
Poliana S. Brasileiro, Juan C. Braga, Gilberto M. Amado‐Filho, Rachel Nunes Leal, Davide Bassi, Tarcila Franco, Alex Cardoso Bastos, Rodrigo L. Moura,
... Search Site Citation POLIANA S. BRASILEIRO, JUAN C. BRAGA, GILBERTO M. AMADO-FILHO, RACHEL N. LEAL, DAVIDE BASSI, ...
Tópico(s): Coral and Marine Ecosystems Studies
2018 - Society for Sedimentary Geology | Palaios

Douglas Galhardo, Regina Conceição Garcia, Cibele Regina Schneider, Gilberto Costa Braga, Émerson Dechechi Chambó, Daiane Luckmann Balbinotti de França, Sandra Mara Ströher,
The Southern region of Brazil is known for its floral diversity, presenting great potential for bee products such as honey, pollen, propolis and royal jelly. In this study, 67 honey samples provided by beekeepers from 14 municipalities of western Paraná were evaluated. Physicochemical parameters, bioactive compounds and antioxidant activity were analyzed. The physicochemical parameters of the samples were in compliance with the specifications by national and international standards, presenting average ...
Tópico(s): Phytochemicals and Antioxidant Activities
2020 - Sociedade Brasileira de Ciência e Tecnologia de Alimentos | Food Science and Technology

Gilberto Costa Braga, Priscilla Siqueira Melo, Keityane Boone Bergamaschi, Ana Paula Tiveron, Adna Prado Massarioli, Severino Matias de Alencar,
ABSTRACT: The objective of this study was to determine and correlate the extraction yields, antioxidant activity, total phenolics and total flavonoids from grape, mango and peanut agro-industrial by-products. The β-carotene/linoleic acid autoxidation system and scavenging capacity for DPPH and ABTS free radicals assays were used. The results were expressed in terms of lyophilized sample or dry extract. Mango bagasse exhibited the highest extraction yield (37.07%) followed by peanut skin (15.17%) and ...
Tópico(s): Antioxidant Activity and Oxidative Stress
2016 - UNIVERSIDADE FEDERAL DE SANTA MARIA | Ciência Rural

Viviane Marcela Celant, Gilberto Costa Braga, Jéssica Ariane Vorpagel, Ariane Busch Salibe,
ABSTRACT The objective of this work was to evaluate the content of phenolic compounds, anthocyanins and flavonoids extracted with 80% ethanol and water, and antioxidant capacity (DPPH and ABTS) of five blackberry cultivars produced in Western Paraná, Brazil. Brazos, Tupy, Arapaho, Choctaw and Guarani blackberries were studied. Soluble solids, titratable acidity and ascorbic acid of fruits were also characterized. Total phenolic compounds ranged from 8.23 to 14.98 mg GAE g-1 (f.w.) and Arapaho cultivar ...
Tópico(s): Phytochemistry Medicinal Plant Applications
2016 - Sociedade Brasileira de Fruticultura | Revista Brasileira de Fruticultura

Loreno Egídio Taffarel, Patrícia Barcellos Costa, Newton Tavares Escocard de Oliveira, Gilberto Costa Braga, Wilson João Zonin,
Este trabalho teve por objetivo avaliar o efeito dos sistemas de ordenha e de resfriamento na contagem bacteriana total (CBT) e na quantidade de leite com CBT < 10(5), que representa leite de alta qualidade. Um total de 12.221 registros de CBT do leite foi obtido de uma indústria láctea da região Oeste do Paraná, no período de janeiro a outubro de 2006. Após as coletas, a qualidade do leite foi analisada em laboratório pelo método de citometria de fluxo. Os dados de CBT foram classificados por sistema ...
Tópico(s): Food Safety and Hygiene
2013 - Zeppelini Editorial | Arquivos do Instituto Biológico

Maria Cristina Copello Rotili, Jéssica Ariane Vorpagel, Gilberto Costa Braga, Odair José Kuhn, Ariane Busch Salibe,
O objetivo deste trabalho foi avaliar as características químicas e a conservação do maracujá-amarelo embalado com filme de PVC durante o armazenamento refrigerado a 5 ºC. Atividade antioxidante (DPPH e TEAC), compostos fenólicos totais, β-caroteno e ácido ascórbico do suco do fruto foram os parâmetros químicos avaliados. As estimativas de perda de massa, enrugamento, cor e sintomas de patógenos foram utilizadas no estudo de conservação. As avaliações foram realizadas em intervalos de 10 dias, durante ...
Tópico(s): Banana Cultivation and Research
2013 - Sociedade Brasileira de Fruticultura | Revista Brasileira de Fruticultura

Nilton Garcia Marengoni, Magali Soares dos Santos Pozza, Gilberto Costa Braga, Douglas Batista Lazzeri, Leandro Dalcin Castilha, Guilherme Wolff Bueno, Tiago Júnior Pasquetti, Clauber Polese,
This work aimed to evaluate the microbiological, sensory and centesimal characterization of four kinds of fishburgers elaborated with tilapia mechanically separated meat. The microbiological analyses showed that both raw material and fishburgers met the microbiological standards for products derived from fish and certified that the use of byproducts derived from fish processing is a form of aggregating value to a final product, with good acceptance by the consumer, assisting quality standards. It ...
Tópico(s): Food Safety and Hygiene
2009 - UNIVERSIDADE FEDERAL DA BAHIA | Revista Brasileira de Saúde e Produção Animal

Paola Kiara Guerreiro, M. R. F. Machado, Gilberto Costa Braga, Eliane Gasparino, Alexandra da Silva Martinez Franzener,
A indústria leiteira atravessa um período de intensas transformações em sua estrutura, e a qualidade do leite é uma das principais exigências. A correta adoção de medidas de higiene adequadas na produção, armazenamento e transporte do leite podem prevenir a contaminação de microrganismos psicrotróficos que representam um grave problema econômico para a indústria de laticínios. Desta forma, este estudo realizou-se com o objetivo de avaliar o efeito de diferentes técnicas profiláticas de higiene e ...
Tópico(s): Enterobacteriaceae and Cronobacter Research
2005 - UNIVERSIDADE FEDERAL DE LAVRAS | Ciência e Agrotecnologia

Gilberto C. Braga, Sandra M. Couto, Tetuo Hara, Jayme T.P. Almeida Neto,
The operation of cracking Macadamia nuts is the most critical and delicate step for achieving high-quality kernels. The nuts, usually at high moisture content, are dried to a recommended moisture, before cracking, in a process that takes from 3 to 4 weeks at the expense of large energy consumption. The present paper examines the basis of shell rupture which is needed for the development of new methodologies or techniques to reduce the drying period and to obtain a more efficient kernel extraction ...
Tópico(s): Tree Root and Stability Studies
1999 - Elsevier BV | Journal of Agricultural Engineering Research

Marco Túlio Lopes Caldas, Gilberto Ferreira Braga, Samuel Mendes, Juliano Martins da Silveira, Robson Massi Kopke,
To investigate the prevalence and combinations of PCL injuries and their correlations with the mechanism, the occurrence of evident dislocation and associated fracture. A retrospective study of 85 lesions of PCL operated between 2003 and 2010. Diagnosis by physical examination and dynamic radiography, compared with surgical findings. Injuries involving the PCL were more prevalent in men (78.8%) with a mean age of 33 years. The main cause was traffic accidents (73.80%), and (49.4%) motorcycle. Isolated ...
Tópico(s): Orthopedic Surgery and Rehabilitation
2013 - Brazilian Society of Orthopedics and Traumatology | Revista Brasileira de Ortopedia (English Edition)

Ariane Busch Salibe, Gilberto Costa Braga, Rafael Pio, Cláudio Yuji Tsutsumi, Paulo Evandro Jandrey, Charles Douglas Rossol, Jean Roger da Silva Fréz, Tânia Pires da Silva,
O presente trabalho teve como objetivo estudar o enraizamento de estacas de porta-enxerto 'VR 043-43' (V. vinifera x V. rotundifolia), testando diferentes concentrações de ácido indolbutírico (0, 1.000, 2.000 e 3.000 mg L-1), ácido bórico (0 e 150 µg L-1) e a técnica de estratificação a 4 ºC por 48 horas em câmara refrigerada. Utilizou-se o delineamento experimental em blocos inteiramente casualizados, em esquema fatorial 4 x 2 x 2, com cinco repetições e cada parcela representada por oito estacas. ...
Tópico(s): Plant Physiology and Cultivation Studies
2010 - INSTITUTO AGRONÔMICO DE CAMPINAS | Bragantia

Alessandra Maria Detoni, Edmar Clemente, Gilberto Costa Braga, Neuza Francisca Michelon Herzog,
O sistema de cultivo, as cultivares, as condições de colheita e pós-colheita têm efeito direto na conservação e qualidade das uvas. Diante disso, este trabalho objetivou avaliar o efeito do tempo (0, 7, 14, 21, 28 e 35 dias) e da temperatura de armazenamento (1, 14 e 24ºC) na qualidade pós-colheita da uva "Niágara Rosada" cultivada no sistema orgânico. O fator limitante para o armazenamento foi o elevado índice de degrana, porém, as uvas ainda possuíam qualidade para o consumo, por observar a manutenção ...
Tópico(s): Postharvest Quality and Shelf Life Management
2005 - Sociedade Brasileira de Ciência e Tecnologia de Alimentos | Food Science and Technology

Fabiana Roberta Segura, Emilene Arusievicz Nunes, Fernanda Pollo Paniz, Ana Carolina Cavalheiro Paulelli, Gabriela Braga Rodrigues, Gilberto Úbida Leite Braga, Walter dos Reis Pedreira Filho, Fernando Barbosa, Giselle Cerchiaro, Fábio Ferreira Silva, Bruno Lemos Batista,
On November 5th, 2015, Samarco's iron mine dam – called Fundão – spilled 50–60 million m3 of mud into Gualaxo do Norte, a river that belongs to Rio Doce Basin. Approximately 15 km2 were flooded along the rivers Gualaxo do Norte, Carmo and Doce, reaching the Atlantic Ocean on November 22nd, 2015. Six days after, our group collected mud, soil and water samples in Bento Rodrigues (Minas Gerais, Brazil), which was the first impacted area. Overall, the results, water samples – potable and surface water from ...
Tópico(s): Mining and Resource Management
2016 - Elsevier BV | Environmental Pollution

Jocélio dos Santos Araújo, Vladimir de Oliveira, Gilberto Costa Braga,
This experiment aimed to determine the effect of three different types of litter and two stocking density (10 and 12 birds/m2) in the performance of broilers in two phases (1 to 28 days and 29 to 42 days). 264 Hubbard male and female chicks were allotted in randomized blocks design in a 3 x 2 x 2 factorial arrangement with four replications per treatment. The parameters evaluated were: weight gain, feed intake and feed to gain ratio. No significative effect (P>0.05) was observed of types of litter ...
Tópico(s): Animal Nutrition and Physiology
2007 - UNIVERSIDADE FEDERAL DE GOIÁS | Ciência Animal Brasileira

Gabriela Braga Rodrigues, Liana K.S. Ferreira, Mark Wainwright, Gilberto Úbida Leite Braga,
Photodynamic antimicrobial chemotherapy (PACT) is a promising alternative to conventional chemotherapy that can be used to treat localized mycosis. The development of PACT depends on identifying effective and selective PS for the different pathogenic species. The in vitro susceptibilities of Trichophyton mentagrophytes and Trichophyton rubrum microconidia to PACT with methylene blue (MB), toluidine blue O (TBO), new methylene blue N (NMBN), and the novel pentacyclic phenothiazinium photosensitizer S137 ...
Tópico(s): Fungal Infections and Studies
2012 - Elsevier BV | Journal of Photochemistry and Photobiology B Biology

Gabriela Braga Rodrigues, Fernando Lucas Primo, Antônio Cláudio Tedesco, Gilberto Úbida Leite Braga,
Abstract The selection of fungi resistant to currently used fungicides and the emergence of new pathogenic species make the development of alternative fungus‐control techniques highly desirable. Photodynamic antimicrobial chemotherapy (PACT) is a promising method which combines a nontoxic photosensitizer (PS) with visible light to cause selective killing of microbial cells. The development of PACT to treat mycoses or kill fungi in the environment depends on identifying effective PS for the different ...
Tópico(s): Photodynamic Therapy Research Studies
2011 - Wiley | Photochemistry and Photobiology

Júlia Cunha Gonzales, Guilherme Thomaz Pereira Brancini, Gabriela Braga Rodrigues, Geraldo José da Silva, Luciano Bachmann, Mark Wainwright, Gilberto Úbida Leite Braga,
Antimicrobial photodynamic treatment (APDT) is a promising light based approach to control diseases caused by plant-pathogenic fungi. In the present study, we evaluated the effects of APDT with the phenothiazinium photosensitizer methylene blue (MB) under solar radiation on the germination and viability of conidia of the pathogenic fungus Colletotricum abscissum (former Colletotrichum acutatum sensu lato). Experiments were performed both on petals and leaves of sweet orange (Citrus sinensis) in different ...
Tópico(s): Fungal Infections and Studies
2017 - Elsevier BV | Journal of Photochemistry and Photobiology B Biology

Gabriela Braga Rodrigues, Marcelo Dias‐Baruffi, Natalie S. Holman, Mark Wainwright, Gilberto Úbida Leite Braga,
Tópico(s): Nanoplatforms for cancer theranostics
2012 - Elsevier BV | Photodiagnosis and Photodynamic Therapy

Henrique Dantas de Menezes, Gabriela Braga Rodrigues, Simone de Pádua Teixeira, Nelson Sidnei Massola Júnior, Luciano Bachmann, Mark Wainwright, Gilberto Úbida Leite Braga,
The increasing tolerance to currently used fungicides in both clinical and agricultural areas is of great concern. The nonconventional light-based approach of antimicrobial photodynamic treatment (APDT) is a promising alternative to conventional fungicides. We evaluated the effects of APDT with four phenothiazinium derivatives (methylene blue [MB], new methylene blue N [NMBN], toluidine blue O [TBO], and the novel pentacyclic phenothiazinium photosensitizer [PS] S137) on conidia of three fungal species ( ...
Tópico(s): Ocular Infections and Treatments
2013 - American Society for Microbiology | Applied and Environmental Microbiology

Gabriela Braga Rodrigues, Guilherme Thomaz Pereira Brancini, Marcelo R. Pinto, Fernando Lucas Primo, Mark Wainwright, Antônio Cláudio Tedesco, Gilberto Úbida Leite Braga,
The in vitro susceptibilities of Candida albicans and Candida tropicalis to Antimicrobial Photodynamic Treatment with aluminum phthalocyanine chloride in nanoemulsion (ClAlPc/NE) were investigated. PS concentration- and fluence-dependent cell survival after APDT were compared before and after unbound extracellular PS had been washed out. The PS uptake and its subcellular localization were also determined. Exposure to light in the absence of the PS and treatment with the PS in the absence of light ...
Tópico(s): Ocular Infections and Treatments
2019 - Elsevier BV | Fungal Biology

Guilherme Thomaz Pereira Brancini, Gabriela Braga Rodrigues, Mariana de Souza Lima Rambaldi, Clarice Izumi, Ana Patrícia Yatsuda, Mark Wainwright, José César Rosa, Gilberto Úbida Leite Braga,
Candida albicans is a human pathogenic fungus mainly affecting immunocompromised patients. Resistance to the commonly used fungicides can lead to poor treatment of mucosal infections which, in turn, can result in life-threatening systemic candidiasis. In this scenario, antimicrobial photodynamic treatment (PDT) has emerged as an effective alternative to treat superficial and localized fungal infections. Microbial death in PDT is a consequence of the oxidation of many cellular biomolecules, including ...
Tópico(s): Biosensors and Analytical Detection
2016 - Springer Science+Business Media | Photochemical & Photobiological Sciences

Letícia Fracarolli, Gabriela Braga Rodrigues, Ana C. Pereira, Nelson Sidnei Massola Júnior, Geraldo José da Silva, Luciano Bachmann, Mark Wainwright, Jairo Kenupp Bastos, Gilberto Úbida Leite Braga,
The increasing tolerance to currently used fungicides and the need for environmentally friendly antimicrobial approaches have stimulated the development of novel strategies to control plant-pathogenic fungi such as antimicrobial phototreatment (APT). We investigated the in vitro APT of the plant-pathogenic fungus Colletotrichum acutatum with furocoumarins and coumarins and solar radiation. The compounds used were: furocoumarins 8-methoxypsoralen (8-MOP) and 5,8-dimethoxypsoralen (isopimpinellin), coumarins ...
Tópico(s): Mycotoxins in Agriculture and Food
2016 - Elsevier BV | Journal of Photochemistry and Photobiology B Biology

Shirlene Souza Oliveira, Gilberto Costa Braga, Noélle Khristinne Cordeiro, José Renato Stangarlin, Helton José Alves,
Silver nanoparticles (AgNPs) can be produced through an easy and safe process called green synthesis and have been considered an efficient antimicrobial agent. The antimicrobial effect of silver nanoparticles green synthesized with E. tirucalli (aveloz) can be a promising technique for preserving stored strawberries. The objective of this work was to perform a green synthesis of AgNPs with aveloz extract (Av) and evaluate its effect on the physiology and preservation of stored strawberries. Silver ...
Tópico(s): Essential Oils and Antimicrobial Activity
2021 - Springer Science+Business Media | Journal of Food Science and Technology

Marcelo Andreotti, Roberto Natal Dal Molin, André Rodrigues dos Reis, Enes Furlani, Gilberto Costa Braga, Maria Brígida dos Santos Scholz,
Climatic variations influence formation and maturation of coffee grains by altering their intrinsic characteristics, which can allow for several types of coffee qualities, including the potential for production of special coffee. This study was carried out to verify the effect of environmental conditions and crop cultivation on chemical composition and their consequences in cup quality of coffees from region of Jesuítas, Paraná State. During the same crop season this study was accomplished (2002- ...
Tópico(s): Coffee research and impacts
2008 - Editora da Universidade Estadual de Maringá (Eduem) | Acta Scientiarum Agronomy

Gilberto Úbida Leite Braga, Geraldo José da Silva, Guilherme Thomaz Pereira Brancini, John E. Hallsworth, Mark Wainwright,
Classical approaches for controlling plant pathogens may be impaired by the development of pathogen resistance to chemical pesticides and by limited availability of effective antimicrobial agents. Recent increases in consumer awareness of and/or legislation regarding environmental and human health, and the urgent need to improve food security, are driving increased demand for safer antimicrobial strategies. Therefore, there is a need for a step change in the approaches used for controlling pre- ...
Tópico(s): Antifungal resistance and susceptibility
2022 - Elsevier BV | Journal of Photochemistry and Photobiology B Biology

Patrícia Helena Grizante Barião, Ludmilla Tonani, Guilherme Thomaz Pereira Brancini, Érika Nascimento, Gilberto Úbida Leite Braga, Mark S. Wainwright, Márcia Regina von Zeska Kress,
The fast-emerging and multidrug-resistant Candida auris is the first fungal pathogen to be considered a threat to global public health. Thus, there is a high unmet medical need to develop new therapeutic strategies to control this species. Antimicrobial photodynamic therapy (APDT) is a promising alternative that simultaneously targets and damages numerous microbial biomolecules. Here, we investigated the in vitro and in vivo effects of APDT with four phenothiazinium photosensitizers: (i) methylene ...
Tópico(s): Phenothiazines and Benzothiazines Synthesis and Activities
2022 - Springer Science+Business Media | Photochemical & Photobiological Sciences

Nathália Abichabki, Luísa Vieira Zacharias, Natália Columbaro Moreira, Fernando Bellissimo‐Rodrigues, Fernanda de Lima Moreira, Jhohann R. L. Benzi, Tânia M. C. Ogasawara, Joseane Cristina Ferreira, Camila M. Ribeiro, Fernando R. Pavan, Leonardo Reis Leira Pereira, Guilherme Thomaz Pereira Brancini, Gilberto Úbida Leite Braga, Antônio Waldo Zuardi, Jaime E. C. Hallak, J.A.S. Crippa, Vera Lúcia Lanchote, Rafael Cantón, Ana Lúcia da Costa Darini, Leonardo Neves Andrade,
Abstract This study aimed to assess the ultrapure cannabidiol (CBD) antibacterial activity and to investigate the antibacterial activity of the combination CBD + polymyxin B (PB) against Gram-negative (GN) bacteria, including PB-resistant Gram-negative bacilli (GNB). We used the standard broth microdilution method, checkerboard assay, and time-kill assay. CBD exhibited antibacterial activity against Gram-positive bacteria, lipooligosaccharide (LOS)-expressing GN diplococcus (GND) ( Neisseria gonorrhoeae , Neisseria ...
Tópico(s): Phenothiazines and Benzothiazines Synthesis and Activities
2022 - Nature Portfolio | Scientific Reports

Caetano P. Sabino, Martha S. Ribeiro, Mark Wainwright, Carolina dos Anjos, Fábio P. Sellera, Milena Dropa, Nathalia B. Nunes, Guilherme Thomaz Pereira Brancini, Gilberto Úbida Leite Braga, Victor E. Arana‐Chavez, Raul O. Freitas, Nilton Lincopán, Maurı́cio S. Baptista,
The unbridled dissemination of multidrug-resistant pathogens is a major threat to global health and urgently demands novel therapeutic alternatives. Antimicrobial photodynamic therapy (aPDT) has been developed as a promising approach to treat localized infections regardless of drug resistance profile or taxonomy. Even though this technique has been known for more than a century, discussions and speculations regarding the biochemical mechanisms of microbial inactivation have never reached a consensus ...
Tópico(s): Ocular Infections and Treatments
2022 - Wiley | Photochemistry and Photobiology

Guilherme Thomaz Pereira Brancini, John E. Hallsworth, Luis M. Corrochano, Gilberto Úbida Leite Braga,
Metarhizium fungi are soil-inhabiting ascomycetes which are saprotrophs, symbionts of plants, pathogens of insects, and participate in other trophic/ecological interactions, thereby performing multiple essential ecosystem services. Metarhizium species are used to control insect pests of crop plants and insects that act as vectors of human and animal diseases. To fulfil their functions in the environment and as biocontrol agents, these fungi must endure cellular stresses imposed by the environment, ...
Tópico(s): Insect and Arachnid Ecology and Behavior
2021 - Elsevier BV | Journal of Photochemistry and Photobiology B Biology

Leonardo do Prado‐Silva, Guilherme Thomaz Pereira Brancini, Gilberto Úbida Leite Braga, Xinyu Liao, Tian Ding, Anderson S. Sant’Ana,
Antimicrobial photodynamic treatment (aPDT) is a light-based method developed for the inactivation of microorganisms. With the emergence of antifungal- and antibiotic-resistant microorganisms, aPDT has been suggested as a promising microbial control strategy. The treatment is based on a combination of visible light, a photosensitizer (PS), and molecular oxygen. Illuminating the PS with adequate light in the presence of oxygen will result in the generation of abundant reactive oxygen species (ROS), ...
Tópico(s): Biosensors and Analytical Detection
2021 - Elsevier BV | Food Control