Produção Nacional
Revisado por pares
Gileade Pereira Freitas, Helena Bacha Lopes, Alann Thaffarell Portilho Souza, Maria Paula Oliveira Gomes, Georgia K. Quiles, Jonathan Gordon, Coralee E. Tye, Janet L. Stein, Gary S. Stein, Jane B. Lian, Márcio Mateus Beloti, Adalberto Luiz Rosa,
Cell therapy is a valuable strategy for the replacement of bone grafts and repair bone defects, and mesenchymal stem cells (MSCs) are the most frequently used cells. This study was designed to genetically edit MSCs to overexpress bone morphogenetic protein 9 (BMP-9) using Clustered Regularly Interspaced Short Palindromic Repeats/associated nuclease Cas9 (CRISPR-Cas9) technique to generate iMSCs-VPRBMP-9+, followed by in vitro evaluation of osteogenic potential and in vivo enhancement of bone formation ...
Tópico(s): Renal and related cancers
2021 - Springer Nature | Gene Therapy
Produção Nacional
Revisado por pares
Gileade Pereira Freitas, Alann Thaffarell Portilho Souza, Helena Bacha Lopes, Rayana Longo Bighetti‐Trevisan, Fabíola Cristina Ribeiro de Oliveira, Roger Rodrigo Fernandes, Fernanda Silva Ferreira, Felipe Augusto Rós, Márcio Mateus Beloti, Adalberto Luiz Rosa,
Since their discovery, mesenchymal stromal cells (MSCs) have received a lot of attention, mainly due to their self-renewal potential and multilineage differentiation capacity. For these reasons, MSCs are a useful tool in cell biology and regenerative medicine. In this article, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and adipose tissues (AT-MSCs), and methods to culture, characterize, and differentiate MSCs into osteoblasts, adipocytes, and chondrocytes. After the harvesting ...
Tópico(s): Bone fractures and treatments
2020 - American Academy of Arts and Sciences | BIO-PROTOCOL
Produção Nacional
Revisado por pares
Helena Bacha Lopes, Alann Thaffarell Portilho Souza, Gileade Pereira Freitas, Carlos Nelson Elias, Adalberto Luiz Rosa, Márcio Mateus Beloti,
The present study aimed to investigate the participation of focal adhesion kinases (FAK) in interactions between osteoblastic cells and titanium (Ti) surfaces with three different topographies, namely, untreated (US), microstructured (MS), and nanostructured (NS).Osteoblasts harvested from the calvarial bones of 3-day-old rats were cultured on US, MS and NS discs in the presence of PF-573228 (FAK inhibitor) to evaluate osteoblastic differentiation. After 24 h, we evaluated osteoblast morphology and vinculin ...
Tópico(s): Bone Metabolism and Diseases
2020 - UNIVERSIDADE DE SÃO PAULO | Journal of Applied Oral Science
Produção Nacional
Revisado por pares
Helena Bacha Lopes, Gileade Pereira Freitas, Daianne Maciely Carvalho Fantacini, Virgínia Picanço‐Castro, Dimas Tadeu Covas, Adalberto Luiz Rosa, Márcio Mateus Beloti,
Topographical modifications of titanium (Ti) at the nanoscale level generate surfaces that regulate several signaling pathways and cellular functions, which may affect the process of osseointegration. Here, we investigated the participation of integrin αV in the osteogenic capacity of Ti with nanotopography. Machined titanium discs (untreated) were submitted to treatment with H2 SO4 /H2 O2 to produce the nanotopography (nanostructured). First, the greater osteogenic capacity of the nanotopography that ...
Tópico(s): Bone Tissue Engineering Materials
2019 - Wiley | Journal of Cellular Biochemistry
Produção Nacional
Revisado por pares
Helena Bacha Lopes, Gileade Pereira Freitas, Carlos Nelson Elias, Coralee E. Tye, Janet L. Stein, Gary S. Stein, Jane B. Lian, Adalberto Luiz Rosa, Márcio Mateus Beloti,
Abstract The major role of integrins is to mediate cell adhesion but some of them are involved in the osteoblasts–titanium (Ti) interactions. In this study, we investigated the participation of integrins in osteoblast differentiation induced by Ti with nanotopography (Ti‐Nano) and with microtopography (Ti‐Micro). By using a PCR array, we observed that, compared with Ti‐Micro, Ti‐Nano upregulated the expression of five integrins in mesenchymal stem cells, including integrin β3, which increases osteoblast ...
Tópico(s): Cell Adhesion Molecules Research
2019 - Wiley | Journal of Biomedical Materials Research Part A
Produção Nacional
Revisado por pares
Gileade Pereira Freitas, Helena Bacha Lopes, Alann Thaffarell Portilho Souza, Paula Gabriela Faciola Pessôa de Oliveira, Adriana Luisa Gonçalves Almeida, Paulo G. Coelho, Fernanda Ursoli Ferreira, Dimas Tadeu Covas, Márcio Mateus Beloti, Adalberto Luiz Rosa,
Aim: The aim of this study was to investigate the effect of local injection of osteoblasts differentiated from bone marrow (BM-OB) or adipose tissue (AT-OB) mesenchymal stromal cells on bone tissue formation. Materials & methods: Defects were created in rat calvaria and injected with BM-OB or AT-OB and phosphate-buffered saline without cells were injected as control. Bone formation was evaluated 4 weeks postinjection. Results: Injection of BM-OB or AT-OB resulted in higher bone formation than that obtained ...
Tópico(s): Bone fractures and treatments
2019 - Future Medicine | Regenerative Medicine
Produção Nacional
Revisado por pares
Rodrigo Paolo Flores Abuna, Fabíola Singaretti Oliveira, Helena Bacha Lopes, Gileade Pereira Freitas, Roger Rodrigo Fernandes, Adalberto Luiz Rosa, Márcio Mateus Beloti,
Wnt/β-catenin signal transduction is involved in the homeostatic control of bone mass. It is well established that a titanium surface with nanotopography (Ti-Nano) favors osteoblast differentiation by modulating different signaling pathways. However, few studies have investigated the participation of the Wnt/β-catenin pathway in the osteogenic effect of nanoscale topographies. In this study, we aimed to determine whether the Wnt/β-catenin signaling pathway is involved in the elevated osteogenic potential ...
Tópico(s): Bone Metabolism and Diseases
2019 - Elsevier BV | Colloids and Surfaces B Biointerfaces
Produção Nacional
Revisado por pares
Gileade Pereira Freitas, Helena Bacha Lopes, Alann Thaffarell Portilho Souza, Paula Gabriela Faciola Pessôa de Oliveira, Adriana Luisa Gonçalves Almeida, Lucas Eduardo Botelho de Souza, Paulo G. Coelho, Márcio Mateus Beloti, Adalberto Luiz Rosa,
Abstract Treatment of large bone defects is a challenging clinical situation that may be benefited from cell therapies based on regenerative medicine. This study was conducted to evaluate the effect of local injection of bone marrow-derived mesenchymal stromal cells (BM-MSCs) or adipose tissue-derived MSCs (AT-MSCs) on the regeneration of rat calvarial defects. BM-MSCs and AT-MSCs were characterized based on their expression of specific surface markers; cell viability was evaluated after injection ...
Tópico(s): Bone fractures and treatments
2019 - Nature Portfolio | Scientific Reports
Produção Nacional
Revisado por pares
Alann Thaffarell Portilho Souza, Barbara L.S. Bezerra, Fabíola Singaretti Oliveira, Gileade Pereira Freitas, Rayana Longo Bighetti‐Trevisan, Paulo Tambasco de Oliveira, Adalberto Luiz Rosa, Márcio Mateus Beloti,
Abstract Among bone morphogenetic proteins (BMPs), BMP‐9 has been described as one with higher osteogenic potential. Here, we aimed at evaluating the effect of BMP‐9 on the osteoblast differentiation of cells grown on titanium (Ti) with nanotopography, a well‐known osseoinductive surface. MC3T3‐E1 cells were grown either in absence or presence of BMP‐9 (20 nM) on Ti with nanotopography (Ti‐Nano) or machined Ti (Ti‐Machined) for up to 21 days to evaluate the gene expression of RUNX2, osterix, osteocalcin, ...
Tópico(s): Orthopaedic implants and arthroplasty
2018 - Wiley | Journal of Cellular Biochemistry
Produção Nacional
Revisado por pares
Rodrigo Paolo Flores Abuna, Fabíola Singaretti Oliveira, Jaqueline Isadora Reis Ramos, Helena Bacha Lopes, Gileade Pereira Freitas, Alann Thaffarell Portilho Souza, Márcio Mateus Beloti, Adalberto Luiz Rosa,
Quantitative real‐time polymerase chain reaction (qRT‐PCR) is a powerful tool to evaluate gene expression, but its accuracy depends on the choice and stability of the reference genes used for normalization. In this study, we aimed to identify reference genes for studies on osteoblasts derived from rat bone marrow mesenchymal stem cells (bone marrow osteoblasts), osteoblasts derived from newborn rat calvarial (calvarial osteoblasts), and rat osteosarcoma cell line UMR‐106. The osteoblast phenotype was ...
Tópico(s): Virus-based gene therapy research
2018 - Wiley | Journal of Cellular Physiology
Produção Nacional
Revisado por pares
Alann Thaffarell Portilho Souza, Gileade Pereira Freitas, Helena Bacha Lopes, Emanuela Prado Ferraz, Fabíola Singaretti Oliveira, Márcio Mateus Beloti, Adalberto Luiz Rosa,
Abstract Background aims Regenerative medicine strategies based on cell therapy are considered a promising approach to repair bone defects. The aims of this study were to evaluate the effect of subculturing on the osteogenic potential of osteoblasts derived from newborn rat calvaria and the effect of these osteoblasts on bone repair of rat calvaria defects. Methods Cells were obtained from 50 newborn rat calvaria, and primary osteoblasts (OB) were compared with first passage (OB-P1) in terms of osteogenic ...
Tópico(s): Bone Tissue Engineering Materials
2018 - Elsevier BV | Cytotherapy
Produção Nacional
Revisado por pares
Gileade Pereira Freitas, Helena Bacha Lopes, Adriana Luisa Gonçalves Almeida, Rodrigo Paolo Flores Abuna, Rossano Gimenes, Lucas Eduardo Botelho de Souza, Dimas Tadeu Covas, Márcio Mateus Beloti, Adalberto Luiz Rosa,
Tópico(s): Bone fractures and treatments
2017 - Springer Science+Business Media | Calcified Tissue International
Produção Nacional
Revisado por pares
Emanuela Prado Ferraz, Gileade Pereira Freitas, Murilo C. Crovace, Oscar Peitl, Edgar D. Zanotto, Paulo Tambasco de Oliveira, Márcio Mateus Beloti, Adalberto Luiz Rosa,
We aimed to evaluate the in vitro osteogenic and osteoinductive potentials of BioS-2P and its ability to promote in vivo bone repair. To investigate osteogenic potential, UMR-106 osteoblastic cells were cultured on BioS-2P and Bioglass 45S5 discs in osteogenic medium. The osteoinductive potential was evaluated using mesenchymal stem cells (MSCs) cultured on BioS-2P, Bioglass 45S5 and polystyrene in non-osteogenic medium. Rat bone calvarial defects were implanted with BioS-2P scaffolds alone or seeded ...
Tópico(s): Dental materials and restorations
2017 - IOP Publishing | Biomedical Materials
Gileade Pereira Freitas, Ronaldo Hirata, Estevam A. Bonfante, Nick Tovar, Paulo G. Coelho,
To investigate the probability of survival of different implant-abutment connection designs in narrow versus standard-diameter implants supporting anterior crowns.A total of 108 implants of either 3.5-mm or 4.0-mm diameter (narrow and standard, respectively) (10 mm in length, Implacil de Bortoli) were divided into six groups (n=18 each) as follows: external hexagon 3.5 mm or 4.0 mm (EH3.5 or EH4.0), internal hexagon 3.5 mm or 4.0 mm (IH3.5 or IH4.0), and Morse taper 3.5 mm or 4.0 mm (MT3.5 or MT4.0). ...
Tópico(s): Dental Radiography and Imaging
2016 - Quintessence Publishing Company | The International Journal of Prosthodontics
Produção Nacional
Revisado por pares
Gileade Pereira Freitas, Helena Bacha Lopes, Evandro Carneiro Martins‐Neto, Paulo Tambasco de Oliveira, Márcio Mateus Beloti, Adalberto Luiz Rosa,
Clinical success of implant therapy is directly related to titanium (Ti) surface properties and the quality of bone tissue. The treatment of Ti implants with H2SO4/H2O2 is a feasible, reproducible, and low-cost technique to create surface nanotopography (Ti-Nano). As this nanotopography induces osteoblast differentiation, we hypothesized that it may affect bone response to Ti. Thus, this study was designed to evaluate the bone response to a machined Ti implant treated with H2SO4/H2O2 to generate Ti- ...
Tópico(s): Orthopaedic implants and arthroplasty
2015 - Allen Press | Journal of Oral Implantology
Produção Nacional
Revisado por pares
Emanuela Prado Ferraz, Alexander Tadeu Sverzut, Gileade Pereira Freitas, Juliana Carvalho Sá, Clodomiro Alves, Márcio Mateus Beloti, Adalberto Luiz Rosa,
A current goal of dental implant research is the development of titanium (Ti) surfaces to improve osseointegration. Plasma nitriding treatments generate surfaces that favor osteoblast differentiation, a key event to the process of osteogenesis. Based on this, it is possible to hypothesize that plasma-nitrided Ti implants may positively impact osseointegration. Objective The aim of this study was to evaluate the in vivo bone response to Ti surfaces modified by plasma-nitriding treatments. Material ...
Tópico(s): Dental Implant Techniques and Outcomes
2015 - UNIVERSIDADE DE SÃO PAULO | Journal of Applied Oral Science
Produção Nacional
Revisado por pares
Helena Bacha Lopes, Thiago de Santana Santos, Fabiola S de Oliveira, Gileade Pereira Freitas, Adriana LG de Almeida, Rossano Gimenes, Adalberto Luiz Rosa, Márcio Mateus Beloti,
In this study, we evaluated the effect of poly(vinylidene fluoride-trifluoroethylene)/barium titanate (P(VDF-TrFE)/BT) membrane on in vivo bone formation. Rat calvarial bone defects were implanted with P(VDF-TrFE)/BT and polytetrafluoroethylene (PTFE) membranes, and at 4 and 8 weeks, histomorphometric and gene expression analyses were performed. A higher amount of bone formation was noticed on P(VDF-TrFE)/BT compared with PTFE. The gene expression of RUNX2, bone sialoprotein, osteocalcin, receptor ...
Tópico(s): Bone and Dental Protein Studies
2013 - SAGE Publishing | Journal of Biomaterials Applications
Steven Coutré, John P. Leonard, Richard R. Furman, Jacqueline C. Barrientos, Sven de Vos, Ian W. Flinn, Marshall T. Schreeder, Nina D. Wagner‐Johnston, Jeff P. Sharman, Thomas E. Boyd, Nathan Fowler, Leanne Holes, Brian J. Lannutti, Dave M. Johnson, Langdon L. Miller, Thomas M. Jahn,
... GS-1101 (CAL-101)-based combination therapy. Leonard:Gileade: Consultancy. Barrientos:Gilead: Research Funding. de Vos:Gilead: ...
Tópico(s): Lymphoma Diagnosis and Treatment
2012 - Elsevier BV | Blood
Produção Nacional
Revisado por pares
Alann Thaffarell Portilho Souza, Helena Bacha Lopes, Gileade Pereira Freitas, Emanuela Prado Ferraz, Fabíola Singaretti Oliveira, Adriana Luisa Gonçalves Almeida, Denise Weffort, Márcio Mateus Beloti, Adalberto Luiz Rosa,
Tópico(s): Mesenchymal stem cell research
2020 - Springer Science+Business Media | Journal of Bone and Mineral Metabolism
Produção Nacional
Revisado por pares
Doo‐Yeol Yoo, Charles Marin, Gileade Pereira Freitas, Nick Tovar, Estevam A. Bonfante, Hellen S. Teixeira, Malvin N. Janal, Paulo G. Coelho,
Dual acid-etching is widely used to modify dental implant topography and enhance early bone healing. This study evaluated the histomorphometric, biomechanical, and histological bone response to acid-etched (AA) in comparison with grit-blasted/acid-etched (GB) and machined control (C) implants within sites of relatively low-bone remodeling rates.Implant surface topography was evaluated by scanning electron microscopy and optical interferometry (IFM). Six adult male sheep (n = 6) received 72 Ti-6Al-4V implants ( ...
Tópico(s): Orthopaedic implants and arthroplasty
2015 - Lippincott Williams & Wilkins | Implant Dentistry
Produção Nacional
Revisado por pares
Ronaldo Hirata, Estevam A. Bonfante, Rodolfo Bruniera Anchieta, Lucas Silveira Machado, Gileade Pereira Freitas, Vinícius P. Fardin, Nick Tovar, Paulo G. Coelho,
Narrow implants are indicated in areas of limited bone width or when grafting is nonviable. However, the reduction of implant diameter may compromise their performance. This study evaluated the reliability of several narrow implant systems under fatigue, after restored with single-unit crowns.Narrow implant systems were divided (n = 18 each), as follows: Astra (ASC); BioHorizons (BSC); Straumann Roxolid (SNC), Intra-Lock (IMC), and Intra-Lock one-piece abutment (ILO). Maxillary central incisor crowns were ...
Tópico(s): Bone Tissue Engineering Materials
2015 - Springer Nature | Clinical Oral Investigations
Produção Nacional
Revisado por pares
Letícia Faustino Adolpho, Helena Bacha Lopes, Gileade Pereira Freitas, Denise Weffort, Gabriela Guaraldo Campos Totoli, Ana Carolina Loyola Barbosa, Rahyza Inácio Freire de Assis, Karina Gonzáles Silvério, Denise Carleto Andia, Adalberto Luiz Rosa, Márcio Mateus Beloti,
Aim: This study aimed to evaluate the ability of human periodontal ligament stem cells (PDLSCs) with high (HP-PDLSCs) and low (LP-PDLSCs) osteogenic potential, in addition to mixed cells, to repair bone tissue. Methods: Cell phenotype, proliferation and differentiation were evaluated. Undifferentiated PDLSCs were injected into rat calvarial defects and the new bone was evaluated by μCT, histology and real-time PCR. Results: PDLSCs exhibited a typical mesenchymal stem cell phenotype and HP-PDLSCs showed ...
Tópico(s): dental development and anomalies
2022 - Future Medicine | Regenerative Medicine
Produção Nacional
Revisado por pares
Thaís Moré Milan, Ana Patrícia Espaladori Eskenazi, Lucas Dias de Oliveira, Gabriel Silva, Rayana Longo Bighetti‐Trevisan, Gileade Pereira Freitas, Luciana O. Almeida,
The Wnt/β-catenin signaling pathway is associated with the regulation of cancer stem cells, and it can be driven by epigenetic modifications. Here, we aim to identify epigenetic modifications involved in the control of the Wnt/β-catenin signaling and investigate the role of this pathway in the accumulation of cancer stem cells (CSC) and chemoresistance of Head and Neck Squamous Cell Carcinoma (HNSCC). Quantitative-PCR, western blot, shRNA assay, viability assay, flow cytometry assay, spheres formation, ...
Tópico(s): Cancer-related gene regulation
2023 - Elsevier BV | Cellular Signalling
Produção Nacional
Gustavo Antônio Corrêa Momesso, Tárik Ocon Braga Polo, William Phillip Pereira da Silva, Stéfany Barbosa, Gileade Pereira Freitas, Helena Bacha Lopes, Adalberto Luiz Rosa, Jairo M. Cordeiro, Luan Felipe Toro, Fernando Yamamoto Chiba, Dóris Hissako Matsushita, Mário Jefferson Quirino Louzada, Nilson Cristino da Cruz, Valentim Adelino Ricardo Barão, Leonardo Pérez Faverani,
The treatment of polytrauma patients represents a great challenge in the maxillofacial and orthopedic surgery fields. Therefore, this study tested the hypothesis that the use of a bioactive coating (by plasma electrolytic oxidation, PEO) on titanium microplates could improve the fracture healing of low bone mineral density (BMD) rats. Thirty female rats underwent bilateral ovariectomy surgery (OVX), and 35 rats underwent fake surgery (SHAM). Three months later, animals were subjected to femoral fracture ...
Tópico(s): Hip and Femur Fractures
2020 - Elsevier BV | Materials Science and Engineering C
Produção Nacional
Revisado por pares
Alann Thaffarell Portilho Souza, Gileade Pereira Freitas, Helena Bacha Lopes, Denise Weffort, Letícia Faustino Adolpho, Maria Paula Oliveira Gomes, Fabíola Singaretti Oliveira, Adriana Luisa Gonçalves Almeida, Márcio Mateus Beloti, Adalberto Luiz Rosa,
Cell therapy utilizing mesenchymal stem cells (MSCs) from healthy donors (HE-MSCs) is a promising strategy for treating osteoporotic bone defects. This study investigated the effects of interaction between HE-MSCs and MSCs from osteoporotic donors (ORX-MSCs) on osteoblast differentiation of MSCs and of HE-MSCs on bone formation in calvarial defects of osteoporotic rats. Osteoporosis was induced by orchiectomy (ORX) and its effects on the bone were evaluated by femur microtomography (μCT) and osteoblast ...
Tópico(s): Bone Metabolism and Diseases
2024 - Elsevier BV | Life Sciences
Produção Nacional
Revisado por pares
Adriana Luisa Gonçalves Almeida, Gileade Pereira Freitas, Helena Bacha Lopes, Rossano Gimenes, Selma Siéssere, Luiz Gustavo de Sousa, Márcio Mateus Beloti, Adalberto Luiz Rosa,
Cell therapy associated with guided bone regeneration (GBR) can be used to treat bone defects under challenging conditions such as osteoporosis. This study aimed to evaluate the effect of mesenchymal stem cells (MSCs) in combination with a poly(vinylidene-trifluoroethylene)/barium titanate (PVDF-TrFE/BT) membrane on bone repair in osteoporotic rats. Osteoporosis was induced in female rats by bilateral removal of the ovaries (OVX) or sham surgery (SHAM), and the osteoporotic condition was characterized ...
Tópico(s): Orthopaedic implants and arthroplasty
2019 - Brazilian Society of Dental Research | Brazilian Oral Research
Carla Viana Dendasck, Gileade Ferreira Lopes,
O comportamento humano é algo fascinante e ao mesmo tempo enigmático, sendo ele um objeto de estudo de diversas áreas do conhecimento, dentre elas a psicanálise, a psicologia, a antropologia e a sociologia. O presente artigo se trata de uma pesquisa qualitativa exploratória e tem como objetivos a compreensão de um conceito rico para as ciências sociais a partir do pensamento de dois importantes autores, Norbert Elias e Pierre Bourdieu. O estudo foi realizado através de uma pesquisa bibliográfica ...
Tópico(s): Race, Identity, and Education in Brazil
2016 - | Revista Científica Multidisciplinar Núcleo do Conhecimento
Produção Nacional
Revisado por pares
Arpita Patel, Luiz Fernando Gil, Arthur Castellano, Gileade Pereira Freitas, Daniel Navarro, Ana P. Peredo, Nick Tovar, Paulo G. Coelho,
This study was designed to compare the combined effect of two different drilling techniques (conventional expansion and one-step) and four different implant geometries in a beagle dog model. The nondecalcified bone-implant samples underwent histologic/metric analysis at 2 and 6 weeks. Morphologic analysis showed similarities between different drilling technique groups and implant geometries. Histomorphometric parameters, bone-to-implant contact (BIC), and bone area fraction occupancy (BAFO) were analyzed, ...
Tópico(s): Orthopedic Surgery and Rehabilitation
2016 - Quintessence Publishing Company | The International Journal of Periodontics & Restorative Dentistry
Produção Nacional
Revisado por pares
Gabriela Guaraldo Campos Totoli, Rayana Longo Bighetti‐Trevisan, Gileade Pereira Freitas, Letícia Faustino Adolpho, Adriana Luisa Golçalves Almeida, Ana Carolina Loyola Barbosa, Jaqueline Isadora Reis Ramos, Márcio Mateus Beloti, Adalberto Luiz Rosa,
Aim: We evaluated the bone repair induced by MSCs from adipose tissue (AT-MSCs) and bone marrow (BM-MSCs) injected into rat calvarial defects at two time points. Methods & results: Both cell populations expressed MSC surface markers and differentiated into adipocytes and osteoblasts. μCT showed that the combination of cells from distinct sources exhibited synergistic effects to increase bone repair with an advantage when BM-MSCs were injected prior to AT-MSCs. The higher osteogenic potential of these ...
Tópico(s): Bone Tissue Engineering Materials
2023 - Future Medicine | Regenerative Medicine
Produção Nacional
Ivan Ricardo Carvalho, Luiz Leonardo Ferreira, Giordano Gelain Conte, Gileade Costa Leonel Amaral, Juliana Naves de Campos, Augusto Antonio Souza Tomazele, Núbia Sousa Carrijo, Valúcia Teodoro Pereira, Adriano Teixeira Souza, Murilo Vieira Loro,
This study aimed to evaluate the effect of biostimulant positioning on common bean cultivars in the southwestern Goiás conditions. The experiment was conducted in the municipality of Mineiros-GO. The experimental design was a randomized block in factorial 6x3, corresponding to the positioning of the Triplus® biostimulator (R5, R5 + R8 and water) in six common bean cultivars (BRS Campeiro, BRS Esteio, BRS Estilo, BRS Marfim, BRS Notavel and BRS Perola) in 4 replicates. The following productive attributes ...
Tópico(s): Seed Germination and Physiology
2021 - | Agronomy Science and Biotechnology