... 106227Antonio Contreras, Juan J. Muñoz-Perez, Francisco Contreras, Gregorio Gomez-Pina, Veronica Ruiz-Ortiz, Gabriel Chamorro, Pablo Cabrera ...
Tópico(s): Aeolian processes and effects
1902 - University of Chicago Press | The Journal of Geology
Christopher J. Occhiuto, Ananth Kumar Kammala, Canchai Yang, Rithvik Nellutla, Marco Garcia, Gregorio Gomez, Hariharan Subramanian,
Mast cells are inflammatory immune cells that play an essential role in mediating allergic reactions in humans. It is well known that mast cell activation is critically regulated by intracellular calcium ion (Ca2+) concentrations. MAS-related G-protein coupled receptor-X2 (MRGPRX2) is a G-protein coupled receptor (GPCR) expressed on mast cells that is activated by various ligands, including several FDA approved drugs; consequently, this receptor has been implicated in causing pseudo-allergic reactions ...
Tópico(s): Allergic Rhinitis and Sensitization
2020 - Frontiers Media | Frontiers in Immunology
Ahmed Dawood Mohammed, M.A. Wadud Khan, Ioulia Chatzistamou, Douja Chamseddine, Katie N. Kang, Mason Perry, Reilly T. Enos, Angela Murphy, Gregorio Gomez, Ahmed Al-Adhami, Carole A. Oskeritzian, Amy Jolly, Yan Chang, Shuqian He, Zui Pan, Jason L. Kubinak,
Primary immunodeficiencies are heritable disorders of immune function. CD19 is a B cell co-receptor important for B cell development, and CD19 deficiency is a known genetic risk factor for a rare form of primary immunodeficiency known as 'common variable immunodeficiency' (CVID); an antibody deficiency resulting in low levels of serum IgG and IgA. Enteropathies are commonly observed in CVID patients but the underlying reason for this is undefined. Here, we utilize CD19-/- mice as a model of CVID to ...
Tópico(s): Cystic Fibrosis Research Advances
2019 - Frontiers Media | Frontiers in Immunology
Cody McHale, Zahraa Mohammed, Gregorio Gomez,
Mast cells are classically recognized as cells that cause IgE-mediated allergic reactions. However, their ability to store and secrete vascular endothelial growth factor (VEGF) suggests a role in vascular development and tumorigenesis. The current study sought to determine if other angiogenesis-related factors, in addition to VEGF, were also secreted by human tissue-derived mast cells. Using proteome array analysis and ELISA, we found that human skin-derived mast cells spontaneously secrete CXCL16, ...
Tópico(s): Polyamine Metabolism and Applications
2019 - Frontiers Media | Frontiers in Immunology
Allergies and asthma are a major cause of chronic disease whose prevalence has been on the rise. Allergic disease including seasonal rhinitis, atopic dermatitis, urticaria, anaphylaxis, and asthma, are associated with activation of tissue resident mast cells and circulating basophils. Although these cells can be activated in different ways, allergic reactions are normally associated with the crosslinking of the high affinity Fc receptor for Immunoglobulin E, FcεRI, with multivalent antigen. Inflammatory ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2019 - Frontiers Media | Frontiers in Immunology
Cody McHale, Zahraa Mohammed, Juline Deppen, Gregorio Gomez,
Interleukin-6 is a gp130 utilizing cytokine that is consistently associated with allergic diseases like asthma and urticaria in humans where mast cells are known to play a critical role. However, the role of IL-6 in allergic disease in not known. IL-6 was reported to enhance degranulation of in vitro-derived mast cells, but the effect of IL-6 on mediator release from human in situ-matured tissue-isolated mast cells had not been reported. Human mature mast cells were isolated and purified from normal ...
Tópico(s): PI3K/AKT/mTOR signaling in cancer
2018 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - General Subjects
Charlotte Vanmarsenille, Jelle Elseviers, Charlotte Yvanoff, Gholamreza Hassanzadeh‐Ghassabeh, Gregorio Gómez-Jarabo García, Edo Martens, Ann Depicker, An Martel, Freddy Haesebrouck, Frank Pasmans, Jean-Pierre Hernálsteens, Henri De Greve,
Campylobacteriosis is a widespread infectious disease, leading to a major health and economic burden. Chickens are considered as the most common infection source for humans. Campylobacter mainly multiplies in the mucus layer of their caeca. No effective control measures are currently available, but passive immunisation of chickens with pathogen-specific maternal IgY antibodies, present in egg yolk of immunised chickens, reduces Campylobacter colonisation. To explore this strategy further, anti-Campylobacter ...
Tópico(s): Escherichia coli research studies
2018 - Public Library of Science | PLoS ONE
Milena Vainieri, Federico Vola, Gregorio Gomez Soriano, Sabina Nuti,
The definition of "the right targets" and the way the evaluation of results is performed affect the willingness to commit to new challenges, which is a factor that influences the relationship between goal setting and performance results. Indeed, some authors claim that the choice of an inappropriate goal-setting procedure is a major cause of failure of management control systems. Goal setting theorists found that assigning a specific and challenging goal leads to higher performance than (a) an easy ...
Tópico(s): Healthcare Quality and Management
2016 - Elsevier BV | Health Policy
Andrea Troupin, Devon L. Shirley, Berlin Londoño-Rentería, Alan M. Watson, Cody McHale, Alex R. Hall, Adam Hartstone‐Rose, William B. Klimstra, Gregorio Gomez, Tonya M. Colpitts,
Dengue virus (DENV) is a mosquito-borne flavivirus that causes serious global human disease and mortality. Skin immune cells are an important component of initial DENV infection and systemic spread. Here, we show that mast cells are a target of DENV in human skin and that DENV infection of skin mast cells induces degranulation and alters cytokine and growth factor expression profiles. Importantly, to our knowledge, we also demonstrate for the first time that DENV localizes within secretory granules ...
Tópico(s): Insect symbiosis and bacterial influences
2016 - American Association of Immunologists | The Journal of Immunology
Devon L. Shirley, Cody McHale, Gregorio Gomez,
Resveratrol, a natural polyphenol found in the skin of red grapes, is reported to have anti-inflammatory properties including protective effects against aging. Consequently, Resveratrol is a common nutritional supplement and additive in non-prescription lotions and creams marketed as anti-aging products. Studies in mice and with mouse bone marrow-derived mast cells (BMMCs) have indicated anti-allergic effects of Resveratrol. However, the effects of Resveratrol on human primary mast cells have not ...
Tópico(s): Sirtuins and Resveratrol in Medicine
2016 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - General Subjects
R. Hernández, E. Rosendo, R. Romano‐Trujillo, A.I. Oliva, Gregorio Gómez-Jarabo García, F.G. Nieto-Caballero, T. Díaz, Crisóforo Morales, H. Juárez, M. Pacio, R. Galeazzi,
Abstract Structural, morphological and compositional characterizations of zinc selenide nanoparticles (NPs-ZnSe) are discussed in this work. NPs-ZnSe were obtained by colloidal synthesis in aqueous solution at room temperature, atmospheric pressure and without inert atmosphere. The synthesis was carried out using zinc chloride (ZnCl 2 ) and elemental selenium as precursors. A mix of Na 5 P 3 O 10 and NaOH called Extran was used as surfactant. Molar concentration and pH of the aqueous solution were varied ...
Tópico(s): Copper-based nanomaterials and applications
2015 - Elsevier BV | Materials Letters
Juan José Muñoz Pérez, A.B.M. Khan-Mozahedy, M. G. Neves, B. Tejedor, Gregorio Gómez-Pina, José María Del Campo, V. Negro,
Three submerged coastal structures on the sandy seabed at Santa Maria del Mar (SMM) Beach in Southwest Spain were monitored during the six months after installation, starting in November of 2005. The monitoring evaluated the self-burial phenomenon of the 40 precast modular concrete elements of the three structures. Assessment included scouring around the structures, their vertical movement, the sinking velocity of the modules and the resulting beach profiles. Pressure sensors and precision topographies ...
Tópico(s): Smart Materials for Construction
2015 - Elsevier BV | Coastal Engineering
Gregorio Gomez, Vincent C. Nardone, Sahar Lotfi‐Emran, Wei Zhao, Lawrence B. Schwartz,
Adenosine (ADO) can enhance and inhibit mast cell degranulation. Potentiation of degranulation occurs at relatively low concentrations of ADO (10−6–10−5 M) through triggering of A3AR, whereas, inhibition occurs at higher concentrations of ADO reportedly through triggering of A2aAR. However, the discrepancy in the concentration of ADO that inhibits degranulation and that required to trigger ADORs suggests a different mechanism. The purpose of this study is to determine the mechanism by which ADO inhibits ...
Tópico(s): Receptor Mechanisms and Signaling
2013 - Springer Science+Business Media | Journal of Clinical Immunology
R. Romano‐Trujillo, E. Rosendo, Mauricio Ortega, A. Morales–Sánchez, José Gracia, T. Díaz, F.G. Nieto-Caballero, Gregorio Gómez-Jarabo García, José Alberto Luna López, M. Pacio,
Lead selenide nanoparticles (PbSe NPs) have been obtained through an easy and low cost route using colloidal synthesis in aqueous solution. The synthesis was carried out at room temperature using Extran (Na₅P₃O₁₀, NaOH and H₂O) as surfactant. Hydrochloric acid (HCl) was used to eliminate the generated by-products. The size of PbSe NPs was varied by changing the Pb:Se molar concentration. The PbSe NPs were characterized by powder x-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive ...
Tópico(s): Advanced Thermoelectric Materials and Devices
2012 - IOP Publishing | Nanotechnology
Quang Le, Gregorio Gomez, Wei Zhao, Jiang Hu, Han-Zhang Xia, Yoshihiro Fukuoka, Nobuhiko Katunuma, Lawrence B. Schwartz,
Abstract Human β-tryptase is stored in secretory granules of human mast cells as a heparin-stabilized tetramer. β-Protryptase in solution can be directly processed to the mature enzyme by cathepsin (CTS) L and CTSB, and sequentially processed by autocatalysis at R−3, followed by CTSC proteolysis. However, it is uncertain which CTS is involved in protryptase processing inside human mast cells, because murine bone marrow-derived mast cells from CTSC-deficient mice convert protryptase (pro–mouse mast ...
Tópico(s): Coagulation, Bradykinin, Polyphosphates, and Angioedema
2011 - American Association of Immunologists | The Journal of Immunology
Jorge Román‐Sierra, Marina Navarro‐Pons, Juan José Muñoz Pérez, Gregorio Gómez-Pina,
Beach-nourishment requirements on the southwestern Spanish coast have led to a significant increase in offshore dredging. Following a new research line, assessment of changes recorded in physicochemical and biological parameters due to dredging and dumping operations was performed at the Cape of Trafalgar and Palmar Beach during June and July 2008. Turbidity, salinity, pH, dissolved oxygen, temperature, and suspended-sediment data were collected at 10 stations. At the end of the study, a three-campaign ...
Tópico(s): Marine and fisheries research
2011 - American Society of Civil Engineers | Journal of Waterway Port Coastal and Ocean Engineering
Wei Zhao, Gregorio Gomez, Matthew Macey, Christopher L. Kepley, Lawrence B. Schwartz,
Desensitization is a clinical procedure whereby incremental doses of a drug are administered over several hours to a sensitive patient until a therapeutic dose and clinical tolerance are achieved. Clinical tolerance may occur in part by attenuating the mast cell response. In the present study, primary human skin mast cells were used to establish and characterize an in vitro model of desensitization. Mast cells in culture were armed with allergen-specific (4-hydroxy-3-nitrophenylacety and Der p2) ...
Tópico(s): Asthma and respiratory diseases
2011 - Springer Science+Business Media | Journal of Clinical Immunology
Gregorio Gomez, Wei Zhao, Lawrence B. Schwartz,
Inhaled and intravenously administered adenosine induces mast cell-mediated (histamine-dependent) bronchospasm in asthmatics without causing urticaria. A differential response to adenosine by human lung and skin mast cells is shown: low concentrations potentiate FcεRI-induced degranulation of human lung mast cells but not that of skin mast cells. Human lung mast cells were found to express ∼3-fold more A3AR messenger RNA (mRNA) than skin mast cells, suggesting the involvement of the Gi-linked A3AR. ...
Tópico(s): Asthma and respiratory diseases
2011 - Springer Science+Business Media | Journal of Clinical Immunology
Marina Navarro‐Pons, Juan José Muñoz Pérez, Jorge Román‐Sierra, Haim Tsoar, Inmaculada Rodríguez, Gregorio Gómez-Pina,
Dune activity or stability has usually been studied over long time periods; however, this may not reflect changes that occur in the short term, especially for highly active dunefields. Extreme wind conditions that are generated near the Strait of Gibraltar (SW Spain) have given rise to the transgressive Valdevaqueros dunefield. The current work focuses on analyzing the sand drift potential and the evolution of the dune profile in the medium term (months), the short term (days) and the very short term ( ...
Tópico(s): Soil erosion and sediment transport
2011 - Elsevier BV | Geomorphology
Matthew Macey, Jamie Sturgill, Johanna K. Morales, Yves T. Falanga, Joshua J. Morales, Sarah K. Norton, Nitin Yerram, Hoon Shim, Josephine Fernando, Alasdair M. Gifillan, Gregorio Gomez, Lawrence B. Schwartz, Carole A. Oskeritzian, Sarah Spiegel, Daniel H. Conrad, John Ryan,
Mast cell responses can be altered by cytokines, including those secreted by Th2 and regulatory T cells (Treg). Given the important role of mast cells in Th2-mediated inflammation and recent demonstrations of Treg-mast cell interactions, we examined the ability of IL-4 and TGF-beta1 to regulate mast cell homeostasis. Using in vitro and in vivo studies of mouse and human mast cells, we demonstrate that IL-4 suppresses TGF-beta1 receptor expression and signaling, and vice versa. In vitro studies demonstrated ...
Tópico(s): Allergic Rhinitis and Sensitization
2010 - American Association of Immunologists | The Journal of Immunology
Nicolas Charles, Wendy T. Watford, Haydeé L. Ramos, Lars Hellman, Hans C. Oettgen, Gregorio Gomez, John J. Ryan, John J. O’Shea, Juan Rivera,
T helper 1 (Th1)-Th2 cell balance is key to host defense and its dysregulation has pathophysiological consequences. Basophils are important in Th2 cell differentiation. However, the factors controlling the onset and extent of basophil-mediated Th2 cell differentiation are unknown. Here, we demonstrate that Lyn kinase dampened basophil expression of the transcription factor GATA-3 and the initiation and extent of Th2 cell differentiation. Lyn-deficient mice had a marked basophilia, a constitutive ...
Tópico(s): Immune Cell Function and Interaction
2009 - Cell Press | Immunity
Juan José Muñoz Pérez, Marina Navarro‐Pons, Jorge Román‐Sierra, B. Tejedor, Inmaculada Rodríguez, Gregorio Gómez-Pina,
The advance of the Valdevaqueros transgressive dune has resulted in gradual invasion of the adjacent road and ecosystem. Despite restoration and control activities performed within the last decades, strong winds have favoured dune destabilisation. Topographic surveys of five profiles were carried out in the research area from 1995 to 2006 to study the dune evolution. It was found that the average migration rate is 17.5 m/year, with a maximum of 38.0 m/year in the most active profile. The Empirical ...
Tópico(s): Soil erosion and sediment transport
2009 - Elsevier BV | Geomorphology
José Carlos Bouso, Rick Doblin, Magı́ Farré, Miguel Ángel Alcázar‐Córcoles, Gregorio Gómez-Jarabo,
Abstract The purpose of this study was to investigate the safety of different doses of MDMA-assisted psychotherapy administered in a psychotherapeutic setting to women with chronic PTSD secondary to a sexual assault, and also to obtain preliminary data regarding efficacy. Although this study was originally planned to include 29 subjects, political pressures led to the closing of the study before it could be finished, at which time only six subjects had been treated. Preliminary results from those ...
Tópico(s): Forensic Toxicology and Drug Analysis
2008 - Taylor & Francis | Journal of Psychoactive Drugs
Wei Zhao, Gregorio Gomez, Shaohua Yu, John J. Ryan, Lawrence B. Schwartz,
Abstract TGF-β has pleiotropic effects on many cell types at different stages of their development, including mast cells. The present study examines the effects of TGF-β on human skin mast cells of the MCTC type. The expression of TGF-β receptors (TGF-R) was verified at the mRNA and protein levels for TGF-RI and TGF-RII, and at the mRNA level for accessory molecules β-glycan and endoglin. TGF-β did not affect mast cell viability after 1 wk at concentrations ≤10 ng/ml, but at 50 ng/ml caused significant ...
Tópico(s): TGF-β signaling in diseases
2008 - American Association of Immunologists | The Journal of Immunology
Gregorio Gomez, Sherryline Jogie‐Brahim, Mika Shima, Lawrence B. Schwartz,
Abstract The dramatic effects of the anti-IgE mAb omalizumab to lower free IgE levels and FcεRI levels on basophils contrast with more modest clinical effects. Accordingly, whether IgE modulates FcεRI levels and FcεRI-dependent mediator release in vitro on human skin mast cells (MCTC type) that had matured in vivo is of interest. IgE reversibly enhanced FcεRI levels on MCTC cells in a dose- and time-dependent manner (up-regulation t1/2 of 4–5 days with 1–3 μg/ml IgE), without affecting cell proliferation. ...
Tópico(s): Mast cells and histamine
2007 - American Association of Immunologists | The Journal of Immunology
John J. Ryan, Mohit Kashyap, Daniel P. Bailey, Sarah Kennedy, Kelly Speiran, Jennifer Brenzovich, Brian Barnstein, Carole A. Oskeritzian, Gregorio Gomez,
Mast cells are well known for their role in allergic disease and have recently been implicated in inflammatory disorders, including autoimmune arthritis, multiple sclerosis, and atherosclerosis. Although aberrant mast cell activation is the focus of many studies, much less is known about normal mast cell homeostasis. Because loss of the normal constraints on mast cell activation, proliferation, and survival may be central to disease etiology, understanding these issues warrants attention. This review ...
Tópico(s): Asthma and respiratory diseases
2007 - Begell House | Critical Reviews in Immunology
Débora González, Jordi Riba, José Carlos Bouso, Gregorio Gómez-Jarabo, Manel J. Barbanoj,
Salvia divinorum is a member of the Lamiaceae family and contains the psychotropic diterpene and kappa-opioid receptor agonist salvinorin-A. Originally a shamanic inebriant used by the Mexican Mazatec Indians, the plant and its preparations are becoming increasingly popular among non-traditional users. Demographic data and information on pattern of use and subjective effects were obtained by means of self-report questionnaires from a sample of 32 recreational users of salvia and other psychedelics. ...
Tópico(s): Alkaloids: synthesis and pharmacology
2006 - Elsevier BV | Drug and Alcohol Dependence
Gregorio Gomez, Carlos Ramírez, Juan Rivera, Manish Patel, Farnaz Norozian, Harry V. Wright, Mohit Kashyap, Brian Barnstein, Krista Fischer‐Stenger, Lawrence B. Schwartz, Christopher L. Kepley, John Ryan,
Abstract Mast cell activation through the high affinity IgE receptor (FcεRI) is a critical component of atopic inflammation. The cytokine TGF-β1 has been shown to inhibit IgE-dependent mast cell activation, possibly serving to dampen mast cell-mediated inflammatory responses. We present proof that TGF-β1 inhibits mast cell FcεRI expression through a reversible pathway that diminishes protein, but not mRNA, expression of the FcεRI subunit proteins α, β, and γ. The stability of the expressed proteins ...
Tópico(s): Cell Adhesion Molecules Research
2005 - American Association of Immunologists | The Journal of Immunology
Gregorio Gomez, Claudia González‐Espinosa, Sandra Odom, Gabriela Pérez Báez, María Eugenia Cid, John J. Ryan, Juan Rivera,
Abstract Fyn kinase is a key contributor in coupling FcεRI to mast cell degranulation. A limited macroarray analysis of FcεRI-induced gene expression suggested potential defects in lipid metabolism, eicosanoid and glutathione metabolism, and cytokine production. Biochemical analysis of these responses revealed that Fyn-deficient mast cells failed to secrete the inflammatory eicosanoid products leukotrienes B4 and C4, the cytokines IL-6 and TNF, and chemokines CCL2 (MCP-1) and CCL4 (MIP-1β). FcεRI- ...
Tópico(s): Autoimmune Bullous Skin Diseases
2005 - American Association of Immunologists | The Journal of Immunology
Yasuko Furumoto, Gregorio Gomez, Claudia González‐Espinosa, Martina Kovářová, Sandra Odom, John Ryan, Juan Rivera,
Src family protein tyrosine kinases (SrcPTK) play a central role in immunoglobulin E (IgE)-mediated activation of mast cells. Functional coupling of the high-affinity IgE receptor (FcεRI) is initiated by the SrcPTK family member, Lyn, through an antigen aggregation-dependent transphosphorylation. Because Lyn is the ‘initiating’ kinase, an essential role in mast cell effector function was conferred. Recent studies challenge this view. Evidence demonstrating that Lyn kinase is dispensable for mast cell ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2005 - Wiley | Novartis Foundation symposium