Antimicrobial Peptides and Activities
2015 - Canadian Society for Pharmaceutical Sciences | Journal of Pharmacy & Pharmaceutical Sciences
Produção Nacional
Revisado por pares
Túlia de Souza Botelho-Almeida, Felipe Rebello Lourenço, Irene Satiko Kikuchi, Rajendra Awasthi, Kamal Dua, Terezinha de Jesus Andreoli Pinto,
Tópico(s): Listeria monocytogenes in Food Safety
2018 - Springer Science+Business Media | Journal of Pharmaceutical Innovation
Produção Nacional
Revisado por pares
Carine Goncalves Tavora, Paulo Roberto de Aguiar, Bruno Albuquerque de Castro, Felipe Aparecido Alexandre, André Luiz Andreoli, Eduardo Carlos Bianchi,
Tópico(s): Mineral Processing and Grinding
2021 - Springer Science+Business Media | The International Journal of Advanced Manufacturing Technology
Felipe Rebello Loureno, Telma Mary Kaneko, Terezinha de Jesus Andreoli Pinto,
The agar diffusion method, widely used in antibiotic dosage, relates the diameter of the inhibition zone to the dose of the substance assayed. An experimental plan is proposed that may provide better results and an indication of the assay validity. The symmetric or balanced assays (2 x 2) as well as those with interpolation in standard curve (5 x 1) are the main designs used in the dosage of antibiotics. This study proposes an alternative experimental design for erythromycin microbiological assay ...
Tópico(s): Protein purification and stability
2007 - Oxford University Press | Journal of AOAC International
Produção Nacional
Felipe Rebello Lourenço, Telma Mary Kaneko, Terezinha de Jesus Andreoli Pinto,
Desde a publicação da ISO 17025:1999, o interesse em métodos para estimativa da incerteza em ensaios qualitativos, do tipo "passa/não passa", têm ganho grande importância. Uma forma de estimar e informar a incerteza deste tipo de ensaio é o uso das probabilidades de respostas-falsas, particularmente falsos-positivos e falsos-negativos, determinados a partir do teorema de Bayes. O objetivo deste artigo é estabelecer um método para a estimativa de incerteza em ensaios de detecção de endotoxina bacteriana ...
Tópico(s): Multi-Criteria Decision Making
2005 - UNIVERSIDADE DE SÃO PAULO | Revista Brasileira de Ciências Farmacêuticas
Felipe Rebello Lourenço, Marcus Augusto Lyrio Traple, Rogério Takao Okamoto, Terezinha de Jesus Andreoli Pinto,
The aim of this work is to develop and validate a microbiological assay for ceftriaxone with a view to the employment of this method to assess photo-stability in commercial products. The experimental conditions included: (1) ceftriaxone standard solution in concentrations of 2.6 μg/mL, 3.2 μg/mL, 4.0 μg/mL, 5.0 μg/mL and 6.3 μg/mL, using phosphate buffer pH 6.0 as diluent, (2) Petri dishes prepared by adding 21-mL of antibiotic medium as base layer and 4-mL of the same medium, inoculated with Bacillus ...
Tópico(s): Pharmaceutical Quality and Counterfeiting
2013 - Bentham Science Publishers | Current Pharmaceutical Analysis
Produção Nacional
Revisado por pares
Martin Antonio Aulestia Viera, Paulo Roberto de Aguiar, Pedro Oliveira, Felipe Aparecido Alexandre, Wenderson Nascimento Lopes, Eduardo Carlos Bianchi, Rosemar Batista da Silva, Doriana M. D’Addona, André Luiz Andreoli,
Innovative monitoring systems based on sensor signals have emerged in recent years in view of their potential for diagnosing machining process conditions. In this context, preliminary applications of fast-response and low-cost piezoelectric diaphragms (PZT) have recently emerged in the grinding monitoring field. However, there is a lack of application regarding the grinding of ceramic materials. Thus, this work presents an analysis of the feasibility of using the acoustic emission signals obtained ...
Tópico(s): Advanced Surface Polishing Techniques
2019 - Multidisciplinary Digital Publishing Institute | Sensors
Produção Nacional
Revisado por pares
Paulo Santoro Belangero, Eduardo Antônio de Figueiredo, Carina Cohen, Felipe de Seixas Alves, Wânia Hiromi Yanaguizawa, Marı́lia de Arruda Cardoso Smith, Carlos Vicente Andreoli, Alberto de Castro Pochini, Maria Teresa de Seixas Alves, Benno Ejnisman, Moisés Cohen, Mariana Ferreira Leal,
ABSTRACT Lack of synthesis of extracellular matrix compounds may contribute to degeneration of the tendons. Thus, we aimed to evaluate the expression of extracellular matrix and TGFB family members in ruptured and non‐ruptured tendons of the rotator cuff, as well as the effect of clinical factors on gene expression in tendon samples, and the relationship between histological findings and altered gene expression. Injured and non‐injured supraspinatus tendon samples and subscapular non‐injured tendon ...
Tópico(s): Shoulder and Clavicle Injuries
2018 - Wiley | Journal of Orthopaedic Research®
Produção Nacional
Revisado por pares
Rodrigo L. Moura, Maria L. Abieri, Guilherme M. Castro, Lélis Antonio Carlos-Júnior, Pamela M. Chiroque-Solano, Nicole Fernandes, Carolina D. Teixeira, Felipe V. Ribeiro, Paulo S. Salomon, Matheus Oliveira Freitas, Juliana T. Gonçalves, Leonardo M. Neves, Carlos Werner Hackradt, Fabiana Cézar Félix‐Hackradt, Fernanda Andreoli Rolim, Fábio S. Motta, Otto Bismarck Fazzano Gadig, Guilherme H. Pereira‐Filho, Alex Cardoso Bastos,
Abstract Understanding habitat-level variation in community structure provides an informed basis for natural resources’ management. Reef fishes are a major component of tropical marine biodiversity, but their abundance and distribution are poorly assessed beyond conventional SCUBA diving depths. Based on a baited-video survey of fish assemblages in Southwestern Atlantic’s most biodiverse region we show that species composition responded mainly to the two major hard-bottom megahabitats (reefs and ...
Tópico(s): Marine and coastal plant biology
2021 - Nature Portfolio | Scientific Reports
Catherine Simoes de Abreu, Felipe Rebello Lourenço, Terezinha de Jesus Andreoli Pinto,
The antimicrobial activity of five sanitizing agents employed in clean areas designated for the pharmaceutical manufacturing of sterile products was tested against nine microorganisms, including four microorganisms from the clean area microbiota. The method consisted of challenging 5 mL of each sanitizing agent - 70% isopropyl alcohol, 0.4% LPH®, 1.16% hydrogen peroxide, 4% hydrogen peroxide, 1% Bioper® and 5% phenol - with 0.1mL each of concentrated suspensions (105 – 106 CFU/mL) of Staphylococcus aureus, ...
Tópico(s): Listeria monocytogenes in Food Safety
2011 - UNIVERSIDADE EST.PAULISTA JÚLIO DE MESQUITA FILHO | Revista de Ciências Farmacêutica Básica e Aplicadas - RCFBA
Produção Nacional
Revisado por pares
Márcia Regina Spuri Ferreira, Felipe Rebello Lourenço, M T Ohara, Nádia Araci Bou‐Chacra, Terezinha de Jesus Andreoli Pinto,
Freeze-dried bacteria and fungi were used as inoculum in 28 days' PET. An electrical method was used in replacement of the conventional plate count method. The use of freeze-dried microorganisms in association with the electrical method can minimize the workload and the variability involved in PET for cosmetic powders.
Tópico(s): 3D Printing in Biomedical Research
2014 - Elsevier BV | Journal of Microbiological Methods
Produção Nacional
Revisado por pares
Luiz Augusto Borges Gomes, Ronaldo Alves da Cunha, Alexandre Días Lópes, Fábio Andrelino de Souza, Felipe Cruvinel Costa, Carlos Vicente Andreoli,
Lower limb injuries generate a significant health burden in basketball. Landing technique and ankle-dorsiflexion range of motion have been suggested as risk factors for lower limb injuries among youth athletes, but studies conducted specifically with basketball athletes are lacking.
Tópico(s): Cardiovascular Effects of Exercise
2023 - | International Journal of Sports Physical Therapy
Produção Nacional
Revisado por pares
Felipe Rebello Loureno, Irene Satiko Kikuchi, Terezinha de Jesus Andreoli Pinto,
Silicone is a polymer widely used for medical purposes, including breast implants. The aim of this work is to verify the occurrence of silicone gel diffusion through the lining membrane (bleed) of gel breast implants with smooth, textured, and polyurethane foam coated surfaces, sterilized by dry heat and ethylene oxide. The results enable us to conclude that there is significant difference for the bleed results among gel breast implants of smooth, textured, and polyurethane foam coated surfaces in ...
Tópico(s): Additive Manufacturing and 3D Printing Technologies
2011 - Bentham Science Publishers | The Open Biomaterials Journal
Produção Nacional
Alberto de Castro Pochini, Mário Ferretti, Eduardo Felipe Kin Ito Kawakami, Artur da Rocha Corrêa Fernandes, André Fukunishi Yamada, Gabriela Clemente de Oliveira, Moisés Cohen, Carlos Vicente Andreoli, Benno Ejnisman,
To evaluate tendinopathy of the pectoralis major muscle in weightlifting athletes using ultrasound and elastography.This study included 20 patients, 10 with rupture of the pectoralis major muscle and 10 control patients. We evaluated pectoralis major muscle contralateral tendon with ultrasonographic and elastography examinations. The ultrasonographic examinations were performed using a high-resolution B mode ultrasound device. The elastography evaluation was classified into three patterns: (A), if ...
Tópico(s): Sports injuries and prevention
2015 - Instituto Israelita de Ensino e Pesquisa Albert Einstein | Einstein (São Paulo)
Felipe Rebello Lourenço, Daniela Dal Molim Ghisleni, Rosa Noriko Yamamoto, Terezinha de Jesus Andreoli Pinto,
The aim of this work is to present the two one-sided test (TOST) as an alternative approach to compare dissolution profiles of extended-release dosage forms. The dissolution profiles of oxycodone extended-release tablets containing 10 mg, 20 mg and 40 mg (reference and generic) were evaluated according to the requirements described in United States Pharmacopeia. These dissolution profiles were compared using the conventional similarity factor (f2) and the proposed TOST as an equivalence test. TOST ...
Tópico(s): Crystallization and Solubility Studies
2013 - UNIVERSIDADE DE SÃO PAULO | Brazilian Journal of Pharmaceutical Sciences