Brian Bae, Elizabeth A. Olmsted‐Davis, Daniel R. Brown, Elizabeth A. Campbell, Sivaramesh Wigneshweraraj, Seth A. Darst,
... RNA polymerase (RNAP), the product of gene 2 (Gp2). We determined a series of X-ray crystal structures of E. coli RNAP holoenzyme with or without Gp2. The results define the structure and location of ...
Tópico(s): Bacterial Genetics and Biotechnology
2013 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Snehal G. Patel, David B. McWilliams, Christine T. Fischette, Jaye Thompson, Mira A. Patel, F. Joseph Daugherty,
... It was previously reported that completion of the GP2+GM-CSF Primary Immunization Series (PIS) reduced recurrence rates to 0% over a 5 year follow-up period in HER2 3+ patients, who received a standard course of trastuzumab after surgery. Methods: Each enrolled and consented patient was randomly scheduled to receive a total of 6 GP2+GM-CSF (500 mcg GP2: 125 mcg GM- ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2021 - Lippincott Williams & Wilkins | Journal of Clinical Oncology
Elizabeth D. Dolci, George E. Palade,
... 45-60-min chase. [3H]Palmitate labeled a series of splenic cell proteins, including gp2, gp3, and gp3pr. Chemical analyses indicated that the ...
Tópico(s): Pancreatic function and diabetes
1985 - Elsevier BV | Journal of Biological Chemistry
Lawrence A. Stern, Ian Schrack, Sadie Johnson, Aakash Deshpande, Nathaniel R. Bennett, Lauren A. Harasymiw, Melissa K. Gardner, Benjamin J. Hackel,
... towards ligands for genuine cellular target. Using a series of fibronectin domain, affibody, and Gp2 ligands and human cell lines expressing a range ...
Tópico(s): Viral Infectious Diseases and Gene Expression in Insects
2016 - Wiley | Biotechnology and Bioengineering
Snehal S. Patel, David B. McWilliams, Marisa Patel, Christine T. Fischette, Jaye Thompson, F. Joseph Daugherty,
... total of 6 GP2+GM-CSF (500 mcg GP2: 125 mcg GM-CSF) intradermal injections every 3-4 weeks as part of the Primary Immunization Series (PIS) for the first 6 months and 4 GP2+GM-CSF booster intradermal injections every 6 months ...
Tópico(s): Cancer Genomics and Diagnostics
2021 - American Association for Cancer Research | Cancer Research
... with established PD consortia; however, in some instances GP2 will support the collection of additional patients, particularly from underserved communities. To ensure a functional and efficient structure, we created a series of working groups and hubs that center on achieving specific aims and priorities within GP2. Although these groups have clear aims and deliverables, ... the pathogenesis of disease. ASAP has supported a series of ambitious projects within the Collaborative Research Network (https://parkinsonsroadmap.org/research-network/) with a major focus on work to determine the biology of PD-associated genetics. GP2 will be a foundational resource for these studies. ...
Tópico(s): Autism Spectrum Disorder Research
2021 - Wiley | Movement Disorders
Xin Sui, Beat Frey, Libin Yang, Yingnan Liu, Rongtao Zhang, Hongwei Ni, Mai‐He Li,
... subgroups of Acidobacteria accounted for 31% (Gp1), 5% (Gp2), 12% (Gp3), 2% (Gp4), 5% (Gp6), and 2% (Gp7) in soils within eight successional series. The compositions of soil Acidobacteria in wetland stages ...
Tópico(s): Biogeochemical Cycling of Nutrients in Aquatic Ecosystems
2022 - Frontiers Media | Frontiers in Microbiology
Erika J Schneble, Sonia A. Perez, James L. Murray, John Berry, Alfred F. Trappey, Timothy J. Vreeland, Diane F. Hale, Julia M. Greene, Guy T. Clifton, Alexandros Ardavanis, Jennifer K. Litton, Sathibalan Ponniah, Nathan M. Shumway, Michael Papamichail, George E. Peoples, Elizabeth A. Mittendorf,
... include trastuzumab where appropriate) were randomized to receive GP2+GM-CSF (VG) or GM-CSF (CG) alone. Pts received 6 monthly inoculations (primary vaccine series = PVS) followed by 4 boosters administered every 6 ...
Tópico(s): Cancer Genomics and Diagnostics
2014 - Lippincott Williams & Wilkins | Journal of Clinical Oncology
Guy T. Clifton, Jarrod P. Holmes, Sonia A. Perez, D. Lorentz, K. Georgakopoulou, Linda C. Benavides, Jeremy D. Gates, Maj Elizabeth A. Mittendorf, Alexandros Ardavanis, Angelos D. Gritzapis, Sathibalan Ponniah, Michael Papamichail, George E. Peoples,
... toxicities in either arm. Median DTH reaction to GP2 increased significantly from pre-vaccination level after completion of the primary series (post-vaccination) in the PG group (1.0± ... without SI post-vaccination (p=0.38). The % GP2-specific CD8+ lymphocytes significantly increased from baseline at 6 months after completion of the primary series in the PG (0.65±0.15 to ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2009 - American Association for Cancer Research | Cancer Research
Christian Raschner, Hans-Peter Platzer, Carson Patterson,
... experienced drivers consisted of five Formula One, two GP2 and two Formula 3 drivers, and the nine junior drivers drove in the Formula Master, Koenig, BMW and Renault series. The following fitness parameters were tested: multiple reactions, ...
Tópico(s): Winter Sports Injuries and Performance
2012 - Taylor & Francis | Journal of Sports Sciences
Elizabeth A. Mittendorf, Alexandros Ardavanis, Jennifer K. Litton, Nathan M. Shumway, Diane F. Hale, James L. Murray, Sonia A. Perez, Sathibalan Ponniah, Constantin N. Baxevanis, Michael Papamichail, George E. Peoples,
... benefit to vaccination, this trial confirmed that the GP2 vaccine is safe and suggests that vaccination may have clinical activity, particularly in patients with HER2 overexpression who received the full vaccine series (ie per-treatment group).
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2016 - Impact Journals LLC | Oncotarget
Yifeng Jiang, Tong Wu, Lingxue Yu, Liwei Li, Fei Gao, Guoxin Li, Changlong Liu, Pengfei Chen, Qi Shen, Yujiao Zhang, Yanjun Zhou, Guangzhi Tong,
... vitro and cytokine response. Among 9 PRRSV attenuated series, consistent amino acid changes during PRRSV attenuation were found in NSP4, NSP9, GP2, E, GP3 and GP4. Our study provides a ...
Tópico(s): Virus-based gene therapy research
2021 - Multidisciplinary Digital Publishing Institute | Viruses
Erika J Schneble, John Berry, Alfred F. Trappey, Timothy J. Vreeland, Diane F. Hale, Alan K. Sears, Guy T. Clifton, Sathibalan Ponniah, Elizabeth A. Mittendorf, George E. Peoples,
... points although both groups sustained an increase in GP2-CTLs throughout the booster series. Conclusions: The GP2 peptide vaccine and boosters are safe with toxicities ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2014 - American Association for Cancer Research | Cancer Research
Juanelle Du Plessis, Ruben Cloete, Lynn Burchell, Paramita Sarkar, Robin M. Warren, Alan Christoffels, Sivaramesh Wigneshweraraj, Samantha L. Sampson,
... assay and in vitro transcription assay revealed that Gp2 binds to mycobacterial RNAP and inhibits transcription; however to a much lesser degree than in E. coli. To further understand the molecular basis of these results, a series of in silico techniques were used to assess the interaction between mycobacterial RNAP and Gp2, providing valuable insight into the characteristics of this ...
Tópico(s): Bacterial Genetics and Biotechnology
2017 - Elsevier BV | Tuberculosis
Guy T. Clifton, Holmes Jarrod, Linda C. Benavides, Jeremy D. Gates, Suzanne Mccall, Stanley Rivera, Elizabeth A. Mittendorf, Alexander Stojadinovic, Gerald A. Merrill, Sathibalan Ponniah, George E. Peoples,
... no detectable CTCs after completion of the vaccine series. The one GP2 patient with CTCs after completing the primary vaccine inoculations had a clinically detected cancer recurrence shortly after completion of the vaccine series. Conclusions: CTCs may be an early marker for therapeutic effect of cancer vaccines in the adjuvant setting. Initial results from our clinical trial show that patients treated with the GP2 peptide vaccine are more likely to have a ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2009 - American Association for Cancer Research | Cancer Research
Francois Trappey, John Berry, Timothy J. Vreeland, Diane F. Hale, Alan K. Sears, Sathibalan Ponniah, Sonia A. Perez, Guy T. Clifton, Michael Papamichail, George E. Peoples, Elizabeth A. Mittendorf,
... therapy. HLA-A2+ pts were randomized to receive GP2 + GM-CSF (VG) or GM-CSF alone (CG). HLA A2- controls from a parallel arm of the study were also eligible for evaluation, the extended CG (ECG). Pts receive 6 monthly intradermal inoculations (R0-R6) during the primary vaccine series followed by four boosters every 6 mos. Immune ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2013 - Lippincott Williams & Wilkins | Journal of Clinical Oncology
Yue Hu, Guiling Wu, Qinrui Jia, Baozhong Zhang, Wencheng Sun, Ruixue Sa, Siyu Zhang, Weifan Cai, JARHEN JARHEN, Ran Duo-liang, Jianhua Liu,
... molecular marker for DIVA. In this study, a series of EqAHV1 mutants with deletion of gp2 along with other virulence genes (TK, UL24/TK, ...
Tópico(s): Virus-based gene therapy research
2024 - Frontiers Media | Frontiers in Immunology
... patients were not boosted after their primary vaccine series,” he said. GP2 and AE37 are currently undergoing Phase II testing ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2009 - Wolters Kluwer | Oncology Times
Edel M. Hyland, Lisa F. Rezende, Charles C. Richardson,
... 5 protein. Similar results have been seen with gp2.5-Δ21C, also a monomer in solution, where ssDNA binding was analyzed by surface plasmon resonance. 4J. M. Stattel and C. C. Richardson, unpublished data. The electrophoretic mobility shift assay employed in this study provided an insight into the mode by which wild-type gene 2.5 protein binds ssDNA. Over a protein concentration series from 80 to 10,600 nm, upon binding ...
Tópico(s): DNA Repair Mechanisms
2003 - Elsevier BV | Journal of Biological Chemistry
... on a host protein, integration host factor. A series of mutations in gene 1 (her mutations), which allow integration host factor-independent DNA packaging by 21, were found to be missense changes that affect predicted alpha-helixes in gp1. gp2, the large terminase subunit, is predicted to contain ...
Tópico(s): Bacterial Genetics and Biotechnology
1993 - American Society for Microbiology | Journal of Bacteriology
... 1991) used the MLE method to fit the GP2 distribution to represent the Dutch POT rainfall series, and used an empirical correction formula to reduce bias of the scale and shape parameter estimates. Davison and Smith (1990) used MLE, PWM, and a graphical method to estimate the GP2 distribution parameters. Guo and Singh (1992) and Singh ...
Tópico(s): Statistical Distribution Estimation and Applications
1998 - Springer Nature (Netherlands) | Water science and technology library
Yu Zhang, Mobing Zhuang, Pablo Campo, Ruta Suresh Deshpande, Devi Sundaravadivelu, Robyn N. Conmy, Jorge W. Santo Domingo,
... at 25 °C for 48 days using sterile GP2 artificial seawater as the media. The time series concentration of DOSS, the primary surfactant in the ...
Tópico(s): Toxic Organic Pollutants Impact
2017 - Atlantis Press | International Oil Spill Conference Proceedings
Snehal S. Patel, David B. McWilliams, Mira S. Patel, Christine T. Fischette, Jaye Thompson, F Joseph Daugherty,
Tópico(s): Immunotherapy and Immune Responses
2021 - SAGE Publishing | Clinical Trials
Amitabh V. Nimonkar, Paul E. Boehmer,
... are important for efficient RDR, we performed a series of experiments wherein each of the HSV-1 replication components was replaced by its counterpart from a variety of systems. First, we substituted ICP8 with SSBs from other systems, namely hRP-A, E-SSB, T4 gp32, and T7 gp2.5. Fig. 4 depicts the results of this ...
Tópico(s): DNA Repair Mechanisms
2004 - Elsevier BV | Journal of Biological Chemistry
Tommy A. Brown, Elizabeth A. Mittendorf, Diane F. Hale, John W. Myers, Kaitlin M. Peace, Doreen O. Jackson, Julia M. Greene, Timothy J. Vreeland, Guy T. Clifton, Alexandros Ardavanis, Jennifer K. Litton, Nathan M. Shumway, James T. Symanowski, James L. Murray, Sathibalan Ponniah, Eleftheria A. Anastasopoulou, Nikolaos Pistamaltzian, Constantin N. Baxevanis, Sonia A. Perez, Michael Papamichail, George E. Peoples,
... control (CG) were given as the primary vaccine series with 4 boosters at 6-month intervals. Demographic, safety, immunologic, and DFS data were evaluated.456 patients were enrolled; 154 patients in the VG and 147 in CG for AE37, 89 patients in the VG and 91 in CG for GP2. The AE37 arm had no difference in DFS ...
Tópico(s): HER2/EGFR in Cancer Research
2020 - Springer Science+Business Media | Breast Cancer Research and Treatment
JS Berry, AF Trappey, TJ Vreeland, EJ Schneble, GT Clifton, DF Hale, AK Sears, Sathibalan Ponniah, NM Shumway, EA Mittendorf, GE Peoples,
... with separate intradermal inoculations of AE37, AE36, and GP2 antigens, measured both pre-vaccination (R0) and after the vaccine series (R6) via the sensitive ballpoint pen method, and ...
Tópico(s): vaccines and immunoinformatics approaches
2013 - American Association for Cancer Research | Cancer Research
Christos Liaskos, Vassiliki Spyrou, Labrini V. Athanasiou, Timoklia Orfanidou, Αthanasios Mavropoulos, Eirini I. Rigopoulou, G.S. Amiridis, Yehuda Shoenfeld, Charalambos Billinis, Dimitrios P. Bogdanos,
... 1/160. Methodological flaws were prevented by a series of tests. Control sera from anti-CUZD1 positive CrD samples have shown anti-CUZD1 antibody reactivity at various concentrations. Antibody reactivity to GP2-expressing HEK293 cells has confirmed the reactivity to ...
Tópico(s): Inflammatory Bowel Disease
2015 - Elsevier BV | Clinics and Research in Hepatology and Gastroenterology
Alan K. Sears, Guy T. Clifton, Mark G. Carmichael, David C. Van Echo, Jarrod P. Holmes, Athina Zacharia, Yusuf Jama, Mohamed Mursal, Anna Chiplis, Elizabeth A. Mittendorf, George E. Peoples, Sathibalan Ponniah,
... proliferation for AE37, HLA-A2:immunoglobulin dimer for GP2) and in vivo (delayed-type hypersensitivity (DTH)) immune responses were measured before (R0), mid-series (R3), upon completion (R6), and at six (RC6) ... twelve (RC12) months after completion of the vaccine series. RESULTS: We have enrolled 247 patients (AE37=171, 87 vaccine and 84 control) (GP2=76, 41 vaccine and 35 control). Proliferative responses ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2011 - American Association for Cancer Research | Cancer Research
Paula Saffie Awad, Katja Lohmann, Yasmin Hirmas, Frauke Hinrichs, Mirja Thomsen, Marcelo A. Kauffman, Theresa Lüth, Joanne Trinh, Ana Westenberger, Pedro Chaná‐Cuevas, Christine Klein,
... supported by the Global Parkinson's Genetics Program (GP2). GP2 is funded by the Aligning Science Against Parkinson' ... Fox Foundation for Parkison's Research (https://www.gp2.org). For a complete list of GP2 members see https://gp2.org. We thank the authorities of Fresia, especially ... María. Katja Lohmann: Grants: German Research Foundation, MJFF (GP2), Damp foundation, and Parkinson's Foundation; Employment: University ...
Tópico(s): Endoplasmic Reticulum Stress and Disease
2023 - Wiley | Movement Disorders
Hasan A. Sohail, Aidan Coffey, Krystyna Debrowska, Irmtraud M. Meyer, Mathias Middelboe, Muhammad Sohail, Martha R. J. Clokie,
... model phage, T7, two small 7 kDa proteins, Gp2 and Gp5.7, collaborate to inhibit the E. ... model system and showed that intracellular induction of Gp2 (but not Gp5.7) reduces bacterial load in BMM, whereas induction of both Gp2 and Gp5.7 reduces formation of Salmonella persisters, ... the first clinical study to report a patient series that demonstrated safety and feasibility of intravenously administered ...
Tópico(s): Genetics, Bioinformatics, and Biomedical Research
2020 - Mary Ann Liebert, Inc. | PHAGE