Ana Marta Rodrigues, Rita Dias Guardão Franca, Madalena Dionı́sio, Chantal Sevrin, Christian Grandfils, Maria A.M. Reis, Nídia D. Lourenço,
Polyhydroxyalkanoates (PHA) are biopolymers with potential to replace conventional oil-based plastics. However, PHA high production costs limit their scope of commercial applications. Downstream processing is currently the major cost factor for PHA production but one of the least investigated aspects of the PHA production chain. In this study, the extraction of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced at pilot scale by a mixed microbial culture was performed using sodium hydroxide (NaOH) ...
Tópico(s): Recycling and Waste Management Techniques
2022 - Multidisciplinary Digital Publishing Institute | Polymers
Produção Nacional
Revisado por pares
Gustavo Gomes Thomé, Talissa Bianchini, Rafael Nazário Bringhenti, Pedro Guilherme Schaefer, Elvino José Guardão Barros, Francisco Veríssimo Veronese,
The prevalence and distribution of glomerular diseases differ among countries, and the indication to perform a kidney biopsy varies among centres. In this study, we assessed the prevalence of primary and secondary glomerulopathies based on histological diagnoses, and the correlation between glomerulopathies and demographic and clinical data was evaluated.In this study, 1051 kidney biopsies were retrospectively reviewed between 2000 and 2018. Patient demographic, clinical and laboratory data were ...
Tópico(s): Chronic Kidney Disease and Diabetes
2021 - BioMed Central | BMC Nephrology
Rita Dias Guardão Franca, H.M. Pinheiro, Nídia D. Lourenço,
Tópico(s): Nanomaterials for catalytic reactions
2020 - Springer Science+Business Media | Reviews in Environmental Science and Bio/Technology
Rita Dias Guardão Franca, A. Vieira, Gilda Carvalho, Adrian Oehmen, H.M. Pinheiro, Maria Teresa Barreto Crespo, Nídia D. Lourenço,
The biodegradation of dyes remains one of the biggest challenges of textile wastewater. Azo dyes are one of the most commonly employed dye classes, and biological treatment processes tend to generate recalcitrant aromatic amines, which are more toxic than the parent dye molecule. This study aimed to isolate bacterial strains with the capacity to degrade both the azo dye and the resulting aromatic amines towards the development of a simple and reliable treatment approach for dye-laden wastewaters. ...
Tópico(s): Chromium effects and bioremediation
2019 - Elsevier BV | Ecotoxicology and Environmental Safety
Rita Dias Guardão Franca, M. Conceição Oliveira, H.M. Pinheiro, Nídia D. Lourenço,
This study focused on the biodegradation of an azo dye (Acid Red 14, AR14) in two anaerobic–aerobic sequencing batch reactors (SBRs) treating synthetic textile wastewater, operated with aerobic granular sludge under different hydrodynamic regimens. The aim was to investigate the fate of the anaerobic AR14 breakdown products (aromatic amines) during the SBRs' aerobic reaction phase. Specifically, liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) was used ...
Tópico(s): Advanced oxidation water treatment
2019 - American Chemical Society | ACS Sustainable Chemistry & Engineering
Nelson Andrade, Cláudia Marques, Sara Andrade, Cláudia Silva, Ilda Rodrigues, Luísa Guardão, João Tiago Guimarães, Elisa Keating, Conceição Calhau, Fátima Martel,
Intake of fructose-containing sugars is epidemiological and experimentally linked to metabolic syndrome (MS). We recently verified that the dietary polyphenol chrysin was able to abolish some of the metabolic changes induced by fructose-feeding in the rat. Because the role of the intestine upon fructose-induced MS is poorly understood, we decided to investigate the influence of fructose, in vivo, on the intestinal environment and the ability of chrysin to interfere with the putative observed changes. ...
Tópico(s): Diet and metabolism studies
2019 - Royal Society of Chemistry | Food & Function
Nelson Andrade, Sara Andrade, Cláudia Silva, Ilda Rodrigues, Luísa Guardão, João Tiago Guimarães, Elisa Keating, Fátima Martel,
Tópico(s): Metabolism, Diabetes, and Cancer
2019 - Springer Science+Business Media | European Journal of Nutrition
Produção Nacional
Revisado por pares
GeraldoBezerra da Silva, Nattachai Srisawat, Gabriela Studart Galdino, ÊnioSimas Macedo, José Pinto, GeysaMaria Nogueira Farias, RenanLima Alencar, Roberto da Justa Pires Neto, ElvinoJosé Guardão Barros, Elizabeth De Francesco Daher,
Leptospirosis is a bacterial disease disseminated through the centuries in the whole world which causes symptoms that go from self-limited diseases to hemorrhagic manifestations and organ failure, including acute kidney injury (AKI), composing the severe disease known as the Weil's syndrome. Mortality rates varies according to the clinical presentation and usually increases when kidney injury is present, and is even higher in the setting of pulmonary hemorrhage. There are recent advances in the search for novel ...
Tópico(s): Viral Infections and Vectors
2018 - Elsevier BV | Asian Pacific Journal of Tropical Medicine
Manuela Amorim, Cláudia Marques, Joana Odila Pereira, L. Guardão, Maria João Martins, Hugo Osório, D. Moura, Conceição Calhau, Hélder Pinheiro, Manuela Pintado,
Numerous studies have investigated dietary approaches to prevent chronic lifestyle-related diseases, including hypertension. Spent brewer’s yeast is the second largest byproduct originated by the brewing industry and it deserves considerable attention because of its high nutritional value, ca. 40% of its dry mass is rich in protein which can be hydrolyzed into biologically active peptides. To upgrade this byproduct, the aim of this study was initially in vitro assessment of biological properties, ...
Tópico(s): Biochemical effects in animals
2018 - Elsevier BV | Process Biochemistry
Viviana Pinto, Raquel Costa‐Almeida, Ilda Rodrigues, Luísa Guardão, Raquel Soares, Rui Miranda Guedes,
Abstract Anterior cruciate ligament (ACL) reconstructive surgeries are the most frequent orthopedic procedures in the knee. Currently, existing strategies fail in completely restoring tissue functionality and have a high failure rate associated, presenting a compelling argument towards the development of novel materials envisioning ACL reinforcement. Tendons and ligaments, in general, have a strong demand in terms of biomechanical features of developed constructs. We have previously developed polylactic ...
Tópico(s): Knee injuries and reconstruction techniques
2017 - Wiley | Journal of Biomedical Materials Research Part A
Produção Nacional
Revisado por pares
Geraldo Bezerra da Silva, José Pinto, Elvino José Guardão Barros, Geysa Maria Nogueira Farias, Elizabeth De Francesco Daher,
Malaria is an infectious disease of great importance for Public Health, as it is the most prevalent endemic disease in the world, affecting millions of people living in tropical areas of the globe. Kidney involvement is relatively frequent in infections by P. falciparum and P. malariae, but has also been described in the infection by P. vivax. Kidney complications in malaria mainly occur due to hemodynamic dysfunction and immune response. Liver complications leading to hepatomegaly, jaundice and ...
Tópico(s): Parasites and Host Interactions
2017 - UNIVERSIDADE DE SÃO PAULO | Revista do Instituto de Medicina Tropical de São Paulo
Rita Dias Guardão Franca, Joana Ortigueira, H.M. Pinheiro, Nídia D. Lourenço,
Treatment of the highly polluting and variable textile industry wastewater using aerobic granular sludge (AGS) sequencing batch reactors (SBRs) has been recently suggested. Aiming to develop this technology application, two feeding strategies were compared regarding the capacity of anaerobic–aerobic SBRs to deal with disturbances in the composition of the simulated textile wastewater feed. Both a statically fed, anaerobic–aerobic SBR and an anaerobic plug-flow fed, anaerobic–aerobic SBR could cope ...
Tópico(s): Chromium effects and bioremediation
2017 - Pergamon Press | Water Science & Technology
Produção Nacional
Revisado por pares
Geraldo Bezerra da Silva, Adolfo Gomes Vasconcelos, Amanda Maria Timbó Rocha, Vanessa Ribeiro de Vasconcelos, João de Barros Neto, Julye Sampaio Fujishima, Nathália Barros Ferreira, Elvino José Guardão Barros, Elizabeth De Francesco Daher,
Bee stings can cause severe reactions and have caused many victims in the last years. Allergic reactions can be triggered by a single sting and the greater the number of stings, the worse the prognosis. The poisoning effects can be systemic and can eventually cause death. The poison components are melitin, apamin, peptide 401, phospholipase A2, hyaluronidase, histamine, dopamine, and norepinephrine, with melitin being the main lethal component. Acute kidney injury (AKI) can be observed in patients ...
Tópico(s): Venomous Animal Envenomation and Studies
2017 - UNIVERSIDADE DE SÃO PAULO | Revista do Instituto de Medicina Tropical de São Paulo
Diogo Pestana, Diana Teixeira, Manuela Meireles, Cláudia Marques, Sónia Norberto, Carla Sá, Virgínia Cruz Fernandes, Luísa Correia‐Sá, Ana Faria, Luísa Guardão, João Tiago Guimarães, Wendy N. Cooper, Ionel Sandovici, Valentina F. Domingues, Cristina Delerue‐Matos, Rosário Monteiro, Miguel Constância, Conceição Calhau,
Abstract Endocrine-disrupting chemicals such as p , p ’-dichlorodiphenyldichloroethylene ( p , p ’-DDE), are bioaccumulated in the adipose tissue (AT) and have been implicated in the obesity and diabetes epidemic. Thus, it is hypothesized that p , p ’-DDE exposure could aggravate the harm of an obesogenic context. We explored the effects of 12 weeks exposure in male Wistar rats’ metabolism and AT biology, assessing a range of metabolic, biochemical and histological parameters. p , p ’-DDE -treatment exacerbated several ...
Tópico(s): Diet and metabolism studies
2017 - Nature Portfolio | Scientific Reports
Produção Nacional
Revisado por pares
Ida S. Sano‐Martins, Alaour Candida Duarte, Belsy Guerrero, Roberto Henrique Pinto Moraes, Elvino José Guardão Barros, Carmen Luisa Arocha-Piñango,
Patients envenomed by Lonomia sp caterpillars initially experience a mild burning pain, headache, nausea, vomiting, and skin and mucosal hemorrhages. Some patients can rapidly progress to a severe coagulopathy that presents as visceral or intracerebral hemorrhaging. We studied the hemostatic alterations that occurred in 14 patients who were envenomed by Lonomia obliqua in Southern Brazil and presented at the Hospital São Vicente de Paulo (Passo Fundo, RS), Brazil during the summers of 1993 and 1994 ...
Tópico(s): Allergic Rhinitis and Sensitization
2017 - UNIVERSIDADE DE SÃO PAULO | Revista do Instituto de Medicina Tropical de São Paulo
Raquel Costa, Ilda Rodrigues, Luísa Guardão, Sílvia Rocha‐Rodrigues, Carolina Silva, José Magalhães, Manuel Ferreira-de-Almeida, Rita Negrão, Raquel Soares,
Type 2 diabetes mellitus (T2DM) is a chronic disease characterized by metabolic disturbances in specific tissues. The present work aimed to analyze the effects of xanthohumol (XN) and 8-prenylnaringenin (8PN), two beer-derived polyphenols, in liver and skeletal muscle lipid and glycolytic metabolism in T2DM mice model. Thirty C57Bl/6 mice were randomly divided into five groups: standard diet (control), high-fat diet (DM), high-fat diet plus ethanol (DM-Ethanol), high-fat diet plus 10 mg/L XN (DM-XN) and ...
Tópico(s): Toxin Mechanisms and Immunotoxins
2017 - Elsevier BV | The Journal of Nutritional Biochemistry
Rita Dias Guardão Franca, H.M. Pinheiro, Mark C.M. van Loosdrecht, Nídia D. Lourenço,
Aerobic granular sludge technology has been extensively studied over the past 20 years and is regarded as the upcoming new standard for biological treatment of domestic and industrial wastewaters. Aerobic granules (AG) are dense, compact, self-immobilized microbial aggregates that allow better sludge-water separation and thereby higher biomass concentrations in the bioreactor than conventional activated sludge aggregates. This brings potential practical advantages in terms of investment cost, energy ...
Tópico(s): Constructed Wetlands for Wastewater Treatment
2017 - Elsevier BV | Biotechnology Advances
Clarisse Ribeiro, Daniela M. Correia, Ilda Rodrigues, L. Guardão, Sérgio Augusto Catanzaro Guimarães, Raquel Soares, S. Lanceros‐Méndez,
The potential of piezoelectric biomaterials for bone tissue engineering is demonstrated. This work proves that the use of piezoelectric poly(vinylidene fluoride) (PVDF), able to provide electrical stimuli upon mechanical solicitation to the growing cells, enhances bone regeneration in vivo. Poled and non-poled β-PVDF films and randomly oriented electrospun fiber mats have been used to test their osteogenic properties in Wistar rats by analyzing new bone formation in vivo. After 4 weeks, defect implanted ...
Tópico(s): Electrospun Nanofibers in Biomedical Applications
2017 - Elsevier BV | Materials Letters
Raquel Costa, Ilda Rodrigues, Luísa Guardão, Joana Quelhas Lima, Emı́lia Sousa, Raquel Soares, Rita Negrão,
Scope Imbalance in kidney and heart neovascularization is common in type2 diabetes (T2DM) patients. Nevertheless, the mechanisms governing this angiogenic paradox have not been elucidated. Xanthohumol (XN) and 8‐prenylnaringenin (8PN) beer polyphenols modulate angiogenesis, being thus targets for T2DM‐related complications. Our work examined whether polyphenols consumption affects angiogenic paradox and metabolism in a T2DM mouse model. Methods and results An increase in kidney and a reduction in left ...
Tópico(s): Kruppel-like factors research
2016 - Wiley | Molecular Nutrition & Food Research
Rita Dias Guardão Franca, A. Vieira, Ana Maria Tavares da Mata, Gilda Carvalho, H.M. Pinheiro, Nídia D. Lourenço,
This study analyzed the effect of an azo dye (Acid Red 14) on the performance of an aerobic granular sludge (AGS) sequencing batch reactor (SBR) system operated with 6-h anaerobic-aerobic cycles for the treatment of a synthetic textile wastewater. In this sense, two SBRs inoculated with AGS from a domestic wastewater treatment plant were run in parallel, being one supplied with the dye and the other used as a dye-free control. The AGS successfully adapted to the new hydrodynamic conditions forming ...
Tópico(s): Constructed Wetlands for Wastewater Treatment
2015 - Elsevier BV | Water Research
Produção Nacional
Revisado por pares
Geraldo Bezerra da Silva, Elizabeth De Francesco Daher, Roberto da Justa Pires Neto, Eanes Delgado Barros Pereira, Gdayllon Cavalcante Meneses, Sônia Maria Holanda Almeida Araújo, Elvino José Guardão Barros,
Leprosy is a chronic disease caused by Mycobacterium leprae, highly incapacitating, and with systemic involvement in some cases. Renal involvement has been reported in all forms of the disease, and it is more frequent in multibacillary forms. The clinical presentation is variable and is determined by the host immunologic system reaction to the bacilli. During the course of the disease there are the so called reactional states, in which the immune system reacts against the bacilli, exacerbating the ...
Tópico(s): Mycobacterium research and diagnosis
2015 - UNIVERSIDADE DE SÃO PAULO | Revista do Instituto de Medicina Tropical de São Paulo
Nídia D. Lourenço, Rita Dias Guardão Franca, M.A. Carmo Moreira, Fátima N. Gil, Cristina A. Viegas, H.M. Pinheiro,
Textile wastewaters are rated among the most polluting in all industrial sectors, including high organic loads and dyes that are generally resistant to aerobic biotreatment. Alternative, staged anaerobic/aerobic regimes have been tested, with clear success in the biodecolorization of the widely used azo dyes. The novel aerobic granular sludge (AGS) technology has excellent potential in this context, due to the anoxic/anaerobic zones within granules and their increased tolerance to toxicity. In this ...
Tópico(s): Chromium effects and bioremediation
2015 - Elsevier BV | Biochemical Engineering Journal
Elisa Keating, Ana Correia‐Branco, João R. Araújo, Manuela Meireles, Rita Fernandes, Luísa Guardão, João Tiago Guimarães, Fátima Martel, Conceição Calhau,
The aim of this study was to understand whether high folic acid (HFA) exposure during the perigestational period induces metabolic dysfunction in the offspring, later in life. To do this, female Sprague-Dawley rats (G0) were administered a dose of folic acid (FA) recommended for pregnancy (control, C, 2 mg FA/kg of diet, n=5) or a high dose of FA (HFA, 40 mg FA/kg of diet, n=5). Supplementation began at mating and lasted throughout pregnancy and lactation. Body weight and food and fluid intake were monitored ...
Tópico(s): Folate and B Vitamins Research
2015 - Bioscientifica | Journal of Endocrinology
Produção Nacional
Revisado por pares
Geraldo Bezerra da Silva, Elvino José Guardão Barros, Elizabeth De Francesco Daher,
Leishmaniasis is an infectious disease caused by protozoa of the genus Leishmania transmitted by insects of the genus Lutzomyia sp. or Phlebotomus sp. The main syndromes are cutaneous leishmaniasis, mucocutaneous leishmaniasis, visceral leishmaniasis (kala-azar) and post-kala-azar dermal leishmaniasis. This article reviews kidney involvement in cutaneous and visceral leishmaniasis, highlighting the aspects of their pathophysiology, clinical manifestations, histopathological findings, outcome and ...
Tópico(s): Blood Coagulation and Thrombosis Mechanisms
2014 - Elsevier BV | The Brazilian Journal of Infectious Diseases
Produção Nacional
Revisado por pares
Elizabeth De Francesco Daher, Geraldo Bezerra da Silva, Elvino José Guardão Barros,
Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis . The disease remains as an important public health problem in developing countries. Extrapulmonary TB became more common with the advent of infection with human immunodeficiency virus and by the increase in the number of organ transplantation, which also leads to immunosuppression of thousand of persons. Urogenital TB represents 27% of extrapulmonary cases. Renal involvement in TB can be part of a disseminated infection or a localized ...
Tópico(s): Mycobacterium research and diagnosis
2013 - American Society of Tropical Medicine and Hygiene | American Journal of Tropical Medicine and Hygiene
Raquel Costa, Rita Negrão, Inês M. Valente, Ângela Castela, Delfim Duarte, Luísa Guardão, Paulo J. Magalhães, José A. Rodrigues, João Tiago Guimarães, Pedro Gomes, Raquel Soares,
Type 1 diabetes mellitus is responsible for metabolic dysfunction, accompanied by chronic inflammation, oxidative stress, and endothelium dysfunction, and is often associated with impaired wound healing. Phenol-rich food improves vascular function, contributing to diabetes prevention. This study has evaluated the effect of phenol-rich beverage consumption in diabetic rats on wound healing, through angiogenesis, inflammation, and oxidative stress modulation. A wound-healing assay was performed in ...
Tópico(s): Horticultural and Viticultural Research
2013 - American Chemical Society | Journal of Natural Products
Produção Nacional
Revisado por pares
Geraldo Bezerra da Silva, Daniella Bezerra Duarte, Elvino José Guardão Barros, Elizabeth De Francesco Daher,
Schistosomiasis is a parasitic disease caused by organisms from the genus Schistosoma. The disease is endemic in tropical areas, where there are currently millions of people living in areas with transmission risk. Schistosomiasis-associated kidney disease is not frequently described in literature. The disease has a chronic evolution, with variable severity. Glomerulonephritis is described in 10–12% in autopsy studies. Proteinuria is reported in 20% of patients with S. mansoni infection. Schistosomal ...
Tópico(s): Research on Leishmaniasis Studies
2013 - Asian Pacific Journal of Tropical Disease Editorial Office | Asian Pacific Journal of Tropical Disease
Produção Nacional
Revisado por pares
Bárbara Margareth Menardi Biavo, Carmen Tzanno‐Martins, Lucas Maciel Cunha, Melissa Luciana de Araújo, Márcia Machado Cunha Ribeiro, Anita Sachs, Clarissa Baia Bargas Uezima, Sérgio Antônio Draibe, Cibele Isaac Saad Rodrigues, Elvino José Guardão Barros,
INTRODUÇÃO: O Comitê de Nutrição da Sociedade Brasileira de Nefrologia (SBN) realizou, em 2010, o primeiro Censo Brasileiro de Nutrição em pacientes em Hemodiálise. Dados multicêntricos contribuem para o desenvolvimento de condutas clínicas e intervenção nutricional. OBJETIVO: Descrever aspectos nutricionais e epidemiológicos de pacientes em hemodiálise. MÉTODO: Estudo transversal em 36 clínicas de diálise, 2.622 participantes selecionados aleatoriamente. Foram coletados: registros sociodemográficos, ...
Tópico(s): Public Health in Brazil
2012 - Sociedade Brasileira de Nefrologia | Brazilian Journal of Nephrology
Renata Aparecida Nedel Pértile, Susana Moreira, Rui M. Gil da Costa, Alexandra Correia, Luísa Guardão, Fátima Gärtner, Manuel Vilanova, Miguel Gama,
Abstract The bacterial cellulose (BC) secreted by Gluconacetobacter xylinus is a network of pure cellulose nanofibres which has high crystallinity, wettability and mechanical strength. These characteristics make BC an excellent material for tissue-engineering constructs, noteworthy for artificial vascular grafts. In this work, the in vivo biocompatibility of BC membranes produced by two G. xylinus strains was analyzed through histological analysis of long-term subcutaneous implants in the mice. The ...
Tópico(s): biodegradable polymer synthesis and properties
2012 - Taylor & Francis | Journal of Biomaterials Science Polymer Edition
Renata Ramalho, Joana Almeida, Marília Beltrão, Ana Pirraco, Raquel Costa, Oksana Sokhatska, Luísa Guardão, Carmo Palmares, João Tiago Guimarães, Luís Delgado, André Moreira, Raquel Soares,
A number of studies have shown an association between obesity and asthma. Controversy remains on the mechanisms supporting this association. In this study we aimed to assess neurogenic inflammation in a model of diet-induced obesity and allergen-challenged mice.High fat diet-induced (HFD) obese Balb/c mice were sensitized and challenged with ovalbumin (OVA). Glucose, insulin, OVA-specific IgE and substance P (SP), and the main tachykinin involved in neurogenic inflammation, were quantified in sera. ...
Tópico(s): Respiratory and Cough-Related Research
2012 - Informa | Experimental Lung Research