Sarah R. Greytak, Ann M. Tarrant, Diane Nacci, Mark E. Hahn, Gloria V. Callard,
... populations, indicating that the adaptive mechanism is target gene-specific. Based on the lower basal levels of ERα mRNA in several tissues from adult NBH fish vs SC fish (Greytak and Callard, 2007), we predicted estrogen hyporesponsiveness; however, induction of ERα by estradiol exposure in reproductively inactive males did not differ between the two sites. Moreover, AroB was more responsive and Vtg induction was greater (2d) or similar (5d) in NBH as compared to SC males. Worth noting is the high inter-individual variability in estrogen responses of gene targets, especially in NBH killifish, which may indicate ...
Tópico(s): Fish Ecology and Management Studies
2010 - Elsevier BV | Aquatic Toxicology
... loss-of-function variants in human protein-coding genes.Science. 2012; 335:823–828.CrossrefMedlineGoogle Scholar4. Wooten EC, Bartleson V, Wolf MJ, Greytak SR, Orr N, Draper I, Calvino JE, Kapur ...
Tópico(s): Cardiovascular Function and Risk Factors
2013 - Lippincott Williams & Wilkins | Circulation Cardiovascular Genetics
Rosanne Rouf, Sarah R. Greytak, Eric C. Wooten, Jing Wu, Jay Boltax, Michael H. Picard, E. C. Svensson, Wolfgang Dillmann, Richard D. Patten, Gordon S. Huggins,
Reduced expression of sarcoplasmic reticulum calcium ATPase (SERCA)2 and other genes in the adult cardiac gene program has raised consideration of an impaired responsiveness to thyroid hormone (T3) that develops in the advanced failing heart. Here, we show that human and murine cardiomyopathy hearts have increased expression of friend of GATA (FOG)-2, a cardiac nuclear hormone receptor corepressor protein. Cardiac-specific overexpression of FOG-2 in transgenic mice led to depressed cardiac function, ...
Tópico(s): Metabolism, Diabetes, and Cancer
2008 - Lippincott Williams & Wilkins | Circulation Research
... question mark, because our current knowledge on the genes that determine facial shape is very limited. I don't think you can predict a face from DNA with any practically useful level of detail, accuracy, and reliability, as of yet,” he said. Greytak maintains that, when a case has grown cold, ...
Tópico(s): Animal Genetics and Reproduction
2015 - Springer Nature | EMBO Reports
Jason H. Moore, Peter C. Andrews, Randal S. Olson, Sarah E. Carlson, Curt R. Larock, Mario J. Bulhoes, James P.B. O’Connor, Ellen M. Greytak, Steven Armentrout,
Large-scale genetic studies of common human diseases have focused almost exclusively on the independent main effects of single-nucleotide polymorphisms (SNPs) on disease susceptibility. These studies have had some success, but much of the genetic architecture of common disease remains unexplained. Attention is now turning to detecting SNPs that impact disease susceptibility in the context of other genetic factors and environmental exposures. These context-dependent genetic effects can manifest themselves ...
Tópico(s):
2017 - BioMed Central | BioData Mining
Gordon S. Huggins, John J. Lepore, Sarah R. Greytak, Richard D. Patten, Rachel McNamee, Mark Aronovitz, Paul J. Wang, Guy L. Reed,
Signaling through cAMP plays an important role in heart failure. Phosphorylation of cAMP response element binding protein (CREB) at serine-133 regulates gene expression in the heart. We examined the functional significance of CREB-S133 phosphorylation by comparing transgenic models in which a phosphorylation resistant CREB-S133A mutant containing either an intact or a mutated leucine zipper domain (CREB-S133A-LZ) was expressed in the heart. In vitro, CREB-S133A retained the ability to interact with ...
Tópico(s): CRISPR and Genetic Engineering
2007 - American Physical Society | AJP Heart and Circulatory Physiology
Gloria V. Callard, Ann M. Tarrant, Apolonia Novillo, P. Yacci, Laura Ciaccia, Sándor Vajda, Gwo-Yu Chuang, Dima Kozakov, Sarah R. Greytak, Sherilyn J. Sawyer, Christopher Hoover, Kellie A. Cotter,
Classically, the estrogen signaling system has two core components: cytochrome P450 aromatase (CYP19), the enzyme complex that catalyzes the rate limiting step in estrogen biosynthesis; and estrogen receptors (ERs), ligand activated transcription factors that interact with the regulatory region of target genes to mediate the biological effects of estrogen. While the importance of estrogens for regulation of reproduction, development and physiology has been well-documented in gnathostome vertebrates, ...
Tópico(s): Marine Biology and Environmental Chemistry
2011 - Elsevier BV | The Journal of Steroid Biochemistry and Molecular Biology
Sarah R. Greytak, Gloria V. Callard,
Epidemiological, wildlife, and laboratory studies support the hypothesis that chemicals released into the environment through anthropogenic activities are responsible for abnormalities of reproduction and development. Although the New Bedford Harbor (NBH) killifish population has survived and reproduced successfully for >50 yr (∼20 generations), fish have high body burdens of the major NBH contaminants (polychlorinated biphenyls); elevated levels of P450 aromatase B and vitellogenin mRNA (markers of estrogen ...
Tópico(s): Effects and risks of endocrine disrupting chemicals
2006 - Elsevier BV | General and Comparative Endocrinology
Ann M. Tarrant, Sarah R. Greytak, Gloria V. Callard, Mark E. Hahn,
The estrogen receptor-related receptors (ERRs) are a group of nuclear receptors that were originally identified on the basis of sequence similarity to the estrogen receptors. The three mammalian ERR genes have been implicated in diverse physiological processes ranging from placental development to maintenance of bone density, but the diversity, function, and regulation of ERRs in non-mammalian species are not well understood. In this study, we report the cloning of four ERR cDNAs from the Atlantic ...
Tópico(s): Animal Behavior and Reproduction
2006 - Bioscientifica | Journal of Molecular Endocrinology
Eric C. Wooten, Virginia B. Hebl, Matthew J. Wolf, Sarah R. Greytak, Nicole M. Orr, Isabelle Draper, Jenna E. Calvino, Navin K. Kapur, Martin S. Maron, Iftikhar J. Kullo, Steve R. Ommen, J. Martijn Bos, Michael J. Ackerman, Gordon S. Huggins,
Incomplete penetrance and variable expression of hypertrophic cardiomyopathy (HCM) is well appreciated. Common genetic polymorphisms variants that may affect HCM penetrance and expression have been predicted but are not well established.We performed a case-control genomewide association study to identify common HCM-associated genetic polymorphisms and then asked whether such common variants were more represented in HCM or could explain the heterogeneity of HCM phenotypes. We identified an intronic ...
Tópico(s): Trypanosoma species research and implications
2012 - Lippincott Williams & Wilkins | Circulation Cardiovascular Genetics
Sarah R. Greytak, Denise Champlin, Gloria V. Callard,
Populations of killifish (Fundulus heteroclitus) persist in many different highly polluted environment indicative of adaptation or tolerance. In this study, we sought to determine whether long term, multigenerational exposures to environmental contaminants has affected reproductively relevant genes and biological processes. A homology cloning strategy was used to isolate the killifish cytochrome P450 aromatase (P450arom, estrogen synthetase) cDNAs. Consistent with previous fish studies, killifish ...
Tópico(s): Animal Genetics and Reproduction
2005 - Elsevier BV | Aquatic Toxicology
Cameron S. McAlpine, Geoff H. Werstuck,
... K. Zhou J. Doble B.W. Beahm C. Greytak S. Woulfe K. Trivedi C.M. Woodgett J. ...
Tópico(s): Phagocytosis and Immune Regulation
2014 - Elsevier BV | Journal of Lipid Research
Sarah R. Greytak, Kelly B. Engel, B. Paige Bass, Helen M. Moore,
Formalin-fixed and paraffin-embedded (FFPE) tissue biospecimens are a valuable resource for molecular cancer research. Although much can be gained from their use, it remains unclear whether the genomic and expression profiles obtained from FFPE biospecimens accurately reflect the physiologic condition of the patient from which they were procured, or if such profiles are confounded by biologic effects from formalin fixation and processing. To assess the physiologic accuracy of genomic and expression ...
Tópico(s):
2015 - American Association for Cancer Research | Cancer Research
Wendell Jones, Sarah R. Greytak, Hana M. Odeh, Ping Guan, Jason Powers, Jasmin Bavarva, Helen M. Moore,
Abstract The National Cancer Institute conducted the Biospecimen Pre-analytical Variables (BPV) study to determine the effects of formalin fixation and delay to fixation (DTF) on the analysis of nucleic acids. By performing whole transcriptome sequencing and small RNA profiling on matched snap-frozen and FFPE specimens exposed to different delays to fixation, this study aimed to determine acceptable delays to fixation and proper workflow for accurate and reliable Next-Generation Sequencing (NGS) analysis ...
Tópico(s): Cancer-related molecular mechanisms research
2019 - Nature Portfolio | Scientific Reports
Bruce Budowle, Magdalena M. Buś, Lynley F. Dungan, Angela van Daal,
... 154–162 (2016).Crossref, Medline, CAS, Google Scholar15. Greytak EM, Moore C, Armentrout SL. Genetic genealogy for ...
Tópico(s): Molecular Biology Techniques and Applications
2021 - Future Science Ltd | BioTechniques
Oriane Cédile, Sólja Remisdóttir Veyhe, Marcus Høy Hansen, Kjell Titlestad, Charlotte Guldborg Nyvold,
... 1408–1413 (2009).Crossref, Medline, CAS, Google Scholar6. Greytak SR, Engel KB, Parpart-Li S et al. ...
Tópico(s): Epigenetics and DNA Methylation
2021 - Future Science Ltd | BioTechniques
Sarah R. Greytak, Kelly B. Engel, Dave S.�B. Hoon, Kevin M. Elias, Christina M. Lockwood, Ping Guan, Helen M. Moore,
Abstract Circulating cell-free microRNAs (cfmiRNA) are an emerging class of biomarkers that have shown great promise in the clinical diagnosis, treatment, and monitoring of several pathological conditions, including cancer. However, validation and clinical implementation of cfmiRNA biomarkers has been hindered by the variability introduced during different or suboptimal specimen collection and handling practices. To address the need for standardization and evidence-based guidance, the National Cancer ...
Tópico(s): RNA Interference and
2023 - De Gruyter | Clinical Chemistry and Laboratory Medicine (CCLM)
Abstract Whole genome sequencing has opened the doors to Investigative genetic genealogy (IGG) analysis of challenging forensic samples that are not suitable for microarray genotyping. These samples still do not typically achieve high enough coverage for direct genotype calling, therefore a pipeline for imputation from low coverage sequencing data was evaluated using data from the 1000 Genomes Project. This pipeline generated results suitable for IGG down to 0.25X coverage. Additionally, forensic samples ...
Tópico(s): Genomics and Phylogenetic Studies
2022 - Elsevier BV | Forensic science international. Genetics supplement series
Dan Guo, Yaomin Xu, Zhangling Fan, Zhiyong Liang, Shiping Zhang, Craig J. Thomas, Samantha M. Thomas, J. N. L. Lamptey, R Indibi, Lacey Colligan, Wen Shao, Helena Judge Ellis, Mathias Brochhausen, Anna Maria Masci, Frank J. Manion, Jihad S. Obeid, Christian J. Stoeckert, Jie Zheng, Minhao Wu, Kaiyu Qian, Xiao Yun,
... to Improve Specimen QualityCampbell L. D., Engel K., Greytak S., Casas-Silva E., Guan P., Moore H. ...
Tópico(s): Ethics in Clinical Research
2018 - Mary Ann Liebert, Inc. | Biopreservation and Biobanking
Stephen Hermes, Janet Cady, Steven Armentrout, James P.B. O’Connor, Sarah Carlson Holdaway, Carlos Cruchaga, Thomas S. Wingo, Ellen M. Greytak,
Polygenic risk scores (PRS) are linear combinations of genetic markers weighted by effect size that are commonly used to predict disease risk. For complex heritable diseases such as late-onset Alzheimer's disease (LOAD), PRS models fail to capture much of the heritability. Additionally, PRS models are highly dependent on the population structure of the data on which effect sizes are assessed and have poor generalizability to new data.
Tópico(s):
2024 - IOS Press | Journal of Alzheimer s Disease
Leslie H. Sobin, Mary E. Barcus, Philip A. Branton, Kelly B. Engel, Judy Keen, David E. Tabor, Kristin Ardlie, Sarah R. Greytak, Nancy Roche, Brian T. Luke, Jim Vaught, Ping Guan, Helen M. Moore,
Context.— The National Institutes of Health Genotype-Tissue Expression (GTEx) project was developed to elucidate how genetic variation influences gene expression in multiple normal tissues procured from postmortem donors. Objective.— To provide critical insight into a biospecimen’s suitability for subsequent analysis, each biospecimen underwent quality assessment measures that included evaluation for underlying disease and potential effects introduced by preanalytic factors. Design.— Electronic images ...
Tópico(s): Molecular Biology Techniques and Applications
2024 - American Medical Association | Archives of Pathology & Laboratory Medicine
Philip A. Branton, Leslie H. Sobin, Mary E. Barcus, Kelly B. Engel, Sarah R. Greytak, Ping Guan, Jim Vaught, Helen M. Moore,
Context.— The National Institutes of Health (NIH) Genotype-Tissue Expression (GTEx) project was designed to evaluate how genetic variation and epigenetic effects influence gene expression in normal tissue. Objective.— To ensure that the grossly normal-appearing tissues collected were free from disease, each specimen underwent histologic evaluation. Design.— In total, nearly 30 000 tissue aliquots collected from almost 1000 postmortem donors underwent histologic review by project pathologists, and ...
Tópico(s): Molecular Biology Techniques and Applications
2024 - American Medical Association | Archives of Pathology & Laboratory Medicine