Rui Pedro Galão, Suzanne Pickering, Rachel Curnock, Stuart J. D. Neil,
Tetherin (BST2/CD317) restricts the release of enveloped viral particles from infected cells. Coupled to this virion retention, hominid tetherins induce proinflammatory gene expression via activating NF-κB. We investigated the events initiating this tetherin-induced signaling and show that physical retention of retroviral particles induces the phosphorylation of conserved tyrosine residues in the cytoplasmic tails of tetherin dimers. This phosphorylation induces the recruitment of spleen tyrosine ...
Tópico(s): Herpesvirus Infections and Treatments
2014 - Cell Press | Cell Host & Microbe
Suzanne Pickering, Gilberto Betancor, Rui Pedro Galão, Blair Merrick, Adrian W. Signell, Harry Wilson, Mark Tan Kia Ik, Jeffrey Seow, Carl Graham, Sam Acors, Neophytos Kouphou, Kathryn J. A. Steel, Oliver Hemmings, Amita Patel, Gaia Nebbia, Sam Douthwaite, Lorcan O’Connell, Jakub Lupták, Laura E. McCoy, Philip J. M. Brouwer, Marit J. van Gils, Rogier W. Sanders, Rocio Martinez Nunez, Karen Bisnauthsing, Geraldine O’Hara, Eithne MacMahon, Rahul Batra, Michael H. Malim, Stuart J. D. Neil, Katie J. Doores, Jonathan D. Edgeworth,
There is a clear requirement for an accurate SARS-CoV-2 antibody test, both as a complement to existing diagnostic capabilities and for determining community seroprevalence. We therefore evaluated the performance of a variety of antibody testing technologies and their potential use as diagnostic tools. Highly specific in-house ELISAs were developed for the detection of anti-spike (S), -receptor binding domain (RBD) and -nucleocapsid (N) antibodies and used for the cross-comparison of ten commercial serological ...
Tópico(s): COVID-19 Clinical Research Studies
2020 - Public Library of Science | PLoS Pathogens
... 1511 from Java past Bali, Lombok, Sumbawa, Solor, Galao and Malua (Alor). Pigafetta's account and chart ...
Tópico(s): Asian Studies and History
1982 - Brill | Bijdragen tot de taal- land- en volkenkunde / Journal of the Humanities and Social Sciences of Southeast Asia
... lesser bushbaby group, Galago senegalensis, Galago moholi and Galao zanzibaricus. An investigation of the ontogenetic development of ...
Tópico(s): Plant and animal studies
2009 - Wiley | Journal of Zoological Systematics & Evolutionary Research
Nicoletta Scheller, Leonardo Mina, Rui Pedro Galão, Ashwin Chari, Mireia Giménez‐Barcons, Amine Noueiry, Utz Fischer, Andreas Meyerhans, Juana Díez,
Inevitably, viruses depend on host factors for their multiplication. Here, we show that hepatitis C virus (HCV) RNA translation and replication depends on Rck/p54, LSm1, and PatL1, which regulate the fate of cellular mRNAs from translation to degradation in the 5'-3'-deadenylation-dependent mRNA decay pathway. The requirement of these proteins for efficient HCV RNA translation was linked to the 5' and 3' untranslated regions (UTRs) of the viral genome. Furthermore, LSm1-7 complexes specifically interacted ...
Tópico(s): Hepatitis B Virus Studies
2009 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Shiela M. Strauss, Alla J. Wheeler, Stefanie L. Russell, Anya Brodsky, Robert M. Davidson, Rima Gluzman, Li Lin, Roberto Galao Malo, Bram Salis, Robert Schoor, Krassimira Tzvetkova,
Background: This study examined conditions under which gingival crevicular blood (GCB) could be used to obtain a useful glucose reading to screen for undiagnosed diabetes during routine dental visits. Methods: GCB and capillary finger‐stick blood (CFB) glucose readings obtained with a glucometer were compared for 46 patients recruited from an urban university dental clinic. Study participants were divided into two groups based on probing depth or bleeding on probing (BOP) at the site of collection ...
Tópico(s): Diabetic Foot Ulcer Assessment and Management
2009 - Wiley | Journal of Periodontology
António Paulo Gouveia de Almeida, Rui Pedro Galão, Carla A. Sousa, María Teresa Novo, Ricardo Parreira, João Pinto, J. Piedade, Aida Esteves,
Circulation of West Nile virus in Portugal was demonstrated by serological surveys, and the virus was isolated in 1969 from Anopheles maculipennis s.l. A survey of the whole country was carried out (2001-2004) to assess the abundance of mosquito species and to screen them for arbovirus infection. A total of 770 collections yielded 32460 mosquitoes of 15 species. The regions with the highest abundance of mosquitoes were the coastal and estuarine districts of Santarém, Setúbal and Faro. Culex pipiens ...
Tópico(s): Malaria Research and Control
2008 - Oxford University Press | Transactions of the Royal Society of Tropical Medicine and Hygiene
Rui Pedro Galão, Ashwin Chari, Isabel Alves‐Rodrigues, Daniela Lobão, Antonio Más, Christian Kambach, Utz Fischer, Juana Díez,
LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3'-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that ...
Tópico(s): Plant and Fungal Interactions Research
2010 - Cold Spring Harbor Laboratory Press | RNA
Produção Nacional
Revisado por pares
Carmen Luı́sa Barbosa Guedes, Daniele Cristina Adão, Talita Pedroso Quessada, Dionísio Borsato, Olívio Fernandes Galão, Eduardo Di Mauro, Juan Miguel Mesa Pérez, José Dílcio Rocha,
AS GASOLINE ADDICTIVE.A biofuel was prepared from acid aqueous fraction (pH = 2) of bio-oil produced by fast pyrolysis (Bioware Technology) of lignocellulosic biomass (sugar cane residue) and tested in blends (2, 5, 10 e 20% v/v) with gasoline type C (common) marketed in Brazil.The specification tests made in the Refinery President Getúlio Vargas (PETROBRAS) showed increasing in the octane number (MON) and antiknock index (AKI) with reduction in the residue generation during the combustion.The physicochemical ...
Tópico(s): Biodiesel Production and Applications
2010 - Brazilian Chemical Society | Química Nova
Harriet C. T. Groom, Melvyn W. Yap, Rui Pedro Galão, Stuart J. D. Neil, Kate N. Bishop,
Xenotropic murine leukemia virus-related virus (XMRV) is a recently discovered gammaretrovirus that has been linked to prostate cancer and chronic fatigue syndrome. This virus is therefore an important potential human pathogen and, as such, it is essential to understand its host cell tropism. Intriguingly, infectious virus has been recovered from patient-derived peripheral blood mononuclear cells. These cells express several antiviral restriction factors that are capable of inhibiting the replication ...
Tópico(s): Herpesvirus Infections and Treatments
2010 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Nicoletta Scheller, Patricia Resa‐Infante, Susana de la Luna, Rui Pedro Galão, Mario Albrecht, Lars Kaestner, Peter Lipp, Thomas Lengauer, Andreas Meyerhans, Juana Díez,
In yeast, the activators of mRNA decapping, Pat1, Lsm1 and Dhh1, accumulate in processing bodies (P bodies) together with other proteins of the 5′-3′-deadenylation-dependent mRNA decay pathway. The Pat1 protein is of particular interest because it functions in the opposing processes of mRNA translation and mRNA degradation, thus suggesting an important regulatory role. In contrast to other components of this mRNA decay pathway, the human homolog of the yeast Pat1 protein was unknown. Here we describe ...
Tópico(s): RNA and protein synthesis mechanisms
2007 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Rui Pedro Galão, Nicoletta Scheller, Isabel Alves‐Rodrigues, Tanja Breinig, Andreas Meyerhans, Juana Díez,
The yeast Saccharomyces cerevisiae is a well-established model system for understanding fundamental cellular processes relevant to higher eukaryotic organisms. Less known is its value for virus research, an area in which Saccharomyces cerevisiae has proven to be very fruitful as well. The present review will discuss the main achievements of yeast-based studies in basic and applied virus research. These include the analysis of the function of individual proteins from important pathogenic viruses, ...
Tópico(s): Plant Disease Resistance and Genetics
2007 - BioMed Central | Microbial Cell Factories
V.T. Giner, F. Javier Baeza, Salvador Ivorra, E. Zornoza, Ó. Galao,
Fiber reinforced concretes (FRC) are technologically important due to their combination of good structural properties, durability and multifunctional properties. The behavior of a structure under dynamic actions is determined by its dynamic mechanical properties and its total overall damping. In this work the influence of the steel fiber (SF) and carbon fiber (CF) additions on the mechanical properties of concrete containing silica fume has been studied, focusing on its passive material damping ability ( ...
Tópico(s): Structural Behavior of Reinforced Concrete
2011 - Elsevier BV | Materials & Design (1980-2015)
Isabel Alves‐Rodrigues, Rui Pedro Galão, Andreas Meyerhans, Juana Díez,
Understanding the fundamental steps of virus life cycles including virus–host interactions is essential for the design of effective antiviral strategies. Such understanding has been deferred by the complexity of higher eukaryotic host organisms. To circumvent experimental difficulties associated with this, systems were developed to replicate viruses in the yeast Saccharomyces cerevisiae. The systems include viruses with RNA and DNA genomes that infect plants, animals and humans. By using the powerful ...
Tópico(s): Plant Disease Resistance and Genetics
2006 - Elsevier BV | Virus Research
P. Garcés, E. Zornoza, E. G. Alcocel, Ó. Galao, L.G. Andión,
The changes in mechanical properties of Calcium Aluminate Cement (CAC) mortars due to the addition of carbon fibers (CFs) to the mix have been studied. Compressive and flexural strengths have been determined in relation to the amount of CF added to the mix, water/cement ratio (w/c), curing time and porosity. The relationship between the values of ultrasonic pulse velocity (UPV) and compressive strengths for CAC mortars have also been obtained. Additionally the corrosion level of reinforcing steel bars ...
Tópico(s): Concrete and Cement Materials Research
2012 - Elsevier BV | Construction and Building Materials
Emily A. Bruce, Truus E. M. Abbink, Helen Wise, Ruth Rollason, Rui Pedro Galão, George Banting, Stuart J. D. Neil, Paul Digard,
The cellular protein tetherin is thought to act as a 'leash' that anchors many enveloped viruses to the plasma membrane and prevents their release. We found that replication of multiple strains of influenza A virus was generally insensitive to alteration of tetherin levels, as assessed by output titre or scanning electron microscopy of cell-associated virions. This included human, swine, avian and equine isolates, strains that form filamentous or spherical particles and viruses that lack the M2 or ...
Tópico(s): Virology and Viral Diseases
2012 - Microbiology Society | Journal of General Virology
Beatriz del Moral Delgado, Ó. Galao, C. Antón, Montserrat Climent, P. Garcés,
RESUMENEn la aplicación de la técnica de extracción electroquímica de cloruros (EEC), tradicionalmente se ha venido empleando como ánodo externo una malla de Ti-RuO 2 .En este artículo se aportan los resultados de investigaciones basadas en la utilización de ánodos formados por pasta de cemento conductora con adición de nanofibras de carbono (NFC) y su aplicación en EEC.Las experiencias se desarrollaron en probetas de hormigón contaminado previamente con cloruro.Las eficiencias alcanzadas se compararon ...
Tópico(s): Electrochemical Analysis and Applications
2012 - Spanish National Research Council | Materiales de Construcción
Ó. Galao, E. Zornoza, F. Javier Baeza, Ana M. Bernabéu, P. Garcés,
Efecto de la adición de nanofibras de carbono en las propiedades mecánicas y de durabilidad de materiales cementantes Effect of carbon nanofiber addition in the mechanical properties and durability of cementitious materials
Tópico(s): Concrete and Cement Materials Research
2012 - Spanish National Research Council | Materiales de Construcción
Rui Pedro Galão, Anna Le Tortorec, Suzanne Pickering, Tonya Kueck, Stuart J. D. Neil,
Antiviral proteins that recognize pathogen-specific or aberrantly located molecular motifs are perfectly positioned to act as pattern-recognition receptors and signal to the immune system. Here we investigated whether the interferon-induced viral restriction factor tetherin (CD317/BST2), which is known to inhibit HIV-1 particle release by physically tethering virions to the cell surface, has such a signaling role. We find that upon restriction of Vpu-defective HIV-1, tetherin acts as a virus sensor ...
Tópico(s): Immune Response and Inflammation
2012 - Cell Press | Cell Host & Microbe
Aida Esteves, António Paulo Gouveia de Almeida, Rui Pedro Galão, Ricardo Parreira, J. Piedade, João Rodrigues, Carla A. Sousa, María Teresa Novo,
West Nile virus (WNV) genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region, amplified from Culex pipiens s.l. and Culex univittatus unfed females, demonstrated a close relationship with WNV strains circulating in the Mediterranean basin (Italy, 1998; ...
Tópico(s): Malaria Research and Control
2005 - Mary Ann Liebert, Inc. | Vector-Borne and Zoonotic Diseases
Produção Nacional
Revisado por pares
Adriani Oliveira Galão, Lívia Hartmann de Souza, Bartira Ercília Pinheiro da Costa, Rosane Scheibe, Carlos Eduardo Poli‐de‐Figueiredo,
Objective The purpose of this study was to evaluate the angiotensin-converting enzyme gene polymorphism in pregnant women with and without preeclampsia. Study design Preeclampsia was defined as hypertension and pathologic proteinuria in pregnant women after gestational week 20. Genomic DNA was isolated from leukocytes. The insertion-deletion polymorphism in intron 16 of the angiotensin-converting enzyme gene was detected in DNA samples with the use of the polymerase chain reaction. Chi-squared and ...
Tópico(s): Renin-Angiotensin System Studies
2004 - Elsevier BV | American Journal of Obstetrics and Gynecology
Produção Nacional
Ludimila Rodrigues Reis, Paula Días Bevilacqua, Rose Ferraz Carmo,
... de quatro marcas de água mineral envasada em galão de 20 L. A qualidade dessas águas foi ...
Tópico(s): Water Quality and Pollution Assessment
2014 - Instituto de Estudos em Saúde Coletiva da Universidade Federal do Rio de Janeiro | Cadernos Saúde Coletiva
F. Javier Baeza, J. Payá, Ó. Galao, José Miguel Saval, P. Garcés,
Binary and ternary combinations of sewage sludge ash (SSA) with marble dust (MD), fly ash (FA) and rice husk ash (RHA) as replacement in Portland cement pastes, were assessed. Several tests were carried out at different curing ages: thermogravimetry, density, water absorption, ultrasonic pulse velocity and mechanical strengths. Pozzolanic effects of the mineral admixtures, densities similar to control sample and improved absorptions when combining waste materials were identified. In general, the compressive ...
Tópico(s): Recycled Aggregate Concrete Performance
2014 - Elsevier BV | Construction and Building Materials
María Del Carmen Camacho, Ó. Galao, F. Javier Baeza, E. Zornoza, P. Garcés,
In the present paper, changes in mechanical properties of Portland cement-based mortars due to the addition of carbon nanotubes (CNT) and corrosion of embedded steel rebars in CNT cement pastes are reported. Bending strength, compression strength, porosity and density of mortars were determined and related to the CNT dosages. CNT cement paste specimens were exposed to carbonation and chloride attacks, and results on steel corrosion rate tests were related to CNT dosages. The increase in CNT content ...
Tópico(s): Concrete and Cement Materials Research
2014 - Multidisciplinary Digital Publishing Institute | Materials
Produção Nacional
Revisado por pares
Olívio Fernandes Galão, M. C. Carrão-Panizzi, J. M. G. Mandarino, Oscar Oliveira Santos, Swami A. Maruyama, Luana Caroline de Figueiredo, Elton G. Bonafé, Jesuí Vergílio Visentainer,
The aim of this study was to compare fatty acid profiles of seed samples from twenty different soybean (Glycine max (L.) Merrill) genotypes (14 non-transgenic and six transgenic Roundup-tolerant) grown at two different locations, both in the Parana state, a southern region of Brazil. A total of eleven fatty acids were detected and quantified, among them the most expressive ones were oleic, linoleic, linolenic and palmitic acids. The total unsaturated fatty acid amount was higher than 82%. An increase ...
Tópico(s): Lipid metabolism and biosynthesis
2014 - Elsevier BV | Food Research International
Juan F. Arias, David T. Evans,
... virus release from infected cells. In this issue, Galão et al. (2014) provide important insights into the ...
Tópico(s): RNA regulation and disease
2014 - Cell Press | Cell Host & Microbe
Ó. Galao, F. Javier Baeza, E. Zornoza, P. Garcés,
The viability of carbon nanofiber (CNF) composites in cement matrices as a self-heating material is reported in this paper.This functional application would allow the use of CNF cement composites as a heating element in buildings, or for deicing pavements of civil engineering transport infrastructures, such as highways or airport runways.Cement pastes with the addition of different CNF dosages (from 0 to 5% by cement mass) have been prepared.Afterwards, tests were run at different fixed voltages (50, ...
Tópico(s): Advanced Energy Technologies and Civil Engineering Innovations
2014 - Spanish National Research Council | Materiales de Construcción
Joaquı́n Gomis, Ó. Galao, Vicente Gomis, E. Zornoza, P. Garcés,
This research studies the self-heating produced by the application of an electric current to conductive cement pastes with carbonaceous materials. The main parameters studied were: type and percentage of carbonaceous materials, effect of moisture, electrical resistance, power consumption, maximum temperature reached and its evolution and ice melting kinetics are the main parameters studied. A mathematical model is also proposed, which predicts that the degree of heating is adjustable with the applied ...
Tópico(s): Concrete Corrosion and Durability
2014 - Elsevier BV | Construction and Building Materials
F. Javier Baeza, Ó. Galao, E. Zornoza, P. Garcés,
In this research, strain-sensing and damage-sensing functional properties of cement composites have been studied on a conventional reinforced concrete (RC) beam. Carbon nanofiber (CNFCC) and fiber (CFCC) cement composites were used as sensors on a 4 m long RC beam. Different casting conditions (in situ or attached), service location (under tension or compression) and electrical contacts (embedded or superficial) were compared. Both CNFCC and CFCC were suitable as strain sensors in reversible (elastic) ...
Tópico(s): Advanced Energy Technologies and Civil Engineering Innovations
2013 - Multidisciplinary Digital Publishing Institute | Materials
Caroline Goujon, Torsten Schaller, Rui Pedro Galão, Sarah M. Amie, Baek Kim, Kevin C. Olivieri, Stuart J. D. Neil, Michael H. Malim,
Abstract Background Type I interferon (IFN) treatment of some cells, including dendritic cells, macrophages and monocytic THP-1 cells, restricts HIV-1 infection and prevents viral cDNA accumulation. Sterile alpha motif and HD domain protein 1 (SAMHD1), a dGTP-regulated deoxynucleotide triphosphohydrolase, reduces HIV-1 infectivity in myeloid cells, likely by limiting dNTPs available for reverse transcription, and has been described as IFNα-inducible. Myeloid cell infection by HIV-1 is enhanced by ...
Tópico(s): Immune Response and Inflammation
2013 - BioMed Central | Retrovirology