Cant, G. Carlisle. 12 November 1888
0000 - Gale Group | NCCO BritishPolitics
... protein families (G(s), G(i), G(q), G(12)). The G protein coupling pattern links GPCR activation to the specific downstream effector pathways. G(12/13) signalling of GPCRs has been studied only ... exchange factors (RhoGEFs). Four mammalian RhoGEFs regulated by G(12/13) proteins are known: p115-RhoGEF, PSD-95/ ... monomeric GTPase RhoA, and other downstream effectors. Misregulated G(12/13) signalling is involved in multiple pathophysiological conditions ... pulmonary hypertension, and bronchial asthma. Specific targeting of G(12/13) signalling-related diseases of GPCRs hence provides ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2009 - Wiley | British Journal of Pharmacology
Cant, G. Carlisle. 12 November 1888
0000 - Gale Group | NCCO BritishPolitics
D PATEL, Lawrence E. Shapiro, Sharon A. Kozlowski, Barbara L. Gaffney, Sandra Kuzmich, Roger A. Jones,
... 12-mer et G3 dans celle de O6meG.G 12-mer, qui sont associées au site de modification, ... sauf pour le O6meG10 dans le duplex O6meG.G 12-mer qui, lui, adopte une configuration syn. Ceci ... C9 qui l'encadrent dans le duplex O6meG.G 12-mer. Nous observons un renversement du profil pour ... phosphore des duplex O6meG.C 12-mer, O6meG.G. 12-mer et O6meG.A 12-mer, qui exhibent ... T 12-mer and G3 in the O6meG.G 12-mer helix, which are associated with the modification ... modification site except for O6meG10 in the O6meG.G 12-mer duplex which adopts a syn configuration. This ...
Tópico(s): DNA Repair Mechanisms
1985 - Elsevier BV | Biochimie
Cant, G. Carlisle. 12 November 1888
0000 - Gale Group | NCCO BritishPolitics
Myriam Grimm, Denise Tischner, Kerstin Troidl, Julián Albarrán-Juárez, Kishor K. Sivaraj, Nerea Ferreirós Bouzas, Gerd Geißlinger, Christoph J. Binder, Nina Wettschureck,
... pathogenesis of atherosclerosis. Heterotrimeric G proteins of the G 12/13 family have been implicated in the control ... and Results— Mice with myeloid-specific deficiency for G 12/13 show reduced atherosclerosis with a clear shift ... activation of inflammatory gene expression; on the contrary, G 12/13 -deficient macrophages show an increased nuclear factor- ... in resident peritoneal macrophages results in myeloid-specific G 12/13 -deficient mice in an altered peritoneal micromilieu ... type mice. With respect to the mechanism of G 12/13 -mediated transcriptional control, we identify an autocrine ...
Tópico(s): Galectins and Cancer Biology
2015 - Lippincott Williams & Wilkins | Arteriosclerosis Thrombosis and Vascular Biology
... 39): Economic elimination. Eyewitness Accounts. Buckwitz, Dr. Hans G. 12-Nov-54. 1. 047-EA-0490. German. P. ...
1954 - Gale Group | ArchivesUnbound Holocaust Rare
Yoshihiro Nakagawa, Hiroyuki Hayashi, Takahiko Tawaratani, Hiroki Kourai, Tokunaru Hòrie, Isao Shibasaki,
... glass showed antibacterial activity. An agent designated as G 12 , which had a dodecyl alkyl chain, was selected ... the agent reported previously. The antibacterial activity of G 12 toward Escherichia coli was mainly due to the ... continuous treatment of a cell suspension. The lost G 12 activity was completely recovered by washing with ethanol, and the activity of refreshed G 12 decreased in the same manner as that of fresh G 12 . The lost activity was, however, always recovered only by ethanol treatment. This indicated that G 12 might interact with cells more strongly by means ...
Tópico(s): Surfactants and Colloidal Systems
1984 - American Society for Microbiology | Applied and Environmental Microbiology
... April. Letter. From Sidmouth. To Earl Spencer K. G. 12. Gloucester Place.
0000 - Gale Group | NCCO BritishPolitics
Yoshifumi Kawanabe, Yasuo Okamoto, Soichi Miwa, Nobuo Hashimoto, Tomoh Masaki,
... reported that CHO-ET A R couples with G 12 in addition to G q and G s . ... coupling to either G q or G s /G 12 (designated ET A RΔ385 and SerET A R, respectively) and a dominant-negative mutant of G 12 (G 12 G228A). ET A RΔ385 is truncated immediately downstream ... 1–stimulated CHO-SerET A R. Microinjection of G 12 G228A abolished the activation of NSCC-1 and ... related pathways. NSCC-1 is activated via a G 12 -dependent pathway, NSCC-2 via G q /PLC- and G 12 -dependent pathways, and SOCC via a G q / ...
Tópico(s): Receptor Mechanisms and Signaling
2002 - American Society for Pharmacology and Experimental Therapeutics | Molecular Pharmacology
Bruno Meyer, Felix Freuler, Danilo Guerini, Sandra Siehler,
... families, G s , G i , G q , and G 12 , engage in their linkage to activation of receptor‐specific signal transduction pathways. G 12 proteins were more recently studied, and upon activation ... and physiological aspects, and a dysfunction of the G 12/13 ‐Rho pathway can lead to hypertension, cardiovascular ... plasma membrane was observed upon activation of several G 12/13 ‐coupled GPCRs in a cell type‐independent ... to the cytosol. The translocation of RhoGEF by G 12/13 ‐linked GPCRs can be quantified and therefore ...
Tópico(s): PI3K/AKT/mTOR signaling in cancer
2008 - Wiley | Journal of Cellular Biochemistry
Natalia A. Riobo‐Del Galdo, David R. Manning,
Much regarding the engagement of the G(12) family of heterotrimeric G proteins (G(12) and G(13)) by agonist-activated receptors remains unclear. For example, the identity of receptors that couple unequivocally to G(12) and G(13) and how signals are allocated ... Part of the problem in understanding signaling through G(12) and G(13) is that the activation of ... removed downstream events. Furthermore, receptors that couple to G(12) and G(13) invariably couple to additional G ... article, we document receptors that reportedly couple to G(12), G(13) or both G(12) and G( ...
Tópico(s): Cell Adhesion Molecules Research
2005 - Elsevier BV | Trends in Pharmacological Sciences
Dinshaw J. Patel, Lawrence Shapiro, Sharon A. Kozlowski, Barbara L. Gaffney, Roger A. Jones,
... A 12-mer when N3 = A3 and O6meG·G 12-mer when N3 = G3), which contain symmetry-related ... in both O6meG·A 12-mer and O6meG·G 12-mer duplexes. We have assigned all the base ... the O6meG·A 12-mer duplex and O6meG·G 12-mer duplex in solution. The observed NOEs establish ... the glycosidic bond in the right-handed O6meG·G 12-mer duplex. This results in altered NOE patterns ... flanking C9 and C11 residues in the O6meG·G 12-mer duplex. The phosphorus backbone is perturbed at ... over 1 part per million in the O6meG·G 12-mer compared to a 0·5 part per ...
Tópico(s): RNA modifications and cancer
1986 - Elsevier BV | Journal of Molecular Biology
Yoshifumi Kawanabe, Yasuo Okamoto, Kazuhiko Nozaki, Nobuo Hashimoto, Soichi Miwa, Τοmoh Masaki,
... C (PLC) inhibitor, or dominant negative mutants of G 12 (G 12 G228A) or G 13 (G 13 G225A), whereas it was inhibited by U73122 in combination with G 12 G228A but not G 13 G225A. Dibutyryl cAMP ... ET A Rs, the formation was sensitive to G 12 G228A or U73122, respectively. These results indicate that ... is essential for coupling with G s and G 12 , and 3) the signal for ET-1–induced ... is transmitted through the G q /PLC- and G 12 -dependent pathway to the Rho/ROCK system.
Tópico(s): Receptor Mechanisms and Signaling
2002 - American Society for Pharmacology and Experimental Therapeutics | Molecular Pharmacology
Boris Le Nevé, Martin Foltz, Hannelore Daniel, Robin J. Gouka,
... are not fully understood. We found that H.g.-12, a steroid glycoside purified from H. gordonii extract, ... H. gordonii purified extracts, we assessed whether H.g.-12 could activate human bitter receptors. The steroid glycoside ... structural feature from the steroid glycoside inhibited H.g.-12-induced Ca²(+) increase in TAS2R14-expressing HEK cells and abolished H.g.-12-induced CCK secretion from human EC cells. Similarly, a nonspecific bitter receptor antagonist abolished H.g.-12-induced CCK secretion in HuTu-80 cells. These ...
Tópico(s): Olfactory and Sensory Function Studies
2010 - American Physiological Society | AJP Gastrointestinal and Liver Physiology
Li Zhang, Cherisse DiLizio, David Kim, Emer M. Smyth, David R. Manning,
... in the understanding of pathways regulated by the G 12 family of heterotrimeric G proteins, much regarding the ... thromboxane A 2 receptor TPα, the ability of G 12 and G 13 to report differences in the ... PGF 2α ), among other ligands examined, to activate G 12 and G 13 through TPα explicitly. We were ... functionality of TPα-Gα fusion proteins germane to G 12 and G 13 . Using fusion proteins in Spodoptera ... 12 was the reporter. These data establish that G 12 and G 13 can report differentially potency and ...
Tópico(s): Protein Kinase Regulation and GTPase Signaling
2006 - American Society for Pharmacology and Experimental Therapeutics | Molecular Pharmacology
Stefan Rieken, Susanne Herroeder, Antonia Sassmann, Barbara Wallenwein, Alexandra Moers, Stefan Offermanns, Nina Wettschureck,
... Rho and Rhokinase, indicative of an involvement of G(12)/G(13), possibly also G(q)/G(11) family G-proteins. We used G(12)/G(13)- or G(q)/G(11)-deficient ... of LPA and S1P are completely abrogated in G(12)/G(13)-deficient marginal zone B cells, reduced in G(12)/G(13)-deficient follicular B cells, and normal ... B cell populations observed in B cell-specific G(12)/G(13)-deficient mice in vivo. Taken together, ... cells to ICAM-1 through G(i) and G(12)/G(13) family G-proteins but not through ...
Tópico(s): T-cell and B-cell Immunology
2006 - Elsevier BV | Journal of Biological Chemistry
Luis P. Reynaldo, Joseph J. Villafranca, William DeW. Horrocks,
... unadenylylated enzyme, GS 0 , and fully adenylylated enzyme, GS 12 , respectively, and that there are 2.6 0. ... at site n2 for both GS 0 and GS 12 . Energy transfer measurements between the lanthanide donor‐acceptor ... K d = 0.06 0.02 μM for GS 12 . The dissociation constant for lanthanide ion binding to ... 6 0.7 μM for GS 0 and GS 12 , respectively. Competition experiments indicate that Mn 2+ affinity ... K d = 0.35 0.09 μM for GS 12 . Mn 2+ affinity at site n2 remains unchanged ( ... K d = 4.0 1.0 μM for GS 12 ). The observed divalent metal ion affinities, which are ...
Tópico(s): Enzyme Structure and Function
1996 - Wiley | Protein Science
Martin Zamykal, Tobias Martens, Jakob Matschke, Hauke S. Günther, Annegret Kathagen, Alexander Schulte, R.J. Peters, Manfred Westphal, Katrin Lamszus,
... in the brains of nude mice, and the GS-12 cell line was used to generate highly invasive ... A12 treatment inhibited the growth of U87 and GS-12 tumors by 75% and 50%, respectively. In GS-12 tumors, the invasive tumor extension and proliferation rate ... moderately reduced. Flow cytometry showed that 85% of GS-12 cells expressed IGF-1R. Activation of IGF-1R by IGF-1 and IGF-2 in GS-12 cells was blocked by IMC-A12. Both ligands stimulated GS-12 cell proliferation, and IGF-2 also stimulated migration. ...
Tópico(s): Cancer Cells and Metastasis
2014 - Oxford University Press | Neuro-Oncology
Chaolun Zheng, Jian Zhou, Yong Pei, Bao Yang,
... and between the ionic liquid and water molecules ( $${g}_{12}$$ g 12 ) for the [EMIM][MeSO 3 ]/water binary system ... between the [EMIM][MeSO 3 ] and water molecules ( $${g}_{12}$$ g 12 ) was found to be in the range of ... the [EMIM][MeSO 3 ]/water system. The large ( $${g}_{12}$$ g 12 ) can explain many observed macroscopic thermodynamic properties such ...
Tópico(s): Thermodynamic properties of mixtures
2020 - Nature Portfolio | Scientific Reports
Guillermo Urrutia, Thiago M. de Assuncao, Angela Mathison, Ann Salmonson, Romica Kerketta, Atefeh Zeighami, Timothy J. Stodola, Volkan Adsay, Burçin Pehlivanoğlu, Michael B. Dwinell, Michael T. Zimmermann, Juan Iovanna, Raúl Urrutia, Gwen Lomberk,
... in pancreatic cells reduces H3K9me2 and antagonizes Kras G 12 D -mediated acinar-to-ductal metaplasia (ADM) and ... both the Pdx1-Cre and P48 Cre/+ Kras G 12 D mouse models. Ex vivo acinar explants also ... pathway-mediated ADM upon EHMT2 deletion. Notably, Kras G 12 D increases EHMT2 protein levels and EHMT2-EHMT1- ... p21-Chek2 pathway. Congruently, pancreas tissue from Kras G 12 D animals with EHMT2 inactivation have increased P21 ... reduces inflammatory cell infiltration typically induced during Kras G 12 D -mediated initiation. The inhibitory effect on Kras ...
Tópico(s): Pancreatic function and diabetes
2021 - Frontiers Media | Frontiers in Cell and Developmental Biology
Chris E. Cooper, J.C. Salerno,
... cytochrome oxidase exhibits a characteristic EPR signal at g' = 12. Following the (rapid) dithionite reduction of heme a ... decays at the same slow rate as the g' = 12 signal. In the fully oxidized slow enzyme the ... dependence and microwave power saturation characteristics to the g' = 12 signal. We conclude that the signal arises from ... same population of binuclear centers responsible for the g' = 12 signal. The appearance of a signal at g' = ... not prove, the model of Hagen where the g' = 12 signal arises from a ferryl heme a3, with ...
Tópico(s): Electrochemical sensors and biosensors
1992 - Elsevier BV | Journal of Biological Chemistry
Rohini Kuner, Jakub M. Swiercz, Alexandra Zywietz, Anke Tappe, Stefan Offermanns,
... or dendritic spine formation. G‐proteins of the G 12 ‐family, G 12 and G 13 , couple G‐protein‐coupled receptors ( ... to interact with the activated α‐subunits of G 12 /G 13 and are thus believed to mediate ... studies in neuronal cell lines have shown that G 12 /G 13 and PDZ‐RhoGEF mediate GPCR‐induced ... nearly ubiqoutous. Our data provide evidence that the G 12/13 –RhoGEF‐mediated pathway is present throughout the ...
Tópico(s): Neuroscience and Neuropharmacology Research
2002 - Wiley | European Journal of Neuroscience
Massimiliano Monticone, Emanuela Biollo, Massimo Maffei, Alessandra Donadini, Francesco Romeo, Clelia Tiziana Storlazzi, Walter Giaretti, Patrizio Castagnola,
... Gene expression profiles for control KRAS WT , KRAS G 12 V and KRAS G 12 D transfected cells were obtained after cell clone ... microarray data. Results We found that the KRAS G 12 V state deregulated several genes associated to cell ... respect to the KRAS WT state. The KRAS G 12 D state was, instead, characterized by several other ... the same time, the presence of the KRAS G 12 V mutation in the cells escaping apoptosis and ...
Tópico(s): Enzyme Structure and Function
2008 - BioMed Central | Molecular Cancer
Swaminathan Murugappan, Ramya Chari, Vasu M. Palli, Jianguo Jin, Satya P. Kunapuli,
... present study, we show that G(q) and G(12/13) pathways regulate the Thr(505) and Tyr( ... 311), phosphorylation on PKCdelta, whereas selective activation of G(12/13) pathways by the YFLLRNP peptide failed to ... SFKs (Src family tyrosine kinases) is essential for G(12/13)-mediated Tyr(311) phosphorylation of PKCdelta. These ... even upon stimulation of both G(q) and G(12/13) pathways. We conclude that (i) PKCdelta binding ... its translocation results in Thr(505) phosphorylation, (ii) G(12/13) pathways activate SFKs required for the phosphorylation ...
Tópico(s): Platelet Disorders and Treatments
2008 - Portland Press | Biochemical Journal
Antonio J. Pierik, Hans Wassink, H. Haaker, Wilfred R. Hagen,
... potentials around −100 mV a very low‐intensity g = 12 EPR signal was discovered. In parallel‐mode EPR ... in intensity which allowed us to assign the g = 12 signal to a non‐Kramers system (presumably S = ... metalloproteins and inorganic compounds, the sharp absorption‐shaped g = 12 signal is not significantly broadened into zero field, ... band microwave quantum. The temperature dependence of this g = 12 EPR signal indicates that it is from an ... 30 mV midpoint potentials is found for the g = 12 EPR signal. We propose that this signal represents ...
Tópico(s): Metalloenzymes and iron-sulfur proteins
1993 - Wiley | European Journal of Biochemistry
Thomas E. Meigs, Juhi Juneja, C. Todd DeMarco, Laura N. Stemmle, Daniel D. Kaplan, Patrick J. Casey,
The heterotrimeric G protein G(12) has been implicated in such cellular regulatory processes as cytoskeletal rearrangement, cell-cell adhesion, and oncogenic transformation. Although the activated alpha-subunit of G(12) has been shown to interact directly with a ... of many of these protein-protein interactions in G(12)-mediated cell physiology are poorly understood. To begin dissecting the specific cellular pathways engaged upon G(12) activation, we produced a series of substitution mutants ... will facilitate investigations into the mechanisms through which G(12) proteins mediate diverse biological responses.
Tópico(s): Blood disorders and treatments
2005 - Elsevier BV | Journal of Biological Chemistry
A. I. Levon, Yu. V. Nosenko, V. А. Onischuk, A. A. Schevchuk, A. E. Stuchbery,
... 2n)-reaction. The results are as follows: 190Pt, g(12+)=−0.17(12), g(10−)=−0.0016(36), g(7−)=+0.62(9); 192Pt, g(12+)=−0.18(9), g(10−)=−0.0012(10), g(7−)=0.48(12); 194Pt, g(12+,new assignment)=−0.17(7), g(7−)=+0.26(8); 196Hg, g(12+and10+)=−0.19(6), g(7−)=−0.030(17); 198Hg, g(12+and10+)=−0.18(8), g(7−)=−0.033( ...
Tópico(s): Quantum Chromodynamics and Particle Interactions
2005 - Elsevier BV | Nuclear Physics A
H. Tagari, K. E. Webb, Brent Theurer, Thomas Huber, D. W. DeYoung, P Cuneo, J.E.P. Santos, J M C Simas, Mohamed Sadik, A. Alio, Óscar Moralejo Lozano, A. Delgado-Elorduy, Luiz Gustavo Nussio, Carla Maris Machado Bittar, Flávio Augusto Portela Santos,
... FAA was greater (571.2 vs. 366.4 g/12 h, SEM 51.4) in cows fed SFC. ... 3 ± 10.8 and 51.5 ± 13.2 g/12 h for cows fed SFC and SRC, respectively, ... PDV flux, but variation was greater. Mammary uptake (g/12 h) of total essential FAA was greater in ... 25.0 vs. 15.1, SEM 5.2, g/12 h for cows fed SFC or SRC, respectively, ... 187.8 vs 175.4, SEM 4.4 g/12 h in cows fed SFC and SRC, respectively, ...
Tópico(s): Genetic and phenotypic traits in livestock
2004 - Elsevier BV | Journal of Dairy Science
Anisha M. Williams-Campbell, Morse B. Solomon,
... beef stew pieces were treated with 1 00 g @ 12. GB was treated with 25, 50, 75, or 100 g @ 12 and 75 @ 6. Samples were assayed for total ... in pork I beef stew pieces using 100 g @ 12'. A reduction (2-3 log; P<O.05) ... found in GB using 50, 75, or 100 g @ 12. There was no decrease in bacteria after using 25 g @ 12. An inconsistent and diminished effect on bacteria was ...
Tópico(s): Identification and Quantification in Food
2001 - SPIE | Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE