M. Morais‐Almeida, Angéla Gáspár, Graça Pires, Sara Prates, José Rosado‐Pinto,
Childhood recurrent wheezing is a very prevalent heterogeneous clinical entity. An 8-year prospective study was performed to correlate the clinical outcome of recurrent wheezing in the first years of life with prognostic risk factors. A cohort of 308 children with recurrent wheezing, aged <7 years, were enrolled in 1993, studied using a questionnaire, skin-prick tests, and serum total IgE. According to the study protocol, in 1996 and 2001, the cohort was assessed. In 2001, 81% of the initial sample ...
Tópico(s): Neonatal Respiratory Health Research
2007 - OceanSide Publications, Inc | Allergy and Asthma Proceedings
António Travassos, Susana Teixeira, Pinto Ferreira, Isaura Regadas, Ana Sofia Marques Travassos, F Esperancinha, Isabel Prieto, Graça Pires, Robert van Velze, António Marques Valido, Maria do Céu Machado,
<P>The anatomic response to intravitreal bevacizumab injection in three patients with aggressive, posterior retinopathy of prematurity is described. In all cases, the worse eye was treated with a single intravitreal injection of 0.75 mg of bevacizumab as monotherapy or complementary to laser therapy. In 24 hours, all injected eyes showed regression of the tunica vasculosa lentis and iris vessel engorgement and disappearance of iris rigidity. In addition, plus disease and retinal proliferation ...
Tópico(s): Retinal and Macular Surgery
2007 - | Ophthalmic surgery, lasers & imaging retina
Inês Mota, Angéla Gáspár, Filipe Benito-Garcia, Magna Alves‐Correia, Cristina Arêde, Susana Piedade, Graça Sampaio, Graça Pires, Cristina Santa-Marta, Luís Miguel Borrego, Mário Morais‐Almeida,
Lipid transfer proteins (LTPs) are panallergens found in many plant foods. They are a common cause of food-induced anaphylaxis (FIA) in adults living in the Mediterranean area. LTPs have also been proposed as a main cause of food-dependent exercise-induced anaphylaxis (FDEIA).Describe clinical characteristics and allergen sensitization profiles in patients with FIA related to LTP.Forty-three patients were included, aged 3-52 years with a clinical history of FIA and proven sensitization to LTP. Patients ...
Tópico(s): Contact Dermatitis and Allergies
2018 - Elsevier BV | Allergologia et Immunopathologia
Maria Rosa Rebordão, Carla Carneiro, Graça Alexandre‐Pires, Patrícia H. Brito, Catarina Pereira, Telmo Nunes, António Galvão, Alexandre Leitão, Camila Vilela, Graça Ferreira‐Dias,
Besides the classical functions, neutrophils (PMNs) are able to release DNA in response to infectious stimuli, forming neutrophil extracellular traps (NETs) and killing pathogens. The pathogenesis of endometritis in the mare is not completely understood. The aim was to evaluate the in vitro capacity of equine PMNs to secrete NETs by chemical activation, or stimulated with Streptococcus equi subspecies zooepidemicus (Szoo), Escherichia coli (Ecoli) or Staphylococcus capitis (Scap) strains obtained from ...
Tópico(s): Cell Adhesion Molecules Research
2014 - Elsevier BV | Journal of Reproductive Immunology
Luísa Mendes-Jorge, David Ramos‐Barbón, Mariana Luppo, Cristina Llombart, Graça Alexandre‐Pires, Víctor Nacher, Verònica Melgarejo, Miguel Correia, Marc Navarro, Ana Carretero, Sabrina Tafuro, Alfonso Rodríguez‐Baeza, José António Esperança-Pina, Fàtima Bosch, Jesús Ruberte,
Purpose.: The retina contains two distinct populations of monocyte-derived cells: perivascular macrophages, and microglia. The present study was undertaken to evaluate the presence and function in mouse and human retinas of a subtype of resident perivascular macrophages with scavenger function, different from microglia, in physiological conditions and during retinopathy. Methods.: Perivascular macrophages were characterized by means of confocal microscopy, electron microscopy, and flow cytometry ...
Tópico(s): Glaucoma and retinal disorders
2009 - Cadmus Press | Investigative Ophthalmology & Visual Science
Graça Alexandre‐Pires, Maria Teresa Villa de Brito, Cármen Algueró, Catarina Martins, Olı́via Roos Rodrigues, Isabel Pereira da Fonseca, Gabriela Santos‐Gomes,
Canine visceral leishmaniasis (CanL) is an emerging disease, expanding in various parts of the world. The infection caused by Leishmania, an intracellular protozoan parasite, can show different clinical manifestations, from asymptomatic or subclinical to symptomatic dogs, in which a wide spectrum of clinical signs is evident. The fact that the parasite replicates in different organs raises the hypothesis that each organ may have a specific immune response. The local immune responses should be evaluated ...
Tópico(s): Toxin Mechanisms and Immunotoxins
2010 - Elsevier BV | Veterinary Immunology and Immunopathology
Produção Nacional
Revisado por pares
Marco Antônio Granja Barbosa, Graça Alexandre‐Pires, Marta Soares-Clemente, Cláudia Marques, Olı́via Roos Rodrigues, Teresa Villa de Brito, Isabel Pereira da Fonseca, Lêucio Câmara Alves, Gabriela Santos‐Gomes,
Canine leishmaniosis (CanL) caused by the protozoan parasite Leishmania infantum is a chronic systemic disease that is endemic in certain parts of the world. The domestic dog is the most important reservoir of L. infantum and is the main source of infection for other animals and for the human population. The aim of this study was to evaluate and compare the level of expression of genes encoding particular cytokines (interleukin [IL]-12, interferon [IFN]-γ, IL-2 and IL-4) in different tissues and organs ...
Tópico(s): Leptospirosis research and findings
2011 - Elsevier BV | Journal of Comparative Pathology
Graça Alexandre‐Pires, Diogo Pais, Maria João Correia, J. A. Esperança Pina,
Leishmaniosis is an anthropozoonosis caused by an intracellular protozoan parasite that causes a wide spectrum of diseases in humans and dogs worldwide. In the Mediterranean basin, Portugal, Central and South America, and in the Middle East, visceral leishmaniosis is caused by Leishmania infantum. In these areas, dogs are believed to be the natural reservoirs of this parasite. In the case of visceral leishmaniosis, the spleen is one of the several hematopoietic and immunocompetent organs involved. ...
Tópico(s): Animal health and immunology
2006 - Wiley | Microscopy Research and Technique
Gabriela Santos‐Gomes, Armanda Rodrigues, Francisco Bruno Teixeira, J. Carreira, Graça Alexandre‐Pires, Sandra Carvalho, David Santos-Mateus, Catarina Martins, Inês Vale-Gato, Cláudia Marques, Ana M. Tomás,
Control of zoonotic visceral leishmaniosis can be achieved using several available drugs. These drugs present high toxicity and require longer treatment regimens which complicate compliance to the treatment. Other control measures directed to the vector or the reservoirs are useful tools to restrain the spreading of this disease but the effects are transitory. A safe, affordable and efficient vaccine conferring long lasting immunity should be the most cost effective way of controlling zoonotic visceral ...
Tópico(s): Toxin Mechanisms and Immunotoxins
2014 - Elsevier BV | Vaccine
David W. Ramilo, Claire Garros, Bruno Mathieu, CHRISTOPHE BENEDET, Xavier Allène, Elisabete Silva, Graça Alexandre‐Pires, Isabel Pereira da Fonseca, Simon Carpenter, Jana Rádrová, Jean‐Claude Delécolle,
A new species, Culicoides paradoxalis Ramilo and Delécolle (Diptera: Ceratopogonidae), is described from specimens collected in France (Corsica and southeast region) and Portugal. This species resembles Culicoides lupicaris Downes and Kettle, and can be distinguished from this species and from Culicoides newsteadi Austen by its wing pattern, in addition to the absence of spines on the tarsomere 4 of female mid leg. In male, the presence of two appendices on the sternite 9 together with the absence ...
Tópico(s): Animal Disease Management and Epidemiology
2013 - Q15088586 | Zootaxa
Produção Nacional
Revisado por pares
Cláudia Marques, Luiz Felipe Domingues Passero, Inês Vale-Gato, Armanda Rodrigues, Olı́via Roos Rodrigues, Catarina Martins, Ivone Correia, Ana M. Tomás, Graça Alexandre‐Pires, Maria Helena Ferronha, Gabriela Santos‐Gomes,
The interaction between polymorphonuclear leukocytes (PMN) or neutrophils and Leishmania became an interesting focus of research, since PMN turn out to be essential cells in transiently hosting the parasites. This study aims to evaluate whether L. infantum, the etiological agent of zoonotic visceral leishmaniasis, influences the in vitro functional activity of murine neutrophils. Phagocytosis, chemotaxis, oxidative burst, degranulation and apoptosis assays were performed. Cytokines, chemokines and ...
Tópico(s): Parasites and Host Interactions
2015 - Elsevier BV | Comparative Immunology Microbiology and Infectious Diseases
Armanda Rodrigues, Mafalda Sofia da Silva Claro, Graça Alexandre‐Pires, David Santos-Mateus, Catarina Martins, Ana Valério-Bolas, Mariana Rafael-Fernandes, Maria A. Pereira, Isabel Pereira da Fonseca, Ana M. Tomás, Gabriela Santos‐Gomes,
In the recent years, the liver has been recognized as an important immune organ with major regulatory functions and immune memory, adding to the well-described vital metabolic functions. There are evidences from experimental infections performed with visceral Leishmania species that immune responses to parasite infection can be organ-specific. The liver is the compartment of acute resolving infection, with minimal tissue damage and resistance to reinfection, whereas the spleen is the compartment ...
Tópico(s): Trypanosoma species research and implications
2016 - Elsevier BV | Immunobiology
Produção Nacional
Revisado por pares
Maria A. Pereira, Ana Valério-Bolas, David Santos-Mateus, Graça Alexandre‐Pires, Marcos Santos, Armanda Rodrigues, Hugo Alexandre Oliveira Rocha, Ana Paula de Azevedo dos Santos, Catarina Martins, Ana M. Tomás, Luiz Felipe Domingues Passero, Isabel Pereira da Fonseca, Gabriela Santos‐Gomes,
Canine leishmaniosis caused by L. infantum is a severe zoonotic disease. Although macrophages are the definitive host cells, neutrophils are the first cells to encounter the parasite soon after its inoculation in the dermis by the phlebotomine vector. To study the interaction of dog neutrophils and L. infantum promastigotes, blood neutrophils were isolated from healthy donors and the infection was established in vitro. In the majority of the dogs, L. infantum was efficiently phagocytized by neutrophils, ...
Tópico(s): Eosinophilic Disorders and Syndromes
2017 - Elsevier BV | Veterinary Parasitology
Maria A. Pereira, Ana Valério-Bolas, Cátia Marques, Graça Alexandre‐Pires, Isabel Pereira da Fonseca, Gabriela Santos‐Gomes,
Immune system recognize and fight back foreign microorganisms and inner modifications that lead to deficient cell and tissue functions. During a dog’s life, the immune system needs to adapt to different physiological conditions, assuring surveillance and protection in a careful and controlled way. Pregnancy alters normal homeostasis, requiring a balance between immunity and tolerance. The embryos and fetus should be protected from infections, while the female dog must tolerate the growing of semi- ...
Tópico(s): Infant Nutrition and Health
2019 - Multidisciplinary Digital Publishing Institute | Veterinary Sciences
Marcos Santos, Graça Alexandre‐Pires, Maria A. Pereira, Cátia Marques, Joana Gomes, Jorge Correia, Ana Duarte, Lídia Gomes, Armanda Rodrigues, Alexandra Basso, Ana Reisinho, José Meireles, David Santos-Mateus, Maria Teresa Villa de Brito, Luís Tavares, Gabriela Santos‐Gomes, Isabel Pereira da Fonseca,
Canine leishmaniosis (CanL) caused by Leishmania infantum is a zoonotic disease of global concern. Antileishmanial drug therapies commonly used to treat sick dogs improve their clinical condition, although when discontinued relapses can occur. Thus, the current study aims to evaluate the effect of CanL treatments in peripheral blood, lymph node, and bone marrow cytokine profile associated with clinical recovery. Two groups of six dogs diagnosed with CanL were treated with miltefosine combined with ...
Tópico(s): Leptospirosis research and findings
2019 - Frontiers Media | Frontiers in Veterinary Science
Produção Nacional
Revisado por pares
Tatiana Dias-Guerreiro, Joana Palma-Marques, Patrícia Mourata-Gonçalves, Graça Alexandre‐Pires, Ana Valério-Bolas, Áurea Martins Gabriel, Telmo Nunes, Wilson Antunes, Isabel Pereira da Fonseca, Marcelo Sousa Silva, Gabriela Santos‐Gomes,
African trypanosomiasis or sleeping sickness is a zoonotic disease caused by Trypanosoma brucei, a protozoan parasite transmitted by Glossina spp. (tsetse fly). Parasite introduction into mammal hosts triggers a succession of events, involving both innate and adaptive immunity. Macrophages (MΦ) have a key role in innate defence since they are antigen-presenting cells and have a microbicidal function essential for trypanosome clearance. Adaptive immune defence is carried out by lymphocytes, especially ...
Tópico(s): Galectins and Cancer Biology
2021 - Multidisciplinary Digital Publishing Institute | Biomedicines
Süleyman Tolga Yavuz, Ozan Koc, Ali Güngör, Faysal Gök, Jessica E. Hawley, Christopher J. O’Brien, Matthew F. Thomas, Malcolm Brodlie, Louise J. Michaelis, Inês Mota, Angéla Gáspár, Susana Piedade, Graça Sampaio, José Geraldo Dias, Miguel Paiva, Mário Morais‐Almeida, Cristina Madureira, Tânia Lopes, Susana Lopes, Filipa Almeida, Alexandra Sequeira, Fernanda Carvalho, José Inácio de Oliveira, Fabienne Gay-Crosier, Ioana-Valentina Nenciu, Andreia Nita, Alexandru Ulmeanu, Dumitru Orăşeanu, Carmen Zăpucioiu, Adrianna Machinena, Olga Domínguez Sánchez, Montserrat Álvaro‐Lozano, Rosa Feijoo, Jaime Lozano Blasco, Mònica Piquer Gibert, M.T. Giner Muñoz, M. Dias da Costa, Ana Martín, Ebru Arık Yılmaz, Özlem Cavkaytar, Betül Büyüktiryaki, Özge Soyer, Cansın Saçkesen, Merryn J. Netting, Adaweyah El-Merhibi, Michael Gold, Patrick Quinn, Irmeli Penttila, Maria Makrides, Stavroula Giavi, Antonella Muraro, Roger Lauener, Annick Mercenier, Eugen Bersuch, Isabella Monia Montagner, Maria Passioti, Nicolò Celegato, Selina Summermatter, Sophie Nutten, Tristan Bourdeau, Yvonne M. Vissers, Nikolaos G. Papadopoulos, Hanneke van der Kleij, Hans Warmenhoven, Ronald van Ree, Raymond Pieters, Dirk Jan E. Opstelten, Hans van Schijndel, Joost J. Smit, Róisín Fitzsimons, Victoria Timms, George Du Toit, Güven Kaya, Mustafa Güleç, Mehmet Saldır, Osman Şener, Nagwa Hassan, Hala Shaaban, Hazem M. El-Hariri, Ahmed Kamel Inas E. Mahfouz, Papp Gabor, G. Bíró, K Csaba, Bo Chawes, Klaus Bønnelykke, Jakob Stokholm, Lene Heickendorff, Susanne Brix, Morten Arendt Rasmussen, Hans Bisgaard, Henrik Wegener Hallas, Lambang Arianto, Maike Pincus, Thomas Keil, Andreas Reich, Ulrich Wahn, Susanne Lau, Linus Grabenhenrich, Sara Fagerstedt, Helena Marell Hesla, Emelie Johansson, Helen Rosenlund, Axel Mie, Annika Scheynius, Johan Alm, Jorge Esparza-Gordillo, Anja Matanovic, Ingo Marenholz, Anja Bauerfeind, Klaus Rohde, Katja Nemat, Min-Ae Lee-Kirsch, Magnus Nordenskjöld, M.C.G. Winge, Renate Krüger, Kirsten Beyer, Birgit Kalb, B. Niggemann, Norbert Hübner, Heather J. Cordell, Maria Bradley, Young‐Ae Lee, Hannah Gough, Dirk Schramm, John Beschorner, Antje Schuster, Carl‐Peter Bauer, Johannes Förster, Fred Zepp, Renate L. Bergmann, Karl‐Christian Bergmann, Filipe Benito Garcia, Natacha Santos, Helena Pité, Athina Papadopoulou, Despina Mermiri, Xatziagorou Elpida, Ioannis Tsanakas, Stavroula Lampidi, Kostas Ν. Priftis, Elaine Fuertes, Iana Markevych, Gayan Bowatte, Olena Gruzieva, Ulrike Gehring, Allan B. Becker, Dietrich Berdel, Michael Bräuer, Chris Carlsten, Barbara Hoffmann, Anita L. Kozyrskyj, Caroline Lodge, Göran Pershagen, Alet H. Wijga, Heinrich Joachim, Zorica Živković, Ivana Djuric-Filipovic, Jasmina Jocić-Stevanovic, Snežana Zivanovic, Styliani Τaka, Dimitra Kokkinou, Aliki Papakonstantinou, Panagiota Stefanopoulou, Anastasia Georgountzou, Paraskevi Maggina, Sofia Stamataki, Vassiliki Papaevanggelou, Evangelos Andreakos, Mònica Piquer Gibert, Adriana Machinena Spera, Matea Deliu, Danielle Belgrave, Angela Simpson, Adnan Ćustović, João Gaspar Marques, Pedro Martins, Joana Belo, Sara Serranho, I Peralta, Nuno Neuparth, Paula Pinto, Marta Vázquez‐Ortiz, Mariona Pascal, Ana María Plaza, Manel Juan, Lorella Paparo, Rita Nocerino, Rosita Aitoro, I. Langella, Antonio Amoroso, Alessia Amoroso, Carmen Di Scala, Roberto Berni Canani, Santanu Maity, Giuseppina Rotiroti, Minal Gandhi, Karin Jönsson, Annika Ljung, Bill Hesselmar, Ingegerd Adlerbert, Hilde K. Brekke, Susanne Johansen, Agnes E. Wold, Ann‐Sofie Sandberg, Björn Nordlund, Cecilia Lundholm, Villhelmina Ullemar, Marianne van Hage, Anne K. Örtqvist, Catarina Almqvist, Anna Selby, Kate Grimshaw, Michael Clausen, Rūta Dubakienė, Alessandro Fiocchi, Marek L. Kowalski, Nikolaos G. Papadopoulos, Marta Reche, Sigurveig Sigurdardottir, Aline Sprikkleman, Paraskevi Xepapadaki, E. N. Clare Mills, Graham Roberts, Herberto José Chong‐Neto, Gustavo Falbo Wandalsen, Ana Carolina Dela Bianca, Carolina Sánchez Aranda, Nelson Augusto Rosário Filho, Dirceu Solé, Javier Mallol, Luis García‐Marcos, Ivana Banić, Matija Rijavec, Davor Plavec, Peter Korošec, Mirjana Turkalj, Alen Božičević, Maria De Mieri, Matthias Hamburger, Simone Holley, Ruth Morris, Frances Mitchell, Rebecca Knibb, Sue Latter, Christina Liossi, Mostafa M. Hassan, Malin Barman, Anna Sandin, Daniela Posa, Serena K. Perna, Ute Hoffmann, Kuan‐Wei Chen, Yvonne Resch, Susanne Vrtala, Rudolf Valenta, Paolo Maria Matricardi, Olympia Tsilochristou, Alexander Rohrbach, Antonio Cappella, Stephanie Hofmaier, Laura Hatzler, Raffaele D’Amelio, Sophia Björkander, Maria A. Johansson, Gintare Lasaviciute, Eva Sverremark‐Ekström, Franz Rüschendorf, David P. Strachan, Ben D. Spycher, Hansjörg Baurecht, Patricia Margaritte‐Jeannin, Annika Sääf, Marjan Kerkhof, Markus Ege, Svetlana Baltic, Melanie C. Matheson, Jin Li, Sven Michel, Wei Ang, Wendy L. McArdle, Andreas Arnold, Georg Homuth, Florence Démenais, Emmanuelle Bouzigon, Cilla Söderhäll, Johan C. de Jongste, Dirkje S. Postma, Charlotte Braun‐Fahrländer, Elisabeth Horak, Л. М. Огородова, В. П. Пузырев, E. Yu. Bragina, Thomas J. Hudson, Charles Morin, David L. Duffy, Guy B. Marks,
... Gaspar, Graça Sampaio, Cristina Arêde, Luís Miguel Borrego, Graça Pires, Cristina Santa-Marta, Mário Morais-Almeida POSTER DISCUSSION ...
Springer Science+Business Media
Produção Nacional
Revisado por pares
Maria A. Pereira, Graça Alexandre‐Pires, Murilo Moraes da Câmara, Marcos Santos, Catarina Martins, Armanda Rodrigues, Jéssica Adriana, Luiz Felipe Domingues Passero, Isabel Pereira da Fonseca, Gabriela Santos‐Gomes,
Leishmania infantum is the aetiological agent of human visceral leishmaniasis and canine leishmaniasis, both systemic and potentially fatal diseases. Polymorphonuclear neutrophils (PMN) are the first cells to phagocyte this parasite at the inoculation site, but macrophages (MØ) are the definitive host cells, ensuring parasite replication. The interaction between dog MØ, PMN and L infantum promastigotes was in vitro investigated. It was observed that promastigotes establish contact with blood monocyte- ...
Tópico(s): Eosinophilic Disorders and Syndromes
2019 - Wiley | Parasite Immunology
Maria Rosa Rebordão, Graça Alexandre‐Pires, Montserrat Lopez Carreira, L. Adriano, Carla Carneiro, Teresa Nunes, L. Mateus, Graça Ferreira‐Dias,
Neutrophils are capable of releasing their DNA in response to infectious agents to form neutrophil extracellular traps (NETs) to destroy pathogens. Even though pyometra in queens and bitches is a common disease, its pathogenesis is not fully understood. The aim of this study was to assess the presence of NETs in the endometrium of queens and bitches suffering from pyometra. Pyometra and normal uteri were obtained after ovariohysterectomy from adult queens and bitches in diestrus. Uterine contents ...
Tópico(s): Immune Response and Inflammation
2017 - Elsevier BV | Veterinary Immunology and Immunopathology
Marı́a del Mar Yllera, Graça Alexandre‐Pires, Manuel Cifuentes,
Abstract Mammalian blastocyst produces membranes that gradually attach in the endometrial epithelium and establish a close relationship between fetal and maternal circulatory systems for physiologic exchange. This fact results in the formation of a combined organ, the placenta. Placentation includes extensive neovascularization in maternal and embryonic placental tissues. Recent studies, particularly of genetically altered mice, have greatly improved our understanding of the molecular basis of the ...
Tópico(s): Congenital heart defects research
2002 - Wiley | Microscopy Research and Technique
P. Ramos, Raquel L. Carvalho, Fernanda Rosa, Graça Alexandre‐Pires, Fernanda Seixas, Alexandra Esteves, David G. Huffman,
Some pouting caught off the Atlantic coast of Portugal are discarded as unmarketable due to a dark discolouration of the skin and muscle. This study investigates the cause of this condition, describes the new parasite species responsible, and highlights the importance of educating those in charge of premarket inspection of food fish in order to reduce likelihood that consumers will eat infected fish. Macroscopically, infected fish showed considerable heterogeneity in darkening of the skin and hypaxial ...
Tópico(s): Helminth infection and control
2019 - Elsevier BV | International Journal for Parasitology Parasites and Wildlife
Suraya Diaz, Isabel Pereira da Fonseca, Armanda Rodrigues, Catarina Martins, Clara Cartaxeiro, Maria Jesus Silva, Teresa Villa de Brito, Graça Alexandre‐Pires, Gabriela Santos‐Gomes,
Canine leishmaniosis, caused by Leishmania infantum, is a systemic disease with variable clinical signs and a progressive evolution. This disease is characterized by impaired T cell-mediated immune response, which has been associated with disease chronicity and high mortality. Protective immunity against leishmaniosis is thought to be mediated by T cell and cytokine production. The T cell activation requires a primary signal delivered by the major histocompatibility complex (MHC) molecules present ...
Tópico(s): Animal health and immunology
2012 - Elsevier BV | Veterinary Parasitology
Jordi Miró, Miguel A. Gutiérrez-Reinoso, Joana Aguiar da Silva, Carina Fernandes, Maria Rosa Rebordão, Graça Alexandre‐Pires, Jaime Catalán, Graça Ferreira‐Dias,
Collagen fibers and inflammatory cells are the basis for jenny endometrium Kenney and Doig's classification developed for the mare. The infiltration of a large number of eosinophils in the jenny endometrium is intriguing. Eosinophil and fibroblast produced IL33, which has been related to fibrosis development and chronicity. This work on the endometrium consisted of (i) quantification of collagen type I (COL1A2), type III (COL3A1), and IL33 transcripts; (ii) histological localization and quantification ...
Tópico(s): Reproductive Physiology in Livestock
2020 - Frontiers Media | Frontiers in Veterinary Science
Produção Nacional
Revisado por pares
Armanda Rodrigues, David Santos-Mateus, Graça Alexandre‐Pires, Ana Valério-Bolas, Mariana Rafael-Fernandes, Maria A. Pereira, Dário Ligeiro, Jorge de Jesús, Raquel Azevedo, Sara Lopes‐Ventura, Marcos Santos, Ana M. Tomás, Isabel Pereira da Fonseca, Gabriela Santos‐Gomes,
Kupffer cells (KC) are the liver macrophage population that resides in the hepatic sinusoids and efficiently phagocyte pathogens by establishing an intimate contact with circulating blood. KC constitute the liver host cells in Leishmania infection, nevertheless little is described about their role, apart from their notable contribution in granulomatous inflammation. The present study aims to investigate how canine KC sense and react to the presence of Leishmania infantum promastigotes and amastigotes ...
Tópico(s): Parasites and Host Interactions
2017 - Elsevier BV | Comparative Immunology Microbiology and Infectious Diseases
Produção Nacional
Revisado por pares
Ana Valério-Bolas, Maria A. Pereira, Graça Alexandre‐Pires, David Santos-Mateus, Armanda Rodrigues, Mariana Rafael-Fernandes, Áurea Martins Gabriel, Luiz Felipe Domingues Passero, Gabriela Santos‐Gomes,
Neutrophils are short-lived phagocytic cells equipped with several receptors for pathogen recognition and phagocytosis and have intracellular and extracellular effector mechanisms that can inactivate pathogens. Leishmaniases are diseases caused by different species of Leishmania that mainly afflicts poorer populations of tropical and subtropical regions and immunocompromised individuals. Thus, the present study aims to investigate the effector response of murine neutrophils to species of Leishmania ...
Tópico(s): Eosinophilic Disorders and Syndromes
2018 - Elsevier BV | Cellular Immunology
Juliana Inês Weber, Armanda Rodrigues, Ana Valério-Bolas, Telmo Nunes, Manuela Carvalheiro, Wilson Antunes, Graça Alexandre‐Pires, Isabel Pereira da Fonseca, Gabriela Santos‐Gomes,
Leishmaniasis is a parasitic disease caused by different species of Leishmania and transmitted through the bite of sand flies vector. Macrophages (MΦ), the target cells of Leishmania parasites, are phagocytes that play a crucial role in the innate immune microbial defense and are antigen-presenting cells driving the activation of the acquired immune response. Exploring parasite–host communication may be key in restraining parasite dissemination in the host. Extracellular vesicles (EVs) constitute ...
Tópico(s): Research on Leishmaniasis Studies
2023 - Multidisciplinary Digital Publishing Institute | Cells
Produção Nacional
Revisado por pares
Graça Alexandre‐Pires, Diogo Pais, J. A. Esperança Pina,
Numerous studies have been made regarding circulation via the red pulp of the spleen, and intense controversy surrounds the question as to whether or not endothelial continuity exists between arterial and venous vessels. Aware of this intense controversy, and in order to perform investigation over the spleen of dogs infected with a parasitic disease (future reports shall be done), the authors studied the vascularization of the normal dog spleen in order to define its normal pattern and evaluate the ...
Tópico(s): Comparative Animal Anatomy Studies
2003 - Wiley | Anatomia Histologia Embryologia
Armanda Rodrigues, Graça Alexandre‐Pires, Ana Valério-Bolas, David Santos-Mateus, Mariana Rafael-Fernandes, Maria A. Pereira, Dário Ligeiro, Telmo Nunes, Raquel Azevedo, Sara Lopes‐Ventura, Marcos Santos, Ana M. Tomás, Isabel Pereira da Fonseca, Gabriela Santos‐Gomes,
Abstract Hepatocytes constitute the majority of hepatic cells, and play a key role in controlling systemic innate immunity, via pattern-recognition receptors (PRRs) and by synthesizing complement and acute phase proteins. Leishmania infantum , a protozoan parasite that causes human and canine leishmaniasis, infects liver by establishing inside the Kupffer cells. The current study proposes the elucidation of the immune response generated by dog hepatocytes when exposed to L. infantum . Additionally, the ...
Tópico(s): Hepatitis Viruses Studies and Epidemiology
2018 - Cambridge University Press | Parasitology
João Durães-Oliveira, Joana Palma-Marques, Cláudia Jassica Gonçalves Moreno, Armanda Rodrigues, Marta Monteiro, Graça Alexandre‐Pires, Isabel Pereira da Fonseca, Gabriela Santos‐Gomes,
Chagas disease (CD) is a vector-borne Neglected Zoonotic Disease (NZD) caused by a flagellate protozoan, Trypanosoma cruzi, that affects various mammalian species across America, including humans and domestic animals. However, due to an increase in population movements and new routes of transmission, T. cruzi infection is presently considered a worldwide health concern, no longer restricted to endemic countries. Dogs play a major role in the domestic cycle by acting very efficiently as reservoirs ...
Tópico(s): Insect symbiosis and bacterial influences
2024 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Graça Martinho, Ana Pires, Luanha Saraiva, Rita A. Ribeiro,
This paper describes a direct analysis study carried out in a recycling unit for waste electrical and electronic equipment (WEEE) in Portugal to characterize the plastic constituents of WEEE. Approximately 3400 items, including cooling appliances, small WEEE, printers, copying equipment, central processing units, cathode ray tube (CRT) monitors and CRT televisions were characterized, with the analysis finding around 6000 kg of plastics with several polymer types. The most common polymers are polystyrene, ...
Tópico(s): Municipal Solid Waste Management
2012 - Elsevier BV | Waste Management