Sara Gonçalves, Jorge Padrão, Inês Rodrigues, João Pedro Silva, Vítor Sencadas, S. Lanceros‐Méndez, Henrique Girão, Fernando Dourado, L. R. Rodrigues,
The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 μm of average thickness) and heat-dried BC substrates were surface-modified via acetylation and polysaccharide adsorption, using chitosan and carboxymethyl cellulose. All substrates were characterized according to their surface chemistry, wettability, energy, topography, and also regarding their permeability, dimensional stability, mechanical properties, and endotoxin ...
Tópico(s): Corneal Surgery and Treatments
2015 - American Chemical Society | Biomacromolecules

Rodolfo de Melo Nunes, Morgana Ramos Martins, Francisco Saraiva da Silva, Ana Caroline Rocha de Melo Leite, Virgínia Cláudia Carneiro Girão‐Carmona, Fernando Q. Cunha, Aryana Lushese Lima Feitosa Marinho, Ana Carolina Matias Dinelly Pinto, Francisco Airton Castro Rocha,
We investigated the anti-inflammatory activity of strontium ranelate (SR) in arthritis models.Rats received 1 mg zymosan (Zy) or saline intra-articularly. Other groups were subjected to anterior cruciate ligament transection in the right knee, as an osteoarthritis (OA) model, or a sham procedure. Joint pain was assessed using the articular incapacitation and paw-pressure tests. Cell influx and cytokines were measured in joint exudates.Groups received either SR (30-300 mg/kg per os) or saline.SR dose- ...
Tópico(s): Anesthesia and Pain Management
2015 - Springer Science+Business Media | Inflammation Research
Luís Costa-Matos, Paulo Ricardo Batista, Nuno Monteiro, Pedro Henriques, Fernando Girão, Armando Carvalho,
ContextAlcoholic liver disease (ALD) is generally associated with iron overload, which may contribute to its pathogenesis, through increased oxidative stress and cellular damage. There are conflicting reports in literature about hemochromatosis (HFE) gene mutations and the severity of liver disease in alcoholic patients.ObjectivesTo compare the prevalence of mutations in the hemochromatosis (HFE) gene between patients with ALD and healthy controls; to assess the relation of HFE mutations with liver ...
Tópico(s): Hemoglobinopathies and Related Disorders
2013 - Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia (IBEPEGE) | Arquivos de Gastroenterologia
Luís Costa Matos, Paulo Ricardo Batista, Nuno Monteiro, João Ribeiro, Maria Augusta Cipriano, Pedro Henriques, Fernando Girão, Armando Carvalho,
Background: The relation between alcoholic liver disease (ALD) and iron overload is well known. Liver biopsy is the gold standard for assessing iron stores. MRI is also validated for liver iron concentration (LIC) assessment. We aimed to assess the effect of active drinking in liver iron stores and the practicability of measuring LIC by MRI in ALD patients. Materials and methods: We measured LIC by MRI in 58 ALD patients. We divided patients into two groups – with and without active alcoholism – and we ...
Tópico(s): Iron Metabolism and Disorders
2013 - Taylor & Francis | Scandinavian Journal of Gastroenterology
Luís Costa-Matos, Paulo Ricardo Batista, Nuno Monteiro, Maria José Simões, Conceição Egas, Jorge Pereira, Helena Pinho, Natália Gomes dos Santos, João Ribeiro, Maria Augusta Cipriano, Pedro Henriques, Fernando Girão, Alfredo José Rodrigues, Armando Carvalho,
Hepcidin plays a crucial role in iron metabolism, preventing its absorption at the basolateral enterocyte membrane. Hepcidin regulation is complex and regulated at the transcriptional level. The relation between iron overload and alcoholic liver disease is well known, but its mechanism is not clear. We present an observational, case-control study, aimed at evaluating the effects of alcohol on the expression of hepcidin in human participants. We intended to assess whether iron overload related to ...
Tópico(s): Hemoglobinopathies and Related Disorders
2012 - Lippincott Williams & Wilkins | European Journal of Gastroenterology & Hepatology

Ícaro Girão Evangelista, Fernando Bruno Pontes Tabosa, Ariel Valente Bezerra, Eliziário Vitoriano de Araújo Neto,
Introduction: Orofacial paresthesia is due to trauma to the neural structure of a particular nerve. In dentistry alterations caused by nerve damage in most cases are presented with transient symptomatology. However, it has been agreed by several authors that persistent inferior alveolar sensory aberrations for more than 6 months leave some degree of disability or are considered permanent. The objective of the present study is to report the clinical case of a young patient submitted to low-level ...
Tópico(s): Dental Radiography and Imaging
2019 - | Journal of lasers in medical sciences

Aline P Carvalho, Mirna Marques Bezerra, Virgínia Cláudia Carneiro Girão‐Carmona, Fernando Q. Cunha, Francisco Airton Castro Rocha,
SUMMARY The antinociceptive effect of risedronate in experimental pain models in rats and mice was investigated in the present study. Rats received zymosan intra‐articularly (i.art.) into the right knee joint and the nociceptive response was assessed using the articular incapacitation test. Joint washouts were used for determining cell influx, tumour necrosis factor (TNF)‐α and leukotriene (LT) B 4 levels. Mice received either zymosan (1 mg) or acetic acid (0.6%) i.p. and the nociceptive response was ...
Tópico(s): Neuropeptides and Animal Physiology
2006 - Wiley | Clinical and Experimental Pharmacology and Physiology
Ricardo Filipe da Silva Antunes, Cláudia Valéria Seullner Brandão, Gonçalo Carvalho, Cristina Girão, Fernando A. Arosa,
Tópico(s): Erythrocyte Function and Pathophysiology
2009 - Springer Nature | Cellular and Molecular Life Sciences
Eurico Oliveira, Paula Manuel, Alexandre João, Fernando Girão,
A 79-year-old man presented with an 8 h history of shortness of breath and oedematous legs. He had a history of hypertension, atrial fibrillation, and chronic obstructive pulmonary lung disease. He was on long term ipratropium bromide, irbesartan, amlodipine, amiodarone, and furosemide. He had normal vital signs and the only relevant findings in physical examination were bilateral oedema of both legs and rales in the lower half of both lungs. Radiograph of the chest showed an opaque elliptic image ( ...
Tópico(s): Vascular Malformations and Hemangiomas
2012 - Elsevier BV | The Lancet
Sara Gonçalves, Inês Rodrigues, Jorge Padrão, João Pedro Silva, Vítor Sencadas, S. Lanceros‐Méndez, Henrique Girão, Miguel Gama, Fernando Dourado, L. R. Rodrigues,
This work evaluated the effect of acetylated bacterial cellulose (ABC) substrates coated with urinary bladder matrix (UBM) on the behavior of retinal pigment epithelium (RPE), as assessed by cell adhesion, proliferation and development of cell polarity exhibiting transepithelial resistance and polygonal shaped-cells with microvilli. Acetylation of bacterial cellulose (BC) generated a moderate hydrophobic surface (around 65°) while the adsorption of UBM onto these acetylated substrates did not affect ...
Tópico(s): Corneal Surgery and Treatments
2015 - Elsevier BV | Colloids and Surfaces B Biointerfaces

Lucas Villas Bôas Hoelz, André A. S. T. Ribeiro, Rafael C. Bernardi, Bruno A. C. Horta, Magaly Girão Albuquerque, Fernando Moreira da Silva, Pedro G. Pascutti, Ricardo Bicca de Alencastro,
Abstract The human β1-adrenoceptor (β1AR) is a G-protein-coupled receptor (GPCR) involved in sympathetic system regulation through agonist-induced activation. The conserved CWXP-motif in helix 6 (rotamer toggle switch) is one of the most important activation switches in Class A GPCRs. In order to investigate how the agonist binding disturbs this switch, we carried out molecular dynamics simulations of a hβ1AR model in the apo and R-noradrenaline-bound forms. The results show that the agonist binding ...
Tópico(s): Neuropeptides and Animal Physiology
2011 - Taylor & Francis | Molecular Simulation

Lucas Villas Bôas Hoelz, Rafael C. Bernardi, Bruno A. C. Horta, Jocley Queiroz Araújo, Magaly Girão Albuquerque, Fernando Moreira da Silva, Pedro G. Pascutti, Ricardo Bicca de Alencastro,
The human β1-adrenoceptor (hβ1AR) is a transmembrane (TM) protein responsible for the signal transduction pathway via agonist interaction. Despite its importance, hβ1AR activation mechanism is still unclear. The most studied and widely accepted mechanism is the disruption of a salt bridge between TM3 arginine and TM6 glutamic acid, called ionic lock. In this work, we constructed a functional hβ1AR-model equilibrated in a membrane environment to study the influence of agonist binding on the dynamical ...
Tópico(s): Mass Spectrometry Techniques and Applications
2011 - Taylor & Francis | Molecular Simulation
Sara Gonçalves, Jorge Padrão, Inês Rodrigues, João Pedro Silva, Vítor Sencadas, S. Lanceros‐Méndez, Henrique Girão, Fernando Dourado, L. R. Rodrigues,
... Pedro Silva†, Vítor Sencadas∥, Senentxu Lanceros-Mendez∥, Henrique Girão‡, Fernando Dourado†, and Lígia R. Rodrigues*†View Author Information† † ...
Tópico(s): Enzyme Production and Characterization
2015 - American Chemical Society | Biomacromolecules

Francisco Airton Castro Rocha, F.S. Silva, ACRM Leite, AKRM Leite, Virgínia Cláudia Carneiro Girão‐Carmona, R Gil de Castro, Fernando Q. Cunha,
We investigated the effect of the phosphodiesterase-5 inhibitor, tadalafil, on the acute hypernociception in rat models of arthritis.Rats were treated with either an intra-articular injection of zymosan (1 mg) or surgical transection of the anterior cruciate ligament (as an osteoarthritis model). Controls received saline intra-articular or sham operation respectively. Joint pain was evaluated using the articular incapacitation test measured over 6 h following zymosan or between 4 and 7 days after ...
Tópico(s): Mast cells and histamine
2011 - Wiley | British Journal of Pharmacology
António Completo, A. Nascimento, André F. Girão, Fernando Fonseca,
Pyrocarbon proximal interphalangeal joint arthroplasty provided patients with excellent pain relief and joint motion, however, overall implant complications have been very variable, with some good outcomes at short-medium-term follow-up and some bad outcomes at longer-term follow-up. Implant loosening with migration, dislocation and implant fracture were the main reported clinical complications. The aim of the present work was to test the hypothesis that the magnitude proximal interphalangeal joint ...
Tópico(s): Orthopedic Infections and Treatments
2018 - Elsevier BV | Clinical Biomechanics

Jéssica Suellen Sena, Régio José Santiago Girão, Sionara Melo Figueiredo de Carvalho, Rosielly Melo Tavares, Fernando Luiz Affonso Fonseca, Patrícia Barros Aquino Silva, Maria Clara Fortes Portela Barbosa,
SUMMARY Objective: To analyze the epidemiological profile, risk factors in the workplace environment and prevention methods for professionals at risk of skin cancer. Method: A systematic review of articles on occupational skin cancer, published in the Lilacs, Scielo, Medline and Cochrane Library from January 1st, 2008, to December 31st, 2013, was performed. The search included the following terms: “neoplasias cutâneas” (DeCS), “exposição ocupacional” (DeCS), “epidemiologia” (DeCS) as well as the keyword “ ...
Tópico(s): Nonmelanoma Skin Cancer Studies
2016 - Brazilian Medical Association | Revista da Associação Médica Brasileira

Saulo Cabral Bourguignon, Danielle F. B. Cavalcanti, Alessandra Mendonça Teles de Souza, Helena Carla Castro, Carlos Rangel Rodrigues, Magaly Girão Albuquerque, Dilvani Oliveira Santos, Gabriel Gomes da Silva, Fernando de Carvalho da Silva, Vı́tor F. Ferreira, Rosa T. Pinho, Carlos Roberto Alves,
In this study we compared the effects of naphthoquinones (α-lapachone, β-lapachone, nor-β-lapachone and Epoxy-α-lap) on growth of Trypanosoma cruzi epimastigotes forms, and on viability of VERO cells. In addition we also experimentally analyzed the most active compounds inhibitory profile against T. cruzi serine- and cysteine-proteinases activity and theoretically evaluated them against cruzain, the major T. cruzi cysteine proteinase by using a molecular docking approach. Our results confirmed β- ...
Tópico(s): Trypanosoma species research and implications
2010 - Elsevier BV | Experimental Parasitology

Francisco Airton Castro Rocha, Mauro Martins Teixeira, José Rocha, Virgínia Cláudia Carneiro Girão‐Carmona, Mirna Marques Bezerra, Ronaldo de Albuquerque Ribeiro, Fernando Q. Cunha,
We investigated whether leukotrienes mediate cell influx and articular incapacitation in zymosan-induced arthritis. Rats received 1 mg zymosan intra-articularly (i.a.). The hyperalgesia was measured using the rat articular incapacitation test. Cell influx, leukotriene B4 and prostaglandin E2 levels were assessed in the joint exudate, at 6 h. Groups received either the leukotriene B4 synthesis inhibitor MK 886 (3-[1-(4-chlorobenzyl)-3-t-butyl-thio-5-isopropylindol-2-yl)]-2,2-dimethylpropanoic acid ...
Tópico(s): Asthma and respiratory diseases
2004 - Elsevier BV | European Journal of Pharmacology
Pedro M. Ramos, Fernando M. Janeiro, Pedro Silva Girão,
The paper discusses the evaluation of the uncertainty of a multivariate quantity using the Law of Propagation of Uncertainty defined in the Guide to the Expression of Uncertainty in Measurement (GUM) and a Monte Carlo method according to the GUM's Supplement 2. The quantity analysed is the electrical impedance, which is not a scalar but a complex quantity. The used measuring method allows the evaluation of the impedance and of its uncertainty in different ways and the corresponding results are presented, ...
Tópico(s): Advanced Sensor Technologies Research
2015 - Elsevier BV | Measurement

Marluci Palazzolli Silva, Fabrício Luiz Tulini, Fernando Eustáquio de Matos-Jr, Mariana Girão de Oliveira, Marcelo Thomazini, Carmen Sílvia Fávaro-Trindade,
Probiotics were enumerated in each step of the encapsulation process and the resistance of encapsulated microorganisms was verified under stress (temperature, pH, sodium chloride and sucrose) and simulated gastrointestinal conditions (SGC). Lipid particles were effective in protecting the probiotics subjected to environmental stress conditions (ESC) and SGC, when compared to free cells. After 30 days of storage at 25 °C, the viability of probiotics encapsulated by spray chilling was about 7.2 log cfu/ ...
Tópico(s): Proteins in Food Systems
2018 - Elsevier BV | Food Hydrocolloids
Andrea Balreira, M. Fátima Macedo, Cristina Girão, Lorena G. Rodrigues, João Paulo Oliveira, Clara Sá-Miranda, Fernando A. Arosa,
Fabry disease (FD), an X-linked sphingolipidosis, results from the defective activity of α-galactosidase A, leading to lysosomal accumulation of globotriaosylceramide and digalactosylceramide primarily in vascular endothelium, smooth-muscle cells and epithelial cells. A mouse model of FD showed accumulation of globotriaosylceramide in several organs, namely in kidneys and liver (Ohshima et al, 1997) and has been used recently to reveal a reduction in CD1d-restricted invariant natural killer T (iNKT) ...
Tópico(s): Lysosomal Storage Disorders Research
2008 - Wiley | British Journal of Haematology

Marcus Vinícius Sampaio, Giovana Matos Franco, Diego Tolentino de Lima, Amanda Rosa Custódio de Oliveira, Patrícia F. Silva, Ana Letícia Zéro dos Santos, A V M Resende, Fernando Santos, Lúcio Vilela Carneiro Girão,
Tópico(s): Geochemistry and Elemental Analysis
2020 - Springer Nature | Neotropical Entomology

Jonatas Brito de Alencar Neto, DIEGO FRADE BERNARDES, Clodoaldo José Duarte de Souza, Marcos Antônio Silva Girão, Pedro Henrique Messias da Rocha, Fernando Antônio Mendes Façanha Filho,
To evaluate the results obtained in the repair of distal biceps injury using the single-incision approach with endobutton use; complications; and ability to return to sport.
Tópico(s): Shoulder Injury and Treatment
2021 - Brazilian Society of Orthopedics and Traumatology | Acta Ortopédica Brasileira

Tatiana M. Tripoli, Hélio Sato, Marair Grácio Ferreira Sartori, Fábio Fernando de Araújo, Manoel João Batista Castelo Girão, Eduardo Schor,
Chronic pelvic pain (CPP) is one of the most frequent symptoms in women of reproductive age. This is an enigmatic clinical condition that results from the complex interactions of physiological and psychological factors with direct impact on the social, marital, and professional lives of women.To evaluate the quality of life and sexual satisfaction of women who suffer from CPP with or without endometriosis.Forty-nine patients who had been diagnosed with endometriosis and 35 patients with CPP diagnosed ...
Tópico(s): Pregnancy-related medical research
2010 - Elsevier BV | The Journal of Sexual Medicine

Bianca N.M. Silva, Policarpo Ademar Sales, Álvaro J. Romanha, Silvane Maria Fonseca Murta, Camilo Henrique da Silva Lima, Magaly Girão Albuquerque, Eliane D’Elia, José Guilherme A. Rodrigues, Vı́tor F. Ferreira, Fernando de Carvalho da Silva, Ângelo C. Pinto, Bárbara V. Silva,
Chagas disease, also known as American trypanosomiasis, is classified as one of the 17 most important neglected diseases by the World Health Organization. The only drugs with proven efficacy against Chagas disease are benznidazole and nifurtimox, however both show adverse effects, poor clinical efficacy, and development of resistance. For these reasons, the search for new effective chemical entities is a challenge to research groups and the pharmaceutical industry.Synthesis and evaluation of antitrypanosomal ...
Tópico(s): Bioactive Compounds and Antitumor Agents
2018 - Bentham Science Publishers | Medicinal Chemistry
Marieta Maria Martins Vieira, Magno José Duarte Cândido, Marco Aurélio Delmondes Bomfim, L. S. Severino, Jorge Fernando Fuentes Zapata, Liandro Torres Beserra, Abner José Girão Meneses, Joana Paula Belém Fernandes,
Objetivou-se avaliar o efeito da substituicao do farelo de soja pelo farelo de mamona destoxificado sobre as caracteristicas da carcaca e dos componentes nao-carcaca. Foram utilizados 20 borregos, machos, com peso vivo de 19,3kg e 7 meses de idade, distribuidos aleatoriamente em quatro tratamentos de 0, 50, 75 ou 100% de substituicao, com base na materia seca. O volumoso utilizado foi o feno de capim-elefante. As racoes foram fornecidas diariamente em duas refeicoes e coletaram-se, no dia seguinte, ...
Tópico(s): Animal Nutrition and Physiology
2010 - UNIVERSIDADE FEDERAL DA BAHIA | Revista Brasileira de Saúde e Produção Animal
Şule Canberk, Helena Barroca, Inês Girão, Özlem Aydın, Aysun Uğuz, Kıvılcım Eren Erdoğan, Ebru Taştekin, Massimo Bongiovanni, Paula Soares, Valdemar Máximo, Fernando Schmitt,
Background: To evaluate the performance of TBSRTC through multi-institutional experience in the paediatric population and questioning the management recommendation of ATA Guidelines Task Force on Paediatric Thyroid Cancer; Methods: A retrospective search was conducted in 4 institutions to identify consecutive thyroid FNAC cases in paediatric population between 2000 and 2018. Following the 2nd TBSRTC, the risk of malignancy ratios (ROMs) was given in ranges and calculated by 2 different ways. Sensitivity, ...
Tópico(s): Thyroid Cancer Diagnosis and Treatment
2022 - Multidisciplinary Digital Publishing Institute | Diagnostics

Lísia Miglioli-Galvão, José Osmar Medina Pestana, Guilherme Santoro‐Lopes, Renato Torres Gonçalves, Lúcio Requião‐Moura, Álvaro Pacheco Silva, Lígia Câmera Pierrotti, Elias David‐Neto, Evelyne Santana Girão, Cláudia Maria Costa de Oliveira, Cely Saad Abboud, João Ítalo Dias França, Carolina Devite Bittante, Luci Corrêa, Luís Fernando Aranha Camargo,
Background Severe Strongyloides stercoralis infection in kidney transplant recipients is associated with considerable morbidity and mortality, although little is known about the risk factors for such infection. Methodology/Principal findings This was a retrospective, multicenter, case–control study in which we assessed the risk factors for and clinical outcomes of severe S. stercoralis infections in kidney transplant recipients in Brazil. We included 138 kidney transplant recipients: 46 cases and ...
Tópico(s): Parasitic infections in humans and animals
2020 - Public Library of Science | PLoS neglected tropical diseases

Mônica Carolina Miranda, Fernando José de Nóbrega, Kazue Sato, Sabine Pompéia, Elaine Girão Sinnes, Orlando F. A. Bueno,
OBJECTIVES: to compare the neuropsychological profile of eutrophic, stunted and chronically malnourished children, and to analyse the role of socio-economic factors on the cognitive development. METHODS: seven to 10 year-old girls and boys from a poor community in São Paulo were evaluated: 27 eutrophyc, 31 stunted and 15 chronically malnourished. Neuropsychological evaluation involved cognitive functions not fully assessed in this population, such as working, declarative and non-declarative memories, ...
Tópico(s): Child Nutrition and Feeding Issues
2007 - Instituto Materno Infantil de Pernambuco | Revista Brasileira de Saúde Materno Infantil

Stênio Fernando Pimentel Duarte, Emílio Antônio Francischetti, V. Genelhu-Abreu, Sérgio Girão Barroso, José Ueleres Braga, Pedro Hernán Cabello, Márcia Mattos Gonçalves Pimentel,
Abstract Several genes play a major role in obese phenotypes, and studies suggest that genetic variations among individuals, as well as their lifestyles, may bring about different body compositions. Among these genes, LEP , which codifies leptin, and the LEPR gene encoding its receptor were extensively studied for variants that could explain the obese phenotype. The LEPR p.Q223R gene polymorphism was analyzed in a sample of obese and nonobese individuals from Brazil to evaluate the role of this polymorphism ...
Tópico(s): Adipokines, Inflammation, and Metabolic Diseases
2006 - Wiley | American Journal of Human Biology