Guangxin Ren, Ning Gan, Yan Song, Jingming Ning, Zhengzhu Zhang,
The feature of external shape in tea is a vital quality index that determines the rank quality of tea. The potential of a lab-made computer vision system (CVS) coupled with morphological features and chemometric tools is investigated for evaluating Congou black tea quality. First, Raw images of 700 tea samples from seven different quality grades are acquired using the CVS. The original images collected are processed by graying, binarization, and median de-noising. Then, six morphological parameters ( ...
Tópico(s): Sensory Analysis and Statistical Methods
2020 - Elsevier BV | Microchemical Journal
Wenbo Gan, Zhenli Xu, Yaowei Li, Wenchao Bi, Luoyao Chu, Qianyu Qi, Yitao Yang, Peiqing Zhang, Ning Gan, Shixun Dai, Tiefeng Xu,
The rapid and reliable detection of bacteria plays an important role in clinical and veterinary practice. A stable, label free, compact, and sensitive long-period fiber grating (LPFG) sensor based on egg yolk antibody (IgY) was proposed for the detection of Staphylococcus aureus (S. aureus). LPFG was fabricated with laser writing technology, and specific IgY was further immobilized on the grating region and then combined with the corresponding bacteria. S. aureus was detected by tracking the change ...
Tópico(s): Acoustic Wave Resonator Technologies
2021 - Elsevier BV | Biosensors and Bioelectronics
Bingqian Li, Shengrong Yu, Rong Feng, Zhiling Qian, Kangdi He, Guojiang Mao, Yuting Cao, Keqi Tang, Ning Gan, Yong‐Xiang Wu,
Bioimaging is widely used in various fields of modern medicine. Fluorescence imaging has the advantages of high sensitivity, high selectivity, noninvasiveness, in situ imaging, and so on. However, one-photon (OP) fluorescence imaging has problems, such as low tissue penetration depth and low spatiotemporal resolution. These disadvantages can be solved by two-photon (TP) fluorescence imaging. However, TP imaging still uses fluorescence intensity as a signal. The complexity of organisms will inevitably ...
Tópico(s): Advanced Nanomaterials in Catalysis
2023 - American Chemical Society | Analytical Chemistry
Shu Xiao, Cong Cao, Tinghong Ming, Yuting Cao, Zhenzhong Yu, Ning Gan,
Simultaneous detection of live and dead bacteria is a huge challenge for food safety. To solve this issue, an all-in-one biosensor for bacteria was developed using the phage-apoferritin@CuO2 (phage-Apo@CP) probe on an antimicrobial peptide (AMP)/MXenes-modified detection platform. With the specific recognition of AMP and phage-Apo@CP, the biosensor for the target Escherichia coli O157:H7 (E. coli O157:H7) presented multi-mode (bioluminescent, colorimetric, and electrochemical) signals to simultaneously ...
Tópico(s): Biosensors and Analytical Detection
2023 - Elsevier BV | Journal of Hazardous Materials
Shuai Wang, Jianhao Hu, Shu Xiao, Ming Wang, Jiale Yu, Zhijian Jia, Zhenzhong Yu, Ning Gan,
To ensure the safety of food, rapid screening and quantitative detection of foodborne pathogens are both significantly required. In this work, a dual-signal response biosensing strategy integrated with the visual fluorescent signal and the quantitative electrochemical signal was developed to simultaneously satisfy these demands. The deoxyribozyme (DNAzyme)-ferrocene immobilized on the MXene/gold nanobipyramid/antimicrobial peptide was employed as the signal probe for dual signal output. In the presence ...
Tópico(s): Bacteriophages and microbial interactions
2023 - Elsevier BV | Sensors and Actuators B Chemical
Hang You, Ming Wang, Shuai Wang, Jie Xu, Shuhao Hu, Tianhua Li, Zhenzhong Yu, Dianping Tang, Ning Gan,
Rapid, specific, and on-site detection of virulent foodborne pathogenic strains plays a key role in controlling food safety. In this work, an ultrasensitive and specific Phage@DNAzyme signal probe was designed to detect foodborne pathogens. The proposed sensing probe was composed of the selected phage and functionalized DNAzyme, which realized the specific recognition of target foodborne pathogens at the strain level and the efficient catalysis of copper(II) based azide-alkyne cycloaddition (CuAAC) ...
Tópico(s): SARS-CoV-2 detection and testing
2023 - American Chemical Society | Analytical Chemistry
Wenhai Wang, Shu Xiao, Mengli Zeng, Hong-Zhen Xie, Ning Gan,
Rapid and accurate detection of Vibrio parahaemolyticus (VP) in a variety of testing environments is critical for preventing the spread of foodborne illnesses. Therefore, a dual-mode colorimetric-electrochemical biosensor was prepared for the rapid and sensitive detection of VP. Phenylboronic acid-decorated CuO2 nanodot-mediated organic metal-organic framework (CP@MOF) nanozymes were constructed to produce colorimetric and electrochemical signals. Furthermore, an electrode surface was fabricated using ...
Tópico(s): Mosquito-borne diseases and control
2023 - Elsevier BV | Sensors and Actuators B Chemical
Ning Gan, Wei Qin, Chunlei Zhang, Ting Jiao,
This study proposed a one-step method for surface modification of dental implant, which showed excellent antibacterial effects, as well as improved osteogenic activities from osteogenic induction of cells and in situ mineralization of hydroxyapatite.
Tópico(s): Folate and B Vitamins Research
2022 - Royal Society of Chemistry | Journal of Materials Chemistry B
Liu Liu, Juncheng Hong, Wenhai Wang, Shu Xiao, Hong-Zhen Xie, Qiqin Wang, Ning Gan,
In this study, a fluorescent (FL) aptasensor was developed for on-site detection of live Salmonella typhimurium (S.T.) and Vibrio parahaemolyticus (V.P.). Complementary DNA (cDNA) of aptamer (Apt)-functionalized multicolor polyhedral oligomeric silsesquioxane-perovskite quantum dots (cDNA-POSS-PQDs) were used as encoded probes and combined with dual-stirring-bar-assisted signal amplification for pathogen quantification. In this system, bar 1 was labeled with the S.T. and V.P. Apts, and then bar 2 was ...
Tópico(s): Advanced Nanomaterials in Catalysis
2022 - Elsevier BV | Journal of Pharmaceutical Analysis
Binglin Mou, Chengyi Zuo, La Chen, Hong-Zhen Xie, Wentian Zhang, Qiqin Wang, Luhong Wen, Ning Gan,
In food safety monitoring, on-site and simultaneous detection of a variety of insecticides with different concentrations in the same matrix is necessary. However, the task remains challenging. In this study, a novel nitrogen and sulfur co-doped carbon dot (N, S-CD) was synthesized and used as a QuEChERS clean-up reagent to reduce matrix interferences in the determination of insecticides in vegetables. In addition, a portable mass spectrometer (µ-MS) was employed, without chromatography separation, ...
Tópico(s): Analytical chemistry methods development
2022 - Elsevier BV | Journal of Chromatography A
Jiaqi Wang, Lei Tan, Wenchao Bi, Hao-Yu Shen, Dengfeng Li, Zhenzhong Yu, Ning Gan,
This work demonstrated an ultrasensitive and simple microfluidic immunosensor for point-of-care test of Staphylococcus aureus (S. aureus) based on the stir bar enrichment and DNAzyme-assisted click reaction. Initially, S. aureus was enriched by the 4-mercaptophenylboronic acid-functionalized stir bar. The yolk antibody (immunoglobulin Y) and copper-labeled polydopamine nanoparticles were then specifically conjugated with the captured target. The Cu(II) was released under acidic conditions and effectively ...
Tópico(s): Advanced Biosensing Techniques and Applications
2022 - Elsevier BV | Food Chemistry
Cong Cao, Min Wang, Dingyi Zhang, Shaoning Yu, Hong-Zhen Xie, Qiqin Wang, Zhenzhong Yu, Ning Gan,
Live foodborne pathogens proliferate rapidly and do great harm to human health, which requires appropriate methods to supervise. In this work, a portable adenosine triphosphate (ATP) bioluminescence sensor with high specificity for live E. coli O157:H7 strain synergistically enhanced by orientated phage-modified stir bar extraction and bio-proliferation was developed. In brief, the selected phages were directionally immobilized on the poly(diallyldimethylammonium chloride)-modified gold stir bar ...
Tópico(s): Advanced biosensing and bioanalysis techniques
2022 - Elsevier BV | Biosensors and Bioelectronics
Jiale Yu, Shu Xiao, Zhenzhong Yu, Yufeng Hui, Tianhua Li, Dazhen Wu, Wenchao Bi, Ning Gan, Zhijian Jia,
Usually, a low concentration of pathogens proliferate rapidly in waters to cause serious threats to human health. It is necessary to develop on-site and speedy assays to detect them. In this work, a smart hydrogel aptasensor was assembled in Eppendorf tubes for quantifying Vibrio parahaemolyticus (V.P). It employed a dual-mode detection strategy combining visual fluorescence (FL) and microfluidic chip (MC) methods. In this aptasensor, the hydrogel contained ATP aptamer together with gold nanoclusters ( ...
Tópico(s): Aquaculture disease management and microbiota
2022 - Elsevier BV | Sensors and Actuators B Chemical
Shu Xiao, Xin Yang, Jiayi Wu, Qili Liu, Dengfeng Li, Shenfeng Huang, Hong-Zhen Xie, Zhenzhong Yu, Ning Gan,
Escherichia coli O157:H7 (E. coli) can proliferate and contaminate foods rapidly to cause serious hazards to consumers. Thus, developing a rapid on-site detection assay for E. coli is urgent. We prepared an egg yolk antibody (IgY) with high specificity and affinity for E. coli from eggs laid by specific pathogen-free (SPF) hens immunized with inactivated E. coli. Meanwhile, novel magnetic covalent organic framework (m-COF) microbeads with large specific area and abundant porosity were made to label ...
Tópico(s): SARS-CoV-2 detection and testing
2022 - Elsevier BV | Sensors and Actuators B Chemical
Tianliang Wang, Hong-Zhen Xie, Yuting Cao, Qing Xu, Ning Gan,
It is crucially important to develop simple, selective, and rapid extraction pretreatments and analytical methods to detect microcystins (MCs) in food samples with complex matrices. In this study, a magnetic self-assembled covalent organic framework (COF) adsorbent was synthesized via a facile approach by using a one-pot reaction of 1,6-bis(4-formylphenyl)-3,8-bis(4-aminophenyl)pyrene (BFBAPy) as precursors to assemble amino-functionalized magnetic Fe3O4-NH2 nanoparticles (Fe3O4@Py-COF, simplified as ...
Tópico(s): Covalent Organic Framework Applications
2022 - Elsevier BV | Journal of Chromatography A
B. J. Fineman, K.-Th. Brinkmann, A. L. Caraley, Ning Gan, Robert L. McGrath, J. Velkovska,
Proton and \ensuremath{\alpha}-particle spectral shapes and multiplicities have been measured in coincidence with evaporation residues from 145 to 220 MeV $^{28}\mathrm{Si}$${+}^{165}$Ho and 115 and 140 MeV $^{16}\mathrm{O}$${+}^{197}$Au${,}^{208}$Pb fusion reactions. Evaporation residues were separated using an electrostatic deflector and detected with large area surface barrier detectors. Light charged particles were detected at forward and backward angles with 14 single NaI detectors. In the context ...
Tópico(s): Radioactive contamination and transfer
1994 - American Institute of Physics | Physical Review C
Ning Gan, K.-Th. Brinkmann, A. L. Caraley, B. J. Fineman, Warnick J. Kernan, Robert L. McGrath, Paweł Danielewicz,
High-energy photon yields from the reactions $^{12}\mathrm{C}$${+}^{112,124}$Sn at 10 MeV/nucleon have been measured. The ratio of cross sections (${\mathit{E}}_{\ensuremath{\gamma}}$\ensuremath{\ge}30 MeV)\ensuremath{\sigma}${(}^{124}$Sn)/\ensuremath{\sigma}${(}^{112}$Sn) is 1.6\ifmmode\pm\else\textpm\fi{}0.2, which is larger than expected within the equal-participant model. The elementary n-p-\ensuremath{\gamma} cross section is evaluated within the neutral scalar \ensuremath{\sigma} meson exchange model, ...
Tópico(s): Nuclear Physics and Applications
1994 - American Institute of Physics | Physical Review C
K.-Th. Brinkmann, A. L. Caraley, B. J. Fineman, Ning Gan, J. Velkovska, Robert L. McGrath,
Evaporation residue excitation functions and \ensuremath{\alpha}-multiplicities from complete fusion of $^{16}\mathrm{O}$ with $^{197}\mathrm{Au}$ and $^{208}\mathrm{Pb}$ have been measured from the fusion threshold up to 140 MeV incident energy. The data allow the conclusion that strongly overdamped motion cannot be dominant at energies in the vicinity of the fusion barrier below ${\mathit{E}}^{\mathrm{*}}$\ensuremath{\approxeq}50 MeV. At excitation energies above 50 MeV the residue cross section for $^{16}\mathrm{ ...
Tópico(s): Nuclear reactor physics and engineering
1994 - American Institute of Physics | Physical Review C
In the context of severely restricted employment opportunities, undertaking an internship is an important way for students to enhance their employability and sustainable development. The aim of this study was to judge if the implementation of the Internship Promotion Programme (IPP) at universities in Zhejiang Province, China, could enhance graduates’ employability. We conducted surveys and interviews with 400 students and 100 business executives who participated in the IPP during the past 6 years. ...
Tópico(s): Entrepreneurship Studies and Influences
2021 - Multidisciplinary Digital Publishing Institute | Sustainability
Juncheng Hong, Wenhai Wang, Jiaqi Wang, Xiao Wang, Hong-Zhen Xie, Tianhua Li, Ning Gan,
Tópico(s): Perovskite Materials and Applications
2021 - Springer Science+Business Media | Microchimica Acta
Wenhai Wang, Shu Xiao, Zhijian Jia, Hong-Zhen Xie, Tianhua Li, Qiqin Wang, Ning Gan,
Foodborne pathogens could result in serious health issues to public and thus some rapid analysis methods are urgent developed to monitor them. Herein, a dual-mode aptasensor based on electrochemical and colorimetric modes was constructed for the on-site detection of Vibrio parahaemolyticus (V.P) in shrimps as a model. A novel nanoprobe ([email protected]@MXenes) was developed to generate both electrochemical and colorimetric signals by modifying phenylboronic acid and ferrocene on Pt-MXenes nanocomposite. ...
Tópico(s): Aquaculture disease management and microbiota
2021 - Elsevier BV | Sensors and Actuators B Chemical
Hongmei Zhong, Yong‐Xiang Wu, Shengrong Yu, Xiao Wang, Kangdi He, Dian Li, Yuting Cao, Ning Gan,
Biological fluorescence imaging technologies have attracted a lot of attention and have been widely used in biomedical fields. Compared with other technologies, fluorescence imaging has a lower cost, higher sensitivity, and easier operation. However, due to the disadvantages of one-photon (OP) fluorescence imaging, such as low spatial and poor temporal resolution and poor tissue permeability depth, the application of OP fluorescence imaging has some limitations. Though two-photon (TP) fluorescence ...
Tópico(s): Luminescence and Fluorescent Materials
2021 - American Chemical Society | Analytical Chemistry
Jianhao Hu, Zhipeng Shen, Lei Tan, Jun Yuan, Ning Gan,
In this work, we demonstrate a double stirring bars-based signal-amplified strategy for the simultaneous electrochemical detection of Vibrio parahaemolyticus (V.P) and Salmonella typhimurium (S.T). Methylene blue (MB) or ferrocene (Fc) labeled functionalized branched-chain DNA hybrid structure (FBCHS) was immobilized on magnetic microspheres and used as encoded signal tags. The encoded signal tags were hybridized with the aptamer-embedded tetrahedral DNA nanostructures (TDNs), which acted as capture probes, ...
Tópico(s): Bacteriophages and microbial interactions
2021 - Elsevier BV | Sensors and Actuators B Chemical
Jianhao Hu, Dazhen Wu, Tianhua Li, Yuting Cao, Xiao Wang, Ning Gan,
Tópico(s): Lipid Membrane Structure and Behavior
2021 - Elsevier BV | Sensors and Actuators B Chemical
Wenzhe Xing, Hui Zhou, Jingjing Han, You Zhou, Ning Gan, Jing Cuan,
The development of efficient and stable white-light emissive materials is highly desirable in displays and solid-state lighting. Here we present a high-quality white-light emitter based a dual-emitting MOF hybrid, which is achieved by dye encapsulation engineering within a robust Zr-MOF (PCN-128W) containing a highly luminescent tetraphenylethylene-based ligand. The pore confinement effect well isolates the incorporated dye molecules (trans-4-[4-(Dimethylamino)styryl]-1-methylpyridinium iodide (DSM)) ...
Tópico(s): Organic Light-Emitting Diodes Research
2021 - Elsevier BV | Journal of Colloid and Interface Science
Ning Gan, L.T. Liu, Xiongliang Yao, J.X. Wang, W. B. Wu,
A simplified open floating slender structure is used to investigate the dynamic behaviors of the warship in this paper. The deformation and damage mechanisms of the simplified open floating slender structure subjected to the underwater explosion are studied using the experiment and the Coupled Eulerian-Lagrangian (CEL) method. Firstly, a validation for the CEL method is carried out, where the numerical results agree well with the results in experiment. Subsequently, in order to analyze the deformation ...
Tópico(s): Energetic Materials and Combustion
2020 - Elsevier BV | Ocean Engineering
Fangfang Niu, Huafei Li, Xiaotao Xu, Lingling Sun, Ning Gan, Aizhong Wang,
Abstract Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a life‐threatening disease with a high mortality rate, which was a common complication of fat embolism syndrome (FES). Ursodeoxycholic acid (UDCA) has been reported to exert potent anti‐inflammatory effects under various conditions. In vivo , perinephric fat was injected via tail vein to establish a rat FES model, the anti‐inflammatory effects of UDCA on FES‐induced lung injury were investigated through histological examination, ...
Tópico(s): Chronic Obstructive Pulmonary Disease (COPD) Research
2020 - Wiley | Journal of Cellular and Molecular Medicine
Wenhai Wang, Lei Tan, Jiayi Wu, Tianhua Li, Hong-Zhen Xie, Dazhen Wu, Ning Gan,
Sensitive and rapid detection of pathogenic bacteria remains important and challenging for food safety and preventing outbreaks of foodborne disease. The major limitations of standard analytical methods for detecting vibrio parahaemolyticus (V.P) lie in their bulky equipment and tedious and long-time operation. This study presents an electrochemical aptasensor for the rapid on-site quantification of V.P in seafood. Magnetic nanoscale metal-organic frameworks (Fe3O4@NMOF) labeled with an aptamer against ...
Tópico(s): Biosensors and Analytical Detection
2020 - Elsevier BV | Analytica Chimica Acta
Liyong He, Zhipeng Shen, Jiaqi Wang, Jin Zeng, Wenhai Wang, Huihui Wu, Qiqin Wang, Ning Gan,
Tópico(s): DNA and Nucleic Acid Chemistry
2020 - Springer Science+Business Media | Microchimica Acta