Celia Andrés, José Manuel Pérez de la Lastra, Francisco J. Plou, Eduardo Pérez‐Lebeña,
Living species are continuously subjected to all extrinsic forms of reactive oxidants and others that are produced endogenously. There is extensive literature on the generation and effects of reactive oxygen species (ROS) in biological processes, both in terms of alteration and their role in cellular signaling and regulatory pathways. Cells produce ROS as a controlled physiological process, but increasing ROS becomes pathological and leads to oxidative stress and disease. The induction of oxidative ...
Tópico(s): Nitric Oxide and Endothelin Effects
2021 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
B. Berenguer, Catalina Alarcón‐de‐la‐Lastra, Francisco J. Moreno, M.J. Martín,
The influence of different nonsteroidal anti-inflammatory drugs (NSAIDs) and of a proton pump inhibitor on the healing parameters of a chronic gastric ulcer was evaluated. Wistar rats were used after the induction of a chronic acetic acid ulcer. The animals were treated orally for 8 and 15 days, twice daily, with the conventional NSAID, piroxicam (0.35 mg/kg), the non-narcotic analgesic, metamizol (33 mg/kg), the selective cyclooxygenase-2 inhibitor, celecoxib (1.8 mg/kg) and the proton pump inhibitor, ...
Tópico(s): Asthma and respiratory diseases
2002 - Elsevier BV | European Journal of Pharmacology
Isabel Álvarez‐Laderas, Teresa Lopes Ramos, Mayte Medrano, Rocío Caracuel-García, María Victoria Barbado, Marina Sánchéz‐Hidalgo, Rosario Zamora, Catalina Alarcón‐de‐la‐Lastra, Francisco J. Hidalgo, José I. Piruat, Teresa Caballero‐Velázquez, José Antonio Pérez‐Simón,
Polyphenols are a group of chemical substances found in plants, with immunomodulatory, antiproliferative, and anti-inflammatory properties that might be useful in the prophylaxis and treatment of graft-versus-host disease (GVHD). Polyphenolic extract (PE) obtained from extra virgin olive oil (EVOO) decreased the activation and proliferation of activated T cells. In addition, a decreased production of proinflammatory cytokines was observed upon exposure to PE. Western blot assays showed a marked inhibition ...
Tópico(s): T-cell and B-cell Immunology
2019 - Elsevier BV | Biology of Blood and Marrow Transplantation
Celia María Curieses Andrés, José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
Free radicals (FRs) are unstable molecules that cause reactive stress (RS), an imbalance between reactive oxygen and nitrogen species in the body and its ability to neutralize them. These species are generated by both internal and external factors and can damage cellular lipids, proteins, and DNA. Antioxidants prevent or slow down the oxidation process by interrupting the transfer of electrons between substances and reactive agents. This is particularly important at the cellular level because oxidation ...
Tópico(s): Antioxidant Activity and Oxidative Stress
2024 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Celia María Curieses Andrés, José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
This review focuses on DNA damage caused by a variety of oxidizing, alkylating, and nitrating species, and it may play an important role in the pathophysiology of inflammation, cancer, and degenerative diseases. Infection and chronic inflammation have been recognized as important factors in carcinogenesis. Under inflammatory conditions, reactive oxygen species (ROS) and reactive nitrogen species (RNS) are generated from inflammatory and epithelial cells, and result in the formation of oxidative and ...
Tópico(s): Carcinogens and Genotoxicity Assessment
2023 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Celia María Curieses Andrés, José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
Classically, superoxide anion O
Tópico(s): Vitamin C and Antioxidants Research
2023 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Celia María Curieses Andrés, José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
The influence of modern lifestyle, diet, exposure to chemicals such as phytosanitary substances, together with sedentary lifestyles and lack of exercise play an important role in inducing reactive stress (RS) and disease. The imbalance in the production and scavenging of free radicals and the induction of RS (oxidative, nitrosative, and halogenative) plays an essential role in the etiology of various chronic pathologies, such as cardiovascular diseases, diabetes, neurodegenerative diseases, and cancer. ...
Tópico(s): Free Radicals and Antioxidants
2023 - Wiley | Journal of Biochemical and Molecular Toxicology
Celia María Curieses Andrés, José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
Hydrogen sulfide (H
Tópico(s): Neuroscience of respiration and sleep
2023 - Multidisciplinary Digital Publishing Institute | Cells
Celia María Curieses Andrés, José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
In this review, we have focused mainly on the study of their antioxidant and pro-oxidant capacity, and the analysis of the oxidation of the catechol group to o-quinone. The redox balance established between the different oxidase and reductase enzymes generates reducing species, H+ and e−, and allows the oxidation of polyphenolic groups to quinones to be reversible. This continuous balance between these nucleophilic and electrophilic substances allows the activation of the NRF2/ARE axis, which regulates ...
Tópico(s): Genomics, phytochemicals, and oxidative stress
2023 - Multidisciplinary Digital Publishing Institute | Processes
José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
In recent years, much interest has been generated by the idea that nitrosative stress plays a role in the aetiology of human diseases, such as atherosclerosis, inflammation, cancer, and neurological diseases. The chemical changes mediated by reactive nitrogen species (RNS) are detrimental to cell function, because they can cause nitration, which can alter the structures of cellular proteins, DNA, and lipids, and hence, impair their normal function. One of the most potent biological nitrosative agents ...
Tópico(s): Neutrophil, Myeloperoxidase and Oxidative Mechanisms
2022 - Multidisciplinary Digital Publishing Institute | Stresses
Celia María Curieses Andrés, José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
This review examines the impact of reactive species RS (of oxygen ROS, nitrogen RNS and halogens RHS) on various amino acids, analyzed from a reactive point of view of how during these reactions, the molecules are hydroxylated, nitrated, or halogenated such that they can lose their capacity to form part of the proteins or peptides, and can lose their function. The reactions of the RS with several amino acids are described, and an attempt was made to review and explain the chemical mechanisms of the ...
Tópico(s): Vitamin C and Antioxidants Research
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Celia María Curieses Andrés, José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
Hydrogen peroxide (H2O2) is a compound involved in some mammalian reactions and processes. It modulates and signals the redox metabolism of cells by acting as a messenger together with hydrogen sulfide (H2S) and the nitric oxide radical (•NO), activating specific oxidations that determine the metabolic response. The reaction triggered determines cell survival or apoptosis, depending on which downstream metabolic pathways are activated. There are several ways to produce H2O2 in cells, and cellular systems ...
Tópico(s): Sulfur Compounds in Biology
2022 - Multidisciplinary Digital Publishing Institute | Stresses
Celia María Curieses Andrés, José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
This review discusses the formation of hypochlorous acid HOCl and the role of reactive chlorinated species (RCS), which are catalysed by the enzyme myeloperoxidase MPO, mainly located in leukocytes and which in turn contribute to cellular oxidative stress. The reactions of RCS with various organic molecules such as amines, amino acids, proteins, lipids, carbohydrates, nucleic acids, and DNA are described, and an attempt is made to explain the chemical mechanisms of the formation of the various chlorinated ...
Tópico(s): Nitric Oxide and Endothelin Effects
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Celia María Curieses Andrés, José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
This review examines the role of chlorine dioxide (ClO2) on inorganic compounds and cell biomolecules. As a disinfectant also present in drinking water, ClO2 helps to destroy bacteria, viruses, and some parasites. The Environmental Protection Agency EPA regulates the maximum concentration of chlorine dioxide in drinking water to be no more than 0.8 ppm. In any case, human consumption must be strictly regulated since, given its highly reactive nature, it can react with and oxidize many of the inorganic ...
Tópico(s): Chemical Analysis and Environmental Impact
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Celia Andrés, José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
This review examines the role of reactive species RS (of oxygen ROS, nitrogen RNS and halogen RHS) on innate immunity. The importance of these species in innate immunity was first recognized in phagocytes that underwent a “respiratory burst” after activation. The anion superoxide •O2− and hydrogen peroxide H2O2 are detrimental to the microbial population. NADPH oxidase NOx, as an •O2− producer is essential for microbial destruction, and patients lacking this functional oxidase are more susceptible to ...
Tópico(s): Immune Response and Inflammation
2022 - Multidisciplinary Digital Publishing Institute | Vaccines
R. Reyes-García, José Manuel Pérez de la Lastra, Joaquín Vicente, Francisco Ruiz‐Fons, Joseba M. Garrido, Christian Gortázar,
A role of wildlife species as paratuberculosis reservoirs is strongly suspected based on field and molecular epidemiologic evidence. This paper presents the first large-scale data on enzyme-linked immunosorbent assay (ELISA) against Mycobacterium avium subspecies paratuberculosis (MAP) antibodies in red deer from Spain, and tests the effect of host and environmental risk factors on antibody levels. A total of 257 out of 852 serum samples tested positive, yielding a total seroprevalence of 30.16% (95% ...
Tópico(s): Tuberculosis Research and Epidemiology
2008 - Elsevier BV | Veterinary Immunology and Immunopathology
Diana Carreón, José Manuel Pérez de la Lastra, Consuelo Almazán, Mario Canales, Francisco Ruiz‐Fons, Mariana Boadella, Juan A. Moreno-Cid, Margarita Villar, Christian Gortázar, Manuel M. Reglero, Ricardo Villarreal, José de la Fuente,
Red deer (Cervus elaphus) and white-tailed deer (Odocoileus virginianus) are hosts for different tick species and tick-borne pathogens and play a role in tick dispersal and maintenance in some regions. These factors stress the importance of controlling tick infestations in deer and several methods such as culling and acaricide treatment have been used. Tick vaccines are a cost-effective alternative for tick control that reduced cattle tick infestations and tick-borne pathogens prevalence while reducing ...
Tópico(s): Vector-Borne Animal Diseases
2011 - Elsevier BV | Vaccine
José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
SARS-CoV-2, the coronavirus triggering the disease COVID-19, has a catastrophic health and socioeconomic impact at a global scale. Three key factors contribute to the pathogenesis of COVID-19: excessive inflammation, immune system depression/inhibition, and a set of proinflammatory cytokines. Common to these factors, a central function of oxidative stress has been highlighted. A diversity of clinical trials focused predominantly on antioxidants are being implemented as potential therapies for COVID- ...
Tópico(s): Vitamin C and Antioxidants Research
2021 - Multidisciplinary Digital Publishing Institute | Processes
Carolina Velázquez, Enrique Lastra, Francisco Avila Cobos, L. Abella, Virginia de la Cruz, Blanca Hernando, Lara Hernández, Noemí Martínez, Mar Infante, M. Durán,
Abstract Background In the context of our Regional Program of Hereditary Cancer, individuals fulfilling the criteria are tested for germline mutations to subsequently establish the clinical management. Our standard diagnostic approach focuses on sequencing a few classic high-risk genes, a method that frequently renders uninformative genetic results. This study aims to examine the improved yield offered by an On-Demand panel. Methods We designed an On-Demand panel for the analysis of 35-genes associated ...
Tópico(s): CRISPR and Genetic Engineering
2020 - BioMed Central | Journal of Translational Medicine
Eduardo de la Lastra, José Ignacio Marín-Guirao, Francisco Javier López‐Moreno, Teresa Soriano, Miguel de Cara García, Nieves Capote,
Abstract BACKGROUND Asparagus decline syndrome (ADS), one of the most important diseases affecting asparagus crops, causes important yield losses worldwide. Fusarium proliferatum , F. oxysporum and F. redolens are among the main species associated with ADS. To explore their potential inoculum sources and the effectiveness of soil disinfestation practices for ADS management, molecular methods based on a quantitative real‐time polymerase chain reaction (qPCR) were developed. qPCR‐based molecular tools ...
Tópico(s): Plant Disease Resistance and Genetics
2021 - Wiley | Pest Management Science
Marina Aparicio‐Soto, Sergio Montserrat‐de la Paz, Marina Sánchéz‐Hidalgo, Ana Cárdeno, Beatriz Bermúdez, Francisco J.G. Muriana, Catalina Alarcón‐de‐la‐Lastra,
Abstract Monocytes and macrophages are critical effectors and regulators of inflammation and innate immune response, which appear altered in different autoimmune diseases such as systemic lupus erythematosus (SLE). Recent studies suggested that virgin olive oil (VOO) and particularly its phenol compounds might possess preventive effects on different immune-inflammatory diseases, including SLE. Here, we evaluated the effects of VOO (and sunflower oil) on lipopolysaccharide (LPS)-activated peritoneal ...
Tópico(s): Cholesterol and Lipid Metabolism
2018 - Cambridge University Press | British Journal Of Nutrition
Ana Cárdeno, Marina Aparicio‐Soto, Sergio Montserrat‐de la Paz, Beatriz Bermúdez, Francisco J.G. Muriana, Catalina Alarcón‐de‐la‐Lastra,
Tópico(s): Antioxidant Activity and Oxidative Stress
2015 - Elsevier BV | Journal of Functional Foods
Marina Gómez, Francisco Barreiro, J. López, Mariano Lastra, Rosario de la Huz,
Wrack supply is a common feature of beaches around the world. The species composition and spatial distribution of wrack deposits have been widely recognized as two significant factors in the ecological role of macroalgae within beach ecosystems. These accumulation processes may be intensified by bloom events of opportunistic algae, which frequently occur in estuarine environments. For this study, wrack supply was assessed over 13 months in two estuarine beaches of the Galician coastline, NW Spain. ...
Tópico(s): Marine Biology and Environmental Chemistry
2012 - Elsevier BV | Aquatic Botany
José Manuel Pérez de la Lastra, Ruth C. Galindo, Christian Gortázar, Francisco Ruiz‐Fons, Alicia Aranaz, J DELAFUENTE,
The objective of this study was to analyze the expression of immunoregulatory genes in European wild boar (Sus scrofa) immunized with BCG. Eighteen immunoregulatory genes were selected for expression analysis based on their role in host immune response during tuberculosis and/or for their association with resistance to bovine tuberculosis in European wild boar populations. Initially, mRNA levels were analyzed by quantitative real-time reverse transcription PCR (qRT-PCR) in spleen samples from Mycobacterium ...
Tópico(s): Immune Response and Inflammation
2008 - Elsevier BV | Veterinary Microbiology
José Luís Ayuso‐Mateos, Francisco Montañés Rada, I. Lastra, Juan J. Picazo De La Garza, J.L. Ayuso-Gutiérrez,
Background Recent surveys suggest that psychiatric patients are at increased risk of being infected with HIV, although very little information is available concerning the seroprevalence of HIV infection among this population outside the US. The aim of this study is to determine the seroprevalence of HIV-I among patients admitted to a psychiatric in-patient unit and to gather linked anonymous risk-factor information. Method An unlinked serosurvey was made, using HIV-1 antibody testing of remnant ...
Tópico(s): HIV Research and Treatment
1997 - Cambridge University Press | The British Journal of Psychiatry
Celia Andrés, José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
Myeloid-derived suppressor cells MDSCs are a heterogeneous population of cells that expand beyond their physiological regulation during pathologies such as cancer, inflammation, bacterial, and viral infections. Their key feature is their remarkable ability to suppress T cell and natural killer NK cell responses. Certain risk factors for severe COVID-19 disease, such as obesity and diabetes, are associated with oxidative stress. The resulting inflammation and oxidative stress can negatively impact ...
Tópico(s): Inflammatory Biomarkers in Disease Prognosis
2023 - Multidisciplinary Digital Publishing Institute | Vaccines
Francisco Barreiro, Marina Gómez, J. López, Mariano Lastra, Rosario de la Huz,
Tópico(s): Coastal wetland ecosystem dynamics
2012 - Springer Science+Business Media | Hydrobiologia
Francisco Barreiro, Marina Gómez, Mariano Lastra, J. López, Rosario de la Huz,
MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections MEPS 433:65-74 (2011) - DOI: https://doi.org/10.3354/meps09130 Annual cycle of wrack supply to sandy beaches: effect of the physical environment F. Barreiro*, M. Gómez, M. Lastra, J. López, R. de la Huz Departamento de Ecoloxía e Bioloxía Animal, Facultade de Ciencias, Universidade de Vigo, 36310 Vigo, Spain *Email: ...
Tópico(s): Marine and coastal plant biology
2011 - Inter-Research | Marine Ecology Progress Series
Celia María Curieses Andrés, José Manuel Pérez de la Lastra, Elena Bustamante Munguira, Celia Andrés, Francisco J. Plou, Eduardo Pérez Lebeña,
The phrase “Let food be thy medicine…” means that food can be a form of medicine and medicine can be a form of food; in other words, that the diet we eat can have a significant impact on our health and well-being. Today, this phrase is gaining prominence as more and more scientific evidence suggests that one’s diet can help prevent and treat disease. A diet rich in fruits, vegetables, whole grains, and lean protein can help reduce the risk of heart disease, cancer, diabetes, and other health problems ...
Tópico(s): Free Radicals and Antioxidants
2024 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
José Manuel Pérez de la Lastra, Celia Andrés, Francisco J. Plou, Eduardo Pérez‐Lebeña,
Vitamin E and dietary flavonoids are natural substances with antioxidant and anti-inflammatory activities, showing little or no side effects. Fruit and vegetable diets based on flavonoids and vitamin E provide a benefit to hypertensive subjects by regulating blood pressure. However, the exact mechanism of their anti-inflammatory properties has not been chemically explained. It has been proposed that their anti-oxidant and anti-inflammatory properties may be related to their ability to scavenge free ...
Tópico(s): Antioxidant Activity and Oxidative Stress
2022 - Multidisciplinary Digital Publishing Institute | Stresses