Research Article| March 01 1980 Verdi, Giuseppina Pasqua, and the Composition of "Falstaff" James A. Hepokoski James A. Hepokoski Search for other works by this author on: This Site PubMed ... Cite Search Site Citation James A. Hepokoski; Verdi, Giuseppina Pasqua, and the Composition of "Falstaff". 19th-Century Music ...
Tópico(s): Music Technology and Sound Studies
1980 - University of California Press | 19th-Century Music
Tópico(s): Music Technology and Sound Studies
1980 - University of California Press | 19th-Century Music
Pasqua Anna Quitadamo, Giuseppina Palumbo, Liliana Cianti, Paola Lurdo, Maria Assunta Gentile, Antonio Villani,
The review recalls the importance of breast milk and deepens the theme of human milk banking, a virtuous reality that is expanding all over the world but is still little known. In the last 15 years, modern biological technologies have crystallized the concept of uniqueness and irreproducibility of human milk, by establishing three new principles: first: human milk: a “life-saving” drug; second: human milk: the best food for preterm infants; and third: human milk: the main component of premature infant ...
Tópico(s): Neonatal Respiratory Health Research
2021 - Hindawi Publishing Corporation | International Journal of Pediatrics
Pasqua Anna Quitadamo, Giuseppina Palumbo, Liliana Cianti, Matteo Luigi Napolitano, Ciro Coviello, Paola Lurdo, Massimiliano Copetti, Maria Assunta Gentile, Pierpaolo Cristalli,
The breast milk is the gold standard food for the feeding of the premature baby: it is the natural way to provide excellent nutritional, immunological, and biological nutriment so as to facilitate a healthy growth and the development of the infants. When the breast milk is not available, the alternative is represented by the donated milk. The mothers of premature infants are important opportunity if we consider the fact that they could devote some milk both because they provide a food which is closer ...
Tópico(s): Child Nutrition and Water Access
2018 - Hindawi Publishing Corporation | International Journal of Pediatrics
Laura Giuseppina Di Pasqua, Marta Cagna, Clarissa Berardo, Mariapia Vairetti, Andrea Ferrigno,
Non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) are some of the biggest public health challenges due to their spread and increasing incidence around the world. NAFLD is characterized by intrahepatic lipid deposition, accompanied by dyslipidemia, hypertension, and insulin resistance, leading to more serious complications. Among the various causes, drug administration for the treatment of numerous kinds of diseases, such as antiarrhythmic and antihypertensive drugs, ...
Tópico(s): Hepatitis C virus research
2022 - Multidisciplinary Digital Publishing Institute | Biomedicines
Mariapia Vairetti, Laura Giuseppina Di Pasqua, Marta Cagna, Plinio Richelmi, Andrea Ferrigno, Clarissa Berardo,
Glutathione (GSH), a tripeptide particularly concentrated in the liver, is the most important thiol reducing agent involved in the modulation of redox processes. It has also been demonstrated that GSH cannot be considered only as a mere free radical scavenger but that it takes part in the network governing the choice between survival, necrosis and apoptosis as well as in altering the function of signal transduction and transcription factor molecules. The purpose of the present review is to provide ...
Tópico(s): Genomics, phytochemicals, and oxidative stress
2021 - Multidisciplinary Digital Publishing Institute | Antioxidants
Clarissa Berardo, Laura Giuseppina Di Pasqua, Marta Cagna, Plinio Richelmi, Mariapia Vairetti, Andrea Ferrigno,
Nonalcoholic fatty liver disease (NAFLD) is a continuum of liver abnormalities often starting as simple steatosis and to potentially progress into nonalcoholic steatohepatitis (NASH), fibrosis, cirrhosis and hepatocellular carcinoma. Because of its increasing prevalence, NAFLD is becoming a major public health concern, in parallel with a worldwide increase in the recurrence rate of diabetes and metabolic syndrome. It has been estimated that NASH cirrhosis may surpass viral hepatitis C and become the ...
Tópico(s): Endoplasmic Reticulum Stress and Disease
2020 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
G. Palladini, Laura Giuseppina Di Pasqua, Clarissa Berardo, Veronica Siciliano, Plinio Richelmi, Stefano Perlini, Andrea Ferrigno, Mariapia Vairetti,
Animal models of obstructive cholestasis and ischemia/reperfusion damage have revealed the functional heterogeneity of liver lobes. This study evaluates this heterogeneity in nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) rat models. Twelve-week-old Obese and Lean male Zucker rats were used for NAFLD. Eight-week-old male Wistar rats fed with 8-week methionine-choline-deficient (MCD) diet and relative control diet were used for NASH. Gelatinase (MMP-2; MMP-9) activity ...
Tópico(s): Liver Diseases and Immunity
2019 - Pulsus Group | Canadian Journal of Gastroenterology and Hepatology
Clarissa Berardo, Veronica Siciliano, Laura Giuseppina Di Pasqua, Plinio Richelmi, Mariapia Vairetti, Andrea Ferrigno,
RNA interference is a powerful approach to understand gene function both for therapeutic and experimental purposes. Since the lack of knowledge in the gene silencing of various hepatic cell lines, this work was aimed to compare two transfection agents, the liposome-based Lipofectamine™ RNAiMAX and the HepG2-specific, polymer-based GenMute™, in two cellular models of human hepatoma, HepG2 and Huh7.5. In the first part, we assessed transfection efficiency of a fluorescent Cy3-labeled negative control ...
Tópico(s): Virus-based gene therapy research
2019 - PAGEPress (Italy) | European Journal of Histochemistry
G. Palladini, Laura Giuseppina Di Pasqua, Clarissa Berardo, Veronica Siciliano, Plinio Richelmi, Barbara Mannucci, Anna Cleta Croce, Vittoria Rizzo, Stefano Perlini, Mariapia Vairetti, Andrea Ferrigno,
We investigated changes in fatty acid desaturases, D5D, D6D, D9-16D and D9-18D, and their relationship with oxidative stress, matrix metalloproteinases (MMPs) and serum TNF-alpha in two rat models of non-alcoholic fatty liver disease NAFLD. Eight-week-old male Wistar rats fed for 3 weeks with methionine-choline–deficient (MCD) diet and eleven-week-old Obese male Zucker rats were used. Serum levels of hepatic enzymes and TNF-alpha were quantified. Hepatic oxidative stress (ROS, TBARS and GSH content) ...
Tópico(s): Lipid metabolism and biosynthesis
2019 - Multidisciplinary Digital Publishing Institute | Nutrients
Andrea Ferrigno, Clarissa Berardo, Laura Giuseppina Di Pasqua, Veronica Siciliano, Plinio Richelmi, Ferdinando Nicoletti, Mariapia Vairetti,
2-Methyl-6-(phenylethynyl)pyridine (MPEP), a negative allosteric modulator of the metabotropic glutamate receptor (mGluR) 5, protects hepatocytes from ischemic injury. In astrocytes and microglia, MPEP depletes ATP. These findings seem to be self-contradictory, since ATP depletion is a fundamental stressor in ischemia. This study attempted to reconstruct the mechanism of MPEP-mediated ATP depletion and the consequences of ATP depletion on protection against ischemic injury. We compared the effects ...
Tópico(s): Organ Transplantation Techniques and Outcomes
2018 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Andrea Ferrigno, Clarissa Berardo, Laura Giuseppina Di Pasqua, Veronica Siciliano, Plinio Richelmi, Mariapia Vairetti,
and mGluR5 knockout mice are protected against ischemic injury.Similar results have been observed in experimental models of free-radical injury and in vivo mouse models of acetaminophen intoxication.Finally, mGluR5s in the pancreas are associated with insulin secretion control.The picture is, however, far from complete as the review attempts to establish in particular as regards identifying specific targets and innovative therapeutic approaches for the treatment of GI disorders.
Tópico(s): Biochemical Analysis and Sensing Techniques
2017 - Baishideng Publishing Group | World Journal of Gastroenterology
Anna Cleta Croce, Andrea Ferrigno, Vittorio Bertone, Valeria Maria Piccolini, Clarissa Berardo, Laura Giuseppina Di Pasqua, Vittoria Rizzo, Giovanni Bottiroli, Mariapia Vairetti,
Livers with moderate steatosis are currently recruited as marginal organs to face donor shortage in transplantation, even though lipid excess and oxidative stress increase preservation injury risk. Sensitive, real-time detection of liver metabolism engagement could help donor selection and preservation procedures, ameliorating the graft outcome. Hence, we investigated endogenous biomolecules with autofluorescence (AF) properties as biomarkers supporting the detection of liver oxidative events and ...
Tópico(s): Liver Disease Diagnosis and Treatment
2016 - Wiley | Hepatology Research
Andrea Ferrigno, G. Palladini, Alberto Bianchi, Vittoria Rizzo, Laura Giuseppina Di Pasqua, Stefano Perlini, Plinio Richelmi, Mariapia Vairetti,
We investigated the effects of obstructive cholestasis in different hepatic lobes by evaluating asymmetric dimethylarginine (ADMA) (a nitric oxide synthase inhibitor), protein methyltransferase (PRMT) and dimethylarginine dimethylaminohydrolase (DDAH) (enzymes involved, resp., in its synthesis and degradation), the cationic transporter (CAT), and metalloproteinase (MMP) activity. Sixteen male Wistar rats underwent a 3-day cholestasis by common bile duct ligation (BDL) or sham operation. Blood samples and ...
Tópico(s): Pharmacological Effects and Toxicity Studies
2014 - Hindawi Publishing Corporation | BioMed Research International
Ilaria Canobbio, Silvia Catricalà, Laura Giuseppina Di Pasqua, Gianni Francesco Guidetti, Alessandra Consonni, Daria Manganaro, Mauro Torti,
Accumulation of amyloidogenic Aβ peptides in the brain contributes to the onset of Alzheimer disease. Aβ peptide deposits are also present in blood vessel walls, mainly deriving from circulating platelets. However, their effect on platelet function is unclear. We demonstrate that immobilized Aβ peptides induce platelet adhesion and spreading through metalloproteinase‐sensitive surface receptors. Aβ peptides also fasten platelet spreading on collagen, and support the time‐ and ADP‐dependent activation ...
Tópico(s): Diabetes Treatment and Management
2013 - Wiley | FEBS Letters
G. Palladini, Laura Giuseppina Di Pasqua, Marta Cagna, Anna Cleta Croce, Stefano Perlini, Barbara Mannucci, Antonella Profumo, Andrea Ferrigno, Mariapia Vairetti,
We evaluate the effects of the methionine-choline-deficient (MCD) diet on serum and hepatic zinc (Zn) and iron (Fe) and their relationships with matrix metalloproteinases (MMPs) and their modulators (TIMPs and RECK) as well as hepatic fatty acids using male Wistar rats fed 2-, 4- and 8-week MCD diets. Serum and hepatic Zn decrease after an 8-week MCD diet. Serum Fe increases after an 8-week MCD diet and the same occurs for hepatic Fe. An increase in hepatic MMP activity, associated with a decrease in ...
Tópico(s): Folate and B Vitamins Research
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Andrea Ferrigno, Laura Giuseppina Di Pasqua, Clarissa Berardo, Veronica Siciliano, Vittoria Rizzo, Barbara Mannucci, Plinio Richelmi, Anna Cleta Croce, Mariapia Vairetti,
We compared static preservation (cold storage, CS, 4 °C) with dynamic preservation (machine perfusion, MP, 20 °C) followed by reperfusion using marginal livers: a model of donation after cardiac death (DCD) livers and two models of fatty livers, the methionine-choline deficient (MCD) diet model, and obese Zucker (fa/fa) rats. CS injury in DCD livers was reversed by an oxygenated washout (OW): hepatic damage, bile flow, and the ATP/ADP ratio in the OW + CS group was comparable with the ratio obtained with ...
Tópico(s): Cardiac Ischemia and Reperfusion
2017 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Anna Cleta Croce, Andrea Ferrigno, Laura Giuseppina Di Pasqua, Clarissa Berardo, Valeria Maria Piccolini, Vittorio Bertone, Giovanni Bottiroli, Mariapia Vairetti,
Liver tissue autofluorescence (AF) has been characterized in two models with a different potential to undergo disease progression to steatohepatitis: Wistar rats, administered with a methionine, choline deficient diet (MCD), and Zucker (fa/fa) rats, homozygous for a spontaneous mutation of leptin receptor. AF spectra were recorded from liver tissue cryostatic sections by microspectrofluorometry, under 366 nm excitation. Curve fitting analysis was used to estimate the contribution of different endogenous ...
Tópico(s): Liver Disease Diagnosis and Treatment
2016 - Elsevier BV | Journal of Photochemistry and Photobiology B Biology
Andrea Ferrigno, Vittoria Rizzo, Alberto Bianchi, Laura Giuseppina Di Pasqua, Clarissa Berardo, Plinio Richelmi, Mariapia Vairetti,
We investigated the effects of hepatic ischemia/reperfusion (I/R) injury on asymmetric dimethylarginine (ADMA, a nitric oxide synthase inhibitor), protein methyltransferase (PRMT) and dimethylarginine dimethylaminohydrolase (DDAH) (involved, resp., in ADMA synthesis and degradation), and the cationic transporter (CAT). Male Wistar rats were subjected to 30 or 60 min hepatic ischemia followed by 60 min reperfusion. ADMA levels in serum and bile were determined. Tissue ADMA, DDAH activity, DDAH-1 and CAT- ...
Tópico(s): Eicosanoids and Hypertension Pharmacology
2014 - Hindawi Publishing Corporation | BioMed Research International
Andrea Ferrigno, Laura Giuseppina Di Pasqua, Alberto Bianchi, Plinio Richelmi, Mariapia Vairetti,
AIM:To study at what temperature the oxygen carried by the perfusate meets liver requirements in a model of organ perfusion.METHODS: in this study, we correlated hypoxia inducible factor (HiF)-1α expression to the perfusion temperature and the hepatic oxygen uptake in a model of isolated perfused rat liver.Livers from Wistar rats were perfused for 6 h with an oxygenated medium at 10, 20, 30 and 37 ℃.Oxygen uptake was measured by an oxygen probe; lactate dehydrogenase activity, lactate release and glycogen ...
Tópico(s): Liver Disease Diagnosis and Treatment
2015 - Baishideng Publishing Group | World Journal of Gastroenterology
Laura Giuseppina Di Pasqua, Marta Cagna, G. Palladini, Anna Cleta Croce, Massimiliano Cadamuro, Luca Fabris, Stefano Perlini, Luciano Adorini, Andrea Ferrigno, Mariapia Vairetti,
Abstract Background and Aims We have previously shown in a model of hepatic ischaemia/reperfusion injury that the farnesoid X receptor (FXR) agonist obeticholic acid (OCA) restores reversion‐inducing‐cysteine‐rich protein with Kazal motifs (RECK), an inverse modulator of metalloproteases (MMPs) and inhibitor of the sheddases ADAM10 and ADAM17 involved in inflammation and fibrogenesis. Here, the effects of FXR agonists OCA and INT‐787 on hepatic levels of RECK, MMPs, ADAM10 and ADAM17 were compared in ...
Tópico(s): Liver Diseases and Immunity
2023 - Wiley | Liver International
Andrea Ferrigno, Clarissa Berardo, Laura Giuseppina Di Pasqua, Marta Cagna, Veronica Siciliano, Plinio Richelmi, Mariapia Vairetti,
It has been previously found that the blockade of metabotropic glutamate receptor type 5 (mGluR5) protects against hepatic ischemia/reperfusion injury and acetaminophen toxicity. The role of mGluR5 in NAFLD has not yet been elucidated. Here, we evaluated the effects of mGluR5 blockade in an in vitro model of steatosis. HepG2 cells were pre-incubated for 12 h with an mGluR5 agonist, a negative allosteric modulator (DHPG and MPEP, respectively) or vehicle, then treated with 1.5 mM oleate/palmitate (O/ ...
Tópico(s): Adipose Tissue and Metabolism
2020 - PAGEPress (Italy) | European Journal of Histochemistry
Laura Giuseppina Di Pasqua, Clarissa Berardo, Vittoria Rizzo, Plinio Richelmi, Anna Cleta Croce, Mariapia Vairetti, Andrea Ferrigno,
Tópico(s): Peroxisome Proliferator-Activated Receptors
2016 - Springer Science+Business Media | Molecular and Cellular Biochemistry
Pasqua Anna Quitadamo, Laura Comegna, Giuseppina Palumbo, Massimiliano Copetti, Paola Lurdo, Federica Zambianco, Maria Assunta Gentile, Antonio Villani,
Background: Over the past year, there has been a rise in twin births. The current scientific consensus recommended breast-feed milk for all newborns for at least 6 months. They stated that it is possible to meet the nutritional needs of two or more newborns with only one mother’s milk. More information would be desirable about the factors that influence or lead to the initiation and interruption of breastfeeding. The quality of the evidence available from multiple studies has been inconclusive and ...
Tópico(s): Child Nutrition and Feeding Issues
2021 - Multidisciplinary Digital Publishing Institute | Nutrients
Andrea Ferrigno, Laura Giuseppina Di Pasqua, G. Palladini, Clarissa Berardo, Roberta Verta, Plinio Richelmi, Stefano Perlini, Debora Collotta, Massimo Collino, Mariapia Vairetti,
In this study, we demonstrated the involvement of matrix metalloproteinases (MMPs) in hepatic ischemia/reperfusion (I/R) injury. Our aim is to evaluate the impact of reperfusion on I/R-related changes in RECK, an MMP modulator, and mitogen-activated protein kinase (MAPKs) pathways (ERK, p38, and JNK). Male Wistar rats were either subjected to 60 min partial-hepatic ischemia or sham-operated. After a 60 min or 120 min reperfusion, liver samples were collected for analysis of MMP-2 and MMP-9 by zymography ...
Tópico(s): Liver Disease and Transplantation
2020 - Multidisciplinary Digital Publishing Institute | Biomolecules
Andrea Ferrigno, Laura Giuseppina Di Pasqua, Clarissa Berardo, Veronica Siciliano, Vittoria Rizzo, Luciano Adorini, Plinio Richelmi, Mariapia Vairetti,
Background We previously showed that increased asymmetric dimethylarginine (ADMA) biliary excretion occurs during hepatic ischemia/reperfusion (I/R), prompting us to study the effects of the farnesoid X receptor (FXR) agonist obeticholic acid (OCA) on bile, serum and tissue levels of ADMA after I/R. Material and methods Male Wistar rats were orally administered 10mg/kg/day of OCA or vehicle for 5 days and were subjected to 60 min partial hepatic ischemia or sham-operated. After a 60 min reperfusion, ...
Tópico(s): Liver Disease and Transplantation
2018 - Public Library of Science | PLoS ONE
Anna Cleta Croce, Andrea Ferrigno, Clarissa Berardo, Giovanni Bottiroli, Mariapia Vairetti, Laura Giuseppina Di Pasqua,
Autofluorescence (AF) of crude serum was investigated with reference to the potential of its intrinsic AF biomarkers for the noninvasive diagnosis of liver injury. Spectral parameters of pure compounds representing retinol (vitamin A) and fluorescing free fatty acids were characterized by spectrofluorometry, to assess spectral parameters for the subsequent AF analysis of serum, collected from rats undergoing liver ischemia/reperfusion (I/R). Differences in AF spectral profiles detected between control ...
Tópico(s): Eicosanoids and Hypertension Pharmacology
2020 - Multidisciplinary Digital Publishing Institute | Molecules
Andrea Ferrigno, G. Palladini, Laura Giuseppina Di Pasqua, Clarissa Berardo, Plinio Richelmi, Massimiliano Cadamuro, Luca Fabris, Stefano Perlini, Luciano Adorini, Mariapia Vairetti,
Background We have previously shown that obeticholic acid (OCA) upregulates the biliary excretion of asymmetric dimethylarginine (ADMA), an inhibitor of iNOS regulating the activity of matrix metalloproteinases (MMPs). Here, the effects of OCA on MMP-2 and MMP-9 activity in liver, bile and serum were evaluated after hepatic ischemia/reperfusion (I/R) injury. Material and methods Male Wistar rats (n = 20) were orally administered 10 mg/kg/day of OCA (5 days) and subjected to a 60-min ischemia and 60-min ...
Tópico(s): Pediatric Hepatobiliary Diseases and Treatments
2020 - Public Library of Science | PLoS ONE
Clarissa Berardo, Laura Giuseppina Di Pasqua, Veronica Siciliano, Vittoria Rizzo, Plinio Richelmi, Andrea Ferrigno, Mariapia Vairetti,
BACKGROUND:Ischemic cholangitis is the main cause of liver failure after transplantation and subnormothermic machine perfusion may represent a better strategy than conventional cold storage, minimizing preservation injury. We compared livers preserved by machine perfusion at 20°C (MP20) or by cold storage at 4°C (CS4) with regard to hypoxia-inducible factor (HIF)-1α mRNA expression and protein stabilization in hypoxic conditions. MATERIAL AND METHODS:Livers from male Wistar rats were stored on ice ...
Tópico(s): Hydrogen's biological and therapeutic effects
2017 - International Scientific Information Inc. | Annals of Transplantation
Andrea Ferrigno, Laura Giuseppina Di Pasqua, Clarissa Berardo, Veronica Siciliano, Plinio Richelmi, Mariapia Vairetti,
The role of Na+ in hypoxic injury was evaluated by a time-course analysis of damage in isolated livers perfused with N2-saturated buffer containing standard (143 mM) or low (25 mM) Na+ levels. Trypan blue uptake was used to detect non-viable cells. Under hypoxia with standard-Na+, trypan blue uptake began at the border between pericentral areas and periportal regions and increased in the latter zone; using a low-Na+ buffer, no trypan blue zonation occurred but a homogenous distribution of dye was ...
Tópico(s): Eicosanoids and Hypertension Pharmacology
2017 - PAGEPress (Italy) | European Journal of Histochemistry