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Primary Document -- Manuscript Acesso aberto

... 18th and 23rd March, 1944; also, G11 and G12 over Germany between 15th and 19th March, 1944. ...

1944 - Gale Group | ArchivesUnbound Western Europe

Artigo Acesso aberto Revisado por pares

Jacob E. Till, Taylor A. Black, Caren Gentile, Aseel Abdalla, Zhuoyang Wang, Hareena Sangha, Jacquelyn J. Roth, Robyn T. Sussman, Stephanie S. Yee, Mark H. O’Hara, Jeffrey C. Thompson, Charu Aggarwal, Wei‐Ting Hwang, Kojo S.J. Elenitoba‐Johnson, Erica L. Carpenter,

... patients, the variant allele fractions (VAF) of KRAS G12/13 pathogenic variants in circulating tumor DNA (ctDNA) ... patients, the variant allele fractions (VAF) of KRAS G12/13 pathogenic variants in circulating tumor DNA (ctDNA) ... of ccfDNA per milliliter of plasma. The KRAS G12/13 locus was interrogated for VAF as a ... 17Crossref Scopus (352) Google Scholar First, the KRAS G12/13 locus was pre-amplified as previously described, ... S/V and G13D using the ddPCR KRAS G12/G13 Screening Assay Kit (Bio-Rad) on either ...

Tópico(s): Single-cell and spatial transcriptomics

2021 - Elsevier BV | Journal of Molecular Diagnostics

Periodico Acesso aberto

Periódicos nacionais

Artigo Revisado por pares

Shipra Gupta, Mariyam Gauhar, Parvesh Bubber, Pratima Ray,

Abstract G12 strains are now considered to be the sixth most prevalent human rotaviruses globally. India has introduced rotavirus ... During epidemiological rotavirus surveillance in HP, predominance of G12 rotaviruses was observed. This study investigated the genetic variability and evolution of HP G12 strains ( n = 15) associated with P‐genotypes P[ ... analysis of VP7 gene revealed that all characterized G12 strains clustered in lineage‐III and diversified into ... strains may have originated from three different ancestral G12 strains. The comparative sequence analysis of HP strains ...

Tópico(s): Viral Infections and Immunology Research

2021 - Wiley | Journal of Medical Virology

Periodico Acesso aberto

Periódicos nacionais

Artigo Acesso aberto Revisado por pares

Marcin Maziarz, Anthony Federico, Jingyi Zhao, Lorena Dujmusic, Zhiming Zhao, Stefano Monti, Xaralabos Varelas, Mikel Garcia‐Marcos,

... Gα subunits: Gs, Gi/o, Gq/11, and G12/13. Artificial mutations that activate Gα subunits of ... patient tumor samples. In contrast, patient tumor-associated G12/13 mutations characterized to date lead to inactivation ... signaling. First, we found that components of a G12/13-dependent signaling cascade that culminates in activation ... transcriptional signatures in this cancer type. Among the G12/13 pathway alterations were mutations in Arg-200 ... Gα subunits: Gs, Gi/o, Gq/11, and G12/13. Artificial mutations that activate Gα subunits of ...

Tópico(s): Protein Kinase Regulation and GTPase Signaling

2020 - Elsevier BV | Journal of Biological Chemistry

Periodico Acesso aberto

Periódicos nacionais

Artigo Revisado por pares

Ainara Arana, Khuzwayo C. Jere, Chrispin Chaguza, Milagrosa Montes, Miriam Alkorta, Miren Iturriza‐Gómara, Gustavo Cilla,

G12 rotaviruses were first detected in Spain (Gipuzkoa province) in December 2004. After four years with no detections, G12 strains re-emerged in the 2010-2011 epidemic ... circulation of this genotype was observed in Gipuzkoa. G12 rotaviruses were also the dominant strains in 2011- ... detected G-type between 2010 and 2018 was G12 (29.9%) rather than G1 rotavirus (17.8%), ... two to four consecutive seasons. Overall, the circulating G12 rotavirus strains in Gipuzkoa between 2010 and 2018 ... by local evolution. Whole genome analysis of 16 G12 strains detected from 2010 to 2018 revealed a ...

Tópico(s): Animal Virus Infections Studies

2019 - Elsevier BV | Infection Genetics and Evolution

Livro

Project Gutenberg

Artigo Acesso aberto Revisado por pares

Amal Moussa, M. Ben Fredj, Meriam BenHamida-Rebaï, I. Fodha, Noureddine Boujâafar, Abdelhalim Trabelsi,

... human G-types are G1-4 and G9. G12 genotype is currently emerging worldwide, becoming the sixth ... RVA G-genotype. In Tunisia, an emergence of G12 RVA strains was observed. To understand the evolution and origin of these Tunisian G12 strains, phylogenetic analyses were conducted.A total of ... positive samples were used for the detection of G12 RVA strains by semi-nested RT-PCR. G12-positive specimens were subjected to VP4 genotyping reaction. PCR products of the G12-positive samples were sequenced and characterized by phylogenetic ...

Tópico(s): Hepatitis Viruses Studies and Epidemiology

2017 - Microbiology Society | Journal of Medical Microbiology

Artigo Acesso aberto Revisado por pares

Soochong Kim, Lina Cipolla, Gianni Francesco Guidetti, Mitsuhiko Okigaki, Jianguo Jin, Mauro Torti, Satya P. Kunapuli,

... depends on integrin signaling. Unlike ADP, thrombin activates G12/13 pathways, and G12/13 pathways can substitute for integrin signaling for ... found that Pyk2 was activated downstream of both G12/13 and integrin-mediated pathways, and both 2- ... antagonist was not rescued by co-stimulation of G12/13 pathways in the presence of Pyk2 inhibitor. ... is a common signaling effector downstream of both G12/13 and integrin αIIbβ3 signaling, which contributes to ... functional tyrosine kinase that is activated by both G12/13 and integrin αIIbβ3 in platelets.Significance:Understanding ...

Tópico(s): HER2/EGFR in Cancer Research

2013 - Elsevier BV | Journal of Biological Chemistry

Carta Acesso aberto Revisado por pares

Jonathan Levinsohn, Joyce Teng, Brittany G. Craiglow, Erin Loring, T. Burrow, Shrikant S. Mane, John D. Overton, Richard P. Lifton, Jennifer M. McNiff, Anne W. Lucky, Keith A. Choate,

... A somatic heterozygous HRAS c.34G>A, p.G12S substitution was found in each (Figure 2a). There ... from affected individual WHN101, finding the HRAS p.G12S mutation in curly hair only (Figure 2d and ... Scopus (75) Google Scholar) including the HRAS p.G12S mutation found in WHN (Hafner et al., 2011Hafner ... results from germline heterozygous HRAS mutations, including p.G12S (Gripp and Lin, 2012Gripp K.W. Lin A. ... Google Scholar). Though comparison of the WHN p.G12S mutation and the common NS p.G13R mutation ... G13R was present in 85 NS and p.G12S was not found (Supplementary Table S2 online). In ...

Tópico(s): Autoimmune Bullous Skin Diseases

2013 - Elsevier BV | Journal of Investigative Dermatology

Artigo Revisado por pares

David Featherstone, Anthony Ince, Danny MacKinnon, Kendra Strauss, Andrew Cumbers,

... Geographical and Earth Sciences, University of Glasgow, Glasgow G12 8QQ email: [email protected]Search for more papers ... Geographical and Earth Sciences, University of Glasgow, Glasgow G12 8QQ email: [email protected]Search for more papers ... Geographical and Earth Sciences, University of Glasgow, Glasgow G12 8QQ email: [email protected]Search for more papers ... Geographical and Earth Sciences, University of Glasgow, Glasgow G12 8QQ email: [email protected]Search for more papers ... Geographical and Earth Sciences, University of Glasgow, Glasgow G12 8QQ email: [email protected]Search for more papers ...

Tópico(s): Scottish History and National Identity

2012 - Wiley | Transactions of the Institute of British Geographers

Artigo Acesso aberto Revisado por pares

Dagmara Oszkiewicz, E. Bowell, L. H. Wasserman, K. Muinonen, Antti Penttilä, T. Pieniluoma, David E. Trilling, Cristina A. Thomas,

... taxonomy and photometric phase curve using the H, G12 photometric phase function, with the shape of the phase function described by the single parameter G12. We explore the usability of G12 in taxonomic classification for individual objects, asteroid families, ... groups. We conclude that the mean values of G12 for the considered taxonomic complexes are statistically different, and also discuss the overall shape of the G12 distribution for each taxonomic complex. Based on the values of G12 for about half a million asteroids, we compute ...

Tópico(s): Geology and Paleoclimatology Research

2012 - Elsevier BV | Icarus

Artigo Acesso aberto Revisado por pares

Juhi Juneja, Ian Cushman, Patrick J. Casey,

Signaling through the heterotrimeric G protein, G12, via Rho induces a striking increase in breast cancer cell invasion. In this study, evidence is provided that the c-Jun NH2- ... kinase (JNK) is a key downstream effector of G12 on this pathway. Expression of constitutively-active Gα12 or activation of G12 signaling by thrombin leads to increased JNK and ... knockdown of JNK expression by siRNA significantly decreases G12-induced JNK activation as well as the ability ... an inhibitor of the Rho kinase, ROCK, reduces G12-induced JNK and c-Jun activation, and ROCK ...

Tópico(s): PI3K/AKT/mTOR signaling in cancer

2011 - Public Library of Science | PLoS ONE

Artigo Acesso aberto Revisado por pares

Dan Groff, Peng R. Chen, Francis B. Peters, Peter G. Schultz,

... described18 resulted in the identification of the aaRS G12 (experimental details in Supporting Information), which is capable of incorporating 1 into proteins. aaRS G12 has the following mutations: Y271I, L274M, and C313A. ... tag was expressed in the presence of the G12 aaRS/ pair and 1. In the absence of ... with high fidelity in E. coli using the G12 aaRS/pair. A) E. coli amber suppression of ... 1 Myo mutant. Next we determined if the G12 aaRS/ pair could be used to selectively incorporate ... into proteins in mammalian cells. The genes encoding G12 and were introduced into the mammalian vector pCMV, ...

Tópico(s): Chemical Synthesis and Analysis

2010 - Wiley | ChemBioChem

Artigo Acesso aberto Revisado por pares

Jianguo Jin, Yingying Mao, Dafydd Thomas, Soochong Kim, James L. Daniel, Satya P. Kunapuli,

... PAR 4, and the thromboxane receptor activate the G12/13 pathways, in addition to the Gq pathways. Here, we investigated the contribution of G12/13 pathways to platelet dense granule release. 2MeSADP, which does not activate G12/13 pathways, does not cause dense granule release ... with YFLLRNP (60 μM), as selective activator of G12/13 pathways, resulted in dense granule release. Similarly, supplementing PLC activation with G12/13 stimulation also leads to dense granule release. These results demonstrate that supplemental signaling from G12/13 is required for Gq-mediated dense granule ...

Tópico(s): Blood Coagulation and Thrombosis Mechanisms

2008 - Elsevier BV | Biochemical Pharmacology

Artigo Acesso aberto Revisado por pares

Ines Macias‐Perez, Roy Zent, Monica Carmosino, Matthew D. Breyer, Richard Breyer, Ambra Pozzi,

... requires the engagement of the heterotrimeric G protein G12. Thus, our study provides strong evidence that selective ... receptor suppresses tumor cell function by activating a G12-RhoA pathway. Prostaglandin E2, which exerts its functions ... requires the engagement of the heterotrimeric G protein G12. Thus, our study provides strong evidence that selective ... receptor suppresses tumor cell function by activating a G12-RhoA pathway. Colorectal cancer is the second most ... decreases cell proliferation. These effects were mediated by G12-dependent activation of RhoA. Thus, selective activation of ...

Tópico(s): Eicosanoids and Hypertension Pharmacology

2008 - Elsevier BV | Journal of Biological Chemistry

Artigo Revisado por pares

Chris Ritchie, Carsten Streb, Johannes Thiel, Scott G. Mitchell, Haralampos N. Miras, De‐Liang Long, Thomas Boyd, Robert D. Peacock, Thomas McGlone, Leroy Cronin,

... Chemistry, The University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland (UK), Fax: (+44) 141-330-4888 ... Chemistry, The University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland (UK), Fax: (+44) 141-330-4888 ... Chemistry, The University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland (UK), Fax: (+44) 141-330-4888 ... Chemistry, The University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland (UK), Fax: (+44) 141-330-4888 ... Chemistry, The University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland (UK), Fax: (+44) 141-330-4888 ...

Tópico(s): Chemical Synthesis and Reactions

2008 - Wiley | Angewandte Chemie

Artigo Revisado por pares

De‐Liang Long, Yu‐Fei Song, ELIZABETH WILSON, Paul Kögerler, Si‐Xuan Guo, Alan M. Bond, Justin S. J. Hargreaves, Leroy Cronin,

... Chemistry, The University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland, UK, Fax: (+44) 141-330-4888 ... Chemistry, The University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland, UK, Fax: (+44) 141-330-4888 ... Chemistry, The University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland, UK, Fax: (+44) 141-330-4888 ... Chemistry, The University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland, UK, Fax: (+44) 141-330-4888 ... Chemistry, The University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland, UK, Fax: (+44) 141-330-4888 ...

Tópico(s): Nanocluster Synthesis and Applications

2008 - Wiley | Angewandte Chemie

Revisão Revisado por pares

Thomas Worzfeld, Nina Wettschureck, Stefan Offermanns,

... G proteins: Gs, Gi/Go, Gq/G11 and G12/G13. Progress in the knowledge about the G12/G13 family has somewhat lagged behind because their ... several years, and tools to specifically interfere with G12/G13-mediated signalling were, therefore, missing. However, with the identification of G12/G13-regulated signalling pathways and the recent application ... analyses of the in vivo functions of mammalian G12/G13-mediated signalling. The human genome encodes hundreds ... G proteins: Gs, Gi/Go, Gq/G11 and G12/G13. Progress in the knowledge about the G12/ ...

Tópico(s): Cell Adhesion Molecules Research

2008 - Elsevier BV | Trends in Pharmacological Sciences

Artigo Acesso aberto Revisado por pares

Indrani Banerjee, Sasirekha Ramani, Beryl Primrose, Miren Iturriza‐Gómara, James J. Gray, David Brown, Gagandeep Kang,

... types in humans are G1‐4 and G9. G12 is a rare human rotavirus (HRV) strain first reported in the Philippines. In this study, 13 G12 strains obtained from a community‐based cohort and ... southern Indian strains had the greatest homology with G12 strains recently reported from eastern India (97–99% ... acid level) and less homology with the prototype G12 strain, L26 (89–90% identity at the nucleotide ... and the NSP4 genes revealed that the Vellore G12 strains belonged to VP6 subgroup II and NSP4 ... strains were P[6] and P[8]. A G12 type‐specific primer was designed for inclusion in ...

Tópico(s): Advanced Thermodynamic Systems and Engines

2007 - Wiley | Journal of Medical Virology

Artigo Acesso aberto Revisado por pares

Patrick Kelly, Laura N. Stemmle, John F. Madden, Timothy A. Fields, Yehia Daaka, Patrick J. Casey,

... suggested a role for the members of the G12 family of heterotrimeric G proteins (Gα12 and Gα13) ... tumor cell growth. However, few studies have examined G12 signaling in actual human cancers. In this study, we examined the role of G12 signaling in prostate cancer. We found that expression of the G12 proteins is significantly elevated in prostate cancer. Interestingly, ... RhoA family of G proteins. Furthermore, inhibition of G12 signaling by expression of the RGS domain of ... not reduce cancer cell growth. However, inhibition of G12 signaling with p115-RGS in these cell lines ...

Tópico(s): Cancer, Hypoxia, and Metabolism

2006 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

Bernhard Nieswandt, Valerie Schulte, Alexandra Zywietz, Marie‐Pierre Gratacap, Stefan Offermanns,

... alone, which in Gαq-deficient platelets couple to G12/G13 and Gi, respectively, did not induce platelet ... of Gαq-deficient platelets. Thus, the activation of G12/G13- and Gi-mediated signaling pathways is sufficient ... pathways involving Gq and Gi as well as G12/G13. Platelet activation is a complex process induced ... alone, which in Gαq-deficient platelets couple to G12/G13 and Gi, respectively, did not induce platelet ... of Gαq-deficient platelets. Thus, the activation of G12/G13- and Gi-mediated signaling pathways is sufficient ...

Tópico(s): Antiplatelet Therapy and Cardiovascular Diseases

2002 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

Abdül Waheed, Teresa L.Z. Jones,

... such as oncogenic transformation and cytoskeletal reorganization. Yet G12 and G13can act through different pathways, bind different ... on some effectors. We investigated the compartmentalization of G12 and G13 at the membrane because other G ... thereby explain some of the nonoverlapping functions of G12 and G13 and control of these potent cell ... such as oncogenic transformation and cytoskeletal reorganization. Yet G12 and G13can act through different pathways, bind different ... on some effectors. We investigated the compartmentalization of G12 and G13 at the membrane because other G ...

Tópico(s): Cell death mechanisms and regulation

2002 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

Robert T. Dorsam, Soochong Kim, Jianguo Jin, Satya P. Kunapuli,

... thrombin and thromboxane A2, the contribution of the G12/13signaling pathway to GPIIb/IIIa activation has not ... G protein pathways to investigate the contribution of G12/13 activation to platelet fibrinogen receptor activation. YFLLRNP ... at low concentrations (60 μm), selectively activates the G12/13 signaling cascade resulting in platelet shape change ... Y-27632. At this low concentration, YFLLRNP-mediated G12/13 signaling caused platelet aggregation and enhanced PAC- ... 10 nm), a thromboxane A2 mimetic, to activate G12/13 in the presence of Gi signaling. These ...

Tópico(s): Blood disorders and treatments

2002 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

Mark Anderson, Szu Shien Ng, Vanessa Marchesi, Fiona H. MacIver, Frankie Stevens, Tracy Riddell, David M. Glover, Iain Hagan, Christopher McInerny,

... Biological and Life Sciences, University of Glasgow, Glasgow, G12 8QQ UK Search for more papers by this ... Biological and Life Sciences, University of Glasgow, Glasgow, G12 8QQ UK Search for more papers by this ... Biological and Life Sciences, University of Glasgow, Glasgow, G12 8QQ UK Search for more papers by this ... Biological and Life Sciences, University of Glasgow, Glasgow, G12 8QQ UK Search for more papers by this ... Biological and Life Sciences, University of Glasgow, Glasgow, G12 8QQ UK Search for more papers by this ...

Tópico(s): Microtubule and mitosis dynamics

2002 - Springer Nature | The EMBO Journal

Artigo Acesso aberto Revisado por pares

Thomas E. Meigs, Mary Fedor‐Chaiken, Daniel D. Kaplan, Robert Brackenbury, Patrick J. Casey,

... previously demonstrated that heterotrimeric G proteins of the G12 subfamily comprised of Galpha12 and Galpha13 interact with ... cadherin-mediated cell-cell adhesion, we examined whether G12 subfamily proteins could also regulate cadherin function. The introduction of mutationally activated G12 proteins into K562 cells expressing E-cadherin blocked ... in breast cancer cells, the introduction of activated G12 proteins blocked E-cadherin function in a fast ... Aggregation mediated by a mutant cadherin that lacks G12 binding ability was not affected by activated G12 ...

Tópico(s): Cancer-related gene regulation

2002 - Elsevier BV | Journal of Biological Chemistry

Artigo Acesso aberto Revisado por pares

Marie‐Pierre Gratacap, Bernard Payrastre, Bernhard Nieswandt, Stefan Offermanns,

... αIIbβ3activation. U46619, which leads to the activation of G12/G13 and Gq did not induce Rac activation ... inhibited the U46619-induced activation of Gq and G12/G13 as well as of Rac and Rho. ... are differentially regulated through heterotrimeric G-proteins. The G12/G13-mediated Rho activation is involved in the ... αIIbβ3activation. U46619, which leads to the activation of G12/G13 and Gq did not induce Rac activation ... inhibited the U46619-induced activation of Gq and G12/G13 as well as of Rac and Rho. ... are differentially regulated through heterotrimeric G-proteins. The G12/G13-mediated Rho activation is involved in the ...

Tópico(s): Cell Adhesion Molecules Research

2001 - Elsevier BV | Journal of Biological Chemistry